CN110506831A - Compoiste fermented feed and its preparation and application method for prevention of diarrhea in piglets - Google Patents
Compoiste fermented feed and its preparation and application method for prevention of diarrhea in piglets Download PDFInfo
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- CN110506831A CN110506831A CN201910646945.3A CN201910646945A CN110506831A CN 110506831 A CN110506831 A CN 110506831A CN 201910646945 A CN201910646945 A CN 201910646945A CN 110506831 A CN110506831 A CN 110506831A
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- 206010012735 Diarrhoea Diseases 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims abstract description 15
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 230000002265 prevention Effects 0.000 title claims abstract description 12
- 238000000855 fermentation Methods 0.000 claims abstract description 58
- 230000004151 fermentation Effects 0.000 claims abstract description 58
- 239000007788 liquid Substances 0.000 claims abstract description 20
- 230000000694 effects Effects 0.000 claims abstract description 19
- 241000191996 Pediococcus pentosaceus Species 0.000 claims abstract description 16
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 16
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 16
- 230000003115 biocidal effect Effects 0.000 claims abstract description 14
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 14
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 13
- 240000008042 Zea mays Species 0.000 claims abstract description 12
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 12
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 12
- 235000005822 corn Nutrition 0.000 claims abstract description 12
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 11
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 11
- 230000000433 anti-nutritional effect Effects 0.000 claims abstract description 9
- 239000002253 acid Substances 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 241000196324 Embryophyta Species 0.000 claims abstract description 7
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 7
- 239000004310 lactic acid Substances 0.000 claims abstract description 7
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000006027 corn-soybean meal Substances 0.000 claims abstract description 6
- 239000000835 fiber Substances 0.000 claims abstract description 6
- 108010083391 glycinin Proteins 0.000 claims abstract description 6
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 6
- 239000011574 phosphorus Substances 0.000 claims abstract description 6
- 108010044091 Globulins Proteins 0.000 claims abstract description 5
- 102000006395 Globulins Human genes 0.000 claims abstract description 5
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 claims abstract description 5
- UQZIYBXSHAGNOE-USOSMYMVSA-N Stachyose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO[C@@H]2[C@@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O2)O1 UQZIYBXSHAGNOE-USOSMYMVSA-N 0.000 claims abstract description 5
- 230000007423 decrease Effects 0.000 claims abstract description 5
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- UQZIYBXSHAGNOE-XNSRJBNMSA-N stachyose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO[C@@H]3[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O3)O)O2)O)O1 UQZIYBXSHAGNOE-XNSRJBNMSA-N 0.000 claims abstract description 5
- 238000003756 stirring Methods 0.000 claims abstract description 5
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 claims abstract description 4
- MUPFEKGTMRGPLJ-RMMQSMQOSA-N Raffinose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 MUPFEKGTMRGPLJ-RMMQSMQOSA-N 0.000 claims abstract description 4
- MUPFEKGTMRGPLJ-UHFFFAOYSA-N UNPD196149 Natural products OC1C(O)C(CO)OC1(CO)OC1C(O)C(O)C(O)C(COC2C(C(O)C(O)C(CO)O2)O)O1 MUPFEKGTMRGPLJ-UHFFFAOYSA-N 0.000 claims abstract description 4
- 230000003247 decreasing effect Effects 0.000 claims abstract description 4
- 229940068041 phytic acid Drugs 0.000 claims abstract description 4
- 239000000467 phytic acid Substances 0.000 claims abstract description 4
- MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 claims abstract description 4
- 239000001963 growth medium Substances 0.000 claims description 19
- 235000019621 digestibility Nutrition 0.000 claims description 13
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 12
- 241000894006 Bacteria Species 0.000 claims description 10
- 210000001630 jejunum Anatomy 0.000 claims description 10
- 239000000843 powder Substances 0.000 claims description 9
- 210000002784 stomach Anatomy 0.000 claims description 7
- 235000019750 Crude protein Nutrition 0.000 claims description 6
- 244000005700 microbiome Species 0.000 claims description 6
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims description 5
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 238000009630 liquid culture Methods 0.000 claims description 4
- 239000012533 medium component Substances 0.000 claims description 4
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 4
- 229910000162 sodium phosphate Inorganic materials 0.000 claims description 4
- 239000007836 KH2PO4 Substances 0.000 claims description 3
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 3
- 240000003768 Solanum lycopersicum Species 0.000 claims description 3
- 241000282887 Suidae Species 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 235000012054 meals Nutrition 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 2
- 235000005744 Bacillus subtilis subsp subtilis Nutrition 0.000 claims description 2
- 241000948854 Bacillus subtilis subsp. subtilis Species 0.000 claims description 2
- 239000002054 inoculum Substances 0.000 claims description 2
- 102000004190 Enzymes Human genes 0.000 description 13
- 108090000790 Enzymes Proteins 0.000 description 13
- 239000002609 medium Substances 0.000 description 9
- 108091007433 antigens Proteins 0.000 description 6
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- 239000002994 raw material Substances 0.000 description 6
- 244000068988 Glycine max Species 0.000 description 5
- 235000010469 Glycine max Nutrition 0.000 description 5
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- 108090000145 Bacillolysin Proteins 0.000 description 3
- 244000199866 Lactobacillus casei Species 0.000 description 3
- 235000013958 Lactobacillus casei Nutrition 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 235000005911 diet Nutrition 0.000 description 3
- 230000037213 diet Effects 0.000 description 3
- 210000001198 duodenum Anatomy 0.000 description 3
- 230000006862 enzymatic digestion Effects 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 210000000936 intestine Anatomy 0.000 description 3
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- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
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- 238000012545 processing Methods 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 206010001497 Agitation Diseases 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 2
- 102000013142 Amylases Human genes 0.000 description 2
- 108010065511 Amylases Proteins 0.000 description 2
- 241000252983 Caecum Species 0.000 description 2
- 241000194032 Enterococcus faecalis Species 0.000 description 2
- 240000006024 Lactobacillus plantarum Species 0.000 description 2
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 2
- 102000035092 Neutral proteases Human genes 0.000 description 2
- 108091005507 Neutral proteases Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 238000013019 agitation Methods 0.000 description 2
- 235000019418 amylase Nutrition 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000001142 anti-diarrhea Effects 0.000 description 2
- 235000014590 basal diet Nutrition 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 210000004534 cecum Anatomy 0.000 description 2
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- 235000019784 crude fat Nutrition 0.000 description 2
- 229940032049 enterococcus faecalis Drugs 0.000 description 2
- 229940072205 lactobacillus plantarum Drugs 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
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- 244000052769 pathogen Species 0.000 description 2
- 230000017854 proteolysis Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000003053 toxin Substances 0.000 description 2
- 231100000765 toxin Toxicity 0.000 description 2
- OBMBUODDCOAJQP-UHFFFAOYSA-N 2-chloro-4-phenylquinoline Chemical compound C=12C=CC=CC2=NC(Cl)=CC=1C1=CC=CC=C1 OBMBUODDCOAJQP-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000701386 African swine fever virus Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 210000004913 chyme Anatomy 0.000 description 1
- 235000012343 cottonseed oil Nutrition 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
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- 230000000593 degrading effect Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
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- 235000021050 feed intake Nutrition 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 210000004209 hair Anatomy 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 210000003405 ileum Anatomy 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 230000008979 phosphorus utilization Effects 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
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- 239000011780 sodium chloride Substances 0.000 description 1
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- 235000015921 sodium selenite Nutrition 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 230000004584 weight gain Effects 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Mycology (AREA)
- Birds (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a kind of compoiste fermented feeds and its preparation and application method for prevention of diarrhea in piglets.Corn, dregs of beans and wheat bran 4.5:4.5:1.0 in mass ratio are pre-mixed, the ZJUAF-2 fermentation liquid of mass percent 10% and 10% ZJUAF-4 fermentation liquid are added again, and add the water of mass percent 40%, initial temperature of fermenting is 25 ~ 30 DEG C, 48~72 h of fermentation time, it is primary every 5 ~ 8 h stirrings, corn-soybean meal compoiste fermented feed is obtained when feed pH is down to 4.0.Corn, dregs of beans and wheat bran use a bacillus subtilis and one plant of Pediococcus pentosaceus mixed fermentation, β-companion's globulin and glycinin content have dropped 71.94% and 82.86%, neutral detergent fiber decline 38.93%, Flatulent factors raffinose and content of stachyose have dropped 42.22% and 36.60% respectively, phytic acid phosphorus content has dropped 37.50%, other than the above anti-nutritional factors content is decreased obviously, lactic acid content improves 3.5 times, the molten albumen of acid improves 6 times, the fermented feed feeds piglet, suitable with feeding antibiotic to the control effect of grice diarrhoea.
Description
Technical field
The present invention relates to microorganism and fermentation arts, and in particular to for prevention of diarrhea in piglets compoiste fermented feed and its
Preparation and application method.
Background technique
Diarrhea is to cause the major reason of piglet death.After wean, since alimentary canal and its enzyme system are not yet physically well developed,
Piglet reduces the digestive utilization ratio of nutriment in feed, provides advantage, Jin Eryin for the breeding of pathogenic entero becteria
Send out diarrhea.In addition, when searching for food feed, antigenic substance in feed, such as the immune response of antigen protein excitable system, it lures
Send out diarrhea.Therefore, feed anti-nutritional factors is reduced, piglet is improved to the digestibility and utilization of feed nutrition substance, it is young to facilitate prevention
Diarrhea of pigs.
Feed anti-nutritional factors is effectively reduced in biofermentation, improves animal to the digestive utilization ratio of feed nutrient, improves dynamic
Object health alleviates grice diarrhoea caused by antibiotic is reduced, it has also become the emphasis of China's feed resource exploitation.But it is raw at present
Object fermented feed is many kinds of, and the difference of bacterial strain and zymotechnique causes quality to have differences.In addition, to reduce cost, it is non-
Conventional feed raw material is used for the production of fermented feed.But unconventional raw material is temporary without the product standard perfected and suitable
Recommend additive amount, lacks to toxin in unconventional raw material and anti-nutritional factors residual evaluation (Zhang Zhen is of heap of stone, 2017), fermented product
Safety and validity still need to further prove.Therefore, safely and effectively biological fermentation feed product is urgently developed.
For the anti-nutritional factors in feed of how degrading, applicant devises one in patent ZL201610728880.3
Kind avoids existing in unconventional raw material using the conventional plants such as corn, dregs of beans and wheat bran raw material as the compoiste fermented feed of substrate
Potential toxin.Meanwhile in conjunction with the gemma bar of the efficient degradation Soybean antigen protein screened in patent ZL201610728303.4
Bacteria strain prepares the compoiste fermented feed of low Soybean antigen protein, and proves that it is effectively improved by the measurement of piglet ileal digestibility
The digestibility (C. Shi, 2017) of feed amino acid.But as single batch fermentation-scale increases to ten tons or more and a variety of
Bacterial strain is used in mixed way, and oxygen content reduces rapidly inside fermentation system, and anaerobic acid-production bacterium easily forms dominant microflora, and feed acidification adds
Fastly, the digestive enzyme activity of microorganism secretion reduces, and the quality of the decline of anti-nutritional factors Degradation Level, fermented feed reduces.
Enzyme preparation is added in feed can promote the decomposition of feed macromolecular, improve feed digestibility (such as
In CN201811644468.9 and CN201811295681.3, inventor uses neutral proteinase protein degradation raw material), still
This also makes fermented feed quality by the restriction of enzyme preparation product enzyme activity, while increasing production stage and cost again.Therefore, it fills
Strain enzyme-producing performance is waved in distribution, improves the anti-nutritional factors that substrate enzyme activity will be effectively reduced in feed in feed fermentation, prevention
Grice diarrhoea reduces production cost.
Summary of the invention
For overcome the deficiencies in the prior art, the present invention provides a kind of compoiste fermented feeds for prevention of diarrhea in piglets
And its preparation and application method.
A kind of compoiste fermented feed of corn-soybean meal for prevention of diarrhea in piglets, corn, dregs of beans and wheat bran use one plant
Bacillus subtilis and one plant of Pediococcus pentosaceus mixed fermentation, β-companion's globulin and glycinin content have dropped 71.94% He
82.86%, neutral detergent fiber decline 38.93%, Flatulent factors raffinose and content of stachyose have dropped 42.22% He respectively
36.60%, phytic acid phosphorus content has dropped 37.50%, and other than the above anti-nutritional factors content is decreased obviously, lactic acid content is improved
3.5 times, the molten albumen of acid improves 6 times, and the fermented feed feeds piglet, to control effect and the feeding antibiotic phase of grice diarrhoea
When；
The bacillus subtilis belongs to bacillus subtilis subspecies,Bacillus subtilis subsp. subtilis, it is named as ZJUAF-2, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation
Date is on August 8th, 2016, and deposit number is CGMCC NO:12824；It is first public in CN106222114A for existing bacterial strain；
The Pediococcus pentosaceus belongs to Pediococcus pentosaceus category,Pediococcus pentosaceus, it is named as ZJUAF-4,
It is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Number, the deposit date is on November 21st, 2018, deposit number was CGMCC NO:16767.
A kind of preparation method of the compoiste fermented feed of corn-soybean meal, in mass ratio by corn, dregs of beans and wheat bran
4.5:4.5:1.0 premixing, then the ZJUAF-2 fermentation liquid of 10 % of mass percent and 10% ZJUAF-4 fermentation liquid are added, and
The water of mass percent 40% is added, fermentation initial temperature is 25 ~ 30 DEG C, 48~72 h of fermentation time, every 5 ~ 8 h stirrings one
It is secondary, corn-soybean meal compoiste fermented feed is obtained when feed pH is down to 4.0；
The ZJUAF-2 fermentation liquid: LB culture medium culture ZJUAF-2 to OD is used600Reach 1.2, by volume 5.0% inoculation
Enter the liquid culture medium that ferments, medium component by mass percentage, including glycerol 2.0%, bean cake powder 3.0%, NaH2PO4 0.4%、
K2HPO40.03%, 0.5 ~ 1.0 % of wheat bran, surplus are water, and fermentation liquid cultivates 24 h, neutral proteinase in 30 DEG C, 200 r/min
Vigor reaches 600 U/mL；
The ZJUAF-4 fermentation liquid: using MRS culture medium culture to OD600Reach 1.4 ~ 1.7, by volume inoculum concentration 0.1
% access fermentation liquid culture medium, medium component includes yeast powder 0.5-2.0%, glucose 0.5-2.0%, tomato meal by quality
0.5-1.0%、KH2PO40.1-0.5%, surplus are water, and 37 DEG C of 24 h of stationary culture, bacteria concentration reaches 1.0 × 109~1.0×1010
cfu/mL。
A kind of application method of the compoiste fermented feed of corn-soybean meal can mention after fermented feed feeding piglet
High piglet digestibility, prevention of diarrhea in piglets, it is 12% that fermented feed, which is dried to biodiversity ratio in 65 DEG C, and in mass ratio 10% replaces
Corn for feeding quality 5% and 5% dregs of beans, feed the piglet of 9 kg, the jejunum trypsase vigor of piglet improves 1.45 times,
Apparent digestibility of crude protein improves 2.87 percentage points, and pH reaches 4.0 in stomach, and daily gain improves 7.9%, to grice diarrhoea rate
Control effect is suitable with feeding antibiotic.
The invention has the advantages that:
1, the neutral protease vigor for improving fermentation initial stage, reaches 20 times of original technology or so, is conducive to overcome big ton
Antigen protein is degraded insufficient problem in the fermentation of position, without adding protease preparation, reduces process flow and production cost；
2, pH in piglet stomach can be reduced to 4.0 by feeding fermented feed, advantageously reduce germ invasion gastrointestinal tract；
3, compared to addition antibiotic, after adding fermented feed, the jejunum trypsase vigor of piglet improves 1.45 times, crude protein
Apparent digestibility improves 2.87 percentage points, and daily gain improves 7.9%, and the control effect of diarrhea rate is suitable with feeding antibiotic.
Detailed description of the invention
Fermentation broth enzyme activity difference before and after Fig. 1 process modification；
Forage protein Degradation Level difference before and after Fig. 2 process modification.
Specific embodiment
Below in conjunction with drawings and examples, the present invention is further elaborated.
Present invention improves over zymotechniques, improve fermented feed substrate protein enzyme activity, play Bacillus subtilis neutral
Protease improves the nutrient digestibility of feed to the degradation effect of Soybean antigen protein.In addition, what fermentation later period microorganism generated
Organic acid advantageously reduces the pH in piglet stomach, inhibits the breeding of bacterium and virus, reduces pathogen to gastral infection, such as
The pH tolerance range (3.95 ~ 10.5) of African swine fever virus, is also beneficial to prevention of diarrhea in piglets, application potential is huge.
The preparation of embodiment 1, fermented feed:
(1) preparation of fermentation medium
Bacillus subtilis ZJUAF-2 fermentation medium: glycerol 2.0% ~ 4.0%, bean cake powder 2.0% ~ 4.0%, NaH2PO4 0.4%、
K2HPO40.03% and wheat bran 1.0%；Pediococcus pentosaceus ZJUAF-4 fermentation medium: yeast powder 0.5-2.0%, glucose 0.5-
2.0%, tomato meal 0.5-1.0% and KH2PO4 0.1-0.5%。
(2) preparation of fermentation liquid
After the activated culture with purifying of bacillus subtilis ZJUAF-2, chooses single bacterium and fall within LB culture medium, 37 DEG C, 150 rpm, train
12 h are supported, to OD600When value reaches 1.0 or so, it is inoculated with by 0.1% into fermentation medium, in 37 DEG C, 200 rpm, air agitation is trained
24 h are supported to get bacillus subtilis fermentation liquor is arrived, bacillus subtilis viable count should reach 1.0 × 108~1.0×1010
Cfu/mL, neutral proteinase enzyme activity reach 600 U/mL；After the activated culture with purifying of Pediococcus pentosaceus ZJUAF-4, list is chosen
Bacterium colony is in MRS culture medium, 24 h of stationary culture, by the seed liquor after activation by 0.1% access fermentation medium, stationary culture 24
H, it is primary every 12 h gentle agitations, keep fermentation liquid uniform.Bacteria concentration reaches 1.0 × 109~1.0×1010When cfu/mL, obtain
Pediococcus pentosaceus fermentation liquid.
(3) preparation of compoiste fermented feed
Corn, dregs of beans and wheat bran 4.5:4.5:1 in mass ratio are pre-mixed.10% is added respectively by mass volume ratio again
ZJUAF-2 fermentation liquid and ZJUAF-4 fermentation liquid, add 40% water, form fermentation system after stirring and evenly mixing；By above-mentioned fermentation body
25 ~ 30 DEG C of 48 ~ 72 h of fermentation are lain in, so that fermentation temperature is no more than 55 DEG C every 5 ~ 8 h stirrings, is down to 4.0 to feed pH
When obtain fermented feed.
The selection of 2 fermentation of bacillus subtilis culture medium of embodiment
Different fermentation mediums is respectively adopted, the ZJUAF-2 that inoculation volume ratio is 5%, ferment 24 h, to using Forint phenol method
Fermentation liquid neutral proteinase enzyme activity is measured, the results are shown in Table 2.Wherein, culture medium 1 is LB culture medium, and specific ingredient presses quality hundred
Divide the fish peptone than being 1%, 0.5% yeast powder, 1.0% NaCl；Culture medium 2 is the ZJUAF-2 culture medium before improving, tool
Body ingredient is 2.0% bean cake powder, 1.8% corn flour, 2.4% glucose, 0.4% NaH by mass percentage2PO4,
0.03% K2HPO4With 0.02% CaCl2；Culture medium 3 is improved ZJUAF-2 culture medium, and specific ingredient presses quality percentage
Than the bean cake powder 3.0% for 3.0%, 2.0% glycerol, 0.4% NaH2PO4, 0.03% K2HPO4With 1.0% wheat bran 1.0%.
Influence of 1. different culture medium of table to fermentation liquid producing enzyme vigor
| Fermentation medium | 24 h neutral protease vigors (Unit/mL) |
| Fermentation medium 1 | 38 |
| Fermentation medium 2 | 155 |
| Fermentation medium 3 | 611 |
As a result as shown in table 1 and Fig. 1, culture medium improvement is conducive to improve fermentation broth enzyme activity.Fermentation liquid is used for 20 tonnes of hairs
Ferment mixed feed, as a result as shown in Fig. 2, the degradation effect of process modification latter two Soybean antigen protein is reinforced, wherein described
Soybean antigen protein is glycinin and β-companion's globulin.
Influence of the different lactic acid bacterias of example 3 to fermented feed quality
Enterococcus faecalis, plant cream are replaced in the selection link of Pediococcus pentosaceus referring to the fermented feed preparation process of example 1
Bacillus and Lactobacillus casei.The results are shown in Table 2, after compatibility, the molten albumen of acid of Pediococcus pentosaceus ZJUAF-4 fermented feed
Content is higher compared with other bacterial strains, although feed acidity is not so good as lactobacillus plantarum and Lactobacillus casei, difference is not significant.Cause
This, Pediococcus pentosaceus ZJUAF-4 is chosen as the bacterial strain that the present invention uses.
Influence of the different lactic acid bacterias of table 2. to the molten albumen of tunning acid and pH
| Fermentation strain | The molten albumen of acid | pH |
| Pediococcus pentosaceus ZJUAF-4 | 8.85 | 3.95 |
| Enterococcus faecalis | 8.82 | 4.21 |
| Lactobacillus plantarum | 7.63 | 3.83 |
| Lactobacillus casei | 7.72 | 3.85 |
The fermentation of example 4 front and back feed chemical composition analysis
As shown in Table 3, fermented feed (corn 45%, dregs of beans 45%, wheat bran 10%) produced according to the present invention, β-companion's ball egg in feed
White and glycinin has dropped 71.94% and 82.86% respectively, and neutral detergent fiber has dropped 38.93%, Flatulent factors cottonseed
Sugar and content of stachyose have dropped 42.22% and 36.60% respectively, and phytic acid phosphorus content has dropped 37.50%, in addition to the above anti-nutrition
Factor content is decreased obviously outer, 3.5 times of lactic acid content raising, and molten 6 times of albumen raising of acid or more, the nutritive value of feed is changed
It is kind.
The fermentation of table 3. front and back feed chemical composition analysis
| Index | Non- fermented feed | Fermented feed |
| Crude protein, % | 24.03 | 27.61 |
| The molten albumen of acid, % | 1.18 | 8.85 |
| β-companion's globulin, mg/g | 31.76 | 8.91 |
| Glycinin, mg/g | 63.74 | 10.92 |
| Crude fat, % | 3.67 | 3.37 |
| Neutral detergent fiber, % | 13.64 | 8.33 |
| Acid detergent fiber, % | 3.49 | 3.58 |
| Starch, % | 30.35 | 27.48 |
| Raffinose, % | 0.36 | 0.19 |
| Stachyose, % | 2.65 | 1.68 |
| Phytate phosphorus, % | 0.32 | 0.20 |
| Lactic acid, % | 1.27 | 4.45 |
Influence of 5 fermented feed of embodiment to piglet growth performance
Test chooses sodium selenite 216 of 9.25 ± 0.68 kg, is randomly divided into two processing groups, each group of 6 repetitions,
18 piglets of each repetition.Antibiotic group: basal diet (100 mg/kg olaquindoxes of addition and 50 mg/kg kitasamycins)；Hair
Ferment feed group: the compoiste fermented feed of basal diet (not adding antibiotic)+10%.24 d of experimental period.As shown in table 4, fermented feed
Effectively control grice diarrhoea rate, anti-diarrhea effect are suitable with addition effects of antibiotics.In addition, fermented feed group piglet feed intake
6.39% is improved, daily gain improves 7.99%(P< 0.05), growth performance is improved.
Influence of 4 fermented feed of table to piglet anti-diarrhea effect and growth performance
| Project | Antibiotic group | Fermented feed group |
| Initial weight, kg | 9.37 | 9.25 |
| End weight, kg | 18.70 | 19.32 |
| Daily gain, g | 388b | 419a |
| Daily ingestion amount, g | 595 | 633 |
| Feed consumption weight gain ratio | 1.54 | 1.51 |
| Diarrhea rate, % | 7.24 | 7.94 |
Influence of 6 fermented feed of embodiment to the full intestines apparent digestibility of piglet nutrient
On the basis of embodiment 5, experimental period the 19th to 21d, receives excrement method using complete, collects piglet daily and do not land excrement sample 200g,
Excrement covers all test pigs of same colony house, uses endogenous indicator method to measure nutriment table after 55 DEG C of dries pulverizings of excrement sample
See digestibility.The results are shown in Table 5, and piglet diet addition fermented feed significantly improves the digestion of dry matter, crude protein and phosphorus
Utilization rate.
Influence of 5 fermented feed of table to the full intestines apparent digestibility of piglet nutrient
| Project | Antibiotic group | Fermented feed group |
| Dry matter, % | 84.08b | 86.67a |
| Crude protein, % | 79.76b | 82.63a |
| Crude fat, % | 76.87 | 80.56 |
| Calcium, % | 76.19 | 82.00 |
| Phosphorus, % | 64.15b | 74.02a |
Influence of 7 fermented feed of embodiment to Jejunum of Piglets digestive enzyme activity
On the basis of embodiment 6,6 piglets are randomly selected from each processing group, measure Jejunum of Piglets content enzyme activity, as a result such as
Shown in table 6, piglet diet adds 10% fermented feed and significantly improves the vigor of Jejunum of Piglets amylase and trypsase, promotes
Enteron aisle digestive function.
Influence of 6 fermented feed of table to Jejunum of Piglets digestive enzyme activity
| Project | Antibiotic group | Fermented feed group |
| Amylase, units/mg prot | 335 | 652a |
| Trypsase, units/g prot | 19.2 | 28.0a |
| Lipase, units/g prot | 295 | 391 |
Influence of 8 fermented feed of embodiment to piglet digestive pH
In 5 experimental basis of example, 6 piglets are randomly selected from each processing group, measurement piglet Stomach duodenum, returns at jejunum
The content pH of intestines, caecum and colon, the results are shown in Table 7, the reduction of piglet diet addition fermented feed (P< 0.05) piglet stomach
Interior pH to 4.0, duodenum and jejunum pH also decrease, and are conducive to the growth and breeding for inhibiting pathogen and virus.
Influence of 7 fermented feed of table to piglet digestive chyme pH
| Project | Antibiotic group | Fermented feed group |
| Stomach | 5.06b | 4.00a |
| Duodenum | 6.06 | 5.25 |
| Jejunum | 6.18 | 5.67 |
| Ileum | 6.09 | 6.63 |
| Caecum | 5.62 | 5.73 |
| Colon | 6.10 | 5.70 |
Claims (3)
1. a kind of compoiste fermented feed for prevention of diarrhea in piglets, it is characterised in that: corn, dregs of beans and wheat bran are withered using one plant
Careless bacillus and one plant of Pediococcus pentosaceus mixed fermentation, β-companion's globulin and glycinin content have dropped 71.94% He
82.86%, neutral detergent fiber decline 38.93%, Flatulent factors raffinose and content of stachyose have dropped 42.22% He respectively
36.60%, phytic acid phosphorus content has dropped 37.50%, and other than the above anti-nutritional factors content is decreased obviously, lactic acid content is improved
3.5 times, the molten albumen of acid improves 6 times, and the fermented feed feeds piglet, to control effect and the feeding antibiotic phase of grice diarrhoea
When；
The bacillus subtilis belongs to bacillus subtilis subspecies,Bacillus subtilis subsp. subtilis, it is named as ZJUAF-2；The Pediococcus pentosaceus belongs to Pediococcus pentosaceus category,Pediococcus pentosaceus, it is named as ZJUAF-4, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center,
The deposit date is on November 21st, 2018, deposit number was CGMCC NO:16767.
2. it is a kind of as described in claim 1 compoiste fermented feed preparation method, it is characterised in that: by corn, dregs of beans and
Wheat bran 4.5:4.5:1.0 in mass ratio premixing, then add the ZJUAF-2 fermentation liquid and 10% of mass percent 10 %
ZJUAF-4 fermentation liquid, and the water of mass percent 40% is added, fermentation initial temperature is 25 ~ 30 DEG C, 48~72 h of fermentation time,
It is primary every 5 ~ 8 h stirrings, corn-soybean meal compoiste fermented feed is obtained when feed pH is down to 4.0；
The ZJUAF-2 fermentation liquid: LB culture medium culture ZJUAF-2 to OD is used600Reach 1.2, by volume 5.0% inoculation
Enter the liquid culture medium that ferments, medium component by mass percentage, including glycerol 2.0%, bean cake powder 3.0%, NaH2PO4 0.4%、
K2HPO40.03%, 0.5 ~ 1.0 % of wheat bran, surplus are water, and fermentation liquid cultivates 24 h, neutral proteinase in 30 DEG C, 200 r/min
Vigor reaches 600 U/mL；
The ZJUAF-4 fermentation liquid: using MRS culture medium culture to OD600Reach 1.4 ~ 1.7, by volume inoculum concentration 0.1
% access fermentation liquid culture medium, medium component includes yeast powder 0.5-2.0%, glucose 0.5-2.0%, tomato meal by quality
0.5-1.0%、KH2PO40.1-0.5%, surplus are water, and 37 DEG C of 24 h of stationary culture, bacteria concentration reaches 1.0 × 109~1.0×1010
cfu/mL。
3. a kind of application method of compoiste fermented feed as described in claim 1, it is characterised in that: feed fermented feed
After piglet, piglet digestibility can be improved, prevention of diarrhea in piglets, it is 12% that fermented feed, which is dried to biodiversity ratio in 65 DEG C, is pressed
Mass ratio 10% substitutes the corn of feeding quality 5% and 5% dregs of beans, feeds the piglet of 9 kg, the jejunum trypsase vigor of piglet
1.45 times are improved, apparent digestibility of crude protein improves 2.87 percentage points, and pH reaches 4.0 in stomach, and daily gain improves 7.9%, to son
The control effect of diarrhea of pigs rate is suitable with feeding antibiotic.
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