CN108060107B - Micro-ecological preparation for remarkably reducing body fat rate of livestock and poultry and preparation method thereof - Google Patents
Micro-ecological preparation for remarkably reducing body fat rate of livestock and poultry and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a microecological preparation for remarkably reducing the body fat rate of livestock and poultry and a preparation method thereof, wherein the microecological preparation is prepared by drying and pulverizing a composite microbial inoculum; wherein the compound microbial inoculum is prepared by mixing and fermenting aspergillus oryzae, candida utilis, bacillus coagulans and pediococcus acidilactici to obtain the compound microbial inoculum containing beneficial bacteria and metabolites thereof. The microecological preparation provided by the invention has the effects of well reducing the body fat rate of livestock and poultry, promoting fatty acid oxidation, inhibiting the synthesis of saturated fatty acid and improving the fat metabolism function of the livestock and poultry; and can improve the antioxidant activity of livestock and poultry, thereby achieving the effects of enhancing the immune function of animal organisms and reducing the morbidity.
Description
Technical Field
The invention relates to the technical field of feed additives, in particular to a microecological preparation for remarkably reducing the body fat rate of livestock and poultry and a preparation method thereof.
Background
Excessive deposition of fat not only affects the physique of animals but also the health of eaters, and can improve the morbidity of obesity, fatty liver, coronary heart disease and the like. The fat metabolism is very important to the influence of the carcass quality of livestock and poultry. At present, means such as genetic breeding, hormone use, nutrition regulation and control and the like are generally adopted to improve the carcass quality of livestock and poultry, increase the lean meat proportion and the commercialization rate of livestock and poultry products, reduce the content of total cholesterol, improve the content of unsaturated fatty acid and improve the quality of the livestock and poultry products. But the effect is not ideal enough and the cost is high.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide the microecological preparation for remarkably reducing the body fat rate of livestock and poultry and the preparation method thereof, and the microecological preparation has the effects of well reducing the body fat rate of livestock and poultry, promoting the oxidation of fatty acid, inhibiting the synthesis of saturated fatty acid and improving the fat metabolism function of livestock and poultry; and can improve the antioxidant activity of livestock and poultry, thereby achieving the effects of enhancing the immune function of animal organisms and reducing the morbidity.
In order to achieve the purpose, the technical scheme provided by the invention is as follows:
the invention provides a microecological preparation, which is prepared by drying and pulverizing a composite microbial inoculum; the composite microbial inoculum is prepared by mixing and fermenting aspergillus oryzae, candida utilis, bacillus coagulans and pediococcus acidilactici to obtain the composite microbial inoculum containing beneficial bacteria and metabolites thereof.
In the microecological preparation, the number of live bacteria of aspergillus oryzae is 0.5-2.0 hundred million cfu/g, the number of live bacteria of candida utilis is 5-15 hundred million cfu/g, the number of live bacteria of bacillus coagulans is 10-30 hundred million cfu/g, and the number of live bacteria of pediococcus acidilactici is 10-20 hundred million cfu/g.
In the microecological preparation, the preservation number of the strain of aspergillus oryzae is CCTCC AF2014001, the preservation number of the strain of candida utilis is CCTCC AY91011, the preservation number of the strain of bacillus coagulans is CCTCC AB92066, and the preservation number of the strain of pediococcus acidilactici is CCTCC AB2013129 or CCTCC AB 204055.
The invention also protects the application of the microecological preparation in preparing feed additives.
The invention provides a preparation method of a microecological preparation, which comprises the following steps: s1: respectively fermenting and culturing aspergillus oryzae, candida utilis, bacillus coagulans and pediococcus acidilactici to obtain various liquid strains; s2: inoculating Aspergillus oryzae liquid strain in a solid fermentation culture medium, and culturing for a first preset time; then inoculating candida utilis liquid strains, and culturing for a second preset time; then inoculating a bacillus coagulans liquid strain and a pediococcus acidilactici liquid strain, and culturing for a third preset time to obtain a composite microbial inoculum rich in beneficial bacteria and metabolites thereof; s3: drying the composite microbial inoculum and then pulverizing to obtain the microecological preparation. The preservation number of the adopted aspergillus oryzae strain is CCTCC AF2014001, the preservation number of the candida utilis strain is CCTCC AY91011, the preservation number of the bacillus coagulans strain is CCTCC AB92066, and the preservation number of the pediococcus acidilactici strain is CCTCC AB2013129 or CCTCC AB 204055.
Step S1 specifically includes: culturing Aspergillus oryzae with a fermentation medium at 28-40 ℃ for 10-20 h to obtain Aspergillus oryzae liquid strain; culturing the candida utilis by using a fermentation medium at 22-32 ℃ for 24-48 h to obtain candida utilis liquid strain; culturing bacillus coagulans for 24-48 h at 28-42 ℃ by using a liquid culture medium to obtain bacillus coagulans liquid strain; and (3) culturing the pediococcus acidilactici for 18-24 hours at 37-50 ℃ by using a fermentation medium to obtain a pediococcus acidilactici liquid strain. In step S1, the fermentation medium used is a common fermentation medium for the corresponding bacterial species.
In step S2: the inoculation amount of the Aspergillus oryzae liquid strain is 2-5%, and the first preset time is 7-10 hours; the inoculation amount of the candida utilis liquid strain is 3-5%, and the second preset time is 24 hours; the inoculation amount of the bacillus coagulans liquid strain is 5-7%, the inoculation amount of the pediococcus acidilactici liquid strain is 10-15%, and the third preset time is 24-48 h; the temperature of solid fermentation culture is 22-42 ℃.
In step S2, the solid fermentation medium comprises the following raw material components by weight: 5-12% of chitosan, 1.5-5% of astragalus polysaccharide, 2-3.5% of xylan, 30-50% of corn flour, 25-35% of bran, 3.5-5% of soybean meal, 1.5-2.5% of cottonseed meal, 5-7% of molasses and 0.85% of inorganic salt.
Wherein the inorganic salt comprises (NH)4)2SO40.6％、KH2PO40.21% and MgSO40.04％。
In step S3, the drying temperature is 50 ℃, and the particle diameter of the pulverized microecological preparation is not more than 60 meshes.
The microecological preparation provided by the invention can be directly used as a feed additive, and the addition ratio is 1.0-5.0 kg/t.
The technical scheme provided by the invention has the following beneficial effects: (1) the microecological preparation provided by the invention is natural, safe, green and pollution-free, and the product can stably improve the palatability of livestock and poultry and improve the utilization rate of feed; (2) the invention optimizes the optimal proportion of four beneficial microorganism liquid strains of bacillus coagulans, aspergillus oryzae, candida utilis and pediococcus acidilactici, determines the optimal fermentation culture conditions by adjusting the content of carbon source in the fermentation culture medium and the fermentation culture time, and leads various beneficial microorganisms to reach the optimal state, thereby preparing a more efficient microecological preparation containing rich nutrient substances; the probiotics generate a large amount of lipase to reduce the fat content in the livestock and the poultry, and improve the fat metabolism of the livestock and the poultry, thereby reducing the body fat rate of the livestock and the poultry.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Detailed Description
The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only examples, and the protection scope of the present invention is not limited thereby.
The experimental procedures in the following examples are conventional unless otherwise specified. The test materials used in the following examples were purchased from a conventional store unless otherwise specified. In the quantitative tests in the following examples, three replicates were set, and the data are the mean or the mean ± standard deviation of the three replicates.
The invention provides a micro-ecological preparation, which is prepared by drying and pulverizing a composite microbial inoculum; the composite microbial inoculum is prepared by mixing and fermenting aspergillus oryzae, candida utilis, bacillus coagulans and pediococcus acidilactici to obtain the composite microbial inoculum containing beneficial bacteria and metabolites thereof;
wherein the number of live bacteria of Aspergillus oryzae is 0.5-2.0 hundred million cfu/g, the number of live bacteria of Candida utilis is 5-15 hundred million cfu/g, the number of live bacteria of Bacillus coagulans is 10-30 hundred million cfu/g, and the number of live bacteria of Pediococcus acidilactici is 10-20 hundred million cfu/g; the preservation number of the strain of aspergillus oryzae is CCTCC AF2014001, the preservation number of the strain of candida utilis is CCTCC AY91011, the preservation number of the strain of bacillus coagulans is CCTCC AB92066, and the preservation number of the strain of pediococcus acidilactici is CCTCC AB2013129 or CCTCC AB 204055.
In addition, the invention also provides a preparation method of the microecological preparation, which comprises the following steps:
s1: culturing Aspergillus oryzae with a fermentation medium at 28-40 ℃ for 10-20 h to obtain Aspergillus oryzae liquid strain; culturing the candida utilis by using a fermentation medium at 22-32 ℃ for 24-48 h to obtain candida utilis liquid strain; culturing bacillus coagulans for 24-48 h at 28-42 ℃ by using a liquid culture medium to obtain bacillus coagulans liquid strain; culturing Pediococcus acidilactici with a fermentation medium at 37-50 ℃ for 18-24 h to obtain Pediococcus acidilactici liquid strain; wherein the preservation numbers of all strains are shown as above;
s2: inoculating Aspergillus oryzae liquid strain in a solid fermentation culture medium, wherein the inoculation amount is 2-5%, and culturing for 7-10 h; then inoculating candida utilis liquid strains with the inoculation amount of 3-5%, and culturing for 24 h; inoculating a bacillus coagulans liquid strain and a pediococcus acidilactici liquid strain, wherein the inoculation amount of the bacillus coagulans liquid strain is 5-7%, the inoculation amount of the pediococcus acidilactici liquid strain is 10-15%, and culturing for 24-48 h to obtain a composite microbial agent rich in beneficial bacteria and metabolites thereof; wherein the temperature of the whole solid fermentation culture is 22-42 ℃;
the solid fermentation medium comprises the following raw material components in percentage by weight: 5-12% of chitosan, 1.5-5% of astragalus polysaccharide, 2-3.5% of xylan, 30-50% of corn flour, 25-35% of bran, 3.5-5% of soybean meal, 1.5-2.5% of cottonseed meal, 5-7% of molasses and 0.85% of inorganic salt; the inorganic salt comprises (NH)4)2SO40.6％、KH2PO40.21% and MgSO40.04％；
S3: drying the composite microbial inoculum at 50 ℃, pulverizing, and sieving with a 60-mesh sieve to obtain the microecological preparation.
The microecological preparation and the preparation method thereof provided by the present invention will be further described with reference to the following specific examples.
Example one
The embodiment provides a microecological preparation, which is prepared by drying and pulverizing a composite microbial inoculum; the composite microbial inoculum is prepared by mixing and fermenting aspergillus oryzae, candida utilis, bacillus coagulans and pediococcus acidilactici to obtain the composite microbial inoculum containing beneficial bacteria and metabolites thereof;
wherein the number of the live bacteria of aspergillus oryzae is 1.5 hundred million cfu/g, the number of the live bacteria of candida utilis is 10 hundred million cfu/g, the number of the live bacteria of bacillus coagulans is 20 hundred million cfu/g, and the number of the live bacteria of pediococcus acidilactici is 15 hundred million cfu/g; the preservation number of the strain of aspergillus oryzae is CCTCC AF2014001, the preservation number of the strain of candida utilis is CCTCC AY91011, the preservation number of the strain of bacillus coagulans is CCTCC AB92066, and the preservation number of the strain of pediococcus acidilactici is CCTCC AB 2013129.
Example two
The embodiment provides a preparation method of a microecological preparation, which comprises the following steps:
s1: culturing Aspergillus oryzae with fermentation medium at 34 deg.C for 15 hr to obtain Aspergillus oryzae liquid strain; culturing the candida utilis by using a fermentation medium at 27 ℃ for 36h to obtain candida utilis liquid strain; culturing bacillus coagulans for 36h at 35 ℃ by using a liquid culture medium to obtain bacillus coagulans liquid strain; culturing Pediococcus acidilactici with a fermentation medium at 42 deg.C for 20h to obtain Pediococcus acidilactici liquid strain; wherein the preservation number of the strain of aspergillus oryzae is CCTCC AF2014001, the preservation number of the strain of candida utilis is CCTCC AY91011, the preservation number of the strain of bacillus coagulans is CCTCC AB92066, and the preservation number of the strain of pediococcus acidilactici is CCTCC AB 2013129;
s2: inoculating Aspergillus oryzae liquid strain in solid fermentation culture medium with the inoculum size of 3%, and culturing for 8 hr; then inoculating candida utilis liquid strain with the inoculation amount of 4 percent, and culturing for 24 hours; inoculating a bacillus coagulans liquid strain and a pediococcus acidilactici liquid strain, wherein the inoculation amount of the bacillus coagulans liquid strain is 6%, and the inoculation amount of the pediococcus acidilactici liquid strain is 12%, and culturing for 36h to obtain a composite microbial agent rich in beneficial bacteria and metabolites thereof; wherein the temperature of the whole solid fermentation culture is 32 ℃;
of solid fermentation mediaThe raw material components comprise the following components in percentage by weight: 8% of chitosan, 3.6% of astragalus polysaccharide, 2.5% of xylan, 40% of corn flour, 30% of bran, 4.5% of soybean meal, 2.0% of cottonseed meal, 6% of molasses and 0.85% of inorganic salt; the inorganic salt comprises (NH)4)2SO40.6％、KH2PO40.21% and MgSO40.04％；
S3: drying the composite microbial inoculum at 50 ℃, pulverizing, and sieving with a 60-mesh sieve to obtain the microecological preparation.
EXAMPLE III
The embodiment provides a preparation method of a microecological preparation, which comprises the following steps:
s1: culturing Aspergillus oryzae with fermentation medium at 28 deg.C for 10 hr to obtain Aspergillus oryzae liquid strain; culturing the candida utilis by using a fermentation medium at 22 ℃ for 24h to obtain candida utilis liquid strain; culturing bacillus coagulans for 24 hours at 28 ℃ by using a liquid culture medium to obtain bacillus coagulans liquid strain; culturing Pediococcus acidilactici with a fermentation medium at 37 deg.C for 18h to obtain Pediococcus acidilactici liquid strain; wherein the preservation number of the strain of aspergillus oryzae is CCTCC AF2014001, the preservation number of the strain of candida utilis is CCTCC AY91011, the preservation number of the strain of bacillus coagulans is CCTCC AB92066, and the preservation number of the strain of pediococcus acidilactici is CCTCC AB 2013129;
s2: inoculating Aspergillus oryzae liquid strain in solid fermentation culture medium with inoculum size of 2%, and culturing for 7 hr; then inoculating candida utilis liquid strain with the inoculation amount of 3 percent, and culturing for 24 hours; inoculating a bacillus coagulans liquid strain and a pediococcus acidilactici liquid strain, wherein the inoculation amount of the bacillus coagulans liquid strain is 5%, and the inoculation amount of the pediococcus acidilactici liquid strain is 10%, and culturing for 24h to obtain a composite microbial inoculum rich in beneficial bacteria and metabolites thereof; wherein the temperature of the whole solid fermentation culture is 22 ℃;
the solid fermentation medium comprises the following raw material components in percentage by weight: 5% of chitosan, 1.5% of astragalus polysaccharide, 2% of xylan, 30% of corn flour, 25% of bran, 3.5% of soybean meal, 1.5% of cottonseed meal, 5% of molasses and 0.85% of inorganic salt; inorganic salt bagComprises (NH)4)2SO40.6％、KH2PO40.21% and MgSO40.04％；
S3: drying the composite microbial inoculum at 50 ℃, pulverizing, and sieving with a 60-mesh sieve to obtain the microecological preparation.
Example four
The embodiment provides a preparation method of a microecological preparation, which comprises the following steps:
s1: culturing Aspergillus oryzae with fermentation medium at 40 deg.C for 20 hr to obtain Aspergillus oryzae liquid strain; culturing the candida utilis by using a fermentation medium at 32 ℃ for 48h to obtain candida utilis liquid strain; culturing the bacillus coagulans for 48 hours at 42 ℃ by using a liquid culture medium to obtain bacillus coagulans liquid strains; culturing Pediococcus acidilactici with a fermentation medium at 50 ℃ for 24h to obtain Pediococcus acidilactici liquid strain; wherein the preservation number of the strain of aspergillus oryzae is CCTCC AF2014001, the preservation number of the strain of candida utilis is CCTCC AY91011, the preservation number of the strain of bacillus coagulans is CCTCC AB92066, and the preservation number of the strain of pediococcus acidilactici is CCTCC AB 2013129;
s2: inoculating Aspergillus oryzae liquid strain in solid fermentation culture medium with inoculum size of 5%, and culturing for 10 hr; then inoculating candida utilis liquid strain with the inoculation amount of 5 percent, and culturing for 24 hours; then inoculating a bacillus coagulans liquid strain and a pediococcus acidilactici liquid strain, wherein the inoculation amount of the bacillus coagulans liquid strain is 7 percent, and the inoculation amount of the pediococcus acidilactici liquid strain is 15 percent, and culturing for 48 hours to obtain a composite microbial agent rich in beneficial bacteria and metabolites thereof; wherein the temperature of the whole solid fermentation culture is 42 ℃;
the solid fermentation medium comprises the following raw material components in percentage by weight: 12% of chitosan, 5% of astragalus polysaccharide, 3.5% of xylan, 50% of corn flour, 35% of bran, 5% of soybean meal, 2.5% of cottonseed meal, 7% of molasses and 0.85% of inorganic salt; the inorganic salt comprises (NH)4)2SO40.6％、KH2PO40.21% and MgSO40.04％；
S3: drying the composite microbial inoculum at 50 ℃, pulverizing, and sieving with a 60-mesh sieve to obtain the microecological preparation.
Comparative example 1
The comparative example provides a method of preparing a microecological formulation comprising the steps of:
s1: culturing Aspergillus oryzae with fermentation medium at 34 deg.C for 15 hr to obtain Aspergillus oryzae liquid strain; culturing the candida utilis by using a fermentation medium at 27 ℃ for 36h to obtain candida utilis liquid strain; culturing bacillus coagulans for 36h at 35 ℃ by using a liquid culture medium to obtain bacillus coagulans liquid strain; culturing Pediococcus acidilactici with a fermentation medium at 42 deg.C for 20h to obtain Pediococcus acidilactici liquid strain; wherein the preservation number of the strain of aspergillus oryzae is CCTCC AF2014001, the preservation number of the strain of candida utilis is CCTCC AY91011, the preservation number of the strain of bacillus coagulans is CCTCC AB92066, and the preservation number of the strain of pediococcus acidilactici is CCTCC AB 2013129;
s2: inoculating aspergillus oryzae liquid strain, candida utilis liquid strain, bacillus coagulans liquid strain and pediococcus acidilactici liquid strain simultaneously in a solid fermentation culture medium, wherein the inoculation amount of the aspergillus oryzae liquid strain is 3%, the inoculation amount of the candida utilis liquid strain is 4%, the inoculation amount of the bacillus coagulans liquid strain is 6%, the inoculation amount of the pediococcus acidilactici liquid strain is 12%, and culturing for 68h to obtain a composite microbial inoculum rich in beneficial bacteria and metabolites thereof; wherein the temperature of the whole solid fermentation culture is 32 ℃;
the solid fermentation medium comprises the following raw material components in percentage by weight: 8% of chitosan, 3.6% of astragalus polysaccharide, 2.5% of xylan, 40% of corn flour, 30% of bran, 4.5% of soybean meal, 2.0% of cottonseed meal, 6% of molasses and 0.85% of inorganic salt; the inorganic salt comprises (NH)4)2SO40.6％、KH2PO40.21% and MgSO40.04％；
S3: drying the composite microbial inoculum at 50 ℃, pulverizing, and sieving with a 60-mesh sieve to obtain the microecological preparation.
The probiotics prepared in examples two to four of the present invention were systematically evaluated for their effects by functional tests, and the probiotics provided in comparative examples were used as controls.
1. Effect of microecological preparation for beef cattle for reducing fat reducing rate of livestock body fat
Test animals: 50 healthy Simmental modified cattle are selected and randomly divided into 5 groups, namely a control group and a test group.
The test method comprises the following steps: the test period is 90 days, during the test period, all test cattle freely eat and drink water, and are immunized according to a conventional immunization program, and the feeding conditions are completely the same except that the probiotics in the daily ration fed by the test cattle are different. Wherein, the control group is fed by common daily ration (the composition and the proportion of the common daily ration are shown in table 1), and the test group is fed by respectively adding the microecologics prepared in the second to fourth examples and the first comparative example into the common daily ration according to the proportion of 5.0kg/t daily ration.
And (3) test results: after 90 days of feeding, the body fat reduction rate and the liver saturated fatty acid content of the cattle are measured. The determination method refers to GB/T9695.2-2008 meat and meat product fatty acid determination. Wherein the body fat loss rate is (bovine abdominal wall fat mass of example or comparative example-bovine abdominal wall fat mass of control group)/bovine abdominal wall fat mass of control group. Specific results are shown in tables 2 and 3.
TABLE 1 common daily ration composition and ratio of beef cattle
TABLE 2 body fat loss rate of cattle
| Group of | Body fat loss rate |
| Control group | - |
| Example two | 50％ |
| EXAMPLE III | 48％ |
| Example four | 49％ |
| Comparative example 1 | 22％ |
TABLE 3 bovine liver saturated fatty acid content
| Group of | Liver saturated fatty acid content |
| Control group | 57.9％ |
| Example two | 56.2％ |
| EXAMPLE III | 56.4％ |
| Example four | 56.3％ |
| Comparative example 1 | 57.2％ |
2. Effect of microecological preparation for broiler on reducing fat reducing rate of livestock and poultry body
Test animals: 500 feathers of healthy Ross 308 broilers are selected and randomly divided into 5 groups, namely a control group and a test group.
The test method comprises the following steps: the total test period from 14 days old to 44 days old of the chicks is 30 days, all the broilers freely feed and drink water during the test period, and the immunization is carried out according to a conventional immunization program, and the feeding conditions are completely the same except that the daily ration for feeding is different. Wherein, the control group is fed by common broiler ration (the composition and the proportion of the common broiler ration are shown in table 4), and the test group is fed by respectively adding the microecologics prepared in the second to fourth examples and the first comparative example into the common broiler ration according to the proportion of 1.0kg/t ration.
And (3) test results: after 30 days of feeding, the body fat reduction rate and the liver saturated fatty acid content of the chickens were measured. Wherein the body fat loss rate is (the fat mass of the chicken abdominal wall of the example or comparative example-the fat mass of the chicken abdominal wall of the control group)/the fat mass of the chicken abdominal wall of the control group. Specific results are shown in tables 5 and 6.
Table 4 composition and ratio of common daily ration for broilers
TABLE 5 fat loss rate of chickens
| Group of | Body fat loss rate |
| Control group | - |
| Example two | 55％ |
| EXAMPLE III | 52％ |
| Example four | 54％ |
| Comparative example 1 | 28％ |
TABLE 6 Chicken liver saturated fatty acid content
| Group of | Liver saturated fatty acid content |
| Control group | 39.39％ |
| Example two | 37.02％ |
| EXAMPLE III | 37.11％ |
| Example four | 37.08％ |
| Comparative example 1 | 38.14％ |
3. Effect of microecological preparation for meat pig in reducing fat reducing rate of livestock and poultry body fat
Test animals: 50 healthy pork pigs were selected and divided into 5 groups at random, namely a control group and a test group.
The test method comprises the following steps: the test period is 60 days, all test pigs freely eat and drink water during the test period, immunization is carried out according to a conventional immunization program, and the feeding conditions are completely the same except that the microbial ecological agents in the daily ration fed by the pigs are different. Wherein, the control group is fed by common daily ration, and the test group is fed by respectively adding the micro-ecological preparations prepared in the second to fourth examples and the first comparative example into the common daily ration according to the proportion of 3.0kg/t daily ration.
And (3) test results: after 60 days of feeding, the fat loss rate of the pigs and the saturated fatty acid content of the liver were determined. Wherein the body fat loss rate is (amount of porcine abdominal wall fat in the example or comparative example-amount of porcine abdominal wall fat in the control group)/amount of porcine abdominal wall fat in the control group. Specific results are shown in tables 7 and 5.
TABLE 7 fat loss rate in pigs
| Group of | Body fat loss rate |
| Control group | - |
| Example two | 25％ |
| EXAMPLE III | 22％ |
| Example four | 25％ |
| Comparative example 1 | 9％ |
TABLE 8 pig liver saturated fatty acid content
| Group of | Liver saturated fatty acid content |
| Control group | 42.18％ |
| Example two | 41.50％ |
| EXAMPLE III | 41.53％ |
| Example four | 41.51％ |
| Comparative example 1 | 41.96％ |
It is noted that in the microecological preparation provided by the invention, the total number of beneficial live bacteria is 3 × 109-5×109cfu/g, lipase activity: 60u/g-90 u/g. Unless defined otherwise, all technical terms or sciences used hereinThe terms should be given their ordinary meaning as understood by those skilled in the art to which the invention pertains. Unless specifically stated otherwise, the relative steps, numerical expressions, and values of the components and steps set forth in these embodiments do not limit the scope of the present invention. In all examples shown and described herein, unless otherwise specified, any particular value should be construed as merely illustrative, and not restrictive, and thus other examples of example embodiments may have different values.
In the description of the present invention, it is to be understood that the terms "first", "second" and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implying any number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention, and all of the technical solutions are covered in the protective scope of the present invention.
Claims (8)
1. A microecological formulation which is characterized in that:
the microecological preparation is prepared by drying and pulverizing a composite microbial inoculum; the composite microbial inoculum is prepared by mixing and fermenting aspergillus oryzae, candida utilis, bacillus coagulans and pediococcus acidilactici to obtain the composite microbial inoculum containing beneficial bacteria and metabolites thereof;
in the microecological preparation, the number of live bacteria of aspergillus oryzae is 0.5-2.0 hundred million cfu/g, the number of live bacteria of candida utilis is 5-15 hundred million cfu/g, the number of live bacteria of bacillus coagulans is 10-30 hundred million cfu/g, and the number of live bacteria of pediococcus acidilactici is 10-20 hundred million cfu/g;
in the microecological preparation, the strain preservation number of aspergillus oryzae is CCTCCAF2014001, the strain preservation number of candida utilis is CCTCCAAY 91011, the strain preservation number of bacillus coagulans is CCTCCAB92066, and the strain preservation number of pediococcus acidilactici is CCTCCAB2013129 or CCTCCC AB 204055.
2. Use of the probiotic of claim 1 for the preparation of a feed additive.
3. The method of preparing a microecological formulation according to claim 1, comprising the steps of:
s1: respectively fermenting and culturing aspergillus oryzae, candida utilis, bacillus coagulans and pediococcus acidilactici to obtain various liquid strains;
s2: inoculating Aspergillus oryzae liquid strain in a solid fermentation culture medium, and culturing for a first preset time; then inoculating candida utilis liquid strains, and culturing for a second preset time; then inoculating a bacillus coagulans liquid strain and a pediococcus acidilactici liquid strain, and culturing for a third preset time to obtain a composite microbial inoculum rich in beneficial bacteria and metabolites thereof;
s3: and drying and pulverizing the composite microbial inoculum to obtain the microecological preparation.
4. The method for preparing a microecological formulation according to claim 3, wherein:
step S1 specifically comprises culturing Aspergillus oryzae in a fermentation medium at 28-40 deg.C for 10-20 h to obtain Aspergillus oryzae liquid strain; culturing the candida utilis by using a fermentation medium at 22-32 ℃ for 24-48 h to obtain candida utilis liquid strain; culturing bacillus coagulans for 24-48 h at 28-42 ℃ by using a liquid culture medium to obtain bacillus coagulans liquid strain; and (3) culturing the pediococcus acidilactici for 18-24 hours at 37-50 ℃ by using a fermentation medium to obtain a pediococcus acidilactici liquid strain.
5. The method for preparing a microecological formulation according to claim 3, wherein:
in the step S2, the inoculation amount of the Aspergillus oryzae liquid strain is 2-5%, and the first preset time is 7-10 hours;
the inoculation amount of the candida utilis liquid strain is 3-5%, and the second preset time is 24 hours;
the inoculation amount of the bacillus coagulans liquid strain is 5-7%, the inoculation amount of the pediococcus acidilactici liquid strain is 10-15%, and the third preset time is 24-48 h;
the temperature of solid fermentation culture is 22-42 ℃.
6. The method for preparing a microecological formulation according to claim 3, wherein:
in step S2, the solid fermentation medium includes the following raw material components by weight: 5-12% of chitosan, 1.5-5% of astragalus polysaccharide, 2-3.5% of xylan, 30-50% of corn flour, 25-35% of bran, 3.5-5% of soybean meal, 1.5-2.5% of cottonseed meal, 5-7% of molasses and 0.85% of inorganic salt.
7. The method for preparing a microecological formulation according to claim 6, wherein:
the inorganic salt comprises (NH)4)2SO40.6％、KH2PO40.21% and MgSO40.04％。
8. The method for preparing a microecological formulation according to claim 3, wherein the microorganism is selected from the group consisting of,
in step S3, the drying temperature is 50 ℃, and the particle size of the pulverized microecological preparation is not more than 60 meshes.
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| CN114601021A (en) * | 2022-03-25 | 2022-06-10 | 天津博菲德科技有限公司 | Fatty liver resisting medicine for livestock and poultry and Chinese herbal medicine combined fermentation feed additive containing same |
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| CN104041715A (en) * | 2014-06-26 | 2014-09-17 | 广州双农生物技术有限公司 | Feed additive capable of improving lean meat rate of pigs |
| CN106036040A (en) * | 2016-07-31 | 2016-10-26 | 镇江天和生物技术有限公司 | Mixed feed additive |
| CN106615621A (en) * | 2016-11-16 | 2017-05-10 | 山东众客食品有限公司 | Feed additive for improving content of polyunsaturated fatty acid in livestock meat, as well as preparation method and application of feed additive |
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| CN104041715A (en) * | 2014-06-26 | 2014-09-17 | 广州双农生物技术有限公司 | Feed additive capable of improving lean meat rate of pigs |
| CN106036040A (en) * | 2016-07-31 | 2016-10-26 | 镇江天和生物技术有限公司 | Mixed feed additive |
| CN106615621A (en) * | 2016-11-16 | 2017-05-10 | 山东众客食品有限公司 | Feed additive for improving content of polyunsaturated fatty acid in livestock meat, as well as preparation method and application of feed additive |
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