CN103461670A - Compound synbiotics preparation and its application - Google Patents
Compound synbiotics preparation and its application Download PDFInfo
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- CN103461670A CN103461670A CN2013100237775A CN201310023777A CN103461670A CN 103461670 A CN103461670 A CN 103461670A CN 2013100237775 A CN2013100237775 A CN 2013100237775A CN 201310023777 A CN201310023777 A CN 201310023777A CN 103461670 A CN103461670 A CN 103461670A
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Abstract
The invention discloses a compound synbiotics preparation and its application. The compound synbiotics preparation includes composite Probiotics and Prebiotics, the composite Probiotics comprise Bacillus subtilis and Bacillus licheniformis, the Prebiotics are anyone of yeast cell wall polysaccharides, mannan oligosaccharides, fructo-oligosaccharides, soybean oligosaccharides, isomalto oligosaccharides, lactosucrose, galactooligosaccharides, sucrose thermal oligosaccharides caramel and oligonucleotides xylose; and the survival rate of spores is higher than 90% after the mixing granulation of the compound synbiotics preparation and a feed, and the total number of live bacteria is 1.0*10<9>-1.0*10<11>CFU/g. By utilizing the cooperative effect of different Probiotics and Prebiotics, the compound synbiotics preparation avoids the disadvantages of single Probiotics, increases the content of the Probiotics, reduces the competitor rate, improves the heat resistance and the storability of the synbiotics, improves the quality of feeding synbiotics, and has a wide application prospect.
Description
Technical field
The present invention relates to the microorganism fodder additive field, especially relate to a kind of compound symphysis unit's preparation and application thereof.
Background technology
Along with the development of aviculture, each state all takes many kinds of measures to purify poultry disease to control.But the problems such as the antibiotic extensively drug residue of food that long-term use causes and microorganism drug resistance, brought huge risk to aquaculture.
Thus, people start the chemical residual of meat, egg food is proposed to strict requirement, and the application of chemical prevention agent in animal-breeding is restricted, and Europe has forbidden using any antibiotic especially in animal diets.Adopt which kind of measure to replace antibiotic application, can not bring above-mentioned risk can ensure the health of animal again, become the Tough questions urgently to be resolved hurrily that aquaculture faces simultaneously.
For this reason, animal microecological educational circles has proposed the theory of " the final using microbe of the problem of microorganism solves ", under the guide of this theory, the technology that the biological competition method is controlled disease has caused scholar's concern, animal microecology (Animal Microecology) has obtained developing rapidly (Tomohiro H, 1983; Xu C L, 2006).Utilize in recent years the principle of " with bacterium, controlling bacterium ", use probio (Probiotics) to prevent that from the microecology angle disease from infecting, obtained the extensive approval of international academic community, become one of focus of current application microbial technique research.
Calendar year 2001, the World Food Programme and the World Health Organization have done as given a definition probio, " by absorbing suitable amount, to eater's the healthy viable bacteria that can bring into play useful effect ".Feeding probio produces bacterial strain and not only requires prebiotic function remarkable, also must can tolerate the high temperature in the feed process, and can low-cost, large scale fermentation cultivation.At present, domestic and international application probio the most widely is lactic acid bacteria class, and the prebiotic function of lactic acid bacteria class is remarkable, but because it can not tolerate the high temperature in the feed process, unstable product quality, application potential and scope are restricted.
Prebiotics (Prebiotics), refer to can the selective stimulating enteron aisle in breeding or active a kind of dietary supplements of the profitable strain of field planting, it can not absorb for livestock and poultry self, it can not be again most of harmful bacteria utilization in enteron aisle, and can only utilize for most of probio, thereby make the beneficial bacterium hyperplasia.
Along with deepening continuously of research, people, gradually from using single culture to transfer the use composite bacteria to, carry out with the non-viable bacteria prebiotics composition with adjusting animal micro-ecological environment the symphysis unit preparation that compound synergic is made from only using active bacteria formulation to transfer the application viable bacteria to.
Symphysis unit (synbiotics) is called again Synbiotics, refers to the combination preparation of probio and prebiotics, or adds vitamin, trace element etc. again.It both can bring into play the physiological bacterial activity of probio, optionally increased fast again the quantity of this bacterium, made probiotic action significantly lasting.Symphysis unit, as microecologic regulator of new generation, by probio and prebiotics use in conjunction, can bring into play the physiological function of probio and prebiotics simultaneously, makes probio and prebiotics coordinative role, jointly resists disease, safeguards the microecological balance of body.Compare simple probio or prebiotics, symphysis unit has better cost performance, and market potential is larger.But, because current animal feed generally adopts the steam regulation of texture, conditioning of texture granulation technique, the hot and humid condition of this process can be killed a large amount of heat labile microorganisms, therefore symphysis unit is as feed addictive, and wherein the resistance to elevated temperatures of viable bacteria is one of bottleneck of restriction probiotics application, in addition, the feeding symphysis unit produced at present also exists bacteria containing amount low, the shortcomings such as the miscellaneous bacteria rate is high, and quality is unstable, seriously restricted development and the application of China's feeding micro-ecological preparation.
Summary of the invention
Main purpose of the present invention is to provide a kind of compound symphysis unit's preparation and application thereof, the first preparation of this compound symphysis utilizes the cooperative effect of different probios and prebiotics, not only avoided the deficiency of single probio, but also increased probio content, reduced the miscellaneous bacteria rate, and improved heat resistance and the storage property of described symphysis unit, improved feeding symphysis unit quality, had broad application prospects.
In order to reach purpose of the present invention, the present invention is by the following technical solutions: a kind of compound symphysis unit preparation, comprise compound probiotic and prebiotics, described compound probiotic comprises bacillus subtilis and bacillus licheniformis, and described prebiotics is any one in yeast cell wall polysaccharide, manna oligosacchride, FOS, soyabean oligosaccharides, isomaltulose, lactosucrose, few lactose, low caramelan and few wood sugar; After symphysis unit's preparation and feed mixing granulation, the gemma survival rate is higher than 90%, and total viable count is 1.0 * 10
9～1.0 * 10
11cFU/g (CFU/g: the total number of bacterial colonies contained in every gram sample).
Preferably, the first preparation of described compound symphysis, by weight percentage, 10～35 parts of compound probiotics, 65～75 parts of prebioticses.
Preferably, in described compound probiotic, the percentage by weight between two kinds of bacterium is: bacillus subtilis 30～55%, bacillus licheniformis 45～70%; After mixing, total viable count is 1.0 * 10
9～1.0 * 10
11cFU/g.
Preferably, the described bacillus subtilis of selecting (Bacillus subtillus) culture presevation number is: CCTCC NO.M209317, and the described bacillus licheniformis of selecting (Bacilluslicheniformis subsp.licheniformis) culture presevation number is: CCTCC NO.M208251; The former powder living spores of bacillus subtilis number is 10,000～2,000,000,000,000/g, the several 5,000～1,000,000,000,000/g of the former powder living spores of bacillus licheniformis; Withered grass, bacillus licheniformis preparation technology adopt respectively applicant Wuhan Kenuo Biology Science & Technology Co., Ltd. from dominant patent: ZL201010147918.0 and ZL200910063822.3.
Preferably, described prebiotics is the yeast cell wall polysaccharide.
Preferably, described yeast cell wall polysaccharide comprises glucan, mannosan, and its quality percentage composition is respectively: Gentran 40～50%, and mannosan content 30～40%, other content are 20～30%.
The application of the first preparation of a kind of compound symphysis, add the first preparation of described compound symphysis in animal drinking water to by mass fraction 0.05%～0.2%.
The application of the first preparation of a kind of compound symphysis is added 0.5～1.5g by the first preparation of described compound symphysis in per kilogram animal basal diet.
The application of a kind of compound symphysis unit preparation, will the first formulation application of described compound symphysis in animal feed straw decomposing process.
Preferably, the animal feed stalk water of pulverizing or cutting off after (3～5cm) is watered, permeates, add 2～5% Diammonium phosphate (DAP) (with the stalk dry weight basis) and mix, the stalk water content is 60～70%.Then in the ratio of 1: 2000, described compound symphysis unit feed addictive is joined in stalk.Play heap, obturage with the particle film.
The invention has the beneficial effects as follows: the compound symphysis of the present invention unit preparation has high-purity, high activity, low miscellaneous bacteria and the advantage such as high temperature resistant.This class symphysis unit is mixed from different animal feeds, can suppress pernicious bacteria survival ability in animal body, strengthen animal immunizing power, reduce the use amounts of chemicals in aquaculture such as antibiotic for animals, hormone, improve meat product safety, and improve the meat of animal, increase the delicate flavour of muscle.
The specific embodiment
Below by embodiment, the present invention is further detailed.
The compound symphysis of embodiment of the present invention unit preparation, comprise compound probiotic and prebiotics, described compound probiotic comprises bacillus subtilis and bacillus licheniformis, and described prebiotics is any one in yeast cell wall polysaccharide, manna oligosacchride, FOS, soyabean oligosaccharides, isomaltulose, lactosucrose, few lactose, low caramelan and few wood sugar; After symphysis unit's preparation and feed mixing granulation, the gemma survival rate is higher than 90%, and total viable count is 1.0 * 10
9～1.0 * 10
11cFU/g.
The first preparation of described compound symphysis, by weight percentage, 10～35 parts of compound probiotics, 65～75 parts of prebioticses.
Percentage by weight in described compound probiotic between two kinds of bacterium is: bacillus subtilis 30～55%, bacillus licheniformis 45～70%.
Described bacillus subtilis (Bacillus subtillus) is independently screened acquisition by Wuhan Kenuo Biology Science & Technology Co., Ltd., culture presevation number: CCTCC:NO.M209317, described bacillus licheniformis (Bacilluslicheniformis subsp.licheniformis) is independently screened acquisition, culture presevation number: CCTCC:NO.M208251 by Wuhan Kenuo Biology Science & Technology Co., Ltd..
Described compound probiotic, mix by a certain percentage acquisition by the former powder of bacillus subtilis and the former powder of bacillus licheniformis, wherein the former powder living spores of bacillus subtilis number is 10,000～2,000,000,000,000/g, the former powder living spores of bacillus licheniformis number is 5,000～1,000,000,000,000/g (withered grass, bacillus licheniformis preparation technology adopt respectively applicant Wuhan Kenuo Biology Science & Technology Co., Ltd. patent: ZL201010147918.0 and ZL200910063822.3), and after mixing, gemma adds up to 1.0 * 10
9～1.0 * 10
11cFU/g;
After tested, the compound symphysis of embodiment of the present invention unit formulation products also possesses following index:
Embodiment 1:
By weight percentage, select the former powder of 40% bacillus subtilis and the former powder of 60% bacillus licheniformis to mix the acquisition compound probiotic, the former powder living spores of bacillus subtilis number>=1,000,000,000,000/g wherein, the former powder living spores of bacillus licheniformis number>=500,000,000,000/g, be mixed into compound probiotic after gemma add up to 1.0 * 10
9～1.0 * 10
11cFU/g;
Selecting prebiotics is the yeast cell wall polysaccharide, wherein, by weight percentage, 35 parts of above-mentioned compound probiotics is mixed with 65 parts of prebioticses, makes the first preparation of compound symphysis.
The first preparation of above-mentioned compound symphysis is added in drinking water, add in drinking water by mass fraction 0.05%～0.2%, allow animal freely drink.Growing animal can be suitably denseer.
Embodiment 2:
By weight percentage, select the former powder of 45% bacillus subtilis (Bacillus subtillus, CCTCC NO.M209317) and the former powder of 55% bacillus licheniformis (Bacilluslicheniformis subsp.Licheniformis culture presevation number:, culture presevation number: CCTCC NO.M208251) mix the acquisition compound probiotic, the former powder living spores of bacillus subtilis number>=1,000,000,000,000/g wherein, the former powder living spores of bacillus licheniformis number>=500,000,000,000/g (withered grass and bacillus licheniformis preparation technology adopt respectively Wuhan Kenuo Biology Science & Technology Co., Ltd. from dominant patent: ZL201010147918.0 and ZL200910063822.3), after being mixed into compound probiotic, gemma adds up to 1.0 * 10
9～1.0 * 10
11cFU/g,
Selecting prebiotics is manna oligosacchride, wherein, by weight percentage, 20 parts of above-mentioned compound probiotics is mixed and makes the first preparation of compound symphysis with 65 parts of prebioticses.
The first preparation of above-mentioned compound symphysis is added in animal feed straw decomposing process: the animal feed stalk water after pulverizing or cut-out (3～5cm) is watered, permeates, the Diammonium phosphate (DAP) (with the stalk dry weight basis) of interpolation 2～5% mixes, and the stalk water content rests in 60～70%.Then in the ratio of 1: 2000, compound heat-resisting symphysis of the present invention unit is added in stalk.Play heap, obturage with the particle film, to prevent water, divide evaporation, the diffusion of heap temperature and nutrient loss.
Embodiment 3:
By weight percentage, select the former powder of 50% bacillus subtilis (Bacillus subtillus, CCTCC NO.M209317) and the former powder of 50% bacillus licheniformis (Bacilluslicheniformis subsp.Licheniformis culture presevation number:, culture presevation number: CCTCC NO.M208251) mix the acquisition compound probiotic, wherein the former powder living spores of bacillus subtilis number reaches 1,000,000,000,000/g, the former powder living spores of bacillus licheniformis number reaches 500,000,000,000/g (withered grass, bacillus licheniformis preparation technology adopts respectively Wuhan Kenuo Biology Science & Technology Co., Ltd. from dominant patent: ZL201010147918.0 and ZL200910063822.3), after being mixed into compound probiotic, gemma adds up to 1.0 * 10
9～1.0 * 10
11cFU/g,
Selecting prebiotics is the yeast cell wall polysaccharide, wherein, by weight percentage, 10 parts of above-mentioned compound probiotics is mixed with 70 parts of prebioticses, makes the first preparation of compound symphysis.
Above-mentioned compound symphysis unit preparation is added in feed: add the compound heat-resisting symphysis unit of 0.5～1.5g embodiment of the present invention in per kilogram animal basal diet, mix and want evenly, to raise every day 1 to 2 time.
By above-mentioned compound symphysis unit formulation application in the cultivation of Cherry Village Ducks, but the Partial Replacement antibiotic adds in animal feed, improve average daily gain, shorten culturing time, reduce feedstuff-meat ratio, reduce the materials amount, cost-saving, reduce the omnidistance death rate, improve the amount of delivering for sale, increase raiser's income.Concrete test data sees the following form.
Experimental animal and test method:
Select 1 age in days Cherry Village Ducks 750 plumages, be divided at random 5 groups, every group of 5 repetitions, every repetition 30 plumages.Duck is raised scattered under indoor same environment in test.Each repeats 1 hurdle, 24h illumination, and every day, feeding was 2 times, and free choice feeding and drinking-water be take and repeated to record feed injected volume and surplus every day as unit, clean water fountain and hopper every day 1 time.Immunity inoculation and prevention from suffering from the diseases, sterilization are carried out according to a conventional method.
Table 1 test material design
Test result analysis is as follows:
In test 1d (day), 14d and 35d carry out empty stomach to Cherry Village Ducks respectively and weigh, calculating counterpoise, average daily gain and material anharmonic ratio.
The impact of table 2 symphysis unit on the omnidistance growth phase production performance of Cherry Village Ducks
Finally explanation is, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although with reference to preferred embodiment, the present invention is had been described in detail, those of ordinary skill in the art is to be understood that, can modify or be equal to replacement technical scheme of the present invention, and not breaking away from aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of claim scope of the present invention.
Claims (10)
1. a compound symphysis unit preparation, comprise compound probiotic and prebiotics, it is characterized in that: described compound probiotic comprises bacillus subtilis and bacillus licheniformis, and described prebiotics is any one in yeast cell wall polysaccharide, manna oligosacchride, FOS, soyabean oligosaccharides, isomaltulose, lactosucrose, few lactose, low caramelan and few wood sugar.
2. compound symphysis according to claim 1 unit preparation is characterized in that: by weight percentage, and 10～35 parts of compound probiotics, 65～75 parts of prebioticses.
3. compound symphysis according to claim 1 unit preparation is characterized in that: the percentage by weight in described compound probiotic between two kinds of bacterium is: bacillus subtilis 30～55%, bacillus licheniformis 45～70%; Total viable count is 1.0 * 10
9～1.0 * 10
11cFU/g.
4. compound symphysis according to claim 3 unit preparation, it is characterized in that: the described bacillus subtilis of selecting (Bacillus subtillus) culture presevation number is: CCTCC NO.M209317, and the described bacillus licheniformis of selecting (Bacilluslicheniformis subsp.licheniformis) culture presevation number is: CCTCC NO.M208251; The former powder living spores of bacillus subtilis number is 10,000～2,000,000,000,000/g, and the former powder living spores of bacillus licheniformis number is 5,000～1,000,000,000,000/g.
5. compound symphysis according to claim 1 unit preparation, it is characterized in that: described prebiotics is the yeast cell wall polysaccharide.
6. compound symphysis according to claim 5 unit preparation, it is characterized in that: described yeast cell wall polysaccharide comprises glucan and mannosan, its quality percentage composition is respectively: Gentran 40～50%, and mannosan content 30～40%, other content are 20～30%.
7. according to the application of the first preparation of the described compound symphysis of claim 1～6 any one, it is characterized in that: the first feed addictive of described compound symphysis is added in animal drinking water by mass fraction 0.05%～0.2%.
8. according to the application of the first preparation of the described compound symphysis of claim 1～6 any one, it is characterized in that: the first feed addictive of described compound symphysis is added to 0.5～1.5g in per kilogram animal basal diet.
9. according to the application of the first preparation of the described compound symphysis of claim 1～6 any one, it is characterized in that: the first feed addictive of described compound symphysis is applied in animal feed straw decomposing process.
10. the application of the first preparation of compound symphysis according to claim 9, it is characterized in that: application comprises the steps: pulverizing or the animal feed stalk water after cutting off (3～5cm) waters, permeates, the Diammonium phosphate (DAP) (with the stalk dry weight basis) of interpolation 2～5% mixes, the stalk water content is 60～70%, then in the ratio of 1: 2000, described compound symphysis unit feed addictive is joined in stalk, play heap, obturage with the particle film.
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Cited By (12)
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| CN104013649A (en) * | 2014-05-29 | 2014-09-03 | 东北制药集团沈阳第一制药有限公司 | Bacillus licheniformis symbiotic composition and preparations thereof |
| CN104171421A (en) * | 2014-08-08 | 2014-12-03 | 河南牧翔动物药业有限公司 | Feed additive for conditioning intestines of animals |
| CN104381694A (en) * | 2014-11-29 | 2015-03-04 | 郑州后羿制药有限公司 | Composite microecological preparation |
| CN104605139A (en) * | 2015-01-08 | 2015-05-13 | 河北农业大学 | Bacillus subtilis-acanthopanax senticosus polysaccharide synbiotic, as well as preparation method and application thereof as feed additive |
| CN104983746A (en) * | 2015-06-18 | 2015-10-21 | 合生元（广州）健康产品有限公司 | Complex probiotics and preparation method |
| CN105053514A (en) * | 2015-07-24 | 2015-11-18 | 河南仰韶生化工程有限公司 | Compound microbial feed additive and production method thereof |
| CN105124185A (en) * | 2015-07-29 | 2015-12-09 | 江苏优仕生物科技发展有限公司 | Ingredients and preparation method of bacillus chicken feed capable of relaxing bowels |
| CN105166398A (en) * | 2015-07-29 | 2015-12-23 | 广西壮族自治区海洋研究所 | Feed enhancer special for sipunculus nudus |
| CN106578731A (en) * | 2016-12-20 | 2017-04-26 | 大连赛姆生物工程技术有限公司 | Marine synbiotics additive and application thereof in stichopus japonicus culture |
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| CN109566905A (en) * | 2018-12-29 | 2019-04-05 | 广西万寿谷投资集团股份有限公司 | A kind of Chinese herbal medicine probiotics for improving chicken immunity |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104013649A (en) * | 2014-05-29 | 2014-09-03 | 东北制药集团沈阳第一制药有限公司 | Bacillus licheniformis symbiotic composition and preparations thereof |
| CN104013649B (en) * | 2014-05-29 | 2017-05-24 | 东北制药集团沈阳第一制药有限公司 | Bacillus licheniformis symbiotic composition and preparations thereof |
| CN104171421A (en) * | 2014-08-08 | 2014-12-03 | 河南牧翔动物药业有限公司 | Feed additive for conditioning intestines of animals |
| CN104381694A (en) * | 2014-11-29 | 2015-03-04 | 郑州后羿制药有限公司 | Composite microecological preparation |
| CN104605139A (en) * | 2015-01-08 | 2015-05-13 | 河北农业大学 | Bacillus subtilis-acanthopanax senticosus polysaccharide synbiotic, as well as preparation method and application thereof as feed additive |
| CN104983746B (en) * | 2015-06-18 | 2017-05-10 | 合生元（广州）健康产品有限公司 | Complex probiotics and preparation method thereof |
| CN104983746A (en) * | 2015-06-18 | 2015-10-21 | 合生元（广州）健康产品有限公司 | Complex probiotics and preparation method |
| CN105053514A (en) * | 2015-07-24 | 2015-11-18 | 河南仰韶生化工程有限公司 | Compound microbial feed additive and production method thereof |
| CN105124185A (en) * | 2015-07-29 | 2015-12-09 | 江苏优仕生物科技发展有限公司 | Ingredients and preparation method of bacillus chicken feed capable of relaxing bowels |
| CN105166398A (en) * | 2015-07-29 | 2015-12-23 | 广西壮族自治区海洋研究所 | Feed enhancer special for sipunculus nudus |
| CN106689798A (en) * | 2016-11-17 | 2017-05-24 | 大连理工大学 | Marine synbiotic additive, preparation method thereof, as well as application of marine synbiotic additive in breeding of stichopus japonicus |
| CN106578731A (en) * | 2016-12-20 | 2017-04-26 | 大连赛姆生物工程技术有限公司 | Marine synbiotics additive and application thereof in stichopus japonicus culture |
| CN106689672A (en) * | 2017-01-04 | 2017-05-24 | 陈雨 | Synbiotics composition, synbiotics preparation, preparation method and application thereof |
| CN109566905A (en) * | 2018-12-29 | 2019-04-05 | 广西万寿谷投资集团股份有限公司 | A kind of Chinese herbal medicine probiotics for improving chicken immunity |
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