CN101347177A - Method for preparing Bacillus Subtilis fermented mixed feed for birds - Google Patents
Method for preparing Bacillus Subtilis fermented mixed feed for birds Download PDFInfo
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- CN101347177A CN101347177A CNA2008100541473A CN200810054147A CN101347177A CN 101347177 A CN101347177 A CN 101347177A CN A2008100541473 A CNA2008100541473 A CN A2008100541473A CN 200810054147 A CN200810054147 A CN 200810054147A CN 101347177 A CN101347177 A CN 101347177A
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- bacillus subtilis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a preparation method of a bacillus subtilis fermented mixed feed for poultry. The method comprises the following steps: (1) the bacillus subtilis is cultivated to become a primary seed by broth culture; (2) the primary seed liquid is in amplification culture by the broth culture to become a secondary seed; (3) the secondary seed liquid is in fermentation culture by the broth culture to become a tertiary seed; (4) rice bran, wheat bran and crushed corn are mixed, and then are evenly mixed with the tertiary seed liquid, the mixture is further fermented and cultivated to obtain a first fermented feed; (5) the first fermented feed is mixed with bean cakes, the wheat bran, fish meal, bone meal, conch powder and common salt to obtain the bacillus subtilis fermented mixed feed for the poultry. The method of the invention has the advantages of improving the nutritional components of a coarse feed and causing the vegetable fiber to be transformed into small molecules which are easy to be absorbed by the poultry. By applying the mixed feed prepared by the method to the poultry, the mixed feed can promote the growth of the poultry without using antibiotics and hormone.
Description
Technical field
The present invention relates to a kind of preparation method of microbial fermentation mixed fodder, particularly relate to a kind of preparation method of bird fermentation of bacillus subtilis mixed fodder.
Background technology
Bird is raised feeds utilized, to consider that on the one hand the nutritional labeling in the feed improves the feed cost performance, to consider also that in addition can feed itself improve the resistance of bird to pathogenic microorganisms, common feed usually is to wherein adding " three elements ", that is: antibiotic, hormone and vitamin, but the abuse of " three elements " can feed through to the quality as the meat and the egg of human food's birds, the mankind are caused potential harm, therefore, need a kind of nutritional labeling that can improve roughage badly, make string transform the feed that the easy absorbing material of little molecule bird can improve the bird resistance again.
Bacillus subtilis sporadicly is present in the animal and bird intestines microbiologic population.Compare with probios such as lactic acid bacteria commonly used, bacillus is an aerobic bacteria, produces gemma under certain condition.Thereby bacillus has high temperature resistant, acid and alkali-resistance, characteristics such as withstand voltage, can tolerate the influence of pellet process and sour environment and the cholate of enteron aisle epimere and the destruction of digestive juice in the stomach, after entering the enteron aisle back segment, bring back to life rapidly, and its resurrection rate is higher at enteron aisle.Bacillus is kept the enteron aisle anaerobic environment at a large amount of oxygen of enteron aisle internal consumption, suppresses the growth and breeding of aerobic pathogenic bacteria, keeps host's intestinal microecology balance.
Summary of the invention
The purpose of this invention is to provide a kind of preparation method that can promote the bird fermentation of bacillus subtilis mixed fodder of bird growth.
Technical scheme of the present invention is summarized as follows:
A kind of preparation method of bird fermentation of bacillus subtilis mixed fodder comprises the steps:
(1) is seed culture medium with broth bouillon, under 30～45 ℃ of aerobic conditions, cultivated bacillus subtilis 8～30 hours, become first order seed;
(2) primary seed solution is seeded in enlarges cultivation in the seeding tank, the employing broth bouillon is a secondary seed medium, cultivates 8～30 hours under 30～45 ℃ of aerobic conditions, becomes secondary seed;
(3) secondary seed solution is seeded in carries out fermented and cultured in the fermentation tank, the employing broth bouillon is a fermentation medium, under 30～45 ℃ of aerobic conditions, adopts regularly or continuous feeding batch fermentation mode was cultivated 8～36 hours, becomes three grades of seeds;
(4) rice bran, wheat bran, broken corn are mixed the back and evenly mix with three grades of seed liquor, fermented and cultured is 36～60 hours again, makes first fermented feed;
(5) fermented feed and soya-bean cake, wheat bran, fish meal, bone meal, oyster shell whiting, salt are mixed into bird fermentation of bacillus subtilis mixed fodder for the first time.
The mass ratio of rice bran, wheat bran, broken corn is 1: 1～5: 1～5 described in the described step (4), and the mass ratio that described rice bran, wheat bran, broken corn mix back and three grades of seed liquor is 100: 0.2～1.
The mass ratio of described rice bran, wheat bran, broken corn is 1: 1: 1.
Fermented incubation time is 48 hours described in the described step (4).
The mass ratio of first fermented feed and soya-bean cake, wheat bran, fish meal, bone meal, oyster shell whiting, salt is 50～70: 10～20 described in the described step (5): 5～10: 5～15: 0.2～1: 0.2～1: 0.2～1.
The mass ratio of described first fermented feed and soya-bean cake, wheat bran, fish meal, bone meal, oyster shell whiting, salt 65: 17: 7: 9.5: 0.63: 0.45: 0.37.
Advantage of the present invention:
Method of the present invention can be improved the nutritional labeling of roughage, makes string be converted into the material that little molecule bird easily absorbs, and the mixed fodder for preparing with method of the present invention can not use antibiotic and hormone, and can promote the growth of bird when being applied to bird.
Promote that bird efficiently increases weight, method of the present invention has that production technology is simple, the low cost and other advantages of product.
The specific embodiment
The present invention is further illustrated below in conjunction with specific embodiment.
Embodiment 1
A kind of preparation method of bird fermentation of bacillus subtilis mixed fodder comprises the steps:
(1) is seed culture medium with broth bouillon, under 40 ℃ of aerobic conditions, cultivated bacillus subtilis 18 hours, become first order seed;
(2) primary seed solution is seeded in enlarges cultivation in the seeding tank, the employing broth bouillon is a secondary seed medium, cultivates 18 hours under 40 ℃ of aerobic conditions, becomes secondary seed;
(3) secondary seed solution is seeded in carries out fermented and cultured in the fermentation tank, the employing broth bouillon is a fermentation medium, under 40 ℃ of aerobic conditions, adopts regularly or continuous feeding batch fermentation mode was cultivated 18 hours, becomes three grades of seeds;
(4) be that 1: 1: 1 rice bran, wheat bran, broken corn mixes the back and evenly mix with three grades of seed liquor with mass ratio, the mass ratio that described rice bran, wheat bran, broken corn mix back and three grades of seed liquor is 100: 0.5, fermented and cultured is 48 hours again, makes first fermented feed;
(5) fermented feed and soya-bean cake, wheat bran, fish meal, bone meal, oyster shell whiting, salt are 65: 17: 7 in mass ratio for the first time: 9.5: 0.63: 0.45: 0.37 ratio was mixed into bird fermentation of bacillus subtilis mixed fodder.
Embodiment 2
A kind of preparation method of bird fermentation of bacillus subtilis mixed fodder, its feature comprises the steps:
(1) is seed culture medium with broth bouillon, under 30 ℃ of aerobic conditions, cultivated bacillus subtilis 30 hours, become first order seed;
(2) primary seed solution is seeded in enlarges cultivation in the seeding tank, the employing broth bouillon is a secondary seed medium, cultivates 30 hours under 30 ℃ of aerobic conditions, becomes secondary seed;
(3) secondary seed solution is seeded in carries out fermented and cultured in the fermentation tank, the employing broth bouillon is a fermentation medium, under 30 ℃ of aerobic conditions, adopts regularly or continuous feeding batch fermentation mode was cultivated 36 hours, becomes three grades of seeds;
(4) be that 1: 5: 1 rice bran, wheat bran, broken corn mixes the back and evenly mix with three grades of seed liquor with mass ratio, the mass ratio that described rice bran, wheat bran, broken corn mix back and three grades of seed liquor is 100: 0.2, fermented and cultured is 50 hours again, makes first fermented feed;
(5) fermented feed and soya-bean cake, wheat bran, fish meal, bone meal, oyster shell whiting, salt are 60: 10: 10 by mass ratio for the first time: 10: 0.8: 0.2: 0.2 was mixed into bird fermentation of bacillus subtilis mixed fodder.
Embodiment 3
A kind of preparation method of bird fermentation of bacillus subtilis mixed fodder, its feature comprises the steps:
(1) is seed culture medium with broth bouillon, under 45 ℃ of aerobic conditions, cultivated bacillus subtilis 8 hours, become first order seed;
(2) primary seed solution is seeded in enlarges cultivation in the seeding tank, the employing broth bouillon is a secondary seed medium, cultivates 8 hours under 45 ℃ of aerobic conditions, becomes secondary seed;
(3) secondary seed solution is seeded in carries out fermented and cultured in the fermentation tank, the employing broth bouillon is a fermentation medium, under 45 ℃ of aerobic conditions, adopts regularly or continuous feeding batch fermentation mode was cultivated 8 hours, becomes three grades of seeds;
(4) be that 1: 1～5: 1～5 rice bran, wheat bran, broken corn mix the back and evenly mix with three grades of seed liquor with mass ratio, the mass ratio that described rice bran, wheat bran, broken corn mix back and three grades of seed liquor is 100: 1, fermented and cultured is 36 hours again, makes first fermented feed;
(5) fermented feed and soya-bean cake, wheat bran, fish meal, bone meal, oyster shell whiting, salt are 50: 20: 8 by mass ratio for the first time: 5: 0.2: 1: 1 was mixed into bird fermentation of bacillus subtilis mixed fodder.
Embodiment 4
A kind of preparation method of bird fermentation of bacillus subtilis mixed fodder, its feature comprises the steps:
(1) is seed culture medium with broth bouillon, under 36 ℃ of aerobic conditions, cultivated bacillus subtilis 24 hours, become first order seed;
(2) primary seed solution is seeded in enlarges cultivation in the seeding tank, the employing broth bouillon is a secondary seed medium, cultivates 24 hours under 36 ℃ of aerobic conditions, becomes secondary seed;
(3) secondary seed solution is seeded in carries out fermented and cultured in the fermentation tank, the employing broth bouillon is a fermentation medium, under 36 ℃ of aerobic conditions, adopts regularly or continuous feeding batch fermentation mode was cultivated 24 hours, becomes three grades of seeds;
(4) be that 1: 1: 5 rice bran, wheat bran, broken corn mixes the back and evenly mix with three grades of seed liquor with mass ratio, the mass ratio that described rice bran, wheat bran, broken corn mix back and three grades of seed liquor is 100: 0.8, fermented and cultured is 60 hours again, makes first fermented feed;
(5) fermented feed and soya-bean cake, wheat bran, fish meal, bone meal, oyster shell whiting, salt are 70: 15: 5 by mass ratio for the first time: 15: 1: 0.8: 0.8 was mixed into bird fermentation of bacillus subtilis mixed fodder.
Embodiment 5
A kind of preparation method of bird fermentation of bacillus subtilis mixed fodder, its feature comprises the steps:
(1) is seed culture medium with broth bouillon, under 40 ℃ of aerobic conditions, cultivated bacillus subtilis 12 hours, become first order seed;
(2) primary seed solution is seeded in enlarges cultivation in the seeding tank, the employing broth bouillon is a secondary seed medium, cultivates 12 hours under 40 ℃ of aerobic conditions, becomes secondary seed;
(3) secondary seed solution is seeded in carries out fermented and cultured in the fermentation tank, the employing broth bouillon is a fermentation medium, under 40 ℃ of aerobic conditions, adopts regularly or continuous feeding batch fermentation mode was cultivated 12 hours, becomes three grades of seeds;
(4) be that 1: 2: 3 rice bran, wheat bran, broken corn mixes the back and evenly mix with three grades of seed liquor with mass ratio, the mass ratio that described rice bran, wheat bran, broken corn mix back and three grades of seed liquor is 100: 0.8, fermented and cultured is 48 hours again, makes first fermented feed;
(5) fermented feed and soya-bean cake, wheat bran, fish meal, bone meal, oyster shell whiting, salt are 60: 18: 7 by mass ratio for the first time: 10: 0.6: 0.8: 0.9 was mixed into bird fermentation of bacillus subtilis mixed fodder.
A kind of bird fermentation of bacillus subtilis mixed fodder of the present invention can produce protease, amylase, lipase and several amino acids, promotes the bird growth.The feed of method system pavilion of the present invention can reduce the incidence of young fowl bacterial diarrhea, improves survival rate; Reduce production costs, improve the food conversion utilization rate; Improve body's immunity.The feed that adds method preparation of the present invention in meat chick daily ration can make the average daily ingestion amount of meat chick, daily gain and feed conversion rate all be better than the blank group, make an addition to the feed of a small amount of method of the present invention preparation in the chicken feed or view and admire in the feed of bird, the resistance against diseases that can improve chicken or view and admire bird, the disease that the checken pest of protecting from infection is sick and other microorganisms cause improves the survival rate of chicken.Make that harmful microbe bacterium colony number such as salmonella descends greatly in chicken or the egg, thereby improved the edible safety rank of poultry egg food.
Experimental example
1. the feed of method preparation of the present invention is to the treatment of simple property chicken diarrhea
Certain chicken house is raised 2000 of laying hens, once in 30 age in days generation gumboro diseases (IBD), with hyper-immune serum and antibiotic therapy, after this continue to have 50% left and right sides chicken diarrhea among the chicken group, and cause the death rate of average approximately every day 0.1%. go to a doctor until 54 ages in days, once used oral medications such as furazolidone, Diverdine, terramycin therebetween, logotype one all courses of disease take a turn for the better, but drug withdrawal is recurrence again soon, do not have fixing pathogenic former through check, 15 parts on excrement smear, average Gram-positive bacillus accounts for 13.57%, and great majority are gram-Negative bacillus in the visual field.
By the beverage of the method for the present invention preparation of feeding, throw clothes with every chicken viable bacteria dosage every day 0.25 hundred million, full group recovers after 5 days, feed intake by every day every 100 gram increase to 110 grams.16 parts on excrement smear, average Gram-positive bacillus accounts for 97.13%, and some smear almost is the purebred state of bifurcated lactobacillus.
2. to the control of salmonellosis of chicken
Certain meat chicken house, when advancing young bird, 2000 chickens are divided into two groups, under the equal conditions, one group of feed of throwing clothes method preparation of the present invention with every chicken viable bacteria dosage every days 5,000,000 wherein, none salmonella case and chicken diarrhea case between 2 ages in week, control group has 0.045% salmonellosis chicken death then average every day, and control group gives every chicken viable bacteria dosage every days 0.2 hundred million from drinking-water after 2 ages in week, no new case occurs after 5 days, and death stops.
3. to the treatment of suprainfection
Four meat chicken houses in certain township (each 2000 of the numbers of animals raised), in feed, add 0.04% furazolidone for the prevention dysentery characterized by white mucous stool when advancing young bird, increased on this basis after age in 1 week and feed 0.5% terramycin, add the chicken treasured again after 2 ages in week, after this also once between or use penicillin drinking-water.After 4 ages in week, diarrhea almost takes place in the chicken group of these four chicken houses simultaneously, and the death rate of 1%-2% is arranged every day.It is hemorrhage to cut open the visible intestinal mucosa hyperemia of inspection, the chicken that has in the thoracic cavity or the abdominal cavity mould lawn of diameter 3,20mm size is arranged, do not have other fixedly cause of disease, be suprainfection through comprehensive diagnos.Visible down a large amount of fungal spores of 10 parts of fecal smears, mirror and saccharomycete check behind the Gram that Gram-positive bacillus accounts for 3.2%.
Throw clothes with the feed of method of the present invention preparation (0.5 hundred million viable bacterias/only, day), recovery from illness after 4 days, death stops, and it is normal that excrement smear enteric bacteria recovers.
Claims (6)
1. the preparation method of a bird fermentation of bacillus subtilis mixed fodder, its feature comprises the steps:
(1) is seed culture medium with broth bouillon, under 30～45 ℃ of aerobic conditions, cultivated bacillus subtilis 8～30 hours, become first order seed;
(2) primary seed solution is seeded in enlarges cultivation in the seeding tank, the employing broth bouillon is a secondary seed medium, cultivates 8～30 hours under 30～45 ℃ of aerobic conditions, becomes secondary seed;
(3) secondary seed solution is seeded in carries out fermented and cultured in the fermentation tank, the employing broth bouillon is a fermentation medium, under 30～45 ℃ of aerobic conditions, adopts regularly or continuous feeding batch fermentation mode was cultivated 8～36 hours, becomes three grades of seeds;
(4) rice bran, wheat bran, broken corn are mixed the back and evenly mix with three grades of seed liquor, fermented and cultured is 36～60 hours again, makes first fermented feed;
(5) fermented feed and soya-bean cake, wheat bran, fish meal, bone meal, oyster shell whiting, salt are mixed into bird fermentation of bacillus subtilis mixed fodder for the first time.
2. the preparation method of a kind of bird fermentation of bacillus subtilis mixed fodder according to claim 1, the mass ratio that it is characterized in that rice bran, wheat bran, broken corn described in the described step (4) is 1: 1～5: 1～5, and the mass ratio that described rice bran, wheat bran, broken corn mix back and three grades of seed liquor is 100: 0.2～1.
3. the preparation method of a kind of bird fermentation of bacillus subtilis mixed fodder according to claim 2, the mass ratio that it is characterized in that described rice bran, wheat bran, broken corn is 1: 1: 1.
4. the preparation method of a kind of bird fermentation of bacillus subtilis mixed fodder according to claim 1 is characterized in that fermented incubation time is 48 hours described in the described step (4).
5. the preparation method of a kind of bird fermentation of bacillus subtilis mixed fodder according to claim 1 is characterized in that the mass ratio of first fermented feed described in the described step (5) and soya-bean cake, wheat bran, fish meal, bone meal, oyster shell whiting, salt is 50～70: 10～20: 5～10: 5～15: 0.2～1: 0.2～1: 0.2～1.
6. the preparation method of a kind of bird fermentation of bacillus subtilis mixed fodder according to claim 5 is characterized in that the mass ratio 65: 17: 7 of described first fermented feed and soya-bean cake, wheat bran, fish meal, bone meal, oyster shell whiting, salt: 9.5: 0.63: 0.45: 0.37.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2008100541473A CN101347177A (en) | 2008-08-15 | 2008-08-15 | Method for preparing Bacillus Subtilis fermented mixed feed for birds |
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| CNA2008100541473A CN101347177A (en) | 2008-08-15 | 2008-08-15 | Method for preparing Bacillus Subtilis fermented mixed feed for birds |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101773209A (en) * | 2010-02-10 | 2010-07-14 | 浙江科盛饲料股份有限公司 | Compound feed for shellfish |
| CN102178092A (en) * | 2011-04-22 | 2011-09-14 | 河南宏翔生物科技有限公司 | Method for preparing high-moisture fermented granular feed for poultry |
| CN103181478A (en) * | 2011-12-27 | 2013-07-03 | 北京资源亚太饲料科技有限公司 | Microecological preparation for poultry and its preparation method |
| CN104222522A (en) * | 2014-09-26 | 2014-12-24 | 河南科技大学 | Chicken feed capable of preventing pullorum of black-bone chicken and preparation method of chicken feed |
-
2008
- 2008-08-15 CN CNA2008100541473A patent/CN101347177A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101773209A (en) * | 2010-02-10 | 2010-07-14 | 浙江科盛饲料股份有限公司 | Compound feed for shellfish |
| CN102178092A (en) * | 2011-04-22 | 2011-09-14 | 河南宏翔生物科技有限公司 | Method for preparing high-moisture fermented granular feed for poultry |
| CN102178092B (en) * | 2011-04-22 | 2012-10-24 | 河南宏翔生物科技有限公司 | Method for preparing high-moisture fermented granular feed for poultry |
| CN103181478A (en) * | 2011-12-27 | 2013-07-03 | 北京资源亚太饲料科技有限公司 | Microecological preparation for poultry and its preparation method |
| CN104222522A (en) * | 2014-09-26 | 2014-12-24 | 河南科技大学 | Chicken feed capable of preventing pullorum of black-bone chicken and preparation method of chicken feed |
| CN104222522B (en) * | 2014-09-26 | 2017-08-08 | 河南科技大学 | It is a kind of to prevent chicken feed of bamboo silk white diarrhea and preparation method thereof |
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Application publication date: 20090121 |