CN102293346B - Fermented feed for piglets and preparation method thereof - Google Patents
Fermented feed for piglets and preparation method thereof Download PDFInfo
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- CN102293346B CN102293346B CN2011102595465A CN201110259546A CN102293346B CN 102293346 B CN102293346 B CN 102293346B CN 2011102595465 A CN2011102595465 A CN 2011102595465A CN 201110259546 A CN201110259546 A CN 201110259546A CN 102293346 B CN102293346 B CN 102293346B
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- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 241000894006 Bacteria Species 0.000 claims abstract description 27
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 18
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 17
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 17
- 240000008042 Zea mays Species 0.000 claims abstract description 17
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 17
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 17
- 235000005822 corn Nutrition 0.000 claims abstract description 17
- 239000002994 raw material Substances 0.000 claims abstract description 15
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 11
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 11
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 8
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 8
- 235000013557 nattō Nutrition 0.000 claims abstract description 8
- 239000000725 suspension Substances 0.000 claims description 33
- 241000235342 Saccharomycetes Species 0.000 claims description 16
- 235000015099 wheat brans Nutrition 0.000 claims description 16
- 235000013312 flour Nutrition 0.000 claims description 15
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 11
- 230000004913 activation Effects 0.000 claims description 6
- 238000009630 liquid culture Methods 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 238000011081 inoculation Methods 0.000 claims description 4
- 206010012735 Diarrhoea Diseases 0.000 abstract description 10
- 238000004519 manufacturing process Methods 0.000 abstract description 8
- 230000036039 immunity Effects 0.000 abstract description 6
- 239000004382 Amylase Substances 0.000 abstract description 5
- 102000013142 Amylases Human genes 0.000 abstract description 5
- 108010065511 Amylases Proteins 0.000 abstract description 5
- 108091005804 Peptidases Proteins 0.000 abstract description 5
- 239000004365 Protease Substances 0.000 abstract description 5
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract description 5
- 235000019418 amylase Nutrition 0.000 abstract description 5
- 150000004676 glycans Chemical class 0.000 abstract description 5
- 229920001282 polysaccharide Polymers 0.000 abstract description 5
- 239000005017 polysaccharide Substances 0.000 abstract description 5
- 238000000034 method Methods 0.000 abstract description 4
- 229920001184 polypeptide Polymers 0.000 abstract description 4
- 102000004196 processed proteins & peptides Human genes 0.000 abstract description 4
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 4
- 239000000654 additive Substances 0.000 abstract description 2
- 239000007787 solid Substances 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 208000035240 Disease Resistance Diseases 0.000 abstract 1
- 239000003963 antioxidant agent Substances 0.000 abstract 1
- 238000009395 breeding Methods 0.000 abstract 1
- 230000001488 breeding effect Effects 0.000 abstract 1
- 230000000968 intestinal effect Effects 0.000 abstract 1
- 235000012054 meals Nutrition 0.000 abstract 1
- 230000000813 microbial effect Effects 0.000 abstract 1
- 239000006041 probiotic Substances 0.000 abstract 1
- 230000000529 probiotic effect Effects 0.000 abstract 1
- 235000018291 probiotics Nutrition 0.000 abstract 1
- 239000000463 material Substances 0.000 description 11
- 238000012360 testing method Methods 0.000 description 8
- 239000012467 final product Substances 0.000 description 5
- 244000005700 microbiome Species 0.000 description 5
- 230000028327 secretion Effects 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000010171 animal model Methods 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 230000001079 digestive effect Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 102000040350 B family Human genes 0.000 description 1
- 108091072128 B family Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 230000025507 digestive system development Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a fermented feed for piglets and a preparation method thereof and belongs to the technical field of feed production. The feed comprises Bacillus subtilis natto, Saccaeomrces. cerevisiae and Lactobacillus plantarum, and is prepared by fermenting corn meal, bean pulp and bran which are taken as raw materials by a solid culture technology. The produced feed contains a large number of probiotic functional bacteria, and is also rich in substances such as polypeptide, polysaccharide, protease, amylase, microbial antioxidants and the like. The feed can improve the balance of intestinal floras, and remarkably prevent and treat the diarrhea of the piglets; meanwhile, the feed can be well absorbed, can improve the immunity, strengthen disease resistance and greatly improve the breeding income of farmers, is a safe and efficient novel feed, and is suitable for industrialized production, the daily gain of the piglets is quick, and additives are not required to be added in the feeding process.
Description
Technical field
The present invention relates to the Feed Production Technology field, particularly a kind of fermented feed for piglets and preparation method thereof.
Background technology
The annual production of China's feed industry is only second to the U.S. and is positioned at the second place of the world, and the output value also occupy the 20th in 41 main industries of Chinese national economy, has become one of basic industry of national economy.Piglet is the fastest period of growing, and the body weight in 6 weeks is connatae 10 times after the birth, especially, newborn piglet is because digestive system development is not perfect, and a little less than the secretion digestive ferment ability, immunity is also relatively poor, if the Direct-fed complete feed often causes trophism diarrhea, in addition dead.Ministry of Agriculture's statistical report demonstration in the end of the year 2008, the death rate of the onset of grice diarrhoea causes approximately more than 300 hundred million yuan loss up to about 35% every year in China.So, be starved of the feed that is different from common complete diet pellet, yet, up to the present, also do not have a kind of piglet special feed can utilize benefit to give birth to the materials such as the polysaccharide of function bacterium and secretion thereof, protease, amylase, microorganism polyphenoils, reach the digestic property that improves piglet, the purpose that reduces rate of death caused by diarrhoea.
Summary of the invention
The object of the present invention is to provide a kind of fermented feed for piglets and preparation method thereof, solved the problems referred to above that present technology exists.The present invention is take corn flour, dregs of beans and wheat bran as raw material, ferment by beneficial microorganism, the fermented feed for piglets of the materials such as beneficial microbe, polypeptide, polysaccharide, protease, amylase, microorganism polyphenoils is rich in acquisition, thereby make piglet obtain more fully nutrition, strengthen immunity, significantly promote weightening finish, improve raiser's economic benefit.The present invention utilizes bafillus natto, saccharomycete, the Lactobacillus plantarum combined ferment is produced Novel piglet feed and method, this novel fodder is with corn flour, dregs of beans, wheat bran is raw material, adopt the fermentation of solid culture technology, the feed of producing not only contains a large amount of benefits and gives birth to function bacterium, and, also be rich in polypeptide, polysaccharide, protease, amylase, the materials such as microorganism polyphenoils, the characteristics of this feed are to improve the balance of gut flora, significantly prevent and the treatment grice diarrhoea, simultaneously, good absorbing, daily gain is fast, immunity improves, resistance against diseases strengthens, and the peasant cultivates income and will increase substantially, and is a kind of safety, efficient novel fodder, in the process of feeding, need not add any additive, and be suitable for suitability for industrialized production.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
Fermented feed for piglets, made by the raw material of following portions by weight ratio:
The plastc ring of 40 ~ 60 parts of corn flour, 20 ~ 40 parts of dregs of beans, 10 ~ 20 parts in wheat bran, 0.8 ~ 1.2 times of above-mentioned raw materials parts by weight; Described plastc ring is that the by volume ratio mixing of 4:2:3 of bafillus natto bacteria suspension, saccharomycete bacteria suspension and Lactobacillus plantarum bacteria suspension makes.
Fermented feed for piglets, made by the raw material of following portions by weight ratio:
The plastc ring of 41 ~ 48 parts of corn flour, 32 ~ 39 parts of dregs of beans, 13 ~ 19 parts in wheat bran, 0.8 ~ 1.2 times of above-mentioned raw materials parts by weight; Described plastc ring is that the by volume ratio mixing of 4:2:3 of bafillus natto bacteria suspension, saccharomycete bacteria suspension and Lactobacillus plantarum bacteria suspension makes.
Another object of the present invention is to provide a kind of preparation method of above-mentioned fermented feed for piglets, comprise the steps:
To cross 10 ~ 30 mesh sieves after above-mentioned corn flour, dregs of beans, the wheat bran mixing, and access above-mentioned plastc ring, connecing the bacterium amount is 1.0 * 10
6Individual/the g material, after cultivating 60 ~ 120h under 25 ~ 40 ℃ of conditions, pulverize and get final product.
In the present invention, beneficial microbe bafillus natto, saccharomycete and Lactobacillus plantarum are conducive to improve the microecological balance of intestine of young pigs, improve the production performance of piglet.Reduce diarrhea rate, improve the cellular immune level of piglet.And, a large amount of protease of bafillus natto, Lactobacillus plantarum secretion, amylase etc., remedy on the one hand the deficiency of piglet autodigestion enzyme secretion, can be the material such as polypeptide, polysaccharide of absorption more easy to digest on the other hand with substance decomposition such as the carbohydrate in corn, dregs of beans and the wheat bran, protein, improve its utilization rate.Simultaneously, also be rich in microorganism polyphenoils, trace element, vitamin E and B family vitamin etc. in the fermented feed, can significantly promote healthy growth and the growth of piglet.Improve immunity, reduce rate of death caused by diarrhoea.
Distinguishing feature of the present invention is: this fermented feed for piglets nutrition is complete, need not to add any feed addictive during use, residual without harmful components after the piglet metabolism, can significantly improve abilities of digestive and absorption and the immunity of piglet, greatly reduce its rate of death caused by diarrhoea, have significant economic benefit.
The specific embodiment:
The below further specifies detailed content of the present invention and the specific embodiment thereof.
Embodiment 1:The preparation of bacteria suspension
The preparation of bafillus natto bacteria suspension
1) the dull and stereotyped cultivation activates: bafillus natto is inoculated in the dull and stereotyped cultivation of LB, cultivates 14 ~ 30h under 35 ~ 40 ℃ of conditions, form single bacterium colony;
2) with the bafillus natto bacterial classification inoculation after the step 1) activation culture in the LB Liquid Culture, under 35 ~ 40 ℃, 180 ~ 240rpm condition, cultivate 8 ~ 16h, make it be in the logarithm middle and later periods;
3) with step 2) thalline that obtains centrifugal collection thalline under 4 ℃, 4000rpm condition, be diluted to 1.0 ~ 2.0 * 10 with SPSS
11Individual/ml is for subsequent use.
The preparation of saccharomycete bacteria suspension
1) the dull and stereotyped cultivation activates: saccharomycete is inoculated in the dull and stereotyped cultivation of PDA, cultivates 20 ~ 36h under 25 ~ 35 ℃ of conditions, form single bacterium colony;
2) saccharomycete with the step 1) activation culture is inoculated in the PDA Liquid Culture, cultivates 12 ~ 24h under 25 ~ 35 ℃, 200 ~ 240rpm condition, makes it be in the logarithm middle and later periods;
3) with step 2) thalline that obtains centrifugal collection thalline under 4 ℃, 4000rpm condition, be diluted to 1.0 ~ 2.0 * 10 with SPSS
11Individual/ml is for subsequent use.
The preparation of Lactobacillus plantarum bacteria suspension
1) the dull and stereotyped cultivation activates: Lactobacillus plantarum is inoculated in the dull and stereotyped cultivation of MRS, cultivates 24 ~ 36h under 30 ~ 40 ℃ of conditions, form single bacterium colony;
2) with the Lactobacillus plantarum bacterial classification inoculation of step 1) activation culture in the MRS Liquid Culture, under 30 ~ 40 ℃ of conditions, cultivate 12 ~ 24h, make it be in the logarithm middle and later periods;
3) with step 2) thalline that obtains centrifugal collection thalline under 4 ℃, 4000rpm condition, be diluted to 1.0-2.0 * 10 with SPSS
11Individual/ml is for subsequent use.
The by volume ratio mixing of 4:2:3 of above-mentioned three kinds of bacteria suspensions is obtained plastc ring.
Embodiment 2:
Fermented feed for piglets, made by the raw material of following portions by weight ratio:
50 parts of corn flour, 30 parts of dregs of beans, 20 parts in wheat bran, 80 parts of plastc rings; Described plastc ring is that the by volume ratio mixing of 4:2:3 of bafillus natto bacteria suspension, saccharomycete bacteria suspension and Lactobacillus plantarum bacteria suspension makes.
The preparation method of above-mentioned fermented feed for piglets is: cross 10 ~ 30 mesh sieves after above-mentioned corn flour, dregs of beans, wheat bran are mixed, and access above-mentioned plastc ring, connecing the bacterium amount is 1.0 * 10
6Individual/the g material, after cultivating 72h under 30 ℃ of conditions, pulverize and get final product.
Embodiment 3:
Fermented feed for piglets, made by the raw material of following portions by weight ratio:
60 parts of corn flour, 30 parts of dregs of beans, 10 parts in wheat bran, 100 parts of plastc rings; Described plastc ring is that the by volume ratio mixing of 4:2:3 of bafillus natto bacteria suspension, saccharomycete bacteria suspension and Lactobacillus plantarum bacteria suspension makes.
The preparation method of above-mentioned fermented feed for piglets is: cross 10 ~ 30 mesh sieves after above-mentioned corn flour, dregs of beans, wheat bran are mixed, and access above-mentioned plastc ring, connecing the bacterium amount is 1.0 * 10
6Individual/the g material, under 25 ℃ of conditions, cultivate 120h and pulverize and get final product.
Embodiment 4:
Fermented feed for piglets, made by the raw material of following portions by weight ratio:
60 parts of corn flour, 20 parts of dregs of beans, 20 parts in wheat bran, 120 parts of plastc rings; Described plastc ring is that the by volume ratio mixing of 4:2:3 of bafillus natto bacteria suspension, saccharomycete bacteria suspension and Lactobacillus plantarum bacteria suspension makes.
The preparation method of above-mentioned fermented feed for piglets is: cross 10 ~ 30 mesh sieves after above-mentioned corn flour, dregs of beans, wheat bran are mixed, and access above-mentioned plastc ring, connecing the bacterium amount is 1.0 * 10
6Individual/the g material, under 35 ℃ of conditions, cultivate 100h and pulverize and get final product.
Embodiment 5:
Fermented feed for piglets, made by the raw material of following portions by weight ratio:
45 parts of corn flour, 39 parts of dregs of beans, 16 parts in wheat bran, 110 parts of plastc rings; Described plastc ring is that the by volume ratio mixing of 4:2:3 of bafillus natto bacteria suspension, saccharomycete bacteria suspension and Lactobacillus plantarum bacteria suspension makes.
The preparation method of above-mentioned fermented feed for piglets is: cross 10 ~ 30 mesh sieves after above-mentioned corn flour, dregs of beans, wheat bran are mixed, and access above-mentioned plastc ring, connecing the bacterium amount is 1.0 * 10
6Individual/the g material, under 37 ℃ of conditions, cultivate 80h and pulverize and get final product.
Embodiment 6:Test
Experimental animal: select 21 ages in days wean, as experimental animal, totally 60, a collection of choosing is neat at the Chang-Bai piglet of 8.5 ~ 9.5kg for body weight, and the date of birth differs and is no more than 5 days.
Experimental design: two groups of test minute control group and experimental group, every group of triplicate, each 10 piglets are between group and repeat different 5% of the average weight that is no more than of the mesosome method of double differences.Control group fed complete feed, the test group fermented feed for piglets of the present invention of feeding, 30d continuously feeds.
Method of testing and index: weigh to piglet morning on an empty stomach when on-test and end, calculates the indexs such as equal daily gain, material anharmonic ratio and diarrhea rate.
Result of the test: the production performance of test piglet sees Table 1
The production performance of table 1 test piglet
By as seen from Table 1, test group improves 12.6% than control group aspect equal daily gains, and the material anharmonic ratio reduces by 8.6%, and diarrhea rate has descended 72.2%.
In addition, can adjust the proportioning mode of above feed according to the concrete condition of piglet, mix the piglet optimum feed that is fit to different situations.
Claims (2)
1. fermented feed for piglets, it is characterized in that: the raw material by the following portions by weight ratio is made:
The plastc ring of 40 ~ 60 parts of corn flour, 20 ~ 40 parts of dregs of beans, 10 ~ 20 parts in wheat bran, 0.8 ~ 1.2 times of above-mentioned raw materials parts by weight; Described plastc ring is that the by volume ratio mixing of 4:2:3 of bafillus natto bacteria suspension, saccharomycete bacteria suspension and Lactobacillus plantarum bacteria suspension makes;
The preparation of described bafillus natto bacteria suspension is:
1) the dull and stereotyped cultivation activates: bafillus natto is inoculated in the dull and stereotyped cultivation of LB, cultivates 14 ~ 30h under 35 ~ 40 ℃ of conditions, form single bacterium colony;
2) with the bafillus natto bacterial classification inoculation after the step 1) activation culture in the LB Liquid Culture, under 35 ~ 40 ℃, 180 ~ 240rpm condition, cultivate 8 ~ 16h, make it be in the logarithm middle and later periods;
3) with step 2) thalline that obtains centrifugal collection thalline under 4 ℃, 4000rpm condition, be diluted to 1.0 ~ 2.0 * 10 with SPSS
11Individual/ml is for subsequent use;
The preparation of described saccharomycete bacteria suspension is:
1) the dull and stereotyped cultivation activates: saccharomycete is inoculated in the dull and stereotyped cultivation of PDA, cultivates 20 ~ 36h under 25 ~ 35 ℃ of conditions, form single bacterium colony;
2) saccharomycete with the step 1) activation culture is inoculated in the PDA Liquid Culture, cultivates 12 ~ 24h under 25 ~ 35 ℃, 200 ~ 240rpm condition, makes it be in the logarithm middle and later periods;
3) with step 2) thalline that obtains centrifugal collection thalline under 4 ℃, 4000rpm condition, be diluted to 1.0 ~ 2.0 * 10 with SPSS
11Individual/ml is for subsequent use;
The preparation of described Lactobacillus plantarum bacteria suspension is:
1) the dull and stereotyped cultivation activates: Lactobacillus plantarum is inoculated in the dull and stereotyped cultivation of MRS, cultivates 24 ~ 36h under 30 ~ 40 ℃ of conditions, form single bacterium colony;
2) with the Lactobacillus plantarum bacterial classification inoculation of step 1) activation culture in the MRS Liquid Culture, under 30 ~ 40 ℃ of conditions, cultivate 12 ~ 24h, make it be in the logarithm middle and later periods;
3) with step 2) thalline that obtains centrifugal collection thalline under 4 ℃, 4000rpm condition, be diluted to 1.0-2.0 * 10 with SPSS
11Individual/ml is for subsequent use;
The by volume ratio mixing of 4:2:3 of above-mentioned three kinds of bacteria suspensions is obtained plastc ring.
2. fermented feed for piglets according to claim 1, it is characterized in that: the raw material by the following portions by weight ratio is made:
The plastc ring of 41 ~ 48 parts of corn flour, 32 ~ 39 parts of dregs of beans, 13 ~ 19 parts in wheat bran, 0.8 ~ 1.2 times of above-mentioned raw materials parts by weight; Described plastc ring is that the by volume ratio mixing of 4:2:3 of bafillus natto bacteria suspension, saccharomycete bacteria suspension and Lactobacillus plantarum bacteria suspension makes.
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