CN111165680A - Composite probiotic feed additive for geese and preparation method and application thereof - Google Patents
Composite probiotic feed additive for geese and preparation method and application thereof Download PDFInfo
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- CN111165680A CN111165680A CN202010150965.4A CN202010150965A CN111165680A CN 111165680 A CN111165680 A CN 111165680A CN 202010150965 A CN202010150965 A CN 202010150965A CN 111165680 A CN111165680 A CN 111165680A
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- 239000006041 probiotic Substances 0.000 title claims abstract description 74
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 74
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 73
- 239000002131 composite material Substances 0.000 title claims abstract description 56
- 239000003674 animal food additive Substances 0.000 title claims abstract description 39
- 238000002360 preparation method Methods 0.000 title claims abstract description 34
- 241000272517 Anseriformes Species 0.000 title claims abstract 18
- 241000272814 Anser sp. Species 0.000 claims abstract description 26
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 16
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 claims abstract description 16
- 235000013379 molasses Nutrition 0.000 claims abstract description 16
- 235000013923 monosodium glutamate Nutrition 0.000 claims abstract description 16
- 229940073490 sodium glutamate Drugs 0.000 claims abstract description 16
- 239000004202 carbamide Substances 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 14
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 14
- 241000194032 Enterococcus faecalis Species 0.000 claims abstract description 14
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 14
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 14
- 229940032049 enterococcus faecalis Drugs 0.000 claims abstract description 14
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 14
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 11
- 239000008103 glucose Substances 0.000 claims abstract description 11
- 150000001875 compounds Chemical class 0.000 claims abstract description 10
- 235000013877 carbamide Nutrition 0.000 claims abstract description 7
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 6
- 238000006243 chemical reaction Methods 0.000 claims abstract description 6
- 239000002994 raw material Substances 0.000 claims abstract description 6
- 238000000855 fermentation Methods 0.000 claims description 21
- 230000004151 fermentation Effects 0.000 claims description 21
- 238000002156 mixing Methods 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 12
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 8
- 238000009395 breeding Methods 0.000 claims description 3
- 230000001488 breeding effect Effects 0.000 claims description 3
- 238000000034 method Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 239000002253 acid Substances 0.000 abstract description 2
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 230000007774 longterm Effects 0.000 abstract description 2
- 238000003860 storage Methods 0.000 abstract description 2
- 230000004083 survival effect Effects 0.000 abstract description 2
- 239000000654 additive Substances 0.000 abstract 1
- 230000000996 additive effect Effects 0.000 abstract 1
- 241000272808 Anser Species 0.000 description 48
- 230000002354 daily effect Effects 0.000 description 14
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000004615 ingredient Substances 0.000 description 4
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 229910052698 phosphorus Inorganic materials 0.000 description 3
- 239000011574 phosphorus Substances 0.000 description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 235000019750 Crude protein Nutrition 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 235000021050 feed intake Nutrition 0.000 description 2
- 230000003031 feeding effect Effects 0.000 description 2
- 244000144972 livestock Species 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 230000004584 weight gain Effects 0.000 description 2
- 235000019786 weight gain Nutrition 0.000 description 2
- 241000305071 Enterobacterales Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 229920001807 Urea-formaldehyde Polymers 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001363 autoimmune Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 210000003746 feather Anatomy 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000007413 intestinal health Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/33—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Birds (AREA)
- Mycology (AREA)
- Botany (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides a composite probiotic feed additive for geese and a preparation method and application thereof, belonging to the technical field of feed additives; the composite probiotic feed additive for the geese comprises the following raw materials for preparation: the composite probiotic preparation comprises molasses, sodium glutamate, urea and water; the compound probiotic preparation comprises the following components: bacillus subtilis, lactobacillus acidophilus, enterococcus faecalis, saccharomycetes and glucose. The invention relates to a composite probiotic feed for geeseThe effective viable count in the additive is 1 × 1010～1×1011cfu/g, strong environmental adaptability, long-term storage (more than 12 months) under anaerobic condition, survival and stability, and suitability for application in goose feed; in addition, the compound probiotic feed additive for geese has strong acid production capacity, can inhibit the growth of harmful substances, and can improve the daily gain of geese, reduce the feed conversion ratio and improve the feed utilization rate.
Description
Technical Field
The invention relates to the technical field of feed additives, in particular to a composite probiotic feed additive for geese and a preparation method and application thereof.
Background
The feed probiotic preparation is a novel feed additive, and can be used for improving the digestibility and the utilization rate of coarse feed and the production performance of livestock and poultry, reducing the excretion of nitrogen and phosphorus in livestock and poultry excrement and reducing the pollution to water and soil. In the prior art, CN104137956A discloses a goose feed comprising probiotic preparations, which has the effect of improving the autoimmune function of geese; CN105558273A discloses a special feed for laying geese, which comprises probiotic preparations and can increase the egg yield. At present, a special feed probiotic preparation for improving the daily gain of geese is lacked.
Disclosure of Invention
The invention aims to provide a composite probiotic feed additive for geese, and a preparation method and application thereof.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a composite probiotic feed additive for geese, which comprises the following raw materials in parts by mass for preparation: 8-12 parts of a composite probiotic preparation, 8-12 parts of molasses, 0.05-0.15 part of sodium glutamate, 0.05-0.15 part of urea and 75-85 parts of water;
the compound probiotic preparation comprises the following components: bacillus subtilis, lactobacillus acidophilus, enterococcus faecalis, saccharomycetes and glucose; the mass ratio of the bacillus subtilis to the lactobacillus acidophilus to the enterococcus faecalis to the yeasts to the glucose is 30-40: 35-45: 3-8: 8-12: 8-12;
the effective viable count of the goose composite probiotic feed additive is 1 multiplied by 1010～1×1011cfu/g。
Preferably, the pH value of the goose composite probiotic feed additive is 3.0-4.5.
The invention also provides a preparation method of the goose composite probiotic feed additive, which comprises the following steps:
1) mixing molasses, sodium glutamate, urea and water to obtain a mixture;
2) and mixing the composite probiotic preparation and the mixture, and carrying out sealed fermentation for 10-15 d at the temperature of 35-40 ℃ to obtain the composite probiotic feed additive for the goose.
Preferably, the sealed fermentation process in the step 2) is accompanied by stirring treatment; the stirring speed is 70-150 rpm, and the stirring time is 30-60 min per day.
The invention provides application of the composite probiotic feed for geese in the scheme in breeding of the seed geese.
Preferably, the application comprises the steps of increasing the daily gain of the seed geese, reducing the feed conversion ratio or improving the feed utilization rate.
Preferably, the application comprises the following steps: mixing the composite probiotic feed for the geese into basic ration of the geese, and feeding the geese; the mass ratio of the composite probiotic feed for the geese to the basic ration of the geese is 1-3: 1000.
preferably, the mass ratio of the composite probiotic feed for the geese to the basic ration of the geese is 2: 1000.
the invention has the beneficial effects that: the invention provides a composite probiotic feed additive for geese, which comprises the following raw materials for preparation: the composite probiotic preparation comprises molasses, sodium glutamate, urea and water; the compound probiotic preparation comprises the following components: bacillus subtilis, lactobacillus acidophilus, enterococcus faecalis, saccharomycetes and glucose. The effective viable count of the composite probiotic feed additive for geese is 1 multiplied by 1010～1×1011cfu/g, strong environmental adaptability, long-term storage (more than 12 months) under anaerobic condition, survival and stability, and suitability for application in goose feed; in addition, the compound probiotic feed additive for geese has strong acid production capability, can inhibit the growth of harmful substances,can improve the daily gain of the geese, reduce the feed conversion ratio and improve the feed utilization rate.
Detailed Description
The invention provides a composite probiotic feed additive for geese, which comprises the following raw materials in parts by mass for preparation: 8-12 parts of a composite probiotic preparation, 8-12 parts of molasses, 0.05-0.15 part of sodium glutamate, 0.05-0.15 part of urea and 75-85 parts of water; preferably; the composite probiotic feed additive for the geese comprises the following raw materials for preparation in parts by mass: 10 parts of composite probiotic preparation, 10 parts of molasses, 0.1 part of sodium glutamate, 0.1 part of urea and 79.8 parts of water.
In the invention, the molasses, the sodium glutamate and the urea are from conventional commercial sources; the molasses, the water, the sodium glutamate and the urea form a mixture which is used as a fermentation medium of the probiotics; in the invention, molasses provides energy (carbon source) for strain fermentation, sodium glutamate provides nitrogen source for strain fermentation, and urea provides nitrogen source for strain fermentation, thereby achieving the purpose of rapid fermentation.
In the invention, the compound probiotic preparation comprises the following components: bacillus subtilis, lactobacillus acidophilus, enterococcus faecalis, saccharomycetes and glucose; the mass ratio of the bacillus subtilis to the lactobacillus acidophilus to the enterococcus faecalis to the yeasts to the glucose is 30-40: 35-45: 3-8: 8-12: 8-12, preferably 35: 40: 5: 10: 10; the bacillus subtilis, the lactobacillus acidophilus, the enterococcus faecalis and the yeast are from conventional markets; the bacillus subtilis, lactobacillus acidophilus, enterococcus faecalis and yeast are preferably feed-grade microbial agents; the effective viable count of the bacillus subtilis, the lactobacillus acidophilus, the enterococcus faecalis and the yeast is preferably 1 × 1010～1×1011cfu/g; the glucose provides a carbon source, the four bacteria and the glucose are premixed in proportion and then fermented, and the effect is good.
In the invention, the three bacteria of bacillus subtilis, lactobacillus acidophilus and enterococcus faecalis are goose enterobacteria and beneficial bacteria, and can maintain intestinal health and promote growth; the yeast consumes oxygen and the strain ferments to provide nutrient substances.
In the present invention, theThe effective viable count of the goose composite probiotic feed additive is 1 multiplied by 1010～1×1011cfu/g。
In the invention, the pH value of the composite probiotic feed additive for geese is preferably 3.0-4.5, and more preferably 3.5-4.0; the strain can complete fermentation in the pH range, and the product can be stored for a long time.
The invention also provides a preparation method of the goose composite probiotic feed additive, which comprises the following steps:
1) mixing molasses, sodium glutamate, urea and water to obtain a mixture;
2) and mixing the composite probiotic preparation and the mixture, and carrying out sealed fermentation for 10-15 d at the temperature of 35-40 ℃ to obtain the composite probiotic feed additive for the goose.
Firstly, mixing molasses, sodium glutamate, urea and water to obtain a mixture; the invention has no special limitation on the mixing mode and time, and the uniform mixing is taken as the standard.
After the mixture is obtained, the composite probiotic preparation and the mixture are mixed, and sealed fermentation is carried out for 10-15 days at the temperature of 35-40 ℃ to obtain the composite probiotic feed additive for the goose; the temperature of the sealed fermentation is preferably 37 ℃; the time for the sealed fermentation is preferably 12 d; the sealed fermentation equipment is preferably a sealed fermentation tank; the preferable accompanying stirring treatment in the sealing fermentation process; the rotating speed of the stirring is preferably 70-150 rpm, and more preferably 80-100 rpm; the stirring time is preferably 30-60 min, and more preferably 40-50 min.
The invention provides the application of the composite probiotic feed for geese in the scheme in goose culture; the goose is preferably a gosling; the application preferably comprises the steps of increasing the daily gain of the geese, reducing the feed conversion ratio or improving the feed utilization rate. In the invention, the variety of the goose comprises Yangzhou goose; the age of the goose in days preferably comprises 1-60 days.
In the present invention, the application preferably comprises the steps of: mixing the composite probiotic feed for the geese into basic ration of the geese, and feeding the geese; the mass ratio of the composite probiotic feed for the geese to the basic ration of the geese is preferably 1-3: 1000, more preferably 2: 1000, parts by weight; the feeding amount is not particularly limited, and is subject to the conventional feeding amount in the field; the formula of the goose basic ration is not particularly limited, and the goose basic ration in the field can be used.
The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1 composite probiotic feed additive for geese
1. The components are as follows:
10 wt% of molasses, 10 wt% of composite probiotic agent (35 wt% of bacillus subtilis, 40 wt% of lactobacillus acidophilus, 5 wt% of enterococcus faecalis, 10 wt% of yeast and 10 wt% of glucose), 79.8 wt% of water, 0.1 wt% of sodium glutamate and 0.1 wt% of starter (urea).
2. The preparation method comprises the following steps:
1) strain: bacillus subtilis, lactobacillus acidophilus, enterococcus faecalis and saccharomycetes which are all feed-grade strains;
2) culture medium: molasses, water, sodium glutamate and urea;
3) adding molasses, sodium glutamate, urea and water into a closed fermentation tank, stirring uniformly, adding the composite probiotic bacteria agent into the closed fermentation tank, and continuously stirring at the stirring speed of 70r/min for 60min every day, wherein the fermentation temperature is 37 ℃ and the fermentation time is 12 d.
Example 2 feeding effect of the composite probiotic feed additive for geese of example 1 on the seed geese
160 Yangzhou goose goslings (not considering male and female goose) with 1-day old, clean and glossy feathers, uniform and healthy physique and quality are selected, the average initial weight is 88.27g, the goslings are randomly divided into 4 treatment groups after being weighed one by one in an empty stomach, each treatment group has 4 repetitions, and each repetition comprises 10 geese which are respectively a control group and a test group I, II and III. The control group is fed with basic daily ration (see table 1), and the group I, II and III are fed with 1, 2 and 3g/kg of goose composite probiotic feed additive respectively, and the test period is 30 d. Test site: yangtze township goose breeding farm in Guangdong province of Jiangsu.
TABLE 1 basic diet composition and nutritional ingredients
Note that crude protein, calcium and total phosphorus are measured values, and other indexes are calculated according to Chinese feed database-Chinese feed ingredient and nutritive value (revised 2000).
The test results are shown in table 2: the composite probiotic feed additive for geese at different levels in daily ration can obviously improve the daily gain of the seed geese, reduce the feed conversion ratio and improve the feed utilization rate, wherein the probiotic addition group with 2g/kg has the best effect. Daily gain of 1-7 days old, 8-15 days old and 16-30 days old, wherein the test groups are all obviously higher than the control group (P is less than 0.05); the group II of the 1-7-day-old daily gain test is remarkably higher than that of a control group (P is less than 0.01); daily gain of 8-15 days old and 16-30 days old, and the difference between probiotic test groups is not significant (P is more than 0.05). In each stage of the test period, the probiotic test group II is higher than the test groups I and III, and the daily gain is respectively increased by 6.89%, 4.50%, 5.35%, 3.63%, 4.71% and 0.60%. Compared with a control group, the feed intake of each test group at 1-7 days old and 8-15 days old is not significantly different (P is more than 0.05); the feed intake of 16-30 days old is obviously increased (P is less than 0.05) compared with that of a control group in each test group, and the difference between the test groups is not significant (P is more than 0.05). The material consumption weight gain ratio of each test group at 1-7 days old is obviously lower than that of a control group (P is less than 0.05), and the material consumption weight gain ratio of each test group at 8-15 days old and 16-30 days old has a reduced trend compared with that of the control group, but the difference is not obvious (P is more than 0.05).
TABLE 2 influence of composite probiotic feed additive for geese on growth performance of 30-day-old seed geese
Example 3 feeding effect of the composite probiotic feed additive for geese of example 1 on the seed geese
200 healthy 1-day-old young geese are selected, the average weight of the young geese after shelling is 89.36g, the young geese are randomly divided into 4 treatment groups, namely a control group and test groups I, II and III, each treatment group is 5 times in repetition, 10 geese are repeated in each treatment group, the control group is fed with basic ration (the formula is shown in a table 3), 1 g/kg, 2g/kg and 3g/kg of the concentrate of the test groups I, II and III are respectively fed with the composite probiotic feed additive for the geese prepared in the example 1, and the test period is 60 d. Test site: the animal husbandry institute of agriculture academy of sciences of Heilongjiang province innovates demonstration bases.
TABLE 3 basic diet composition and nutritional ingredients
Note that crude protein, calcium and total phosphorus are measured values, and other indexes are calculated according to Chinese feed database-Chinese feed ingredient and nutritive value (revised 2000).
The results are shown in Table 4 and show that: average daily gain of 1-15 days old, 16-30 days old, 31-45 days old and 46-60 days old, wherein the test groups are higher than the control group; at each stage of the test period, the compound probiotic test II group is higher than the test group I and the test group III, wherein the effect is obvious compared with that of a control group and the test group I at the age of 16-30 days, and the daily gain is respectively improved by 42.13% and 30.21%.
TABLE 4 influence of composite probiotic feed additive for geese on average daily gain of 60-day-old seed geese (g.d)-1)
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (8)
1. A composite probiotic feed additive for geese comprises the following raw materials for preparation in parts by mass: 8-12 parts of a composite probiotic preparation, 8-12 parts of molasses, 0.05-0.15 part of sodium glutamate, 0.05-0.15 part of urea and 75-85 parts of water;
the compound probiotic preparation comprises the following components: bacillus subtilis, lactobacillus acidophilus, enterococcus faecalis, saccharomycetes and glucose; the mass ratio of the bacillus subtilis to the lactobacillus acidophilus to the enterococcus faecalis to the yeasts to the glucose is 30-40: 35-45: 3-8: 8-12: 8-12;
the effective viable count of the goose composite probiotic feed additive is 1 multiplied by 1010～1×1011cfu/g。
2. The compound probiotic feed additive for geese according to claim 1, wherein the pH value of the compound probiotic feed additive for geese is 3.0-4.5.
3. The preparation method of the composite probiotic feed additive for geese of claim 1 or 2, comprising the following steps:
1) mixing molasses, sodium glutamate, urea and water to obtain a mixture;
2) and mixing the composite probiotic preparation and the mixture, and carrying out sealed fermentation for 10-15 d at the temperature of 35-40 ℃ to obtain the composite probiotic feed additive for the goose.
4. The method according to claim 3, wherein the sealed fermentation in step 2) is accompanied by stirring; the stirring speed is 70-150 rpm, and the stirring time is 30-60 min per day.
5. The use of the composite probiotic feed for geese according to claim 1 or 2 in goose breeding.
6. The use according to claim 5, wherein the use comprises increasing the daily gain of geese, reducing the feed conversion ratio or increasing the feed utilization rate.
7. The application according to claim 5, characterized in that it comprises the following steps: mixing the composite probiotic feed for the geese into basic ration of the geese, and feeding the geese; the mass ratio of the composite probiotic feed for the geese to the basic ration of the geese is 1-3: 1000.
8. the use of claim 7, wherein the mass ratio of the composite probiotic feed for geese to the basic ration for geese is 2: 1000.
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| CN202010150965.4A CN111165680A (en) | 2020-03-06 | 2020-03-06 | Composite probiotic feed additive for geese and preparation method and application thereof |
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| CN113116969A (en) * | 2021-05-25 | 2021-07-16 | 湾区生物技术(广州)有限公司 | Compound antibiotic-free feed additive and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101803682A (en) * | 2009-12-30 | 2010-08-18 | 沈阳科丰牧业科技有限公司 | Compound microbial agent and production method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101803682A (en) * | 2009-12-30 | 2010-08-18 | 沈阳科丰牧业科技有限公司 | Compound microbial agent and production method and application thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113116969A (en) * | 2021-05-25 | 2021-07-16 | 湾区生物技术(广州)有限公司 | Compound antibiotic-free feed additive and preparation method and application thereof |
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Application publication date: 20200519 |