CN103461670B - Compound synbiotics preparation and its application - Google Patents
Compound synbiotics preparation and its application Download PDFInfo
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- CN103461670B CN103461670B CN201310023777.5A CN201310023777A CN103461670B CN 103461670 B CN103461670 B CN 103461670B CN 201310023777 A CN201310023777 A CN 201310023777A CN 103461670 B CN103461670 B CN 103461670B
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Abstract
The invention discloses a compound synbiotics preparation and its application. The compound synbiotics preparation includes composite Probiotics and Prebiotics, the composite Probiotics comprise Bacillus subtilis and Bacillus licheniformis, the Prebiotics are anyone of yeast cell wall polysaccharides, mannan oligosaccharides, fructo-oligosaccharides, soybean oligosaccharides, isomalto oligosaccharides, lactosucrose, galactooligosaccharides, sucrose thermal oligosaccharides caramel and oligonucleotides xylose; and the survival rate of spores is higher than 90% after the mixing granulation of the compound synbiotics preparation and a feed, and the total number of live bacteria is 1.0*10<9>-1.0*10<11>CFU/g. By utilizing the cooperative effect of different Probiotics and Prebiotics, the compound synbiotics preparation avoids the disadvantages of single Probiotics, increases the content of the Probiotics, reduces the competitor rate, improves the heat resistance and the storability of the synbiotics, improves the quality of feeding synbiotics, and has a wide application prospect.
Description
Technical field
The present invention relates to microorganism fodder additive field, especially relate to a kind of compound symphysis unit preparation.
Background technology
Along with the development of aviculture, each state all takes many kinds of measures to control to purify poultry disease.But the problems such as the drug residue of food that antibiotic extensive Long-Time Service causes and microorganism drug resistance, have carried out huge risk to cultivation industrial belt.
Thus, people start to propose strict requirement to the chemical residual of meat, egg food, and the application of chemical prevention agent in animal-breeding is restricted, and Europe prohibits especially and use any antibiotic in animal diets.Adopt which kind of measure to replace antibiotic application, above-mentioned risk can not be brought simultaneously to ensure again the health of animal, become the Tough questions urgently to be resolved hurrily that aquaculture faces.
For this reason, the theory that animal microecological educational circles proposes " the final using microbe of problem of microorganism solves ", under the guide of this theory, the technology of biological competition method control disease causes the concern of scholar, animal microecology (Animal Microecology) obtains and develops (Tomohiro H, 1983 rapidly; Xu C L, 2006).Utilize the principle of " fungus treatment " in recent years, use probio (Probiotics) to prevent disease from infecting from microecology angle, obtain the extensive accreditation of international academic community, become one of focus of current application microbial technique research.
Calendar year 2001, the World Food Programme and the World Health Organization have done as given a definition to probio, " by absorbing suitable amount, the healthy viable bacteria that can play useful effect to eater ".Feeding probio produces bacterial strain and not only requires that prebiotic function is remarkable, also must can tolerate the high temperature in feed manufacturing process, and can low cost, large scale fermentation cultivation.At present, domestic and international application the most widely probio is lactic acid bacteria class, and the prebiotic function of lactic acid bacteria class is remarkable, but can not tolerate the high temperature in feed manufacturing process, unstable product quality because of it, and application potential and scope are restricted.
Prebiotics (Prebiotics), referring to can the breeding of the profitable strain of field planting or a kind of dietary supplements of activity in selective stimulating enteron aisle, it can not utilize for livestock and poultry own absorption, can not be again that in enteron aisle, most of harmful bacteria utilizes, and can only be that most of probio utilizes, thus make beneficial bacterium hyperplasia.
Along with deepening continuously of research, people transfer use composite bacteria to from use single culture gradually, transfer application viable bacteria to from only using active bacteria formulation and have regulates the non-viable bacteria prebiotic component of animal microecological environment the to carry out first preparation of symphysis that compound synergic makes.
Symphysis unit (synbiotics) is also called Synbiotics, refers to the combination preparation of probio and prebiotics, or adds vitamin, trace element etc. again.It both can play the physiological bacterial activity of probio, optionally increased again the quantity of this bacterium fast, made probiotic action significantly lasting.Symphysis unit, as microecologic regulator of new generation, by probio and prebiotics use in conjunction, can play the physiological function of probio and prebiotics simultaneously, make probio and prebiotics coordinative role, jointly to anti-disease, safeguard the microecological balance of body.Compare simple probio or prebiotics, symphysis unit has better cost performance, and market potential is larger.But, because current animal feed generally adopts steam regulation of texture, conditioning of texture granulation technique, a large amount of heat labile microorganism can kill by the hot and humid condition of this process, therefore symphysis unit is as feed addictive, and wherein the resistance to elevated temperatures of viable bacteria is one of bottleneck of restriction probiotics application, in addition, it is low also to there is bacteria containing amount in the feeding symphysis unit of current production, the shortcomings such as miscellaneous bacteria rate is high, and quality is unstable, seriously constrain development and the application of China's feeding micro-ecological preparation.
Summary of the invention
Main purpose of the present invention is to provide a kind of compound symphysis unit preparation, this compound symphysis unit preparation utilizes the cooperative effect of different probio and prebiotics, not only avoid the deficiency of single probio, but also add probio content, reduce miscellaneous bacteria rate, and improve heat resistance and the storage property of described symphysis unit, improve feeding symphysis unit quality, have broad application prospects.
In order to reach object of the present invention, the present invention is by the following technical solutions: a kind of compound symphysis unit preparation, comprise compound probiotic and prebiotics, described compound probiotic comprises bacillus subtilis and bacillus licheniformis, and described prebiotics is any one in yeast cell wall polysaccharide, manna oligosacchride, lactosucrose, few lactose, low caramelan and few wood sugar; Described bacillus subtilis (Bacillus subtillus) culture presevation number of selecting is: CCTCC NO.M209317, described in bacillus licheniformis (Bacilluslicheniformis subsp.licheniformis) culture presevation number of selecting be: CCTCC NO.M208251; Bacillus subtilis former powder living spores number is 10,000 ~ 2,000,000,000,000/g, bacillus licheniformis former powder living spores number 5,000 ~ 1,000,000,000,000/g.
Preferably, described compound symphysis unit preparation, by weight percentage, compound probiotic 10 ~ 35 parts, prebiotics 65 ~ 75 parts.
Preferably, the percentage by weight in described compound probiotic between two kinds of bacterium is: bacillus subtilis 30 ~ 55%, bacillus licheniformis 45 ~ 70%; After mixing, total viable count is 1.0 × 10
9~ 1.0 × 10
11cFU/g.
Preferably, described prebiotics is yeast cell wall polysaccharide.
Preferably, described yeast cell wall polysaccharide comprises glucan, mannosan, and its mass percentage is respectively: Gentran 40 ~ 50%, mannan content 30 ~ 40%, and other content are 20 ~ 30%.
The invention has the beneficial effects as follows: the present invention's compound symphysis unit preparation has high-purity, high activity, low miscellaneous bacteria and the advantage such as high temperature resistant.This kind of symphysis unit mixes from different animal feeds, pernicious bacteria survival ability in animal body can be suppressed, strengthen animal immunizing power, reduce the use amount of the chemicals such as veterinary antibiotic, hormone in aquaculture, improve meat product safety, and improve the meat of animal, increase the delicate flavour of muscle.
Detailed description of the invention
Below by embodiment, the present invention is further detailed.
The embodiment of the present invention compound symphysis unit preparation, comprise compound probiotic and prebiotics, described compound probiotic comprises bacillus subtilis and bacillus licheniformis, and described prebiotics is any one in yeast cell wall polysaccharide, manna oligosacchride, FOS, soyabean oligosaccharides, isomaltulose, lactosucrose, few lactose, low caramelan and few wood sugar; Symphysis unit preparation and feed mixing granulation after gemma survival rate higher than 90%, total viable count is 1.0 × 10
9~ 1.0 × 10
11cFU/g.
Described compound symphysis unit preparation, by weight percentage, compound probiotic 10 ~ 35 parts, prebiotics 65 ~ 75 parts.
Percentage by weight in described compound probiotic between two kinds of bacterium is: bacillus subtilis 30 ~ 55%, bacillus licheniformis 45 ~ 70%.
Described bacillus subtilis (Bacillus subtillus) independently screens acquisition by Wuhan Kenuo Biology Science & Technology Co., Ltd., culture presevation number: CCTCC:NO.M209317, described bacillus licheniformis (Bacilluslicheniformis subsp.licheniformis) independently screens acquisition by Wuhan Kenuo Biology Science & Technology Co., Ltd., culture presevation number: CCTCC:NO.M208251.
Described compound probiotic, acquisition is mixed by a certain percentage by the former powder of bacillus subtilis and the former powder of bacillus licheniformis, wherein bacillus subtilis former powder living spores number is 10,000 ~ 2,000,000,000,000/g, bacillus licheniformis former powder living spores number is that (withered grass, bacillus licheniformis preparation technology adopt applicant Wuhan Kenuo Biology Science & Technology Co., Ltd. patent to 5,000 ~ 1,000,000,000,000/g respectively: ZL201010147918.0 and ZL200910063822.3), and after mixing, total spore content is 1.0 × 10
9~ 1.0 × 10
11cFU/g;
After tested, the embodiment of the present invention compound symphysis unit formulation products also possesses following index:
Embodiment 1:
By weight percentage, the former powder of 40% bacillus subtilis and the former powder of 60% bacillus licheniformis is selected to mix acquisition compound probiotic, wherein bacillus subtilis former powder living spores number>=1,000,000,000,000/g, bacillus licheniformis former powder living spores number>=500,000,000,000/g, after being mixed into compound probiotic, total spore content is 1.0 × 10
9~ 1.0 × 10
11cFU/g;
Select prebiotics to be yeast cell wall polysaccharide, wherein, by weight percentage, above-mentioned compound probiotic 35 parts and prebiotics 65 parts are mixed, obtained compound symphysis unit preparation.
Above-mentioned compound symphysis unit preparation is added in drinking water, adds in drinking water by mass fraction 0.05% ~ 0.2%, allow animal freely drink.Growing animal can be suitably denseer.
Embodiment 2:
By weight percentage, select the former powder of 45% bacillus subtilis (Bacillus subtillus, culture presevation number: CCTCC NO.M209317) and the former powder of 55% bacillus licheniformis (Bacilluslicheniformis subsp.Licheniformis, culture presevation number: CCTCC NO.M208251) mix acquisition compound probiotic, wherein bacillus subtilis former powder living spores number>=1,000,000,000,000/g, (withered grass and bacillus licheniformis preparation technology adopt Wuhan Kenuo Biology Science & Technology Co., Ltd. from dominant patent to bacillus licheniformis former powder living spores number>=500,000,000,000/g respectively: ZL201010147918.0 and ZL200910063822.3), after being mixed into compound probiotic, total spore content is 1.0 × 10
9~ 1.0 × 10
11cFU/g,
Select prebiotics to be manna oligosacchride, wherein, by weight percentage, above-mentioned compound probiotic 20 parts and prebiotics 65 parts are mixed obtained compound symphysis unit preparation.
Above-mentioned compound symphysis unit preparation is added in animal feed straw decomposing process: pulverizing or the animal feed stalk water after cutting off (3 ~ 5cm) are watered, permeated, the Diammonium phosphate (DAP) (with stalk dry weight basis) of adding 2 ~ 5% mixes, and stalk water content rests in 60 ~ 70%.Then in the ratio of 1: 2000, compound heat-resisting symphysis unit of the present invention is added in stalk.Play heap, obturage with particle film, divide evaporation, the diffusion of heap temperature and nutrient loss to prevent water.
Embodiment 3:
By weight percentage, select the former powder of 50% bacillus subtilis (Bacillus subtillus, culture presevation number: CCTCC NO.M209317) and the former powder of 50% bacillus licheniformis (Bacilluslicheniformis subsp.Licheniformis, culture presevation number: CCTCC NO.M208251) mix acquisition compound probiotic, wherein bacillus subtilis former powder living spores number reaches 1,000,000,000,000/g, bacillus licheniformis former powder living spores number reaches 500,000,000,000/g (withered grass, bacillus licheniformis preparation technology adopts Wuhan Kenuo Biology Science & Technology Co., Ltd. from dominant patent respectively: ZL201010147918.0 and ZL200910063822.3), after being mixed into compound probiotic, total spore content is 1.0 × 10
9~ 1.0 × 10
11cFU/g,
Select prebiotics to be yeast cell wall polysaccharide, wherein, by weight percentage, above-mentioned compound probiotic 10 parts and prebiotics 70 parts are mixed, obtained compound symphysis unit preparation.
Above-mentioned compound symphysis unit preparation is added in feed: the compound heat-resisting symphysis unit adding 0.5 ~ 1.5g embodiment of the present invention in per kilogram animal basal diet, mixing is wanted evenly, and every day raises 1 to 2 time.
By above-mentioned compound symphysis unit formulation application in the cultivation of Cherry Village Ducks, antibiotic can be partly replaced to add in animal feed, improve average daily gain, shorten culturing time, reduce feedstuff-meat ratio, minimizing doses, cost-saving, reduce the omnidistance death rate, improve the amount of delivering for sale, increase raiser's income.Concrete test data sees the following form.
Experimental animal and test method:
Select 1 age in days Cherry Village Ducks 750 plumage, be divided into 5 groups at random, often organize 5 repetitions, often repeat 30 plumages.Duck is raised scattered under the same environment in indoor in test.Each repetition 1 hurdle, 24h illumination, every day feeding 2 times, free choice feeding and drinking-water, in units of repeating, every day records feed injected volume and surplus, cleans water fountain and hopper 1 time every day.Immunity inoculation and prevention from suffering from the diseases, sterilization are carried out according to a conventional method.
Table 1 test material design
Test result analysis is as follows:
In test 1d (my god), 14d and 35d carries out empty stomach to Cherry Village Ducks respectively and weighs, and calculates counterpoise, average daily gain and feed-weight ratio.
Show 2-in-1 raw unit to the impact of the omnidistance growth phase production performance of Cherry Village Ducks
What finally illustrate is, above embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although with reference to preferred embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that, can modify to technical scheme of the present invention or equivalent replacement, and not departing from aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of right of the present invention.
Claims (1)
1. a compound symphysis unit preparation, it is made up of compound probiotic and prebiotics, it is characterized in that: by weight percentage, compound probiotic 10 ~ 35 parts, prebiotics 65 ~ 75 parts, described compound probiotic is bacillus subtilis and bacillus licheniformis, percentage by weight in described compound probiotic between two kinds of bacterium is: bacillus subtilis 30 ~ 55%, bacillus licheniformis 45 ~ 70%; Total viable count is 1.0 × 10
9~ 1.0 × 10
11cFU/g; Described prebiotics is yeast cell wall polysaccharide, and described yeast cell wall polysaccharide comprises glucan and mannosan, and its mass percentage is respectively: Gentran 40 ~ 50%, mannan content 30 ~ 40%, and other content are 20 ~ 30%; Described bacillus subtilis (Bacillus subtillus) culture presevation number of selecting is: CCTCC NO.M209317, described in bacillus licheniformis (Bacilluslicheniformis subsp.licheniformis) culture presevation number of selecting be: CCTCC NO.M208251; Bacillus subtilis former powder living spores number is 10,000 ~ 2,000,000,000,000/g, and bacillus licheniformis former powder living spores number is 5,000 ~ 1,000,000,000,000/g.
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| CN104013649B (en) * | 2014-05-29 | 2017-05-24 | 东北制药集团沈阳第一制药有限公司 | Bacillus licheniformis symbiotic composition and preparations thereof |
| CN104171421A (en) * | 2014-08-08 | 2014-12-03 | 河南牧翔动物药业有限公司 | Feed additive for conditioning intestines of animals |
| CN104381694A (en) * | 2014-11-29 | 2015-03-04 | 郑州后羿制药有限公司 | Composite microecological preparation |
| CN104605139A (en) * | 2015-01-08 | 2015-05-13 | 河北农业大学 | Bacillus subtilis-acanthopanax senticosus polysaccharide synbiotic, as well as preparation method and application thereof as feed additive |
| CN104983746B (en) * | 2015-06-18 | 2017-05-10 | 合生元（广州）健康产品有限公司 | Complex probiotics and preparation method thereof |
| CN105053514A (en) * | 2015-07-24 | 2015-11-18 | 河南仰韶生化工程有限公司 | Compound microbial feed additive and production method thereof |
| CN105166398A (en) * | 2015-07-29 | 2015-12-23 | 广西壮族自治区海洋研究所 | Feed enhancer special for sipunculus nudus |
| CN105124185A (en) * | 2015-07-29 | 2015-12-09 | 江苏优仕生物科技发展有限公司 | Ingredients and preparation method of bacillus chicken feed capable of relaxing bowels |
| CN106689798B (en) * | 2016-11-17 | 2021-03-30 | 大连理工大学 | Marine synbiotic additive, preparation method thereof and application thereof in stichopus japonicus culture |
| CN106578731A (en) * | 2016-12-20 | 2017-04-26 | 大连赛姆生物工程技术有限公司 | Marine synbiotics additive and application thereof in stichopus japonicus culture |
| CN106689672A (en) * | 2017-01-04 | 2017-05-24 | 陈雨 | Synbiotics composition, synbiotics preparation, preparation method and application thereof |
| CN109566905A (en) * | 2018-12-29 | 2019-04-05 | 广西万寿谷投资集团股份有限公司 | A kind of Chinese herbal medicine probiotics for improving chicken immunity |
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