CN111802514A - Microbial modifier and preparation method and application thereof - Google Patents
Microbial modifier and preparation method and application thereof Download PDFInfo
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- CN111802514A CN111802514A CN202010644622.3A CN202010644622A CN111802514A CN 111802514 A CN111802514 A CN 111802514A CN 202010644622 A CN202010644622 A CN 202010644622A CN 111802514 A CN111802514 A CN 111802514A
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses a microbial modifier and a preparation method and application thereof. The microbial improver comprises: bacillus subtilis, saccharomyces cerevisiae, clostridium butyricum, lactobacillus plantarum, pediococcus pentosaceus, enterococcus faecalis, bacillus aceticus, bifidobacterium, lactobacillus acidophilus, lactobacillus casei, photosynthetic bacteria, actinomycetes, clostridium butyricum and bacillus coagulans. The microbial modifier provided by the invention has good effect due to the mutual matching and interaction of various floras, and can be widely applied to the feeding of various poultry or livestock. Meanwhile, the sanitary area modifier can obviously enhance the immunity of poultry or livestock, promote nutrient absorption, improve the utilization rate of feed and improve the meat quality; and has good therapeutic effect on the existing common diseases, even seriously ill poultry or livestock, and can obviously improve the economic benefit of farmers.
Description
Technical Field
The invention belongs to the technical field of microorganisms. More particularly, relates to a microbial modifier, a preparation method and application thereof.
Background
In livestock and poultry breeding industry, viral and bacterial diseases are common frequently encountered diseases, and in order to improve the health-care immunity of livestock and poultry, a measure is usually adopted that some conventional feed additives containing antibiotics, growth-promoting hormones, chemical drugs and the like are added into livestock and poultry feed. With the improvement of the living standard of human beings, the food safety problem is concerned. The use of additives such as antibiotics, growth-promoting hormones, chemical drugs and the like in the feed not only affects the healthy growth of animals and the quality of meat products, but also causes certain potential safety hazards to food safety, and greatly threatens the health of human beings.
The microbial additive is a preparation without toxicity, side effect, drug resistance and environmental pollution, and has the functions of maintaining intestinal microecological balance, promoting animal growth, producing digestive enzyme and vitamins, raising feed conversion rate and raising animal efficiency. And part of functional bacteria have the functions of inhibiting harmful bacteria and enhancing the immune function of organisms. Plays an increasingly important role in replacing antibiotics and reducing the dosage of chemical synthetic drugs in livestock breeding.
However, the microbial additives in the current market only have the functions of improving the intestinal digestion function of poultry, enhancing immunity and preventing and protecting health, but do not have the treatment effect on sick poultry, for example, patent 201610110688.8 discloses a compound microbial inoculum for enhancing animal immunity, which can adjust the flora balance of animal intestinal tracts, enhance immunity and stress capacity, adjust the functions of the digestive system of animals and improve the utilization rate of feed, but does not have the treatment effect on sick poultry. Meanwhile, the dilution concentration of the current product cannot exceed 100 times, otherwise the beneficial effect is greatly reduced, thereby causing higher logistics transportation cost.
Disclosure of Invention
Aiming at the defects and shortcomings of the prior art, the microbial modifier and the preparation method thereof are provided. The microbial modifier provided by the invention not only obviously improves the intestinal digestion function of livestock and poultry and enhances the immunity of the livestock and poultry, but also has a good treatment effect on existing common diseases, even seriously ill poultry or livestock, and simultaneously improves the meat quality of the livestock and poultry and greatly improves the economic benefit of farmers. Meanwhile, the concentration of the microbial modifier is as high as 500-800 times, the logistics transportation cost is obviously reduced, and the production cost is saved.
The invention aims to provide a microbial improver.
The invention also aims to provide a preparation method of the microbial improver.
The invention also aims to provide the application of the microbial modifier in poultry feeding and livestock feeding.
The above purpose of the invention is realized by the following technical scheme:
a microbial improver, comprising: bacillus subtilis (Bacillus subtilis), Saccharomyces cerevisiae (Saccharomyces cerevisiae), Clostridium butyricum (Clostridium butyricum), Lactobacillus plantarum (Lactobacillus plantarum), Pediococcus pentosaceus (Pediococcus pentosaceus), Enterococcus faecalis (Enterococcus faecalis), Acetobacter aceti (Acetobacter basicum), Bifidobacterium (Bifidobacterium), Lactobacillus (Lactobacillus Beijerinck), Lactobacillus acidophilus (Lactobacillus acidophilus), Lactobacillus casei (Lactobacillus casei), Photosynthetic bacteria (Photosyntactical bacteria), Actinomycetes (Actinomycete), Clostridium butyricum (Clostridium butyricum), and Bacillus coagulans (Bacillus coagulans).
Preferably, the present inventionThe total number of viable colonies of the microbial improver is not less than 1x109CFU/mL, the invention found that when the total number of viable colonies was greater than 1X109When CFU/mL is adopted, the microbial modifier can be used for realizing higher promotion of the intestinal digestion function of livestock and improving the immunity of the livestock, and has better treatment effect on sick poultry or livestock.
Preferably, in the microbial improver: the viable count of the bacillus subtilis is 1x109—8x1010CFU/mL, the number of live Saccharomyces cerevisiae is 3x104—8x106CFU/mL, Clostridium butyricum viable count of 2x106—9x108CFU/mL, viable count of Lactobacillus plantarum 1x104—5x106CFU/mL, viable count of Pediococcus pentosaceus 2X106—5x108CFU/mL, viable count of enterococcus faecalis 2x106—1x108CFU/mL, viable count of acetobacter 3x106—7x108CFU/mL, viable count of Bifidobacterium 1 × 106—5x109CFU/mL, viable count of lactobacillus 2x106—6x108CFU/mL, viable count of Lactobacillus acidophilus 3x108—8x1010CFU/mL, viable count of Lactobacillus casei 1x104—6x106CFU/mL, photosynthetic bacteria viable count of 1x104—9x106CFU/mL, viable count of actinomycete 2x105—9x107CFU/mL, the number of viable bacteria of Clostridium butyricum is 5x106—5x108CFU/mL, viable count of Bacillus coagulans of 1x106—8x109CFU/mL。
Further, preferably, in the microbial improver: the viable count of the bacillus subtilis is 5x109—2x1010CFU/mL, the number of live Saccharomyces cerevisiae 5x105—2x106CFU/mL, Clostridium butyricum viable count of 8x107—5x108CFU/mL, viable count of Lactobacillus plantarum 5x105—1x106CFU/mL, viable count of Pediococcus pentosaceus 3X106—9x107CFU/mL, viable count of enterococcus faecalis 8x106—1x107CFU/mL, viable count of Acetobacter 6x106—9x107CFU/mL, viable count of Bifidobacterium 5 × 107—1x109CFU/mL, viable count of lactobacillus 8x107—1x108CFU/mL, viable count of Lactobacillus acidophilus 5x109—1x1010CFU/mL, viable count of Lactobacillus casei 5x104—1x106CFU/mL, 6x10 photosynthetic bacteria viable count5—1x106CFU/mL, viable count of actinomycete 1x106—5x107CFU/mL, the number of viable bacteria of Clostridium butyricum is 1x107—1x108CFU/mL, viable count of Bacillus coagulans of 1x107—5x109CFU/mL。
Still further, preferably, in the microbial improver: the viable count of the bacillus subtilis is 8x109CFU/mL, the number of live bacteria of saccharomyces cerevisiae is 1x106CFU/mL, Clostridium butyricum viable count of 2x108CFU/mL, viable count of Lactobacillus plantarum 7x105CFU/mL, viable count of Pediococcus pentosaceus 2X107CFU/mL, viable count of enterococcus faecalis 9x106CFU/mL, viable count of Acetobacter 2x107CFU/mL, viable count of Bifidobacterium 4x108CFU/mL, viable count of lactobacillus 9x107CFU/mL, viable count of Lactobacillus acidophilus 7x109CFU/mL, viable count of Lactobacillus casei 5x105CFU/mL, photosynthetic bacteria viable count of 8x105CFU/mL, viable count of actinomycete 3x107CFU/mL, the number of viable bacteria of Clostridium butyricum is 8x107CFU/mL, the viable count of the bacillus coagulans is 6x108CFU/mL。
Further, as a preferable scheme, the invention provides a preparation method of the microbial modifier, which specifically comprises the following steps:
(1) solid slant culture: respectively inoculating bacillus subtilis, saccharomyces cerevisiae, clostridium butyricum, lactobacillus plantarum, pediococcus pentosaceus, enterococcus faecalis, bacillus aceticus, bifidobacterium, lactobacillus acidophilus, lactobacillus casei, photosynthetic bacteria, actinomycetes, clostridium butyricum and bacillus coagulans and culturing the inoculated bacillus subtilis, the saccharomyces cerevisiae, the clostridium butyricum, the lactobacillus plantarum, the pediococcus pentosaceus, the lactobacillus casei, the photosynthetic bacteria, the actinomycetes, the clostridium butyricum and the bacillus coagulans with a solid slant culture medium to fully activate strains;
(2) inoculating the activated bacillus subtilis, saccharomyces cerevisiae, clostridium butyricum, lactobacillus plantarum, pediococcus pentosaceus, enterococcus faecalis, bacillus aceticus, bifidobacterium, lactobacillus acidophilus, lactobacillus casei, photosynthetic bacteria, actinomycetes, clostridium butyricum and bacillus coagulans in the step (1) into a sterile liquid culture medium for mixed culture to obtain a total mixed flora;
(3) inoculating the total mixed flora in the step (2) to a fermentation culture medium by 5% of volume, carrying out fermentation culture for 15-18 days at the temperature of 25-30 ℃, continuously shaking or stirring in the fermentation culture process to obtain a bacterial liquid, centrifuging, concentrating, and washing the bacterial liquid with distilled water at normal temperature to obtain the microbial modifier.
Preferably, the solid slant culture medium comprises tryptone 15g, beef extract 8g, glucose 20g, sodium chloride 0.5g, distilled water to make up 1000mL, agar 10g, pH7.4, sterilization conditions: 30min at 121 ℃; the formula of the liquid culture medium comprises: 15g of peptone, 8g of beef extract, 20g of glucose, 0.5g of sodium chloride, 1000mL of distilled water, pH7.4, and sterilization conditions: 30min at 121 ℃; the fermentation medium comprises peptone 20g/L, beef extract 10g/L, yeast 1g/L, glucose 5g/L, fructo-oligosaccharide 5g/L, pH7.2, and sterilization conditions are as follows: 121 ℃ and 30 min.
Preferably, the total mixed flora comprises bacillus subtilis, saccharomyces cerevisiae, clostridium butyricum, lactobacillus plantarum, pediococcus pentosaceus, enterococcus faecalis, bacillus aceticus, bifidobacterium, lactobacillus acidophilus, lactobacillus casei, photosynthetic bacteria, actinomycetes, clostridium butyricum and bacillus coagulans in a volume ratio of: (20-40):(1-20):(1-20):(1-10):(1-15): (1-20): 1-15): 1-30): 1-20): 5-30): 1-20): 1-25): 1-15): 5-20). More preferably, the microbial inoculum is mixed in a volume ratio of 25:2:5: 5:5:6:11:3:10:5:5:2:4:10, and the mixed microbial inoculum is mixed in the ratio, so that the microbial inoculum has a better strain number composition, and thus, the best cultivation effect of poultry or livestock is achieved, such as improvement of immunity of poultry or livestock, promotion of gastrointestinal digestion, improvement of meat quality, treatment of diseased poultry or livestock, and the like.
The pH value of any microbial modifier is less than or equal to 3.8, so that the invasion of mixed bacteria is effectively avoided, and the stability of the product is enhanced. In addition, all indexes of the microbial modifier reach the specification of No. 75 of the State administration of quality supervision, inspection and quarantine [2005 ].
The practice of the invention shows that the microbial modifier can obviously regulate the gastrointestinal flora of poultry or livestock, realize gastrointestinal balance, promote the nutrient absorption of the poultry or the livestock, improve the daily gain, improve the feed utilization rate, simultaneously improve the body resistance capacity, effectively treat bacterial diseases, obviously improve the economic benefit and effectively reduce the morbidity and mortality of the poultry or the livestock. Therefore, the use of the microbial improver according to the invention in poultry or livestock breeding should also be within the scope of protection.
Preferably, the microbial modifier is applied to the feeding of poultry or livestock such as chicken, duck, goose, pigeon, partridge, quail, pig, cattle, sheep and the like.
As a preferable mode, the method for using the microbial improver in the poultry or livestock raising comprises the following steps: (1) direct drinking: adding a microbial modifier into water 1: diluting the mixture by 800 times at a ratio of 500-: 200-300 times of dilution ratio; (2) mixing materials: the microbial modifier is prepared by the following steps of 1: after being diluted by water in a proportion of 500 times of 300-.
The invention has the following beneficial effects:
the microbial modifier is obtained by continuously screening and optimizing microbial floras and the proportion thereof, and can be widely applied to feeding of various poultry or livestock.
The microbial modifier can effectively adjust the balance of intestinal flora of poultry or livestock, enhance the immunity of the poultry or the livestock, promote nutrient absorption, improve the utilization rate of feed and improve the meat quality; and has good therapeutic effect on the existing common diseases, even seriously ill poultry or livestock, and can obviously improve the economic benefit of farmers.
In addition, the concentration degree of the microbial modifier is as high as 500-800 times, which is obviously higher than that of the existing product, thereby greatly reducing the logistics transportation cost and saving the production cost.
Detailed Description
The present invention is further illustrated by the following specific examples, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
Unless otherwise indicated, reagents and materials used in the following examples are commercially available.
Example 1 preparation of microbial inoculum
(1) Solid slant culture: respectively inoculating bacillus subtilis, saccharomyces cerevisiae, clostridium butyricum, lactobacillus plantarum, pediococcus pentosaceus, enterococcus faecalis, bacillus aceticus, bifidobacterium, lactobacillus acidophilus, lactobacillus casei, photosynthetic bacteria, actinomycetes, clostridium butyricum and bacillus coagulans, and culturing the inoculated bacillus subtilis, the saccharomyces cerevisiae, the clostridium butyricum, the lactobacillus casei, the photosynthetic bacteria, the actinomycetes, the clostridium butyricum and the bacillus coagulans with a solid slant culture medium to fully activate strains, wherein the solid slant culture medium comprises tryptone 15g, beef extract 8g, glucose 20g, sodium chloride 0.5g, distilled water which is supplemented by 1000mL, agar 10g, pH7.4, and sterilization conditions are as follows: 30min at 121 ℃;
(2) inoculating the bacillus subtilis, saccharomyces cerevisiae, clostridium butyricum, lactobacillus plantarum, pediococcus pentosaceus, enterococcus faecalis, bacillus aceticus, bifidobacterium, lactobacillus acidophilus, lactobacillus casei, photosynthetic bacteria, actinomycetes, clostridium butyricum and bacillus coagulans activated in the step (1) into an aseptic liquid culture medium for mixed culture to obtain a total mixed flora, wherein the volume ratio of the bifidobacterium, the lactobacillus acidophilus, the clostridium butyricum, the bacillus coagulans and the lactobacillus in the total mixed flora is as follows: (20-40):(1-20):(1-20):(1-10):(1-15): (1-20): 1-15): 1-30): 1-20): 5-30): 1-20): 1-25): 1-15): 5-20); wherein the formula of the liquid culture medium comprises: 15g of peptone, 8g of beef extract, 20g of glucose, 0.5g of sodium chloride, 1000mL of distilled water, pH7.4, and sterilization conditions: 30min at 121 ℃;
(3) inoculating the total mixed flora in the step (2) to a fermentation culture medium by 5% of volume, performing fermentation culture for 15-18 days at the temperature of 25-30 ℃, continuously shaking or stirring in the fermentation culture process to obtain a bacterial liquid, centrifuging, concentrating, and washing the thalli with distilled water at normal temperature to obtain a microbial modifier, wherein the fermentation culture medium comprises peptone 20g/L, beef extract 10g/L, yeast 1g/L, glucose 5g/L, fructo-oligosaccharide 5g/L, pH7.2, and sterilization conditions are as follows: 121 ℃ and 30 min.
The pH value of the prepared microbial modifier is less than or equal to 3.8, and the total viable bacteria colony number is not less than 1x109CFU/mL, wherein the viable count of the bacillus subtilis is 1x109—8x1010CFU/mL, the number of live Saccharomyces cerevisiae is 3x104—8x106CFU/mL, Clostridium butyricum viable count of 2x106—9x108CFU/mL, viable count of Lactobacillus plantarum 1x104—5x106CFU/mL, viable count of Pediococcus pentosaceus 2X106—5x108CFU/mL, viable count of enterococcus faecalis 2x106—1x108CFU/mL, viable count of acetobacter 3x106—7x108CFU/mL, viable count of Bifidobacterium 1 × 106—5x109CFU/mL, viable count of lactobacillus 2x106—6x108CFU/mL, viable count of Lactobacillus acidophilus 3x108—8x1010CFU/mL, viable count of Lactobacillus casei 1x104—6x106CFU/mL, photosynthetic bacteria viable count of 1x104—9x106CFU/mL, viable count of actinomycete 2x105—9x107CFU/mL, the number of viable bacteria of Clostridium butyricum is 5x106—5x108CFU/mL, viable count of Bacillus coagulans of 1x106—8x109CFU/mL。
Example 2 microbial inoculum
The microbial modifier is in the form of a bacterial liquid and comprises: the viable count of the bacillus subtilis is 1x109—8x1010CFU/mL, the number of live Saccharomyces cerevisiae is 3x104—8x106CFU/mL, Clostridium butyricum viable count of 2x106—9x108CFU/mL, viable count of Lactobacillus plantarum 1x104—5x106CFU/mL, viable count of Pediococcus pentosaceus 2X106—5x108CFU/mL, viable count of enterococcus faecalis 2x106—1x108CFU/mL, viable count of Acetobacter3x106—7x108CFU/mL, viable count of Bifidobacterium 1 × 106—5x109CFU/mL, viable count of lactobacillus 2x106—6x108CFU/mL, viable count of Lactobacillus acidophilus 3x108—8x1010CFU/mL, viable count of Lactobacillus casei 1x104—6x106CFU/mL, photosynthetic bacteria viable count of 1x104—9x106CFU/mL, viable count of actinomycete 2x105—9x107CFU/mL, the number of viable bacteria of Clostridium butyricum is 5x106—5x108CFU/mL, viable count of Bacillus coagulans of 1x106—8x109CFU/mL。
Microorganism-modified liquid microbial inoculum 1: the viable count of the bacillus subtilis is 8x109CFU/mL, the number of live bacteria of saccharomyces cerevisiae is 1x106CFU/mL, Clostridium butyricum viable count of 2x108CFU/mL, viable count of Lactobacillus plantarum 7x105CFU/mL, viable count of Pediococcus pentosaceus 2X107CFU/mL, viable count of enterococcus faecalis 9x106CFU/mL, viable count of Acetobacter 2x107CFU/mL, viable count of Bifidobacterium 4x108CFU/mL, viable count of lactobacillus 9x107CFU/mL, viable count of Lactobacillus acidophilus 7x109CFU/mL, viable count of Lactobacillus casei 5x105CFU/mL, photosynthetic bacteria viable count of 8x105CFU/mL, viable count of actinomycete 3x107CFU/mL, the number of viable bacteria of Clostridium butyricum is 8x107CFU/mL, the viable count of the bacillus coagulans is 6x108CFU/mL。
And (2) microbial inoculum: the viable count of the bacillus subtilis is 2x1010CFU/mL, the number of live Saccharomyces cerevisiae 5x105CFU/mL, Clostridium butyricum viable count of 8x107CFU/mL, viable count of Lactobacillus plantarum 5x105CFU/mL, viable count of Pediococcus pentosaceus 9x107CFU/mL, viable count of enterococcus faecalis 8x106CFU/mL, viable count of Acetobacter 6x106CFU/mL, viable count of Bifidobacterium 5 × 107CFU/mL, viable count of lactobacillus 8x107CFU/mL, viable count of Lactobacillus acidophilus 5x109CFU/mL, viable count of Lactobacillus casei 1x106CFU/mL, photosynthetic bacteria viable count of 1x106CFU/mL, viable count of actinomycete 5x107CFU/mL, the number of viable bacteria of Clostridium butyricum is 1x108CFU/mL, the viable count of the bacillus coagulans is 5x109CFU/mL。
And (3) microbial inoculum: the viable count of the bacillus subtilis is 5x109CFU/mL, the number of live Saccharomyces cerevisiae bacteria is 2x106CFU/mL, the number of viable bacteria of clostridium butyricum is 5x108CFU/mL, viable count of Lactobacillus plantarum 1x106CFU/mL, viable count of Pediococcus pentosaceus 3X106CFU/mL, viable count of enterococcus faecalis 1x107CFU/mL, viable count of Acetobacter 9x107CFU/mL, viable count of Bifidobacterium 1 × 109CFU/mL, viable count of lactobacillus 1 × 108CFU/mL, viable count of Lactobacillus acidophilus 1x1010CFU/mL, viable count of Lactobacillus casei 5x104CFU/mL, 6x10 photosynthetic bacteria viable count5CFU/mL, viable count of actinomycete 1x106CFU/mL, the number of viable bacteria of Clostridium butyricum is 1x107CFU/mL, viable count of Bacillus coagulans of 1x107CFU/mL。
And (4) microbial inoculum: the viable count of the bacillus subtilis is 8x1010CFU/mL, the number of live Saccharomyces cerevisiae is 3x104CFU/mL, Clostridium butyricum viable count of 2x106CFU/mL, viable count of Lactobacillus plantarum 1x104CFU/mL, viable count of Pediococcus pentosaceus 5x108CFU/mL, viable count of enterococcus faecalis 2x106CFU/mL, viable count of acetobacter 3x106CFU/mL, viable count of Bifidobacterium 1 × 106CFU/mL, viable count of lactobacillus 2x106CFU/mL, viable count of Lactobacillus acidophilus 3x108CFU/mL, viable count of Lactobacillus casei 6x106CFU/mL, photosynthetic bacteria viable count of 9x106CFU/mL, viable count of actinomycete 9x107CFU/mL, the number of viable bacteria of Clostridium butyricum is 5x108CFU/mL, the viable count of the bacillus coagulans is 8x109CFU/mL。
And (5) microbial inoculum: the viable count of the bacillus subtilis is 1x109CFU/mL, the number of live bacteria of the saccharomyces cerevisiae is 8x106CFU/mL, Clostridium butyricum viable count of 9x108CFU/mL, viable count of Lactobacillus plantarum 5x106CFU/mL, the viable count of Pediococcus pentosaceus is2x106CFU/mL, viable count of enterococcus faecalis 1x108CFU/mL, viable count of Acetobacter 7x108CFU/mL, viable count of Bifidobacterium 5 × 109CFU/mL, viable count of lactobacillus 6x108CFU/mL, viable count of Lactobacillus acidophilus 8x1010CFU/mL, viable count of Lactobacillus casei 1x104CFU/mL, photosynthetic bacteria viable count of 1x104CFU/mL, viable count of actinomycete 2x105CFU/mL, the number of viable bacteria of Clostridium butyricum is 5x106CFU/mL, viable count of Bacillus coagulans of 1x106CFU/mL。
And (6) microbial inoculum: the difference from the microbial inoculum 1 is that the microbial inoculum does not contain bifidobacteria;
and (7) microbial inoculum: the difference from the microbial inoculum 1 is that the lactobacillus acidophilus is not contained;
and (4) microbial inoculum 8: the difference from the microbial inoculum 1 is that the microbial inoculum does not contain clostridium butyricum;
and (2) microbial inoculum 9: the difference from the microbial inoculum 1 is that the bacillus coagulans is not contained;
10, microbial inoculum: the difference from the microbial inoculum 1 is that the bacillus subtilis is not contained.
Example 3 application of microbial modifier in poultry or livestock feeding
In this embodiment, a pig is taken as an example, and other domestic fowls or animals such as chicken, duck, goose, pigeon, partridge, quail, cattle, sheep, etc. are used in the same manner as in this embodiment.
1. Application of microbial modifying microbial inoculum in healthy pigs
1100 weaned piglets with similar body types and states are selected and randomly divided into 11 groups of 100 piglets, wherein ten groups are set as drinking probiotics groups: comparing the ratio of the microbial improved liquid inoculum 1-10 prepared in the example 2 to water 1: after being diluted by 600 times, the pigs are allowed to drink water daily. The other group is a control group, and the other group is not added with the microorganism modified liquid microbial inoculum and is changed to drink equal amount of water. The average value of each index of each group was calculated after continuous feeding for 42 days, and the statistical results are shown in table 1.
TABLE 1
Research shows that after the liquid microbial inoculum is modified by microorganisms, the growth and the propagation of harmful bacteria such as escherichia coli, salmonella and the like can be effectively inhibited, the growth of beneficial bacteria such as lactobacillus, bifidobacterium and the like is promoted, the balance of intestinal flora of pigs is maintained, the intestinal health of the pigs is promoted, the immunity of the pigs is enhanced, and the disease resistance and the stress resistance are improved. Meanwhile, beneficial bacteria synthesize nutrients such as B vitamins, amino acids and the like in the pig body, vitamin deficiency is prevented, the microbial inoculum can provide partial nutrients required by the fattening pig, the early slaughter of the fattening pig is shortened, the hair color is smooth, the fur is ruddy, and the digestion and utilization rate of feed nutrients is improved. The experimental results in table 1 show that, compared with the control group, the average material consumption is remarkably reduced after the microbial improved liquid microbial inoculum 1-5 is used, the daily gain is increased, the feed conversion ratio is reduced, and the death and elutriation number is also remarkably reduced. Moreover, the effect of the microbial inoculum 1-5 is obviously better than that of the microbial inoculum 6-7, which shows that the effect of the microbial inoculum is reduced due to the interaction among all the floras and the lack of any floras.
2. Application of microbial modifying microbial inoculum in sick and weak pigs
The following four pig diseases are subjected to comparative experiments by using the microbial improvement fungicide 1 and common antibiotic products sold in the market: subject: the sick pigs of 40 days old are divided into 4 comparison groups of diarrhea disease, edema disease, swine flu and swine plague, each group of disease is divided into an AB group, a group A is used (microbial improvement fungicide 1), and a group B is used (common antibiotic medicine group). The AB groups are respectively 10 sick pigs, and the disease conditions are as follows: day one, group a used dilution 1: 200 times of the product for direct drinking, comprising 1: spraying 100 times of the feed directly to a pigsty once a day; the B group was administered with the same amount of the drugs, and the change of the disease and the time were recorded.
The results show that the group A has improvement after drinking for 1-5 days and basically recovers after 6-10 days in the diarrhea group; in the group B, 8 patients recover in 1-20 days, and 2 patients die;
in the edema group, the group A recovers 1 to 12 days; in the group B, 9 patients recover in 1-23 days, and 1 patient dies;
in the swine influenza group, the group A recovers in 1-15 days; group B basically improves 7 heads in 1-25 days, and dies 3 heads;
in the swine plague group, the group A recovers 1-20 days; in group B, 7 subjects in 1-30 days are basically improved, and 3 subjects die.
From the results, the microbial inoculum of the invention can obviously improve the physical state of sick and weak pigs and shorten the recovery time of the sick and weak pigs.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (10)
1. A microbial improver, wherein said microbial improver comprises: bacillus subtilis, saccharomyces cerevisiae, clostridium butyricum, lactobacillus plantarum, pediococcus pentosaceus, enterococcus faecalis, bacillus aceticus, bifidobacterium, lactobacillus acidophilus, lactobacillus casei, photosynthetic bacteria, actinomycetes, clostridium butyricum and bacillus coagulans.
2. Microbial improver according to claim 1, characterized in that the total number of viable colonies of said microbial improver is not less than 1x109CFU/mL。
3. A microbial improver according to claim 1, wherein in said microbial improver: the viable count of the bacillus subtilis is 1x109—8x1010CFU/mL, the number of live Saccharomyces cerevisiae is 3x104—8x106CFU/mL, Clostridium butyricum viable count of 2x106—9x108CFU/mL, viable count of Lactobacillus plantarum 1x104—5x106CFU/mL, viable count of Pediococcus pentosaceus 2X106—5x108CFU/mL, viable count of enterococcus faecalis 2x106—1x108CFU/mL, viable count of acetobacter 3x106—7x108CFU/mL, viable count of Bifidobacterium 1 × 106—5x109CFU/mL, viable count of lactobacillus 2x106—6x108CFU/mL, viable count of Lactobacillus acidophilus 3x108—8x1010CFU/mL, viable count of Lactobacillus casei 1x104—6x106CFU/mL, photosynthetic bacteria viable count of 1x104—9x106CFU/mL, viable count of actinomycete 2x105—9x107CFU/mL, the number of viable bacteria of Clostridium butyricum is 5x106—5x108CFU/mL, viable count of Bacillus coagulans of 1x106—8x109CFU/mL。
4. A microbial improver according to claim 3, wherein in said microbial improver: the viable count of the bacillus subtilis is 5x109—2x1010CFU/mL, the number of live Saccharomyces cerevisiae 5x105—2x106CFU/mL, Clostridium butyricum viable count of 8x107—5x108CFU/mL, viable count of Lactobacillus plantarum 5x105—1x106CFU/mL, viable count of Pediococcus pentosaceus 3X106—9x107CFU/mL, viable count of enterococcus faecalis 8x106—1x107CFU/mL, viable count of Acetobacter 6x106—9x107CFU/mL, viable count of Bifidobacterium 5 × 107—1x109CFU/mL, viable count of lactobacillus 8x107—1x108CFU/mL, viable count of Lactobacillus acidophilus 5x109—1x1010CFU/mL, viable count of Lactobacillus casei 5x104—1x106CFU/mL, 6x10 photosynthetic bacteria viable count5—1x106CFU/mL, viable count of actinomycete 1x106—5x107CFU/mL, the number of viable bacteria of Clostridium butyricum is 1x107—1x108CFU/mL, viable count of Bacillus coagulans of 1x107—5x109CFU/mL。
5. A microbial improver according to claim 4, wherein in said microbial improver: the viable count of the bacillus subtilis is 8x109CFU/mL, the number of live bacteria of saccharomyces cerevisiae is 1x106CFU/mL, Clostridium butyricum viable count of 2x108CFU/mL, viable count of Lactobacillus plantarum 7x105CFU/mL, viable count of Pediococcus pentosaceus 2X107CFU/mL, viable count of enterococcus faecalis 9x106CFU/mL, viable count of Acetobacter 2x107CFU/mL, viable count of Bifidobacterium 4x108CFU/mL, viable count of lactobacillus 9x107CFU/mL, viable count of Lactobacillus acidophilus 7x109CFU/mL, viable count of Lactobacillus casei 5x105CFU/mL, photosynthetic bacteria viable count of 8x105CFU/mL, viable count of actinomycete 3x107CFU/mL, the number of viable bacteria of Clostridium butyricum is 8x107CFU/mL, the viable count of the bacillus coagulans is 6x108CFU/mL。
6. A process for the preparation of a microbial improver according to any one of claims 1 to 5, comprising the steps of:
(1) solid slant culture: respectively inoculating bacillus subtilis, saccharomyces cerevisiae, clostridium butyricum, lactobacillus plantarum, pediococcus pentosaceus, enterococcus faecalis, bacillus aceticus, bifidobacterium, lactobacillus acidophilus, lactobacillus casei, photosynthetic bacteria, actinomycetes, clostridium butyricum and bacillus coagulans and culturing the inoculated bacillus subtilis, the saccharomyces cerevisiae, the clostridium butyricum, the lactobacillus plantarum, the pediococcus pentosaceus, the lactobacillus casei, the photosynthetic bacteria, the actinomycetes, the clostridium butyricum and the bacillus coagulans with a solid slant culture medium to fully activate strains;
(2) inoculating the activated bacillus subtilis, saccharomyces cerevisiae, clostridium butyricum, lactobacillus plantarum, pediococcus pentosaceus, enterococcus faecalis, bacillus aceticus, bifidobacterium, lactobacillus acidophilus, lactobacillus casei, photosynthetic bacteria, actinomycetes, clostridium butyricum and bacillus coagulans in the step (1) into a sterile liquid culture medium for mixed culture to obtain a total mixed flora;
(3) inoculating the total mixed flora in the step (2) to a fermentation culture medium by 5% of volume, performing fermentation culture for 15-18 days at the temperature of 25-30 ℃, continuously shaking or stirring in the fermentation culture process to obtain a bacterial liquid, centrifuging, concentrating, and washing the thalli with distilled water at normal temperature to obtain the microbial modifier.
7. The method of claim 6, wherein the total mixed population of Bacillus subtilis, Saccharomyces cerevisiae, Clostridium butyricum, Lactobacillus plantarum, Pediococcus pentosaceus, enterococcus faecalis, Acetobacter, Bifidobacterium, Lactobacillus acidophilus, Lactobacillus casei, photosynthetic bacteria, Actinomycetes, Clostridium butyricum, and Bacillus coagulans is present in a volume ratio of (20-40): 1-20): 1-10): 1-15): (1-20):(1-15):(1-30):(1-20):(5-30):(1-20):(1-25):(1-15):(5-20):(5-20).
8. The method of claim 6, wherein the solid slant medium comprises tryptone 15g, beef extract 8g, glucose 20g, sodium chloride 0.5g, distilled water make up 1000mL, agar 10g, pH7.4, sterilization conditions: 30min at 121 ℃; the formula of the liquid culture medium comprises: 15g of peptone, 8g of beef extract, 20g of glucose, 0.5g of sodium chloride, 1000mL of distilled water, pH7.4, and sterilization conditions: 30min at 121 ℃; the fermentation medium comprises peptone 20g/L, beef extract 10g/L, yeast 1g/L, glucose 5g/L, fructo-oligosaccharide 5g/L, pH7.2, and sterilization conditions are as follows: 121 ℃ and 30 min.
9. Use of a microbial modifier according to any one of claims 1 to 5 in poultry or livestock breeding.
10. Use according to claim 9, wherein the microbial improver is used in poultry or livestock breeding by mixing the microbial improver in a ratio of 1: diluting with water at the ratio of 200-: 300-500 times of the feed is diluted by water and mixed with feed for feeding.
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Application publication date: 20201023 |