CN103283939A - Solid fermentation preparation method for yeast protein feed - Google Patents
Solid fermentation preparation method for yeast protein feed Download PDFInfo
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- CN103283939A CN103283939A CN2012104055860A CN201210405586A CN103283939A CN 103283939 A CN103283939 A CN 103283939A CN 2012104055860 A CN2012104055860 A CN 2012104055860A CN 201210405586 A CN201210405586 A CN 201210405586A CN 103283939 A CN103283939 A CN 103283939A
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Abstract
The invention discloses a solid fermentation preparation method for yeast protein feed. The method comprises the following steps of: by taking saccharomyces cerevisiae as a fermentation strain, culturing in an optimized solid medium at the temperature of 28-30 DEG C, and performing three-stage amplification to carry out large-scale fermentation production. The method has the beneficial effects that the production process is simple and stable, the bacteria density is high, the fermentation product can be directly used without separation and purification and is easily popularized; the yeast culture is added into the pig feed, good experimental results are achieved, the digestive tract diseases can be prevented, the growth of poultry can be promoted, the weight is increased, and the yeast culture can serve as a livestock and poultry feed additive.
Description
Technical field
The present invention relates to solid fermentation engineering field, relate in particular to a kind of solid fermentation preparation method of Yeast protein feed.
Background technology
Yeast culture (Yeast Culture is called for short YC) is a kind of culture medium inoculated yeast that saccharomycete is rely and grown, product after bio-transformation of containing.Yeast culture contains abundant vitamin, mineral matter, digestive ferment, somatomedin and than complete amino acid, is that the collection nutrition and health care are the feed addictive of one.The ruminants of at first yeast culture being fed such as nineteen twenty-five Eckles, the result shows that the application of yeast culture has promoted the production performance of animal, from then on, exploitation and the application study of yeast culture is caused people's attention gradually.Existing studies show that, YC not only has tangible effect but also good effect is also arranged in nonruminant ruminant, it is nontoxic, have no side effect and noresidue, be a kind of comparatively desirable growth promotion and immune factor, abroad, a lot of about the research of YC report, and just just begun in China, its research and understanding is nowhere near.
Saccharomycete is a class unicellular microorganism, have characteristics such as individual big, strong, easily separated, the easy cultivation of omnivorousness, protein content height and metabolite are many, in today of protein feed wretched insufficiency, the extensive use of yeast and culture thereof is of great importance to China's Developing of Animal Industry and Feed Manufacturing; In medical industry, yeast and goods thereof are used for the treatment of some indigestion, and can improve and adjust the metabolic function of human body.Therefore, the production of bakers' yeast occupies an important position in yeast industry; In animal husbandry, yeast is widely used as concentrated feed to increase the protein content in the feed, to improving dressing percentage, laying rate and the galactopoiesis rate of fowl poultry, the improvement of meat and the raising of pelage quality is all had obvious effects.
Saccharomyces cerevisiae (Saccharomyces cerevisiae) claim Saccharomyces cerevisiae or budding yeast again, is a kind of generally regarded as safe microorganism (GRAS), has higher protein content and other nutritional labeling and be widely used in food and the feed.Saccharomyces cerevisiae is and mankind's relation primary yeast the most widely, not only because it is used for making food and wine brewing such as bread and steamed bun traditionally, be used as the eucaryon model organism in modern molecule and cell biology, the present invention is to use the S. cervisiae of Guangdong Microbes Inst DSMZ as fermented bacterium.
By the Yeast protein feed bacterial classification being carried out the research of culture medium prescription, pass through single factor experiment, determine that the suitableeest carbon source is for being that (contain corn flour 40 ~ 60wt%), add 2wt% glucose and 0.5wt% urea is nitrogenous source, the suitableeest inorganic salts and growth factor are respectively 0.5wt% K to basestocks with corn flour and wheat flour
2HPO
4With the 0.05wt% biotin, fermentation with this understanding, obtaining yeast count is 5.05 * 108cfu/g; On this basis to glucose, urea, K
2HPO
4And biotin carried out 4 factors, 3 horizontal quadratures tests, filters out to optimize culture medium and be: be that basestocks (contains corn flour 40 ~ 60wt%), glucose 2wt%, urea 0.5wt%, K with corn flour and wheat flour
2HPO
41.0wt% and biotin 0.01%.Optimize under the culture medium condition at this, yeast count reaches 6.58 * 108cfu/g, has improved 1.3 times than basic fermentation medium bacterium number before optimizing.
In medical industry, yeast and goods thereof are used for the treatment of some indigestion, and can improve and adjust the metabolic function of human body.Therefore, the production of bakers' yeast occupies an important position in yeast industry.In animal husbandry, yeast is widely used as concentrated feed to increase the protein content in the feed, to improving dressing percentage, laying rate and the galactopoiesis rate of fowl poultry, the improvement of meat and the raising of pelage quality is all had obvious effects.
The solid fermentation preparation method of the present Yeast protein feed that uses, technology is loaded down with trivial details, and fermentation period is long, and not only the one-tenth viable count of Pei Yanging is low, and financial cost is higher, can not satisfy people's requirement.
Summary of the invention
The solid fermentation preparation method that the purpose of this invention is to provide a kind of Yeast protein feed is to overcome the solid fermentation preparation method above shortcomings of existing Yeast protein feed.
The technical scheme that realizes the object of the invention is as follows:
A kind of solid fermentation preparation method of Yeast protein feed may further comprise the steps:
(1) actication of culture: the fermented bacterium of-80 ℃ of preservations is rule at the YPD solid plate, cultivated 16 ~ 24 hours for 30 ℃;
(2) preparation first order seed: with the saccharomyces cerevisiae bacterial classification of activation, being inoculated in the 200 mL YPD fluid nutrient mediums with oese picking one ring, triangular flask is placed shaking table, is under the condition of 30 ℃ and 250rpm in temperature, after the shaken cultivation 16 ~ 24 hours, make the first order seed bacterium;
(3) preparation solid fermentation culture medium: it is the sterilized water of 1:1 ~ 2 that the solid butt is added mass ratio, and in the pH value for stirring under the natural conditions, under 121 ℃ condition, autoclave sterilization 20min namely can be used as the solid fermentation culture medium after cooling and uses with solid medium;
(4) solid fermentation: will insert the first order seed bacterium according to 5 ~ 10% inoculum concentration through the solid fermentation culture medium of sterilization treatment, and stir, and keep that humidity will be 50 ~ 70%, temperature is under 25 ~ 30 ℃ the condition, to cultivate about 20 hours, makes secondary seed;
(5) with the secondary seed that obtains, obtain three grades of seeds according to the further solid fermentation of step (4);
(6) can further three grades of seeds be enlarged according to the further solid fermentation of step (4) and cultivate according to concrete cultivation consumption.
Further, the solid butt in the described step (3) is: be that basestocks (contains corn flour 40 ~ 60wt%), glucose 2wt%, urea 0.5wt%, K with corn flour and wheat flour
2HPO
41.0wt% and biotin 0.01wt%.
Preferably, described fermented bacterium be saccharomyces cerevisiae (
Saccharomyces cerevisiae).
Preferably, described S. cervisiae is 0.5 ~ 2wt% as the addition of feed addictive.
Beneficial effect of the present invention: with corn flour and wheat flour as basestocks, add an amount of carbon source, nitrogenous source, inorganic salts and growth factor and carry out the Yeast protein feed fermentation, reduce culture medium cost, improve saccharomycete bacterium number, for its application in aquaculture provides the basis.Yeast protein feed through the method fermenting and producing contains rich in protein, amino acid, vitamin and trace element, and contain digestive ferment and some unknown growth-stimulating factors, has higher biologically active, therefore, can promote animal metabolism, whet the appetite, be conducive to digesting and assimilating of animal, accelerate growing and the performance of production performance of animal, can also improve the defending and fighting against diseases ability of animal.Production technology simple and stable of the present invention, cell density height, tunning need not separation and purification and just can directly use, and are easy to promote; Yeast culture is added in the pig feed, all reach experimental result preferably, not only can prevent disease of digestive tract, and can promote poultry growth and put on weight, can be used as additive for farm animal feed.
The specific embodiment
Further specify the present invention by the following examples, but not as restriction of the present invention.
Embodiment 1 solid fermentation is produced the technological process of Yeast protein feed, may further comprise the steps:
(1) actication of culture:
The present invention adopt saccharomyces cerevisiae (
Saccharomyces cerevisiae) as fermented bacterium, saccharomyces cerevisiae claims Saccharomyces cerevisiae or budding yeast again, purchases in Guangdong Microbes Inst DSMZ preserving number: CICC1795.Saccharomyces cerevisiae is and mankind's relation primary yeast the most widely, it is used for making food and wine brewing such as bread and steamed bun traditionally, contain the good protein, the dietary fiber that meet the body demand, meet vitamin and organic selenium, elements such as chromium, zinc, iron, be the health-nutrition source of a kind of pure natural, pollution-free and environmental protection.Its protein content is higher, and amino acid balance is good, the digestibility height.The saccharomyces cerevisiae bacterial classification of-80 ℃ of preservations is rule at the YPD solid plate, cultivated 16 ~ 24 hours for 30 ℃;
(2) preparation first order seed:
With the saccharomyces cerevisiae bacterial classification of activation, be inoculated in the 200 mL YPD fluid nutrient mediums with oese picking one ring, triangular flask is placed shaking table, be that shaken cultivation made primary seed solution after 16 ~ 24 hours under 30 ℃ of conditions with 250rpm in temperature;
(3) preparation solid fermentation culture medium:
Solid butt: be that basestocks (contains corn flour 40 ~ 60wt%), glucose 2wt%, urea 0.5wt%, K with corn flour and wheat flour
2HPO
41.0wt% and biotin 0.01wt%.It is the sterilized water of 1:1 ~ 2 that the solid butt is added mass ratio, and is to stir under the natural conditions in pH value.Be under 121 ℃ with solid medium in temperature, autoclave sterilization 20min namely can be used as the solid fermentation culture medium after cooling and uses;
(4) solid fermentation: will insert the first order seed bacterium according to 5 ~ 10% inoculum concentration through the solid fermentation culture medium of sterilization treatment, and stir, and keep that humidity will be 50 ~ 70%, temperature is under 25 ~ 30 ℃ the condition, to cultivate about 20 hours, makes secondary seed.Secondary seed according to enlarging cultivation in 5 ~ 10% the inoculum concentration adding sterilization solid fermentation culture medium, is stirred, and keeping humidity is 50 ~ 70%, and temperature is 25 ~ 30 ℃ cultivated about 20 hours, made three grades of seeds.Can be according to concrete cultivation consumption, further three grades of seeds are further enlarged cultivation according to 5 ~ 10% inoculum concentration.Yeast culture is carried out colony counting, require viable count to reach more than 6.0 * 108cfu/g;
(5) the Yeast protein culture with solid fermentation carries out bake drying, pulverizes then and packs, and makes product.
Embodiment 2 Yeast protein feed products promote the application of growing-finishing pigs
Experimental group and control group are chosen 110 growing and fattening pigs of body weight about 68kg respectively, and experimental group 1.5% is added the Yeast protein feed product in feed, and control group does not add, and other experiment conditions are constant.
Experimental period is: 2012.04.22-----2012.05.25 day.Experimental result such as following table 1:
Table 1 Yeast protein feed product promotes the application experiment of growing-finishing pigs
Experimental result shows that the Yeast protein feed product can improve the growing and fattening pigs daily gain, improves efficiency of feed utilization, reduces feedstuff-meat ratio.
Embodiment 3 Yeast protein feed products promote the application of piglet growth
Select two identical booth piglets of wean age in days, be divided into two groups at random, 746 every group.First group is control group, does not add Yeast protein feed; Second group is test group, presses 2kg/t in the feed and adds Yeast protein feed than row.Test initial age in days and be 40 ages in days, weigh when 69 ages in days forward transformation fertilizer to, continue then to follow the tracks of, weigh again during sale.Feeding and management: free choice feeding, freely drink water, keep hurdle house cleaning.In off-test, choose 400 weanling pigs again and do repeated test.
Annotate: weaning weight is less than normal, is because the influence of diarrhoea causes.
Table 2 swinery basic condition
Table 3 feed consumption weightening finish table
Table 4 weightening finish and feedstuff-meat ratio
From table 3 and table 4 as can be seen, average daily feed intake of child care stage and every average daily weight increment test group of pig have not increased by 4.41% and 7.19% than the contrast component, and the feedstuff-meat ratio test group has then reduced by 5% than control group.
Table 5 fattening stage expenses for medicine and daily gain
As can be seen from Table 5, saved 42.22% in the fattening stage test group than average every the pig expenses for medicine of control group, daily gain has increased by 2.08%.
Contrast first group (control group) and second group of test group (the antibacterial peptide addition is 2kg/t), the average daily weightening finish of every pig of test group exceeds control group 29.5 g and 15.9g respectively in child care stage and fattening stage, improved 7.19% and 2.08% respectively, meanwhile, the feedstuff-meat ratio of test group is lower than control group, has reduced 10%(in the child care stage).
Claims (4)
1. the solid fermentation preparation method of a Yeast protein feed is characterized in that, may further comprise the steps:
(1) actication of culture: the fermented bacterium of-80 ℃ of preservations is rule at the YPD solid plate, cultivated 16 ~ 24 hours for 30 ℃;
(2) preparation first order seed: with the saccharomyces cerevisiae bacterial classification of activation, being inoculated in the 200 mL YPD fluid nutrient mediums with oese picking one ring, triangular flask is placed shaking table, is under the condition of 30 ℃ and 250rpm in temperature, after the shaken cultivation 16 ~ 24 hours, make the first order seed bacterium;
(3) preparation solid fermentation culture medium: it is the sterilized water of 1:1 ~ 2 that the solid butt is added mass ratio, and in the pH value for stirring under the natural conditions, under 121 ℃ condition, autoclave sterilization 20min namely can be used as the solid fermentation culture medium after cooling and uses with solid medium;
(4) solid fermentation: will insert the first order seed bacterium according to 5 ~ 10% inoculum concentration through the solid fermentation culture medium of sterilization treatment, and stir, and keep that humidity will be 50 ~ 70%, temperature is under 25 ~ 30 ℃ the condition, to cultivate about 20 hours, makes secondary seed;
(5) with the secondary seed that obtains, obtain three grades of seeds according to the further solid fermentation of step (4);
(6) can further three grades of seeds be enlarged according to the further solid fermentation of step (4) and cultivate according to concrete cultivation consumption.
2. the solid fermentation preparation method of Yeast protein feed according to claim 1, it is characterized in that: the solid butt in the described step (3) comprises: contain the corn flour of corn flour 40 ~ 60wt% and wheat flour as basestocks, glucose 2 wt %, urea 0.5 wt %, K
2HPO
41.0 wt % and biotin 0.01 wt %.
3. the solid fermentation preparation method of Yeast protein feed according to claim 1 and 2 is characterized in that: described fermented bacterium be saccharomyces cerevisiae (
Saccharomyces cerevisiae).
4. the solid fermentation preparation method of Yeast protein feed according to claim 3, it is characterized in that: described S. cervisiae is 0.5 ~ 2 wt % as the addition of feed addictive.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104206704A (en) * | 2014-05-27 | 2014-12-17 | 浙江科峰生物技术有限公司 | Preparation method of yeast culture |
| CN106190686A (en) * | 2015-05-07 | 2016-12-07 | 上海邦成生物工程有限公司 | A kind of solid fermentation prepares the method for saccharomyces cerevisiae |
| CN106551141A (en) * | 2016-11-18 | 2017-04-05 | 辽宁禾丰牧业股份有限公司 | A kind of young pig's mixed feed and its preparation method and application |
| CN106615706A (en) * | 2016-12-12 | 2017-05-10 | 安徽翠鸟生物技术有限公司 | Preparation method of non-active yeast powder |
Citations (1)
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| CN101756044A (en) * | 2008-10-21 | 2010-06-30 | 天津生机集团股份有限公司 | Method for producing fermented feed containing probiotics for pigs by using microbial fermentation |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101756044A (en) * | 2008-10-21 | 2010-06-30 | 天津生机集团股份有限公司 | Method for producing fermented feed containing probiotics for pigs by using microbial fermentation |
Non-Patent Citations (2)
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|---|
| 姚晓红等: "酵母蛋白饲料固体发酵培养基的筛选研究", 《浙江农业科学》, no. 02, 31 December 2007 (2007-12-31) * |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104206704A (en) * | 2014-05-27 | 2014-12-17 | 浙江科峰生物技术有限公司 | Preparation method of yeast culture |
| CN106190686A (en) * | 2015-05-07 | 2016-12-07 | 上海邦成生物工程有限公司 | A kind of solid fermentation prepares the method for saccharomyces cerevisiae |
| CN106551141A (en) * | 2016-11-18 | 2017-04-05 | 辽宁禾丰牧业股份有限公司 | A kind of young pig's mixed feed and its preparation method and application |
| CN106615706A (en) * | 2016-12-12 | 2017-05-10 | 安徽翠鸟生物技术有限公司 | Preparation method of non-active yeast powder |
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Application publication date: 20130911 |