CN106689672A - Synbiotics composition, synbiotics preparation, preparation method and application thereof - Google Patents
Synbiotics composition, synbiotics preparation, preparation method and application thereof Download PDFInfo
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- CN106689672A CN106689672A CN201710006183.1A CN201710006183A CN106689672A CN 106689672 A CN106689672 A CN 106689672A CN 201710006183 A CN201710006183 A CN 201710006183A CN 106689672 A CN106689672 A CN 106689672A
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Abstract
The invention discloses a synbiotics composition, a synbiotics preparation, a preparation method and application thereof and relates to the technical field of microorganism additives. The synbiotics preparation prepared from the synbiotics composition is prepared from the following components in parts by weight: 400-500 parts of rice bran, 5-15 parts of lactic acid bacteria, 2-8 parts of enterococcus faecalis, 2-8 parts of enterococcus faecium, 160-240 parts of clostridium butyricum, 5-15 parts of bacillus subtilis, 290-340 parts of isomalto-oligosaccharides and 0-5 parts of a mildew preventive. According to the synbiotics composition, the technical problems of feeding synbiotics produced at present that the bacterium content is low, the content of contaminating microorganisms is high and the quality is not stable are solved; the synbiotics composition and the synbiotics preparation, disclosed by the invention, are cooperatively matched with a plurality of types of probiotics and prebiotics, and a gastrointestinal environment capable of maintaining body health is established and maintained; the growth speed of livestock and poultry is improved and the immunity of the organism is enhanced; and the diarrhea rate and the morbidity are reduced, and the production performance of the livestock and poultry is improved.
Description
Technical field
The present invention relates to microbe additive technical field, in particular to a kind of symphysis unit composition, symphysis unit system
Agent, preparation method and applications.
Background technology
Symphysis unit (synbiotics) is also called Synbiotics, refers to the combination preparation of probiotics and prebiotics, or adds
Vitamin, trace element etc..Probiotics is by absorbing appropriate amount, can play useful effect to the healthy of eater
Viable bacteria；Prebiotics refers to can be with the breeding of profitable strain being colonized in selective stimulating enteron aisle or a kind of meal supplement of activity
Agent, it can neither be utilized for livestock and poultry own absorption, can not be again most of harmful bacteria utilization in enteron aisle, and be only most of benefit
Raw bacterium utilizes, so that beneficial bacterium hyperplasia.
Symphysis unit, by probiotics and prebiotics use in conjunction, can simultaneously play prebiotic as microecologic regulator of new generation
Bacterium and the physiological function of prebiotics, make probiotics and prebiotics coordinative role, jointly to anti-disease, safeguard that the Tiny ecosystem of body is put down
Weighing apparatus, can both play the physiological bacterial activity of probiotics, and optionally quick increase probiotics quantity, make probiotic action
Significantly persistently.For simple probiotics or prebiotics, symphysis unit has more preferable cost performance, and market potential is bigger.
Current symphysis unit has been applied to field of fodder, because current animal feed is generally using steam regulation of texture, conditioning of texture granulation skill
Art, the hot and humid condition of the process can kill a large amount of heat labile microorganisms, therefore symphysis unit is used as feed addictive, its
In many viable bacterias not resistant against high temperatures and inactivate so that the effect of probiotics declines, it is impossible to play its effect.In addition it is raw at present
Also there is the shortcomings of bacteria containing amount is low, and miscellaneous bacteria rate is high, quality is unstable in the feeding symphysis unit produced, seriously constrain the feeding micro- life of China
The development and application of state preparation.
In view of this, it is special to propose the present invention.
The content of the invention
The first object of the present invention is to provide a kind of symphysis unit composition, and said composition includes various probiotics and sugar,
By cooperating, the effect of probiotics can be given full play to.
The second object of the present invention is to provide a kind of symphysis unit preparation, and said preparation includes described symphysis unit composition,
Using rice bran as pharmaceutical base, by rational proportion, intestinal microecology can be maintained to balance.
Third object of the present invention is to provide a kind of preparation method of described symphysis unit preparation, the method operation letter
It is single, it is convenient to carry out.
The fourth object of the present invention is to provide a kind of described symphysis unit preparation in pig feed or chicken feed is prepared
Using.
In order to realize above-mentioned purpose of the invention, spy uses following technical scheme：
A kind of symphysis unit composition, the symphysis unit composition is included：Lactic acid bacteria, enterococcus faecalis, VREF, butyric acid shuttle
Bacterium, bacillus subtilis and isomaltulose；
The gross weight of lactic acid bacteria, enterococcus faecalis, VREF, clostridium butyricum and bacillus subtilis and isomaltulose
Weight ratio is 1：(1~2).
A kind of symphysis unit preparation being made according to above-mentioned symphysis unit composition, comprising following component in parts by weight：
400~500 parts of rice bran, 5~15 parts of lactic acid bacteria, 2~8 parts of enterococcus faecalis, 2~8 parts of VREF, clostridium butyricum 160~240
0~5 part of part, 5~15 parts of bacillus subtilis, 290~340 parts of isomaltulose and mould inhibitor.
Preferably, on the basis of the technical scheme that the present invention is provided, above-mentioned symphysis unit preparation is included in parts by weight
Following component：440~480 parts of rice bran, 8~12 parts of lactic acid bacteria, 3~7 parts of enterococcus faecalis, 3~7 parts of VREF, clostridium butyricum
1~3 part of 180~220 parts, 8~12 parts of bacillus subtilis, 290~340 parts of isomaltulose and mould inhibitor.
Preferably, on the basis of the technical scheme that the present invention is provided, above-mentioned symphysis unit preparation is included in parts by weight
Following component：450~470 parts of rice bran, 8~12 parts of lactic acid bacteria, 3~6 parts of enterococcus faecalis, 3~6 parts of VREF, clostridium butyricum
180~200 parts, 8~12 parts of bacillus subtilis, 300~320 parts of isomaltulose and optionally 1~3 part of mould inhibitor.
Preferably, on the basis of the technical scheme that the present invention is provided, above-mentioned symphysis unit preparation is included in parts by weight
Following component：468 parts of rice bran, 10 parts of lactic acid bacteria, 5 parts of enterococcus faecalis, 5 parts of VREF, 200 parts of clostridium butyricum, withered grass gemma
2 parts of 10 parts of bacillus, 300 parts of isomaltulose and mould inhibitor.
Preferably, on the basis of the technical scheme that the present invention is provided, above-mentioned symphysis unit preparation is pulvis, tablet, particle
Agent or capsule.
A kind of preparation method of the symphysis unit preparation described in any of the above-described, the method includes：By the rice bran of formula ratio, breast
Sour bacterium, enterococcus faecalis, VREF, clostridium butyricum, bacillus subtilis, isomaltulose and mould inhibitor are well mixed, and obtain
Symphysis unit preparation.
A kind of application of symphysis unit preparation described in any of the above-described in pig feed or chicken feed is prepared.
Preferably, the present invention provide technical scheme on the basis of, above-mentioned symphysis unit preparation in the application of pig feed,
The addition weight of symphysis unit preparation accounts for the 0.5~0.8% of pig feed weight.
Preferably, the present invention provide technical scheme on the basis of, above-mentioned symphysis unit preparation in the application of chicken feed,
The addition weight of symphysis unit preparation accounts for the 0.3~0.5% of chicken feed weight.
Compared with the prior art, the present invention has the advantages that：
(1) the symphysis unit composition and symphysis unit preparation of present invention offer are with lactic acid bacteria, enterococcus faecalis, VREF, fourth
Sour clostridium, bacillus subtilis are probiotics, and with isomaltulose as prebiotics, clostridium butyricum can significantly increase probiotics effect
Really, isomaltulose can promote the propagation of probiotics, suppress the growth of harmful bacteria, by various probiotics and the association of prebiotics
With coordinating, the digestive tract environment of body health is set up and maintained；Each composition is free of poisonous and harmful substance, will not be produced in body
Raw residual.
(2) preparation method of above-mentioned symphysis unit preparation is simple and easy to apply, is mixed when using and machine is passed transorally into feed
In body.
(3) present invention is provided symphysis unit and the symphysis unit preparation that is made from it are as additive application in animal and fowl fodder
In, a good micro-ecological environment can be established for animal intestinal tract, the speed of growth of livestock and poultry is improved, strengthen immunity of organism
Power, reduces diarrhea rate and the incidence of disease, improves livestock and poultry production performance, builds up health.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the present invention.It is unreceipted specific in embodiment
Condition person, the condition advised according to normal condition or manufacturer is carried out.Agents useful for same or the unreceipted production firm person of instrument, are
The conventional products that can be obtained by commercially available purchase.
According to the first aspect of the invention, there is provided a kind of symphysis unit composition, the symphysis unit composition is included：Breast
Sour bacterium, enterococcus faecalis, VREF, clostridium butyricum, bacillus subtilis and isomaltulose；Lactic acid bacteria, enterococcus faecalis, dung intestines
The gross weight of coccus, clostridium butyricum and bacillus subtilis and the weight ratio of isomaltulose are 1：(1~2).
Lactic acid bacteria is that a class can utilize fermentable carbohydrate to produce the common name of the bacterium of a large amount of lactic acid, and lactic acid bacteria is always
Animal sources lactic acid bacteria and Plant Lactobacilli can be divided on source, vegetalitas lactic acid bacteria is traditional from pickles, salted vegetables, thick broad-bean sauce etc.
A separation screening class lactic acid bacteria out in vegetalitas fermented food, including Lactobacillus plantarum, Lactobacillus casei, Lactobacillus brevis
Deng.
Preferably, lactic acid bacteria of the invention is plant lactobacillus.
Enterococcus faecalis (Enterococcus faecalis) is Gram-positive, and hydrogen peroxide negative cocci is people and is moved
One of flora in thing enteron aisle, it can produce natural antibiotics, be conducive to body health, while bacteriocin etc. can also be produced to press down
Fungus matter, suppresses the growth of the pathogens such as Escherichia coli and salmonella, improves intestinal microenvironment, moreover it is possible to suppress to produce urine in enteron aisle
Plain enzyme bacterium and the breeding of spoilage organisms, reduce enteron aisle urease and endotoxic content, make ammonia and endotoxic content in blood
Decline.
VREF (Enterococcus faecium) belongs to enterococcus spp, is present in the enteron aisle of humans and animals, to intestines
The harmful bacteria of system is inhibited.
Clostridium butyricum (Clostridium butyricum) belongs to Bacillaceae, and fusobacterium, Gram-positive has bud
Spore, spore oval, eccentric or secondary end life can resist poor environment, and adjustment intestinal colony balance promotes intestinal beneficial flora to increase
Grow, strengthen immunity.
Bacillus subtilis is one kind of bacillus, and bacterium colony rough surface is opaque, dirty white or slightly yellow, withered grass
Subtilin, polymyxins, nystatin, the gramicidins isoreactivity material produced during bacillus thalli growth, this
A little active materials have obvious inhibitory action to the conditioned pathogen of pathogenic bacteria or autogenous infection.Bacillus subtilis derives from
China General Microbiological culture presevation administrative center, Bacillus subtilis subspecies (Bacillus subtilis
Subsp.subtilis) deposit number：CGMCC 1.3358.
Above-mentioned these strains can be obtained by commercially available mode or from National Collection unit.
Isomaltulose (IMO) is also known as bifurcation oligose, is the monose being combined into α (1 → 6) glycosidic bond by glucose
Number in 2~5 oligosaccharide not waited, because molecular conformation is different, so being different from maltose and referred to as oligoisomaltose.
In the present invention, the gross weight of lactic acid bacteria, enterococcus faecalis, VREF, clostridium butyricum and bacillus subtilis with
The weight ratio of isomaltulose is 1：(1~2).
By the rational proportion between probiotics and prebiotics, the effect of symphysis unit can be well played.
With lactic acid bacteria, enterococcus faecalis, VREF, clostridium butyricum and bacillus subtilis in symphysis unit composition of the invention
Bacterium, using isomaltulose as prebiotics, by the rational proportion between them, significantly increases probiotics as compound probiotic
Effect, while isomaltulose has the effect for promoting proliferation of probiotics and suppressing harmful bacteria growing, by various probiotics and
The coordinated of prebiotics, hence it is evident that improve body intestinal microecology system, sets up and maintains the digestive tract environment of body health, prevents
Only flora imbalance and there is enteric infection；Each composition is free of poisonous and harmful substance, and residual will not be produced in body.
According to the second aspect of the invention, there is provided the symphysis unit preparation that a kind of above-mentioned symphysis unit composition is made,
Comprising following component in parts by weight：400~500 parts of rice bran, 5~15 parts of lactic acid bacteria, 2~8 parts of enterococcus faecalis, VREF
2~8 parts, 160~240 parts of clostridium butyricum, 5~15 parts of bacillus subtilis, 290~340 parts of isomaltulose and mould inhibitor 0~
5 parts.
Based on each component of above-mentioned symphysis unit composition, rice bran is selectively added symphysis unit preparation as matrix in addition
Symphysis unit preparation is obtained after the additives such as mould inhibitor.
Rice bran is the byproduct of Rice producing, has the advantages that nutriment enriches, is distributed wide, cheap, the present invention
Symphysis unit preparation using rice bran as base matter, in the case where not influenceing probiotics and prebiotics to play a role be body mend
Fill nutriment.
In the present invention, the typical but non-limiting content of rice bran be, for example, 400 weight portions, 410 weight portions, 420 weight portions,
430 weight portions, 440 weight portions, 450 weight portions, 460 weight portions, 470 weight portions, 480 weight portions, 490 weight portions or 500 weights
Amount part.
Preferably, rice bran is defatted rice bran.
Defatted rice bran is removed (content because of grease<2%), storage period is more long.
Lactic acid bacteria, enterococcus faecalis in symphysis unit preparation of the present invention, VREF, clostridium butyricum, bacillus subtilis are equal
Using bacterium powder.
Lactic acid bacteria uses plant lactobacillus bacterium powder, and preparation method is to be concentrated plant lactobacillus, obtains concentrate,
Treatment is dried under conditions of vacuum dehydrating at lower temperature to concentrate, plant lactobacillus bacterium powder is obtained, wherein, plant lactobacillus bacterium
In powder living bacteria count be 100,000,000,000/gram.
Preferably, coating treatment, i.e. microencapsulation are carried out to plant lactobacillus bacterium powder, coating plant lactobacillus bacterium is obtained
Powder.
Coating treatment is carried out by bacterium powder, it is possible to resolve bacterium powder produced problem in feed formulation, including：High humidity, system
Steam during grain can be such that dry bacterium brings back to life in advance, and capsule layer can stop contact of the steam with viable bacteria after being coated with, so that its
From the destruction of steam；Hyperpyrexia, microcapsule layer makes the coated dry bacterium can be with heat-resisting 80~90 DEG C of granulation high temperature, at 100 DEG C
Can also be survived the several seconds under environment；Extruding, capsule layer can prevent dry mycelium destroyed in granulation, maintain its integrality.
In the present invention, the typical but non-limiting content of lactic acid bacteria bacterium powder powder is, for example, 5 weight portions, 6 weight portions, 7 weight
Part, 8 weight portions, 9 weight portions, 10 weight portions, 11 weight portions, 12 weight portions, 13 weight portions, 14 weight portions or 15 weight portions.
Enterococcus faecalis uses enterococcus faecalis bacterium powder, and preparation method is to be concentrated Enterococcus faecalis fermentation liquid, is concentrated
Liquid, vacuum dehydrating at lower temperature treatment is carried out under conditions of vacuum dehydrating at lower temperature to concentrate, obtains enterococcus faecalis bacterium powder, wherein,
In enterococcus faecalis bacterium powder living bacteria count be 100,000,000,000/gram.
Preferably, coating treatment, i.e. microencapsulation are carried out to enterococcus faecalis bacterium powder, coating enterococcus faecalis bacterium powder is obtained.
In the present invention, the typical but non-limiting content of enterococcus faecalis bacterium powder is, for example, 2 weight portions, 3 weight portions, 4 weight
Part, 5 weight portions, 6 weight portions, 7 weight portions or 8 weight portions.
VREF uses VREF bacterium powder, and preparation method is to be concentrated VREF zymotic fluid, is concentrated
Liquid, vacuum dehydrating at lower temperature treatment is carried out under conditions of vacuum dehydrating at lower temperature to concentrate, obtains VREF bacterium powder, wherein,
In VREF bacterium powder living bacteria count be 100,000,000,000/gram.
Preferably, coating treatment, i.e. microencapsulation are carried out to VREF bacterium powder, coating VREF bacterium powder is obtained.
In the present invention, the typical but non-limiting content of VREF bacterium powder is, for example, 2 weight portions, 3 weight portions, 4 weight
Part, 5 weight portions, 6 weight portions, 7 weight portions or 8 weight portions.
Clostridium butyricum uses clostridium butyricum bacterium powder, and preparation method is by the strain inoculation fermentation of activation, drying, crushing are made
Bacterium powder, wherein, in clostridium butyricum bacterium powder living bacteria count be 300,000,000/gram.
In the present invention, the typical but non-limiting content of clostridium butyricum bacterium powder be, for example, 160 weight portions, 170 weight portions,
180 weight portions, 190 weight portions, 200 weight portions, 210 weight portions, 220 weight portions, 230 weight portions or 240 weight portions.
Bacillus subtilis use bacillus subtilis bacterium powder, preparation method be will activate strain inoculation fermentation, drying,
Crushing is made bacterium powder, wherein, in bacillus subtilis bacterium powder living bacteria count be 100,000,000,000/gram.
In the present invention, the typical but non-limiting content of bacillus subtilis bacterium powder is, for example, 5 weight portions, 6 weight portions, 7
Weight portion, 8 weight portions, 9 weight portions, 10 weight portions, 11 weight portions, 12 weight portions, 13 weight portions, 14 weight portions or 15 weight
Part.
In the present invention, the purity of isomaltulose is 90%.Using isomaltulose as prebiotics, can be with above-mentioned many
Probiotics coordinated is planted, improves and maintain body intestinal microecology balance, meanwhile, there is isomaltulose promotion probiotics to increase
Grow and suppress the effect of harmful bacteria growing.
The typical but non-limiting content of isomaltulose is, for example, 290 weight portions, 300 weight portions, 310 weight portions, 320
Weight portion, 330 weight portions or 340 weight portions.
Midew preventive for feed is the generation for suppressing mycotoxin, and the feed for preventing feed moldy metamorphism and extending period of storage adds
Plus agent.Mould inhibitor includes folk prescription mould inhibitor and compound mould inhibitor, and folk prescription mould inhibitor includes propionate compound, formic acid and calcium formate, mountain
Pears acid, citric acid, horse dimethyl phthalate and allicin etc..Two or more different mould inhibitor compatibility is combined
It is compound mould inhibitor, gram mould despot that for example is produced from the U.S..
The typical but non-limiting content of mould inhibitor is, for example, 0 weight portion, 1 weight portion, 2 weight portions, 3 weight portions, 4 weight
Part or 5 weight portions.
The symphysis unit preparation being made up of above-mentioned symphysis unit composition that the present invention is provided with rice bran as pharmaceutical base, by group
/ rational proportion, the effect of symphysis unit can be given full play to, be made that period of storage after preparation is long, convenient use, reach
Maintain the purpose of the good micro-ecological environment of animal intestinal tract.
" mainly by " of the present invention, it is intended that it can also include other components, these other groups in addition to the component
Part assigns the symphysis unit preparation different characteristics.In addition, " mainly by " of the present invention, may be replaced by closing
" being " of formula or " by ... constitute ".
In a preferred embodiment, above-mentioned symphysis unit preparation includes following component in parts by weight：Rice bran 440
~480 parts, 8~12 parts of lactic acid bacteria, 3~7 parts of enterococcus faecalis, 3~7 parts of VREF, 180~220 parts of clostridium butyricum, withered grass bud
1~3 part of 8~12 parts of spore bacillus, 290~340 parts of isomaltulose and mould inhibitor.
In a preferred embodiment, above-mentioned symphysis unit preparation includes following component in parts by weight：Rice bran 450
~470 parts, 8~12 parts of lactic acid bacteria, 3~6 parts of enterococcus faecalis, 3~6 parts of VREF, 180~200 parts of clostridium butyricum, withered grass bud
1~3 part of 8~12 parts of spore bacillus, 300~320 parts of isomaltulose and mould inhibitor.
In a preferred embodiment, above-mentioned symphysis unit preparation includes following component in parts by weight：Rice bran 468
Part, 10 parts of lactic acid bacteria, 5 parts of enterococcus faecalis, 5 parts of VREF, 200 parts of clostridium butyricum, 10 parts of bacillus subtilis, different malt are few
2 parts of 300 parts of sugar and mould inhibitor.
By further optimizing the proportion relation of each component, the effect of symphysis unit preparation can be further improved.
In a preferred embodiment, above-mentioned symphysis unit preparation is pulvis, tablet, granule or capsule.
Symphysis unit preparation is made pulvis, tablet, granule or capsule, it is easy to use, absorbed beneficial to body.
According to the third aspect of the present invention, there is provided a kind of preparation method of described symphysis unit preparation, the method bag
Include：By the rice bran of formula ratio, lactic acid bacteria, enterococcus faecalis, VREF, clostridium butyricum, bacillus subtilis, isomaltulose and
Optionally mould inhibitor is well mixed, and obtains symphysis unit preparation.
The method is by lactic acid bacteria, enterococcus faecalis, VREF, clostridium butyricum, the bacterium powder of bacillus subtilis and different malt
It is mixed into rice bran after oligosaccharides mixing, is optionally added into mould inhibitor, be made the preparation of powder, sheet, particulate form or capsule state, is operated
Simply, material is readily available.
According to the fourth aspect of the present invention, there is provided a kind of described symphysis unit preparation is in pig feed or chicken feed is prepared
Application.
Symphysis unit preparation is applied in pig feed and chicken feed as feed addictive, with the micro- life for improving animal and bird intestines
State environment, reduces the incidence of disease of the intestines problems such as diarrhoea, improves immunity of livestock, promotes the effect of growth of animals or poultry.
In a preferred embodiment, above-mentioned symphysis unit preparation is in the application of pig feed, and symphysis unit preparation adds
Add weight accounts for the 0.5~0.8% of pig feed weight.
In a preferred embodiment, above-mentioned symphysis unit preparation is in the application of chicken feed, and symphysis unit preparation adds
Add weight accounts for the 0.3~0.5% of chicken feed weight.
In vivo studies shows, symphysis unit can promote the growth of piglet and chick as feed addictive, enhance immunity,
Reduce its death rate and the incidence of disease.
In order to further appreciate that the present invention and its effect, with reference to specific effect example, embodiment and comparative example to this hair
It is bright to be further described in detail.
The isomaltulose of effect example 1 is given birth to lactic acid bacteria, enterococcus faecalis, VREF, clostridium butyricum and bacillus subtilis
Influence experiment long
Culture medium：Peptone 10g, beef extract 10g, yeast extract 5g, K2HPO42g, sodium acetate 5g, dibasic ammonium citrate
2g, glucose 20g, MgSO4·7H2O 0.58g、MnSO4·4H2O 0.25g, water 1000mL, adjust pH value to 7.3；
The lactic acid bacteria of activation, enterococcus faecalis, VREF, clostridium butyricum and bacillus subtilis or bacterium powder of working are distinguished
It is inoculated with or puts into the culture medium containing the isomaltulose that percentage by weight is 0.1%, 0.2%, 0.3%, 0.5%, with
Culture medium without isomaltulose is control, puts 37 DEG C of constant-temperatureanaerobic anaerobic shaken cultivation 24h, then with 10 times of dilution plate drops kind
Method determines the concentration of bacterium solution, and measurement result is shown in Table 1.
The isomaltulose of table 1 influences result of the test to each growth of probiotics
As can be seen from Table 1, the isomaltulose of various concentrations is to lactic acid bacteria, enterococcus faecalis, VREF, clostridium butyricum
There is somatotrophic effect with bacillus subtilis, but growth-promoting effect of the concentration at 0.3% or so is optimal.If concentration
Continue to increase, can Developing restraint.It is computed drawing, when lactic acid bacteria, enterococcus faecalis, VREF, clostridium butyricum and withered grass gemma
The gross weight of bacillus and the weight proportion of isomaltulose are 1:When 1.3, isomaltulose to the growth-promoting effect of probiotics most
It is good.
Influence experiment of the isomaltulose of effect example 2 to Escherichia coli and Salmonella growth
The broth medium containing isomaltulose is determined to Escherichia coli and sramana in the method same with effect example 1
The growth effect of Salmonella, the results are shown in Table shown in 2.
The isomaltulose of table 2 is on Escherichia coli and Salmonella growth influence result of the test
From table 2 it can be seen that the isomaltulose of various concentrations has different degrees of to Escherichia coli and salmonella
The effect of Developing restraint, and inhibition level is relevant with the addition of isomaltulose, concentration is higher, and inhibitory action is stronger.
As can be seen here, in symphysis unit composition of the invention with lactic acid bacteria, enterococcus faecalis, VREF, clostridium butyricum and
Bacillus subtilis as compound probiotic, using isomaltulose as prebiotics, by the rational proportion between them, can be with
The propagation of probiotics is remarkably promoted, while isomaltulose also has the effect for suppressing harmful bacteria growing.
Embodiment 1
A kind of symphysis unit preparation, comprises the following steps：
By the coating plant lactobacillus bacterium powder of 10 weight portions, the bag for being coated with enterococcus faecalis bacterium powder, 5 weight portions of 5 weight portions
By VREF bacterium powder, the clostridium butyricum bacterium powder of 200 weight portions, the bacillus subtilis bacterium powder of 10 weight portions and 300 weight portions
Isomaltulose be added in the defatted rice bran of 468 weight portions, add 2 weight portion mould inhibitors, be well mixed, obtain symphysis
First preparation.
Embodiment 2
A kind of symphysis unit preparation, comprises the following steps：
By the coating plant lactobacillus bacterium powder of 5 weight portions, the coating enterococcus faecalis bacterium powder of 8 weight portions, the coating of 2 weight portions
VREF bacterium powder, the clostridium butyricum bacterium powder of 240 weight portions, the bacillus subtilis bacterium powder of 5 weight portions and 340 weight portions it is different
Fructus Hordei Germinatus oligose is added in the defatted rice bran of 500 weight portions, adds 5 weight portion mould inhibitors, is well mixed, and obtains symphysis unit system
Agent.
Embodiment 3
A kind of symphysis unit preparation, comprises the following steps：
By the coating plant lactobacillus bacterium powder of 15 weight portions, the bag for being coated with enterococcus faecalis bacterium powder, 8 weight portions of 2 weight portions
By VREF bacterium powder, the clostridium butyricum bacterium powder of 160 weight portions, the bacillus subtilis bacterium powder of 15 weight portions and 290 weight portions
Isomaltulose be added in the defatted rice bran of 400 weight portions, be well mixed, obtain symphysis unit preparation.
Embodiment 4
A kind of symphysis unit preparation, comprises the following steps：
By the coating plant lactobacillus bacterium powder of 8 weight portions, the coating enterococcus faecalis bacterium powder of 7 weight portions, the coating of 3 weight portions
VREF bacterium powder, the clostridium butyricum bacterium powder of 220 weight portions, the bacillus subtilis bacterium powder of 8 weight portions and 320 weight portions it is different
Fructus Hordei Germinatus oligose is added in the defatted rice bran of 450 weight portions, adds 3 weight portion mould inhibitors, is well mixed, and obtains symphysis unit system
Agent.
Embodiment 5
A kind of symphysis unit preparation, comprises the following steps：
By the coating plant lactobacillus bacterium powder of 12 weight portions, the bag for being coated with enterococcus faecalis bacterium powder, 7 weight portions of 3 weight portions
By VREF bacterium powder, the clostridium butyricum bacterium powder of 180 weight portions, the bacillus subtilis bacterium powder of 12 weight portions and 310 weight portions
Isomaltulose be added in the defatted rice bran of 480 weight portions, add 4 weight portion mould inhibitors, be well mixed, obtain symphysis
First preparation.
Embodiment 6
A kind of symphysis unit preparation, comprises the following steps：
By the coating plant lactobacillus bacterium powder of 9 weight portions, the coating enterococcus faecalis bacterium powder of 4 weight portions, the coating of 6 weight portions
VREF bacterium powder, the clostridium butyricum bacterium powder of 190 weight portions, the bacillus subtilis bacterium powder of 13 weight portions and 310 weight portions
Isomaltulose is added in the defatted rice bran of 440 weight portions, adds 2 weight portion mould inhibitors, is well mixed, and obtains symphysis unit
Preparation.
Comparative example 1
A kind of symphysis unit preparation, comprises the following steps：
By the coating plant lactobacillus bacterium powder of 10 weight portions, the bag for being coated with enterococcus faecalis bacterium powder, 5 weight portions of 5 weight portions
468 weights are added to by VREF bacterium powder, the clostridium butyricum bacterium powder of 200 weight portions, the bacillus subtilis bacterium powder of 10 weight portions
Measure in the defatted rice bran of part, add 2 weight portion mould inhibitors, be well mixed, obtain symphysis unit preparation.
Comparative example 2
A kind of symphysis unit preparation, comprises the following steps：
By the coating enterococcus faecalis bacterium powder of 8 weight portions, the butyric acid for being coated with VREF bacterium powder, 240 weight portions of 2 weight portions
The isomaltulose of clostridium bacterium powder, the bacillus subtilis bacterium powder of 5 weight portions and 340 weight portions is added to the de- of 500 weight portions
In fat rice bran, 5 weight portion mould inhibitors are added, be well mixed, obtain symphysis unit preparation.
Comparative example 3
A kind of symphysis unit preparation, comprises the following steps：
By the coating plant lactobacillus bacterium powder of 15 weight portions, the coating VREF bacterium powder of 8 weight portions, 160 weight portions
The isomaltulose of clostridium butyricum bacterium powder, the bacillus subtilis bacterium powder of 15 weight portions and 290 weight portions is added to 400 weight portions
Defatted rice bran in, be well mixed, obtain symphysis unit preparation.
Comparative example 4
A kind of symphysis unit preparation, comprises the following steps：
By the coating plant lactobacillus bacterium powder of 8 weight portions, the coating enterococcus faecalis bacterium powder of 7 weight portions, the fourth of 220 weight portions
The isomaltulose of sour clostridium bacterium powder, the bacillus subtilis bacterium powder of 8 weight portions and 320 weight portions is added to 450 weight portions
In defatted rice bran, 3 weight portion mould inhibitors are added, be well mixed, obtain symphysis unit preparation.
Comparative example 5
A kind of symphysis unit preparation, comprises the following steps：
By the coating plant lactobacillus bacterium powder of 12 weight portions, the bag for being coated with enterococcus faecalis bacterium powder, 7 weight portions of 3 weight portions
480 weight are added to by the isomaltulose of VREF bacterium powder, the bacillus subtilis bacterium powder of 12 weight portions and 310 weight portions
In the defatted rice bran of part, 4 weight portion mould inhibitors are added, be well mixed, obtain symphysis unit preparation.
Comparative example 6
A kind of symphysis unit preparation, comprises the following steps：
By the coating plant lactobacillus bacterium powder of 9 weight portions, the coating enterococcus faecalis bacterium powder of 4 weight portions, the coating of 6 weight portions
The isomaltulose of VREF bacterium powder, the clostridium butyricum bacterium powder of 190 weight portions and 310 weight portions is added to 440 weight portions
In defatted rice bran, 2 weight portion mould inhibitors are added, be well mixed, obtain symphysis unit preparation.
Comparative example 7
A kind of symphysis unit preparation, comprises the following steps：
By the coating plant lactobacillus bacterium powder of 10 weight portions, the bag for being coated with enterococcus faecalis bacterium powder, 5 weight portions of 5 weight portions
By VREF bacterium powder, the clostridium butyricum bacterium powder of 200 weight portions, the bacillus subtilis bacterium powder of 10 weight portions and 200 weight portions
Isomaltulose be added in the defatted rice bran of 468 weight portions, add 2 weight portion mould inhibitors, be well mixed, obtain symphysis
First preparation.
Comparative example 8
A kind of symphysis unit preparation, comprises the following steps：
By the coating plant lactobacillus bacterium powder of 2 weight portions, the bag for being coated with enterococcus faecalis bacterium powder, 10 weight portions of 10 weight portions
By VREF bacterium powder, the clostridium butyricum bacterium powder of 100 weight portions, the bacillus subtilis bacterium powder of 2 weight portions and 350 weight portions
Isomaltulose is added in the defatted rice bran of 468 weight portions, adds 2 weight portion mould inhibitors, is well mixed, and obtains symphysis unit
Preparation.
The symphysis unit preparation prepared with above-described embodiment 1-6 and comparative example 1-8 carries out in vivo studies to pig and chicken.
Influence of the symphysis unit preparation of effect example 3 to piglet growth performance
1st, experimental animal
The weanling pig 300 that this experiment is fed from pig farm, male and female half and half is randomly divided into 15 groups, every group 20, each group
Weight differences not significantly (P>0.05), with comparativity.
2nd, test method
First group of symphysis unit preparation prepared using embodiment 1 is added in pig starter feed to piglet as feed addictive
Raised, second group of symphysis unit preparation prepared using embodiment 2 is added in pig starter feed to son as feed addictive
Pig is raised, and it is right in pig starter feed that the 3rd group of symphysis unit preparation prepared using embodiment 3 is added to as feed addictive
Piglet is raised, and the 4th group of symphysis unit preparation prepared using embodiment 4 is added in pig starter feed as feed addictive
Piglet is raised, the 5th group of symphysis unit preparation prepared using embodiment 5 is added to pig starter feed as feed addictive
In piglet is raised, the 6th group using embodiment 6 prepare symphysis unit preparation as feed addictive be added to piglet raise
Piglet is raised in material, the 7th group of symphysis unit preparation prepared using comparative example 1 is added to piglet as feed addictive
Piglet is raised in feed, the 8th group of symphysis unit preparation prepared using comparative example 2 is added to son as feed addictive
Piglet is raised in pig feed, the 9th group of symphysis unit preparation prepared using comparative example 3 is added to as feed addictive
Piglet is raised in pig starter feed, the tenth group of symphysis unit preparation prepared using comparative example 4 is added as feed addictive
Piglet is raised in pig starter feed, the 11st group of symphysis unit preparation prepared using comparative example 5 is used as feed addictive
It is added in pig starter feed and piglet is raised, the 12nd group of symphysis unit preparation prepared using comparative example 6 is added as feed
Plus agent be added to pig starter feed in piglet is raised, the 13rd group using comparative example 7 prepare symphysis unit preparation as feeding
Feed additives are raised in being added to pig starter feed to piglet, and the 14th group of symphysis unit preparation prepared using comparative example 8 is made
Piglet is raised for feed addictive is added in pig starter feed, the 15th group of use is not added with symphysis unit preparation of the present invention
Pig starter feed piglet is raised.Wherein, the pig starter feed that each group is used is identical.Symphysis unit in first group to the 14th group
Preparation is added with accounting for the 0.5% of pig starter feed weight amount.
Method for breeding and rearing conditions in experiment are carried out in the usual way, piglet are raised to 60 ages in days, in feeding process
In, observation piglet health condition, records morbidity number, death toll daily.Weighed for every group before experiment, weighed (before weighing in 60 ages in days
Empty stomach 4h), obtain every group of average weight of piglet.
Computational methods are as follows：Survival rate=(death toll during 20- raisings)/20 X 100%；
Enteron aisle morbidity number/20 X 100% during the incidence of disease=raising；
60 age in days average weight=60 age in days piglet TBW/20
3rd, result of the test
Influence result of the symphysis unit preparation to piglet growth performance is as shown in table 3 below.
Influence result of the symphysis unit preparation of table 3 to piglet growth performance
From table 3 it can be seen that by the raising of about two months, with the addition of the feed raising of symphysis unit preparation of the present invention
First group to the 6th group of the piglet death rate and the incidence of disease is not added with the feeding of symphysis unit preparation of the present invention significantly lower than the 15th group
Expect the death rate and the incidence of disease of the piglet of raising.Under normal circumstances, the average weight of 60 age in days piglets is in 20kg or so, first group
There is different degrees of increase to the 6th group of piglet average weight, increase scope in 2.3~3.6kg.It can be seen that, symphysis unit of the present invention
It is added in pig starter feed as feed addictive and uses, the morbidity and mortality of piglet can be reduced, strengthen immunity is carried
The speed of growth and quality of piglet high.
7th group compared with first group, lacks isomaltulose；8th group to the 12nd group respectively with second group to the 6th
Group is compared, and lacks a certain bacterium, and the diarrhea rate of piglet, morbidity and mortality have slightly rising, to the growth promoting function of piglet
Decline, there is no first group to the 6th group of effect.
13rd group compared with first group, isomaltulose is otherwise varied with the weight proportion of compound probiotic, the 14th
Compared with first group, the weight range proportioning of each bacterium is otherwise varied in compound probiotic, all can not well play symphysis for group
First diseases prevention, somatotrophic effect.
Influence of the symphysis unit preparation of effect example 4 to broiler growth performance
1st, experimental animal
This experiment has just gone out the Broiler chicks 600 of shell from chicken house, and male and female half and half is randomly divided into 15 groups, every group 40, each group
Weight differences not significantly (P>0.05), with comparativity.
2nd, test method
First group of symphysis unit preparation prepared using embodiment 1 is added in Broiler chicks feed to meat as feed addictive
Chick is raised, and second group of symphysis unit preparation prepared using embodiment 2 is added to Broiler chicks feed as feed addictive
In Broiler chicks are raised, it is young that the 3rd group of symphysis unit preparation prepared using embodiment 3 is added to meat as feed addictive
Broiler chicks are raised in chicken feed, the 4th group of symphysis unit preparation prepared using embodiment 4 is added as feed addictive
Broiler chicks are raised in Broiler chicks feed, the 5th group of symphysis unit preparation prepared using embodiment 5 is added as feed
Agent is raised in being added to Broiler chicks feed to Broiler chicks, and the 6th group of symphysis unit preparation prepared using embodiment 6 is used as feeding
Feed additives are raised in being added to Broiler chicks feed to Broiler chicks, the 7th group of symphysis unit preparation prepared using comparative example 1
It is added in Broiler chicks feed as feed addictive and Broiler chicks is raised, the 8th group of symphysis prepared using comparative example 2
First preparation is added in Broiler chicks feed as feed addictive and Broiler chicks is raised, and the 9th group is prepared using comparative example 3
Symphysis unit preparation be added in Broiler chicks feed as feed addictive Broiler chicks raised, the tenth group uses comparative example
The 4 symphysis unit preparations for preparing are added in Broiler chicks feed as feed addictive is raised to Broiler chicks, and the 11st group is adopted
The symphysis unit preparation prepared with comparative example 5 is added in Broiler chicks feed as feed addictive raises to Broiler chicks, the
12 groups of symphysis unit preparations prepared using comparative example 6 are added in Broiler chicks feed as feed addictive and Broiler chicks are carried out
Raise, the 13rd group of symphysis unit preparation prepared using comparative example 7 is added in Broiler chicks feed to meat as feed addictive
Chick is raised, and the 14th group of symphysis unit preparation prepared using comparative example 8 is added to Broiler chicks and raised as feed addictive
Broiler chicks are raised in material, the 15th group is entered using the Broiler chicks feed for being not added with symphysis unit preparation of the present invention to Broiler chicks
Row is raised.Wherein, the Broiler chicks feed that each group is used is identical.Symphysis unit preparation is raised with accounting for Broiler chicks in first group to the 14th group
The amount of the 0.3% of material weight is added.
Method for breeding and rearing conditions in experiment are carried out in the usual way, preceding 24h illumination in 7 days, 23h illumination later, 1h
Dark, relative humidity 55~70%, the initial stage of raising keeps 33 DEG C or so of room temperature, gradually reduces later, and minimum room temperature is maintained at 23
DEG C or so.Weekly with 0.1% bromogeramine band chicken spraying disinfection once, immune programme for children is routinely carried out.Ground is flat to support, and uses
Traditional water fountain free water and feeding., to 28 ages in days, in feeding process, observation Broiler chicks are healthy daily for feeding meat chick
Situation, record morbidity number, death toll.Weighed for every group before experiment, weighed (empty stomach 4h before weighing) in 28 ages in days, obtain every group of meat young
The average weight of chicken.
Computational methods are as follows：Survival rate=(death toll during 20- raisings)/20 X 100%；
Enteron aisle morbidity number/20 X 100% during the incidence of disease=raising；
28 age in days average weight=60 age in days Broiler chicks TBW/20
3rd, result of the test
Influence result of the symphysis unit preparation to broiler growth performance is as shown in table 4 below.
Influence result of the symphysis unit preparation of table 4 to broiler growth performance
From table 4, it can be seen that by the raising of 28 days, with the addition of that the feed of symphysis unit preparation of the present invention raises the
One group to the 6th group of the Broiler chicks death rate and the incidence of disease is not added with the feeding of symphysis unit preparation of the present invention significantly lower than the 15th group
Expect the death rate and the incidence of disease of the Broiler chicks of raising.Under normal circumstances, the average weight of 28 age in days Broiler chicks is in 1300kg or so,
First group to the 6th group of Broiler chicks average weight has different degrees of increase, increases scope in 86~168g.It can be seen that, the present invention
Symphysis unit is added in Broiler chicks feed as feed addictive and uses, and can reduce the morbidity and mortality of Broiler chicks, increases
Strong immunity, improves the speed of growth and quality of Broiler chicks.
7th group compared with first group, lacks isomaltulose；8th group to the 12nd group respectively with second group to the 6th
Group is compared, and lacks a certain bacterium, and the diarrhea rate of Broiler chicks, morbidity and mortality have risen, and the growth promotion to Broiler chicks is made
With decline, first group to the 6th group of effect is there is no.
13rd group compared with first group, isomaltulose is otherwise varied with the weight proportion of compound probiotic, the 14th
Compared with first group, the weight range proportioning of each bacterium is otherwise varied in compound probiotic, all can not well play symphysis for group
First diseases prevention, somatotrophic effect.
In sum, symphysis unit of the invention is used as feed addictive, by various probiotics and the association of prebiotics
With cooperation, hence it is evident that improve body intestinal microecology system, the digestive tract environment of body health is set up and maintained, prevent flora imbalance
And there is enteric infection, morbidity and mortality are reduced, meanwhile, use the growth that can promote livestock and poultry after symphysis unit preparation, body
Weight growth rate accelerates.
Although illustrate and describing the present invention with specific embodiment, but will be appreciated that without departing substantially from of the invention
Many other changes and modification can be made in the case of spirit and scope.It is, therefore, intended that in the following claims
Including belonging to all such changes and modifications in the scope of the invention.
Claims (10)
1. a kind of symphysis unit composition, it is characterised in that the symphysis unit composition is included：Lactic acid bacteria, enterococcus faecalis, dung intestines ball
Bacterium, clostridium butyricum, bacillus subtilis and isomaltulose；
The gross weight of lactic acid bacteria, enterococcus faecalis, VREF, clostridium butyricum and bacillus subtilis and the weight of isomaltulose
The ratio between be 1：(1~2).
2. the symphysis unit preparation that a kind of symphysis unit composition according to described in claim 1 is made, it is characterised in that comprising with weight
The following component of amount part meter：400~500 parts of rice bran, 5~15 parts of lactic acid bacteria, 2~8 parts of enterococcus faecalis, 2~8 parts of VREF,
0~5 part of 160~240 parts of clostridium butyricum, 5~15 parts of bacillus subtilis, 290~340 parts of isomaltulose and mould inhibitor.
3. according to the symphysis unit preparation described in claim 2, it is characterised in that comprising following component in parts by weight：Rice bran
440~480 parts, it is 8~12 parts of lactic acid bacteria, 3~7 parts of enterococcus faecalis, 3~7 parts of VREF, 180~220 parts of clostridium butyricum, withered
1~3 part of 8~12 parts of careless bacillus, 290~340 parts of isomaltulose and mould inhibitor.
4. according to the symphysis unit preparation described in claim 2, it is characterised in that comprising following component in parts by weight：Rice bran
450~470 parts, it is 8~12 parts of lactic acid bacteria, 3~6 parts of enterococcus faecalis, 3~6 parts of VREF, 180~200 parts of clostridium butyricum, withered
1~3 part of 8~12 parts of careless bacillus, 300~320 parts of isomaltulose and mould inhibitor.
5. according to the symphysis unit preparation described in claim 2, it is characterised in that comprising following component in parts by weight：Rice bran
468 parts, 10 parts of lactic acid bacteria, 5 parts of enterococcus faecalis, 5 parts of VREF, 200 parts of clostridium butyricum, 10 parts of bacillus subtilis, different wheat
2 parts of 300 parts of bud oligosaccharides and mould inhibitor.
6. according to the symphysis unit preparation described in claim any one of 2-5, it is characterised in that the symphysis unit preparation be pulvis,
Tablet, granule or capsule.
7. a kind of preparation method of symphysis unit preparation according to described in claim any one of 2-6, it is characterised in that the method bag
Include：By the rice bran of formula ratio, lactic acid bacteria, enterococcus faecalis, VREF, clostridium butyricum, bacillus subtilis, isomaltulose and
Optionally mould inhibitor is well mixed, and obtains symphysis unit preparation.
8. application of a kind of symphysis unit preparation according to described in claim any one of 2-6 in pig feed or chicken feed is prepared.
9. the application according to the symphysis unit preparation described in claim 8 in pig feed is prepared, it is characterised in that the symphysis unit
The addition weight of preparation accounts for the 0.5~0.8% of pig feed weight.
10. the application according to the symphysis unit preparation described in claim 8 in chicken feed is prepared, it is characterised in that the symphysis
The addition weight of first preparation accounts for the 0.3~0.5% of chicken feed weight.
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