CN107683972B - Improve the fermented feed and its preparation method and application of milking sow production performance - Google Patents
Improve the fermented feed and its preparation method and application of milking sow production performance Download PDFInfo
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- 238000004519 manufacturing process Methods 0.000 title claims abstract description 17
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 238000000855 fermentation Methods 0.000 claims abstract description 69
- 230000004151 fermentation Effects 0.000 claims abstract description 69
- 230000006651 lactation Effects 0.000 claims abstract description 24
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 20
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 20
- 240000008042 Zea mays Species 0.000 claims abstract description 20
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 20
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 18
- 235000005822 corn Nutrition 0.000 claims abstract description 18
- 230000004913 activation Effects 0.000 claims abstract description 17
- 239000007788 liquid Substances 0.000 claims abstract description 14
- 239000002994 raw material Substances 0.000 claims abstract description 12
- 239000000843 powder Substances 0.000 claims abstract description 10
- 238000006467 substitution reaction Methods 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 150000003839 salts Chemical class 0.000 claims abstract description 9
- 230000004099 anaerobic respiration Effects 0.000 claims abstract description 4
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 12
- 244000063299 Bacillus subtilis Species 0.000 claims description 11
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 11
- 241000894006 Bacteria Species 0.000 claims description 11
- 241000282898 Sus scrofa Species 0.000 claims description 10
- 235000012424 soybean oil Nutrition 0.000 claims description 10
- 239000003549 soybean oil Substances 0.000 claims description 10
- 235000005911 diet Nutrition 0.000 claims description 8
- 230000037213 diet Effects 0.000 claims description 8
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 7
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 7
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 7
- 235000019764 Soybean Meal Nutrition 0.000 claims description 6
- 239000004455 soybean meal Substances 0.000 claims description 6
- 241000196324 Embryophyta Species 0.000 claims description 4
- IJKRDVKGCQRKBI-UHFFFAOYSA-N Lactobacillinsaeure Natural products CCCCCCC1CC1CCCCCCCCCC(O)=O IJKRDVKGCQRKBI-UHFFFAOYSA-N 0.000 claims description 4
- 230000008901 benefit Effects 0.000 claims description 4
- IJKRDVKGCQRKBI-ZWKOTPCHSA-N lactobacillic acid Chemical compound CCCCCC[C@H]1C[C@H]1CCCCCCCCCC(O)=O IJKRDVKGCQRKBI-ZWKOTPCHSA-N 0.000 claims description 4
- 244000005700 microbiome Species 0.000 claims description 4
- 238000013019 agitation Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 2
- 235000013336 milk Nutrition 0.000 abstract description 12
- 239000008267 milk Substances 0.000 abstract description 12
- 210000004080 milk Anatomy 0.000 abstract description 12
- 206010012735 Diarrhoea Diseases 0.000 abstract description 6
- 235000021050 feed intake Nutrition 0.000 abstract description 6
- 235000019621 digestibility Nutrition 0.000 abstract description 5
- 235000016709 nutrition Nutrition 0.000 abstract description 5
- 235000015097 nutrients Nutrition 0.000 abstract description 3
- 230000035764 nutrition Effects 0.000 abstract description 3
- 230000033228 biological regulation Effects 0.000 abstract 1
- 239000013589 supplement Substances 0.000 abstract 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 12
- 239000000463 material Substances 0.000 description 12
- 230000006872 improvement Effects 0.000 description 6
- 239000004310 lactic acid Substances 0.000 description 6
- 235000014655 lactic acid Nutrition 0.000 description 6
- 235000019750 Crude protein Nutrition 0.000 description 5
- 235000019733 Fish meal Nutrition 0.000 description 5
- 244000068988 Glycine max Species 0.000 description 5
- 235000010469 Glycine max Nutrition 0.000 description 5
- 241000219823 Medicago Species 0.000 description 5
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 5
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 5
- 239000004467 fishmeal Substances 0.000 description 5
- 235000013312 flour Nutrition 0.000 description 5
- 239000004459 forage Substances 0.000 description 5
- 235000012054 meals Nutrition 0.000 description 5
- 239000006041 probiotic Substances 0.000 description 5
- 235000018291 probiotics Nutrition 0.000 description 5
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 230000000474 nursing effect Effects 0.000 description 4
- 229910052698 phosphorus Inorganic materials 0.000 description 4
- 239000011574 phosphorus Substances 0.000 description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 3
- 208000003643 Callosities Diseases 0.000 description 3
- 108010044091 Globulins Proteins 0.000 description 3
- 102000006395 Globulins Human genes 0.000 description 3
- 206010020649 Hyperkeratosis Diseases 0.000 description 3
- 230000000433 anti-nutritional effect Effects 0.000 description 3
- 238000009360 aquaculture Methods 0.000 description 3
- 244000144974 aquaculture Species 0.000 description 3
- 230000003115 biocidal effect Effects 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 229910052791 calcium Inorganic materials 0.000 description 3
- 235000019784 crude fat Nutrition 0.000 description 3
- 230000007812 deficiency Effects 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 108010083391 glycinin Proteins 0.000 description 3
- 229940099472 immunoglobulin a Drugs 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 2
- 238000007792 addition Methods 0.000 description 2
- 230000035606 childbirth Effects 0.000 description 2
- 235000021393 food security Nutrition 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 210000000936 intestine Anatomy 0.000 description 2
- 235000009973 maize Nutrition 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 230000035935 pregnancy Effects 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 235000005744 Bacillus subtilis subsp subtilis Nutrition 0.000 description 1
- 241000948854 Bacillus subtilis subsp. subtilis Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 244000164595 Lactobacillus plantarum subsp plantarum Species 0.000 description 1
- 235000012523 Lactobacillus plantarum subsp plantarum Nutrition 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 101710162629 Trypsin inhibitor Proteins 0.000 description 1
- 229940122618 Trypsin inhibitor Drugs 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 239000002956 ash Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000006027 corn-soybean meal Substances 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
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- 239000002753 trypsin inhibitor Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a kind of fermented feeds and its preparation method and application improving milking sow production performance.1）Prepare fermentation raw material；2）Activation medium is prepared, activation medium mass M is the 10%~15% of fermentation raw material gross mass, takes the bean cake powder of 2%~2.4%M that suitable quantity of water is added, filter to take clear liquid, the brown sugar of 1%~1.6%M is added, the salt of 0.1%~0.14%M is added, supplement water obtains activation medium；3）Activation fermentation seed liquid is simultaneously inoculated with；4）Pack fermentation, is fitted into anaerobic respiration bag, can be used when feed pH is down to 4.2 in bag.Fermentation mixed fodder carries out grade mass substitutions for improving milking sow production performance and Offspring Growth, by the corn in sow in lactation daily ration with the mixed fodder that ferments.The present invention can significantly increase milking sow feed intake and nutrient digestibility, increase IgA contents in the sow in lactation output of milk and milk, improve the wean weightening of piglet, reduce grice diarrhoea rate, the nutrition regulation under being cultivated for sow nonreactive provides a new approach.
Description
Technical field
The invention belongs to technical field of animal, and in particular to a kind of fermented feed and its preparation and be fed using its improvement
The method of newborn sow production performance and Offspring Growth performance.
Background technology
Food security and problems of ecological security were increasingly closed by society of various countries and government in recent years concerning the people's livelihood matter of fundamental importance
Note.One target of aquaculture is exactly the animal products such as the meat, eggs and milk of the high-quality green of production safety, is promoted the well-being of mankind.Feedstuff industry is made
For the upstream industry of aquaculture, play an important role in entire industrial chain.Antibiotic was pushed away in a very long time in past
The development of aquaculture has been moved, but abuse of antibiotics is reported again and again in recent years, remaining antibiotic passes through food chain to intelligent
Body may seriously damage the health of people, cause drug tolerant bacteria, while polluting environment.
Dam production performance is directly related to the economic benefit of farm, currently, China's sow productivity level is more low
Under, large-scale pig farm every sow in China's can provide weanling pig only about 15-19 heads every year, and the developed countries such as U.S. in 2008
Just have reached 20-24 heads.The principal element for causing China's sow productivity low has sow milk deficiency, feeding and management level low
Factors, the wherein hydropenic problems of sows in lactation milk such as lower and disease are increasingly paid attention to by everybody.It is intensive in China
Scale pig farm, sow in lactation feed intake is too low, and nutrition and Energy intaking are insufficient, causes sow milk deficiency, milk-quality not high
The case where take place frequently.Both of these problems seriously restrict the performance of China's sow productive potentialities.Dam nursing period does not control nutrition
Intake is easy to influence its lactation period output of milk and Offspring Growth performance.Therefore trophic factors, easy to digest is resisted in exploitation, and palatability is good
Dam feed rich in probiotic lactic acid and small peptide is of great significance.
Improve feed nutritive value by the means of microbial fermentation, produce green high-efficient feed have become at present it is foster
Grow the hot spot that industry is studied under " nonreactive " background.Fermentative feedstuff of microbe can not only provide beneficial bacterium and its metabolite, adjust
Gastrointestinal tract pH safeguards that intestinal flora balances, reduces the use of antibiotic, improves nutrient digestibility, moreover it is possible to develop and use new feeding
Expect resource, alleviates Food Security.The lactic acid that probiotics generates simultaneously can also inhibit the pathogenic bacterias such as Escherichia coli in feed
With the growth and breeding of pathogen, extend the feed holding time.Corn bean pulp type daily ration is the most widely used daily ration in China
Type, but due to the presence of the anti-nutritional factors such as antigen protein, trypsin inhibitor, bad oligosaccharides and cellulose, affect
Its application in livestock and poultry cultivation.Microbial fermentation corn bean pulp type mixed fodder can reduce anti-nutritional factors and improve its battalion
Support composition, but for fermented maize bean pulp type mixed fodder prepare research and on milking sow and improve sow productivity
Can it is extremely limited using upper report.
Invention content
For overcome the deficiencies in the prior art, the technical problem to be solved in the present invention is to provide a kind of lifes of improvement milking sow
Produce the fermented feed and its preparation method and application of performance.
A kind of fermented feed preparation method improving milking sow production performance, steps are as follows：
1）Prepare fermentation raw material
Take corn, dregs of beans and bran feedstock by quality 4:4:2~4.5:4.5:1 ratio premixing；
2）Prepare activation medium
Activation medium mass M is the 10%~15% of fermentation raw material gross mass, takes the bean cake powder of 2%~2.4%M to be added suitable
Water is measured, clear liquid is filtered to take, the brown sugar of 1%~1.6%M is added, the salt of 0.1%~0.14%M is added, benefit is filled with water to M and is activated
Then culture medium is divided into two parts；
3）Activation fermentation seed liquid is simultaneously inoculated with
Take 0.04%~0.07% lactobacillus plantarum ZJUAF-5 that fermentation raw material gross mass is added in a activation medium
Bacterium powder stands and is kept for 3~3.5 hours to get to plant lactobacillic acid bacterium fermentation seed liquid, takes in another activation medium and be added
The bacillus subtilis ZJUAF-3 bacterium powders of the 0.12%~0.15% of fermentation raw material gross mass, it is primary every agitation in 10~15 minutes,
Culture 3~3.5 hours is to get to fermentation of bacillus subtilis seed liquor；Above two fermentation seed liquid is mixed with water
Mixed activation fermentation seed liquid, access step 1）The fermentation raw material of preparation makes total moisture content account for the total matter of fermentation system
The 40%~45% of amount, stirs and evenly mixs；Above-mentioned lactobacillus plantarum (Lactobacillus plantarum subsp. plantarum
) buy in China Committee for Culture Collection of Microorganisms's common micro-organisms center, strain catalog number：1.510
, ZJUAF-5 is our name；Bacillus subtilis（Bacillus subtilis subsp.subtilis）ZJUAF-3 is also
For existing bacterial strain, it is originally presented in the CN106167782A of the applicant's application, China Committee for Culture Collection of Microorganisms
The preserving number of common micro-organisms center is CGMCC NO：12825.
4）Pack fermentation
Above-mentioned fermentation system is fitted into anaerobic respiration bag, 60% capacity is accounted for, is left to ferment 3 days, feed pH is down in bag
It can be used when 4.2.
The fermentation mixed fodder that a kind of fermented feed preparation method described in basis obtains.
A kind of application of fermentation mixed fodder described in basis, for improving milking sow production performance and Offspring Growth,
It is specific as follows：
Corn in sow in lactation daily ration is subjected to equal mass substitutions with the mixed fodder that ferments, replacement amount accounts for nursing period mother
The 7.5%~15% of swine rations gross weight, and the mass substitutions fermented bean dregs such as soybean oil for adding 1.5%~3%.
Preferably, with 13% corn in the 15% fermentation mass substitutions lactating swine diets such as+2% soybean oil of mixed fodder+
+ 1% citric acid of 3% dehulled soybean meal.
The fermentation mixed fodder nutritional ingredient：Crude protein >=28%, crude fat >=3.5%, crude fibre >=8%, small peptide
>=18%, pH≤4.3, β-companion's globulin content≤7mg/g, glycinin content≤8.5mg/g, lactic acid content >=
175mmol/Kg, bacillus subtilis viable count >=6.5 × 108, lactobacillus plantarum viable count >=4.5 × 108；Above-mentioned %
It is weight %.
The service stage of sow in lactation daily ration is：(3d after 3d- weaned piglets before childbirth).
The present invention is formulated with sow diets with reference to U.S. NRC（2012）Standard, according to energy, albumen phase approximately principle；Fermentation
Feed is directly mixed by adding proportion with milking sow basestocks behind Kaifeng, is fed in the form of wet-mixing material, and feed granulating etc. is reduced
Inactivation of the process to probiotics.
Compared with prior art, the present invention has the advantages that：
The present invention can significantly increase milking sow feed intake and nutrient digestibility, increase the sow in lactation output of milk and
IgA contents in milk improve the wean weightening of nursery-age pig, reduce grice diarrhoea rate.
Description of the drawings
Fig. 1 is the fermentation mixed fodder prepared by the embodiment of the present invention 1；
Fig. 2 is control group and fermentation group mother, piglet in embodiment 4.
Specific implementation mode
Below in conjunction with drawings and examples, the present invention is further elaborated.
A kind of preparation method of 1 fermented maize dregs of beans mixed fodder of embodiment
Take the bean cake powder of 1Kg that 90 DEG C or so of 100Kg clear water is added, 8 layers of hospital gauze filter or take supernatant liquor, filtrate
The middle brown sugar and 100g salt that 500g is added, is then divided into two parts of additions.It takes a culture solution to cool to 37 DEG C, is added
The plant lactobacillic acid bacterium powder of 240g.Closed standing keep 3 hours or so to get to plant lactobacillic acid bacterium fermentation seed liquid.It takes another
A culture solution cools to 37 DEG C, and the bacillus subtilis powder of 700g is added.It is primary every agitation in 15 minutes, it cultivates 3 hours
Later to get to fermentation of bacillus subtilis seed liquor.200Kg clear water by above-mentioned activated bacterium solution with 40 DEG C in bucket mixes
It closes uniform.280Kg corns, 280Kg dregs of beans and 140Kg bran feedstocks are taken, the fermentation seed liquid of above-mentioned preparation is accessed into compound feeding
In material, blender stirs 5 minutes mixings.Above-mentioned material is fitted by the amount of mono- bag of 20Kg in the anaerobic respiration bag for 35Kg, is detested
Oxygen canister is equipped with unidirectional venthole, and gas can only be discharged out of bag, is left to ferment 3 days or so after plastic packaging, and feed PH will in bag
It can be used to when 4.2 or so, as shown in Figure 1.
The front and back nutritional ingredient variation of 1. corn-soybean meal mixed fodder of table fermentation
| Project | Do not ferment mixed fodder | Ferment mixed fodder |
| Dry matter, % | 91.21 | 90.07 |
| Crude protein, % | 25.79 | 28.16 |
| The molten albumen of acid, % | 4.58 | 18.14 |
| Crude fat, % | 3.67 | 3.37 |
| Coarse ash, % | 3.99 | 4.56 |
| Calcium, % | 0.18 | 0.20 |
| Total phosphorus, % | 0.47 | 0.53 |
| Β is with globulin, mg/g | 31.93 | 7.02 |
| Glycinin, mg/g | 63.68 | 8.46 |
| pH | 6.55 | 4.02 |
| Lactic acid content, mmol/Kg | - | 174.57 |
| Bacillus subtilis, cfu/g | - | 6.4×108 |
| Lactobacillus plantarum, cfu/g | - | 4.6×108 |
As shown in Table 1, compared with the mixed fodder that do not ferment, mixed fodder crude protein, coarse ash, calcium and phosphorus difference after fermentation
2.37%, 13.56%, 0.57%, 0.02% and 0.06% is improved, β drops with globulin and glycinin in mixed fodder after fermentation
Solution is apparent, degrades 24.91%, 55.22% respectively, and lactic acid content runs up to 174.57mmol/Kg after fermentation 3 days, feed pH by
Gradually it is reduced to 4.02, the quantity of bacillus subtilis and lactobacillus plantarum respectively reaches 6.4 × 108With 4.6 × 108, probiotics contains
Amount is abundant.To sum up, obtained fermentation mixed fodder anti-nutritional factors is low, lactic acid and probiotics rich content.
As shown in Fig. 2, being fermentation mixed fodder in bag, quality is good.
Embodiment 2 is a kind of to be sent out with 7.5% corns+1.5% of mass substitutions such as 7.5% fermentation+1.5% soybean oils of mixed fodder
Ferment dregs of beans makes lactation sow forage
The formula of sow in lactation daily ration after present invention improvement is：
7.5% fermentation mixed fodder, 52.5% corn, 8% dehulled soybean meal, 11% expanded soybean, 3.5%
Fermented bean dregs, 3% alfalfa meal, 3% fish meal, 1.5% soybean oil, 3.8% yeast hydrolyate, 0.2% soda, 0.4% food
Salt, 0.6% mountain flour and 5% premix, are put into blender, are sufficiently stirred to get being 7.5% to fermentation mixed fodder content
Lactation sow forage.
Embodiment 3 is a kind of with+3% fermented bean dregs of 15% corn of mass substitutions such as 15% fermentation+3% soybean oils of mixed fodder
Make lactation sow forage
The formula of sow in lactation daily ration after present invention improvement is：
15% fermentation mixed fodder, 45.0% corn, 8.0% dehulled soybean meal, 11% expanded soybean, 2% hair
Ferment dregs of beans, 3% alfalfa meal, 3% fish meal, 3.0% soybean oil, 3.8% yeast hydrolyate, 0.2% soda, 0.4% salt,
0.6% mountain flour and 5% premix, are put into blender, are sufficiently stirred to get the food in one's mouth for being 15% to fermentation mixed fodder content
Newborn phase sow feed.
Embodiment 4 is another to remove the peel beans with 13% corns+3% of mass substitutions such as 15% fermentation+2% soybean oils of mixed fodder
+ 1% citric acid of the dregs of rice makes lactation sow forage
The formula of sow in lactation daily ration after present invention improvement is：
15% fermentation mixed fodder, 52% corn, 6% dehulled soybean meal, 14% expanded soybean, 2% alfalfa meal,
3% fish meal, 2% soybean oil, 1.0% yeast hydrolyate, 0.1% soda, 0.4% salt, 0.5% mountain flour and 4% it is pre-
Batch mixing is put into blender, is sufficiently stirred to get the lactation sow forage for being 15% to fermentation mixed fodder content.
1 sow in lactation application test of application test
The ratio of control group auxiliary material each component is：60% corn, 8.0% dregs of beans, 11% expanded soybean, 5%
Fermented bean dregs, 3% alfalfa meal, 3% fish meal, 3.8% yeast hydrolyate, 0.2% soda, 0.4% salt, 0.6% mountain flour
Premix with 5%, is put into blender, is sufficiently stirred.Control group, embodiment 2, embodiment 3 respectively take 20 long white binary sow
It is tested.To taking care of, the feeding period of 3 groups of feeds of embodiment 2 and embodiment 3 be：Antenatal 3 days (gestation the 111st of sow
It) is to 22 days or so weaned piglets after Farrowing.
Influence of the sow diets that 2. embodiment of the present invention 2,3 of table and comparative example provide to sow production performance
| Project | Control group | 7.5% fermentation material group | 15% fermentation material group |
| Sow in lactation feed intake, Kg/ days | 5.50b | 5.93ab | 6.62a |
| It loses flesh situation, mm | 3.00a | 1.25b | 1.42b |
Note：Shoulder mark difference of going together lowercase letter indication difference significantly (P<0 .05) as shown in Table 2, it is compared with the control group, real
0.43kg/d (P are respectively increased in the food intake of sows in lactation for applying example 2 and embodiment 3>0 .05) and 1.12kg/d (P<0 .05),
Sows in lactation situation of losing flesh reduces 1.75mm (P respectively>0 .05) and 1.58mm (P>0 .05).
Influence of the sow diets that 3. embodiment of the present invention 2,3 of table and comparative example provide to piglet growth performance
| Project | Control group | 7.5% fermentation material group | 15% fermentation material group |
| Weigth at birth, Kg | 18.75 | 17.99 | 18.65 |
| Number born alive | 14.75 | 14.38 | 14.00 |
| Weight of weaning litter, Kg | 70.35 | 71.99 | 73.21 |
| Wean survival rate, % | 93.60 | 94.50 | 96.33 |
| The nest that weans increases weight, Kg | 53.63 | 54.01 | 58.63 |
| Piglet Birth weight, Kg | 1.27 | 1.26 | 1.34 |
| Weaned piglet weight, Kg | 5.93b | 5.98b | 6.45a |
| Piglet increases weight, Kg | 4.52b | 4.64b | 5.13a |
| Diarrhea rate, % | 2.11 | 1.95 | 1.83 |
Note：Shoulder mark difference of going together lowercase letter indication difference significantly (P<0 .05)
As shown in Table 3, compared with the control group, 0.52Kg (P are respectively increased in the weaned piglet of embodiment 3 and embodiment 2 again<0
.05)、O.05Kg(P>0 .05), 0.61Kg (P are respectively increased in weaned piglet weightening<0 .05)、0.12Kg(P>0 .05).Other
Index there are no significant variation.
According to above-mentioned table 2, table 3, we it can be seen that：Fermentation mixed fodder can improve sow production performance, promote piglet
Growth performance, and the overall effect of embodiment 3 is better than embodiment 2.
2 sow in lactation another application of application test is tested
The ratio of another control group nursing period auxiliary material each component is：65% corn, 9% dehulled soybean meal, 14% it is swollen
Change soybean, 2% alfalfa meal, 3% fish meal, 1.0% yeast hydrolyate, 1% citric acid, 0.1% soda, 0.4% salt,
0.5% mountain flour and 4% premix, are put into blender, are sufficiently stirred.Control group and embodiment 4 respectively take 30 long white binary female
Pig is tested.To taking care of and the feeding period of 2 groups of feeds of embodiment 4 is：Sow antenatal 3 days (gestation the 111st day) is extremely female
22 days or so weaned piglets after pig childbirth.
Influence of the sow diets that 4. embodiment of the present invention 4 of table and comparative example provide to sow production performance
| Project | Control group | 15% fermentation material group |
| Sow in lactation feed intake, Kg/ days | 4.66b | 5.50a |
| It loses flesh situation, mm | 2.41a | 1.36b |
| Wean inter oestrual period, day | 7.54 | 5.36 |
| Milk production, Kg/ days | 8.57b | 9.81a |
| Butterfat | 7.48 | 7.76 |
| Lactose | 5.71 | 5.87 |
| Lactoprotein | 4.79 | 4.80 |
| Newborn immunoglobulin A, mg/ml | 4.35b | 5.72a |
Note：Shoulder mark difference of going together lowercase letter indication difference significantly (P<0 .05)
As shown in Table 4, compared with the control group, 4 sow average daily gain of embodiment significantly improves 0.84Kg (P<0
.05), nursing period, which loses flesh, is substantially reduced 1.05mm (P<0 .05), wean inter oestrual period shortens 2.18 days (P>0 .05), sow production
Milk amount and immunoglobulin A content significantly improve 1.24Kg, 1.37mg/ml.Milk-quality is without significant difference.
Influence of the sow diets that 5. embodiment of the present invention 4 of table and comparative example provide to piglet growth performance
| Project | Control group | 15% fermentation material group |
| Weigth at birth, Kg | 14.41 | 13.69 |
| Number born alive | 9.45 | 10.64 |
| Weight of weaning litter, Kg | 52.15 | 60.11 |
| Wean survival rate, % | 94.70 | 97.01 |
| The nest that weans increases weight, Kg | 35.45b | 44.06a |
| Piglet Birth weight, Kg | 1.45 | 1.40 |
| Weaned piglet weight, Kg | 5.63 | 6.33 |
| Piglet increases weight, Kg | 3.92b | 4.73a |
| Diarrhea rate, % | 5.25a | 2.98b |
Note：Shoulder mark difference of going together lowercase letter indication difference significantly (P<0 .05)
As shown in Table 5, compared with the control group, the piglet nest wean weightening of embodiment 4 improves 8.61Kg (P<0 .05), it is young
0.70Kg (P are respectively increased in pig weaning weight and wean weightening>0 .05)、O.81Kg (P<0 .05), grice diarrhoea rate significantly reduces
2.27% (P<0 .05).Other indexs there are no significant variation.As shown in Fig. 2, sow production performance and the piglet of embodiment 4
Growth performance has improvement.
Influence of the sow diets that 6. embodiment of the present invention 4 of table and comparative example provide to the full intestines apparent digestibility of sow
| Project | Control group | 15% fermentation material group |
| Total energy | 82.65b | 83.92a |
| Dry matter | 83.13b | 84.18a |
| Crude protein | 84.53b | 86.29a |
| Crude fat | 58.73 | 63.92 |
| Coarse ash | 37.20 | 40.82 |
| Calcium | 40.93 | 48.06 |
| Total phosphorus | 36.10b | 41.99a |
Note：Shoulder mark difference of going together lowercase letter indication difference significantly (P<0 .05)
As shown in Table 6, compared with the control group, the full intestines table of the sow total energy of embodiment 4, dry matter, crude protein and total phosphorus
It sees digestibility to be significantly increased, 1.27% (P is respectively increased<0 .05)、1.05%(P<0 .05)、1.76%(P<0 .05) and
5.89%(P<0 .05)。
In conclusion we it can be seen that：The addition of 15% fermentation mixed fodder can be by improving sow feed intake and digestion
Rate improves the content of newborn immunoglobulin A to improve milk production, and then piglet is promoted to grow and reduce diarrhea.
Claims (4)
1. a kind of fermented feed preparation method improving milking sow production performance, which is characterized in that steps are as follows：
1）Prepare fermentation raw material
Take corn, dregs of beans and bran feedstock by quality 4:4:2~4.5:4.5:1 ratio premixing；
2）Prepare activation medium
Activation medium mass M is the 10%~15% of fermentation raw material gross mass, takes the bean cake powder of 2%~2.4%M that suitable quantity of water is added,
Clear liquid is filtered to take, the brown sugar of 1%~1.6%M is added, the salt of 0.1%~0.14%M is added, benefit is filled with water to M and obtains activation culture
Then base is divided into two parts；
3）Activation fermentation seed liquid is simultaneously inoculated with
Take 0.04%~0.07% lactobacillus plantarum ZJUAF-5 bacterium that fermentation raw material gross mass is added in a activation medium
Powder stands and is kept for 3~3.5 hours to get addition hair in another activation medium to plant lactobacillic acid bacterium fermentation seed liquid, is taken
The bacillus subtilis ZJUAF-3 bacterium powders of the 0.12%~0.15% of ferment raw material gross mass, training primary every agitation in 10~15 minutes
3~3.5 hours are supported to get to fermentation of bacillus subtilis seed liquor；Above two fermentation seed liquid is mixed with water
To mixed activation fermentation seed liquid, step 1 is accessed）The fermentation raw material of preparation makes total moisture content account for fermentation system gross mass
40%~45%, stir and evenly mix；The lactobacillus plantarum ZJUAF-5 is bought in China Committee for Culture Collection of Microorganisms
Common micro-organisms center, strain catalog number：1.510 ；The bacillus subtilis ZJUAF-3, Chinese microorganism strain are protected
The preserving number for hiding administration committee's common micro-organisms center is CGMCC NO：12825；
4）Pack fermentation
Above-mentioned fermentation system is fitted into anaerobic respiration bag, 60% capacity is accounted for, is left to ferment 3 days, feed pH is down to 4.2 in bag
When can be used.
2. a kind of fermentation mixed fodder that fermented feed preparation method according to claim 1 obtains.
3. a kind of application of fermentation mixed fodder according to claim 2, which is characterized in that for improving milking sow life
Performance and Offspring Growth are produced, it is specific as follows：
Corn in sow in lactation daily ration is subjected to equal mass substitutions with the mixed fodder that ferments, replacement amount accounts for sow in lactation day
The 7.5%~15% of grain gross weight, and the mass substitutions fermented bean dregs such as soybean oil for adding 1.5%~3%.
4. application according to claim 3, which is characterized in that it is preferred, with 15% fermentation+2% soybean oil of mixed fodder etc.
+ 1% citric acid of+3% dehulled soybean meal of 13% corn in mass substitution lactating swine diets.
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| CN109770047A (en) * | 2019-03-15 | 2019-05-21 | 刘坚 | A kind of sows in lactation feed and preparation method thereof |
| CN109953202A (en) * | 2019-03-15 | 2019-07-02 | 河南龙凤山农牧股份有限公司 | A kind of antibiotic-free pannage |
| CN110403060A (en) * | 2019-03-22 | 2019-11-05 | 河南龙凤山农牧股份有限公司 | A kind of antibiotic-free grower pigs fermented feed and preparation method thereof |
| CN111887342A (en) * | 2020-07-10 | 2020-11-06 | 徐州生物工程职业技术学院 | Compound lactobacillus fermented feed and preparation and feeding method thereof |
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