CN115322925A - Liquid state fermentation complete feed for pigs and preparation method and application thereof - Google Patents
Liquid state fermentation complete feed for pigs and preparation method and application thereof Download PDFInfo
- Publication number
- CN115322925A CN115322925A CN202210870317.5A CN202210870317A CN115322925A CN 115322925 A CN115322925 A CN 115322925A CN 202210870317 A CN202210870317 A CN 202210870317A CN 115322925 A CN115322925 A CN 115322925A
- Authority
- CN
- China
- Prior art keywords
- liquid
- feed
- percent
- lactobacillus plantarum
- cfu
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000007788 liquid Substances 0.000 title claims abstract description 110
- 241000282887 Suidae Species 0.000 title claims abstract description 38
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 238000000855 fermentation Methods 0.000 title abstract description 33
- 230000004151 fermentation Effects 0.000 title abstract description 33
- 241000894006 Bacteria Species 0.000 claims abstract description 38
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 32
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 32
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 32
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 31
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 31
- 241000193749 Bacillus coagulans Species 0.000 claims abstract description 30
- 241000191996 Pediococcus pentosaceus Species 0.000 claims abstract description 30
- 229940054340 bacillus coagulans Drugs 0.000 claims abstract description 30
- 230000001580 bacterial effect Effects 0.000 claims abstract description 25
- 239000000843 powder Substances 0.000 claims description 25
- 238000002156 mixing Methods 0.000 claims description 20
- 239000002131 composite material Substances 0.000 claims description 15
- 239000001963 growth medium Substances 0.000 claims description 14
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 12
- 241000282898 Sus scrofa Species 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 239000002068 microbial inoculum Substances 0.000 claims description 11
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 9
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 9
- 238000012258 culturing Methods 0.000 claims description 9
- 238000009630 liquid culture Methods 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 9
- 244000068988 Glycine max Species 0.000 claims description 6
- 235000010469 Glycine max Nutrition 0.000 claims description 6
- 235000019764 Soybean Meal Nutrition 0.000 claims description 6
- 240000008042 Zea mays Species 0.000 claims description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 6
- 235000005822 corn Nutrition 0.000 claims description 6
- 239000004455 soybean meal Substances 0.000 claims description 6
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 4
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims description 4
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims description 4
- 239000012880 LB liquid culture medium Substances 0.000 claims description 4
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims description 4
- 239000004473 Threonine Substances 0.000 claims description 4
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims description 4
- XAHQYEAIJGTPET-JEDNCBNOSA-N [(1s)-5-amino-1-carboxypentyl]azanium;dihydrogen phosphate Chemical compound OP(O)([O-])=O.NCCCC[C@H]([NH3+])C(O)=O XAHQYEAIJGTPET-JEDNCBNOSA-N 0.000 claims description 4
- 230000003213 activating effect Effects 0.000 claims description 4
- 239000011575 calcium Substances 0.000 claims description 4
- 229910052791 calcium Inorganic materials 0.000 claims description 4
- 229930182817 methionine Natural products 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 239000004575 stone Substances 0.000 claims description 4
- 230000003321 amplification Effects 0.000 claims description 3
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 3
- 239000004480 active ingredient Substances 0.000 claims description 2
- 230000008014 freezing Effects 0.000 claims description 2
- 238000007710 freezing Methods 0.000 claims description 2
- 239000002054 inoculum Substances 0.000 claims description 2
- 230000000813 microbial effect Effects 0.000 claims description 2
- 238000009313 farming Methods 0.000 claims 1
- 238000003860 storage Methods 0.000 claims 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 abstract description 12
- 230000000433 anti-nutritional effect Effects 0.000 abstract description 8
- 235000016709 nutrition Nutrition 0.000 abstract description 8
- 239000004310 lactic acid Substances 0.000 abstract description 6
- 235000014655 lactic acid Nutrition 0.000 abstract description 6
- 235000014113 dietary fatty acids Nutrition 0.000 abstract description 5
- 229930195729 fatty acid Natural products 0.000 abstract description 5
- 239000000194 fatty acid Substances 0.000 abstract description 5
- 150000004665 fatty acids Chemical class 0.000 abstract description 5
- 244000052616 bacterial pathogen Species 0.000 abstract description 4
- 235000019629 palatability Nutrition 0.000 abstract description 4
- 239000002253 acid Substances 0.000 abstract description 3
- 230000000968 intestinal effect Effects 0.000 abstract description 3
- 239000003205 fragrance Substances 0.000 abstract 1
- SFAYBQDGCKZKMH-UHFFFAOYSA-N BNCC Chemical compound BNCC SFAYBQDGCKZKMH-UHFFFAOYSA-N 0.000 description 11
- 238000005457 optimization Methods 0.000 description 9
- 241000193830 Bacillus <bacterium> Species 0.000 description 7
- 241000588724 Escherichia coli Species 0.000 description 6
- 241000186660 Lactobacillus Species 0.000 description 6
- 241000607142 Salmonella Species 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 229940039696 lactobacillus Drugs 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 239000008176 lyophilized powder Substances 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- 241000194108 Bacillus licheniformis Species 0.000 description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- 230000004913 activation Effects 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 239000000835 fiber Substances 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 235000019750 Crude protein Nutrition 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 230000036528 appetite Effects 0.000 description 3
- 235000019789 appetite Nutrition 0.000 description 3
- 235000015278 beef Nutrition 0.000 description 3
- 239000000306 component Substances 0.000 description 3
- 230000002354 daily effect Effects 0.000 description 3
- 239000003599 detergent Substances 0.000 description 3
- 235000019621 digestibility Nutrition 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 210000001035 gastrointestinal tract Anatomy 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 230000007480 spreading Effects 0.000 description 3
- 238000003892 spreading Methods 0.000 description 3
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 description 2
- 108010011619 6-Phytase Proteins 0.000 description 2
- 235000019743 Choline chloride Nutrition 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 101710162629 Trypsin inhibitor Proteins 0.000 description 2
- 229940122618 Trypsin inhibitor Drugs 0.000 description 2
- 229910021536 Zeolite Inorganic materials 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 229960003178 choline chloride Drugs 0.000 description 2
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000005138 cryopreservation Methods 0.000 description 2
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 2
- 235000021050 feed intake Nutrition 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 108010083391 glycinin Proteins 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 239000013028 medium composition Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 229940085127 phytase Drugs 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 239000002753 trypsin inhibitor Substances 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 239000010457 zeolite Substances 0.000 description 2
- OBMBUODDCOAJQP-UHFFFAOYSA-N 2-chloro-4-phenylquinoline Chemical compound C=12C=CC=CC2=NC(Cl)=CC=1C1=CC=CC=C1 OBMBUODDCOAJQP-UHFFFAOYSA-N 0.000 description 1
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- 208000037260 Atherosclerotic Plaque Diseases 0.000 description 1
- 238000008157 ELISA kit Methods 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 108010088395 Glycine max alpha-conglycinin Proteins 0.000 description 1
- 108700037728 Glycine max beta-conglycinin Proteins 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000002535 acidifier Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 235000014590 basal diet Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 239000003640 drug residue Substances 0.000 description 1
- 238000005485 electric heating Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000036449 good health Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000006872 mrs medium Substances 0.000 description 1
- 239000006916 nutrient agar Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 150000002972 pentoses Chemical class 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 description 1
- 239000001393 triammonium citrate Substances 0.000 description 1
- 235000011046 triammonium citrate Nutrition 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/41—Pediococcus
- A23V2400/427—Pentosaceus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/25—Lactobacillus plantarum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention provides a liquid fermentation complete feed for pigs and a preparation method and application thereof. The strain formula used for fermentation comprises lactobacillus plantarum, pediococcus pentosaceus, bacillus subtilis and bacillus coagulans, and the bacterium liquids are mixed according to the ratio of 1; the feed used for fermentation is complete feed, and the addition amount of the bacterial liquid is 6-8%. The liquid fermented feed provided by the invention can improve the nutritional value of the feed, reduce anti-nutritional factors, generate lactic acid and various volatile fatty acids and inhibit the growth of intestinal pathogenic bacteria; the liquid fermented feed has good palatability, has a certain acid fragrance, and can improve the growth performance of growing pigs.
Description
Technical Field
The invention relates to the technical field of breeding feed, in particular to liquid fermentation complete feed for pigs and a preparation method and application thereof.
Background
The soybean meal and the corn account for about 70 percent of the global feed consumption, but the nutritional value of the soybean meal and the corn is limited by anti-nutritional factors, so that huge economic losses are caused to the animal husbandry production. The shortage of feed resources causes the price of the conventional feed raw materials to greatly rise, and the fermentation technical means can improve the utilization rate of the nutrient value of the feed, thereby reducing the cost of the feed. The abuse of antibiotics causes high drug residue in animal products, the generated super bacteria seriously endangers the life safety of human beings and animals, and the search for novel safe and antibiotic-free biological feed becomes urgent along with the proposal of feed resistance policy.
The liquid fermented feed is a liquid fermented feed developed by using a microbial fermentation engineering in a biological feed, and is widely researched due to the benefits that the liquid fermented feed can relieve the weaning stress of piglets and increase the biological utilization rate and the nutritional value.
The preparation method and application of the liquid fermented feed for growing pigs need to be comprehensively and deeply researched systematically.
Disclosure of Invention
The invention aims to provide a liquid fermented complete feed for pigs and a preparation method and application thereof.
In order to achieve the object, in a first aspect, the invention provides a complex microbial inoculum, and the active ingredients are Lactobacillus plantarum (Lactobacillus plantarum), pediococcus pentosaceus (Pediococcus pentosaceus), bacillus subtilis (Bacillus subtilis) and Bacillus coagulans (Bacillus coagulons).
Preferably, the lactobacillus plantarum is deposited under accession number BNCC336421, the pediococcus pentosaceus is deposited under accession number BNCC135034, the bacillus subtilis is deposited under accession number BNCC109047, and the bacillus coagulans is deposited under accession number BNCC337209.
The viable count of the lactobacillus plantarum in the composite microbial inoculum is 3.7 multiplied by 10 9 ～4.1×10 9 CFU/mL, viable count of Pediococcus pentosaceus 4.1X 10 9 ～4.5×10 9 CFU/mL, viable count of Bacillus subtilis is 1.2 × 10 9 ～2×10 9 CFU/mL, viable count of Bacillus coagulans of 5.8 × 10 9 ～6.5×10 9 CFU/mL。
In a second aspect, the invention provides a preparation method of a complex microbial inoculum, which comprises the following steps:
(1) Activating strains: culturing the lactobacillus plantarum freeze-dried powder and the pediococcus pentosaceus freeze-dried powder for 18-24 h at 36-38 ℃ in an MRS culture medium; respectively culturing the bacillus subtilis freeze-dried powder and the bacillus coagulans freeze-dried powder for 18-24 h at 36-38 ℃ by using an LB liquid culture medium and a bacillus coagulans culture medium, selecting 1-2 bacterial colonies of each bacterium, storing the bacterial colonies in a cryopreservation tube by using a 20-30% glycerol solution, and storing the bacterial colonies in a refrigerator at-80 ℃;
(2) And (3) amplification culture: recovering the frozen bacteria liquid in the step (1), inoculating the bacteria liquid into a corresponding liquid culture medium, shaking up, and culturing for 18-24 h at the temperature of 36-38 ℃;
(3) Mixing bacterial liquid: and (3) uniformly mixing the lactobacillus plantarum activated bacterium liquid obtained in the step (2), the bacillus subtilis activated bacterium liquid, the pediococcus pentosaceus activated bacterium liquid and the bacillus coagulans activated bacterium liquid according to a volume ratio of 1.
In a third aspect, the invention provides an application of the complex microbial inoculum in preparation of fermented feed.
In a fourth aspect, the invention provides a liquid fermented complete feed for pigs, which is prepared by mixing and fermenting a pig feed and the complex microbial inoculum.
In a fifth aspect, the invention provides a preparation method of the liquid fermented complete feed for pigs, which comprises the following steps: mixing the pig feed with water according to a feed-liquid ratio of 1kg.
Preferably, the pig feed comprises: 61.73 percent of corn, 20.87 percent of puffed soybean, 11.02 percent of soybean meal, 2.07 percent of calcium hydrophosphate, 0.34 percent of stone powder, 0.67 percent of sodium chloride, 0.3 percent of citric acid, 0.5 percent of lysine phosphate, 0.22 percent of methionine, 0.2 percent of threonine, 0.08 percent of tryptophan and 2 percent of premix.
Wherein the premix provides 1.5mg of VC, 0.5kg of phytase, 500mg of antioxidant, 40.0g of zeolite powder, 41.5g of rice hull powder, 4.5mg of choline chloride (50%), 1.5g of 0.03% composite multi-vitamins (VA, VD, VE, VK and VB) and 10.0g of 0.2% trace elements (Zn, fe, cu, mn and the like) for each kilogram of feed.
In a sixth aspect, the invention provides application of the liquid fermented complete feed for pigs in pig breeding.
In the application, the liquid fermented complete feed for pigs is adopted to replace 10-15% of complete feed for feeding.
By the technical scheme, the invention at least has the following advantages and beneficial effects:
the invention provides a convenient and rapid method for growing liquid fermented feed for pigs, firstly, complete feed is selected in the aspect of feed, the complicated steps for preparing fermentation substrate are reduced, and the method is more beneficial to actual operation; secondly, in the aspect of strain selection, the invention provides a mode for activating the cryopreserved bacteria to ferment the liquid feed, wherein the lyophilized powder is high in price, a plurality of tubes of the lyophilized powder can be stored after one-time use, the activated bacteria liquid can be obtained in a mode of recovering the cryopreserved bacteria in the next batch of fermentation, the recovery time is generally 1-2 min, and the labor, material and financial resources can be saved.
The liquid fermented feed provided by the invention can improve the nutritional value of the feed, reduce the anti-nutritional factors of the feed, generate lactic acid and various volatile fatty acids, inhibit the activity of escherichia coli and salmonella in intestinal tracts, and improve the quality of the feed. The liquid fermented feed provided by the invention can also improve the growth performance of growing pigs, has unique acid flavor and good palatability, can promote the appetite of the pigs and increase the feed intake.
Drawings
FIG. 1 is a graph showing the inhibitory effect of fermentation broth on Escherichia coli and Salmonella in a preferred embodiment of the present invention. Wherein A is an Escherichia coli plate, and B is a Salmonella plate.
Detailed Description
The liquid fermented complete feed for the growing pigs can meet the nutritional requirements of the growing pigs, is good in palatability, generates lactic acid and various volatile fatty acids, inhibits the growth and the reproduction of intestinal pathogenic bacteria, and improves the growth performance of the growing pigs.
The invention adopts the following technical scheme:
the invention provides a compound bacterial liquid of liquid fermentation complete feed for growing pigs, which comprises lactobacillus plantarum activated bacterial liquid, pediococcus pentosaceus activated bacterial liquid, bacillus subtilis activated bacterial liquid and bacillus coagulans activated bacterial liquid.
The complex bacterial liquid for fermenting liquid feed as described above, preferably, the lactobacillus plantarum is deposited under accession number BNCC336421, pediococcus pentosaceus is deposited under accession number BNCC135034, the bacillus subtilis is deposited under accession number BNCC109047, and the bacillus coagulans is deposited under accession number BNCC337209.
The composite bacterial liquid for fermenting liquid feed as described above preferably has a mixing volume part ratio of lactobacillus plantarum, bacillus subtilis, pediococcus pentosaceus and bacillus coagulans of 1.
Preferably, the viable count of the lactobacillus plantarum solution before mixing is not less than 3.7 x 10 9 CFU/mL, viable count of the bacterial liquid of pentosaceus is not less than 4.1 × 10 9 CFU/mL, viable count in Bacillus subtilis solution is not less than 1.2 × 10 9 CFU/mL, viable count of Bacillus coagulans liquid is not less than 5.8 × 10 9 CFU/mL。
Preferably, the fermentation substrate: the fermentation substrate comprises the following components by taking the total mass of the components of the fermentation substrate as 100 percent: 61.73 percent of corn, 20.87 percent of expanded soybean, 11.02 percent of soybean meal, 2.07 percent of calcium hydrophosphate, 0.34 percent of stone powder, 0.67 percent of sodium chloride, 0.3 percent of acidifier, 0.5 percent of lysine phosphate, 0.22 percent of methionine, 0.2 percent of threonine, 0.08 percent of tryptophan and 2 percent of premix.
The invention also provides a liquid fermented complete feed for growing pigs, which is a liquid feed obtained by fermenting the complete feed by adopting the composite bacterial liquid.
The preparation method of the liquid fermented feed for growing pigs comprises the following steps:
(1) Preparing the composite bacterial liquid for fermenting the liquid feed;
(2) Sterilizing the mixed feed before water extraction, mixing the sterilized mixed feed with complete feed, and inoculating 6 percent (mL/g) of the composite bacterial liquid prepared in the step (1) into a mixture of the feed and the water;
(3) After being mixed evenly, the sealing bag is sealed and is fermented for 3 to 5 days under the condition of 30 ℃.
Preferably, in the step (1), the lactobacillus plantarum freeze-dried powder and the pediococcus pentosaceus freeze-dried powder are cultured for 24 hours at 37 ℃ in MRS medium; respectively culturing the bacillus subtilis freeze-dried powder and the bacillus coagulans freeze-dried powder for 24h at 37 ℃ by using an LB liquid culture medium and a bacillus coagulans culture medium, selecting 1-2 colonies after the culture is finished, mixing the colonies in a freezing tube by using a 20% sterilized glycerol solution according to a ratio of 1.
Preferably, in the step (1), the preparation of the composite bacterial liquid for fermenting liquid feed as described above is performed by recovering cryopreserved bacteria of lactobacillus plantarum and pediococcus pentosaceus, and then respectively culturing the cryopreserved bacteria of lactobacillus plantarum and pediococcus pentosaceus by using MRS liquid culture medium based on a 37 ℃ shaking constant temperature incubator to obtain lactobacillus plantarum activated bacterial liquid and pediococcus pentosaceus activated bacterial liquid; recovering cryopreserved bacteria of bacillus subtilis and bacillus coagulans, and then respectively culturing by using an LB liquid culture medium and a coagulation liquid culture medium in a 37 ℃ oscillation constant-temperature incubator to obtain bacillus subtilis activated bacteria liquid and bacillus coagulans activated bacteria liquid; the mixing volume part ratio of the obtained lactobacillus plantarum activated bacterium liquid, the obtained bacillus subtilis activated bacterium liquid, the obtained pediococcus pentosaceus activated bacterium liquid and the obtained bacillus coagulans activated bacterium liquid is 1.
Preferably, in step (2), the ratio of the complete feed to water is 1.
The liquid fermentation complete feed is applied to the cultivation of growing pigs, and preferably 10% of complete feed is replaced by the liquid fermentation complete feed for feeding.
The following examples are intended to illustrate the invention, but are not intended to limit the scope of the invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are commercially available products.
The percent in the present invention means mass percent unless otherwise specified; but the percent of the solution, unless otherwise specified, refers to the grams of solute contained in 100mL of the solution.
EXAMPLE 1 optimization of fermentation Strain and Process
Lactobacillus plantarum having accession number BNCC336421, pediococcus pentosaceus having accession number BNCC135034, bacillus subtilis having accession number BNCC109047, and Bacillus coagulans having accession number BNCC337209 are all model strains of the present invention, which were purchased from the BNCC culture collection. BNCC336463 Bacillus licheniformis was purchased from North Nah Biotechnology Ltd.
MRS liquid culture medium, beef extract, peptone, agar, yeast powder and other reagents are purchased from Solibao. The growing pig feed is purchased from Jinzhong Shiyang feed Co., ltd, and mainly comprises the following components: 61.73 percent of corn, 20.87 percent of puffed soybean, 11.02 percent of soybean meal, 2.07 percent of calcium hydrophosphate, 0.34 percent of stone powder, 0.67 percent of sodium chloride, 0.3 percent of citric acid, 0.5 percent of lysine phosphate, 0.22 percent of methionine, 0.2 percent of threonine, 0.08 percent of tryptophan and 2 percent of premix; the premix provides 1.5mg of VC, 0.5kg of phytase, 500mg of antioxidant, 40.0g of zeolite powder, 41.5g of rice hull powder, 4.5mg of choline chloride (50%), 1.5g of 0.03% composite multi-vitamin (VA, VD, VE, VK and VB) and 10.0g of 0.2% trace elements (Zn, fe, cu, mn and the like) for each kilogram of feed.
(1) Strain activation
a. Activation of lactobacillus plantarum: the lactobacillus plantarum (BNCC 336421) freeze-dried powder is fully dissolved and uniformly mixed with 0.5mL of MRS liquid culture medium (10.0 g of peptone, 10.0g of beef extract, 4.0g of yeast powder, 20.0g of glucose, 0.2g of magnesium sulfate, 5.0g of sodium acetate, 2.0g of triammonium citrate, 2.0g of dipotassium hydrogen phosphate, 0.04g of manganese sulfate, 80.1g of Tween and 1.0L of distilled water). Then 0.2mL of the suspension was pipetted onto a solid medium (20.0 g agar based on the liquid medium components) plate and spread evenly with a spreading bar. The plate was incubated at 37 ℃ in a constant temperature incubator for 24h.
b. Activation of pediococcus pentosaceus: the lyophilized powder of pediococcus pentosaceus (BNCC 109047) is fully dissolved by 0.5mL of MRS liquid culture medium and evenly mixed. Then 0.2mL of the bacterial suspension is sucked and injected into a solid medium flat plate, and the solid medium flat plate is evenly coated by a coating rod. The plate was incubated at 37 ℃ in a constant temperature incubator for 24h.
c. Activating bacillus subtilis and bacillus licheniformis: the Bacillus subtilis (BNCC 135034) lyophilized powder or Bacillus licheniformis (BNCC 336463) lyophilized powder was dissolved in 0.5mL nutrient agar liquid medium (beef extract 3.0g, peptone 10.0g, naCl 5.0g, distilled water 1.0L) and mixed well. Then 0.2mL of the suspension was pipetted onto a solid medium (20.0 g agar based on the liquid medium composition) plate and spread evenly with a spreading bar. The plate was incubated at 37 ℃ in a constant temperature incubator for 24h.
d. Activation of bacillus coagulans: fully dissolving the freeze-dried powder of the bacillus coagulans (BNCC 337209) in 0.5mL of liquid culture medium (10.0 g of polypeptone, 2.0g of yeast powder, 1.0g of magnesium sulfate and 1.0L of distilled water) and uniformly mixing. Then 0.4mL of the suspension was pipetted onto a solid medium (15.0 g agar based on the liquid medium composition) plate and spread evenly with a spreading bar. The plate was incubated at 37 ℃ in a constant temperature incubator for 24h.
(2) The strain is frozen and recovered for use: 1-2 colonies were picked from the plate and washed with a suitable amount of 20% sterile glycerol solution into a 1.5mL cryopreservation tube, sealed with a sealing membrane and cryopreserved at-80 ℃. Taking a tube of each strain before each fermentation, and quickly melting the strain in a water bath for 1-2 min. After thawing, respectively adding the 5 resuscitation bacteria liquid into respective liquid culture medium, uniformly mixing, and placing into a shaking incubator at 37 ℃ for amplification culture for 24h.
(3) Preference of the fermenting species
The method comprises the following steps of combining 5 kinds of bacteria in different ways, preparing fermented feed according to a primary fermentation process, mixing the bacteria solutions according to the same fraction ratio to obtain a compound bacteria solution, wherein the feed used for fermentation is a complete feed, the feed-water ratio is 1. The test group 1 is a combination of lactobacillus plantarum and pediococcus pentosaceus; the test group 2 is a combination of lactobacillus plantarum, pediococcus pentosaceus and bacillus subtilis; the test group 3 is a combination of lactobacillus plantarum, pediococcus pentosaceus, bacillus subtilis and bacillus licheniformis; test group 4 was a combination of lactobacillus plantarum, pediococcus pentosaceus, bacillus subtilis, and bacillus coagulans.
TABLE 1 optimization of the fermentation Strain
Note: the data in the same row is marked with no letters or the same lower case letters to indicate that the difference is not significant (P > 0.05), different lower case letters to indicate that the difference is significant (P < 0.05), and different upper case letters to indicate that the difference is extremely significant (P < 0.01).
As can be seen from table 1, the test group 4 had the lowest pH value and the highest lactic acid content, and the remaining volatile fatty acid content was significantly higher than each group, and thus, it was finally determined that the fermenting species were a combination of lactobacillus plantarum, pediococcus pentosaceus, bacillus subtilis, and bacillus coagulans.
(4) Optimization of fermentation strain proportion
The screened four bacteria are divided into bacillus and lactobacillus, fermented feed is prepared according to a preliminary fermentation process, and is mixed according to the proportion of 1.
TABLE 2 optimization of the proportion of fermentation strains
Note: the same row data shoulder marks have no letters or the same lower case letters indicate no significant difference (P > 0.05), different lower case letters indicate significant difference (P < 0.05), and different upper case letters indicate significant difference (P < 0.01).
As can be seen from table 2, the fermented feed prepared by mixing the number of bacillus and the number of lactobacillus at a ratio of 1 is the highest in the number of bacillus and lactobacillus, and therefore, it is appropriate to select a ratio of 1.
(5) Proportion optimization of composite microbial inoculum and feed
The four bacteria are mixed according to the proportion of 1.
TABLE 3 optimization of the ratio of complex inoculant to feed
Note: the same row data shoulder marks have no letters or the same lower case letters indicate no significant difference (P > 0.05), different lower case letters indicate significant difference (P < 0.05), and different upper case letters indicate significant difference (P < 0.01).
As can be seen from Table 3, when the amount of the bacterial liquid added is 6%, the number of Bacillus and Lactobacillus is the highest, and therefore, it is preferable to select 6% of the amount of the bacterial liquid added.
(6) Optimization of fermentation temperature
And (2) mixing the four bacteria according to the proportion of 1.
TABLE 4 optimization of fermentation temperature
Note: the same row data shoulder marks have no letters or the same lower case letters indicate no significant difference (P > 0.05), different lower case letters indicate significant difference (P < 0.05), and different upper case letters indicate significant difference (P < 0.01).
As can be seen from Table 4, the number of Bacillus and Lactobacillus was the highest at a constant incubator temperature of 30 ℃ and it was therefore preferable to select a fermentation temperature of 30 ℃.
(7) Preparation method of liquid fermented feed for pigs
Based on the optimization result, the preparation method of the liquid fermented feed comprises the following steps: and uniformly mixing the expanded plant lactobacillus liquid, the bacillus subtilis liquid, the pentosan spherule liquid and the condensed bacillus liquid according to the volume ratio of 1. Wherein the viable count of Lactobacillus plantarum is not less than 3.7 × 10 9 CFU/mL, pentose tabletsThe viable count of the spore bacteria liquid is not less than 4.1 × 10 9 CFU/mL, the number of viable bacteria of Bacillus subtilis liquid is not less than 1.2 × 10 9 CFU/mL, the number of viable bacteria of the bacillus coagulans liquid is not less than 5.8 multiplied by 10 through determination 9 CFU/mL。
Mixing the complete feed for growing pigs with sterilized water according to a ratio of 1kg; after being mixed evenly, the sealing bag is sealed, and the mixture is placed in a constant temperature electric heating incubator for fermentation at 30 ℃ for 3 to 5 days, thus obtaining the liquid fermented feed for growing pigs (liquid fermented complete feed for pigs).
Example 2 quality of liquid fermented feed for swine
The liquid fermented feed for growing pigs prepared in example 1 was compared with a general complete feed to verify the quality of the feed.
1. Material
The lactic acid kit and the total amino acid kit are purchased from Nanjing to build a bioengineering institute; escherichia coli (BNCC 337004) and Salmonella (BNCC 186354) were purchased from BNCC culture collection center; oxford cups were purchased from shanghai instrument, 32704m science and technology development ltd; ELISA kits such as glycinin, soybean trypsin inhibitor and the like are purchased from Jiangsu enzyme immunoassay limited company.
2. Method of producing a composite material
(1) Nutritional ingredients of liquid fermented feed
TABLE 5 nutritional ingredients of composite probiotic liquid fermented feed
Note: * P <0.05, P <0.01; the control group was a normal full rate diet.
As can be seen from Table 5, the contents of crude protein, organic matter and total amino acid in the fermented feed are all significantly higher than those in the control group (P < 0.01), but the contents of the acidic detergent fiber and the neutral detergent fiber are not significantly changed.
(2) Anti-nutritional factor content of liquid fermented feed
TABLE 6 Effect of liquid fermentation on antinutritional factor content in feed
Note: * P <0.05, P <0.01; the control group was a normal full rate ration.
As can be seen from table 6, the content of 4 anti-nutritional factors in the fermented feed group was significantly lower than that in the control group (P < 0.01), alpha-conglycinin was reduced by 17.08%, beta-conglycinin was reduced by 17.17%, glycinin was reduced by 31.95%, and soybean trypsin inhibitor was reduced by 15.41%.
(3) Bacteriostatic effect of liquid fermented feed fermentation liquor
TABLE 7 zone of inhibition diameter of fermentation broth against harmful pathogenic bacteria
Note: the control group was sterilized distilled water.
As can be seen from table 7 and fig. 1, the inhibition zone diameter of the fermentation broth group was significantly higher on both the escherichia coli (BNCC 337004) and salmonella (BNCC 186354) plates than the control group (P < 0.01).
The liquid fermented feed improves the nutritional value of the complete feed, reduces the anti-nutritional factor content of the feed, generates various lactic acid and volatile fatty acid, can inhibit the activity of intestinal pathogenic bacteria such as escherichia coli and salmonella, and improves the feed quality.
Example 3 application Effect of liquid fermented feed to growing pigs
In order to verify the application effect of the liquid fermented feed in example 1 to growing pigs, 24 big-sized hybrid pigs with good health condition, age of 75 +/-3 days and weight of 27.97 +/-4.48 kg are selected. The test group was fed with 10% of the liquid fermented feed prepared in example 1 mixed with 90% of the basal diet, and the form of the feed was liquid atheroma.
Each group of pigs was divided into three pens, each housed independently. All pigs had free access to water and food. Feeding and observing the residual material amount in the trough every morning and evening, weighing in advance and recording data in the next feed, thereby respectively determining the next feeding amount. The temperature, humidity and smell of the pigsty are observed every day, and the pigsty is ventilated at any time. The pre-feeding period is 5 days, the formal test is 30 days, the test is slaughtered, and the feeding results are as follows:
(1) Growth performance of growing pigs
TABLE 8 influence of complete feed and liquid fermented feed on growth performance of growing pigs
As can be seen from table 8, the average daily gain of the test group was significantly higher than that of the control group (P < 0.01), the feed-meat ratio was significantly lower than that of the control group (P < 0.01), and the end weight and average daily feed intake did not change significantly.
The liquid fermented feed can obviously improve the average daily gain of growing pigs, probably the acid flavor and good palatability generated after the feed is fermented can stimulate the appetite of the pigs, and meanwhile, the liquid fermented feed can reduce the acidity of the stomach in the stomach and also has the function of improving the appetite.
(2) Apparent digestibility of growing pigs
TABLE 9 Effect of liquid fermented feed on apparent digestibility
As can be seen from table 9, the crude protein digestibility of the test group was significantly higher than that of the control group (P < 0.01), and the neutral detergent fiber and the acid detergent fiber had a tendency to increase without significant difference.
The liquid fermented feed can improve the content of crude protein, part of the protein is mycoprotein and is easier to absorb, and meanwhile, part of anti-nutritional factors are removed, so that the burden of intestinal tracts can be relieved, and a group fed with the liquid fermented feed has more proteins which are easy to digest and absorb under the same feeding condition; meanwhile, the feed is rich in organic acid and bacteriostatic products, and can play a role in intestinal tracts to promote digestion.
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, it is intended that all such modifications and alterations be included within the scope of this invention as defined in the appended claims.
Claims (10)
1. The composite microbial inoculum is characterized in that the active ingredients are Lactobacillus plantarum (Lactobacillus plantarum), pediococcus pentosaceus (Pediococcus pentosaceus), bacillus subtilis (Bacillus subtilis) and Bacillus coagulans (Bacillus coagulans);
preferably, the lactobacillus plantarum is deposited under accession number BNCC336421, pediococcus pentosaceus is deposited under accession number BNCC135034, the bacillus subtilis is deposited under accession number BNCC109047, and the bacillus coagulans is deposited under accession number BNCC337209.
2. The complex microbial inoculum of claim 1, wherein the viable count of lactobacillus plantarum in the complex microbial inoculum is 3.7 x 10 9 ～4.1×10 9 CFU/mL, viable count of Pediococcus pentosaceus 4.1X 10 9 ～4.5×10 9 CFU/mL, viable count of Bacillus subtilis is 1.2 × 10 9 ～2×10 9 CFU/mL, viable count of Bacillus coagulans 5.8 × 10 9 ～6.5×10 9 CFU/mL。
3. The preparation method of the composite microbial inoculum is characterized by comprising the following steps:
(1) Activating strains: culturing the lactobacillus plantarum freeze-dried powder and the pediococcus pentosaceus freeze-dried powder for 18-24 h at 36-38 ℃ in an MRS culture medium; respectively culturing the bacillus subtilis freeze-dried powder and the bacillus coagulans freeze-dried powder for 18-24 h at 36-38 ℃ by using an LB liquid culture medium and a bacillus coagulans culture medium; after the culture is finished, 1-2 bacterial colonies are picked from each bacterium, preserved in a freezing storage tube by using a 20-30% glycerol solution and preserved in a refrigerator at the temperature of-80 ℃;
(2) And (3) amplification culture: recovering the frozen bacteria liquid in the step (1), inoculating the bacteria liquid into a corresponding liquid culture medium, shaking up, and culturing for 18-24 h at the temperature of 36-38 ℃;
(3) Mixing bacterial liquid: and (3) uniformly mixing the lactobacillus plantarum activated bacterium liquid, the bacillus subtilis activated bacterium liquid, the pediococcus pentosaceus activated bacterium liquid and the bacillus coagulans activated bacterium liquid obtained in the step (2) according to a volume ratio of 1.
4. The method as claimed in claim 3, wherein the viable count of Lactobacillus plantarum in the composite bacterial preparation is 3.7 x 10 9 ～4.1×10 9 CFU/mL, viable count of Pediococcus pentosaceus 4.1X 10 9 ～4.5×10 9 CFU/mL, viable count of Bacillus subtilis is 1.2 × 10 9 ～2×10 9 CFU/mL, viable count of Bacillus coagulans of 5.8 × 10 9 ～6.5×10 9 CFU/mL。
5. The use of the complex microbial inoculant of claim 1 or 2 in the preparation of fermented feed.
6. A liquid fermented complete feed for pigs, which is characterized in that the feed is prepared by mixing and fermenting the pig feed and the composite microbial inoculum according to claim 1 or 2.
7. The method for preparing a liquid fermented complete feed for pigs according to claim 6, wherein the method comprises: mixing the pig feed with water according to a feed-liquid ratio of 1kg.
8. The method of claim 7, wherein the pig feed comprises: 61.73 percent of corn, 20.87 percent of puffed soybean, 11.02 percent of soybean meal, 2.07 percent of calcium hydrophosphate, 0.34 percent of stone powder, 0.67 percent of sodium chloride, 0.3 percent of citric acid, 0.5 percent of lysine phosphate, 0.22 percent of methionine, 0.2 percent of threonine, 0.08 percent of tryptophan and 2 percent of premix.
9. The use of the liquid fermented complete feed for swine of claim 6 in swine farming.
10. The use according to claim 9, wherein the pig is fed with a liquid fermented complete feed instead of 10-15% complete feed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210870317.5A CN115322925B (en) | 2022-07-18 | 2022-07-18 | Liquid fermented complete feed for pigs and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210870317.5A CN115322925B (en) | 2022-07-18 | 2022-07-18 | Liquid fermented complete feed for pigs and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115322925A true CN115322925A (en) | 2022-11-11 |
| CN115322925B CN115322925B (en) | 2023-08-15 |
Family
ID=83918980
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210870317.5A Active CN115322925B (en) | 2022-07-18 | 2022-07-18 | Liquid fermented complete feed for pigs and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115322925B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115708544A (en) * | 2022-11-28 | 2023-02-24 | 四川德康农牧食品集团股份有限公司 | Fattening pig bacterial enzyme synergistic liquid state fermented feed and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20110033685A (en) * | 2009-09-25 | 2011-03-31 | 대한민국(농촌진흥청장) | Immunity reinforcement lactic acid bacterial strain and lactic acid bacterial preparation containing the same |
| CN106721278A (en) * | 2016-12-07 | 2017-05-31 | 天津牧丰饲料有限公司 | Microbial fermentation child care phase piglet liquid-state feed and preparation method and application |
| CN110506831A (en) * | 2019-07-17 | 2019-11-29 | 浙江大学 | Compoiste fermented feed and its preparation and application method for prevention of diarrhea in piglets |
| CN111466481A (en) * | 2020-04-13 | 2020-07-31 | 博益德（北京）生物科技有限公司 | Liquid bacterium solution for fermenting liquid piglet feed, preparation method and application of liquid fermented piglet feed |
| CN111802514A (en) * | 2020-07-07 | 2020-10-23 | 广东理想生物科学研究有限公司 | Microbial modifier and preparation method and application thereof |
| AU2021102073A4 (en) * | 2021-04-20 | 2021-06-03 | Institute of Animal Science, Guangdong Academy of Agricultural Sciences | Solid-state fermented feed for improving meat quality of fattening pigs as well as preparation method and application thereof |
-
2022
- 2022-07-18 CN CN202210870317.5A patent/CN115322925B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20110033685A (en) * | 2009-09-25 | 2011-03-31 | 대한민국(농촌진흥청장) | Immunity reinforcement lactic acid bacterial strain and lactic acid bacterial preparation containing the same |
| CN106721278A (en) * | 2016-12-07 | 2017-05-31 | 天津牧丰饲料有限公司 | Microbial fermentation child care phase piglet liquid-state feed and preparation method and application |
| CN110506831A (en) * | 2019-07-17 | 2019-11-29 | 浙江大学 | Compoiste fermented feed and its preparation and application method for prevention of diarrhea in piglets |
| CN111466481A (en) * | 2020-04-13 | 2020-07-31 | 博益德（北京）生物科技有限公司 | Liquid bacterium solution for fermenting liquid piglet feed, preparation method and application of liquid fermented piglet feed |
| CN111802514A (en) * | 2020-07-07 | 2020-10-23 | 广东理想生物科学研究有限公司 | Microbial modifier and preparation method and application thereof |
| AU2021102073A4 (en) * | 2021-04-20 | 2021-06-03 | Institute of Animal Science, Guangdong Academy of Agricultural Sciences | Solid-state fermented feed for improving meat quality of fattening pigs as well as preparation method and application thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115708544A (en) * | 2022-11-28 | 2023-02-24 | 四川德康农牧食品集团股份有限公司 | Fattening pig bacterial enzyme synergistic liquid state fermented feed and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115322925B (en) | 2023-08-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1813562A (en) | Compound microbial fermented fodder nutrient additive and its preparing method | |
| CN111436526A (en) | Preparation method and application of fermented rice bran meal with bacterium enzyme for improving growth performance of fattening pigs | |
| CN106974063B (en) | Method for producing high-efficiency protein feed by using feed enzyme in cooperation with bacillus coagulans | |
| CN113061558B (en) | Composite probiotics and feed additive containing bacillus coagulans HALO178 | |
| CN109198214A (en) | A kind of fermented feed, preparation method and its mixed feed | |
| CN115094012B (en) | Preparation method and application of bacillus coagulans BC-HYC strain microbial inoculum | |
| CN107312732A (en) | A kind of probiotic feed additive | |
| CN110178967A (en) | Fermented feed and its preparation method and application for improving meat quality | |
| CN108531411B (en) | Combined bacterium preparation for fermenting chicken manure | |
| CN115074290B (en) | Lactobacillus casei for co-production of phenyllactic acid and gamma-aminobutyric acid and application thereof | |
| CN112280707A (en) | Preparation method of probiotics for feed | |
| KR100541379B1 (en) | Animal feed additives and method of producing the same | |
| CN108277188B (en) | Compound microbial starter culture for weaned piglets and application thereof | |
| CN115322925B (en) | Liquid fermented complete feed for pigs and preparation method and application thereof | |
| CN108546663B (en) | Porcine lactobacillus crispatus and application thereof | |
| CN114365799A (en) | Growing and fattening pig feed capable of improving growth performance and preparation method thereof | |
| CN112375701A (en) | Microbial preparation for improving intestinal environment of ruminant such as cattle and sheep and preparation method thereof | |
| KR20120006601A (en) | Bacillus subtilis subsp. subtilis lsk 0604 in high-temperature type bacillus and making method for fish meal involving soybean meal thereof | |
| CN112088978A (en) | Lactic acid bacteria stabilizer and application method thereof | |
| CN114437975B (en) | Lactic acid-producing bacillus coagulans and application thereof | |
| CN115316496A (en) | Method for preparing high-protein probiotic animal feed by using waste feathers | |
| CN114381409A (en) | Fermented feed for improving animal production performance and/or improving feed utilization rate and application thereof | |
| CN113647518A (en) | Special biological fermentation feed for fattening cattle in northern Xinjiang area and preparation method thereof | |
| CN112790281A (en) | Preparation method of feed additive rich in surfactin and functional microorganisms | |
| CN111728081A (en) | Compound bacterium fermentation liquor for feed additive and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |