CN103333836A - Bacterium for degrading herbicides chlorimuron-ethyl and acetochlor and application of bacterium - Google Patents
Bacterium for degrading herbicides chlorimuron-ethyl and acetochlor and application of bacterium Download PDFInfo
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Abstract
The invention discloses a bacterium for degrading herbicides chlorimuron-ethyl and acetochlor and application of the bacterium. The bacterium is cellulosimicrobium sp. LY114, which is preserved in China General Microbiological Culture Collection Center by a preservation number of CGMCC No.7776. The cellulosimicrobium sp. LY114 CGMCC No.7776 reaches a degrading rate of 89.11% on chlorimuron-ethyl (45mg/L) and acetochlor (45mg/L) in an organic salt culture medium within 7d, showing that the strain can effectively degrade the chlorimuron-ethyl and the acetochlor; and the bacterium has a wide application prospect in an aspect of repairing chlorimuron-ethyl and acetochlor pollution of soil.
Description
Technical field
The present invention relates to bacterium of a strain degrading herbicide chlorimuronethyl and acetochlor and uses thereof.
Background technology
Chlorimuronethyl, english common name chlorimuron-ethyl, chemical name 2-(4-chloro-6-methoxy pyrimidine-2-base carbamyl amino-sulfonyl) methyl benzoate, structural formula be as shown in Equation 1:
Chlorimuronethyl is the sulfonylurea herbicide of ultra-high efficiency, action target is the ALS (acetolactate synthestase) in the plant materials, suppress the biosynthesizing of branched-chain amino acid Xie Ansuan, Isoleucine, cause substrate α-butanone accumulation, block cell interkinesis DNA is synthetic, mitotic division is stopped, and cell can not normal growth.Chlorimuronethyl can be absorbed by the root of plant, stem, leaf, conducts up and down in plant materials, is before the selectivity bud, post-emergence herbicide, is mainly used in farmlands such as soybean, prevents and kill off broadleaf weeds and some Cyperaceae and gramineous weeds.
Acetochlor, english common name acetochlor, chemical name 2-ethyl-6 methyl--N-ethoxyl methyl-alpha-chloro acetanilide N, structural formula be as shown in Equation 2:
Acetochlor is interior absorption acetamide-group herbicides, is one of several herbicides of consumption maximum in the agriculture production.Can be absorbed by weeds young shoot and young root, suppress the weeds protein synthesis, and make weeds death.Be used for various vegetables field such as corn, cotton, soybean, peanut, rape, potato, sugarcane, sesame, Sunflower Receptacle and pulse family, Cruciferae, Solanaceae, composite family, Carrot family and orchard and prevent and kill off annual gramineous weed and part broadleaf weeds, the soybean South Dodder Seed Chinese Dodder Seed there is good preventive effect, invalid to perennial weeds, lasting period can reach 2 months in soil, and a dispenser can be controlled the whole crop growth phase does not have weeds harm.
A large amount of uses of chemical herbicide improve agricultural production efficiency greatly, but very big production and environmental problem have also been brought simultaneously, poisoning to succession crop is on the rise as long residual effect weedicide, influences the structural adjustment of agricultural planting industry, causes heavy losses to agriculture production.Chlorimuronethyl has advantages such as consumption is few, activity is high, herbicidal effect is good, firmly getting vast farmers likes, but chlorimuronethyl is release weedicide, the longevity of residure is longer in soil, can reach 2-3, all can produce in various degree poisoning to succession crop such as beet, potato, melon, jowar, rape, corn etc., make that plant-growth is slow, vegetative point is downright bad, branch and basal part of stem is aging, internode shortens, plant is downgraded, the ripening stage postpones, even cause crop death.Plant Sunflower Receptacle again the soybean Tanaka who used chlorimuronethyl, will cause the total crop failure of being injured of a back stubble Sunflower Receptacle big area.
The degraded of natural herbicide residue is mainly finished by microorganism in the soil, but natural degradation is very slow.Therefore, screen the high-effective microorganism bacterial strain targetedly, the development microorganism renovation agent, the residual poisoning of degraded elimination farmland herbicide of accelerating the soil lasting rudimental herbicide by artificial inoculation is a very necessary job and practicable approach.
Summary of the invention
The purpose of this invention is to provide a strain can efficient degradation weedicide chlorimuronethyl and the bacterium of acetochlor.
Bacterium provided by the present invention is fiber germ (Cellulosimicrobium sp.) LY1114, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on June 20th, 2013 and (is called for short CGMCC, the address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City), deposit number is CGMCC No.7776.
Another object of the present invention provides a kind of microbial inoculum, and the activeconstituents of this microbial inoculum is described fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776.
Described microbial inoculum is following 1) or 2) described microbial inoculum:
1) is used for the microbial inoculum of degrading herbicide chlorimuronethyl and/or acetochlor;
2) be used for the microbial inoculum that the residual weedicide chlorimuronethyl of rehabilitating soil and/or acetochlor pollute.
Described microbial inoculum also can comprise carrier.Described carrier can be solid carrier or liquid vehicle.Described solid carrier is mineral material, vegetable material or macromolecular compound; Described mineral material is at least a in clay, talcum, kaolin, montmorillonite, white carbon, zeolite, silica and the diatomite; Described vegetable material is at least a in Semen Maydis powder, bean powder and the starch; Described macromolecular compound is polyvinyl alcohol and/or polyglycol.Described liquid vehicle is organic solvent, vegetables oil, mineral oil or water; Described organic solvent is decane and/or dodecane.In the described microbial inoculum, described activeconstituents can be to be existed by the form of the mixture of the filtrate of the fermented liquid of the viable cell cultivated, viable cell, cell culture or cell and filtrate.The formulation of described composition can be multiple formulation, as liquor, emulsion, suspension agent, pulvis, granule, wettable powder or water dispersible granules.
As required, also can add tensio-active agent (as polysorbas20, tween 80 etc.), tackiness agent, stablizer (as antioxidant), pH regulator agent etc. in the described microbial inoculum.
Described fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 or be that the application of microbial inoculum in degrading herbicide chlorimuronethyl and/or acetochlor of activeconstituents also belongs to protection scope of the present invention with fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776.
Described fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 or be that the application of microbial inoculum in the residual weedicide chlorimuronethyl of rehabilitating soil and/or acetochlor pollute of activeconstituents also belongs to protection scope of the present invention with fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776.
A further object of the present invention provides the method for a kind of cultivation fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776, and this method comprises the step that fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 is cultivated at the substratum that is used for cultivation fiber germ.
It is the preparation method of the microbial inoculum of activeconstituents with fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 that another purpose of the present invention provides a kind of, comprise the steps: described fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 is obtained described microbial inoculum as activeconstituents.
The present invention gathers soil sample from the farmland that is subjected to the pollution of residual weedicide chlorimuronethyl and acetochlor for a long time, and therefrom separates, screens the degrading herbicide chlorimuronethyl and acetochlor bacterial strain fiber germ (Cellulosimicrobium sp.) the LY1114CGMCC No.7776 that obtain.Experiment showed, that the chlorimuronethyl of this bacterial strain 7d in minimal medium (45mg/L) degradation rate reaches 89.11%; Acetochlor (45mg/L) degradation rate is reached 32.08%.This shows that this bacterial strain energy efficient degradation weedicide chlorimuronethyl and acetochlor are having broad application prospects aspect the residual weedicide chlorimuronethyl of rehabilitating soil and the acetochlor pollution.
The preservation explanation
Strain name: fiber germ
Latin name: (Cellulosimicrobium sp.)
Strain number: LY1114
Preservation mechanism: China Committee for Culture Collection of Microorganisms common micro-organisms center
Preservation mechanism is called for short: CGMCC
Address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City
Preservation date: on June 20th, 2013
The preservation center numbering of registering on the books: CGMCC No.7776
Embodiment
Employed experimental technique is ordinary method if no special instructions among the following embodiment.
Used material, reagent etc. if no special instructions, all can obtain from commercial channels among the following embodiment.
Enrichment culture liquid: K
2HPO
47g/L, KH
2PO
42g/L, MgSO
47H
2O0.1g/L, MnSO
40.05g/L, FeSO
40.05g/L, CaC1
20.003g/L glucose 5g/L is settled to 1L with distilled water, pH7.0; (chlorimuronethyl and acetochlor consumption are according to hereinafter embodiment 1 interpolation).
Isolation medium: adding agar to its mass concentration in the enrichment culture liquid is 2%.
Inorganic salt liquid substratum: NH
4Cl1.0g/L, KH
2PO
40.5g/L, K
2HPO
41.5g/L, MgSO
40.2g/L NaCl0.5g/L is settled to 1L with distilled water, pH7.0.
The inorganic salt solid medium: adding agar to its mass concentration in the inorganic salt liquid substratum is 2%.
Beef-protein medium: extractum carnis 5g/L, peptone 10g/L, NaCl5g/L, agar 20g/L is settled to 1L with distilled water, pH7.2.
Separation and the evaluation of embodiment 1, fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776
One, the separation of herbicide degradation bacterium LY1114
Contain at 100mL and to add 10g pedotheque (pick up from Chinese Shandong Province and be subjected to the farmland that the weedicide acetochlor pollutes), 28 ℃, 180r/min shaking culture 7d enrichment chlorimuronethyl degradation bacteria in the enrichment culture liquid of chlorimuronethyl 20mg/L.After first round enrichment finishes, draw the 10mL pregnant solution and be transferred in the 100mL enrichment culture liquid that contains chlorimuronethyl 40mg/L, continuation cultivation 7d carries out second and takes turns enrichment.So continuously enrichment, cultivate 5 times, chlorimuronethyl concentration is followed successively by 20,40,60,80 and 100mg/L.
Dull and stereotyped transparent circle method is adopted in the screening of degradation bacteria.Chlorimuronethyl is added the inorganic salt solid medium make flat board, final concentration is 1000mg/L.Flat board is divided into four districts, and the different samples of every district mark (pregnant solution) numbering is got pregnant solution 15 μ L dibblings to numbering area, 28 ℃ of cultivation, observation.The sample (pregnant solution) that will have transparent circle to occur becomes 10 with the sterilized water doubling dilution
-1, 10
-2, 10
-3, 10
-4, 10
-5Gradient gets 10
-3, 10
-4, 10
-5Each 100 μ L of gradient are to the inorganic salt solid medium of above-mentioned chloride sulfometuron-methyl 1000mg/L, and 3 repetitions of each gradient, coating are evenly.28 ℃ of cultivations, observe, treat dull and stereotyped go up single bacterium colony appears and after, picking has single bacterium colony of transparent circle to be forwarded on the beef-protein medium, 25 ℃ of cultivations, purifying, standby.
Through a large amount of enrichment culture, being separated to can be in strain surplus the bacterial strain 60 of the inorganic salt plate culture medium formation transparent circle that contains chlorimuronethyl 1000mg/L.Further after the screening, obtain a bacterial strain that is numbered LY1114, called after herbicide degradation bacterium LY1114, this bacterial strain can degrade 89.11% with the chlorimuronethyl of starting point concentration 45mg/L in 7d under the pure culture condition; And the acetochlor of can also degrading, 7d is degraded to 32.08% with the acetochlor of starting point concentration 45mg/L.
Two, the evaluation of herbicide degradation bacterium LY1114
From the herbicide degradation bacterium LY1114 that the following aspects authentication step one is separated and purifying obtains:
1, morphology is identified
To be in logarithmic phase, and the bacterium colony size is stable, the herbicide degradation bacterium LY1114 that above-mentioned steps one is separated and purifying obtains carries out single bacterium colony state observation, mainly comprises size, color, transparency, wettability, bacterium colony condition of surface (whether smooth, projection, fold, depression etc.), the colony edge state (whether neat, irregular, radial etc.) of bacterium colony.
For the described herbicide degradation bacterium LY1114 that is in logarithmic phase, behind smear staining, adopt the form of observation by light microscope thalline.
The result shows, the herbicide degradation bacterium LY1114 bacterium colony circular protrusions that above-mentioned steps one is separated and purifying obtains, faint yellow, smooth surface is moistening, neat in edge; The thalline rod-short, Gram-positive.
2, analysis of physio biochemical characteristics
With reference to " common bacteria system identification handbook " (eastern elegant pearl, Cai Miaoying. common bacteria system identification handbook. Beijing: Science Press, 2011.) and " microbiology experiment " (Shen Ping, Fan Xiurong, Li Guangwu. microbiology experiment (third edition). Beijing: Higher Education Publishing House, 1999.) measure the physiological and biochemical property of above-mentioned herbicide degradation bacterium LY1114.
The physiological and biochemical property measurement result of described herbicide degradation bacterium LY1114 is as shown in table 1:
The physiological and biochemical property of table 1 herbicide degradation bacterium LY1114
Annotate: "+" expression is positive, and "-" expression is negative.
3,16s rDNA sequence homology analysis
Ordinary method is cultivated the herbicide degradation bacterium LY1114 that above-mentioned steps one separation and purification obtains, extract total DNA of bacterial strain as the gene amplification template, with bacterium 16s rDNA universal primer, 27f:5 '-AGAGTTTGATCCTGGCTCAG-3 ', 1492r:5 '-TACCTTGTTACGACTT-3 ' carry out the PCR reaction.Reaction system adopts Shanghai biotechnology company limited pcr amplification test kit.Response procedures is: 95 ℃ of sex change 30s, 55 ℃ of annealing 1min, 72 ℃ of extensions 2min, totally 30 circulations.Dna sequencing is finished by Beijing three rich polygala root biotech companies, sequence assembly and similarity analysis use DNAStar software to finish, and gene is compared by American National biotechnology information center ncbi database (http://www.ncbi.nlm.nih.gov) and EzTaxon is online finishes.
The pcr gene amplification obtains the about 1.4kb of 16S rDNA gene fragment of herbicide degradation bacterium LY1114, measure after the sequence with NCBI and Ez Taxon database in disclosed 16S rDNA sequence carry out online homology comparison, the result shows LY1114 and funk fiber germ Cellulosimicrobium funkei ATCC BAA-886
TThe homology of (GenBank accession number AY501364) is the highest, reaches 99.709%.
The 16s rDNA sequence of herbicide degradation bacterium LY1114 sees sequence 1 in the sequence table for details.
In view of above-mentioned form, analysis of physio biochemical characteristics and 16s rDNA sequence homology analysis result, the herbicide degradation bacterium LY1114 that the step 1 separation and purification is obtained is accredited as bacterium territory actinomycete group micrococcaceae (Micrococcineneae) fiber germ genus (Cellulosimicrobium sp.).This herbicide degradation bacterium LY1114 is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on June 20th, 2013 and (is called for short CGMCC, the address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City), deposit number is CGMCC No.7776.
Embodiment 2, fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 degrading herbicide chlorimuronethyl and the quantitative assay of acetochlor ability
One, chlorimuronethyl bioassay standard curve plotting
With methyl alcohol chlorimuronethyl standard substance (available from Fluka company) are mixed with series concentration 10,20,40,60,80,100mg/L standardized solution, adopt high performance liquid chromatography (HPLC) to measure the peak area of different concns chlorimuronethyl standard substance, 3 repetitions.Concentration with chlorimuronethyl is X-coordinate, and peak area is ordinate zou, draws the chlorimuronethyl typical curve.
Testing conditions is as follows:
Detection system: Agilent1100Series.Chromatographic column: C18Diamosil
TMReversed-phase column, 250mm * 4.6mm, particle diameter 5 μ m.Chromatographic condition: moving phase: acetonitrile: methyl alcohol: water: glacial acetic acid=45:15:40:0.1(v/v); Detect wavelength, 236nm; Flow velocity, 1.0mL/min; Sampling volume, 10 μ L; 30 ℃ of column temperatures.
Measurement result is as shown in table 2:
Table 2 different concns chlorimuronethyl HPLC measures peak area
According to table 2 data, obtain chlorimuronethyl bioassay standard curve: y=33.717x-20.032 (R
2=0.9950).Wherein, y is peak area, and x is chlorimuronethyl concentration.
Two, acetochlor bioassay standard curve plotting
With methyl alcohol acetochlor standard substance (available from Fluka company) are mixed with series concentration 10,20,40,60,80,100mg/L standardized solution, adopt high performance liquid chromatography (HPLC) to measure the peak area of different concns acetochlor standard substance, 3 repetitions.Concentration with acetochlor is X-coordinate, and peak area is ordinate zou, draws the acetochlor typical curve.
Testing conditions is as follows:
Detection system: Agilent1100Series.Chromatographic column: C18Diamosil
TMReversed-phase column, 250mm * 4.6mm, particle diameter 5 μ m.Chromatographic condition: moving phase: methyl alcohol: water=80:20(v/v), water transfers to pH=3 with Glacial acetic acid; Detect wavelength, 215nm; Flow velocity, 1.0mL/min; Sampling volume, 20 μ L; 30 ℃ of column temperatures.
Measurement result is as shown in table 3:
Table 3 different concns acetochlor HPLC measures peak area
According to table 3 data, obtain acetochlor bioassay standard curve: y=51.44x+1615 (R
2=0.999).Wherein, y is peak area, and x is acetochlor concentration.
Three, fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 degraded chlorimuronethyl ability quantitative assay
The accurate 5mL of adding contains the minimal medium (adding the substratum that chlorimuronethyl makes the concentration of chlorimuronethyl obtain for about 50mg/L in the inorganic salt liquid substratum) of the about 50mg/L of chlorimuronethyl in test tube, adds fiber germ (Cellulosimicrobium sp.) the LY1114CGMCC No.7776 bacterium liquid (1 * 10 of OD600nm=1.0 again
9Cfu/ml) 0.2mL, 28 ℃, 180r/min were cultivated 7 days, got the 4mL degradation solution to the 50mL centrifuge tube, added the 4mL methylene dichloride, and vibration 2min leaves standstill 10min, adds a little anhydrous sodium sulphate.Accurately drawing 500 μ L organic phases is transferred in the 1.5mL EP centrifuge tube, dry up at Nitrogen evaporator, add 500 μ L methyl alcohol (chromatographic grade), dissolve 10min at ultrasonic washing instrument, be collected in the sample bottle with the filtration of liquid spectrum strainer, measure chlorimuronethyl according to above-mentioned HPLC method.Simultaneously, as blank, measure the concentration of chlorimuronethyl with the above-mentioned minimal medium that contains the about 50mg/L of chlorimuronethyl of not inoculating fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 according to the method described above.
Chlorimuronethyl degradation rate: X=(1-A/B) * 100%, X is degradation rate (%) in the formula, and A connects chlorimuronethyl concentration residual in the bacterium treatment solution, and B is not for connecing chlorimuronethyl concentration residual in the bacterium blank treatment solution.3 repetitions are established in experiment, repeat each at every turn and handle 1 test tube of inoculation.
The result shows that the chlorimuronethyl starting point concentration is 45.09mg/L, after 7 days the degradation rate of the chlorimuronethyl of described fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 to reach 89.11%(as shown in table 4).This result shows, fiber germ provided by the present invention (Cellulosimicrobium sp.) but LY1114CGMCC No.7776 efficient degradation chlorimuronethyl.
Table 4 fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 degraded chlorimuronethyl effect
Four, fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 degraded acetochlor ability quantitative assay
Contain fiber germ (Cellulosimicrobium sp.) the LY1114CGMCC No.7776 bacterium liquid (1 * 10 that inserts OD600nm=1.0 in the triangular flask (volume is 250ml) of the minimal medium of the about 50mg/L of acetochlor at 50mL
9Cfu/ml) 2mL, 30 ℃, the 200r/min shaking table was cultivated 7 days, measured the acetochlor residual quantity.Method is: get the 3mL degradation solution to the 50mL centrifuge tube, the centrifugal 5min of 8000r/min collects supernatant liquor, add the 3mL methylene dichloride, thermal agitation 5min, leave standstill 10min, treat water and organic phase layering, adding a small amount of anhydrous sodium sulphate dewaters to organic phase, accurately draw 800 μ L organic phases in the centrifuge tube of 1.5mL, dry up with Nitrogen evaporator, add 400 μ L methyl alcohol, ultrasonic 10min in ultrasonic cleaner, organic phase aculeus type filter with 0.22 μ m is filtered to the liquid chromatography sample bottle, measures acetochlor according to above-mentioned HPLC method.Simultaneously, as blank, measure the concentration of acetochlor with the above-mentioned minimal medium that contains the about 50mg/L of acetochlor that do not have incoming fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 according to the method described above.Acetochlor degradation rate: X=(1-A/B) * 100%, X is degradation rate (%) in the formula, and A connects acetochlor concentration residual in the bacterium treatment solution, and B is not for connecing acetochlor concentration residual in the bacterium blank treatment solution.3 repetitions are established in experiment, repeat each at every turn and handle 1 triangular flask of inoculation.
The result shows that the acetochlor starting point concentration is 44.78mg/L, after 7 days the degradation rate of the acetochlor of described fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 to reach 32.08%(as shown in table 5).This result shows, fiber germ provided by the present invention (Cellulosimicrobium sp.) but LY1114CGMCC No.7776 efficient degradation acetochlor.
Table 5 fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 degraded acetochlor effect
Sequence table
Claims (7)
1. fiber germ (Cellulosimicrobium sp.) LY1114, its deposit number at China Committee for Culture Collection of Microorganisms common micro-organisms center is CGMCC No.7776.
2. microbial inoculum, it is characterized in that: the activeconstituents of described microbial inoculum is the described fiber germ of claim 1 (Cellulosimicrobium sp.) LY1114CGMCC No.7776.
3. microbial inoculum according to claim 2, it is characterized in that: described microbial inoculum is following 1) or 2) described microbial inoculum:
1) is used for the microbial inoculum of degraded chlorimuronethyl and/or acetochlor;
2) be used for the microbial inoculum that rehabilitating soil chlorimuronethyl and/or acetochlor pollute.
4. the described fiber germ of claim 1 (Cellulosimicrobium sp.) LY1114CGMCC No.7776 or claim 2 or the 3 described microbial inoculums application in degraded chlorimuronethyl and/or acetochlor.
5. the described fiber germ of claim 1 (Cellulosimicrobium sp.) LY1114CGMCC No.7776 or claim 2 or the 3 described microbial inoculums application in rehabilitating soil chlorimuronethyl and/or acetochlor pollution.
6. cultivate the method for the described fiber germ of claim 1 (Cellulosimicrobium sp.) LY1114CGMCCNo.7776, comprise the step that described fiber germ (Cellulosimicrobium sp.) LY1114CGMCCNo.7776 is cultivated at the substratum that is used for cultivation fiber germ.
7. the preparation method of claim 2 or 3 described microbial inoculums comprises the steps: the described fiber germ of claim 1 (Cellulosimicrobium sp.) LY1114CGMCC No.7776 is obtained described microbial inoculum as activeconstituents.
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| CN104498389A (en) * | 2014-11-26 | 2015-04-08 | 中国农业科学院农业资源与农业区划研究所 | Bacterium for degrading herbicide namely Pursuit (imazethapyr-ammonium) and acetochlor and application of bacterium |
| CN104498389B (en) * | 2014-11-26 | 2017-01-18 | 中国农业科学院农业资源与农业区划研究所 | Bacterium for degrading herbicide namely Pursuit (imazethapyr-ammonium) and acetochlor and application of bacterium |
| CN106520613A (en) * | 2014-11-26 | 2017-03-22 | 中国农业科学院农业资源与农业区划研究所 | Bacterium for degrading herbicide acetochlor and acifluorfen sodium |
| CN106520613B (en) * | 2014-11-26 | 2019-04-16 | 中国农业科学院农业资源与农业区划研究所 | The bacterium that one plant of degrading herbicide Acetochlor and weeds are burnt |
| CN104480043A (en) * | 2014-12-12 | 2015-04-01 | 中国农业科学院农业资源与农业区划研究所 | Rhodococcus sp. capable of degrading four herbicides and application thereof |
| CN104480043B (en) * | 2014-12-12 | 2017-04-12 | 中国农业科学院农业资源与农业区划研究所 | Rhodococcus sp. capable of degrading four herbicides and application thereof |
| CN112662598A (en) * | 2021-01-27 | 2021-04-16 | 新疆石大科技股份有限公司 | Fiber fingi micro-bacterium, microbial inoculum comprising fiber fingi micro-bacterium, preparation method and application |
| CN112662598B (en) * | 2021-01-27 | 2023-11-21 | 新疆石大科技股份有限公司 | Fender fiber microzyme, microbial inoculum comprising Fender fiber microzyme, preparation method and application |
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