CN103564269A - Microbial feed additive for reducing diarrhea of piglet, and preparation method of microbial feed additive - Google Patents
Microbial feed additive for reducing diarrhea of piglet, and preparation method of microbial feed additive Download PDFInfo
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Abstract
The invention discloses a microbial feed additive for reducing diarrhea of a piglet, and a preparation method of the microbial feed additive. The method particularly comprises the steps that lactobacillus plantarum and bifidobacterium adolescentis are fermented synchronously, freeze-dried and mixed with skimmed milk powder; then a finished product is obtained. The microbial feed additive can effectively restrain invasion of cultures such as escherichia coli and salmonella, and establishes a biologic barrier in an intestinal tract, so that ingress of toxic and hazardous substances can be reduced; ecological balance of the intestinal tract can be maintained effectively; an organism is prompted to generate an antibacterial active substance; an immunologic function of the piglet is improved; the disease resistance of the piglet is improved; a diarrhea rate of the piglet is reduced significantly; a daily weight gain is increased; the appearance of the piglet can be improved. The preparation method is simple and easy to operate, shorter in preparation period and low in production cost, and is suitable for large-scale production, preparation and application.
Description
Technical field
The present invention relates to additive for microbe feedstuff, particularly a kind ofly can reduce microbiological feed of grice diarrhoea and preparation method thereof.
Background technology
In recent years, along with the intensive production of height of herding industry, the generation of livestock and poultry is also risen increasingly, and the death rate causing due to disease increases year by year, to animal husbandry, causes great economic loss.Diarrhoea is to affect piglet survival ratio and one of principal element of growing, because piglet is at weaning stage, its digestive function ateliosis, cause its digestive ferment hyposecretion, cannot adapt to feed, thereby cause the piglet appetite of searching for food to decline, indigestion, its premunition and immunity degradation, cause the symptoms such as diarrhoea simultaneously.Grice diarrhoea, as can not get suitable processing, can make piglet survival ratio decline or grow slowly, to pig industry, brings huge economic loss.
Traditional prevention and treatment measure are to use antibiotic, as the antibiotics such as Enrofloxacin, terramycin, kanamycins, gentamicin are controlled.
As disclosed a kind of additive for fodder that reduces grice diarrhoea and promote growth in patent CN 102630856A, this feed is comprised of basal diet and these two parts of quinocetone, consisting of of basal diet wherein: wheat 50.00-55.00%, corn 17.175-20%, dregs of beans 14.06-14.5%, wheat bran 9.00-10.00%, salt 0.30-0.35%, calcium monohydrogen phosphate 0.64-0.72%, methionine 0.50-0.06%, stone flour 1.05-1.09%, lysine 0.70-0.75%, SV220.12-0.15%, phytase 0.015-0.02%, wheat enzyme 0.02-0.025%, M-S20.15-0.25%, quinocetone additive part: add 0.002-0.008 kilogram by every hundred kilograms of described basal diets.But use antibiotic easily to lead to grave consequences, as the generation of drug-fast bacteria, the antibiotic problem such as residual in livestock products, not only cannot solve the diarrhoea problem of piglet, also may cause serious harm to human body.
People also recognize the harm of abuse of antibiotics or other harmful additives gradually at present, start to attempt adding natural additive to reduce the appearance of grice diarrhoea situation in the feed of piglet.
A kind of diarrhea resistant composite Chinese herbal medicine feed additive for pigling is disclosed in patent CN101427731B, the actual conditions of producing according to Pig Industry, carry out repetition test, screening determines that formula is: the Radix Astragali: Radix Angelicae Sinensis: the root of herbaceous peony: Poria cocos: radix salviae miltiorrhizae: rheum officinale: XIGANCAO: Radix Bupleuri: RADIX SANGUISORBA: the leaf of bamboo=(5-10): 5:5:10:20:(10-15): 10:10:10:10, it is 0.1-0.5% that above formula coordinates the addition in pig starter feed in full price.In this invention, be mainly by adding important component to reduce the generation of diarrhoea, but because the reason of grice diarrhoea is relevant in factors such as its digestive system development prematurity, immunocompromised or digestive enzyme activity reductions, add Chinese medicine in the feed of piglet, the digestive system burden of piglet may be increased the weight of, the problem of grice diarrhoea cannot be fundamentally solved.
Summary of the invention
The object of the invention is to solve the problem of above-mentioned existence, provide a kind of and can effectively lower additive for microbe feedstuff of grice diarrhoea and preparation method thereof.
A first aspect of the present invention provides a kind of preparation method who reduces the additive for microbe feedstuff of grice diarrhoea, and its step comprises:
Step 1, the bacterium culture medium that preparation pH value is 5.0-7.0;
Step 2, is inoculated into bacterial classification in the bacterium culture medium that step 1 obtains and cultivates, and reaches 2,000,000,000/ml to total viable count;
Step 3, obtains bacterium powder by composite bacteria liquid freeze drying, is mixed to get finished product with milk powder, and in finished product, total viable bacteria is more than or equal to 2 * 10
9cfu/ml.
In above-mentioned step 1 composition of its composition of bacterium culture medium be in glucose, lactose, tryptone, bean sprout juice, sodium chloride, magnesium sulfate, ferrous sulfate, potassium dihydrogen phosphate one or more mixture and the water-soluble solution of surplus make.
In above-mentioned step 1, the ratio of bacterium culture medium composition components is preferably: glucose 0.1-5.0wt%, lactose 0.1-5.0wt%, tryptone 0.1-2wt%, bean sprout juice 0.1-4.0wt%, sodium chloride 0.1-1.0wt%, magnesium sulfate 0.01-0.05wt%, ferrous sulfate 0.01-0.10wt% and potassium dihydrogen phosphate 0.1-1.0wt%, by the water-soluble solution of surplus, make; More preferably: glucose 0.5-2.5wt%, lactose 0.5-2wt%, tryptone 0.5-1wt%, bean sprout juice 0.5-2.5wt%, sodium chloride 0.1-0.3wt%, magnesium sulfate 0.01-0.03wt%, ferrous sulfate 0.01-0.05wt% and potassium dihydrogen phosphate 0.2-0.8wt%, by the water-soluble solution of surplus, make.
The pH value of bacterium culture medium 5.5-6.5 more preferably in above-mentioned step 1, wherein pH value most preferably is 6.
In above-mentioned step 2, also comprise bacterial classification is carried out to sterilizing, wherein the temperature of sterilizing is preferably 100-150 ℃, 115-130 ℃ more preferably, and the time of sterilizing is preferably 10-40min, more preferably 15-30min.
In above-mentioned method, bacterial classification is preferably Lactobacillus plantarum and bifidobacterium adolescentis.Wherein, Lactobacillus plantarum is that deposit number is that (Lactobacillus plantarum) its deposit number is preferably the bacterial classification of ACCC No.11118; Its deposit number of bifidobacterium adolescentis (Bifidobacterium adolescentis) is preferably the bacterial classification of ACCC No.00215.
Bacterial classification described in above-mentioned step 2 is preferably Lactobacillus plantarum and bifidobacterium adolescentis, its mixed weight ratio is preferably Lactobacillus plantarum: bifidobacterium adolescentis=(30-70wt%): (30-70wt%), Lactobacillus plantarum more preferably: bifidobacterium adolescentis=(40-60wt%): (40-60wt%).
Cultivation described in above-mentioned step 2, cultivation temperature is preferably 32-40 ℃, 35-39 ℃ more preferably, incubation time is preferably 12-48h, more preferably 16-24h.
The mixed weight ratio that in above-mentioned step 2, bacterial classification is inoculated in culture medium is preferably (1-15): 100, more preferably (2-10): 100.
The milk powder mixing in above-mentioned step 3 is preferably skimmed milk power.
In above-mentioned step 3, the mixed weight ratio of bacterium powder and milk powder is preferably (2-30): 1, more preferably (5-20): 1, be packaged to be microorganism formulation finished product.
A second aspect of the present invention provides a kind of additive for microbe feedstuff that can reduce grice diarrhoea that uses said method to prepare, and comprises Lactobacillus plantarum and bifidobacterium adolescentis.Its mixed weight ratio is preferably Lactobacillus plantarum: bifidobacterium adolescentis=(30-70): (30-70), Lactobacillus plantarum more preferably: bifidobacterium adolescentis=(40-60): (40-60).
Above-mentioned piglet additive for microbe feedstuff can be in the rear directly gavage of piglet birth, and directly definite value intestine of young pigs, increases intestinal mucosa protective barrier function, reduces the infringement of external nuisance, significantly reduces grice diarrhoea; Can also make an addition to after mixing in the feed of piglet and carry out again feeding.
A third aspect of the present invention provides a kind of pig starter feed, comprises any one feed addictive described in second aspect of the present invention.
In a kind of preferred embodiment of third aspect of the present invention, in described pig starter feed, described feed addictive part by weight is 0.01-10%.
Of the present invention enteric modulators and probiotics viable bacteria preparation are integrated, containing any antibiotic, without the generation of worrying drug resistance.Use the intestinal microflora of additive for microbe feedstuff of the present invention in can control agent, reduce the pH value of enteron aisle, the breeding of increase beneficial bacterium, reduce the field planting of harmful bacteria, thereby effectively prevent and treat grice diarrhoea, improve the conversion ratio of pig starter feed, promote the growth of piglet.
The preparation method of feed addictive of the present invention, is by two probiotics Lactobacillus plantarums and bifidobacterium adolescentis composite fermentation, carries out freezing driving away after dry and obtains bacterium powder, and bacterium powder mixes in proportion with milk powder, obtains finished product.Method of the present invention is simple to operation, and the cycle of preparation is shorter, production cost is low, is applicable to large-scale manufacture level application.
Additive of the present invention can effectively strengthen pigling immunity, can improve the GI micro-ecological environment of piglet, improve animal to the digestion of breast milk nutriment and utilization ratio, later stage weanling pig growth and feed conversion are all had to good facilitation.
The preparation method of feed addictive of the present invention and additive also has following beneficial effect or advantage:
1, can effectively reduce the enteron aisle breathing problem of piglet, reduce grice diarrhoea rate;
2, strengthen piglet cellular immunity function, the differentiation of Promote immunity tissue, strengthens the resistance of piglet;
3, improve lactose utilization rate in breast milk, increase enteral nutrition and absorb;
4, improve the survival rate of weanling pig and reduce the ratio of cad pig;
5, promote intestinal villus growth, set up and stablize intestinal tract.
6, simple, the raw material of preparation is easy to get, and is suitable for large-scale production.
7, easy to use, can directly pour in piglet stomach or make an addition in the feed of piglet and carry out feeding.
Specific embodiment
Newborn piglet, because physique is poor, a little less than premunition, when being subject to cause pathogeny imcrobe infection, easily causes the generation of grice diarrhoea.Though antibiotic has advantages of rapid healing on grice diarrhoea, long-term a large amount of uses can cause antibiotic residue to pollute, and antibody-resistant bacterium produces, thereby breaks the colony balance in host, causes piglet autogenous infection.
In addition, pig farm health and epidemic prevention and feeding and management are unsound, the invasion and attack of pathogenic microorganism and propagation in a large number, also can be to piglet, and especially the grice diarrhoea of lactation causes very large impact.The piglet Huang, the dysentery characterized by white mucous stool that by enteropathogenic E．Coli, are caused are a kind of acute enteric infectious diseases, yellow dysentery is more common in the piglet of 3 age in days left and right, after piglet is ill, show as spirit depressed, arrange yellow loose stool, while grasping belly from the anus loose stool of emerging, if do not processed timely, piglet just there will be dehydration, becomes thin, dies of exhaustion and die, the situation of dying of illness.
Lactobacillus preparation is as one of common in recent years green additive, have and directly fill into exogenous beneficial bacterium, regulate animal self intestinal microecology balance, suppress harmful bacteria, thereby strengthening immunity of organisms and can promote the effects such as growing up healthy and sound of piglet, is the desirable approach of dealing with problems.
In additive for microbe feedstuff of the present invention, two strain bacterial strain Lactobacillus plantarums and bifidobacterium adolescentis can effectively suppress the infringement of the bacterial classifications such as Escherichia coli and Salmonella, in enteron aisle, set up biological barrier one, reduce poisonous and harmful substance and enter in body, thereby can better maintain intestinal eubiosis.Impel body to produce antibacterial substance, strengthen immunologic function, improve the resistance against diseases of piglet, significantly improve diarrhoea; Beneficial bacterium promotes intestine of young pigs to grow, and strengthens the activity of digestive ferment, improves the utilization rate to breast milk, improves daily gain.Beneficial microbe can also be a large amount of in enteron aisle the multiple nutrients materials such as synthetic vitamin, amino acid, use microbe additive of the present invention can obviously improve the outward appearance of piglet.
Below in conjunction with specific embodiment, the present invention will be further described, but not as limiting to the invention.
embodiment mono-
The selected bacterial classification of the embodiment of the present invention comprises:
Lactobacillus plantarum is that deposit number is that (Lactobacillus plantarum) its deposit number is the bacterial classification of ACCC No.11118;
Its deposit number of bifidobacterium adolescentis (Bifidobacterium adolescentis) is the bacterial classification of ACCC No.00215.
The preparation method that reduces the additive for microbe feedstuff of grice diarrhoea comprises the following steps:
The first step, by glucose 0.5wt%, lactose 1wt%, tryptone 0.6wt%, bean sprout juice 0.8wt%, sodium chloride 0.1wt%, magnesium sulfate 0.01wt%, ferrous sulfate 0.02 and potassium dihydrogen phosphate 0.6wt%, by the water-soluble solution of surplus, to make pH be 6.0 bacterium culture medium;
Second step: bacterium culture medium, 115 ℃ of sterilizings 30 minutes, is inoculated into bacterial classification in the bacterium culture medium that the first step obtains according to the part by weight of 5:100, and described bacterial classification is comprised of Lactobacillus plantarum 50 weight portions and bifidobacterium adolescentis 50 weight portions; At 36 ℃, cultivate 18 hours, when total viable count reaches 2,000,000,000/ml concentration, obtain composite bacteria liquid;
The 3rd step: composite bacteria liquid is obtained to bacterium powder through freeze drying and mix with skimmed milk power, the mixed weight ratio of bacterium powder and skimmed milk power is 9:1, is packaged to be microorganism formulation finished product, in finished product, total viable bacteria is more than or equal to 2 * 10
9cfu/ml.
embodiment bis-
The selected bacterial classification of the embodiment of the present invention comprises:
Lactobacillus plantarum is that deposit number is that (Lactobacillus plantarum) its deposit number is the bacterial classification of ACCC No.11118;
Its deposit number of bifidobacterium adolescentis (Bifidobacterium adolescentis) is the bacterial classification of ACCC No.00215.
The preparation method that reduces the additive for microbe feedstuff of grice diarrhoea comprises the following steps:
The first step, by glucose 1.0wt%, lactose 1.5wt%, tryptone 0.8wt%, bean sprout juice 0.8wt%, sodium chloride 0.1wt%, magnesium sulfate 0.01wt%, ferrous sulfate 0.02 and potassium dihydrogen phosphate 0.8wt%, by the water-soluble solution of surplus, to make pH be 6.0 bacterium culture medium;
Second step: bacterium culture medium, 121 ℃ of sterilizings 15 minutes, is inoculated into bacterial classification in the bacterium culture medium that the first step obtains according to the part by weight of 8:100, and described bacterial classification is comprised of Lactobacillus plantarum 45 weight portions and bifidobacterium adolescentis 55 weight portions; At 37 ℃, cultivate 16 hours, when total viable count reaches 2,000,000,000/ml concentration, obtain composite bacteria liquid;
The 3rd step: composite bacteria liquid is obtained to bacterium powder through freeze drying and mix with skimmed milk power, the mixed weight ratio of bacterium powder and skimmed milk power is 10:1, is packaged to be microorganism formulation finished product, in finished product, total viable bacteria is more than or equal to 2 * 10
9cfu/ml.
Experiment detects:
Experimental technique 1: select between the piglet (body weight is at 1.30-1.32kg) of 302 healthy firm births, wherein select 101 as a control group, control group newborn piglet is heavily on average 1.32kg; The reduction grice diarrhoea additive for microbe feedstuff that the embodiment of the present invention 1 and embodiment 2 are made with physiological saline according to standby after ten times of 1:9 dilutions; Choose 99 piglets, the average weight of embodiment 1 piglet is 1.31kg, the solution 0.5ml after the additive dilution that every newborn piglet makes with one-shot injector gavage embodiment 1; Choose remaining 102 piglets as embodiment 2 experimental group, embodiment 2 experimental group piglet average weights are 1.30kg, the solution 0.5ml after every additive made from one-shot injector gavage embodiment 2 of birth piglet dilutes.Detect after 25 days, the weight of three experimental group piglets, generation diarrhoea or dead quantity, calculate the diarrhea rate of the per day adding weight of piglet 25, every experimental group piglet and the death rate of every experimental group piglet.
Experimental result: as shown in table 1.
Table 1, the affect contrast experiment of the embodiment of the present invention on grice diarrhoea, weightening finish, the death rate
Interpretation: as can be seen from Table 1, embodiment 1 and embodiment 2 control group diarrhea rates have reduced respectively 47.4% and 58.7%.Embodiment 1 and embodiment 2 piglets occur without death.Embodiment 1 and embodiment 2 have improved respectively 20.65% and 22.82% than control group daily gain.
Experimental technique 2: the piglet obtaining in experimental technique 1 carries out the test experience of Immune Organs Index, as the test experience of index and spleen index and thymus index.
Experimental result: as shown in table 2.
Table 2, the affect contrast experiment of the embodiment of the present invention on piglet immunological organ index
| Project | Control group | Embodiment 1 | Embodiment 2 |
| Index and spleen index/% | 0.1544±0.0042 a | 0.1885±0.027 a | 0.1983±0.016 a |
| Thymus index/% | 0.1930±0.0045 a | 0.2134±0.0023 a | 0.2214±0.0018 a |
Interpretation: from table 2, in embodiment 1 and embodiment 2 piglet Thymus and spleen index all the utmost point significantly higher than control group (P<0.01); Between embodiment 1 and embodiment 2, there were significant differences for index and spleen index, and thymus index difference is not remarkable.
Above specific embodiments of the invention be have been described in detail, but it is as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that this practicality is carried out and alternative also all among category of the present invention.Therefore, equalization conversion and the modification done without departing from the spirit and scope of the invention, all should contain within the scope of the invention.
Claims (10)
1. a preparation method who reduces the additive for microbe feedstuff of grice diarrhoea, is characterized in that, the step of described method comprises:
Step 1, the bacterium culture medium that preparation pH value is 5.0-7.0;
Step 2, is inoculated into bacterial classification in the bacterium culture medium that step 1 obtains and cultivates, and reaches 2,000,000,000/ml to total viable count;
Step 3, obtains bacterium powder by the bacterium liquid freeze drying obtaining in step 2, is mixed to get finished product with milk powder, and in finished product, total viable bacteria is more than or equal to 2 * 10
9cfu/ml.
2. preparation method according to claim 1, it is characterized in that, in described step 1, the ratio of bacterium culture medium composition components is: glucose 0.5-2.5wt%, lactose 0.5-2wt%, tryptone 0.5-1wt%, bean sprout juice 0.5-2.5wt%, sodium chloride 0.1-0.3wt%, magnesium sulfate 0.01-0.03wt%, ferrous sulfate 0.01-0.05wt% and potassium dihydrogen phosphate 0.2-0.8wt%, by the water-soluble solution of surplus, make.
3. preparation method according to claim 1, is characterized in that, bacterial classification described in described step 2 comprises Lactobacillus plantarum and bifidobacterium adolescentis, and bacterial classification mixed weight ratio is Lactobacillus plantarum: bifidobacterium adolescentis=(30-70): (30-70).
4. preparation method according to claim 1, is characterized in that, in described step 2, the mixed weight ratio of bacterial classification and culture medium is (1-15): 100.
5. preparation method according to claim 1, is characterized in that, in described step 2, also comprises bacterial classification is carried out to sterilizing, and wherein the temperature of sterilizing is 100-150 ℃.
6. preparation method according to claim 5, is characterized in that, in described step 2, the time of sterilizing is 10-40min.
7. preparation method according to claim 1, is characterized in that, the cultivation temperature described in described step 2 is 32-40 ℃.
8. preparation method according to claim 7, is characterized in that, the incubation time described in described step 2 is 12-48h.
9. preparation method according to claim 1, is characterized in that, in described step 3, milk powder is skimmed milk power, and wherein the mixed weight ratio of bacterium powder and milk powder is (2-30): 1.
10. adopt the additive for microbe feedstuff that can reduce grice diarrhoea that in claim 1-9, the method described in any one prepares, it is characterized in that, described additive comprises Lactobacillus plantarum and bifidobacterium adolescentis.
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Application publication date: 20140212 |