CN108034623A - A kind of compound microbial culture starter and its application - Google Patents

A kind of compound microbial culture starter and its application Download PDF

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Publication number
CN108034623A
CN108034623A CN201810105357.4A CN201810105357A CN108034623A CN 108034623 A CN108034623 A CN 108034623A CN 201810105357 A CN201810105357 A CN 201810105357A CN 108034623 A CN108034623 A CN 108034623A
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leavening
saccharomyces cerevisiae
feed
ratio
bacillus subtilis
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CN108034623B (en
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邓雪娟
王春雨
刘世杰
田子罡
陈达
蔡辉益
程善燕
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Boyide Beijing Biotechnology Co ltd
Tianjin Bofeide Science & Technology Co ltd
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Boyide Beijing Biotechnology Co ltd
Tianjin Bofeide Science & Technology Co ltd
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/33—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/169—Plantarum
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225—Lactobacillus
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Abstract

The present invention provides a kind of compound microbial culture starter and its application, the leavening includes composite thallus and carrier；Wherein, the mass ratio of the composite thallus and carrier is (1 3):(5‑10)；The composite thallus includes lactobacillus plantarum, bacillus subtilis and saccharomyces cerevisiae.The present invention screens active bacteria by science, and strain and specific support are mixed, optimize inoculating proportion and carrier additive amount, obtain a kind of novel microbial leavening, it is added in feedstuff in right amount, adjust zymotechnique flow, it is final to be prepared that quality safety is stable and nutrition-balanced new bio fermented feed, performance in layers can be effectively improved after feeding laying hen, improves egg quality, aquaculture cost is reduced, significantly improves economic benefit.

Description

A kind of compound microbial culture starter and its application
Technical field
The present invention relates to technical field of biological fermentation, relates generally to a kind of compound microbial culture starter and its application.
Background technology
For a long time, egg is the important sources that the mankind obtain protein as cheap popular animal food, It is deep to be liked by domestic and international consumer.At present, although the laying hen of high density Scale dependency produces egg yield height, single volume Greatly, but nutritive value is often not so good as the common produced egg of farmers' laying hen, meanwhile, laying rate is blindly pursued, ignores control egg The feedstuff-egg ratio of chicken production, feedstuff-egg ratio are equally the synthesis that laying hen is only improved an important factor for influencing chicken farm productivity effect Production performance could realize maximizing the benefits.The production performance of laying hen thus how is improved, improves egg quality, is always to grind Study carefully the great difficult problem that developer faces.
CN103518980A discloses a kind of preparation method and applications of laying hen feeding micro-ecological preparation, the Tiny ecosystem Preparation is mainly made of lactobacillus plantarum and bacillus subtilis, but the invention does not reduce the feedstuff-egg ratio of laying hen, and compound micro- The preparation method of ecological agent is complicated cumbersome, it is necessary to be mixed after fermented tank Zengjing Granule with the aqueous solution containing components such as pectin Microencapsulation viable bacteria powder is made in spray drying technology afterwards, then this bacterium powder is mixed with selenide of carragheen, zinc methionine and auxiliary material It is made after even.CN102067941A discloses a kind of production method of compound microbial preparation serving as fodder, by bacillus subtilis, Brewer's yeast, three kinds of strains of lactobacillus plantarum through selection and breeding formed composite bacteria, with ball milling chaff, number skin and oligoisomaltose with Good proportioning mixing, can be effectively improved the function of intestinal canal of livestock and poultry, improve food conversion ratio, and improve the micro- life of raw animal and bird intestines State balances, but the invention merely illustrates production method, has no application example.
Therefore, by varying the component of feed feed, there is provided a kind of efficient compound microbial culture starter is simultaneously used to prepare Biological fermentation feed is to improve batterylaying to lay eggs the important channel of quality and production performance, have important market value and Application prospect.
The content of the invention
In view of the deficiencies of the prior art and the market demand, the present invention provides a kind of compound microbial culture starter and its should With, fermenting microbe is screened by science, and strain and specific support are mixed, optimize inoculating proportion and carrier additive amount, obtain To a kind of novel microbial leavening, it is added in feedstuff in right amount, zymotechnique flow is adjusted, is finally prepared into Stable to quality safety and nutrition-balanced new bio fermented feed, performance in layers can be effectively improved after feeding laying hen, Improve egg quality, reduce aquaculture cost, significantly improve economic benefit.
To reach above-mentioned purpose, the present invention adopts the following technical scheme that：
In a first aspect, the present invention provides a kind of compound microbial culture starter, the leavening includes composite thallus and load Body；
Wherein, the mass ratio of the composite thallus and carrier is (1-3):(5-10), such as can be 1:5、 1:6、1:7、 1:8、1:9、1:10、2:5、2:7、1:4、2:9、3:5、1:2、3:7、3:8、1:3 or 3:10；
The composite thallus includes the combination of lactobacillus plantarum, bacillus subtilis and saccharomyces cerevisiae.
Inventor fully studies the relation of egg feedstuff composition and performance in layers during long term production, Science screening is carried out to a variety of fermenting microbes, and strain and specific support are mixed, optimizes inoculating proportion and carrier addition Amount, obtains the novel microbial leavening described in first aspect, and the ferment-fermented obtained feed nutrition is balanced, is raising After laying hen, laying rate and egg quality can be significantly improved.
Preferably, the carrier includes powdered rice hulls and/or mountain flour.
Preferably, the bacillus subtilis, is named as bacillus subtilis (Bacillus subtilis) BFC1601, It is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), address：The Chaoyang District, Beijing City North Star No. 3 Institute of Microorganism, Academia Sinica's postcodes 100101 of the institute of West Road 1, deposit number are CGMCC No.13131, preservation day Phase is on October 21st, 2016.
The lactobacillus plantarum, is named as lactobacillus plantarum (Lactobacillus Plantarum) BFC1602, preservation In China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), address：Chaoyang District, Beijing City North Star west No. 3 Institute of Microorganism, Academia Sinica's postcodes 100101 of the institute of road 1, deposit number are CGMCC No.13132, preservation date For on October 21st, 2016.
The saccharomyces cerevisiae, is named as saccharomyces cerevisiae (Saccharomyces cerevisiae) BFC1603, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), address：BeiChen West Road, Chaoyang District, BeiJing City 1 Number No. 3 Institute of Microorganism, Academia Sinica's postcodes 100101 of institute, deposit number is CGMCC No.13133, and preservation date is On October 21st, 2016.
Preferably, lactobacillus plantarum in the composite thallus：Bacillus subtilis：Saccharomyces cerevisiae is (2-4):(1-3): (3-7), in terms of Colony Forming Unit, such as can be 2:1:3、2:3:7、4:1:3、4:1:7、 2:3:3 or 4:3:7.
Preferably, in the leavening lactobacillus plantarum viable count >=3.0 × 1010CFU/g, bacillus subtilis Viable count >=2.0 × 109CFU/g, viable count >=5.0 × 10 of saccharomyces cerevisiae10CFU/g。
In the present invention, the preparation method of leavening includes the following steps：By lactobacillus plantarum, bacillus subtilis and wine Brewer yeast ferments respectively, terminates to ferment in late log phase, zymotic fluid is carried out wet thallus is collected by centrifugation, uses physiology salt Thalline is resuspended in the phosphate buffer of water or pH 6.8, and lactobacillus plantarum, bacillus subtilis and saccharomyces cerevisiae are mixed in proportion Close, obtain composite thallus, leavening is made by the drying of mixing low temp in proportion in composite thallus mixture and mountain flour, wherein planting Viable count >=3.0 × 10 of thing lactobacillus10CFU/g, viable count >=2.0 × 10 of bacillus subtilis9CFU/g, saccharomyces cerevisiae Viable count >=5.0 × 1010CFU/g；
Second aspect, the present invention provides one kind, and leavening is used to prepare feed as described in relation to the first aspect and/or feed is auxiliary Material.
The third aspect, the present invention provide a kind of ferment-fermented obtained feed using described in first aspect.
Preferably, the feed is mainly prepared by the following raw material by weight percentage：
Corn 13%-18%, peanut meal 4%-9%, sesame seed meal 3%-8%, monosodium glutamate albumen 2%-7%, whitewashing maize peel 2%-8%, corn germ cake 22%-27%, little rice bran 17%-23%, wheat bran 5%-12%, molasses 1%-5% and mountain flour 7%-12%, surplus are water.
The corn for example can be 13%, 14%, 15%, 16%, 17% or 18%.
The peanut meal for example can be 4%, 5%, 6%, 7%, 8% or 9%.
The sesame seed meal for example can be 3%, 4%, 5%, 6%, 7% or 8%.
The monosodium glutamate albumen for example can be 2%, 3%, 4%, 5%, 6% or 7%.
The whitewashing maize peel for example can be 2%, 3%, 4%, 5%, 6%, 7% or 8%.
The corn germ cake for example can be 22%, 23%, 24%, 25%, 26% or 27%.
The little rice bran for example can be 17%, 18%, 19%, 20%, 21%, 22% or 23%.
The wheat bran for example can be 5%, 6%, 7%, 8%, 9%, 10%, 11% or 12%.
The molasses for example can be 1%, 2%, 3%, 4% or 5%.
The mountain flour for example can be 7%, 8%, 9%, 10%, 11% or 12%.
Preferably, the additive amount of the compound microbial culture starter is the 0.1-0.3% of raw material total amount, such as can be 0.1%th, 0.2% or 0.3%, it is preferably 0.1%.
Fourth aspect, the present invention provide a kind of method for preparing the feed as described in the third aspect, include the following steps：
(1) leavening is weighed by 1:50 ratio is dissolved in pure water, spare as seed liquor after activation；
(2) raw material is weighed by formula ratio, material powder is mixed to get after pulverizing and sieving；
(3) clean water, molasses, seed liquor are pressed into (18-30):(1-7):The ratio mixing of (1-10), is used as bacterium after stirring Liquid is spare；
(4) bacterium material mixture is stirred into the material powder for obtaining the bacterium solution penetrating step (2) that step (3) obtains, into Row fermentation.
Preferably, the temperature of step (1) described activation is 34-36 DEG C, such as can be 33 DEG C, 34 DEG C or 35 DEG C, preferably For 35 DEG C.
Preferably, the time of step (1) described activation is 0.5-1h, such as can be 0.5h, 0.8h or 1h.
Preferably, the aperture of step (2) described sieving is 1.5-2.5mm, for example, can be 1.5mm, 2.0mm or 2.5mm, is preferably 2mm.
Preferably, the time of step (2) described mixing is 1-3min, such as can be 1min, 2min or 3min.
Preferably, the time of step (3) described stirring is 0.5-3min, for example, can be 0.5min, 1 min, 2min or 3min。
Preferably, step (3) clean water, molasses, the ratio of seed liquor are (18-30):(1-7):(1-10), example Such as can be 18:1:1、18:1:10、18:7:1、18:7:10、30:1:1、30:7:10 or 30:7:1.
Preferably, the time of step (4) described stirring is 3-5min, such as can be 3min, 4min or 5min.
Preferably, the moisture of step (4) described fermentation is 25-35%, for example, can be 25%, 28%, 30%, 32% or 35%.
Preferably, the temperature of step (4) described fermentation is 25-30 DEG C, such as can be 25 DEG C, 28 DEG C or 30 DEG C.
Preferably, the time of step (4) described fermentation is 3-7 days, such as can be 3 days, 4 days, 5 days, 6 days or 7 days.
As optimization technique method, a kind of method for preparing the feed as described in the third aspect, specifically comprises the following steps：
(1) leavening is weighed by 1:50 ratio is dissolved in pure water, and seed is used as after 34-36 DEG C of activation 0.5-1h Liquid is spare；
(2) raw material is weighed by formula ratio, 1-3min is mixed after pulverizing and sieving and obtains material powder, the aperture of sieving is 1.5-2.5mm；
(3) clean water, molasses, seed liquor are pressed into (18-30):(1-7):The ratio mixing of (1-10), stirs 0.5-3min It is spare as bacterium solution afterwards；
(4) stirring 3-5min is mixed into bacterium material in the material powder for obtaining the bacterium solution penetrating step (2) that step (3) obtains Compound, moisture control are 25-35%, and 25-30 DEG C ferments 3-7 days.
Compared with prior art, the invention has the advantages that：
(1) microbe leaven provided by the invention screens fermenting microbe by science, and by strain and specific support phase Mixing, optimizes inoculating proportion and carrier additive amount, obtains novel microbial leavening, the ferment-fermented obtained feed battalion Equilibrium is supported, after laying hen is raised, laying rate and egg quality can be significantly improved.
(2) fermented feed raw material provided by the invention is cheap and easy to get, and production equipment input cost is low, simple production process Easy to industrialization, the nutrition of feeding quality safety and stability is balanced, during feeding laying hen, effectively improves performance in layers, improves Egg quality, reduces aquaculture cost, significantly improves economic benefit, and laying rate improves 1.29%, and average egg weight improves 0.70%, feedstuff-egg ratio reduces 10.26%, and average daily feed consumption reduces 8.39%.
Embodiment
Further to illustrate the technological means and its effect of the invention taken, below in conjunction with the preferred reality of the present invention Example is applied to further illustrate technical scheme, but the present invention is not limited in scope of embodiments.
Embodiment 1
Prepare leavening
Lactobacillus plantarum, bacillus subtilis and saccharomyces cerevisiae are fermented respectively, terminate to send out in late log phase Ferment, zymotic fluid is carried out wet thallus is collected by centrifugation, and thalline is resuspended with the phosphate buffer of physiological saline or pH 6.8, by plant Lactobacillus：Bacillus subtilis：Saccharomyces cerevisiae is 2:1:3 ratio mixing, obtains composite thallus, by composite thallus mixture 1 is pressed with powdered rice hulls:Viable count >=3.0 × 10 of leavening, wherein lactobacillus plantarum are made in the drying of 5 ratio mixing low temps10CFU/ G, viable count >=2.0 × 10 of bacillus subtilis9CFU/g, viable count >=5.0 × 10 of saccharomyces cerevisiae10CFU/g。
Prepare biological fermentation feed
(1) leavening is weighed by 1:50 ratio is dissolved in pure water, standby as seed liquor after 35 DEG C of activation 0.5h With；
(2) raw material is weighed by formula ratio, 1min is mixed after pulverizing and sieving and obtains material powder, the aperture of sieving is 2mm；
Mainly it is prepared by weight percentage by the following raw material：
Corn 13%, peanut meal 4%, sesame seed meal 3%, monosodium glutamate albumen 2%, whitewashing maize peel 2%, corn germ cake 22%th, little rice bran 17%, wheat bran 5%, molasses 1% and mountain flour 7%, surplus are water, and the additive amount of compound microbial culture starter is The 0.1% of raw material total amount；
(3) clean water, molasses, seed liquor are pressed 18:1:1 ratio mixing, is used as bacterium solution spare after stirring 0.5min；
(4) 3min is stirred in the material powder for obtaining the bacterium solution penetrating step (2) that step (3) obtains to mix into bacterium material Thing, moisture control are 25%, and 25 DEG C ferment 3 days, obtain biological fermentation feed, finished product nutritional ingredient is shown in Table 1；
Table 1
Embodiment 2
Prepare leavening
Lactobacillus plantarum, bacillus subtilis and saccharomyces cerevisiae are fermented respectively, terminate to send out in late log phase Ferment, zymotic fluid is carried out wet thallus is collected by centrifugation, and thalline is resuspended with the phosphate buffer of physiological saline or pH 6.8, by plant Lactobacillus：Bacillus subtilis：Saccharomyces cerevisiae is 4:3:7 ratio mixing, obtains composite thallus, by composite thallus mixture 3 are pressed with powdered rice hulls:The obtained leavening of 10 ratio mixing low temps drying, wherein viable count >=3.0 of lactobacillus plantarum × 1010CFU/g, viable count >=2.0 × 10 of bacillus subtilis9CFU/g, viable count >=5.0 × 10 of saccharomyces cerevisiae10CFU/ g；
Prepare biological fermentation feed
(1) leavening is weighed by 1:50 ratio is dissolved in pure water, spare as seed liquor after 36 DEG C of activation 1h；
(2) raw material is weighed by formula ratio, 3min is mixed after pulverizing and sieving and obtains material powder, the aperture of sieving is 2.5mm；
Corn 18%, peanut meal 9%, sesame seed meal 8%, monosodium glutamate albumen 7%, whitewashing maize peel 8%, corn germ cake 27%th, little rice bran 23%, wheat bran 12%, molasses 5% and mountain flour 12%, surplus are water, the additive amount of compound microbial culture starter For the 0.3% of raw material total amount,
(3) clean water, molasses, seed liquor are pressed 30:7:10 ratios mix, and are used as bacterium solution spare after stirring 3min；
(4) 5min is stirred in the material powder for obtaining the bacterium solution penetrating step (2) that step (3) obtains to mix into bacterium material Thing, moisture control are 35%, and 30 DEG C ferment 7 days, obtain biological fermentation feed, nutritional ingredient is similar to Example 1.
Embodiment 3
Prepare leavening
Lactobacillus plantarum, bacillus subtilis and saccharomyces cerevisiae are fermented respectively, terminate to send out in late log phase Ferment, zymotic fluid is carried out wet thallus is collected by centrifugation, and thalline is resuspended with the phosphate buffer of physiological saline or pH 6.8, by plant Lactobacillus：Bacillus subtilis：Saccharomyces cerevisiae is 2:3:3 ratio mixing, composite thallus mixture press 2 with mountain flour:7 ratios Viable count >=3.0 × 10 of leavening, wherein lactobacillus plantarum are made in mixing low temp drying10CFU/g, bacillus subtilis Viable count >=2.0 × 109CFU/g, viable count >=5.0 × 10 of saccharomyces cerevisiae10CFU/g；
Prepare biological fermentation feed
(1) leavening is weighed by 1:50 ratio is dissolved in pure water, standby as seed liquor after 34 DEG C of activation 0.8h With；
(2) raw material is weighed by formula ratio, 2min is mixed after pulverizing and sieving and obtains material powder, the aperture of sieving is 1.5mm；
Corn 17%, peanut meal 8%, sesame seed meal 7%, monosodium glutamate albumen 6%, whitewashing maize peel 7%, corn germ cake 26%th, little rice bran 22%, wheat bran 11%, molasses 4% and mountain flour 11%, surplus are water, the additive amount of compound microbial culture starter For the 0.2% of raw material total amount；
(3) clean water, molasses, seed liquor are pressed 18:7:10 ratios mix, and are used as bacterium solution spare after stirring 2min；
(4) 4min is stirred in the material powder for obtaining the bacterium solution penetrating step (2) that step (3) obtains to mix into bacterium material Thing, moisture control are 28%, and 28 DEG C ferment 5 days, obtain biological fermentation feed, nutritional ingredient is similar to Example 1.
Comparative example 1
Compared with Example 1, in addition to lactobacillus plantarum is not contained in leavening, other conditions are identical with embodiment 1.
Comparative example 2
Compared with Example 1, in addition to bacillus subtilis is not contained in leavening, other conditions are same as Example 1.
Comparative example 3
Compared with Example 1, in addition to saccharomyces cerevisiae is not contained in leavening, other conditions are same as Example 1.
Comparative example 4
Compared with Example 1, except the ratio of three kinds of strains in leavening is changed to 1:1:Outside 1, other conditions and embodiment 1 It is identical.
Comparative example 5
Compared with Example 1, except the mass ratio of composite thallus and carrier is changed to 1:Outside 1, other conditions and 1 phase of embodiment Together.
Comparative example 6
Compared with Example 1, in addition to composite thallus additive amount is the 0.05% of raw material total amount, other conditions and embodiment 1 It is identical.
Comparative example 7
Compared with Example 1, in addition to composite thallus additive amount is the 0.5% of raw material total amount, other conditions and embodiment 1 It is identical.
Comparative example 8
Compared with Example 1, in addition to strain soak time is changed to 0.25h, other conditions are same as Example 1.
Comparative example 9
Compared with Example 1, except the time of fermentation is changed to 2 beyond the highest heavens, other conditions are same as Example 1.
Laying hen feeding evaluation
Plan design：Choose the 35 week old Hai Lan of laying period that feeding is normal, weight is close, laying rate is consistent, healthy Brown laying hen 30,000, is divided to two treatment groups, i.e. control group and test group, and each treatment group is a henhouse, every henhouse 1.5 ten thousand Only, wherein control group normally feeds basal diet, and the biological fermentation feed that test group feeding embodiment and comparative example obtain (is matched somebody with somebody Fang Jianbiao 2), it is positive to try the phase as 6 weeks, test and carried out in biological feedstuff exploitation National Engineering Research Centre breeding layer chicken Demonstration Base；
Table 2
Species Percentage (%)
Corn 62.0
Dregs of beans 22.5
Expanded soybean 5
Mountain flour 7.5
Premix 3
Biological fermentation feed Substitute 5% complete diet pellet
Feeding management：Test chicken free choice feeding, drinking-water, feeding management are performed according to Demonstration Base current programme.Statistics examination Test phase each group chicken egg production, feed consumption rate, average egg weight, feedstuff-egg ratio and laying rate etc.；
Sample collection is with preparing：An egg is collected weekly, and 10 pieces of eggs of every group of collection carry out Egg Quality index determining；
Testing index：Production performance index is laying rate, average egg weight, feedstuff-egg ratio, death and culling rate, feed consumption rate etc.；Egg product Matter index is shell thickness, eggshell strength, protein index, yolk color, hangh unit etc.；
Data processing：Statistical analysis is carried out to result using SAS statistical softwares ANOVA processes and DuncanShi methods carry out Multiple range test, is as a result represented with mean+SD, with p<0.05 is the standard for judging conspicuousness；
Production performance analysis the results are shown in Table 3；
Table 3
Note：With the female different expression significant difference (P of column data shoulder marking-up<0.05), letter is identical or represents difference without letter Not significantly (P>0.05)；
Analyzed from table 3, the biofermentation that embodiment 1-3 is produced according to condition provided by the invention and parameter is raised Material influences laying rate, average egg weight, feedstuff-egg ratio and average daily feed consumption significantly (P<0.05), the biofermentation of wherein embodiment 1 is raised Expect that the influence to production performance is optimal, laying rate improves 1.29%, and average egg weight improves 0.70%, and feedstuff-egg ratio reduces 10.26%, average daily feed consumption reduces 8.39%；Not significantly (P is influenced on death and culling rate>0.05).And used by comparative example 1-9 Working condition and parameter exceed scope provided by the invention, and production performance is had no significant effect, shows that each strain is lacked with carrier One can not, it is synergistic, lack any component or ratio not in scope provided by the invention, be unable to reach ideal effect.
Egg Quality：0-6 weeks Egg Quality data are analyzed, the results are shown in Table 4；
Table 4
Note：With the female different expression significant difference (P of column data shoulder marking-up<0.05), letter is identical or represents difference without letter Not significantly (P>0.05)；
As shown in Table 4, the biofermentation that embodiment 1-3 is produced according to parameter provided by the invention and condition and range is raised Material influences egg shape index, shell thickness, Eggshell weight, eggshell strength and yolk color not significantly (P>0.05)；To yolk height Degree, yolk diameter, albumen height and hangh unit influence significantly (P<0.05), yolk substantially becomes larger, and (yolk highly becomes higher, directly Footpath becomes larger), albumen height diminishes, and hangh unit diminishes, and the impact effect of wherein embodiment 1 is best.And comparative example 1-9 is used Strain and related proportioning and production technology exceed scope provided by the invention, the influence to egg quality is not notable, shows Each strain and carrier are indispensable, synergistic, lack any component or ratio not in scope provided by the invention, equal nothing Method reaches ideal effect.
In conclusion the present invention provides a kind of compound microbial culture starter and its application, active bacteria is screened by science Kind, and strain and specific support are mixed, optimize inoculating proportion and carrier additive amount, obtain a kind of novel microbial fermentation Agent, it is added in feedstuff in right amount, adjusts zymotechnique flow, and quality safety stabilization and nutrition is finally prepared Balanced new bio fermented feed, can effectively improve performance in layers after feeding laying hen, improve egg quality, reduce Aquaculture cost, significantly improves economic benefit.Lack any strain or strain ratio in leavening not in ratio provided by the invention Ideal effect is inside unable to reach, the additive amount of leavening and the parameter of fermentation manufacturing technique can also influence feed and laying hen given birth to Produce the raising of performance and egg quality.
Applicant states that the present invention illustrates the method detailed of the present invention, but not office of the invention by above-described embodiment It is limited to above-mentioned method detailed, that is, does not mean that the present invention has to rely on above-mentioned method detailed and could implement.Technical field Technical staff it will be clearly understood that any improvement in the present invention, equivalence replacement and auxiliary element to each raw material of product of the present invention Addition, selection of concrete mode etc., all fall within protection scope of the present invention and the open scope.

Claims (10)

1. a kind of compound microbial culture starter, it is characterised in that the leavening includes composite thallus and carrier；
Wherein, the mass ratio of the composite thallus and carrier is (1-3):(5-10)；
The composite thallus includes the combination of lactobacillus plantarum, bacillus subtilis and saccharomyces cerevisiae.
2. leavening according to claim 1, it is characterised in that the carrier includes powdered rice hulls and/or mountain flour；
Preferably, the bacillus subtilis, is named as bacillus subtilis (Bacillus subtilis) BFC1601, preservation In China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), deposit number is CGMCC No.13131, Preservation date is on October 21st, 2016；
The plant lactobacillic acid bacterium, is named as lactobacillus plantarum (Lactobacillus) BFC1602, is preserved in China Microbiological Culture presevation administration committee common micro-organisms center (CGMCC), deposit number are CGMCC No.13132, and preservation date is On October 21st, 2016；
The saccharomyces cerevisiae, is named as saccharomyces cerevisiae (Saccharomyces cerevisiae) BFC1603, and it is micro- to be preserved in China Biological inoculum preservation administration committee common micro-organisms center (CGMCC), deposit number are CGMCC No.13133, preservation date For on October 21st, 2016.
3. leavening according to claim 1 or 2, it is characterised in that lactobacillus plantarum in the composite thallus：Withered grass bud Spore bacillus：Saccharomyces cerevisiae is (2-4):(1-3):(3-7), in terms of Colony Forming Unit.
4. leavening according to any one of claim 1-3, it is characterised in that lactobacillus plantarum in the leavening Viable count >=3.0 × 1010CFU/g, viable count >=2.0 × 10 of bacillus subtilis9CFU/g, the viable count of saccharomyces cerevisiae >= 5.0×1010CFU/g。
5. a kind of leavening as any one of claim 1-4 is used to prepare feed and/or feed supplementation.
6. a kind of feed, it is characterised in that obtained using ferment-fermented as any one of claim 1-4.
7. feed according to claim 6, it is characterised in that the feed is by weight percentage mainly by the following raw material system It is standby to obtain：
Corn 13%-18%, peanut meal 4%-9%, sesame seed meal 3%-8%, monosodium glutamate albumen 2%-7%, whitewashing maize peel 2%- 8%th, corn germ cake 22%-27%, little rice bran 17%-23%, wheat bran 5%-12%, molasses 1%-5% and mountain flour 7%- 12%, surplus is water.
Preferably, the additive amount of the compound microbial culture starter is the 0.1-0.3% of raw material total amount, is preferably 0.1%.
A kind of 8. method for preparing feed as claimed in claims 6 or 7, it is characterised in that include the following steps：
(1) leavening is weighed by 1:50 ratio is dissolved in pure water, spare as seed liquor after activation；
(2) raw material is weighed by formula ratio, material powder is mixed to get after pulverizing and sieving；
(3) clean water, molasses, seed liquor are pressed into (18-30):(1-7):The ratio mixing of (1-10), it is standby as bacterium solution after stirring With；
(4) bacterium material mixture is stirred into the material powder for obtaining the bacterium solution penetrating step (2) that step (3) obtains, is sent out Ferment.
9. according to the method described in claim 8, it is characterized in that, the temperature of step (1) described activation be 34-36 DEG C, it is excellent Elect 35 DEG C as；
Preferably, the time of step (1) described activation is 0.5-1h；
Preferably, the aperture of step (2) described sieving is 1.5-2.5mm, is preferably 2mm；
Preferably, the time of step (2) described mixing is 1-3min；
Preferably, the time of step (3) described stirring is 0.5-3min；
Preferably, the time of step (4) described stirring is 3-5min；
Preferably, the moisture of step (4) described fermentation is 25-35%；
Preferably, the temperature of step (4) described fermentation is 25-30 DEG C；
Preferably, the time of step (4) described fermentation is 3-7 days.
10. method according to claim 8 or claim 9, it is characterised in that specifically comprise the following steps：
(1) leavening is weighed by 1:50 ratio is dissolved in pure water, standby as seed liquor after 34-36 DEG C of activation 0.5-1h With；
(2) raw material is weighed by formula ratio, 1-3min is mixed after pulverizing and sieving and obtains material powder, the aperture of sieving is 1.5- 2.5mm；
(3) clean water, molasses, seed liquor are pressed into (18-30):(1-7):The ratio mixing of (1-10), makees after stirring 0.5-3min It is spare for bacterium solution；
(4) bacterium solution that step (3) obtains is sprayed into the material powder that step (2) obtains and stirs 3-5min into bacterium material mixture, Moisture control is 25-35%, and 25-30 DEG C ferments 3-7 days.
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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108998391A (en) * 2018-08-08 2018-12-14 博益德（北京）生物科技有限公司 A kind of compound microbial culture starter, the biological fermentation feed prepared using the leavening and preparation method thereof
CN109370960A (en) * 2018-12-24 2019-02-22 中国农业科学院饲料研究所 A kind of laying hen compound microbial culture starter and its application
CN111218440A (en) * 2019-11-29 2020-06-02 博益德（北京）生物科技有限公司 Compound fermentation inoculant for livestock and poultry as well as preparation method and application thereof
CN111227111A (en) * 2020-01-14 2020-06-05 齐齐哈尔大学 Microbial fermentation feed and application
CN111436526A (en) * 2020-04-01 2020-07-24 浙江大学 Preparation method and application of fermented rice bran meal with bacterium enzyme for improving growth performance of fattening pigs
CN112806498A (en) * 2021-01-21 2021-05-18 乐山市农业科学研究院 Laying hen tea residue bacterium enzyme synergistic fermentation feed and preparation method thereof
CN113115858A (en) * 2019-12-31 2021-07-16 内蒙古工业大学 Corn bran mycoprotein fermentation feed and preparation method thereof
CN114381409A (en) * 2022-02-24 2022-04-22 齐齐哈尔大学 Fermented feed for improving animal production performance and/or improving feed utilization rate and application thereof
CN115474656A (en) * 2022-10-28 2022-12-16 丰县新中牧饲料有限公司 Laying fowl feed additive and preparation method and use method thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103766650A (en) * 2013-12-27 2014-05-07 金湖县安农生态农业发展有限公司 Laying hen feed additive and preparation thereof
CN103960468A (en) * 2014-03-28 2014-08-06 山西晋龙集团饲料有限公司 Chicken feed and production technology thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103766650A (en) * 2013-12-27 2014-05-07 金湖县安农生态农业发展有限公司 Laying hen feed additive and preparation thereof
CN103960468A (en) * 2014-03-28 2014-08-06 山西晋龙集团饲料有限公司 Chicken feed and production technology thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LOH ET AL.: "Effects of feeding different postbiotic metabolite combinations produced by Lactobacillus plantarum strains on egg quality and production performance, faecal parameters and plasma cholesterol in laying hens", 《BMC VETERINARY RESEARCH》 *
朱丹: "益生菌的功能及其在畜禽生产中的应用", 《现代畜牧兽医》 *

Cited By (12)

* Cited by examiner, † Cited by third party
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CN108998391A (en) * 2018-08-08 2018-12-14 博益德（北京）生物科技有限公司 A kind of compound microbial culture starter, the biological fermentation feed prepared using the leavening and preparation method thereof
CN108998391B (en) * 2018-08-08 2021-12-28 博益德（北京）生物科技有限公司 Composite microbial fermentation agent, biological fermentation feed prepared by using fermentation agent and preparation method of biological fermentation feed
CN109370960A (en) * 2018-12-24 2019-02-22 中国农业科学院饲料研究所 A kind of laying hen compound microbial culture starter and its application
CN111218440A (en) * 2019-11-29 2020-06-02 博益德（北京）生物科技有限公司 Compound fermentation inoculant for livestock and poultry as well as preparation method and application thereof
CN111218440B (en) * 2019-11-29 2022-06-24 博益德（北京）生物科技有限公司 Compound fermentation inoculant for livestock and poultry as well as preparation method and application thereof
CN113115858A (en) * 2019-12-31 2021-07-16 内蒙古工业大学 Corn bran mycoprotein fermentation feed and preparation method thereof
CN111227111A (en) * 2020-01-14 2020-06-05 齐齐哈尔大学 Microbial fermentation feed and application
CN111436526A (en) * 2020-04-01 2020-07-24 浙江大学 Preparation method and application of fermented rice bran meal with bacterium enzyme for improving growth performance of fattening pigs
CN112806498A (en) * 2021-01-21 2021-05-18 乐山市农业科学研究院 Laying hen tea residue bacterium enzyme synergistic fermentation feed and preparation method thereof
CN114381409A (en) * 2022-02-24 2022-04-22 齐齐哈尔大学 Fermented feed for improving animal production performance and/or improving feed utilization rate and application thereof
CN114381409B (en) * 2022-02-24 2023-08-18 齐齐哈尔大学 Fermented feed for improving animal productivity and/or improving feed utilization rate and application thereof
CN115474656A (en) * 2022-10-28 2022-12-16 丰县新中牧饲料有限公司 Laying fowl feed additive and preparation method and use method thereof

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