CN114381409A - Fermented feed for improving animal production performance and/or improving feed utilization rate and application thereof - Google Patents

Fermented feed for improving animal production performance and/or improving feed utilization rate and application thereof Download PDF

Info

Publication number
CN114381409A
CN114381409A CN202210170312.1A CN202210170312A CN114381409A CN 114381409 A CN114381409 A CN 114381409A CN 202210170312 A CN202210170312 A CN 202210170312A CN 114381409 A CN114381409 A CN 114381409A
Authority
CN
China
Prior art keywords
feed
parts
feed additive
microbial inoculum
corn
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN202210170312.1A
Other languages
Chinese (zh)
Other versions
CN114381409B (en
Inventor
刘晓兰
郑喜群
王松
王燕
韩国栋
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiqihar University
Original Assignee
Qiqihar University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiqihar University filed Critical Qiqihar University
Priority to CN202210170312.1A priority Critical patent/CN114381409B/en
Publication of CN114381409A publication Critical patent/CN114381409A/en
Application granted granted Critical
Publication of CN114381409B publication Critical patent/CN114381409B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A23K10/38—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Physiology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Botany (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Birds (AREA)
  • Sustainable Development (AREA)
  • Fodder In General (AREA)

Abstract

The invention belongs to the technical field of fermentation, and particularly relates to a fermented feed for improving animal production performance and/or improving feed utilization rate and application thereof. The fermented feed provided by the invention comprises a mixed microbial inoculum, wherein the mixed microbial inoculum comprises lactobacillus, saccharomyces cerevisiae and bacillus subtilis, and the ratio of viable bacteria to bacteria of the lactobacillus, the saccharomyces cerevisiae and the bacillus subtilis in the mixed microbial inoculum is (1-3): (1-3): (1-3); the effective viable count of the lactobacillus, the saccharomyces cerevisiae and the bacillus subtilis is (1-9) multiplied by 108CFU/g. The fermented feed improves the content of soluble protein in the corn protein powder through the mixed microbial inoculum, improves the digestible utilization rate of the corn protein powder, and improves the utilization rate of the corn protein powder.

Description

Fermented feed for improving animal production performance and/or improving feed utilization rate and application thereof
Technical Field
The invention belongs to the technical field of fermentation, and particularly relates to a fermented feed for improving animal production performance and/or improving feed utilization rate and application thereof.
Background
In recent years, the corn consumption of deep processing of corn in China is increased, about 300 million tons of corn protein powder which is a byproduct of corn starch processing is produced every year, and the yield of the byproduct is huge. The corn gluten meal is a high-protein feed, the protein content of the corn gluten meal is 55.0-60.0%, the corn gluten meal and the zein are mainly contained, wherein the glutamine content of the corn gluten meal is about one third of the protein content, the zein contains special amino acid composition, the corn gluten meal also contains high proportion of leucine and proline, and the special amino acid composition of the corn gluten meal enables the corn gluten meal to have the physiological functions of maintaining the intestinal function of animals, repairing stress, resisting oxidation, improving immunity and the like. Compared with common fish meal and bean cakes in feed industry, the corn protein powder can be used as feed, has obvious resource advantages, high feeding value and no toxic and harmful substances, does not need to be reprocessed, and can be directly used as a protein raw material.
However, the mode of directly mixing the corn protein powder into the feed is mostly adopted in the prior stage, and the main protein of the corn protein powder is alcohol soluble protein which has poor water solubility, can not be effectively absorbed and utilized by animals, has low digestibility and utilization rate and reduces the utilization rate of the corn protein powder.
Disclosure of Invention
The invention aims to overcome the problems in the prior art and provide a fermented feed for improving the production performance of animals and/or improving the utilization rate of the feed and application thereof.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention also provides a mixed microbial inoculum of the fermented feed, which comprises lactobacillus, saccharomyces cerevisiae and bacillus subtilis, wherein the ratio of viable bacteria to bacteria of the lactobacillus, the saccharomyces cerevisiae and the bacillus subtilis in the mixed microbial inoculum is (1-3): (1-3): (1-3); the effective viable count of the lactobacillus, the saccharomyces cerevisiae and the bacillus subtilis is respectively more than or equal to 1 multiplied by 10 in terms of per g or per mL of mixed microbial inoculum9CFU。
Preferably, the preservation number of the lactobacillus is CGMCCNo.13110;
the preservation number of the saccharomyces cerevisiae is CGMCC No. 21353;
the preservation number of the bacillus subtilis is CGMCC No. 21352.
The invention also provides a feed additive, which comprises the mixed microbial inoculum and a bottom material; the mass ratio of the mixed microbial inoculum to the bottom material is (2.5-12.5): 100.
preferably, the primer comprises a primer I or a primer II;
the first primer comprises the following components in parts by mass: 5 parts of corn protein powder, 3-4 parts of corn germ meal and 1-2 parts of guniting corn bran;
the second base material comprises the following components in parts by mass: 5-7 parts of corn protein powder, 1-3 parts of wheat bran, 1-2 parts of brewer's grain and 1 part of bone meal;
preferably, when the base material is a base material I, the feed additive further comprises sugar, and the mass ratio of the sugar to the base material is (2-15): 100.
the invention also provides a preparation method of the feed additive, which comprises the following steps:
mixing the bottom material and the mixed microbial inoculum with water, and carrying out anaerobic fermentation for 3-9 d to obtain a feed additive; the mass ratio of the base material to the water is 1g (0.8-1.6) mL.
Preferably, the temperature of the anaerobic fermentation is 25-35 ℃.
The invention also provides a fermented feed, which comprises the feed additive and the basic feed; the mass ratio of the feed additive to the basic feed is (10-15) in parts by mass of dry matter: (85-90).
The invention also provides the application of the mixed microbial inoculum or the feed additive prepared by the preparation method or the fermented feed in improving the production performance of animals, reducing the breeding cost and improving the utilization rate of the feed.
Preferably, the production properties include: dressing percentage, total thorax ratio, pectoral muscle ratio and abdominal fat ratio.
Has the advantages that:
the invention provides a mixed microbial inoculum for fermenting feed, which comprises lactobacillus, saccharomyces cerevisiae and bacillus subtilis, wherein the ratio of viable bacteria to bacteria of the lactobacillus, the saccharomyces cerevisiae and the bacillus subtilis in the mixed microbial inoculum is (1-3): (1-3): (1-3); the effective viable count of the lactobacillus, the saccharomyces cerevisiae and the bacillus subtilis is respectively more than or equal to 1 multiplied by 10 in terms of per g or per mL of mixed microbial inoculum9And (4) CFU. According to the invention, through the compound use of the lactobacillus, the saccharomyces cerevisiae and the bacillus subtilis in a certain proportion, the content of soluble protein in the corn protein powder is improved, the digestible utilization rate of the corn protein powder is improved, and the utilization rate of the corn protein powder is improved.
Furthermore, the feed additive provided by the invention improves the soluble protein content, small peptide content and hydrolase activity, especially protease and cellulase activity, in the feed additive through the mixed fermentation of specific mixed microbial inoculum and different bottom materials, so that the feed additive prepared by the invention has good nutritional safety and high digestibility and utilization rate.
Furthermore, the fermented feed provided by the invention can improve the production performance of animals, reduce the breeding cost and improve the utilization rate of the feed by adding the feed additive containing the mixed microbial inoculum; compared with unfermented feed, the feed has the advantages of high soluble protein content, high small peptide content, high hydrolase activity, good nutrition safety, high digestibility and high utilization rate, and can be used as good feed for feeding broiler chickens. The embodiment shows that the fermented feed prepared by the invention can be used for feeding 308 broiler chickens, so that the slaughtering performance of the broiler chickens can be obviously improved, and the digestibility of nutrient substances can be improved; the broiler chicken fed with the fermented feed prepared by the invention has the advantages that the body weight gain is obviously improved, the feed-weight ratio is obviously reduced, the cooking loss rate of leg muscles and breast muscles is obviously reduced, the meat quality of the broiler chicken is obviously improved, and the economic benefit is improved.
Biological preservation description:
lactobacillus YY-2, Latin is Lactobacillus sp, which is preserved in China general microbiological culture Collection center (CGMCC) at 2016, 10 and 13 days, with the preservation address of No. 3 Xilu-Beichen, No.1 Hakken, and the preservation number of CGMCC No. 13110.
Saccharomyces cerevisiae YY-4, named Saccharomyces cerevisiae, was deposited at China general microbiological culture Collection center (CGMCC) at 11.12.2020, with the collection address of No. 3, Siro No.1, Beijing, Chaoyang, and the collection number of CGMCC No. 21353.
Bacillus subtilis YY-3, Latin is Bacillus subtilis, which is preserved in China general microbiological culture Collection center (CGMCC) at 11 days 12 and 12 months 2020, the preservation address is No. 3 of Beijing Kogyang West Lu No.1 of the morning, and the preservation number is CGMCC No.21352 of the institute of microbiology of China academy of sciences.
Detailed Description
The invention provides a mixed microbial inoculum for fermenting feed, which comprises lactobacillus, saccharomyces cerevisiae and bacillus subtilis, wherein the ratio of viable bacteria to bacteria of the lactobacillus, the saccharomyces cerevisiae and the bacillus subtilis in the mixed microbial inoculum is (1-3): (1-3): (1-3), preferably (2-3): (1-2): (1-2), more preferably 3:2: 1; the effective viable count of the lactobacillus, the saccharomyces cerevisiae and the bacillus subtilis is respectively more than or equal to 1 multiplied by 10 in terms of per g or per mL of mixed microbial inoculum9CFU。
In the invention, the lactobacillus preferably comprises lactobacillus YY-2 with the preservation number of CGMCCNo.13110; the saccharomyces cerevisiae preferably comprises saccharomyces cerevisiae YY-4 with the preservation number of CGMCC No. 21353; the bacillus subtilis preferably comprises bacillus subtilis YY-3 with the preservation number of CGMCC No. 21352. The mixed microbial inoculum can specifically degrade the alcohol soluble protein with poor water solubility in the corn protein powder to form water soluble protein, so that the content of the soluble protein in the corn protein powder is improved, the digestibility and utilization rate of the corn protein powder are further improved, and the utilization rate of the corn protein powder is improved.
In the invention, the mixed bacterium agent is preferably a mixed bacterium liquid or a mixed bacterium powder, and more preferably a mixed bacterium liquid; the preparation method of the mixed microbial inoculum is not particularly limited, and the preparation method known by the technical personnel in the field can be adopted.
In the invention, the preparation of the mixed microbial inoculum is preferably to activate the preserved lactobacillus, saccharomyces cerevisiae and bacillus subtilis respectively, then inoculate the activated strains into corresponding liquid culture media respectively to culture to logarithmic phase, and mix the obtained seed solutions in proportion to obtain the mixed microbial inoculum.
In the invention, the activating culture medium of the bacillus subtilis is preferably an LB culture medium, specifically 10g of tryptone, 5g of yeast extract, 10g of sodium chloride, neutral pH and 15-20 g of agar, the volume is determined to be 1000mL, and the bacillus subtilis is sterilized by steam at 121 ℃ for 30 min.
In the invention, the activating culture medium of the saccharomyces cerevisiae is preferably a PDA culture medium, specifically 200g of potatoes and 20g of glucose, has natural pH, 15-20 g of agar, constant volume of 1000mL and is sterilized by steam at 121 ℃ for 30 min.
In the present invention, the activating medium of lactobacillus is preferably MRS medium: specifically, 10g of peptone, 10g of beef extract, 5g of yeast powder, 2g of each of dipotassium phosphate and diammonium citrate, 5g of sodium acetate, 20g of glucose, 801mL of tween, 0.58g of magnesium sulfate heptahydrate, 0.25g of manganese sulfate tetrahydrate and 15-20 g of agar are added, and the volume is adjusted to 1000 mL; adjusting the pH value to 6.2-6.6, and sterilizing for 30min by steam at 121 ℃.
In the present invention, the liquid culture media are preferably liquid culture media prepared according to the above-described formulation without agar. The three bacteria are preferably subjected to amplification culture in a shaking table, and the rotation speed of the shaking table is preferably 180-220 r/min; the temperature of the culture is preferably 30 ℃; preferably, the culture is terminated when the strain in the culture broth reaches the logarithmic growth phase.
According to the invention, through the compound use of the lactobacillus, the saccharomyces cerevisiae and the bacillus subtilis in a certain proportion, the alcohol soluble protein with poor water solubility in the corn protein powder can be degraded to form water soluble protein, so that the content of the soluble protein in the corn protein powder is improved, the digestibility and utilization rate of the corn protein powder is further improved, and the utilization rate of the corn protein powder is improved.
The invention also provides a feed additive, which comprises the mixed microbial inoculum, a bottom material and water in the scheme; the mass ratio of the mixed microbial inoculum to the bottom material is (2.5-12.5): 100.
in the present invention, the primer preferably includes primer one or primer two.
In the present invention, the first primer preferably comprises the following components in parts by mass: 5 parts of corn protein powder, 3-4 parts of corn germ meal and 1-2 parts of gunite corn bran, and further preferably comprises 5 parts of corn protein powder, 4 parts of corn germ meal and 1 part of gunite corn bran. In the invention, the mass ratio of the mixed microbial inoculum to the first base material is preferably (2.5-7.5): 100, more preferably 5: 100. In the present invention, the ratio of the mass of the first primer to the volume of water is preferably 1g (1.1 to 1.3) mL, and more preferably 1g:1.2 mL. In the invention, when the base material is a base material I, the feed additive preferably further comprises sugar, and the mass ratio of the sugar to the base material is preferably (2-15): 100, more preferably (5 to 10): 100, most preferably 5: 100; the sugar is preferably a crystallized glucose mother liquor. According to the invention, the crystal sugar mother liquor is added into the feed additive, so that a carbon source can be provided for the growth of the mixed microbial inoculum, and the production cost of the feed additive can be reduced.
In the invention, the second primer preferably comprises the following components in parts by mass: 5-7 parts of corn protein powder, 1-3 parts of wheat bran, 1-2 parts of brewer's grain and 1 part of bone meal, and further preferably comprises 5 parts of corn protein powder, 2 parts of wheat bran, 2 parts of brewer's grain and 1 part of bone meal. The bone powder is preferably purchased from Jipont bone powder produced by Jipont feed additive factory in the outer zone of the Harbin tract. In the invention, the mass ratio of the mixed microbial inoculum to the second base material is preferably (2.5-12.5): 100, more preferably (4.5-7.5): 100, and still more preferably 5: 100. In the present invention, the ratio of the mass of the second primer to the volume of water is preferably 1g (0.8 to 1.6) mL, more preferably 1g (1.1 to 1.3) mL, and still more preferably 1g:1.2 mL.
In the invention, the corn protein powder is mainly prolamin, and water-soluble protein can be formed by degrading the mixed microbial inoculum, so that the content of water-soluble protein in the feed additive is improved; the corn germ meal can provide albumin and albumin, and can provide energy substances for the mixed microbial inoculum; the guniting corn bran can be used as a loosening agent, is low in price, and can reduce the cost of the whole feed additive; the beer lees contains unknown growth factors and can be used as protein feed; the bone meal can be used as mineral feed for livestock to supplement calcium and phosphorus.
Unless otherwise specified, the present invention does not require any particular source for the components of the feed additive, and any commercially available product known to those skilled in the art may be used.
The invention also provides a preparation method of the feed additive, which comprises the following steps:
and mixing the bottom material and the mixed bacteria with water, and carrying out anaerobic fermentation for 3.5-9 days to obtain the feed additive.
The bottom material, the mixed bacteria and water are mixed, and anaerobic fermentation is carried out for 3.5-9 days, so as to obtain the feed additive. In the present invention, when the base material is a base material, the preparation of the fermentation material preferably further comprises adding a mother liquor of crystallized glucose. The present invention does not require any special mixing means, and can be carried out by mixing means known to those skilled in the art.
In the invention, the time for anaerobic fermentation is 3.5-9 days, more preferably 3.5-7 days, and most preferably 3.5-5 days; the temperature of the anaerobic fermentation is preferably 25-35 ℃, and more preferably 30-32 ℃; the anaerobic fermentation container preferably comprises a one-way valve fermentation bag; the mode of fermentation preferably comprises raw meal fermentation.
In the invention, the fermentation time is preferably 3-5 d after bag expansion, and is more preferably 3 d; the starting time of the bag expansion is determined according to different seasons, wherein the spring and summer season is 12 hours after the bagging starts, and the autumn and winter season is 48 hours after the bagging starts.
In the invention, the method for the one-way valve bagged fermentation preferably comprises a bag turning operation after 2d of fermentation. According to the invention, the caking of the bottom materials can be avoided by turning the bag, and meanwhile, the strains and the bottom materials can be better mixed, so that the fermentation is promoted.
According to the invention, the feed additive with high soluble protein content and multiple active ingredients is prepared by compounding the lactobacillus, the saccharomyces cerevisiae and the bacillus subtilis in a certain proportion and controlling the components and the proportion of a fermentation substrate, the material-water ratio, the inoculation amount, the fermentation time and the fermentation temperature, and the soluble protein content, the small peptide content and the hydrolase activity, especially the protease and cellulase activity, are improved compared with the unfermented substrate, so that the feed additive prepared by the invention has good nutritional safety and high digestibility and utilization rate.
The invention also provides a fermented feed, which comprises the feed additive and the basic feed in the scheme, wherein the mass ratio of the feed additive to the basic feed is (10-15) in parts by mass of dry matters: (85-90); more preferably 15: 85.
In the present invention, the base feed preferably comprises the following components in parts by mass of dry matter: 584-620 parts of corn, 86-160 parts of soybean meal, 60-100 parts of corn protein powder, 15 parts of calcium hydrophosphate, 10 parts of stone powder, 67 parts of wheat bran, 15 parts of soybean oil, 3 parts of salt and 10 parts of premix; more preferably: 584-590 parts of corn, 86-130 parts of soybean meal, 60 parts of corn protein powder, 15 parts of calcium hydrophosphate, 10 parts of stone powder, 67 parts of wheat bran, 15 parts of soybean oil, 3 parts of salt and 10 parts of premix.
In the invention, the fermented feed preferably further comprises exogenous lysine, and the mass ratio of the exogenous lysine to the total mass of the basic feed and the feed additive is (0.3-1.2): 1000.
unless otherwise specified, the present invention does not require any particular source for the components of the fermented feed, and any commercially available product known to those skilled in the art may be used.
According to the fermented feed provided by the invention, the feed additive containing the mixed microbial inoculum is added, so that the production performance of animals can be improved, the breeding cost is reduced, and the utilization rate of the feed is improved. The embodiment shows that the fermented feed prepared by the invention can be used for feeding 308 broiler chickens, so that the slaughtering performance of the broiler chickens can be obviously improved, and the digestibility of nutrient substances can be improved; the broiler chicken fed with the fermented feed prepared by the invention has the advantages that the body weight gain is obviously improved, the feed-weight ratio is obviously reduced, the cooking loss rate of leg muscles and breast muscles is obviously reduced, the meat quality of the broiler chicken is obviously improved, and the economic benefit is improved.
The invention also provides the application of the feed additive or the fermented feed in the scheme in improving the production performance of animals, reducing the breeding cost and improving the utilization rate of the feed; the animals preferably include livestock and poultry animals; the livestock and poultry preferably comprise broiler chickens; the broiler preferably comprises broiler chicken.
In order to further illustrate the present invention, the mixed microbial inoculum including fermented feed, the feed additive and the preparation method thereof, the fermented feed and the application thereof provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
A mixed microbial inoculum for fermented feed is prepared from the following raw materials in parts by mass: 3 parts of lactobacillus (with the preservation number of CGMCCNo.13110), 2 parts of saccharomyces cerevisiae (with the preservation number of CGMCCNo.21353) and 1 part of bacillus subtilis (with the preservation number of CGMCCNo.21352).
The preparation of the mixed microbial inoculum comprises the following steps:
respectively activating lactobacillus, saccharomyces cerevisiae and bacillus subtilis, and then respectively inoculating the activated strains into corresponding liquid culture media for shake cultivation (200r/min), wherein the cultivation dimension is 30 ℃; culturing to logarithmic growth phase to obtain seed liquid (effective viable count of three bacteria in different seed liquid is 1 × 10 respectively)9CFU/mL); and finally, mixing according to the volume ratio to obtain the mixed microbial inoculum.
The activation culture medium of the bacillus subtilis is an LB culture medium, specifically 10g of tryptone, 5g of yeast extract, 10g of sodium chloride, neutral pH and 20g of agar, the volume is determined to be 1000mL, and steam sterilization is carried out at 121 ℃ for 30 min;
the activating culture medium of the saccharomyces cerevisiae is a PDA culture medium, specifically 200g of potatoes and 20g of glucose, the pH is natural, 20g of agar is added, the volume is determined to be 1000mL, and steam sterilization is carried out at 121 ℃ for 30 min;
the activating culture medium of the lactobacillus is MRS culture medium: specifically, 10g of peptone, 10g of beef extract, 5g of yeast powder, 2g of each of dipotassium phosphate and diammonium citrate, 5g of sodium acetate, 20g of glucose, 801mL of tween, 0.58g of magnesium sulfate heptahydrate, 0.25g of manganese sulfate tetrahydrate and 20g of agar are added to the volume of 1000 mL. Adjusting pH to 6.4, and steam sterilizing at 121 deg.C for 30 min;
example 2
A feed additive is prepared from the following raw materials in parts by mass: 5 parts of corn protein powder, 4 parts of corn germ meal, 1 part of gunite corn bran, 0.5 part of mixed microbial inoculum prepared in example 1 and 0.5 part of crystallized glucose mother liquor.
The preparation of the feed additive comprises the following steps:
5kg of corn protein powder, 4kg of corn germ meal, 1kg of gunite corn bran, 0.5kg of mixed microbial inoculum prepared in example 1, 0.5kg of crystalline glucose mother liquor and 12L of water are mixed, stirred uniformly, put into a one-way valve fermentation bag, sealed, fermented at 30 ℃, and subjected to primary bag turning operation on the 2 nd day of fermentation. And (5) fermenting for 3 days after bag expansion to obtain the feed additive.
Example 3
A feed additive is prepared from the following raw materials in parts by mass: 5 parts of corn protein powder, 2 parts of wheat bran, 2 parts of brewer's grains, 1 part of bone meal and 0.5 part of the mixed microbial inoculum prepared in the example 1.
The preparation of the feed additive comprises the following steps:
5kg of corn protein powder, 2kg of wheat bran, 2kg of brewer's grains, 1kg of bone meal, 0.5kg of the mixed microbial inoculum prepared in example 1 and 12L of water are mixed, stirred uniformly, put into a one-way valve fermentation bag, sealed, fermented at 30 ℃, and subjected to primary bag turning operation on the 2 nd day of fermentation. And (5) fermenting for 3 days after bag expansion to obtain the feed additive.
Example 4
A feed additive similar to that of example 2, except that the amount of water used was 8L.
Example 5
A feed additive similar to that of example 3, except that the amount of water used was 16L.
Example 6
A feed additive is prepared from the following raw materials in parts by mass: 5 parts of corn protein powder, 2 parts of wheat bran, 2 parts of brewer's grains, 1 part of bone meal and 0.25 part of the mixed microbial inoculum prepared in the example 1.
The preparation of the feed additive comprises the following steps:
5kg of corn protein powder, 2kg of wheat bran, 2kg of brewer's grains, 1kg of bone meal, 0.25kg of the mixed microbial inoculum prepared in example 1 and 12L of water are mixed, stirred uniformly, put into a one-way valve fermentation bag, sealed, fermented at 30 ℃, and subjected to primary bag turning operation on the 2 nd day of fermentation. And (5) fermenting for 3 days after bag expansion to obtain the feed additive.
Example 7
A feed additive is prepared from the following raw materials in parts by mass: 5 parts of corn protein powder, 2 parts of wheat bran, 2 parts of brewer's grains, 1 part of bone meal and 1.25 parts of the mixed microbial inoculum prepared in the example 1.
The preparation of the feed additive comprises the following steps:
5kg of corn protein powder, 2kg of wheat bran, 2kg of brewer's grains, 1kg of bone meal, 1.25kg of the mixed microbial inoculum prepared in the example 1 and 12L of water are mixed, stirred uniformly, put into a one-way valve fermentation bag, sealed, fermented at 30 ℃, and subjected to primary bag turning operation on the 2 nd day of fermentation. And (5) fermenting for 3 days after bag expansion to obtain the feed additive.
Example 8
A feed additive is prepared from the following raw materials in parts by mass: 5 parts of corn protein powder, 4 parts of corn germ meal, 1 part of gunite corn bran, 0.5 part of mixed microbial inoculum prepared in example 1 and 0.2 part of crystallized glucose mother liquor.
The preparation of the feed additive comprises the following steps:
5kg of corn protein powder, 4kg of corn germ meal, 1kg of gunite corn bran, 0.5kg of mixed microbial inoculum prepared in example 1, 0.2kg of crystalline glucose mother liquor and 12L of water are mixed, stirred uniformly, put into a one-way valve fermentation bag, sealed, fermented at 30 ℃, and subjected to primary bag turning operation on the 2 nd day of fermentation. And (5) fermenting for 3 days after bag expansion to obtain the feed additive.
Example 9
A feed additive is prepared from the following raw materials in parts by mass: 5 parts of corn protein powder, 4 parts of corn germ meal, 1 part of gunite corn bran, 0.5 part of mixed microbial inoculum prepared in example 1 and 1.5 parts of crystallized glucose mother liquor.
The preparation of the feed additive comprises the following steps:
5kg of corn protein powder, 4kg of corn germ meal, 1kg of gunite corn bran, 0.5kg of mixed microbial inoculum prepared in example 1, 1.5kg of crystalline glucose mother liquor and 12L of water are mixed, stirred uniformly, put into a one-way valve fermentation bag, sealed, fermented at 30 ℃, and subjected to primary bag turning operation on the 2 nd day of fermentation. And (5) fermenting for 3 days after bag expansion to obtain the feed additive.
Example 10
A fermented feed is prepared from the following dry materials in parts by mass: 100 parts of feed additive prepared in example 2, 590 parts of corn, 130 parts of soybean meal, 60 parts of corn protein powder, 15 parts of calcium hydrophosphate, 10 parts of stone powder, 67 parts of wheat bran, 15 parts of soybean oil, 3 parts of salt premix 10 parts and 0.3 part of exogenous lysine.
The preparation method of the fermented feed comprises the following steps:
100g (dry matter mass) of the feed additive prepared in example 2, 590g (dry matter mass) of corn, 130g (dry matter mass) of soybean meal, 60g (dry matter mass) of corn gluten meal, 15g of calcium hydrogen phosphate, 10g of stone powder, 67g (dry matter mass) of wheat bran, 15g of soybean oil, 3g of salt, 10g (dry matter mass) of premix, and 0.3g of exogenous lysine were mixed to obtain fermented feed.
Example 11
A fermented feed is prepared from the following dry materials in parts by mass: 150 parts of feed additive prepared in example 2, 584 parts of corn, 86 parts of soybean meal, 60 parts of corn protein powder, 15 parts of calcium hydrophosphate, 10 parts of stone powder, 67 parts of wheat bran, 15 parts of soybean oil, 3 parts of salt, 10 parts of premix and 1.2 parts of exogenous lysine.
The preparation method of the fermented feed comprises the following steps:
150g (dry matter mass) of the feed additive prepared in example 2, 584g (dry matter mass) of corn, 86g (dry matter mass) of soybean meal, 60g (dry matter mass) of corn gluten meal, 15g of calcium hydrogen phosphate, 10g of stone powder, 67g (dry matter mass) of wheat bran, 15g of soybean oil, 3g of salt, 10g (dry matter mass) of premix, and 1.2g of exogenous lysine were mixed to obtain a fermented feed.
Comparative example 1
A basic feed is prepared from the following dry substances in parts by mass: 620 parts of corn, 160 parts of soybean meal, 100 parts of corn protein powder, 15 parts of calcium hydrophosphate, 10 parts of stone powder, 67 parts of wheat bran, 15 parts of soybean oil, 3 parts of salt and 10 parts of premix.
The basic feed is prepared by the following steps:
620g of corn (dry matter mass), 160g of soybean meal (dry matter mass), 100g of corn protein powder (dry matter mass), 15g of calcium hydrophosphate, 10g of stone powder, 67g of wheat bran (dry matter mass), 15g of soybean oil, 3g of salt and 10g of premix (dry matter mass) are mixed to obtain fermented feed, and the basic feed is obtained.
Application example 1
The nutrient content and active ingredients of the bottom material before and after fermentation in example 2 were measured, and the fermented product was subjected to composition analysis.
Measurement items and methods: the crude protein content, the crude fat content, the crude fiber content, the crude ash content, the calcium content and the phosphorus content refer to GB/T6432-. The soluble protein content is measured by adopting a Folin-phenol method. The protease activity is determined according to GB/T23527-2009, the lipase activity is determined according to an olive oil emulsification method, the cellulase activity is determined according to a DNS method, the small peptide content is determined according to a trichloroacetic acid (TCA) precipitation method, and the viable count is determined by a coating flat plate counting method. The results of measuring the basic nutrient content of the basic material before and after fermentation are shown in Table 1, and the results of measuring the active ingredients of the basic material before and after fermentation are shown in Table 2.
TABLE 1 measurement results of basic nutrient contents of the bottom materials before and after fermentation
Composition (I) Before fermentation (%) After fermentation (%)
Crude protein content 38.65±0.04 40.23±0.23
Crude fat content 6.82±0.17 7.94±0.52
Crude fiber content 9.52±0.95 6.65±0.58
Calcium content 0.21±0.04 0.23±0.02
Phosphorus content 0.52±0.01 0.53±0.12
Ash content 3.65±0.56 3.67±0.82
Soluble proteins 2.98±0.24 9.37±0.69
As can be seen from Table 1, the feed additive prepared by the invention has the advantages that the crude fat content and the soluble protein content of the unfermented bottom material are respectively increased by 1.12 percent and 6.39 percent, and the crude fiber content is reduced by 2.87 percent, so that the feed additive prepared by the invention has good nutritional safety, can be digested and utilized, has the reduced fiber content, is beneficial to the digestion of animal intestinal tracts and is more beneficial to the digestion and absorption of broilers.
TABLE 2 results of active ingredient determination of the bottom materials before and after fermentation
Composition (I) Before fermentation (%) After fermentation (%)
Protease activity U/g 157.54±23.54 953.75±59.65
Cellulase activity U/g 64.53±32.31 435.24±52.65
Lipase activity U/g 5.36±0.95 25.63±0.36
Content of small peptide% 0.95±0.21 3.54±0.15
Total number of beneficial bacteria cfu/g / 1.55*108
As can be seen from Table 2, after microbial fermentation, the activity of three hydrolases in the feed is obviously improved, and the content of small peptide is improved by 2.59% compared with that before fermentation.
Compared with the unfermented bottom material, the feed additive prepared by the invention improves the total number of beneficial bacteria, the content of small peptides and the activity of hydrolase, especially the activity of protease and cellulase, and meanwhile, the active small peptides generated by degrading crude protein by microbial protease can obviously improve the intestinal health of animals and improve the immunity of organisms. The feed additive prepared by the invention has good nutritional safety and high digestibility and utilization rate.
Application example 2
The nutrient content and active ingredients of the bottom material before and after fermentation in example 3 were measured by the same measuring method as in application example 1, and the fermented product was subjected to component analysis.
The results of the measurement of the active ingredients of the bottom materials before and after fermentation are shown in Table 3.
TABLE 3 measurement results of basic nutrient contents of the bottom materials before and after fermentation
Composition (I) Before fermentation (%) After fermentation (%)
Crude protein content 32.52±0.05 33.41±0.13
Crude fat content 8.54±0.24 9.87±0.41
Crude fiber content 10.65±0.24 5.84±0.35
Calcium content 3.57±0.08 3.65±0.04
Phosphorus content 1.35±0.02 1.37±0.11
Ash content 6.79±0.71 6.72±0.64
Soluble proteins 1.97±0.32 8.83±0.46
As can be seen from Table 3, the feed additive prepared in example 3 of the present invention has increased soluble protein content relative to the unfermented substrate, and the crude fiber content is significantly reduced, such that the feed additive prepared in the present invention has good nutritional safety, high digestibility, and is more conducive to digestion and absorption by broilers.
Application example 3
90 broilers of 1 day age, 308, are selected, are subjected to normal professional brooding daily ration feeding for 7 days, and then are subjected to pre-feeding on the basic feed prepared in the comparative example 1 for 7 days, after the pre-feeding period is finished, the broilers are averagely divided into 3 groups according to male and female weights from 14 days of age, the 3 groups are respectively a fermentation group for feeding the fermented feed prepared in the examples 10 and 11 and a control group for the basic feed prepared in the comparative example 1, each group is 3 times repeated, 10 broilers are repeated (adjustable according to growth conditions), are subjected to formal feeding, and are fed to 42 days of age. The test date is 14d pre-feeding period, and 28d test feeding period. The chicken house is normally lighted every day, and the chicken house is freely fed with drinking water, fed and cleaned. The basic ration (comparative example 1) was formulated according to the nutritional requirements of NRC (1994) and broiler feeding Standard (NY/T33-2004) in our country, and the ingredients of the fermented feeds prepared in examples 10 and 11 and the basic feed prepared in comparative example 1 are shown in Table 4.
TABLE 4 ingredients of different feeds
Figure BDA0003517830580000131
Figure BDA0003517830580000141
Note: since the fermented feeds of examples 10 and 11 had a soybean meal content lower than that of the basal feed prepared in comparative example 1, exogenous lysine was added during the preparation of the fermented feeds so that the contents of lysine in the final three feeds were substantially the same.
Measurement of growth Performance
After formal feeding, the test broilers are fasted for 12 hours every 7 days from the age of 14 days, and then the weight and the feed consumption of the test broilers are weighed. And (3) recording experimental data, calculating the body weight gain, the material consumption weight and the material weight ratio, and obtaining a calculation result shown in a table 5.
TABLE 5 growth Performance of broiler chickens
Figure BDA0003517830580000142
Figure BDA0003517830580000151
Note: the data in the table are mean values, and different lower case letters indicate that the difference between samples is statistically significant (P < 0.05).
As can be seen from Table 5, the "308" broiler weight gain of the fermented feed prepared in example 10 and the fermented feed prepared in example 11 was significantly higher than that of the control group (P <0.05) from the time of feeding to 28 days of age. In combination with the feed weight ratios at each day's age, at 28 days of age, both fermentation groups were significantly lower than the control 1 feed group (P <0.05), but there was no significant difference between the two groups of examples 10 and 11 (P > 0.05). The feed-to-weight ratio at 35 days of age was also significantly lower in the fermentation group than in the control group (P <0.05), and significantly lower in the example 11 group than in the example 10 group (P < 0.05). At 42 days of age, the feed-to-weight ratio of the example 10 group was significantly lower than that of the control group (P <0.05), and the example 11 group was not significantly different although lower than that of the control group (P > 0.05).
In conclusion, the feed additive added into the daily ration can obviously improve the growth performance of the broiler chicken, but the advantage of adding the fermented feed into the daily ration in the later growth period of the broiler chicken is more obvious compared with the common daily ration, and the fermented feed prepared in the embodiment 11 is more suitable for the animal organism.
Slaughter performance determination
At the end of the experiment, one of the animals was randomly slaughtered in each group of animals in parallel for 6 times, weighed, bled from the neck, scalded in a water bath at 65 ℃ for 1min, and depilated, and the weight of the carcass, the weight of the complete chest, the weight of the bilateral pectoral muscles, the weight of the bilateral leg muscles, the weight of the abdominal fat and the weight of the fat around the muscular stomach were weighed. And (4) calculating slaughter performance indexes. The calculation formula is as follows:
slaughter rate (%) (carcass weight/live weight) × 100
Total bore ratio (%) (total bore weight/live weight) × 100
Breast muscle ratio (%) (bilateral breast muscle weight/total dry weight) × 100
Leg muscle ratio (%) (leg muscle weight on both sides/total bore weight) × 100
Abdominal fat percentage (%) [ (abdominal fat weight + fat weight around muscular stomach)/total dry weight ] × 100.
The slaughter performance of the 308 broiler chickens was analyzed, and the analysis results are shown in Table 6.
TABLE 6 slaughter Performance (%)
Figure BDA0003517830580000152
Figure BDA0003517830580000161
Note: the data in the table are mean values and the row data are shouldered with different lower case letters to indicate significant differences (P < 0.05).
As can be seen from Table 6, the dressing percentage, the total bore ratio, the pectoral muscle ratio and the abdominal fat ratio of the group of example 11 were significantly higher than those of the control group (P <0.05), the pectoral muscle ratio of the group of example 10 was significantly higher than that of the control group (P <0.05), and the remaining indexes were not significantly improved relative to the control group (P > 0.05). It is shown that the broiler chickens of the group of example 11 had a sufficient amount of meat and had high economic efficiency and less waste than those of the control group and the group of example 10. The result shows that the slaughtering performance of the broiler chickens can be obviously improved by adding the fermented feed into the daily ration, but the obvious effect can be achieved only when the addition amount reaches 15%.
Viscera index of broiler chicken
At 42 days of age after the end of the trial, 1 chicken from each replicate was slaughtered in parallel 6 times. Weighing, bleeding, scalding in water bath at 65 deg.C for 1min, removing hair, weighing carcass weight, bursa weight, liver weight, spleen weight, heart weight, muscular stomach weight and glandular stomach weight, and calculating the results shown in Table 8. The index of each organ is calculated according to the following formula:
bursal index (%) (bursal weight/carcass weight) × 100
Liver index (%) (liver weight/carcass weight) × 100
Spleen index (%) (spleen weight/carcass weight) × 100
Heart index (%) (heart weight/carcass weight) × 100
Myogastric index (%) - (myogastric weight/carcass weight) × 100
Glandular stomach index (%) (glandular stomach weight/carcass weight) × 100
TABLE 7 viscera index of broiler chickens
Figure BDA0003517830580000162
Figure BDA0003517830580000171
As is clear from Table 7, the organ indexes of the 10% fermentation group and the 15% fermentation group were higher than those of the control group, but were not significantly different from those of the control group (P > 0.05). The fact that the fermented feed added into the daily ration can improve the development degree of immune organs of the broiler chicken, but the development degree is not obviously improved.
Meat quality analysis
At the end of the test, 1 chicken from each replicate was slaughtered at 42 days of age, in parallel 6 times, and the pectoral and leg muscles were separated, packed tightly with valve bags and stored at 4 ℃ for meat quality determination.
Muscle pH: the pH of the pectoral and leg muscles was measured within 45min after slaughter (pH45min), and after 24h storage at 4 ℃ the pH of the pectoral and leg muscles was measured (pH24 h). Each sample was measured 3 times and the average was taken.
Muscle drip loss rate: taking about 10g of pectoral and leg muscles stored for 24h at 4 ℃, weighing W1, hooking one end of a meat sample with a thin iron wire to make muscle fibers vertically hung downwards into a disposable paper cup, then putting into a self-sealing bag, fastening the mouth of the bag, putting into a refrigerator at 4 ℃, taking out after 24h, and weighing W2 again.
Muscle cooking loss rate: the pectoral and leg muscles after 24h storage at 4 ℃ were taken. Cutting the meat sample into 3.5cm × 3.5cm × 5cm cuboid blocks, weighing W1, sealing with a valve bag, placing in 80 deg.C constant temperature water bath for 10min, cooling at room temperature when the central temperature reaches 70 deg.C, and weighing W2.
Muscle shear force: the meat sample after being analyzed for cooking loss and cooled to room temperature is taken for shear force measurement. Each meat sample was measured in triplicate and the average was taken for the measurements shown in Table 8.
TABLE 8 influence of microbial fermented feed for broiler chickens on meat quality of '308' broiler chickens
Figure BDA0003517830580000172
Figure BDA0003517830580000181
Note: the data in the table are mean values and the row data are shouldered with different lower case letters to indicate significant differences (P < 0.05).
As can be seen from Table 8, the pH values of the two fermentation groups were not significantly changed from the control group (P > 0.05), the boiling loss rate of the leg muscles and the chest muscles of the 10% fermentation group was significantly lower than that of the control group (P <0.05), the drip loss rate of the leg muscles and the chest muscles of the 15% fermentation group was significantly lower than that of the control group (P <0.05), and the muscle shear force indicated the hardness of the meat, so that the meat of the broiler chicken of the 10% fermentation group was tender. In conclusion, the meat quality of the broiler chicken can be obviously improved by adding the fermented feed into the daily ration, and the economic benefit is improved, but compared with the meat quality of a 10% fermented group, the meat quality of a 15% fermented group is better.
In conclusion, the fermented feed provided by the invention can improve the production performance of animals, reduce the breeding cost and improve the utilization rate of the feed by adding the feed additive, and the slaughtering performance of broiler chickens can be obviously improved and the digestibility of nutrient substances can be improved by using the fermented feed prepared by the invention in the feeding of 308 broiler chickens; the broiler chicken fed with the fermented feed prepared by the invention has the advantages that the body weight gain is obviously improved, the feed-weight ratio is obviously reduced, the cooking loss rate of leg muscles and breast muscles is obviously reduced, the meat quality of the broiler chicken is obviously improved, and the economic benefit is improved.
Although the present invention has been described in detail with reference to the above embodiments, it is only a part of the embodiments of the present invention, not all of the embodiments, and other embodiments can be obtained without inventive step according to the embodiments, and the embodiments are within the scope of the present invention.

Claims (10)

1. The mixed microbial inoculum for fermenting the feed is characterized by comprising lactobacillus, saccharomyces cerevisiae and bacillus subtilis, wherein the ratio of viable bacteria to bacteria of the lactobacillus, the saccharomyces cerevisiae and the bacillus subtilis in the mixed microbial inoculum is (1-3): (1-3): (1-3); the effective viable count of the lactobacillus, the saccharomyces cerevisiae and the bacillus subtilis is respectively more than or equal to 1 multiplied by 10 in terms of per g or per mL of mixed microbial inoculum9CFU。
2. The mixed bacterial agent as claimed in claim 1, wherein the lactobacillus has a preservation number of CGMCCNo.13110;
the preservation number of the saccharomyces cerevisiae is CGMCC No. 21353;
the preservation number of the bacillus subtilis is CGMCC No. 21352.
3. A feed additive, which comprises the mixed microbial inoculum of claim 1 or 2 and a substrate; the mass ratio of the mixed microbial inoculum to the bottom material is (2.5-12.5): 100.
4. the feed additive of claim 3 wherein the substrate comprises substrate one or substrate two;
the first primer comprises the following components in parts by mass: 5 parts of corn protein powder, 3-4 parts of corn germ meal and 1-2 parts of guniting corn bran;
the second base material comprises the following components in parts by mass: 5-7 parts of corn protein powder, 1-3 parts of wheat bran, 1-2 parts of brewer's grain and 1 part of bone meal.
5. The feed additive as claimed in claim 4, wherein when the base material is base material one, the feed additive further comprises sugar, and the mass ratio of the sugar to the base material is (2-15): 100.
6. a process for the preparation of a feed additive according to any one of claims 3 to 5, comprising the steps of:
mixing the bottom material and the mixed microbial inoculum with water, and carrying out anaerobic fermentation for 3-9 d to obtain a feed additive; the mass ratio of the base material to the water is 1g (0.8-1.6) mL.
7. The method according to claim 6, wherein the temperature of the anaerobic fermentation is 25 to 35 ℃.
8. A fermented feed, characterized in that the fermented feed comprises the feed additive according to any one of claims 2 to 5 and a basal feed; the mass ratio of the feed additive to the basic feed is (10-15) in parts by mass of dry matter: (85-90).
9. Use of the mixed bacterial agent of claim 1 or 2, the feed additive of any one of claims 3 to 5, the feed additive prepared by the preparation method of claim 6 or 7, or the fermented feed of claim 8 for improving animal production performance and/or improving feed utilization rate.
10. Use according to claim 9, wherein said production properties comprise: one or more of dressing percentage, total bore ratio, pectoral muscle ratio, and abdominal fat ratio.
CN202210170312.1A 2022-02-24 2022-02-24 Fermented feed for improving animal productivity and/or improving feed utilization rate and application thereof Active CN114381409B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210170312.1A CN114381409B (en) 2022-02-24 2022-02-24 Fermented feed for improving animal productivity and/or improving feed utilization rate and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210170312.1A CN114381409B (en) 2022-02-24 2022-02-24 Fermented feed for improving animal productivity and/or improving feed utilization rate and application thereof

Publications (2)

Publication Number Publication Date
CN114381409A true CN114381409A (en) 2022-04-22
CN114381409B CN114381409B (en) 2023-08-18

Family

ID=81206008

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210170312.1A Active CN114381409B (en) 2022-02-24 2022-02-24 Fermented feed for improving animal productivity and/or improving feed utilization rate and application thereof

Country Status (1)

Country Link
CN (1) CN114381409B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114891680A (en) * 2022-05-20 2022-08-12 中国农业科学院饲料研究所 Predigested feed for improving health and growth of herbivorous and omnivorous aquatic animals

Citations (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100080266A (en) * 2008-12-29 2010-07-08 주식회사 대호 Probiotics for feed additives
CN105029080A (en) * 2013-08-20 2015-11-11 沈阳科丰牧业科技有限公司 Method for improving production performance and chicken quality of broiler chicken by means of fungicide
CN105543131A (en) * 2015-12-30 2016-05-04 中粮饲料有限公司 Compound bacteria/cottonseed meal fermented feed and preparation method thereof
CN105661011A (en) * 2016-01-22 2016-06-15 重庆民泰香料化工有限责任公司 Functional bio-protein feed ferment and fermented protein feed
CN106942470A (en) * 2017-03-22 2017-07-14 南京致润生物科技有限公司 Biological fermentation feed produced using agricultural wastes and its preparation method and application
CN107006677A (en) * 2017-04-27 2017-08-04 齐齐哈尔大学 A kind of feed and its application rich in probiotics and active peptide
CN107006678A (en) * 2017-04-27 2017-08-04 齐齐哈尔大学 Corn protein powder feature fermented feed and its application
CN108034623A (en) * 2018-01-29 2018-05-15 天津博菲德科技有限公司 A kind of compound microbial culture starter and its application
CN108441452A (en) * 2018-04-13 2018-08-24 天津博菲德科技有限公司 A kind of store pig compound microbial culture starter and its application
CN111227111A (en) * 2020-01-14 2020-06-05 齐齐哈尔大学 Microbial fermentation feed and application
CN111513177A (en) * 2020-04-09 2020-08-11 宁夏乙征生物工程有限公司 Preparation method of Chinese herbal medicine fermented feed
CN111820332A (en) * 2020-07-03 2020-10-27 陕西石羊农业科技股份有限公司 Chinese herbal medicine laying hen feed and preparation method thereof
CN112806497A (en) * 2021-01-21 2021-05-18 乐山市农业科学研究院 Broiler tea residue bacterium enzyme synergistic fermented feed and preparation method thereof
CN116144555A (en) * 2023-03-22 2023-05-23 齐齐哈尔大学 Fermented feed additive for antagonizing intestinal damage of broiler chickens and preparation method and application thereof

Patent Citations (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100080266A (en) * 2008-12-29 2010-07-08 주식회사 대호 Probiotics for feed additives
CN105029080A (en) * 2013-08-20 2015-11-11 沈阳科丰牧业科技有限公司 Method for improving production performance and chicken quality of broiler chicken by means of fungicide
CN105543131A (en) * 2015-12-30 2016-05-04 中粮饲料有限公司 Compound bacteria/cottonseed meal fermented feed and preparation method thereof
CN105661011A (en) * 2016-01-22 2016-06-15 重庆民泰香料化工有限责任公司 Functional bio-protein feed ferment and fermented protein feed
CN106942470A (en) * 2017-03-22 2017-07-14 南京致润生物科技有限公司 Biological fermentation feed produced using agricultural wastes and its preparation method and application
CN107006677A (en) * 2017-04-27 2017-08-04 齐齐哈尔大学 A kind of feed and its application rich in probiotics and active peptide
CN107006678A (en) * 2017-04-27 2017-08-04 齐齐哈尔大学 Corn protein powder feature fermented feed and its application
CN108034623A (en) * 2018-01-29 2018-05-15 天津博菲德科技有限公司 A kind of compound microbial culture starter and its application
CN108441452A (en) * 2018-04-13 2018-08-24 天津博菲德科技有限公司 A kind of store pig compound microbial culture starter and its application
CN111227111A (en) * 2020-01-14 2020-06-05 齐齐哈尔大学 Microbial fermentation feed and application
CN111513177A (en) * 2020-04-09 2020-08-11 宁夏乙征生物工程有限公司 Preparation method of Chinese herbal medicine fermented feed
CN111820332A (en) * 2020-07-03 2020-10-27 陕西石羊农业科技股份有限公司 Chinese herbal medicine laying hen feed and preparation method thereof
CN112806497A (en) * 2021-01-21 2021-05-18 乐山市农业科学研究院 Broiler tea residue bacterium enzyme synergistic fermented feed and preparation method thereof
CN116144555A (en) * 2023-03-22 2023-05-23 齐齐哈尔大学 Fermented feed additive for antagonizing intestinal damage of broiler chickens and preparation method and application thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114891680A (en) * 2022-05-20 2022-08-12 中国农业科学院饲料研究所 Predigested feed for improving health and growth of herbivorous and omnivorous aquatic animals
CN114891680B (en) * 2022-05-20 2023-09-15 中国农业科学院饲料研究所 Pre-digestion feed for improving health and growth of herbivorous and omnivorous aquatic animals

Also Published As

Publication number Publication date
CN114381409B (en) 2023-08-18

Similar Documents

Publication Publication Date Title
KR100815851B1 (en) Fermented Mulberry Leaf Feed Additives Using Mulberry Leaves and Useful Microorganisms, Methods for Manufacturing the Duck Meat and Chicken Meat
CN102168045B (en) Bacillus subtilis preparation and preparation method thereof
CN105595008B (en) Laying period compound feed for improving healthy chick rate of egg-laying hens and preparation method thereof
CN101940267B (en) Method for preparing microbial fermentation feed for improving pork quality
CN110679728A (en) Preparation method and application of fermented rice bran feed
CN106974063B (en) Method for producing high-efficiency protein feed by using feed enzyme in cooperation with bacillus coagulans
CN102726596B (en) Feather protein peptide feed additive and application thereof
CN103262963B (en) Live pig fattening feed taking fermented agricultural and sideline products as raw materials
CN111436526A (en) Preparation method and application of fermented rice bran meal with bacterium enzyme for improving growth performance of fattening pigs
CN104106726A (en) New probiotic, and probiotic fermentation feed prepared by using it
WO2023159960A1 (en) Probiotic lactobacillus plantarum and application thereof in preparation of low-salt fermented meat food product
CN104012758A (en) Biological fermentation feed and preparation method thereof
CN112189770A (en) Antibiotic-free grass carp feed containing fermented mulberry leaves and preparation method thereof
CN107312732A (en) A kind of probiotic feed additive
CN109287883B (en) Compound fermented feed additive for egg-laying poultry, preparation method and application thereof
CN114381409B (en) Fermented feed for improving animal productivity and/or improving feed utilization rate and application thereof
CN110301526A (en) Complex micro organism fungicide and its method for preparing bioactive feed
CN108441452A (en) A kind of store pig compound microbial culture starter and its application
CN101228920B (en) Application of microecologics prepared from bacillus laterosporus WY9701 on feed additive
CN108277188A (en) A kind of weanling pig compound microbial culture starter and its application
US3243299A (en) Monogastric feed concentrate containing rumen microorganisms and lactic ferment and process of preparation
CN108060107B (en) Micro-ecological preparation for remarkably reducing body fat rate of livestock and poultry and preparation method thereof
KR20140092061A (en) The fermentation method for mixed grains using novel lactic acid bacteria isolated from grains and its production methods
CN102669432A (en) Compound probiotics preparation for geese and method for preparing same
CN107041456B (en) Fermented bean dreg feed additive and preparation method and application thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant