CN104983746B - Complex probiotics and preparation method thereof - Google Patents
Complex probiotics and preparation method thereof Download PDFInfo
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- CN104983746B CN104983746B CN201510344591.9A CN201510344591A CN104983746B CN 104983746 B CN104983746 B CN 104983746B CN 201510344591 A CN201510344591 A CN 201510344591A CN 104983746 B CN104983746 B CN 104983746B
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Abstract
The invention discloses complex probiotics and a preparation method. The complex probiotics comprise, by weight, 2-25 parts of probiotics, 50-200 parts of prebiotics, 300-500 parts of dietary fiber, 50-100 parts of amino acid and 10-50 parts of vitamin B. The complex probiotics can effectively adjust the intestinal florae, promote proliferation growth of probiotics in the intestinal tract, guarantee the living probiotic amount of the probiotics in the intestinal tract and enhance the healthcare function. According to the complex probiotics, the probiotics are combined with the prebiotics, the dietary fiber and the like and promote one another, and a good synergistic effect is achieved.
Description
Technical field
The present invention relates to a kind of probiotic composition, and in particular to a kind of compound probiotic and preparation method thereof.
Background technology
Probio (Probiotics), earliest from Greek, means " beneficial to life ", and referring to can improve host
The balance of (such as animals or humans) enteral Tiny ecosystem, and have the active microorganism of front benefit to host, with alleviation intolerant to breast
Sugared symptom, prevention genital system infection, enhancing body immunity, reduce serum cholesterol, help absorb nutrient content, strengthen and plant
Thing metabolism etc. is acted on.In human body, animal body beneficial bacterium or fungi mainly have clostridium butyricum, lactic acid bacteria, Bifidobacterium,
Lactobacillus acidophilus, actinomyces, saccharomycete etc., it is widely used in bioengineering, industrial or agricultural, food security and life and health
Field.
Probio is the microorganism for having vitality, from production (including bacteria selection, production environment, packaging etc.),
Shelf are for sale, it is edible after reach that enteron aisle -- this tests layer by layer the work that can consume probio through extreme environments such as hydrochloric acid in gastric juice, bile
Property, the probio for only through these outposts of the tax office and pinpointing reproduction survival in enteron aisle eventually could really play a role.Yet with Jing
Long-term loss is crossed, the viable bacteria number of probiotic products of the existing probiotic products generally in human body is reached is reduced, it is difficult to
Guarantee eventually arrives at the viable bacteria amount of large intestine.
The content of the invention
To overcome the defect of prior art, it is an object of the invention to provide a kind of compound probiotic, is a kind of probio
Composition, improves the activity of probio, strengthens its function.
Another object of the present invention is to provide a kind of preparation method of compound probiotic.
For achieving the above object the technical solution adopted in the present invention is as follows：
A kind of compound probiotic, the compound probiotic includes probio, prebiotics, dietary fiber, amino acid and dimension life
Plain B, the content of each component is as follows respectively in the compound probiotic：
Further, the content of each component is as follows respectively in the compound probiotic：
Specifically, heretofore described probio is bifidobacterium lactis, Lactobacillus rhamnosus, lactobacillus acidophilus, animal pair
One or more mixing in discrimination bacillus；Wherein, probiotics viable bacteria amount is 107～1010CFU/g。
Specifically, heretofore described prebiotics is in fructooligosaccharide, isomalto-oligosaccharide, galactooligosaccharide, inulin
One or two mixing；The fructooligosaccharide includes ketose, Nystose and GF4；The different malt is oligomeric
Sugar includes isomaltose, panose and Isomaltotriose.
Specifically, heretofore described dietary fiber be pentosan, β -1,3 glucans, β-Isosorbide-5-Nitrae glucan, solubility
One or more mixing in pectin, araban, SKGM, guar gum.
Specifically, heretofore described amino acid is alanine, valine, leucine, isoleucine, proline, phenylpropyl alcohol
Propylhomoserin, tryptophan, methionine glycine, serine, threonine, cysteine, tyrosine, asparagine, glutamine, bad ammonia
One or more mixing in acid, arginine, histidine, aspartic acid, glutamic acid.
Specifically, heretofore described Cobastab is the one kind in vitamin B1, vitamin B12.
The step of method for preparing compound probiotic of the present invention, the method, is as follows：
1) 4-5 times of weight is placed in the dietary fiber of probio and is put in the mixer of emptying with medical nitrogen, Ran Houjia
Enter probio, be well mixed；
2) treat step 1) after the completion of add prebiotics, amino acid and the Cobastab of remaining dietary fiber and formula ratio,
It is well mixed, aseptic lower packaging.
Compared to existing technology, the beneficial effects of the present invention is：
1. compound probiotic of the present invention can effective regulating intestinal canal flora, promote probio to rise in value in enteron aisle life
It is long, it is ensured that viable bacteria amount of the probio in enteron aisle, strengthen health care；
2. in the present invention, probio and prebiotics, dietary fiber etc. combine, and mutually promote, and make with good collaboration
With；
3. compound probiotic of the present invention can process the different agent such as piece agent, pulvis, powder, particle, pill
Type, it is also possible to be added in other products (dairy products etc.) as additive, be a kind of health products of safety and Health.
Specific embodiment
The present invention is described in further detail with reference to specific embodiment.
The present invention provides a kind of compound probiotic, and the compound probiotic includes probio, prebiotics, dietary fiber, amino
Acid and Cobastab, the content of each component is as follows respectively in the compound probiotic：
Further, the content of each component is as follows respectively in the compound probiotic：
Specifically, heretofore described probio is bifidobacterium lactis, Lactobacillus rhamnosus, lactobacillus acidophilus, animal pair
One or more mixing in discrimination bacillus；Wherein, probiotics viable bacteria amount is 107～1010CFU/g.In the present invention, specifically
The bifidobacterium lactis for adopting are HN019 bacterial strains or Bi-07 bacterial strains for bifidobacterium lactis, Lactobacillus rhamnosus be HN001 bacterial strains or
LGG bacterial strains, lactobacillus acidophilus is NCFM bacterial strains, and animal bifidobacteria is Bb-12 bacterial strains.Wherein animal bifidobacteria Bb-12 and
Lactobacillus rhamnosus LGG is produced by Hansen Corp. of section of Denmark, and other 4 plants of bacterium-lactobacillus acidophilus NCFM, bifidobacterium lactis
HN019, bifidobacterium lactis Bi-07, Lactobacillus rhamnosus HN001 are produced by Danisco A/S BJ Rep Office of Du Pont.Probiotic strain is in stomach and intestine
Can produce digestive ferment in road, these enzymes human body can be helped preferably to digest taken in food and absorb nutrition in food into
Point.Probio also contestable suppresses harmful microorganism to absorb nutriment and enter blood circulation system；Lactobacillus acidophilus can
The lactase of secretion digestion lactose, so as to alleviate lactose intolerance.
Specifically, heretofore described prebiotics is in fructooligosaccharide, isomalto-oligosaccharide, galactooligosaccharide, inulin
One or two mixing；The fructooligosaccharide includes ketose, Nystose and GF4；The different malt is oligomeric
Sugar includes isomaltose, panose and Isomaltotriose；Wherein it is preferably fructooligosaccharide, isomalto-oligosaccharide.In the present invention
The prebiotics of middle addition can decompose absorption by beneficial bacteria in enteron aisle, promote beneficial bacteria growth and breeding, it is ensured that probio reaches
To the viable bacteria amount of enteron aisle.
Heretofore described dietary fiber is water miscible dietary fiber, can specifically be adopted as pentosan, β -1, and 3 Portugals gather
One or more mixing in sugar, beta-1,4 glucose, soluble pectin, araban, SKGM, guar gum.
Dietary fiber has very strong water absorbing capacity or the ability combined with water, and this effect increases can the volume of excrement in enteron aisle, accelerate
Its transport speed, reduces the time that wherein harmful substance contacts intestinal wall；Dietary fiber is in enteron aisle easily by bacterium glycolysis, and solubility is fine
Dimension so as to the growth that can promote probio in the present invention, can keep the activity of probio completely by bacterium glycolysis.
Specifically, heretofore described amino acid is alanine, valine, leucine, isoleucine, proline, phenylpropyl alcohol
Propylhomoserin, tryptophan, methionine glycine, serine, threonine, cysteine, tyrosine, asparagine, glutamine, bad ammonia
One or more mixing in acid, arginine, histidine, aspartic acid, glutamic acid.
Specifically, heretofore described Cobastab is one or two mixing in vitamin B1, vitamin B12.Due to
There is synergy between the vitamin of B races, therefore in preferred embodiments of the present invention, mixed using vitamin B1 and vitamin B12
Close.
The step of method for preparing compound probiotic of the present invention, the method, is as follows：
1) 4-5 times of weight is placed in the dietary fiber of probio and is put in the mixer of emptying with medical nitrogen, Ran Houjia
Enter probio, be well mixed；
2) treat step 1) after the completion of add prebiotics, amino acid and the Cobastab of remaining dietary fiber and formula ratio,
It is well mixed, aseptic lower packaging.
Embodiment 1
A kind of compound probiotic, the compound probiotic includes probio, prebiotics, dietary fiber, amino acid and dimension life
Plain B, the content of each component is as follows respectively in the compound probiotic：
It is prepared by the following method and forms：
1) by 4 times of weight in β -1 of bifidobacterium lactis, 3 glucans are placed in be put in the mixer of emptying with medical nitrogen,
Probio is subsequently adding, is well mixed；
2) treat step 1) after the completion of add the fructooligosaccharide of remaining β -1,3 glucans and formula ratio, aspartic acid with
And vitamin B1, it is well mixed, aseptic lower packaging.
Embodiment 2
A kind of compound probiotic, the compound probiotic includes probio, prebiotics, dietary fiber, amino acid and dimension life
Plain B, the content of each component is as follows respectively in the compound probiotic：
It is prepared by the following method and forms：
1) 4 times of weight are placed in β -1,3 glucans of bifidobacterium lactis+Lactobacillus rhamnosus and put row with medical nitrogen
In empty mixer, probio is subsequently adding, is well mixed；
2) treat step 1) after the completion of add isomalto-oligosaccharide, the aspartic acid of remaining β -1,3 glucans and formula ratio
And vitamin B12, it is well mixed, aseptic lower packaging.
Embodiment 3
A kind of compound probiotic, the compound probiotic includes probio, prebiotics, dietary fiber, amino acid and dimension life
Plain B, the content of each component is as follows respectively in the compound probiotic：
It is prepared by the following method and forms：
1) 5 times of weight are placed in the mixer that emptying is put with medical nitrogen in the beta-1,4 glucose of Lactobacillus rhamnosus
It is interior, probio is subsequently adding, it is well mixed；
2) treat step 1) after the completion of add the galactooligosaccharide of remaining beta-1,4 glucose and formula ratio, aspartic acid with
And vitamin B12, it is well mixed, aseptic lower packaging.
Embodiment 4
A kind of compound probiotic, the compound probiotic includes probio, prebiotics, dietary fiber, amino acid and dimension life
Plain B, the content of each component is as follows respectively in the compound probiotic：
It is prepared by the following method and forms：
1) 5 times of weight are placed in the beta-1,4 glucose of bifidobacterium lactis+lactobacillus acidophilus and put emptying with medical nitrogen
Mixer in, be subsequently adding probio, be well mixed；
2) treat step 1) after the completion of add galactooligosaccharide+inulin, the asparagus fern of remaining beta-1,4 glucose and formula ratio
Propylhomoserin and vitamin B1+vitamin B12, are well mixed, aseptic lower packaging.
Function test
Compound probiotic regulating intestinal canal flora function test to the present invention
Experimenter：25-45 year, totally 50, wherein men and women is each 25, wherein 25 used as experimental group, another 25 people is used as right
According to group, exclude gestation and women breast-feeding their children, exclude allergic constitution and to health food allergy sufferers, exclusion have angiocarpy, liver, kidney and
The serious disease persons such as hemopoietic system, subjects signed voluntarily signs book of knowing the inside story.
Dosage and usage：Using the product of embodiment 3, granule is processed into, is a secondary amounts per 2g, experimental group personnel are daily
Taken once with 35-40 DEG C of warm water, continuous 14 days.Control group is left intact factor.During testing, experimental group and control
Group is according to daily eating habit and work and rest custom.
Indexs measure：1. the spirit, sleep, blood pressure and stool and urine situation during testing experimental group and control group is seen
Examine；2. entrust Grade A hospital that routine blood test, just routine urinalysis, conventional, blood biochemistry index and electrocardiogram, ultrasound diagnosis are carried out to experimenter；
3. its functional attributes：The censorship immediately of experimenter's fresh excreta, is detected according to the method in GB GB4789.2-1994.
1. number of live bacteria of probiotics the results are shown in Table 1.
Table 1
2. experimenter's safety indexes are shown in Table 2.
Table 2
Above-mentioned embodiment is only the preferred embodiment of the present invention, it is impossible to limit the scope of protection of the invention with this,
The change and replacement of any unsubstantiality that those skilled in the art is done on the basis of the present invention belongs to institute of the present invention
Claimed scope.
Claims (1)
1. a kind of compound probiotic composition, it is characterised in that the content of each component is respectively in the compound probiotic：
Weight portion 1:1 bifidobacterium lactis and lactobacillus acidophilus 25g, weight portion 1:1 galactooligosaccharide and inulin 150g, β -1,
4 Gentran 40 0g, aspartic acid 50g and vitamin B12 30g,
Its preparation method is：
1) 5 times of weight are placed in the beta-1,4 glucose of bifidobacterium lactis and lactobacillus acidophilus and put the mixed of emptying with medical nitrogen
In conjunction machine, bifidobacterium lactis and lactobacillus acidophilus are subsequently adding, are well mixed；
2) step 1 is treated) after the completion of, add galactooligosaccharide and inulin, the aspartic acid of remaining β-Isosorbide-5-Nitrae glucan and formula ratio
And vitamin B12, it is well mixed, aseptic lower packaging.
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| CN106890317A (en) * | 2015-12-17 | 2017-06-27 | 吴波峰 | A kind of lactase composition and its application |
| CN106721843B (en) * | 2016-11-30 | 2020-05-08 | 汉臣氏（沈阳）儿童制品有限公司 | Double-bacterial protein solid beverage |
| CN106617092A (en) * | 2016-12-13 | 2017-05-10 | 合肥赛为智慧医疗有限公司 | Cancer prevention and resistance probiotics preparation and preparation method thereof |
| CN107594284A (en) * | 2017-10-23 | 2018-01-19 | 南京邦康生物技术有限公司 | A kind of solid beverage containing prebiotics and probiotics and preparation method thereof |
| CN107821896A (en) * | 2017-12-05 | 2018-03-23 | 合肥海昭生物科技有限公司 | A kind of live body compound probiotic drink |
| CN108813262A (en) * | 2018-07-02 | 2018-11-16 | 杭州相生相成科技有限公司 | A kind of compound probiotic solid beverage containing DHA algal oil |
| CN108741091A (en) * | 2018-08-29 | 2018-11-06 | 安徽谷益生物科技有限公司 | A kind of compound probiotic functional food for adjusting infantile enteritis immune response |
| CN109430407A (en) * | 2018-09-10 | 2019-03-08 | 迪拜尔特控股（北京）有限公司 | Probiotic combinations preparation |
| CN109287920A (en) * | 2018-09-14 | 2019-02-01 | 安吉艾格赛思生物科技有限公司 | A kind of solid beverage and preparation method thereof of multicomponent complex and multi-joint probiotics |
| CN112189850A (en) * | 2020-09-17 | 2021-01-08 | 合生元（广州）健康产品有限公司 | Use of prebiotics to promote survival of probiotic bacteria in the gastrointestinal tract |
| CN112546160A (en) * | 2020-12-23 | 2021-03-26 | 拉格比(广东)健康科技有限公司 | Probiotic preparation for improving diabetes and preparation method thereof |
| CN113287751A (en) * | 2021-05-07 | 2021-08-24 | 济南万泉生物技术有限公司 | Composite probiotics and preparation method and application thereof |
| CN116349815A (en) * | 2021-12-28 | 2023-06-30 | 自然阳光(上海)日用品有限公司 | Production formula of viable bacteria type probiotic solid beverage capable of regulating intestinal flora |
| CN117413936B (en) * | 2023-11-24 | 2024-05-24 | 广东汉基天承基因科技有限公司 | Active liquid for regulating gastrointestinal functions and preparation method thereof |
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