CN111088194B - Enterococcus faecalis and application thereof - Google Patents
Enterococcus faecalis and application thereof Download PDFInfo
- Publication number
- CN111088194B CN111088194B CN202010044764.6A CN202010044764A CN111088194B CN 111088194 B CN111088194 B CN 111088194B CN 202010044764 A CN202010044764 A CN 202010044764A CN 111088194 B CN111088194 B CN 111088194B
- Authority
- CN
- China
- Prior art keywords
- enterococcus faecalis
- cctcc
- application
- enterococcus
- survival rate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/46—Streptococcus ; Enterococcus; Lactococcus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/40—Feeding-stuffs specially adapted for particular animals for carnivorous animals, e.g. cats or dogs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Animal Husbandry (AREA)
- Organic Chemistry (AREA)
- Food Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Birds (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Physiology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Enterococcus faecalis and application thereof. The Enterococcus faecalis (Enterococcus faecalis) is preserved in the China center for type culture Collection in 2019, 12 and 31 months, and the preservation number is CCTCC NO: m20191139. The application of the bacillus coagulans refers to that the enterococcus faecalis CCTCC NO: m20191139 is used for animal feeding, and the dosage of single fungus powder with the specification of 100 hundred million cfu/g is 0.5-1.0 per mill. The invention has the beneficial effects that the enterococcus faecalis CCTCC NO: the M20191139 has good stability and can obviously improve the health status of animals.
Description
Technical Field
The invention relates to enterococcus faecalis and application thereof in feed additives, and belongs to the technical field of microorganisms.
Background
Probiotics generally refer to microbial agents and metabolites thereof that improve the microbial balance of a host to exert beneficial effects and achieve the purpose of improving the health level and state of the host. Enterococcus faecalis: (Enterococcus faecalis ) One of the lactic acid bacteria, gram-positive bacteria, is one of the main groups of bacteria in the intestinal tracts of humans and animals.
The probiotic mechanism of action of enterococcus faecalis includes: (1) as one of lactic acid bacteria, enterococcus faecalis can produce bacteriocin, organic acid or volatile fatty acid and the like to improve the environment in the intestinal tract, so as to achieve the effects of competing for the application components of harmful bacteria or inhibiting the growth and activity of the harmful bacteria; (2) enterococcus faecalis can form a biological film in the intestinal tract of an animal and attach to the intestinal mucosa of the animal, so that the normal development and growth reproduction can be carried out, a lactic acid bacteria barrier can be formed, and the invasion of foreign toxins and pathogenic bacteria can be resisted; (3) enterococcus faecalis can convert nitrogen-free extracts of various carbohydrates into lactic acid which can be completely absorbed and utilized by organisms; can also decompose part of protein; (4) enterococcus faecalis can remarkably enhance the activity of macrophages, promote the development of immune organs, improve the immune response capability of animals, and promote the generation of antibodies and the secretion of cytokines, thereby enhancing the immunity of organisms (Anyuting, Huhao, Wangzhi, Zhouxiexiu, the biological characteristics of enterococcus faecalis and the application thereof in the breeding industry, Anhui agricultural science 2015, 43 (27): 115-117).
In feed additive variety catalog (2013), enterococcus faecalis is classified as a microbial feed additive, and is applicable to cultured animals. There are many reports on the application of enterococcus faecalis in livestock and poultry breeding. CN104293697B discloses a chicken feed probiotic containing enterococcus faecalis and a preparation method thereof, and the beneficial effects are that: the feed utilization efficiency of animals is improved, the cost is saved, the steady state of the internal digestive tract environment of the animals is greatly improved, the absorption and utilization of nutrition are promoted, the growth of the animals is promoted, the production performance, the immunity performance and the reproductive performance of the animals are obviously improved, the production cost is reduced, and the economic benefit is improved. CN110326706A discloses a compound high-activity probiotics for feeding containing enterococcus faecalis, which has the beneficial effects that: the probiotic agent can effectively improve the utilization rate of feed and the immunity of the cultured animal body, reduce the probability of diarrhea and diarrhea, improve the color and luster of animal fur and the quality of meat, reduce the odor of animal excrement, reduce the medical administration times and reduce the animal feeding cost.
Most lactic acid bacteria can not resist the action of adverse environmental conditions such as gastric acid, bile salt and the like in the digestive tract of animals, and can not resist high temperature, pressure and shearing force during granulation in the processing of granulated feeds, and can be easily influenced by temperature, extrusion and oxygen in the transportation and storage processes, so that the activity is greatly reduced and even dies, and the titer of the live bacteria preparation and the due probiotic characteristics thereof are seriously influenced (Wangjianhua, Yang New construction, Tengda, WangXiuMin, Mao-Ruiyu, Haia, a enterococcus faecalis microcapsule and a preparation method thereof, CN 107260704A). Enterococcus faecalis also suffers from the same problem. The room temperature stability of 3 domestic similar products is inspected by Jiang Wen Tao and the like, and the survival rate is found to be not more than 32% at most in 60 days (the influence factors of the stability of Jiang Wen Tao, Maxam, Jiapunhui, Rugang, Qiubu and micro-encapsulation enterococcus faecalis are research, and the research on feed shows that the 01 th stage in 2018 is 74-78).
Numerous attempts have been made by researchers to enhance the stability of enterococcus faecalis. Zhang Lin et al prepared microencapsulated enterococcus faecalis by emulsification gelation, found that the survival rate of microencapsulated enterococcus faecalis 5 months was increased from 10.96% to 30.42%, and the resistance to adverse environment was significantly improved (Zhang YuTing, extremely waves, Lijie, WangWei, Lieike, research on the anti-stress action of microencapsulated probiotic bacteria by emulsification gelation, report on animal nutrition, 11 th 2015: 3636 one 3642). In vitro characteristics of microencapsulated enterococcus faecalis are researched by Duchen and the like, and the in vitro characteristics of the microencapsulated enterococcus faecalis are found to be obviously improved compared with a bacterium powder sample, and the survival rate of the microencapsulated enterococcus faecalis is increased from 31.5% to 65.9% in 6 months (research on in vitro characteristics of Duchen Lin, Yi Wang, Lixue Ping and microencapsulated enterococcus faecalis, Chinese feed additive, 7 th stage in 2018: 36-39). The survival rate of the micro-encapsulated enterococcus faecalis reaches 96.6 percent in 45 days and is obviously improved compared with 11.3 percent of the original bacterial powder by selecting a proper carrier and controlling the moisture; in pilot plant experiments, the 30-day survival rate and the 60-day survival rate of the microencapsulated enterococcus faecalis can respectively reach 88.7 percent and 81.3 percent (Jiang Wen, Majiaarmy, Jiapunhui, Rugang, Qiongong, research on stability influencing factors of the microencapsulated enterococcus faecalis, feed research, 2018, stage 01: 74-78).
Based on cost factors and processing convenience, the feeding probiotics not only require significant probiotic functions, but also must have good processing performance and storage performance.
Disclosure of Invention
In order to solve the problems in the prior art, the applicant of the invention provides a enterococcus faecalis strain and application thereof in feed processing. The enterococcus faecalis strain has strong stress resistance and good stability, and can be used as animal probiotics.
The technical scheme of the invention is as follows:
enterococcus faecalis: (Enterococcus faecalis )014 and application thereof.
Said enterococcus faecalis: (Enterococcus faecalis )014, it is preserved in China center for type culture Collection in 2019, 12 months and 31 days, with the preservation number of CCTCC M20191139.
The application of enterococcus faecalis refers to the application of enterococcus faecalis CCTCC NO: m20191139 is used for feeding animals, and the dosage of single fungus powder with the specification of 100 hundred million cfu/g is 0.5-1.0 per mill.
The invention has the beneficial effects that:
the enterococcus faecalis CCTCC NO of the invention: the M20191139 has good stability, and can obviously improve the health status of animals.
Detailed Description
The present invention will be described in detail with reference to examples.
The invention relates to enterococcus faecalisEnterococcus faecalis )014 it is preserved in China center for type culture Collection in Wuhan, China in 2019, 12 months and 31 days, with the preservation number of CCTCC NO: m20191139.
Example 1
Taking enterococcus faecalis CCTCC NO: the M20191139 fermentation broth was evaluated for acid resistance.
The viable count of the enterococcus faecalis fermentation liquor is 50 hundred million cfu/mL. 1mL of enterococcus faecalis fermentation liquid is added into simulated gastric juice, and cultured at 37 ℃. And sampling at regular time, measuring the viable count of the enterococcus faecalis, comparing the viable count with the initial viable count, and calculating the survival rate.
The preparation method of the simulated gastric fluid comprises the following steps: 100mL of diluted hydrochloric acid solution with the pH value of 1.5-3.0 are prepared respectively, and 1g of pepsin is added respectively to ensure that the pepsin is fully dissolved and simulates gastric juice.
And (3) determining the viable count of the enterococcus faecalis by adopting a pour plate method. Ten times of dilution is carried out by sterile normal saline, 1mL of diluent is taken on a culture dish, MRS culture medium which is cooled to 45 ℃ after sterilization is poured on the culture dish, the culture dish is quickly shaken up and is cultured for 24 hours at 37 ℃, and the quantity of enterococcus faecalis is calculated. The MRS culture medium preparation method comprises the following steps: 10.0g of peptone, 5.0g of beef powder, 4.0g of yeast powder, 20.0g of glucose, 801.0mL of Tween, 2.0g of dipotassium phosphate heptahydrate, 5.0g of sodium acetate trihydrate, 2.0g of triammonium citrate, 0.2g of magnesium sulfate heptahydrate, 0.05g of manganese sulfate tetrahydrate and 15.0g of agar powder, adding the components into 1000mL of distilled water, heating for dissolving, adjusting the pH value to be 6.2 +/-0.2, and sterilizing at 121 ℃ for 15-20 min.
Table 1 enterococcus faecalis CCTCC NO: survival rate of M20191139 in different simulated gastric acid conditions
Gastric juice of human and animals is strongly acidic, and simulated gastric juice is adopted to evaluate the content of enterococcus faecalis CCTCC NO: acid resistance of M20191139. pH1.5-3.0, and the CCTCC NO: the survival rate of M20191139 decreased gradually; the lower the pH, the more pronounced the tendency to decline. Even then, after 180min of action at pH1.5, enterococcus faecalis CCTCCNO: the survival rate of M20191139 still reaches 58%, which shows that the CCTCC NO: m20191139 has good acid resistance.
Example 2
Taking enterococcus faecalis CCTCC NO: the M20191139 fermentation liquor was evaluated for its bile salt resistance.
The viable count of the enterococcus faecalis fermentation liquor is 50 hundred million cfu/mL. Adding 1mL of enterococcus faecalis fermentation liquor into simulated bile salt, and culturing at 37 ℃. And sampling at regular time, measuring the viable count of the enterococcus faecalis, comparing the viable count with the initial viable count, and calculating the survival rate.
The preparation method of the simulated bile salt comprises the following steps: 8g of sodium chloride, 0.2g of potassium chloride, 3.63g of disodium hydrogen phosphate dodecahydrate, 0.24g of monopotassium phosphate, 1000mL of purified water and 0.1-0.3% of pig bile salt.
The method for measuring the viable count of enterococcus faecalis and the method for calculating the viable count of enterococcus faecalis are the same as in example 1.
Table 2 enterococcus faecalis CCTCC NO: survival rate of M20191139 in different simulated bile salt conditions
Bile salts are destructive to microorganisms. To be probiotic, the microorganisms must be tolerant to bile salts. The concentration of bile salt in human body is generally 0.3%, and animals are different. Under the condition of simulating a bile salt test, the content of enterococcus faecalis CCTCC NO: the survival rate of M20191139 gradually decreased; the higher the bile salt concentration, the more pronounced the downward trend. 0.5% bile salt, after 6hr, enterococcus faecalis CCTCC NO: the survival rate of M20191139 still reaches 79%, which shows that the CCTCC NO: m20191139 has good bile salt resistance.
Example 3
For enterococcus faecalis CCTCC NO: the storage stability of the M20191139 single-strain powder was evaluated.
Mixing enterococcus faecalis original bacteria powder and anhydrous glucose in proportion to prepare single enterococcus faecalis bacteria powder (specification: 500 hundred million cfu/g-2000 hundred million cfu/g); sealing the single bacterium powder, storing at-18 deg.C, 4 deg.C and room temperature respectively, periodically measuring viable count, and calculating survival rate.
Table 3 enterococcus faecalis CCTCC NO: survival rate of M20191139 single-strain powder at different storage temperatures
The survival rates of enterococcus faecalis single powder with different specifications at different storage temperatures are slightly different, but the conclusion is consistent: it is easy to store at low temperature. From the test situation, the enterococcus faecalis CCTCC NO: the single M20191139 bacterial powder is stored for 2 months at room temperature, and the survival rate is kept above 80%; the survival rate is kept at about 70 percent after the storage for 3 months; the survival rate is not less than 60 percent after 6 months of storage. Thus, enterococcus faecalis CCTCC NO: the finished product of the M20191139 single-fungus powder has relatively long shelf life and is convenient for customers to use.
Example 4
And (2) adding enterococcus faecalis CCTCC NO: m20191139 single-strain powder (specification: 100 hundred million cfu/g) is used for feeding piglets.
108 weaned piglets with average weight of 28 days old (7.82 +/-0.75) kg are selected and randomly divided into 3 groups, each group has 6 repetitions (each of the male and female accounts for half), each repetition has 6 repetitions, and each repetition has 1 column. Group I is blank group, and no probiotics and antibiotics are added into basic diet; group II is probiotic group, and the content of enterococcus faecalis CCTCC NO: m20191139 single-strain powder is mixed into basic feed, and the dosage is 1 per mill of the feeding amount; group III was the antibiotic group, with 500mg/kg colistin sulfate added to the basal diet. The piglets are raised in a closed piggery, are fed on cement ground, are fed and drunk freely, and are subjected to parasite expelling, disinfection and immunization according to the conventional raising management program of a pig farm, and the test period is 30 days. The calculation method of the diarrhea rate of the piglets is carried out according to the literature (Stroke, Yaohuansu, Jiangshan, Xiaoshui, Liuwuhua, Populicayun, Dongzhong, Guojun, the influence of enterococcus faecalis on the growth performance, the diarrhea rate, the blood biochemical index and the immune organ of the weaned piglets by replacing antibiotics, reports on animal nutrition 2015, 27 (6): 1832-:
table 4 enterococcus faecalis CCTCC NO: effect of M20191139 on piglet diarrhea Rate
| Group I | Group II | Group III | |
| Rate of diarrhea | 7.3％ | 1.5％ | 5.2％ |
Group I, group II, group III diarrhea rate 7.3%, 1.5%, 5.2%
Enterococcus faecalis CCTCC NO: m20191139 can remarkably reduce the diarrhea rate of piglets and has positive effect on promoting the healthy growth of the piglets.
Example 5
And (2) carrying out the culture of enterococcus faecalis CCTCC NO: m20191139 Single-strain powder (specification: 100 hundred million cfu/g) is used for feeding pet dogs.
Referring to Zhangingxiu method (Zhangingxiu, preparation and application research of high polyunsaturated fatty acid content dog food [ D ], Wuxi: university in south of the Yangtze river, 2013), 45 adult beagle dogs meeting the health requirements of experimental animals, 10kg of the adult beagle dogs are selected and randomly divided into 3 groups, and each group comprises 15 dogs. The breeding temperature is 23 +/-2 ℃, the relative humidity is 55 +/-5%, the illumination time is 7: 00-19: 00, and the breeding is carried out in one cage. Commercial dog food (full-value dog food of small and medium-sized baoluo adult dogs) is adopted to feed beagles according to the recommended feeding amount, and after three weeks, the beagles are randomly divided into 3 groups (a control group, an experimental group I and an experimental group II) to be fed respectively. The feeding time was 9 weeks. The control group was fed normal commercial dog food; in the first experimental group, the feed is mixed and fed according to the using amount of 1 per thousand; and in the second experimental group, the feed is mixed and fed according to the use amount of 0.5 per mill.
Collecting dog hair: days-21, -14, 0, 7, 14, 28, and 63. Scoring by sensory evaluation according to the Zhangming method: 1-dull, rough, dry; 2-not curved, not soft; 3-moderately full, moderately soft; 4-highly curved, very flexible; 5-greasy.
Table 5 enterococcus faecalis CCTCC NO: effect of M20191139 on dog Hair
In the state that NO enterococcus faecalis CCTCC NO: before M20191139, the scores of the control group, the first experimental group and the second experimental group are very close and are not basically distinguished, which indicates that the hair of the dogs is not different. Enterococcus faecalis CCTCC NO: after only 7 days of M20191139, the scores of the experimental group I and the experimental group II are improved compared with those of the control group; enterococcus faecalis CCTCC NO: after M2019113914 days, the scores of the experimental group I and the experimental group II are obviously higher than those of the control group. This indicates enterococcus faecalis CCTCC NO: m20191139 has obvious promotion effect on improving dog hair.
Claims (2)
1. Enterococcus faecalis strainEnterococcus f aecalis)014, characteristic ofThe method comprises the following steps: the enterococcus faecalis is enterococcus faecalis (A)Enterococcus faecalis )014, preservation number is CCTCC NO: m20191139.
2. The use of enterococcus faecalis according to claim 1 in animal feeding, wherein the amount of the single bacterial powder with the specification of 100 hundred million cfu/g is 0.5-1.0 per thousand.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010044764.6A CN111088194B (en) | 2020-01-16 | 2020-01-16 | Enterococcus faecalis and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010044764.6A CN111088194B (en) | 2020-01-16 | 2020-01-16 | Enterococcus faecalis and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111088194A CN111088194A (en) | 2020-05-01 |
| CN111088194B true CN111088194B (en) | 2022-09-20 |
Family
ID=70399129
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010044764.6A Active CN111088194B (en) | 2020-01-16 | 2020-01-16 | Enterococcus faecalis and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111088194B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113278560A (en) * | 2021-06-15 | 2021-08-20 | 无锡拜弗德生物科技有限公司 | Microecological preparation for improving pet odor |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI417054B (en) * | 2010-06-15 | 2013-12-01 | Jen Shine Biotechnology Co Ltd | Novel enterococcus faecium ljs-01 and its use for probiotic |
| CN102277325B (en) * | 2011-08-12 | 2013-03-13 | 北京金泰得生物科技股份有限公司 | Enterococcus faecalis strain for feed purpose and use thereof |
| CN104293696B (en) * | 2014-09-16 | 2016-08-17 | 李雪平 | One strain enterococcus faecalis HEW-A131 and application thereof |
| CN104522648B (en) * | 2014-12-18 | 2016-10-05 | 杭州远大生物制药有限公司 | A kind of tetrad probiotics preparation and application |
| CN104774782B (en) * | 2014-12-25 | 2017-12-19 | 东北农业大学 | Chicken source enterococcus faecalis separation strains and its application |
| CN107260704B (en) * | 2017-06-08 | 2020-02-11 | 中国农业科学院饲料研究所 | Enterococcus faecalis microcapsule and preparation method thereof |
| CN108251335B (en) * | 2018-01-18 | 2020-12-01 | 海南大学 | Enterococcus faecalis HKF7 with lactic acid activity and screening culture method and application thereof |
-
2020
- 2020-01-16 CN CN202010044764.6A patent/CN111088194B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN111088194A (en) | 2020-05-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102559550B (en) | Clostridium butyricum having diarrhea treating effect and application of clostridium butyricum as substitute of sodium butyrate | |
| CN107815429B (en) | Granulation-resistant lactobacillus preparation and preparation method thereof | |
| AU2013322644B2 (en) | Probiotic and prebiotic compositions | |
| CN110604226A (en) | Diarrhea-preventing growth-promoting composite feed additive for weaned piglets and preparation method thereof | |
| CN106260504B (en) | Method for producing microbial fermentation wet feed by using beer yeast paste | |
| CN103535323A (en) | Livestock and poultry breeding mode | |
| CN101638627A (en) | Bacillus subtilis and application thereof in biological feed additives | |
| JP2017533197A (en) | Probiotic composition and prebiotic composition | |
| CN111802514A (en) | Microbial modifier and preparation method and application thereof | |
| JP2021506327A (en) | Animal feed material | |
| CN113717881A (en) | Bacillus coagulans BC66 and application thereof | |
| JP5499231B2 (en) | Animal feed composition containing Lactobacillus plantarum, combined animal feed containing the composition, and method for maintaining or growing the Lactobacillus plantarum in the animal intestine | |
| CN111088194B (en) | Enterococcus faecalis and application thereof | |
| KR100857771B1 (en) | Compositions for addition to feed for fish comprising Bacillus polyfermenticus, Bacillus licheniformis and Saccharomyces serevisiae | |
| CN106578354A (en) | Piglet compound premix, preparation method and daily ration for piglet | |
| CN107043724B (en) | Bacillus licheniformis and separation method and application thereof | |
| CN102334602A (en) | Preparation method of bioactive composite high-efficiency feed nutrient solution used for livestock and poultry | |
| CN111000033A (en) | Bacillus subtilis viable bacteria fermented traditional Chinese medicine preparation containing thyme oil and traditional Chinese medicine, preparation process and application | |
| Litonina et al. | Application of enzyme probiotic drug developed based on microorganisms of the rumen of reindeer (Rangifer tarandus) in feeding cows | |
| CN102823771A (en) | Biological active acidifying agent for piglets, premix compound and compound | |
| CN114032200A (en) | Preparation method of bacillus subtilis feed additive for breeding animals | |
| CN114424800A (en) | Feed additive for improving intestinal health index of broiler chickens and application thereof | |
| RU2378000C2 (en) | Method for production of therapeutic additive | |
| RU2326692C2 (en) | Probiotic medicinal agent for agricultural animals and birds | |
| CN109486713B (en) | Liquid composite lactobacillus preparation and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: Room 306, building 1, No. 2018 Lihu Avenue, Binhu District, Wuxi City, Jiangsu Province Applicant after: Wuxi baifude Biotechnology Co.,Ltd. Address before: 214024 floor 4, building 3, Xinlian Park, No. 999, Nanhu Avenue, Liangxi District, Wuxi City, Jiangsu Province Applicant before: Wuxi baifude Biotechnology Co.,Ltd. |
|
| CB02 | Change of applicant information | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20210728 Address after: 211101 room 1809, building A-1, Greenland window Business Plaza, Dongshan street, Jiangning District, Nanjing, Jiangsu Province Applicant after: Shengjinhe Biotechnology (Jiangsu) Co.,Ltd. Address before: Room 306, building 1, 2018 Lihu Avenue, Binhu District, Wuxi City, Jiangsu Province, 214122 Applicant before: Wuxi baifude Biotechnology Co.,Ltd. |
|
| TA01 | Transfer of patent application right | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |