CN108338277A - A kind of pet stomach is good for the preparation method of additive for microbe feedstuff - Google Patents
A kind of pet stomach is good for the preparation method of additive for microbe feedstuff Download PDFInfo
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- CN108338277A CN108338277A CN201810098899.3A CN201810098899A CN108338277A CN 108338277 A CN108338277 A CN 108338277A CN 201810098899 A CN201810098899 A CN 201810098899A CN 108338277 A CN108338277 A CN 108338277A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/40—Feeding-stuffs specially adapted for particular animals for carnivorous animals, e.g. cats or dogs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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Abstract
The invention discloses the preparation methods that a kind of pet stomach is good for additive for microbe feedstuff, include the following steps：Step 1：The composite bacteria being made of lactobacillus plantarum, bifidobacterium breve and saccharomyces cerevisiae is cultivated in composite bacteria culture medium, obtains composite bacteria liquid；Step 2：It is freeze-dried the composite bacteria liquid to obtain composite bacterium powder；Step 3：The composite bacterium powder is mixed in proportion with zytase, neutral proteinase and gamma amino butyric acid, obtains the additive for microbe feedstuff.The present invention directly definite value pet enteron aisle, increase intestinal mucosa protective barrier function can reduce the infringement of external nuisance, significantly reduce pet diarrhea phenomenon, enhance immunity of pets, reduce the generation of death of pet rate；The micro-ecological environment that pet gastrointestinal tract can also be improved reduces excrement stench, halitosis and the stress reaction of pet (wean, living environment change, vaccine inoculation, operation etc.).
Description
Technical field
The present invention relates to animal feed preparations and processing technique field more particularly to a kind of pet stomach to be good for microbiological feed
The preparation method of additive.
Background technology
In recent years, the pet quantity in China is increasingly huge, and dog food, cat food that domestic pets are eaten for a long time etc. are processing
Food contains food additives in processed food more, takes in various food additives for a long time so as to cause pet, and then cause and dote on
The enterogastric diseases of object；Simultaneously because antibiotic is widely used, body drug resistance and medicament residue make the normal bacterium of pet enteron aisle
Group's imbalance makes pet easy to produce constipation, have loose bowels, the intestines problems such as indigestion, enterogastritis, danger is brought to the health of pet
Evil.
Probiotics preparation is a kind of natural living microbe formulation, have it is nontoxic, without drug resistance, noresidue, without pair make
The characteristics of using.Probiotics can in the form of viable bacteria in animal alimentary canal by Reverse transcriptase pathogen, to enhance animal
The immunity of body, and the balance of animal intestinal micro-ecology is directly participated in, to safeguard enteron aisle normal function.
Invention content
In view of the deficiencies of the prior art, the present invention provides a kind of pet stomach to be good for the preparation side of additive for microbe feedstuff
Method directly definite value pet enteron aisle, increase intestinal mucosa protective barrier function can reduce the infringement of external nuisance, significantly reduce
Pet diarrhea phenomenon enhances immunity of pets, reduces the generation of death of pet rate；The Tiny ecosystem of pet gastrointestinal tract can also be improved
Environment reduces excrement stench, halitosis and the stress reaction of pet (wean, living environment change, vaccine inoculation, operation etc.).
To achieve the above object, the present invention uses following technical scheme：
A kind of pet stomach is good for the preparation method of additive for microbe feedstuff, includes the following steps：
Step 1：By the composite bacteria being made of lactobacillus plantarum, bifidobacterium breve and saccharomyces cerevisiae in composite bacteria culture
It is cultivated in base, obtains composite bacteria liquid；
Step 2：It is freeze-dried the composite bacteria liquid to obtain composite bacterium powder；
Step 3：The composite bacterium powder is mixed in proportion with zytase, neutral proteinase and gamma amino butyric acid, is obtained
To the additive for microbe feedstuff.
In order to advanced optimize above-mentioned technical proposal, the technical measures that the present invention is taken further include：
Further, the composite bacteria is bought in agriculture Microbiological Culture Collection administrative center, the plant breast bar
Bacterium (Lactobacillus plantarum) is the strain, the bifidobacterium breve that deposit number is ACCC 11118
(Bifidobacterium breve) is the strain that deposit number is ACCC 15700, the saccharomyces cerevisiae
(Saccharomyces cerevisiae) is the strain that deposit number is ATCC 26785.
Further, the group of each strain becomes in the composite bacteria：
40~60 parts by weight of lactobacillus plantarum
40~60 parts by weight of bifidobacterium breve
10~20 parts by weight of saccharomyces cerevisiae.
Further, the component of the composite bacteria culture medium and its weight proportion are：
Further, the pH value of the composite bacteria culture medium is 5.8-6.0.
Further, the culture of composite bacteria is by composite bacteria according to 2~10 in above-mentioned steps 1：100 weight ratio
It is inoculated into composite bacteria culture medium, cultivates 16-24 hours at 25-35 DEG C, when total viable count reaches 2,000,000,000/ml concentration, obtain
Obtain composite bacteria liquid；Further, the composite bacteria culture medium sterilizes 15-30 minutes before at 115-130 DEG C.
Further, composite bacterium powder described in above-mentioned steps 3 and zytase, neutral proteinase and gamma amino butyric acid
Weight ratio is：
Further, total viable bacteria >=1.0 × 10 in the additive for microbe feedstuff are obtained in above-mentioned steps 410cfu/ml。
On the other hand, the present invention also provides a kind of pet stomach obtained by above-mentioned preparation method to be good for microbiological feed addition
Agent.
Pet constitution is poor, premunition is weak, when by cause pathogeny imcrobe infection, most easily leads to the generation of pet diarrhea, resists
Though raw element has the advantages that rapid healing in pet diarrhea, long-term a large amount of uses can cause antibiotic residue to pollute, drug resistance
Bacterial strain generates, and to break the colony balance in host, causes pet autogenous infection.Additive for microbe feedstuff is as close
Common one of green additive over year, have directly fill into exogenous beneficial bacterium, adjust animal itself intestinal microecology balance,
Inhibit harmful bacteria, enhancing immunity of organisms, promote pet healthy growth effect, be the desirable approach solved the problems, such as.
Zytase and neutral proteinase used in the present invention can promote the digestion and absorption of the fed food of pet,
And maintain pet internal organ institute functional, including：Cell repair, anti-inflammatory toxin expelling, metabolism, improve immunity, generate energy,
It promotes blood circulation；The activity of phosphatidase can be improved in used gamma amino butyric acid, to promote the energy generation of animal brain
It thanks, activates brain blood flow, increase oxygen supply amount, finally restore function of brain cell, improve nervous function, can inhibit brain stem respiratory center
Integration, so that respiratory rate is slowed down, mitigate hot in heat stress of animal and pant, increase weight, improve survival rate,
And gamma amino butyric acid also has phagostimulating effect.
Bacterial strain used in the additive for microbe feedstuff of the present invention can effectively inhibit the bacterium such as Escherichia coli and Salmonella
Infringement, set up one of biological barrier in enteron aisle, reduce poisonous and harmful substance and enter in pet body, maintain enteron aisle ecology flat
Weighing apparatus, and body can be promoted to generate antibacterial substance, enhance immune function, improves the disease resistance of pet, significantly improve abdomen
It rushes down；Beneficial bacterium promotes pet intestinal growth, enhances the activity of digestive ferment, improves the nutrient absorption of animal, reduces stool odor.And
And beneficial microbe can synthesize a variety of nutriments such as vitamin, amino acid in enteron aisle, can be obviously improved the skin of pet
Hair.
Technical solutions according to the invention have advantageous effect below：
1) pet stomach of the invention is good for additive for microbe feedstuff, can compete the attached of gut epithelium with harmful microorganism
Position, beneficial bacterium dominant microflora is established, inhibits pathogenic bacteria growth, avoids enteron aisle stiff reaction, reduce the wind of bacillary enteritis
Danger.
2) pet stomach of the invention is good for additive for microbe feedstuff, can decompose, convert, being harmful to using what metabolism generated
Substance reduces accumulation of the noxious material in pet body, protects liver；Ammonia, nitrogen concentration in pet enteron aisle are reduced, to the row of reduction
Let out the stink of object.
3) pet stomach of the invention is good for additive for microbe feedstuff, can irritate intestinal mucosa cell and generate panimmunity
The factor, to improve resistance of the pet to disease.
4) pet stomach of the invention is good for additive for microbe feedstuff, and the organic acid of generation can build enteron aisle acid or alkali environment,
Be conducive to the absorption of the nutrition such as minerals, while B family vitamin can also be generated, to supplement the nutrients needed for pet supplement.
5) pet stomach of the invention is good for additive for microbe feedstuff, can increase pet and become to conditions such as food, environment
The adaptability of change reduces or mitigates stiff reaction, and the comprehensive health for improving body is horizontal.
Specific implementation mode
The present invention provides the preparation methods that a kind of pet stomach is good for additive for microbe feedstuff, below by specific implementation
The present invention is described in more detail in example, and for a better understanding of the present invention, but following embodiments are not intended to limit this
Invention scope.
Embodiment 1
Step 1：By molasses 0.5wt%, lactose 1wt%, tryptone 0.6wt%, corn steep liquor 0.8wt%, sodium chloride
0.1wt%, magnesium sulfate 0.01wt%, ferrous sulfate 0.02wt% and potassium dihydrogen phosphate 0.6wt%, are made of the water dissolution of surplus
The composite bacteria culture medium that pH is 6.0, composite bacteria culture medium is sterilized 30 minutes at 115 DEG C, by composite bacteria according to 5：100
Weight ratio be inoculated into composite bacteria culture medium, the composite bacteria is by 50 parts by weight of lactobacillus plantarum, bifidobacterium breve
40 parts by weight and 10 parts by weight of saccharomyces cerevisiae composition；It is cultivated 18 hours at 36 DEG C, when total viable count reaches 2,000,000,000/ml concentration
Obtain composite bacteria liquid；
Step 2：The composite bacteria liquid that step 1 obtains is obtained into composite bacterium powder by freeze-drying；
Step 3：According to obtained composite bacterium powder and skimmed milk power 9：1 weight ratio mixing, is packaged to be microorganism system
Agent finished product, total viable bacteria is more than or equal to 1.0 × 10 in finished product10cfu/ml。
Embodiment 2
Step 1：By molasses 1.0wt%, lactose 1.5wt%, tryptone 0.8wt%, corn steep liquor 0.8wt%, sodium chloride
0.1wt%, magnesium sulfate 0.01wt%, ferrous sulfate 0.02wt% and potassium dihydrogen phosphate 0.8wt%, are made of the water dissolution of surplus
The composite bacteria culture medium that pH is 6.0, composite bacteria culture medium is sterilized 15 minutes at 121 DEG C, by composite bacteria according to 8：100
Weight ratio be inoculated into composite bacteria culture medium, the composite bacteria is by 45 parts by weight of lactobacillus plantarum, bifidobacterium breve
40 parts by weight and 15 parts by weight of saccharomyces cerevisiae composition；It is cultivated 16 hours at 30 DEG C, when total viable count reaches 2,000,000,000/ml concentration
Obtain composite bacteria liquid；
Step 2：The composite bacteria liquid that step 1 obtains is obtained into composite bacterium powder by freeze-drying；
Step 3：According to obtain 50 parts by weight of composite bacterium powder, 20 parts by weight of zytase, 20 parts by weight of neutral proteinase and
10 parts by weight of gamma amino butyric acid mix, and are packaged to be microorganism formulation finished product, in finished product total viable bacteria be more than or equal to 1.0 ×
1010cfu/ml。
Embodiment 3
The present embodiment be verification embodiment, subjects be cat and dog,
Control group is fed using cat food or dog food；
For experimental group while being fed using cat food or dog food, cat experimental group feeds the micro- of the preparation of embodiment 2 daily
0.5 gram of biological feed additive, dog experimental group feed 1.0 grams of the additive for microbe feedstuff of the preparation of embodiment 2 daily.
Specific experiment the results are shown in Table one and table two.
The influence of table a pair of pet daily gain, diarrhea rate, the death rate
Cat experimental group reduces 53.47% respectively than control group column home ammonia, diarrhea rate it can be seen from the data of table one
With 76.8%；Dog experimental group reduces 63.89% and 71.6%, cat experimental group ratio respectively than control group column home ammonia, diarrhea rate
Control group daily gain improves 6.12%, and dog experimental group improves 10.28% than control group daily gain.And compared to control group, institute
There is experimental group all without animal dead.
Influence of the table two to pet Immune Organs Index
It can be seen from the data of table two cat of experimental group, dog pet Thymus and spleen index pole be significantly higher than pair
According to group (P<0.01).
It is able to know that by above example, a kind of pet stomach of the invention is good for the preparation side of additive for microbe feedstuff
Method directly definite value pet enteron aisle, increase intestinal mucosa protective barrier function can reduce the infringement of external nuisance, significantly reduce
Pet diarrhea phenomenon enhances immunity of pets, reduces the generation of death of pet rate.
Specific embodiments of the present invention are described in detail above, but it is intended only as example, the present invention is simultaneously unlimited
It is formed on particular embodiments described above.To those skilled in the art, it is any to the equivalent modifications that carry out of the present invention and
It substitutes also all among scope of the invention.Therefore, without departing from the spirit and scope of the invention made by impartial conversion and
Modification, all should be contained within the scope of the invention.
Claims (10)
1. a kind of pet stomach is good for the preparation method of additive for microbe feedstuff, which is characterized in that include the following steps：
Step 1：By the composite bacteria being made of lactobacillus plantarum, bifidobacterium breve and saccharomyces cerevisiae in composite bacteria culture medium
It is cultivated, obtains composite bacteria liquid；
Step 2：It is freeze-dried the composite bacteria liquid to obtain composite bacterium powder；
Step 3：The composite bacterium powder is mixed in proportion with zytase, neutral proteinase and gamma amino butyric acid, obtains institute
State additive for microbe feedstuff.
2. preparation method according to claim 1, which is characterized in that the composite bacteria is bought in agriculture microbial bacteria
Kind preservation administrative center, it is ACCC 11118 that the lactobacillus plantarum (Lactobacillus plantarum), which is deposit number,
Strain, the bifidobacterium breve (Bifidobacterium breve) be strain that deposit number is ACCC 15700, it is described
Saccharomyces cerevisiae (Saccharomyces cerevisiae) is the strain that deposit number is ATCC 26785.
3. preparation method described in claim 1, which is characterized in that the group of each strain becomes in the composite bacteria：
40~60 parts by weight of lactobacillus plantarum
40~60 parts by weight of bifidobacterium breve
10~20 parts by weight of saccharomyces cerevisiae.
4. preparation method described in claim 1, which is characterized in that the component and its weight proportion of the composite bacteria culture medium
For：
Surplus is water.
5. the preparation method described in claim 4, which is characterized in that the pH value of the composite bacteria culture medium is 5.8-6.0.
6. preparation method described in claim 1, which is characterized in that the culture of composite bacteria is by compound bacteria in above-mentioned steps 1
Kind is according to 2~10：100 weight ratio is inoculated into composite bacteria culture medium, is cultivated 16-24 hours at 25-35 DEG C, is worked as viable bacteria
When sum reaches 2,000,000,000/ml concentration, composite bacteria liquid is obtained.
7. the preparation method described in claim 6, which is characterized in that the composite bacteria culture medium is using preceding at 115-130 DEG C
Sterilizing 15-30 minutes.
8. preparation method described in claim 1, which is characterized in that composite bacterium powder described in above-mentioned steps 3 and zytase, in
Property protease and the weight ratio of gamma amino butyric acid are：
9. preparation method described in claim 1, which is characterized in that obtain the additive for microbe feedstuff in above-mentioned steps 4
In total viable bacteria >=1.0 × 1010cfu/ml。
Add 10. the pet stomach that a kind of preparation method according to any one of claim 1-9 obtains is good for microbiological feed
Add agent.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810098899.3A CN108338277A (en) | 2018-01-31 | 2018-01-31 | A kind of pet stomach is good for the preparation method of additive for microbe feedstuff |
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| CN201810098899.3A CN108338277A (en) | 2018-01-31 | 2018-01-31 | A kind of pet stomach is good for the preparation method of additive for microbe feedstuff |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109548956A (en) * | 2018-12-29 | 2019-04-02 | 深圳合民生物科技有限公司 | A kind of pet nutrition liquid and preparation method thereof |
| CN114601032A (en) * | 2022-04-07 | 2022-06-10 | 深圳市豆柴宠物用品有限公司 | Additive with function of gastrointestinal anti-stress |
| CN114869926A (en) * | 2021-06-26 | 2022-08-09 | 临沂金翠兽药有限公司 | Plant composition for nourishing intestines and stomach of animals as well as preparation method and using method thereof |
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| CN101219154A (en) * | 2007-01-09 | 2008-07-16 | 上海创博生态工程有限公司 | Microorganism additive agent for improving phylactic power of pets and accelerating growth |
| CN101473906A (en) * | 2009-01-20 | 2009-07-08 | 天津市伯曼宠物饲料有限公司 | Nutritive composition for reducing production of bad odour from pet |
| CN107137530A (en) * | 2017-07-12 | 2017-09-08 | 天津市聚星康华医药科技有限公司 | One kind is used for pet composition antianxity |
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2018
- 2018-01-31 CN CN201810098899.3A patent/CN108338277A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101219154A (en) * | 2007-01-09 | 2008-07-16 | 上海创博生态工程有限公司 | Microorganism additive agent for improving phylactic power of pets and accelerating growth |
| CN101473906A (en) * | 2009-01-20 | 2009-07-08 | 天津市伯曼宠物饲料有限公司 | Nutritive composition for reducing production of bad odour from pet |
| CN107137530A (en) * | 2017-07-12 | 2017-09-08 | 天津市聚星康华医药科技有限公司 | One kind is used for pet composition antianxity |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109548956A (en) * | 2018-12-29 | 2019-04-02 | 深圳合民生物科技有限公司 | A kind of pet nutrition liquid and preparation method thereof |
| CN114869926A (en) * | 2021-06-26 | 2022-08-09 | 临沂金翠兽药有限公司 | Plant composition for nourishing intestines and stomach of animals as well as preparation method and using method thereof |
| CN114601032A (en) * | 2022-04-07 | 2022-06-10 | 深圳市豆柴宠物用品有限公司 | Additive with function of gastrointestinal anti-stress |
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