CN114304411A - Fermented feed capable of being fed in whole process and preparation method and application thereof - Google Patents
Fermented feed capable of being fed in whole process and preparation method and application thereof Download PDFInfo
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- CN114304411A CN114304411A CN202111413985.7A CN202111413985A CN114304411A CN 114304411 A CN114304411 A CN 114304411A CN 202111413985 A CN202111413985 A CN 202111413985A CN 114304411 A CN114304411 A CN 114304411A
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- 238000000034 method Methods 0.000 title claims abstract description 11
- 230000008569 process Effects 0.000 title claims abstract description 10
- 238000002360 preparation method Methods 0.000 title claims abstract description 9
- 241000282887 Suidae Species 0.000 claims abstract description 32
- 238000000855 fermentation Methods 0.000 claims abstract description 31
- 230000004151 fermentation Effects 0.000 claims abstract description 31
- 239000002994 raw material Substances 0.000 claims abstract description 15
- 241000193749 Bacillus coagulans Species 0.000 claims abstract description 12
- 238000009395 breeding Methods 0.000 claims abstract description 11
- 230000001488 breeding effect Effects 0.000 claims abstract description 11
- 241000191996 Pediococcus pentosaceus Species 0.000 claims abstract description 10
- 229940054340 bacillus coagulans Drugs 0.000 claims abstract description 10
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 10
- 235000016709 nutrition Nutrition 0.000 claims abstract description 6
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 5
- 239000004310 lactic acid Substances 0.000 claims abstract description 5
- 235000019750 Crude protein Nutrition 0.000 claims abstract description 4
- 238000002156 mixing Methods 0.000 claims abstract description 4
- 108700037728 Glycine max beta-conglycinin Proteins 0.000 claims abstract description 3
- 235000019784 crude fat Nutrition 0.000 claims abstract description 3
- 108010083391 glycinin Proteins 0.000 claims abstract description 3
- 239000004615 ingredient Substances 0.000 claims abstract description 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 3
- 238000003756 stirring Methods 0.000 claims abstract description 3
- 230000004913 activation Effects 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 235000019764 Soybean Meal Nutrition 0.000 claims description 7
- 239000004455 soybean meal Substances 0.000 claims description 7
- 239000001963 growth medium Substances 0.000 claims description 6
- 239000002609 medium Substances 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 235000005822 corn Nutrition 0.000 claims description 5
- 238000004321 preservation Methods 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 3
- 244000005700 microbiome Species 0.000 claims description 3
- 230000003213 activating effect Effects 0.000 claims description 2
- 239000000835 fiber Substances 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 238000009629 microbiological culture Methods 0.000 claims description 2
- 150000003839 salts Chemical class 0.000 claims description 2
- 235000012424 soybean oil Nutrition 0.000 claims description 2
- 239000003549 soybean oil Substances 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 239000008187 granular material Substances 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- -1 pH not more than 4.3 Proteins 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 238000012360 testing method Methods 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 4
- 235000014590 basal diet Nutrition 0.000 description 4
- 235000005911 diet Nutrition 0.000 description 4
- 230000037213 diet Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 206010012735 Diarrhoea Diseases 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 241000282898 Sus scrofa Species 0.000 description 3
- 239000003242 anti bacterial agent Substances 0.000 description 3
- 229940088710 antibiotic agent Drugs 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 235000021050 feed intake Nutrition 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 239000006041 probiotic Substances 0.000 description 3
- 230000000529 probiotic effect Effects 0.000 description 3
- 235000018291 probiotics Nutrition 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 230000035882 stress Effects 0.000 description 3
- 208000007407 African swine fever Diseases 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 102000014171 Milk Proteins Human genes 0.000 description 1
- 108010011756 Milk Proteins Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000006027 corn-soybean meal Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 230000003871 intestinal function Effects 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000021239 milk protein Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 235000021238 nutrient digestion Nutrition 0.000 description 1
- 230000008621 organismal health Effects 0.000 description 1
- 230000036542 oxidative stress Effects 0.000 description 1
- 230000009984 peri-natal effect Effects 0.000 description 1
- 235000015277 pork Nutrition 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
- 208000002254 stillbirth Diseases 0.000 description 1
- 231100000537 stillbirth Toxicity 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a fermented feed capable of being fed in the whole process and a preparation method and application thereof. 1) Preparing a fermentation raw material, namely mixing bacillus coagulans: mixing pediococcus pentosaceus according to the ratio of 1: 1-1: 2, inoculating, bagging, and fermenting for 3-4 days; 2) adding 10% of the mixture into wet stirring materials or granules. The fermented feed contains the following nutritional ingredients: crude protein not less than 28%, crude fat not less than 3.5%, and crude fiberNot less than 8 percent, not less than 18 percent of small peptide, pH not more than 4.3, beta-conglycinin content not more than 7 mg/g, glycinin content not more than 8.5 mg/g, lactic acid content not less than 170 mmol/Kg, viable cell number of bacillus coagulans not less than 2.3 multiplied by 108CFU/mL, the number of viable cells of Pediococcus pentosaceus is not less than 5.0 × 108CFU/mL; the above% are all weight%. The fermented feed capable of being fed in the whole process is suitable for sows, piglets, growing pigs and fattening pigs, promotes the healthy breeding of the live pigs, obviously improves the production performance of the sows, the piglets and the growing and fattening pigs, and has good application prospect.
Description
Technical Field
The invention belongs to the technical field of animal feed, and particularly relates to fermented feed capable of being fed in the whole process, and a preparation method and application thereof.
Background
The pig raising industry is an important industry related to the national civilization and plays an important role in China. However, the breeding of pigs in China currently faces a serious challenge. The African swine fever epidemic situation seriously affects the breeding of domestic pigs, the price of pork is greatly increased once, and the biological safety becomes the key point of industrial attention more and more. The antibiotics for feeding in China are completely forbidden in 7-month and 1-month 2020, and a series of problems of pig growth performance reduction, piglet mortality and diarrhea rate increase, increase of treatment medicines and the like can be caused in a short period. In addition, the nutrient digestion and absorption rate of the live pigs is low, the treatment difficulty of the excrement is high, and the difficulty of ecological breeding of the live pigs is further aggravated by the pressure of relevant environmental protection policies.
Under the condition of intensive breeding, pigs suffer different stresses in different growth stages, and the nutrition requirements, the feeding and digestion capacities and the feed conversion rate of the pigs are different in different physiological stages. Stress in the perinatal period of the sows can cause the reduction of the feeding and reproduction performances of the sows, newborn piglets are easy to suffer from the invasion of pathogenic bacteria and cause diarrhea and the like because of not developing, weaning stress of the piglets is also a great challenge in the industry, and oxidative stress and the like are easy to cause due to intensive culture in the growing and fattening stage.
The biological fermentation feed is a biological activity feed containing probiotics and metabolites thereof, and the digestion utilization rate of the feed can be improved by changing the physicochemical properties of feed substrates through microbial fermentation. Meanwhile, in the fermentation process, probiotic bacteria can be expanded and cultured, and intermediate metabolites of the probiotic bacteria are provided, so that the intestinal microorganism stability is maintained through the probiotic effect, the intestinal function and the organism immunity are improved, and the aims of feed resource development, animal organism health promotion and high-quality safe animal product production are finally achieved. Therefore, the use of the biological fermentation feed is an important measure for realizing healthy green ecological breeding of live pigs under the background of African swine fever, feed resistance prohibition and environmental protection pressure. But reports of fermented feed production and application technologies suitable for whole-course pig breeding are very limited.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a fermented feed capable of being fed in the whole process, and a preparation method and application thereof.
A preparation method of a fermented feed capable of being fed in the whole process comprises the following steps:
1) preparing fermentation raw materials
Premixing corn, soybean meal and bran raw materials according to the mass ratio of 4:4: 2-4.5: 4.5: 1;
2) preparing an activation medium
The mass M of the activation culture medium is 10-15% of the total mass of the fermentation raw materials, 2-2.4% of the total mass of the fermentation raw materials is taken, a proper amount of water is added into soybean meal powder, clear liquid is obtained by filtration, 1-1.6% of the total mass of brown sugar is added, 0.1-0.14% of the total mass of salt is added, water is supplemented to M to obtain the activation culture medium, and then the activation culture medium is averagely divided into two parts;
3) activating and fermenting seed liquid and inoculating
Adding pediococcus pentosaceus powder accounting for 0.06-0.09% of the total mass of the fermentation raw materials into one part of activation medium, standing and keeping for 3-3.5 hours to obtain pediococcus pentosaceus fermentation seed liquid, adding bacillus coagulans powder accounting for 0.10-0.12% of the total mass of the fermentation raw materials into the other part of activation medium, and standing and culturing for 3-3.5 hours to obtain bacillus coagulans fermentation seed liquid; adding water into the two fermentation seed liquids, uniformly mixing to obtain a mixed activated fermentation seed liquid, inoculating the fermentation raw material prepared in the step 1) to enable the total water content to be 35-40% of the total mass of the fermentation system, and uniformly stirring; pediococcus pentosaceus (f) mentioned abovePediococcus pentosaceus) The strain is the existing strain and is preserved in China general microbiological culture Collection center with the preservation number of CGMCC NO: 16767; bacillus coagulans bacterium (A), (B) and (C)Bacillus coagulans) Purchased from the common microorganism center of the China Committee for culture Collection of microorganisms, and the preservation number is CGMCC NO: 1.2009 has good lactic acid producing abilityForce.
4) Bagging fermentation
The fermentation system is put into a common feed bag, and is kept stand and fermented for 3 days at a constant temperature of 30 ℃, and the feed in the bag can be used when the pH value is reduced to 4.5.
A fermented mixed feed obtained by the preparation method of the fermented feed,
the fermented mixed feed comprises the following nutritional ingredients: crude protein is more than or equal to 28 percent, crude fat is more than or equal to 3.5 percent, crude fiber is more than or equal to 8 percent, small peptide is more than or equal to 18 percent, pH is less than or equal to 4.3, beta-conglycinin content is less than or equal to 7 mg/g, glycinin content is less than or equal to 8.5 mg/g, lactic acid content is more than or equal to 170 mmol/Kg, viable cell number of bacillus coagulans is more than or equal to 2.3 multiplied by 108CFU/mL, the number of viable cells of Pediococcus pentosaceus is not less than 5.. 0X 108CFU/mL; the above% are all weight%.
The application of the fermented mixed feed capable of being fed in the whole process is suitable for sows, piglets, growing pigs and fattening pigs, and promotes the healthy breeding of the live pigs, and specifically comprises the following steps:
the corn and the soybean meal in the daily ration are equally replaced by fermented mixed feed, the replacing amount accounts for 10 percent of the total weight of the daily ration, and 1.0 to 2.0 percent of soybean oil is added.
The invention has the beneficial effects that:
the same fermented feed is suitable for breeding pigs in different stages, can be used for feeding the pigs in the whole process, is simple to operate and wide in application range, can improve the production performance of the pigs in different stages, and reduces the feed cost.
Detailed Description
The invention is further illustrated by the following examples.
Example 1 Effect on sow Productivity
In the experiment, 60 healthy growing-up binary sows are selected and randomly divided into 3 treatment groups according to the principles of the sow pre-delivery period, the birth times, the fat condition, the genetic background, the breeding score and the like, wherein each group comprises 20 sows, the treatment 1 is basic diet (control group), the treatment 2 is to add 5% of fermented feed into the basic diet, and the treatment 3 is to add 10% of fermented feed into the basic diet. The test period is from 85 days of sow pregnancy to 21 days of piglet weaning. The crude protein and digestion energy of each group of feed are the same, the nutritional requirements of NRC (2012) are met, and the feed is fed in a wet-mixed mode.
As can be seen from Table 1, compared with the control group, the 10% fermented compound feed can significantly improve the feeding of sows and the weight of weaned litter of piglets, reduce the stillbirth rate, and significantly improve the milk yield and the milk protein content of the sows. (a, b represent significant differences)
Example 2 Effect on piglet Productivity
216 healthy (Du multiplied by long multiplied by big) growing pigs of 40 days old are selected for the test, the average weight is 9.25 plus or minus 0.78 kg, the feeding period is 24 days, the pigs are weighed at 25 days, and 6 piglets with similar weight are selected for each group to be slaughtered and sampled. The test animals were randomly divided into 3 treatment groups of 6 replicates each (half of a sire and a mother), with 18 pigs per replicate. The trial contained 2 diets, treatment 1 being a corn soybean meal type basal diet (control); and 2, replacing the corn and the soybean meal in the basic ration by 10% of fermented compound feed and other nitrogen, adding no antibiotics, ensuring that the nutrition level is consistent with that of the basic group ration, and feeding by using granulated feed.
As can be seen from Table 2, compared with the control group, the daily feed intake of piglets in the 10% fermented compound feed group is respectively increased by 6.39%; the daily gain is respectively improved by 6.41 percent, the feed-weight ratio is reduced by 3.82 percent, and the diarrhea of piglets is effectively controlled.
Example 3 Effect on the Productivity of growing pigs
90 healthy "Du X Long X big" growing pigs of initial body weight (40.69 + -1.16) kg were selected and randomized into 3 groups of 3 replicates each with 10 pigs each. The control group was fed basal diet, and test 1 and test 2 groups were supplemented with 5% and 10% fermented feed, respectively, in the basal diet. No antibiotics are added into the feed. Pre-run period 5 d, positive run period 34 d.
As can be seen from Table 3, the 10% fermented feed significantly increased the daily gain of growing pigs and reduced the feed-to-weight ratio.
Example 4 Effect on the Performance of fattening pigs
600 fattening pigs (Du X long X big) were divided into 3 treatment groups at random with an average initial weight of 90.53 + -0.75 kg. Each treatment was set up in 4 replicates, with each replicate containing 50 fattening pigs (male and female halves). Treatment 1 was basal diet (control); treatment 2, replacing rice bran in basic ration by 5% of nitrogen such as fermented feed; and 3, replacing rice bran and rice bran meal in the basic ration by 10% of nitrogen such as fermented feed and the like, and feeding the feed granules. The experimental period is 39 days, after the experiment is finished, the growth performance of each group is counted, and 5 fattening pigs with the average weight of 124.50 +/-2.15 kg are randomly selected for slaughtering and sampling in each group.
As can be seen from Table 4, the daily feed intake, daily gain and feed/weight ratio of each group were higher than those of the control group, and the 10% fermented feed group was significantly improved in comparison with the control groupP <0.05), particularly, the daily feed intake of the fattening pigs is improved by 3.98%, the daily gain is improved by 21.59%, and the feed-weight ratio is reduced by 14.65%.
The embodiments in the above description can be further combined or replaced, and the embodiments are only described as preferred examples of the present invention, and do not limit the concept and scope of the present invention, and various changes and modifications made to the technical solution of the present invention by those skilled in the art without departing from the design concept of the present invention belong to the protection scope of the present invention. The scope of the invention is given by the appended claims and any equivalents thereof.
Claims (3)
1. A preparation method of fermented feed capable of being fed in the whole process is characterized by comprising the following steps: the method comprises the following steps:
1) preparing fermentation raw materials
Premixing corn, soybean meal and bran raw materials according to the mass ratio of 4:4: 2-4.5: 4.5: 1;
2) preparing an activation medium
The mass M of the activation culture medium is 10-15% of the total mass of the fermentation raw materials, 2-2.4% of the total mass of the fermentation raw materials is taken, a proper amount of water is added into soybean meal powder, clear liquid is obtained by filtration, 1-1.6% of the total mass of brown sugar is added, 0.1-0.14% of the total mass of salt is added, water is supplemented to M to obtain the activation culture medium, and then the activation culture medium is averagely divided into two parts;
3) activating and fermenting seed liquid and inoculating
Adding pediococcus pentosaceus powder accounting for 0.06-0.10% of the total mass of the fermentation raw materials into one part of activation medium, standing and culturing for 3-3.5 hours to obtain pediococcus pentosaceus fermentation seed liquid, adding bacillus coagulans powder accounting for 0.10-0.12% of the total mass of the fermentation raw materials into the other part of activation medium, and standing and culturing for 3-3.5 hours to obtain bacillus coagulans fermentation seed liquid; adding water into the two fermentation seed liquids, uniformly mixing to obtain a mixed activated fermentation seed liquid, inoculating the fermentation raw material prepared in the step 1) to enable the total water content to be 35-40% of the total mass of the fermentation system, and uniformly stirring; pediococcus pentosaceus (f) mentioned abovePediococcus pentosaceus) The strain is the existing strain and is preserved in China general microbiological culture Collection center with the preservation number of CGMCC NO: 16767; bacillus coagulans bacterium (A), (B) and (C)Bacillus coagulans) Purchased from the common microorganism center of the China Committee for culture Collection of microorganisms, and the preservation number is CGMCC NO: 1.2009, has better lactic acid producing ability;
4) bagging fermentation
And (3) putting the fermentation system into a common feed bag, standing and fermenting for 3-4 days at a constant temperature of 30 ℃, and reducing the pH value of the feed in the bag to 4.0-4.5.
2. A fermented feed obtained by the preparation method of the fermented feed is characterized in that:
the fermented mixed feed comprises the following nutritional ingredients: crude protein is more than or equal to 28 percent, crude fat is more than or equal to 3.5 percent, crude fiber is more than or equal to 8 percent, small peptide is more than or equal to 18 percent, and pH is higher thanNot more than 4.3, not more than 7 mg/g of beta-conglycinin, not more than 8.5 mg/g of glycinin, not less than 170 mmol/Kg of lactic acid, not less than 2.3X 10 of viable cell number of Bacillus coagulans8CFU/mL, the number of viable cells of Pediococcus pentosaceus is not less than 5.. 0X 108CFU/mL; the above% are all weight%.
3. Use of a fermented feed according to claim 2 which is whole-feed characterized in that: the feed is suitable for sows, piglets, growing pigs and fattening pigs, and promotes healthy breeding of the live pigs, and comprises the following specific steps:
the corn and the soybean meal in the daily ration are equally replaced by fermented mixed feed, the replacing amount accounts for 10 percent of the total weight of the daily ration, and 1.0 to 2.0 percent of soybean oil is added.
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|---|---|---|---|---|
| WO2008078878A1 (en) * | 2006-12-22 | 2008-07-03 | Deuk-Sik Lee | Fermented feeds for livestock farming using lactic acid bacteria and yeast and processing method thereof |
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| CN105166422A (en) * | 2015-05-28 | 2015-12-23 | 宋志勇 | Biological fermentation feed for accelerating pig growth and preparation method thereof |
| CN106889330A (en) * | 2017-03-01 | 2017-06-27 | 海南青牧原实业有限公司 | A kind of production method of nonreactive fermented feed for fattening pigs |
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| CN109198214A (en) * | 2018-10-30 | 2019-01-15 | 上海市农业科学院 | A kind of fermented feed, preparation method and its mixed feed |
| CN110178967A (en) * | 2019-05-08 | 2019-08-30 | 浙江大学 | Fermented feed and its preparation method and application for improving meat quality |
| CN110506831A (en) * | 2019-07-17 | 2019-11-29 | 浙江大学 | Compoiste fermented feed and its preparation and application method for prevention of diarrhea in piglets |
-
2021
- 2021-11-25 CN CN202111413985.7A patent/CN114304411A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008078878A1 (en) * | 2006-12-22 | 2008-07-03 | Deuk-Sik Lee | Fermented feeds for livestock farming using lactic acid bacteria and yeast and processing method thereof |
| CN103535323A (en) * | 2013-09-29 | 2014-01-29 | 泰安巴夫巴夫农业科技有限公司 | Livestock and poultry breeding mode |
| CN105166422A (en) * | 2015-05-28 | 2015-12-23 | 宋志勇 | Biological fermentation feed for accelerating pig growth and preparation method thereof |
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| CN110506831A (en) * | 2019-07-17 | 2019-11-29 | 浙江大学 | Compoiste fermented feed and its preparation and application method for prevention of diarrhea in piglets |
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Application publication date: 20220412 |