CN106942470A - Biological fermentation feed produced using agricultural wastes and its preparation method and application - Google Patents
Biological fermentation feed produced using agricultural wastes and its preparation method and application Download PDFInfo
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- CN106942470A CN106942470A CN201710172651.2A CN201710172651A CN106942470A CN 106942470 A CN106942470 A CN 106942470A CN 201710172651 A CN201710172651 A CN 201710172651A CN 106942470 A CN106942470 A CN 106942470A
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- 238000000855 fermentation Methods 0.000 title claims abstract description 60
- 230000004151 fermentation Effects 0.000 title claims abstract description 60
- 239000002154 agricultural waste Substances 0.000 title claims abstract description 25
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 239000007787 solid Substances 0.000 claims abstract description 34
- 239000007788 liquid Substances 0.000 claims abstract description 21
- 241000894006 Bacteria Species 0.000 claims abstract description 16
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 16
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 16
- 238000004519 manufacturing process Methods 0.000 claims abstract description 16
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 15
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 15
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 12
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 12
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 12
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 9
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 9
- 235000009566 rice Nutrition 0.000 claims abstract description 9
- 239000010903 husk Substances 0.000 claims abstract description 8
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 8
- 241000196324 Embryophyta Species 0.000 claims abstract description 4
- 241000186660 Lactobacillus Species 0.000 claims abstract description 3
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 3
- 240000007594 Oryza sativa Species 0.000 claims abstract 3
- 239000002609 medium Substances 0.000 claims description 22
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 14
- 230000000694 effects Effects 0.000 claims description 13
- 108091005508 Acid proteases Proteins 0.000 claims description 12
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 7
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 7
- 229940099596 manganese sulfate Drugs 0.000 claims description 7
- 235000007079 manganese sulphate Nutrition 0.000 claims description 7
- 239000011702 manganese sulphate Substances 0.000 claims description 7
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 7
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 7
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 7
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 7
- 239000003643 water by type Substances 0.000 claims description 7
- 244000144972 livestock Species 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 241000282898 Sus scrofa Species 0.000 claims description 5
- 239000011159 matrix material Substances 0.000 claims description 3
- 101800000263 Acidic protein Proteins 0.000 claims description 2
- 102000004190 Enzymes Human genes 0.000 claims description 2
- 108090000790 Enzymes Proteins 0.000 claims description 2
- 239000001963 growth medium Substances 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 230000036039 immunity Effects 0.000 abstract description 6
- 230000007413 intestinal health Effects 0.000 abstract description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 abstract description 6
- 230000036528 appetite Effects 0.000 abstract description 4
- 235000019789 appetite Nutrition 0.000 abstract description 4
- 230000029087 digestion Effects 0.000 abstract description 4
- 108010059892 Cellulase Proteins 0.000 abstract description 3
- 241001465754 Metazoa Species 0.000 abstract description 3
- 108091005804 Peptidases Proteins 0.000 abstract description 3
- 239000004365 Protease Substances 0.000 abstract description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract description 3
- 229940106157 cellulase Drugs 0.000 abstract description 3
- 235000014655 lactic acid Nutrition 0.000 abstract description 3
- 239000004310 lactic acid Substances 0.000 abstract description 3
- 230000002708 enhancing effect Effects 0.000 abstract description 2
- -1 ferment 72h Substances 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 10
- 241000209094 Oryza Species 0.000 description 6
- 239000000758 substrate Substances 0.000 description 6
- 230000004083 survival effect Effects 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 206010012735 Diarrhoea Diseases 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000002207 metabolite Substances 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000006041 probiotic Substances 0.000 description 2
- 235000018291 probiotics Nutrition 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
- 241000927735 Penaeus Species 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000028161 membrane depolarization Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Fodder In General (AREA)
Abstract
Biological fermentation feed produced using agricultural wastes and its preparation method and application, solid medium is composed of the following components：65 75 parts of flat mushroom bacteria residue；15 20 parts of rice husk；10 20 parts of wheat bran.Inoculated plant lactobacillus, bacillus subtilis and saccharomyces cerevisiae mixing fermentation culture liquid, ferment 72h, and solid biologic fermented feed is made.The biological fermentation feed of the utilization agricultural wastes production of the present invention is rich in Lactobacillus plantarum, bacillus subtilis and saccharomyces cerevisiae viable bacteria and its tunning lactic acid, protease and cellulase, animal appetite can be improved, improve feed digestion absorptivity, enhancing development, improve intestinal health, improve immunity.
Description
Technical field
Biological technical field is recycled the invention belongs to agricultural wastes, it is more particularly to a kind of to utilize agricultural wastes production
Biological fermentation feed and its preparation method and application.
Background technology
With the fast development of agricultural, the quantity of China's agricultural wastes is being continuously increased, such as flat mushroom bacteria residue, rice husk, bran
Skin, stalk etc., high number.Depolarization small part is directly used in outside feed, is largely gone out of use, and on the other hand, with China
The fast development of breeding scale industry and intensive feed industry so that feed resource becomes increasingly in short supply, therefore, agriculture waste
Thing utilizes biofermentation technique, characteristic feed of the exploitation rich in beneficial bacterium and its tunning, fully develops, change give up into
Treasured, is promote China's agricultural resource to recycle big while solve agricultural wastes and feed resource is in short supply the problem of exist
Gesture is become.
The content of the invention
The technical problem of solution：The present invention provides biological fermentation feed and its preparation of a kind of utilization agricultural wastes production
Methods and applications, the biological fermentation feed is through using agricultural wastes as solid medium inoculated plant lactobacillus, withered grass gemma
Bacillus and saccharomyces cerevisiae mixing fermentation culture liquid, ferment at constant temperature, compared with agricultural wastes before fermentation and conventional feed, containing big
Probiotics and tunning are measured, is applied in animal productiong, appetite can be improved, feed digestion absorptivity is improved, promotes growth hair
Educate, improve intestinal health, improve immunity.
Technical scheme：A kind of preparation method of the biological fermentation feed of utilization agricultural wastes production, preparation process is：
Lactobacillus plantarum (Lactobacillus plantarum), bacillus subtilis (Bacillus subtilis) and saccharomyces cerevisiae
(Saccharomyces cerevisiae) carries out liquid fermentation and culture respectively, with agricultural after the fermentation culture mixing obtained
Solid biologic fermented feed is made in rejected material solid culture medium fermented and cultured, 25-30 DEG C of ferment at constant temperature；The solid medium
It is made up of by weight following components：65-75 parts of flat mushroom bacteria residue；15-20 parts of rice husk；10-19.5 parts of wheat bran；Acid protease
0.01-0.05 parts；0.1-0.2 parts of potassium dihydrogen phosphate；0.05-0.1 parts of magnesium sulfate；0.1-0.2 parts of manganese sulfate；Wherein acidic protein
Enzyme enzyme-activity unit is not less than 100U/g；Also containing the 2wt.% brown sugar waters for accounting for solid medium weight 10%.
It is preferred that, the OD600 values difference of above-mentioned Lactobacillus plantarum, bacillus subtilis and saccharomyces cerevisiae after liquid fermentation
For 1.8,2.2,2.6.
It is preferred that, the fermentation culture of above-mentioned Lactobacillus plantarum, bacillus subtilis and saccharomyces cerevisiae by volume 2:1:2
Mixing.
It is preferred that, above-mentioned solid medium is constituted by weight is：67 parts of flat mushroom bacteria residue；18 parts of rice husk；14.6 parts of wheat bran；
0.02 part of acid protease；0.15 part of potassium dihydrogen phosphate；0.08 part of magnesium sulfate；0.15 part of dispensing of manganese sulfate obtains solid medium,
Wherein acid protease enzyme-activity unit is not less than 100U/g, then by solid medium and 2wt.% brown sugar waters in mass ratio 1:0.1
Mix to obtain solid culture based substrate；Mixing fermentation culture liquid is inoculated with by solid medium matrix weight 1%；In 25-30 DEG C of temperature
Ferment 72h.
The biological fermentation feed that the above method is prepared.
Application of the above-mentioned biological fermentation feed in Swine Production.
Application of the above-mentioned biological fermentation feed in aquatic livestock.
Beneficial effect：What 1. the present invention was provided improves the biological fermentation feed of meat quality, probiotics and its metabolite
It is active high, the trophic component of agricultural wastes is improved, its utilization rate is improved.2. the present invention is produced using agricultural wastes
Biological fermentation feed is applied in Swine Production, improves the appetite of pig, improves feed digestion absorptivity, promotes growth hair
Educate, improve intestinal health, improve immunity.3. the biological fermentation feed that the present invention is produced using agricultural wastes is in aquatic livestock
Application in cultivation, plays promotion aquatic livestock growth, improves intestinal health, improve immunity, improve the effect of water environment.
Embodiment：
With reference to specific embodiment, the present invention is expanded on further.It should be understood that these embodiments are merely to illustrate the present invention
Rather than limitation the scope of the present invention.The experimental method of unreceipted actual conditions in the following example, generally according to conventional strip
Part, or according to the condition proposed by manufacturer.
Embodiment 1
A kind of preparation method of the biological fermentation feed of utilization agricultural wastes production, including the production of mixing fermentation culture liquid
Lactobacillus plantarum, bacillus subtilis, saccharomyces cerevisiae, are carried out liquid fermentation and culture, four kinds of bacterium OD600 values difference by method respectively
1.8,2.2,2.6 are reached, by volume 2:1:2 mixing, obtain mixing fermentation culture liquid.Again by weight, 67 parts of flat mushroom bacteria residue；
18 parts of rice husk；14.74 parts of wheat bran；0.01 part of acid protease；0.1 part of potassium dihydrogen phosphate；0.05 part of magnesium sulfate；Manganese sulfate 0.1
Part dispensing obtains solid medium, and wherein acid protease enzyme-activity unit is not less than 100U/g, and mixer is mixed 6 minutes, and mixing is equal
After even, then by solid medium and 2wt.% brown sugar waters by weight 1:0.1 mixes to obtain solid culture based substrate, by solid culture
Based substrate weight 0.5% is inoculated with mixing fermentation culture liquid, after 25-30 DEG C of temperature, fermentation 72h, obtains finished product.
Embodiment 2
A kind of preparation method of the biological fermentation feed of utilization agricultural wastes production, including the production of mixing fermentation culture liquid
Lactobacillus plantarum, bacillus subtilis, saccharomyces cerevisiae, are carried out liquid fermentation and culture, four kinds of bacterium OD600 values difference by method respectively
1.8,2.2,2.6 are reached, by volume 2:1:2 mixing, obtain mixing fermentation culture liquid.Again by weight, 67 parts of flat mushroom bacteria residue；
18 parts of rice husk；14.6 parts of wheat bran；0.02 part of acid protease；0.15 part of potassium dihydrogen phosphate；0.08 part of magnesium sulfate；Manganese sulfate 0.15
Part dispensing obtains solid medium, and wherein acid protease enzyme-activity unit is not less than 100U/g, and mixer is mixed 6 minutes, and mixing is equal
After even, then by solid medium and 2wt.% brown sugar waters by weight 1:0.1 mixes to obtain solid culture based substrate, by solid culture
Based substrate weight 1% is inoculated with mixing fermentation culture liquid, after 25-30 DEG C of temperature, fermentation 72h, obtains finished product.
Embodiment 3
A kind of preparation method of the biological fermentation feed of utilization agricultural wastes production, including the production of mixing fermentation culture liquid
Lactobacillus plantarum, bacillus subtilis, saccharomyces cerevisiae, are carried out liquid fermentation and culture by method respectively, and four kinds of bacterium OD values reach respectively
To 1.8,2.2,2.6, by volume 2:1:2 mixing, obtain mixing fermentation culture liquid.Again by weight, 67 parts of flat mushroom bacteria residue；Rice
18 parts of shell；14.45 parts of wheat bran；0.05 part of acid protease；0.2 part of potassium dihydrogen phosphate；0.1 part of magnesium sulfate；0.2 part of manganese sulfate is matched somebody with somebody
Expect to obtain solid medium, wherein acid protease enzyme-activity unit is not less than 100U/g, and mixer is mixed 6 minutes, after being well mixed,
Again by solid medium and 2wt.% brown sugar waters by weight 1:0.1 mixes to obtain solid culture based substrate, by solid medium base
Matter weight 1% is inoculated with mixing fermentation culture liquid, after 25-30 DEG C of temperature, fermentation 72h, obtains finished product.
| Active component content | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| Moisture content (%) | 41.2 | 41.5 | 41.8 |
| Crude fibre (%) | 23.2 | 20.8 | 22.5 |
| Crude protein (%) | 12.9 | 14.4 | 13.5 |
| Lactobacillus plantarum (cfu/g) | 9.0x106 | 2.0x107 | 1.0x107 |
| Bacillus subtilis (cfu/g) | 3.0x108 | 7.0x108 | 4.0x108 |
| Saccharomyces cerevisiae (cfu/g) | 8.7x107 | 1.5x108 | 1.0x108 |
| Lactic acid (%) | 0.80 | 0.95 | 0.90 |
| Protease (u/g) | 182 | 210 | 195 |
| Cellulase (u/g) | 275 | 360 | 341 |
As can be seen from the above table, the biological fermentation feed active component content height produced with embodiment 2.The embodiment
Feature is agricultural wastes solid medium Easy dosing, easily production.Fermented with mixing fermentation culture liquid, improve agricultural useless
Gurry nutritional ingredient, reduces crude fiber content in agricultural wastes, improves protein content, simultaneously containing the newborn bar of plant
Bacterium, bacillus subtilis, saccharomyces cerevisiae and its metabolite such as lactic acid, protease, cellulase activity.It is conventional with feedstuff industry
Mixed feed, which is compared, has beneficial bacterium and its metabolite activity.
Embodiment 4
Verify application effect of the embodiment 2 in Swine Production.
(1) test method：Selective body heavy phase is near, and 35 age in days DLYs are enclosed by outer ternary piglet 12, often enclose 10, totally 120.
It is randomly divided into control group and test group, every group 60 (6 circle), experimental period is 30.Test group is replaced by biological fermentation feed 10%
For perfect compound feed, biological fermentation feed:Perfect compound feed is 1 in mass ratio:9；Control group fed routine mixed feed.
On-test, is weighed with the end of, and feed consumption is recorded during experiment.
(2) testing index
Beginning counterpoise, last counterpoise, daily gain, feedstuff-meat ratio, diarrhea rate, survival rate
(3) result of the test
| Group | Beginning counterpoise/Kg | Last counterpoise/Kg | Daily gain/g | Head feeding/kg | Feedstuff-meat ratio | Diarrhea rate/% | Survival rate/% |
| Test group | 8.52±0.30 | 23.54±1.32 | 500±21a | 24.0±0.51a | 1.60±0.06a | 0.58±0.10a | 98.5 |
| Control group | 8.43±0.40 | 21.93±0.98 | 450±16b | 23.1±0.15b | 1.71±0.04b | 2.32±0.20b | 96.8 |
The female different expression significant difference (P of digital shoulder marking-up<0.05)
(4) interpretation of result
As seen from the above table, test group is compared with control group, and daily gain improves 11.1%, and significant difference (P<0.05)；
Feedstuff-meat ratio reduces 5.26%, significant difference (P<0.05)；Diarrhea rate is decreased obviously, and significant difference (P<0.05)；Survival rate
Improve.Result above shows that the biological fermentation feed that embodiment 1 is produced using agricultural wastes can improve animal appetite, improves
Feed digestion absorptivity, enhancing development improves intestinal health, improves immunity, reduces disease.
Embodiment 5
Verify application effect of the embodiment 2 in aquatic livestock
(1) test method：2 mouthfuls of pond for selecting landform consistent with sunshine condition, is set to the control pool and examination by each 15 mu
The pool is tested, each to deliver the mantissa of Penaeus Vannmei 1,200,000, experimental period is 130.The pool is tested by the fermented feed produced in embodiment 2
10% substitutes full price batch, i.e. biological fermentation feed:Full price batch is 1 in mass ratio:9；The control pool feeds conventional cooperation
Feed.Water colour is regularly observed during experiment.
(2) testing index
Per mu yield, go out pool specification, survival rate.
(3) result of the test
| Group | Per mu yield (Kg) | Go out pool specification (mantissa/Kg) | Survival rate (%) |
| Test the pool | 768 | 62 | 68.6 |
| Control sugar | 693 | 68 | 63.1 |
(4) interpretation of result
As seen from the above table, the experiment pool is compared with compareing the pool, and per mu yield is improved, and goes out pool specification greatly, survival rate is improved.Experimental period
Between regularly observe water colour, the experiment pool is compared with compareing the pool, and water colour is stable.Result above shows, useless using agricultural described in embodiment 2
The biological fermentation feed of gurry production, feeding aquatic livestock can play promotion growth, improve intestinal health, improve immunity, change
The effect of kind water environment.
Claims (7)
1. the preparation method of the biological fermentation feed produced using agricultural wastes, it is characterised in that preparation process is：Plant
Lactobacillus（Lactobacillus plantarum）, bacillus subtilis（Bacillus subtilis）And saccharomyces cerevisiae
（Saccharomyces cerevisiae）Liquid fermentation and culture is carried out respectively, with agricultural after the fermentation culture mixing obtained
Solid biologic fermented feed is made in rejected material solid culture medium fermented and cultured, 25-30 DEG C of ferment at constant temperature；The solid medium
It is made up of by weight following components：65-75 parts of flat mushroom bacteria residue；15-20 parts of rice husk；10-19.5 parts of wheat bran；Acid protease
0.01-0.05 parts；0.1-0.2 parts of potassium dihydrogen phosphate；0.05-0.1 parts of magnesium sulfate；0.1-0.2 parts of manganese sulfate；Wherein acidic protein
Enzyme enzyme-activity unit is not less than 100U/g；Also containing the 2wt.% brown sugar waters for accounting for solid medium weight 10%.
2. the preparation method of the biological fermentation feed produced according to claim 1 using agricultural wastes, it is characterised in that
The OD600 values of the Lactobacillus plantarum, bacillus subtilis and saccharomyces cerevisiae after liquid fermentation are respectively 1.8,2.2,2.6.
3. the preparation method of the biological fermentation feed produced according to claim 1 using agricultural wastes, it is characterised in that
The fermentation culture of the Lactobacillus plantarum, bacillus subtilis and saccharomyces cerevisiae by volume 2:1:2 mixing.
4. the preparation method of the biological fermentation feed produced according to claim 1 using agricultural wastes, it is characterised in that
The solid medium is constituted by weight is：67 parts of flat mushroom bacteria residue；18 parts of rice husk；14.6 parts of wheat bran；Acid protease 0.02
Part；0.15 part of potassium dihydrogen phosphate；0.08 part of magnesium sulfate；0.15 part of dispensing of manganese sulfate obtains solid medium, wherein acid protease
Enzyme-activity unit is not less than 100U/g, then by solid medium and 2wt.% brown sugar waters in mass ratio 1:0.1 mixes to obtain solid medium
Matrix；Mixing fermentation culture liquid is inoculated with by solid medium matrix weight 1%；In 25-30 DEG C of temperature fermentation 72h.
5. the biological fermentation feed that any methods described of claim 1 ~ 4 is prepared.
6. application of the biological fermentation feed described in claim 5 in Swine Production.
7. application of the biological fermentation feed described in claim 5 in aquatic livestock produces.
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Cited By (8)
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| CN108165513A (en) * | 2018-02-06 | 2018-06-15 | 南京农业大学 | A kind of composite bacteria preparation and prepare the method for compound bacteria-fermented feed using it |
| CN108185182A (en) * | 2018-01-06 | 2018-06-22 | 大连三净集团有限公司 | A kind of preparation method of TMPF biofermentation mixed feeds that can improve meat quality |
| CN108522788A (en) * | 2018-03-13 | 2018-09-14 | 魏浩峰 | A kind of the pig antibiotic-free fermented feed and fattening pannage of low cost |
| CN110305859A (en) * | 2019-07-31 | 2019-10-08 | 陕西麦可罗生物科技有限公司 | A method of biological nitrogen is produced using kasugarnycin and polyoxin bacteria residue |
| CN113317392A (en) * | 2021-07-02 | 2021-08-31 | 塔里木大学 | Method for improving conventional nutrient content of reed straw by adopting microbial treatment |
| CN113475623A (en) * | 2021-09-07 | 2021-10-08 | 山东润德生物科技有限公司 | Fermented feed and preparation method thereof |
| CN114097933A (en) * | 2021-11-26 | 2022-03-01 | 湖南百宜饲料科技有限公司 | Method for preparing functional biological feed by using feed mill by-products and product thereof |
| CN114381409A (en) * | 2022-02-24 | 2022-04-22 | 齐齐哈尔大学 | Fermented feed for improving animal production performance and/or improving feed utilization rate and application thereof |
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