CN103333836B - Bacterium for degrading herbicides chlorimuron-ethyl and acetochlor and application of bacterium - Google Patents
Bacterium for degrading herbicides chlorimuron-ethyl and acetochlor and application of bacterium Download PDFInfo
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Abstract
The invention discloses a bacterium for degrading herbicides chlorimuron-ethyl and acetochlor and application of the bacterium. The bacterium is cellulosimicrobium sp. LY114, which is preserved in China General Microbiological Culture Collection Center by a preservation number of CGMCC No.7776. The cellulosimicrobium sp. LY114 CGMCC No.7776 reaches a degrading rate of 89.11% on chlorimuron-ethyl (45mg/L) and acetochlor (45mg/L) in an organic salt culture medium within 7d, showing that the strain can effectively degrade the chlorimuron-ethyl and the acetochlor; and the bacterium has a wide application prospect in an aspect of repairing chlorimuron-ethyl and acetochlor pollution of soil.
Description
Technical field
The present invention relates to bacterium of a strain degrading herbicide chlorimuronethyl and acetochlor and uses thereof.
Background technology
Chlorimuronethyl, english common name chlorimuron-ethyl, chemical name 2-(4-chloro-6-methoxylpyrimidin-2-base carbamyl amino-sulfonyl) methyl benzoate, structural formula as shown in Equation 1:
Chlorimuronethyl is the sulfonylurea herbicide of ultra-high efficiency, action target is the ALS (acetolactate synthestase) in plant materials, suppress the biosynthesizing of branched-chain amino acid α-amino-isovaleric acid, Isoleucine, cause the accumulation of substrate α-butanone, block cell interkinesis DNA is synthetic, mitotic division is stopped, and cell can not normal growth.Chlorimuronethyl can be absorbed by the root of plant, stem, leaf, in plant materials, conducts up and down, is before selectivity bud, post-emergence herbicide, is mainly used in the farmlands such as soybean, prevents and kill off broadleaf weeds and some Cyperaceae and gramineous weeds.
Acetochlor, english common name acetochlor, chemical name 2-ethyl-6 methyl--N-ethoxyl methyl-alpha-chloro acetanilide N, structural formula as shown in Equation 2:
Acetochlor is interior absorption acetamide-group herbicides, is one of several herbicides of consumption maximum in agriculture production.Can be absorbed by weeds young shoot and young root, suppress weeds protein synthesis, and make weeds dead.For the various vegetables fields such as corn, cotton, soybean, peanut, rape, potato, sugarcane, sesame, Sunflower Receptacle and pulse family, Cruciferae, Solanaceae, composite family, Carrot family and orchard, prevent and kill off annual gramineous weed and part broadleaf weeds, Soybean Dodder is had to good preventive effect, invalid to perennial weeds, in soil, the lasting period can reach 2 months, and a dispenser can be controlled the whole crop growth phase and endanger without weeds.
A large amount of uses of chemical herbicide improve agricultural production efficiency greatly, but very large production and environmental problem have also been brought simultaneously, as long residual effect weedicide is on the rise to the poisoning of succession crop, affect agricultural planting adjustment of agricultural stracture, to agriculture production, cause heavy losses.Chlorimuronethyl has the advantages such as consumption is few, activity is high, herbicidal effect is good, firmly getting vast farmers likes, but chlorimuronethyl is release weedicide, in soil, the longevity of residure is longer, can reach 2-3, succession crop, as beet, potato, melon, jowar, rape, corn etc. all can produce poisoning in various degree, is made to plant-growth is slow, vegetative point is downright bad, branch and basal part of stem is aging, internode shortens, plant is downgraded, the ripening stage postpones, even cause crop dead.In using the Soybean Field of chlorimuronethyl, plant again Sunflower Receptacle, will cause the total crop failure of being injured of rear stubble Sunflower Receptacle big area.
The degraded of natural herbicide residue mainly completes by Soil Microorganism, but natural degradation is very slow.Therefore, screen targetedly high-effective microorganism bacterial strain, development microorganism renovation agent, the residual poisoning of degraded elimination farmland herbicide of accelerating soil lasting rudimental herbicide by artificial inoculation is a very necessary job and practicable approach.
Summary of the invention
The object of this invention is to provide a strain can efficient degradation weedicide chlorimuronethyl and the bacterium of acetochlor.
Bacterium provided by the present invention is fiber germ (Cellulosimicrobium sp.) LY1114, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on June 20th, 2013 and (is called for short CGMCC, address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City), deposit number is CGMCC No.7776.
Another object of the present invention is to provide a kind of microbial inoculum, and the activeconstituents of this microbial inoculum is described fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776.
Described microbial inoculum is following 1) or 2) described microbial inoculum:
1) for the microbial inoculum of degrading herbicide chlorimuronethyl and/or acetochlor;
2) microbial inoculum polluting for the residual weedicide chlorimuronethyl of rehabilitating soil and/or acetochlor.
Described microbial inoculum also can comprise carrier.Described carrier can be solid carrier or liquid vehicle.Described solid carrier is mineral material, vegetable material or macromolecular compound; Described mineral material is at least one in clay, talcum, kaolin, montmorillonite, white carbon, zeolite, silica and diatomite; Described vegetable material is at least one in Semen Maydis powder, bean powder and starch; Described macromolecular compound is polyvinyl alcohol and/or polyglycol.Described liquid vehicle is organic solvent, vegetables oil, mineral oil or water; Described organic solvent is decane and/or dodecane.In described microbial inoculum, described activeconstituents can exist with viable cell, the fermented liquid of viable cell, the form of the mixture of the filtrate of cell culture or cell and filtrate of being cultivated.The formulation of described composition can be multiple formulation, as liquor, emulsion, suspension agent, pulvis, granule, wettable powder or water dispersible granules.
As required, in described microbial inoculum, also can add tensio-active agent (as polysorbas20, tween 80 etc.), tackiness agent, stablizer (as antioxidant), pH adjusting agent etc.
The application of the microbial inoculum that described fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 or fiber germ (Cellulosimicrobium sp.) the LY1114CGMCC No.7776 of take are activeconstituents in degrading herbicide chlorimuronethyl and/or acetochlor also belongs to protection scope of the present invention.
The application of the microbial inoculum that described fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 or fiber germ (Cellulosimicrobium sp.) the LY1114CGMCC No.7776 of take are activeconstituents in the residual weedicide chlorimuronethyl of rehabilitating soil and/or acetochlor pollution also belongs to protection scope of the present invention.
A further object of the present invention is to provide the method for a kind of cultivation fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776, and the method comprises fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 in the step of cultivating for cultivating the substratum of fiber germ.
Another object of the present invention is to provide a kind of preparation method of take the microbial inoculum that fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 is activeconstituents, comprise the steps: described fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 as activeconstituents, to obtain described microbial inoculum.
The present invention is the farmland collection soil sample of polluting from being subject to for a long time residual weedicide chlorimuronethyl and acetochlor, and degrading herbicide chlorimuronethyl and acetochlor bacterial strain fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 that therefrom separated, screening obtains.Experiment showed, that this bacterial strain 7d in minimal medium reaches 89.11% to chlorimuronethyl (45mg/L) degradation rate; Acetochlor (45mg/L) degradation rate is reached to 32.08%.This shows, this bacterial strain energy efficient degradation weedicide chlorimuronethyl and acetochlor are having broad application prospects aspect the residual weedicide chlorimuronethyl of rehabilitating soil and acetochlor pollution.
preservation explanation
Strain name: fiber germ
Latin name: (Cellulosimicrobium sp.)
Strain number: LY1114
Preservation mechanism: China Committee for Culture Collection of Microorganisms's common micro-organisms center
Preservation mechanism is called for short: CGMCC
Address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City
Preservation date: on June 20th, 2013
The preservation center numbering of registering on the books: CGMCC No.7776
Embodiment
The experimental technique using in following embodiment if no special instructions, is ordinary method.
In following embodiment, material used, reagent etc., if no special instructions, all can obtain from commercial channels.
Enrichment culture liquid: K
2hPO
47g/L, KH
2pO
42g/L, MgSO
47H
2o0.1g/L, MnSO
40.05g/L, FeSO
40.05g/L, CaC1
20.003g/L, glucose 5g/L, is settled to 1L with distilled water, pH7.0; (chlorimuronethyl and acetochlor consumption are according to below embodiment 1 interpolation).
Isolation medium: be 2% to adding agar to its mass concentration in enrichment culture liquid.
Inorganic salt liquid substratum: NH
4cl1.0g/L, KH
2pO
40.5g/L, K
2hPO
41.5g/L, MgSO
40.2g/L, NaCl0.5g/L, is settled to 1L with distilled water, pH7.0.
Inorganic salt solid medium: be 2% to adding agar to its mass concentration in inorganic salt liquid substratum.
Beef-protein medium: extractum carnis 5g/L, peptone 10g/L, NaCl5g/L, agar 20g/L, is settled to 1L with distilled water, pH7.2.
Separation and the evaluation of embodiment 1, fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776
One, the separation of herbicide degradation bacterium LY1114
At 100mL containing adding 10g pedotheque (pick up from Shandong Province of China economize be subject to the farmland that Determination of Herbicide Acetochlor pollutes), 28 ℃, 180r/min shaking culture 7d enrichment chlorimuronethyl degradation bacteria in the enrichment culture liquid of chlorimuronethyl 20mg/L.After first round enrichment finishes, draw 10mL pregnant solution and be transferred in the 100mL enrichment culture liquid containing chlorimuronethyl 40mg/L, continuation cultivation 7d carries out second and takes turns enrichment.So continuously enrichment, cultivate 5 times, chlorimuronethyl concentration is followed successively by 20,40,60,80 and 100mg/L.
The screening of degradation bacteria adopts dull and stereotyped transparent circle method.Add inorganic salt solid medium to make flat board chlorimuronethyl, final concentration is 1000mg/L.Flat board is divided into Si Ge district, and the different samples of every district mark (pregnant solution) numbering, gets pregnant solution 15 μ L dibblings to numbering area, 28 ℃ of cultivation, observation.The sample (pregnant solution) that has transparent circle to occur is become to 10 with sterilized water doubling dilution
-1, 10
-2, 10
-3, 10
-4, 10
-5gradient, gets 10
-3, 10
-4, 10
-5each 100 μ L of gradient are to the inorganic salt solid medium of above-mentioned chloride sulfometuron-methyl 1000mg/L, and 3 repetitions of each gradient, are coated with evenly.28 ℃ of cultivations, observe, until dull and stereotyped, above occur that after single bacterium colony, picking has single bacterium colony of transparent circle to be forwarded on beef-protein medium, 25 ℃ of cultivations, purifying, standby.
Through a large amount of enrichment culture, being separated to can be in bacterial strain more than 60 strain containing forming transparent circle on the inorganic salt plate culture medium of chlorimuronethyl 1000mg/L.Further, after screening, obtain a bacterial strain that is numbered LY1114, called after herbicide degradation bacterium LY1114, this bacterial strain can, under pure culture condition, degrade 89.11% by the chlorimuronethyl of starting point concentration 45mg/L in 7d; And the acetochlor of can also degrading, 7d is degraded to 32.08% by the acetochlor of starting point concentration 45mg/L.
Two, the evaluation of herbicide degradation bacterium LY1114
The herbicide degradation bacterium LY1114 separated from the following aspects authentication step one and purifying obtains:
1, Morphological Identification
Will be in logarithmic phase, and bacterium colony size is stable, above-mentioned steps one herbicide degradation bacterium LY1114 separated and that purifying obtains carries out single bacterium colony state observation, mainly comprises size, color, transparency, wettability, bacterium colony condition of surface (whether smooth, projection, fold, depression etc.), the colony edge state (whether neat, irregular, radial etc.) of bacterium colony.
For the described herbicide degradation bacterium LY1114 in logarithmic phase, after smear staining, adopt the form of observation by light microscope thalline.
Result shows, the herbicide degradation bacterium LY1114 bacterium colony circular protrusion that above-mentioned steps one is separated and purifying obtains, faint yellow, smooth surface is moistening, neat in edge; Thalline rod-short, Gram-positive.
2, analysis of physio biochemical characteristics
With reference to < < common bacteria system identification handbook > > (eastern elegant pearl, Cai Miaoying. common bacteria system identification handbook. Beijing: Science Press, 2011.) and < < Microbiology Experiment > > (Shen Ping, Fan Xiurong, Li Guangwu. Microbiology Experiment (third edition). Beijing: Higher Education Publishing House, 1999.) measure the physiological and biochemical property of above-mentioned herbicide degradation bacterium LY1114.
The physiological and biochemical property measurement result of described herbicide degradation bacterium LY1114 is as shown in table 1:
The physiological and biochemical property of table 1 herbicide degradation bacterium LY1114
Note: "+" represents positive, "-" represents negative.
3,16s rDNA sequence homology analysis
Ordinary method is cultivated the herbicide degradation bacterium LY1114 that above-mentioned steps one separation and purification obtains, extract total DNA of bacterial strain as gene amplification template, with bacterium 16s rDNA universal primer, 27f:5 '-AGAGTTTGATCCTGGCTCAG-3 ', 1492r:5 '-TACCTTGTTACGACTT-3 ' carries out PCR reaction.Reaction system adopts Shanghai biotechnology company limited pcr amplification test kit.Response procedures is: 95 ℃ of sex change 30s, 55 ℃ of annealing 1min, 72 ℃ of extensions 2min, totally 30 circulations.DNA sequencing is completed by Beijing San Bo polygala root biotech company, sequence assembly and similarity analysis are used DNAStar software to complete, and gene comparison completes online by American National biotechnology information center ncbi database (http://www.ncbi.nlm.nih.gov) and EzTaxon.
Pcr gene amplification obtains the about 1.4kb of 16S rDNA gene fragment of herbicide degradation bacterium LY1114, measure after sequence with NCBI and Ez Taxon database in published 16S rDNA sequence carry out online sequence analysis, result shows LY1114 and funk fiber germ Cellulosimicrobium funkei ATCC BAA-886
tthe homology of (GenBank accession number AY501364) is the highest, reaches 99.709%.
The 16s rDNA sequence of herbicide degradation bacterium LY1114 refers to sequence 1 in sequence table.
In view of above-mentioned form, analysis of physio biochemical characteristics and 16s rDNA sequence homology analysis result, the herbicide degradation bacterium LY1114 that step 1 separation and purification is obtained is accredited as bacterium territory actinomycete group micrococcaceae (Micrococcineneae) fiber germ and belongs to (Cellulosimicrobium sp.).This herbicide degradation bacterium LY1114 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on June 20th, 2013 and (is called for short CGMCC, address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City), deposit number is CGMCC No.7776.
Embodiment 2, fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 degrading herbicide chlorimuronethyl and the quantitative assay of acetochlor ability
One, chlorimuronethyl bioassay standard curve plotting
With methyl alcohol, chlorimuron ethyl (purchased from Fluka company) is mixed with to series concentration 10,20,40,60,80,100mg/L standardized solution, adopts high performance liquid chromatography (HPLC) to measure the peak area of different concns chlorimuron ethyl, repeat for 3 times.The concentration of chlorimuronethyl of take is X-coordinate, and peak area is ordinate zou, draws chlorimuronethyl typical curve.
Testing conditions is as follows:
Detection system: Agilent1100Series.Chromatographic column: C18Diamosil
tMreversed-phase column, 250mm * 4.6mm, particle diameter 5 μ m.Chromatographic condition: moving phase: acetonitrile: methyl alcohol: water: glacial acetic acid=45:15:40:0.1(v/v); Detect wavelength, 236nm; Flow velocity, 1.0mL/min; Sampling volume, 10 μ L; 30 ℃ of column temperatures.
Measurement result is as shown in table 2:
Table 2 different concns chlorimuronethyl HPLC measures peak area
According to table 2 data, obtain chlorimuronethyl bioassay standard curve: y=33.717x-20.032 (R
2=0.9950).Wherein, y is peak area, and x is chlorimuronethyl concentration.
Two, acetochlor bioassay standard curve plotting
With methyl alcohol, acetochlor standard substance (purchased from Fluka company) are mixed with to series concentration 10,20,40,60,80,100mg/L standardized solution, adopt high performance liquid chromatography (HPLC) to measure the peak area of different concns acetochlor standard substance, repeat for 3 times.The concentration of acetochlor of take is X-coordinate, and peak area is ordinate zou, draws acetochlor typical curve.
Testing conditions is as follows:
Detection system: Agilent1100Series.Chromatographic column: C18Diamosil
tMreversed-phase column, 250mm * 4.6mm, particle diameter 5 μ m.Chromatographic condition: moving phase: methyl alcohol: water=80:20(v/v), water is adjusted to pH=3 with Glacial acetic acid; Detect wavelength, 215nm; Flow velocity, 1.0mL/min; Sampling volume, 20 μ L; 30 ℃ of column temperatures.
Measurement result is as shown in table 3:
Table 3 different concns acetochlor HPLC measures peak area
According to table 3 data, obtain acetochlor bioassay standard curve: y=51.44x+1615 (R
2=0.999).Wherein, y is peak area, and x is acetochlor concentration.
Three, fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 degraded chlorimuronethyl ability quantitative assay
In test tube, accurately add 5mL to contain the minimal medium (being the substratum that about 50mg/L obtains to adding chlorimuronethyl to make the concentration of chlorimuronethyl in inorganic salt liquid substratum) of the about 50mg/L of chlorimuronethyl, then add fiber germ (Cellulosimicrobium sp.) the LY1114CGMCC No.7776 bacterium liquid (1 * 10 of OD600nm=1.0
9cfu/ml) 0.2mL, 28 ℃, 180r/min are cultivated 7 days, get 4mL degradation solution to 50mL centrifuge tube, add 4mL methylene dichloride, vibration 2min, standing 10min, adds a little anhydrous sodium sulphate.Accurately drawing 500 μ L organic phases is transferred in 1.5mL EP centrifuge tube, on Nitrogen evaporator, dry up, add 500 μ L methyl alcohol (chromatographic grade), on ultrasonic washing instrument, dissolve 10min, with liquid spectrum strainer, filter and be collected in sample bottle, according to above-mentioned HPLC method, measure chlorimuronethyl.Meanwhile, using do not inoculate fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 the above-mentioned minimal medium containing the about 50mg/L of chlorimuronethyl as blank, measure according to the method described above the concentration of chlorimuronethyl.
Chlorimuronethyl degradation rate: X=(1-A/B) * 100%, in formula, X is degradation rate (%), and A connects chlorimuronethyl concentration residual in bacterium treatment solution, and B is not for connecing chlorimuronethyl concentration residual in bacterium blank treatment solution.3 repetitions are established in experiment, repeat each at every turn and process 1 test tube of inoculation.
Result shows, chlorimuronethyl starting point concentration is 45.09mg/L, after 7 days described fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 that the degradation rate of chlorimuronethyl is reached to 89.11%(is as shown in table 4).This result shows, fiber germ provided by the present invention (Cellulosimicrobium sp.) LY1114CGMCC No.7776 can efficient degradation chlorimuronethyl.
Table 4 fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 degraded chlorimuronethyl effect
Four, fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 degraded acetochlor ability quantitative assay
At 50mL, access fiber germ (Cellulosimicrobium sp.) the LY1114CGMCC No.7776 bacterium liquid (1 * 10 of OD600nm=1.0 in containing the triangular flask (volume is 250ml) of the minimal medium of the about 50mg/L of acetochlor
9cfu/ml) 2mL, 30 ℃, 200r/min shaking table is cultivated 7 days, measures Residual Determination of Acetochlor.Method is: get 3mL degradation solution to 50mL centrifuge tube, the centrifugal 5min of 8000r/min collects supernatant liquor, add 3mL methylene dichloride, thermal agitation 5min, standing 10min, treat water and organic phase layering, add a small amount of anhydrous sodium sulphate to dewater to organic phase, accurately draw 800 μ L organic phases in the centrifuge tube of 1.5mL, with Nitrogen evaporator, dry up, add 400 μ L methyl alcohol, ultrasonic 10min in ultrasonic cleaner, with the organic phase aculeus type filter of 0.22 μ m, be filtered to liquid chromatography sample bottle, according to above-mentioned HPLC method, measure acetochlor.Meanwhile, using the above-mentioned minimal medium containing the about 50mg/L of acetochlor of (Cellulosimicrobium sp.) the LY1114CGMCC No.7776 that there is no incoming fiber germ as blank, measure according to the method described above the concentration of acetochlor.Acetochlor degradation rate: X=(1-A/B) * 100%, in formula, X is degradation rate (%), and A connects acetochlor concentration residual in bacterium treatment solution, and B is not for connecing acetochlor concentration residual in bacterium blank treatment solution.3 repetitions are established in experiment, repeat each at every turn and process 1 triangular flask of inoculation.
Result shows, acetochlor starting point concentration is 44.78mg/L, after 7 days described fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 that the degradation rate of acetochlor is reached to 32.08%(is as shown in table 5).This result shows, fiber germ provided by the present invention (Cellulosimicrobium sp.) LY1114CGMCC No.7776 can efficient degradation acetochlor.
Table 5 fiber germ (Cellulosimicrobium sp.) LY1114CGMCC No.7776 degraded acetochlor effect
Sequence table
Claims (7)
- Fiber germ ( cellulosimicrobium sp.) LY1114, the deposit number at Qi China Committee for Culture Collection of Microorganisms common micro-organisms center is CGMCC No.7776.
- 2. a microbial inoculum, is characterized in that: the activeconstituents of described microbial inoculum be fiber germ claimed in claim 1 ( cellulosimicrobium sp.) LY1114.
- 3. microbial inoculum according to claim 2, is characterized in that: described microbial inoculum is following 1) or 2) described microbial inoculum:1) for the microbial inoculum of degrade chlorimuronethyl and/or acetochlor;2) microbial inoculum polluting for rehabilitating soil chlorimuronethyl and/or acetochlor.
- Fiber germ claimed in claim 1 ( cellulosimicrobium sp.) application in degraded chlorimuronethyl and/or acetochlor of microbial inoculum described in LY1114 or claim 2 or 3.
- Fiber germ claimed in claim 1 ( cellulosimicrobium sp.) application in rehabilitating soil chlorimuronethyl and/or acetochlor pollute of microbial inoculum described in LY1114 or claim 2 or 3.
- 6. cultivate fiber germ claimed in claim 1 ( cellulosimicrobium sp.) method of LY1114, comprise by described fiber germ ( cellulosimicrobium sp.) LY1114 is in the step of cultivating for cultivating the substratum of fiber germ.
- 7. the preparation method of microbial inoculum described in claim 2 or 3, comprise the steps: by fiber germ claimed in claim 1 ( cellulosimicrobium sp.) LY1114 is as activeconstituents, obtains described microbial inoculum.
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| CN103820360B (en) * | 2014-01-16 | 2016-04-20 | 南京农业大学 | One strain can effectively be degraded the degradation bacteria of propyzamide and application thereof |
| CN104498389B (en) * | 2014-11-26 | 2017-01-18 | 中国农业科学院农业资源与农业区划研究所 | Bacterium for degrading herbicide namely Pursuit (imazethapyr-ammonium) and acetochlor and application of bacterium |
| CN106520613B (en) * | 2014-11-26 | 2019-04-16 | 中国农业科学院农业资源与农业区划研究所 | The bacterium that one plant of degrading herbicide Acetochlor and weeds are burnt |
| CN104480043B (en) * | 2014-12-12 | 2017-04-12 | 中国农业科学院农业资源与农业区划研究所 | Rhodococcus sp. capable of degrading four herbicides and application thereof |
| CN112662598B (en) * | 2021-01-27 | 2023-11-21 | 新疆石大科技股份有限公司 | Fender fiber microzyme, microbial inoculum comprising Fender fiber microzyme, preparation method and application |
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