CN102379374A - Microbial high-activity preparation, high-activity microbial protein forage and preparation method thereof - Google Patents
Microbial high-activity preparation, high-activity microbial protein forage and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to a microbial high-activity preparation, a high-activity microbial protein forage and a preparation method thereof. The microbial high-activity preparation comprises the following components: 10%-20% of photosynthetic bacteria, 20%-40% of lactobacilli, 10%-20% of Saccharomyces cerevisiae Hansen, 20%-30% of bacillus subtilis, and 10%-20% of bifidobacteria. The microbial protein forage comprises the following components: dried fish, blood powder, soybean, soybean meal, peanut meal, DDGS forage, calcium hydrogen phosphate, salt, soybean oligosaccharides, microbial high-activity preparation and vitamin composite premix. In preparation, high-temperature puffing and stirring are performed on the dried fish, blood powder and soybean. The components such as soybean meal, peanut meal and DDGS forage are added. The microbial high-activity preparation is sprayed for being added, and then vacuum packaging is performed. The microbial preparation can also be adopted for preparing mixed forage for grices. The microbial high-activity preparation of the invention can be fed directly and has high feeding safety. The microbial high-activity preparation can be widely used for ecological cultivation of livestock, poultry and aquatic product. The high-activity microbial protein forage has the following advantages: improving forage conversion rate of the pig, preventing disease and treating disease, improving survival rate, purifying environment, reducing breeding cost, obtaining obvious yield and income increasing effect, and obtaining good popularization value.
Description
Technical field
The present invention relates to a kind of microorganism high activity preparation and feed, relate in particular to a kind of microorganism high activity preparation and high-activity microorganism protein feed and preparation method thereof.
Background technology
The use of some growth promotion health-care agents has promoted Developing of Animal Industry in the aquaculture, has brought many potential threats to human health simultaneously.Additives such as abuse of antibiotics in feed and the livestock and poultry cultivation, chemical synthetic drug, hormone, beta-stimulants, heavy metal and sedative have produced a series of problems and consequence at present, as: residual the exceeding standard of (1) animal products Chinese traditional medicine directly is detrimental to health.(2) the animal products quality descends, lacking of market competition ability and trust.Arsenic preparation such as widely used atoxylic acid in feed at present, research institution classifies carcinogen as by international cancer.In addition, at the medium-term and long-term sedative etc. that uses of pig industry, cause medicament residue.(3) bacterial drug resistance enhancing, antibody-resistant bacterium increase, and threaten human health.Sanitationist is thought, through the various resistance to the action of a drug germs that livestock produces, can pass to the people through food, and produces the disease that is difficult to healing.Simultaneously; The antibiotic that aureomycin, terramycin, chloramphenicol, olaquindox, sulfonamide, horse Du mycin etc. are easy residual and toxicity is bigger and a large amount of uses of synthesising bacteria anti-reflecting medicine; Cause medicament residue to increase, antibody-resistant bacterium diffusion, animal flora imbalance; The immunity of animal is suppressed, the secondary superinfection.(4) cause environmental destruction.Environmental protection becomes the problem that people are concerned about just day by day.The pollution that the intensification Production of Livestock and Poultry causes has become the key factor of restriction Production of Livestock and Poultry.In the intensive culture, livestock and poultry are discharged a large amount of ammoniacal nitrogens and phosphorus etc., influence the growth of plant through the Acidity of Aikalinity that changes water and soil earth.In addition, use high copper agent or arsenic preparation in the feed, also can be and polluted source and arable land through animal excrements.
In view of the problems that exist in above-mentioned feed and the livestock and poultry cultivation, in recent years, the consumer paid attention to food security, health and health especially, and was very strong to the requirement of food quality.Each country is just at active development green feed additive or green growth promotion health-care agent.Additive for microbe feedstuff; Natural, nontoxic with it, have no side effect, have no drug resistance, non-environmental-pollution; And can promote growth of animal and breeding, disease resistance, enhancing body immunity function, improve food conversion ratio and advantage such as animal feeding benefit, receive countries in the world raiser's well recognized.
At present, many Feed Enterprise are all at the additive for microbe feedstuff of actively seeking and study new environmental protection.But mostly the additive for microbe feedstuff on the market generally is single bacteria preparation, function singleness, and viable bacteria content is low in the product; Production technology and equipment are more backward; The living bacterial cells time-to-live is short, inferior quality, and living contaminants is serious; Result of use is undesirable, unstable, and quality can not satisfy consumer's needs.
Summary of the invention
The technical problem that the present invention will solve: be to overcome the defective that prior art exists, provide a kind of compatibility rationally, safety, non-harmful microorganism high activity preparation;
A kind of high-activity microorganism protein feed that can promote growth, reduce cost and preparation method thereof also is provided;
Also provide a kind of piglet with mixed feed and preparation method thereof.
Technical scheme of the present invention:
A kind of microorganism high activity preparation by weight percentage, comprises following component, photosynthetic bacteria 10%-20%, Bacillus acidi lactici 20%-40%, S. cervisiae 10%-20%, bacillus subtilis 20%-30%, Bifidobacterium 10%-20%.
Described microorganism high activity preparation, preferred: photosynthetic bacteria 10%-15%, Bacillus acidi lactici 30%-40%, S. cervisiae 15%-20%, bacillus subtilis 20%-25%, Bifidobacterium 10%-20%.
A kind of high-activity microorganism protein feed by weight percentage, comprises following component: dried fish 5%-10%, blood meal 8%-15%; Soybean 8%-15%, dregs of beans 35%-45%, peanut meal 8%-15%; DDGS feed 5-10%, calcium monohydrogen phosphate 5%-10%, salt 1-1.5%; Soyabean oligosaccharides 3%-10%, microorganism high activity preparation 1%-3%, vitamin compound premix 1%-3%.
Said vitamin compound premix is: in every kilogram of vitamin compound premix, vitamin A is not less than that 336000IU, vitamin D are not less than 5200 IU, vitamin E is not less than 480IU, vitamin K
3Be not less than 40mg, Cobastab
1Be not less than 54.4mg, Cobastab
2Be not less than 160mg, Cobastab
6Be not less than 120mg, Cobastab
12Being not less than 0.64mg, calcium pantothenate is not less than 320mg, nicotinic acid and is not less than 140mg, folic acid and is not less than 16mg, biotin and is not less than 1.28mg, choline and is not less than 12.5mg, iron and is not less than 1750mg, zinc and is not less than 1750mg, copper and is not less than 112.5mg, manganese and is not less than 75mg, iodine and is not less than 3.5mg, selenium and is not less than 7mg, cobalt and is not less than 5mg, lysine and is not less than that 50g, calcium are not less than 177g, total phosphorus is not less than 36g.
Also contain Chinese medicine 0.5%-1% in the said feed ingredient, traditional Chinese medicine ingredients is in weight portion, and wherein the Chinese bulbul is 0.625 part, 0.25 part in Radix Glycyrrhizae; 0.25 part of rheum officinale, 0.65 part of Radix Isatidis, 0.65 part of mother chrysanthemum, 0.4 part of cape jasmine; 0.35 part of betel nut, 0.4 part of Chinese violet, 0.35 part of alum; 0.2 part of realgar, 1.0 parts of rhizome of cyrtomium, 0.008 part of the dried venom of toads.
A kind of preparation method of high-activity microorganism protein feed mixes dried fish, blood meal, soybean, through high temperature puffing, pulverizes; Pour agitator tank then into, dregs of beans, peanut meal, DDGS feed are pulverized, pour in the above-mentioned agitator tank; Under agitation add calcium monohydrogen phosphate, salt, vitamin compound premix, stirred 10-15 minute, add soyabean oligosaccharides; Simultaneously the spraying of microorganism high activity preparation is added, stirred 10-15 minute, carry out vacuum packaging.
Adopt high-activity microorganism protein feed of the present invention can process complete feed, the formulation by weight of complete diet pellet is:
Corn 65-75%, wheat bran 6-20%, all the other are the high-activity microorganism protein feed, can raise the stage according to the difference of pig, carry out the adjustment of proportioning.
A kind of piglet is used mixed feed, by weight percentage, comprises following component: corn 30%-40%; Wheat bran 5%-10%, dregs of beans 10%-20%, soybean 10%-20%; Whey powder 10%-20%, fish meal 5%-10%, calcium monohydrogen phosphate 0.3%-1%; Soyabean oligosaccharides 3%-10%, microorganism high activity preparation 1%-3%, vitamin compound premix 1%-3%.
A kind of piglet is pulverized corn, wheat bran, dregs of beans, soybean, the expanded back of fish meal with the preparation method of mixed feed, in agitator tank, mixes; Under agitation add calcium monohydrogen phosphate, whey powder, vitamin compound premix; Stirred 10-15 minute, and added soyabean oligosaccharides, simultaneously the spraying of microorganism high activity preparation is added; Stirred 10-15 minute, and carried out vacuum packaging.
Various method for culturing microbes among the present invention:
1, the cultivation of photosynthetic bacteria
Its cultural method comprises the steps:
(1) dull and stereotyped cultivation rejuvenation: photosynthetic bacteria is adopted method streak inoculation on plating medium of streak inoculation; In 25～34 ℃, intensity of illumination 3000-4000lx, anaerobism was cultivated 12～50 hours, made the photosynthetic bacteria rejuvenation; Select typical bacterium colony, carry out morphology and CHARACTERISTICS IDENTIFICATION;
(2) culture medium preparation:
In w/v, ammonium chloride 2%, potassium dihydrogen phosphate 0.2%, sodium acetate 4%, sodium acid carbonate 2%, sodium chloride 1%, yeast extract 0.15%, magnesium sulfate 0.2%, distilled water 1000ml after above-mentioned substance mixes, transfers pH=6.5-7, and 120 ℃ of sterilizations of high pressure 20 minutes are subsequent use;
(3) preparation of seed liquor:
Colonies typical in the step (1) is inoculated in the above-mentioned culture medium, and in 25～34 ℃, intensity of illumination 3000-4000lx, anaerobism was cultivated 12～50 hours, obtained the seed liquor of photosynthetic bacteria;
(4) inoculated and cultured:
The volume ratio of above-mentioned seed liquor by 1-3% is inoculated in the culture medium, and in 25～39 ℃, intensity of illumination 2000-5000lx, anaerobism was cultivated 16～24 hours, measured the photosynthetic bacteria total bacteria count and was not less than 3,000,000,000/ml, and viable count is not less than 2,000,000,000/ml;
(5) collect bacterial classification:
Behind the no living contaminants of pure bacterium test, the centrifugal supernatant of abandoning is collected thalline then with above-mentioned culture.
2, the cultivation of Bacillus acidi lactici
Its cultural method comprises the steps:
(1) dull and stereotypedly cultivates rejuvenation: Bacillus acidi lactici is adopted method streak inoculation on plating medium of streak inoculation, cultivated 24～48 hours in 37～40 ℃, make the Bacillus acidi lactici rejuvenation, form single bacterium colony, carry out morphology and CHARACTERISTICS IDENTIFICATION;
(2) culture medium preparation:
In w/v, peptone 1%, beef extract 1.5%, glucose 2%; Manganese sulfate 0.25%, yeast soak powder 0.7%, magnesium sulfate 0.58%, agar 18%; Triammonium citrate 2.3%, potassium dihydrogen phosphate 0.4%, sodium acetate 0.3%, distilled water 1000ml; After above-mentioned substance mixes, transfer pH=5.5-5.8,120 ℃ of sterilizations of high pressure 15 minutes, subsequent use;
(3) preparation of seed liquor:
Qualified colony inoculation in the step (1) in above-mentioned culture medium, in 37～40 ℃ of cultivations 24～48 hours, is obtained the seed liquor of Bacillus acidi lactici;
(4) inoculated and cultured:
The volume ratio of above-mentioned seed liquor by 3-6% is inoculated in the culture medium,, measures Bacillus acidi lactici bacterium number and be not less than 1,000,000,000/ml in 37～40 ℃ of cultivations 16～60 hours;
(5) collect bacterial classification:
Behind the no living contaminants of pure bacterium test, the centrifugal supernatant of abandoning is collected thalline then with above-mentioned culture.
3, the cultivation of S. cervisiae
Its cultural method comprises the steps:
(1) dull and stereotypedly cultivates rejuvenation: S. cervisiae is adopted method streak inoculation on plating medium of streak inoculation, cultivated 24～30 hours in 26～30 ℃, make the S. cervisiae rejuvenation, form single bacterium colony, carry out morphology and CHARACTERISTICS IDENTIFICATION;
(2) culture medium preparation:
In w/v, sucrose 15%, ammonium carbonate 1%, potassium hydrogen phosphate 0.2%, calcium phosphate 0.02%, magnesium sulfate 0.02%, distilled water 1000ml, after above-mentioned substance mixes, 121 ℃ of sterilizations of high pressure 20 minutes, subsequent use;
(3) preparation of seed liquor:
The S. cervisiae of train qualified in the step (1) is inoculated in the above-mentioned culture medium,, obtains the seed liquor of S. cervisiae in 30～35 ℃ of cultivations 48～72 hours;
(4) inoculated and cultured:
The volume ratio of above-mentioned seed liquor by 1-5% is inoculated in the culture medium,, measures S. cervisiae bacterium number and be not less than 1,000,000,000/ml in 28～32 ℃ of cultivations 48～72 hours;
(5) collect bacterial classification:
Behind the no living contaminants of pure bacterium test, the centrifugal supernatant of abandoning is collected thalline then with above-mentioned culture.
4, the cultivation of bacillus subtilis
Its cultural method comprises the steps:
(1) dull and stereotypedly cultivates rejuvenation: bacillus subtilis is adopted method streak inoculation on plating medium of streak inoculation, cultivated 20～24 hours in 28～31 ℃, choose macrocolony; Be inoculated in the test tube that SPSS is housed, concussion evenly is positioned in 120 ℃ of baking ovens and heats 20min; Bacterium liquid after handling coated on the culture medium plate cultivated 20 hours; Gemma is germinateed, choose the macrocolony on the plate at last, carry out morphology and CHARACTERISTICS IDENTIFICATION;
(2) culture medium preparation:
In w/v, beef extract 3%, peptone 10%, sodium chloride 5%, agar 20%, glucose 15%, distilled water 1000ml, above-mentioned substance mixes, and heating for dissolving is transferred pH to 7.0-7.2, and 121 ℃ of sterilizations of high pressure 20 minutes are subsequent use;
(3) preparation of seed liquor:
Bacillus subtilis in the step (1) is inoculated in the above-mentioned culture medium, cultivated 18～24 hours, obtain the bacillus subtilis seed liquor in 28～31 ℃ of vibrations;
(4) inoculated and cultured:
The volume ratio of above-mentioned seed liquor by 1-3% is inoculated in the culture medium, cultivated 18～24 hours, measure bacterial count of bacillus subtilis and be not less than 1,000,000,000/ml in 28～31 ℃ of vibrations;
(5) collect bacterial classification:
Behind the no living contaminants of pure bacterium test, the centrifugal supernatant of abandoning is collected thalline then with above-mentioned culture.
5, the cultivation of Bifidobacterium
Its cultural method comprises the steps:
(1) dull and stereotyped cultivation rejuvenation: the freeze-drying lactobacillus of Bifidobacterium is dissolved with calf serum, be inoculated on the solid medium, cultivated 72 hours down, make the Bifidobacterium rejuvenation, choose the single bacterium colony on the plate, carry out morphology and CHARACTERISTICS IDENTIFICATION in 36 ± 1 ℃;
(2) culture medium preparation:
In w/v; Beef extract 3%, sodium chloride 5%, distilled water 1000ml are boiled; With many peptone 10%, plant peptone 10%, yeast extract 5%, pancreatin hydrolysis thing 3%, glucose 10%, soluble starch 0.5%, L-cysteine 0.5%, Tween-80 1ml, vitamin K 0.11%, hemin 0.005%, potassium dihydrogen phosphate 1%, magnesium sulfate 0.2%, ferrous sulfate 0.01%, manganese sulfate 0.00674%, sodium chloride 0.01% and mix; Transfer pH to 7.2,121 ℃ of sterilizations of high pressure 20 minutes, subsequent use;
(3) preparation of seed liquor:
Bifidobacterium colonies typical in the step (1) is inoculated in the above-mentioned culture medium, cultivated 18～24 hours, obtain the seed liquor of Bifidobacterium in 28～31 ℃ of vibrations;
(4) inoculated and cultured:
The volume ratio of above-mentioned seed liquor by 1-3% is inoculated in the culture medium, cultivated 18～24 hours, measure Bifidobacterium bacterium number and be not less than 1,000,000,000/ml in 37～41 ℃ of anaerobism;
(5) collect bacterial classification:
Behind the no living contaminants of pure bacterium test, the centrifugal supernatant of abandoning is collected thalline then, adds 30% glycerol liquor, preserves down at-40 ℃ with above-mentioned culture.
The characteristics of microorganism high activity preparation of the present invention:
The present invention adopts photosynthetic bacteria, Bacillus acidi lactici, S. cervisiae, bacillus subtilis, Bifidobacterium biology characteristics and the probio class of function separately to carry out compatibility.
Wherein, photosynthetic bacteria is a kind of nutritious, bacterium of being of high nutritive value, and thalline contains rich in amino acid, vitamin and biotin, also has the physiological activator ubiquinone.Photosynthetic bacteria is added in the feed as feed addictive, can promote animal to the digesting and assimilating of feed, and improves efficiency of feed utilization, also can significantly improve the speed of growth of animal simultaneously.
Bacillus acidi lactici is a kind of microorganism of in anaerobism or few oxygen environment, surviving liked; Be the dominant microflora in the normal flora in the animal alimentary canal, can reduce cholesterol, in and enterotoxin; Synthetic nutriment and generation digestive ferment have the function of regulating the raising poultry nutritive metabolism.
S. cervisiae is the oxygen consumption microorganism, and it can not only help animal digestion, can also promote the vigor of former beneficial microbe in the alimentary canal, replenishes the B family vitamin, the effect that its cell wall polysaccharides also has to stimulate animal immunizing power, improve premunition.
Bacillus subtilis belongs to oxygen consumption type microorganism, can produce hydrogen peroxide, bacteriocin; Set up microecological balance, promote the breeding of useful anaerobe, ability ramp behind the entering alimentary canal; Produce protease, lipase, amylase, cellulase isoreactivity material, digestion, absorption and utilization performance regulating and controlling effect to nutriments such as protein, fat, carbohydrate suppress and killing action with harmful microorganism is risen; Good stability in feed processing, storage and alimentary canal; Need not the processing of special protection property, just can survive for a long time, can be in the feed of domestic animals and fowls such as pig, ox, chicken.
Bifidobacterium is an important physical property bacterium in the humans and animals body; Can combine closely with endogenous flora such as gram-positive bacterium, coccuses, form the physiological barrier, resist the invasion and attack of other pathogenic on the intestinal mucosa surface; Keep the microecological balance in the body, the immunity of enhancing body.
Positive beneficial effect of the present invention:
(1) microorganism high activity preparation of the present invention is made up of safe and efficient useful photosynthetic bacteria, Bacillus acidi lactici, S. cervisiae, bacillus subtilis, Bifidobacterium; The object bacteria purity of product is high, clear efficacy, and bacterial classification belongs to " allowing to use feed addictive kind catalogue "; Can directly feed; Safety is green, nuisanceless preparation, can be widely used in livestock and poultry and aquatic products ecologic breeding.
(2) microorganism high activity preparation compatibility of the present invention is reasonable; Take as the leading factor with photosynthetic bacteria and Bacillus acidi lactici; Each quasi-microorganism can be brought into play effect separately in the preparation, interdepends, and forms the relation of coexistence and co-prosperity each other; The stable state that can keep microorganism is beneficial to the global advantage of bringing into play microorganism formulation.
(3) microorganism high activity preparation of the present invention is easy to breeding; Said preparation can synthesize the mortifier to pathogenic entero becterias such as Escherichia coli, salmonella, staphylococcus, clostridium; Help host's the raising with resistance against diseases of growing; Safe in utilization, humans and animals is caused a disease.
(4) high-activity microorganism protein feed of the present invention is compared with normal diet and is had obvious advantage; Normal diet promotes growth of animal through adding antibiotic, hormone and growth accelerator often; Feed of the present invention is to come somatotrophic through adding microorganism high activity preparation; Microorganism high activity preparation can be decomposed into small organic molecule with the larger molecular organics in the feed; Bacillus acidi lactici wherein and bacillus subtilis can be decomposed in the feed indissoluble plant cell wall (cellulose, lignin, pectin etc.) under the normal temperature; High molecular weight protein is degraded to little peptide or oligopeptides material, polysaccharide is degraded to monose or oligosaccharides, help the abundant absorption of animal nutritional labeling.
(5) high-activity microorganism protein feed of the present invention; Through adding microorganism high activity preparation; This feed gets into behind animal intestinal that beneficial bacterium forms powerful dominant population in enteron aisle; Can suppress and eliminate pathogenic flora, can synthesize nutrition class materials such as a large amount of amino acid, protein, various biochemical enzymes, somatomedin simultaneously; The function of ability adjustment and each organ of raising livestock and poultry human body is improved food conversion ratio, and can prevent and cure diseases, and improves survival rate, promote to grow and breeding, and minimizing fecaluria stink, environment purification, the effect of increasing income is obvious.
(6) high-activity microorganism protein feed of the present invention is selected materials rationally, selects the additive that can promote pig growth and development for use; Can improve the immunocompetence of pig, the metabolizable energy value of feed is moderate, be of high nutritive value, good palatability, be prone to digest and assimilate the nutritional need of the pig that is content with very little growth; Use the back piglet or the stage of fattening pig daily gain improve; Premunition strengthens, the death rate reduces, feed coefficient is low, can reduce feeding cost, has excellent popularization and is worth.
(7) after high-activity microorganism protein feed of the present invention adds Chinese medicine additive, alternative interpolation antibiotic etc, it is without any side effects that institute adds Chinese medicine, can strengthen disease resistance of animals.This feed is not only applicable to the nursing of pig, is applicable to the nursing of other livestock and poultry yet, can improve the quality and the local flavor of animal products; Strengthen the international competitiveness of animal products; Help the sustainable and healthy development of animal husbandry, help human health, help environmental protection.Therefore, the present invention has important practical significance.
The specific embodiment
Embodiment 1: microorganism high activity preparation and high-activity microorganism protein feed and preparation method thereof
1, a kind of microorganism high activity preparation by weight percentage, comprises following component, photosynthetic bacteria 15%, Bacillus acidi lactici 35%, S. cervisiae 15%, bacillus subtilis 25%, Bifidobacterium 10%.
Wherein the cultural method of photosynthetic bacteria, Bacillus acidi lactici, S. cervisiae, bacillus subtilis, bifidobacterium species is seen the technical scheme part of specification.
2, a kind of high-activity microorganism protein feed by weight percentage, comprises following component: dried fish 6%, blood meal 10%; Soybean 10%, dregs of beans 40%, peanut meal 10%; DDGS feed 6%, calcium monohydrogen phosphate 7%, salt 1%; Soyabean oligosaccharides 5%, microorganism high activity preparation 3%, vitamin compound premix 2%.
Said vitamin compound premix is: in every kilogram of vitamin compound premix, vitamin A is not less than that 336000IU, vitamin D are not less than 5200 IU, vitamin E is not less than 480IU, vitamin K
3Be not less than 40mg, Cobastab
1Be not less than 54.4mg, Cobastab
2Be not less than 160mg, Cobastab
6Be not less than 120mg, Cobastab
12Being not less than 0.64mg, calcium pantothenate is not less than 320mg, nicotinic acid and is not less than 140mg, folic acid and is not less than 16mg, biotin and is not less than 1.28mg, choline and is not less than 12.5mg, iron and is not less than 1750mg, zinc and is not less than 1750mg, copper and is not less than 112.5mg, manganese and is not less than 75mg, iodine and is not less than 3.5mg, selenium and is not less than 7mg, cobalt and is not less than 5mg, lysine and is not less than that 50g, calcium are not less than 177g, total phosphorus is not less than 36g.
The preparation method of said high-activity microorganism protein feed mixes dried fish blood meal, soybean, behind high temperature puffing, pulverizes; Pour agitator tank then into, dregs of beans, peanut meal, DDGS feed are pulverized, pour in the above-mentioned agitator tank; Under agitation add calcium monohydrogen phosphate, salt, vitamin compound premix, stirred 10-15 minute, add soyabean oligosaccharides; Simultaneously the spraying of microorganism high activity preparation is added, stirred 10-15 minute, carry out vacuum packaging.
Wherein the DDGS feed is the trade name of vinasse protein feed, promptly contains the distiller's dried grain of soluble solids; The protein content of DDGS feed is more than 26%.
Adopt high-activity microorganism protein feed of the present invention can process complete feed, the formulation by weight of complete diet pellet is:
The nursing stage of 15kg-30kg pig: corn 70%, wheat bran 6%, high-activity microorganism protein feed 24%;
The nursing stage of 30kg-60kg pig: corn 70%, wheat bran 12%, high-activity microorganism protein feed 18%;
The nursing stage that the 60kg-pig is delivered for sale: corn 70%, wheat bran 16%, high-activity microorganism protein feed 14%.
Embodiment 2: basic identical with embodiment 1, difference is:
1, a kind of microorganism high activity preparation by weight percentage, comprises following component, photosynthetic bacteria 13%, Bacillus acidi lactici 35%, S. cervisiae 18%, bacillus subtilis 24%, Bifidobacterium 10%.
2, a kind of high-activity microorganism protein feed by weight percentage, comprises following component: dried fish 8%, blood meal 8%; Soybean 12%, dregs of beans 35%, peanut meal 13%; DDGS feed 7.5%, calcium monohydrogen phosphate 5%, salt 1.2%; Soyabean oligosaccharides 4.8%, microorganism high activity preparation 2.5%, vitamin compound premix 2%;
3, the preparation method of high-activity microorganism protein feed is with embodiment 1.
Embodiment 3: basic identical with embodiment 1, difference is:
1, a kind of microorganism high activity preparation by weight percentage, comprises following component, photosynthetic bacteria 15%, Bacillus acidi lactici 30%, S. cervisiae 18%, bacillus subtilis 22%, Bifidobacterium 15%.
2, a kind of high-activity microorganism protein feed by weight percentage, comprises following component: dried fish 8%, blood meal 8%; Soybean 12%, dregs of beans 35%, peanut meal 13%, DDGS feed 8%; Calcium monohydrogen phosphate 5%, salt 1.2%, soyabean oligosaccharides 4.8%; Microorganism high activity preparation 2.5%, vitamin compound premix 2% also has Chinese medicine 0.5%;
Traditional Chinese medicine ingredients is: in weight portion, and 0.625 part of the Chinese bulbul, 0.25 part in Radix Glycyrrhizae, 0.25 part of rheum officinale, 0.65 part of Radix Isatidis; 0.65 part of mother chrysanthemum, 0.4 part of cape jasmine, 0.35 part of betel nut, 0.4 part of Chinese violet; 0.35 part of alum, 0.2 part of realgar, 1.0 parts of rhizome of cyrtomium, 0.008 part of the dried venom of toads.
3, the preparation method of high-activity microorganism protein feed mixes dried fish blood meal, soybean, behind high temperature puffing, pulverizes; Pour agitator tank then into, dregs of beans, peanut meal, DDGS feed are pulverized, pour in the above-mentioned agitator tank; Under agitation add calcium monohydrogen phosphate, salt, vitamin compound premix, stirred 10-15 minute, add soyabean oligosaccharides and Chinese medicine; Simultaneously the spraying of microorganism high activity preparation is added, stirred 10-15 minute, carry out vacuum packaging.
Embodiment 4: microorganism high activity preparation and piglet thereof are with mixed feed and preparation method
1, microorganism high activity preparation by weight percentage, comprising: photosynthetic bacteria 12%, Bacillus acidi lactici 30%, S. cervisiae 18%, bacillus subtilis 22%, Bifidobacterium 18%.Each culture of strains method is seen the technical scheme part of specification.
2, a kind of piglet is used mixed feed, by weight percentage, comprises following component: corn 31.5%; Wheat bran 8.5%, dregs of beans 15.5%, soybean 15%; Whey powder 15%, fish meal 7%, calcium monohydrogen phosphate 0.5%; Soyabean oligosaccharides 5%, microorganism high activity preparation 1%, vitamin compound premix 1%.The composition of vitamin compound premix is with embodiment 1.
3, the preparation method of mixed feed pulverizes corn, wheat bran, dregs of beans, soybean, the expanded back of fish meal, in agitator tank, mixes; Under agitation add calcium monohydrogen phosphate, whey powder, vitamin compound premix; Stirred 10-15 minute, and added soyabean oligosaccharides, simultaneously the spraying of microorganism high activity preparation is added; Stirred 10-15 minute, and carried out vacuum packaging.
Embodiment 5: microorganism high activity preparation and piglet thereof are basic identical with mixed feed and preparation method and embodiment 4, and difference is:
1, microorganism high activity preparation by weight percentage, comprising: photosynthetic bacteria 12%, Bacillus acidi lactici 35%, S. cervisiae 18%, bacillus subtilis 22%, Bifidobacterium 13%.
2, a kind of piglet is used mixed feed, comprises following component: corn 34%, wheat bran 7%, dregs of beans 12%; Soybean 13%, whey powder 17%, fish meal 8%, calcium monohydrogen phosphate 0.5%; Soyabean oligosaccharides 5%, microorganism high activity preparation 2%, vitamin compound premix 1.5%.
3, the preparation method of mixed feed, identical with embodiment 4, no longer repeat.
Embodiment 6: microorganism high activity preparation and piglet thereof are basic identical with mixed feed and preparation method and embodiment 4, and difference is:
1, microorganism high activity preparation by weight percentage, comprising: photosynthetic bacteria 10%, Bacillus acidi lactici 40%, S. cervisiae 20%, bacillus subtilis 20%, Bifidobacterium 10%.
2, a kind of piglet is used mixed feed, comprises following component: corn 38%, wheat bran 5%, dregs of beans 20%, soybean 10%, whey powder 10%, fish meal 5%, calcium monohydrogen phosphate 1%, soyabean oligosaccharides 6%, microorganism high activity preparation 2%, vitamin compound premix 3%.
3, the preparation method of mixed feed, identical with embodiment 4, no longer repeat.
Should be noted that: above embodiment only is used for explanation and also unrestricted the present invention, and embodiment can be a formula combination possible in the scope of claim of the present invention.As long as under the prerequisite that does not break away from the present invention's design, those skilled in the art can carry out the conversion of any routine, all belongs to protection scope of the present invention.
Claims (8)
1. a microorganism high activity preparation is characterized in that: by weight percentage, comprise following component; Photosynthetic bacteria 10%-20%, Bacillus acidi lactici 20%-40%, S. cervisiae 10%-20%; Bacillus subtilis 20%-30%, Bifidobacterium 10%-20%.
2. microorganism high activity preparation as claimed in claim 1 is characterized in that: photosynthetic bacteria 10%-15% wherein, Bacillus acidi lactici 30%-40%, S. cervisiae 15%-20%, bacillus subtilis 20%-25%, Bifidobacterium 10%-20%.
3. a high-activity microorganism protein feed that contains claim 1 or 2 said microorganism high activity preparations is characterized in that: by weight percentage, comprise following component: dried fish 5%-10%, blood meal 8%-15%; Soybean 8%-15%, dregs of beans 35%-45%, peanut meal 8%-15%; DDGS feed 5-10%, calcium monohydrogen phosphate 5%-10%, salt 1-1.5%; Soyabean oligosaccharides 3%-10%, microorganism high activity preparation 1%-3%, vitamin compound premix 1%-3%.
4. high-activity microorganism protein feed as claimed in claim 3; It is characterized in that: said vitamin compound premix is: in every kilogram of vitamin compound premix, vitamin A is not less than that 336000IU, vitamin D are not less than 5200 IU, vitamin E is not less than 480IU, vitamin K
3Be not less than 40mg, Cobastab
1Be not less than 54.4mg, Cobastab
2Be not less than 160mg, Cobastab
6Be not less than 120mg, Cobastab
12Being not less than 0.64mg, calcium pantothenate is not less than 320mg, nicotinic acid and is not less than 140mg, folic acid and is not less than 16mg, biotin and is not less than 1.28mg, choline and is not less than 12.5mg, iron and is not less than 1750mg, zinc and is not less than 1750mg, copper and is not less than 112.5mg, manganese and is not less than 75mg, iodine and is not less than 3.5mg, selenium and is not less than 7mg, cobalt and is not less than 5mg, lysine and is not less than that 50g, calcium are not less than 177g, total phosphorus is not less than 36g.
5. high-activity microorganism protein feed as claimed in claim 4 is characterized in that: also contain Chinese medicine 0.5%-1% in the said feed ingredient, traditional Chinese medicine ingredients is in weight portion, and wherein the Chinese bulbul is 0.625 part; 0.25 part in Radix Glycyrrhizae, 0.25 part of rheum officinale, 0.65 part of Radix Isatidis, 0.65 part of mother chrysanthemum; 0.4 part of cape jasmine, 0.35 part of betel nut, 0.4 part of Chinese violet, 0.35 part of alum; 0.2 part of realgar, 1.0 parts of rhizome of cyrtomium, 0.008 part of the dried venom of toads.
6. the preparation method of each said high-activity microorganism protein feed of claim 3-5 is characterized in that: dried fish, blood meal, soybean are mixed, through high temperature puffing; Pulverize, pour agitator tank then into, dregs of beans, peanut meal, DDGS feed are pulverized; Pour in the above-mentioned agitator tank, under agitation add calcium monohydrogen phosphate, salt, vitamin compound premix, stirred 10-15 minute; Add soyabean oligosaccharides; Simultaneously the spraying of microorganism high activity preparation is added, stirred 10-15 minute, carry out vacuum packaging.
7. a piglet that contains claim 1 or 2 said microorganism high activity preparations is used mixed feed, it is characterized in that: by weight percentage, comprise following component: corn 30%-40%; Wheat bran 5%-10%, dregs of beans 10%-20%, soybean 10%-20%; Whey powder 10%-20%, fish meal 5%-10%, calcium monohydrogen phosphate 0.3%-1%; Soyabean oligosaccharides 3%-10%, microorganism high activity preparation 1%-3%, vitamin compound premix 1%-3%.
8. the described piglet of claim 7 is characterized in that with the preparation method of mixed feed: corn, wheat bran, dregs of beans, soybean, the expanded back of fish meal are pulverized, in agitator tank, mix; Under agitation add calcium monohydrogen phosphate, whey powder, vitamin compound premix; Stirred 10-15 minute, and added soyabean oligosaccharides, simultaneously the spraying of microorganism high activity preparation is added; Stirred 10-15 minute, and carried out vacuum packaging.
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| CN102965319A (en) * | 2012-11-30 | 2013-03-13 | 山东省林业科学研究院 | Microbial agent and bio-organic fertilizer special for winter jujube and preparation methods thereof |
| CN103141708A (en) * | 2013-04-02 | 2013-06-12 | 山东新希望六和集团有限公司 | Feed for improving lean meat factor of fattening pig and preparation method of feed |
| CN105558407A (en) * | 2016-01-08 | 2016-05-11 | 北京海正兴潮生物技术有限公司 | Disease resisting and growth promoting additive for poultry, production method thereof and poultry feed |
| CN105614161A (en) * | 2015-12-27 | 2016-06-01 | 陈洪福 | Feed for fishes and shrimps and preparation method of feed |
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| CN106333103A (en) * | 2016-08-29 | 2017-01-18 | 广西兴胜农牧有限公司 | Feed additive for local pigs |
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| CN113213642A (en) * | 2021-04-01 | 2021-08-06 | 浙江理工大学 | Water-regulating microecological preparation for micropterus salmoides seedling culture and preparation method thereof |
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| CN102965319B (en) * | 2012-11-30 | 2013-12-11 | 山东省林业科学研究院 | Microbial agent and bio-organic fertilizer special for winter jujube and preparation methods thereof |
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| CN105614161A (en) * | 2015-12-27 | 2016-06-01 | 陈洪福 | Feed for fishes and shrimps and preparation method of feed |
| CN105558407A (en) * | 2016-01-08 | 2016-05-11 | 北京海正兴潮生物技术有限公司 | Disease resisting and growth promoting additive for poultry, production method thereof and poultry feed |
| CN105995108A (en) * | 2016-07-04 | 2016-10-12 | 安徽省正大源饲料集团有限公司 | Green pig feed |
| CN106333103A (en) * | 2016-08-29 | 2017-01-18 | 广西兴胜农牧有限公司 | Feed additive for local pigs |
| CN106819468A (en) * | 2017-01-03 | 2017-06-13 | 广西聚银牧业集团有限公司 | A kind of pig starter feed and preparation method thereof |
| CN110777185A (en) * | 2019-11-07 | 2020-02-11 | 张宏 | Plant wall-breaking agent stock solution and preparation method and application thereof |
| CN110777185B (en) * | 2019-11-07 | 2021-07-27 | 张宏 | Plant wall-breaking agent stock solution and preparation method and application thereof |
| CN111802514A (en) * | 2020-07-07 | 2020-10-23 | 广东理想生物科学研究有限公司 | Microbial modifier and preparation method and application thereof |
| CN113213642A (en) * | 2021-04-01 | 2021-08-06 | 浙江理工大学 | Water-regulating microecological preparation for micropterus salmoides seedling culture and preparation method thereof |
| CN114886021A (en) * | 2022-03-22 | 2022-08-12 | 贵州大学 | Angus beef cattle feed and preparation method thereof |
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