CN105802876A - Composite probiotic-fermented alfalfa sprout powder preparation and preparation method and application thereof - Google Patents
Composite probiotic-fermented alfalfa sprout powder preparation and preparation method and application thereof Download PDFInfo
- Publication number
- CN105802876A CN105802876A CN201610171344.8A CN201610171344A CN105802876A CN 105802876 A CN105802876 A CN 105802876A CN 201610171344 A CN201610171344 A CN 201610171344A CN 105802876 A CN105802876 A CN 105802876A
- Authority
- CN
- China
- Prior art keywords
- herba medicaginis
- tender shoots
- powder
- powder preparation
- cgmcc
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000843 powder Substances 0.000 title claims abstract description 98
- 238000002360 preparation method Methods 0.000 title claims abstract description 47
- 239000002131 composite material Substances 0.000 title claims abstract description 30
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 title abstract description 27
- 241000219823 Medicago Species 0.000 title abstract 5
- 239000006041 probiotic Substances 0.000 claims abstract description 99
- 235000018291 probiotics Nutrition 0.000 claims abstract description 99
- 241000894006 Bacteria Species 0.000 claims abstract description 63
- 238000000855 fermentation Methods 0.000 claims abstract description 63
- 230000004151 fermentation Effects 0.000 claims abstract description 63
- 230000000529 probiotic effect Effects 0.000 claims abstract description 55
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 46
- 244000199866 Lactobacillus casei Species 0.000 claims abstract description 25
- 108010073771 Soybean Proteins Proteins 0.000 claims abstract description 18
- 235000019710 soybean protein Nutrition 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 229930006000 Sucrose Natural products 0.000 claims abstract description 16
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 16
- 239000005720 sucrose Substances 0.000 claims abstract description 15
- 239000003826 tablet Substances 0.000 claims abstract description 14
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 13
- 239000002775 capsule Substances 0.000 claims abstract description 13
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 13
- 235000013958 Lactobacillus casei Nutrition 0.000 claims abstract description 10
- 229940017800 lactobacillus casei Drugs 0.000 claims abstract description 10
- 241001134770 Bifidobacterium animalis Species 0.000 claims abstract description 8
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims abstract description 8
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000000811 xylitol Substances 0.000 claims abstract description 8
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims abstract description 8
- 229960002675 xylitol Drugs 0.000 claims abstract description 8
- 235000010447 xylitol Nutrition 0.000 claims abstract description 8
- 229940118852 bifidobacterium animalis Drugs 0.000 claims abstract description 6
- 239000001963 growth medium Substances 0.000 claims abstract description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 12
- 239000002054 inoculum Substances 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 11
- 241000186660 Lactobacillus Species 0.000 claims description 10
- 229940039696 lactobacillus Drugs 0.000 claims description 10
- 238000004108 freeze drying Methods 0.000 claims description 8
- 239000002609 medium Substances 0.000 claims description 8
- 239000003223 protective agent Substances 0.000 claims description 8
- 239000012153 distilled water Substances 0.000 claims description 6
- 239000002068 microbial inoculum Substances 0.000 claims description 6
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 5
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 5
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 5
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 5
- 239000008101 lactose Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 4
- 241000196324 Embryophyta Species 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 2
- 238000004321 preservation Methods 0.000 abstract description 3
- 238000011081 inoculation Methods 0.000 abstract 1
- 238000009777 vacuum freeze-drying Methods 0.000 abstract 1
- 240000004658 Medicago sativa Species 0.000 description 23
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 18
- ZQSIJRDFPHDXIC-UHFFFAOYSA-N daidzein Chemical compound C1=CC(O)=CC=C1C1=COC2=CC(O)=CC=C2C1=O ZQSIJRDFPHDXIC-UHFFFAOYSA-N 0.000 description 16
- 241000901050 Bifidobacterium animalis subsp. lactis Species 0.000 description 14
- 230000000694 effects Effects 0.000 description 13
- 229960004793 sucrose Drugs 0.000 description 13
- 235000012054 meals Nutrition 0.000 description 12
- 238000000034 method Methods 0.000 description 11
- GOMNOOKGLZYEJT-UHFFFAOYSA-N isoflavone Chemical compound C=1OC2=CC=CC=C2C(=O)C=1C1=CC=CC=C1 GOMNOOKGLZYEJT-UHFFFAOYSA-N 0.000 description 10
- CJWQYWQDLBZGPD-UHFFFAOYSA-N isoflavone Natural products C1=C(OC)C(OC)=CC(OC)=C1C1=COC2=C(C=CC(C)(C)O3)C3=C(OC)C=C2C1=O CJWQYWQDLBZGPD-UHFFFAOYSA-N 0.000 description 10
- 235000008696 isoflavones Nutrition 0.000 description 10
- 239000004310 lactic acid Substances 0.000 description 9
- 235000014655 lactic acid Nutrition 0.000 description 9
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 8
- LXNHXLLTXMVWPM-UHFFFAOYSA-N Vitamin B6 Natural products CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 8
- 235000007240 daidzein Nutrition 0.000 description 8
- 235000013325 dietary fiber Nutrition 0.000 description 8
- 230000001954 sterilising effect Effects 0.000 description 8
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 7
- 210000004369 blood Anatomy 0.000 description 7
- 239000008280 blood Substances 0.000 description 7
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 7
- 150000001875 compounds Chemical class 0.000 description 7
- 229930003944 flavone Natural products 0.000 description 7
- 235000011949 flavones Nutrition 0.000 description 7
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- 239000011726 vitamin B6 Substances 0.000 description 6
- ZCOLJUOHXJRHDI-FZHKGVQDSA-N Genistein 7-O-glucoside Natural products O([C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O1)c1cc(O)c2C(=O)C(c3ccc(O)cc3)=COc2c1 ZCOLJUOHXJRHDI-FZHKGVQDSA-N 0.000 description 5
- CJPNHKPXZYYCME-UHFFFAOYSA-N Genistin Natural products OCC1OC(Oc2ccc(O)c3OC(=CC(=O)c23)c4ccc(O)cc4)C(O)C(O)C1O CJPNHKPXZYYCME-UHFFFAOYSA-N 0.000 description 5
- YCUNGEJJOMKCGZ-UHFFFAOYSA-N Pallidiflorin Natural products C1=CC(OC)=CC=C1C1=COC2=CC=CC(O)=C2C1=O YCUNGEJJOMKCGZ-UHFFFAOYSA-N 0.000 description 5
- 150000002212 flavone derivatives Chemical class 0.000 description 5
- ZCOLJUOHXJRHDI-CMWLGVBASA-N genistein 7-O-beta-D-glucoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC(O)=C2C(=O)C(C=3C=CC(O)=CC=3)=COC2=C1 ZCOLJUOHXJRHDI-CMWLGVBASA-N 0.000 description 5
- 230000000968 intestinal effect Effects 0.000 description 5
- NNQSGBRGJHSRFN-UHFFFAOYSA-N isoflavan Chemical compound C1OC2=CC=CC=C2CC1C1=CC=CC=C1 NNQSGBRGJHSRFN-UHFFFAOYSA-N 0.000 description 5
- 235000002324 isoflavanes Nutrition 0.000 description 5
- 244000005700 microbiome Species 0.000 description 5
- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 description 5
- 150000003254 radicals Chemical class 0.000 description 5
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- 235000019158 vitamin B6 Nutrition 0.000 description 5
- 229940011671 vitamin b6 Drugs 0.000 description 5
- 229930003451 Vitamin B1 Natural products 0.000 description 4
- 229930003427 Vitamin E Natural products 0.000 description 4
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- 230000000711 cancerogenic effect Effects 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- -1 flavone compound Chemical class 0.000 description 4
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 4
- 230000036541 health Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 229960003495 thiamine Drugs 0.000 description 4
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 4
- 235000010374 vitamin B1 Nutrition 0.000 description 4
- 239000011691 vitamin B1 Substances 0.000 description 4
- 235000019165 vitamin E Nutrition 0.000 description 4
- 239000011709 vitamin E Substances 0.000 description 4
- 229940046009 vitamin E Drugs 0.000 description 4
- 102000015781 Dietary Proteins Human genes 0.000 description 3
- 238000009825 accumulation Methods 0.000 description 3
- 235000001014 amino acid Nutrition 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 206010012601 diabetes mellitus Diseases 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 210000003608 fece Anatomy 0.000 description 3
- 229930182478 glucoside Natural products 0.000 description 3
- 150000008131 glucosides Chemical class 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- ZUFQODAHGAHPFQ-UHFFFAOYSA-N pyridoxine hydrochloride Chemical compound Cl.CC1=NC=C(CO)C(CO)=C1O ZUFQODAHGAHPFQ-UHFFFAOYSA-N 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 241000186016 Bifidobacterium bifidum Species 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 2
- 206010010774 Constipation Diseases 0.000 description 2
- 206010012735 Diarrhoea Diseases 0.000 description 2
- 108010010256 Dietary Proteins Proteins 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 101710138460 Leaf protein Proteins 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000003110 anti-inflammatory effect Effects 0.000 description 2
- 230000003064 anti-oxidating effect Effects 0.000 description 2
- 230000000259 anti-tumor effect Effects 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 210000000481 breast Anatomy 0.000 description 2
- 231100000357 carcinogen Toxicity 0.000 description 2
- 239000003183 carcinogenic agent Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 235000012000 cholesterol Nutrition 0.000 description 2
- 235000015140 cultured milk Nutrition 0.000 description 2
- 210000002249 digestive system Anatomy 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 239000002552 dosage form Substances 0.000 description 2
- 229930003935 flavonoid Natural products 0.000 description 2
- 150000002215 flavonoids Chemical class 0.000 description 2
- 235000017173 flavonoids Nutrition 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 230000002496 gastric effect Effects 0.000 description 2
- 229910001385 heavy metal Inorganic materials 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 2
- 235000015138 kumis Nutrition 0.000 description 2
- LRDGATPGVJTWLJ-UHFFFAOYSA-N luteolin Natural products OC1=CC(O)=CC(C=2OC3=CC(O)=CC(O)=C3C(=O)C=2)=C1 LRDGATPGVJTWLJ-UHFFFAOYSA-N 0.000 description 2
- 235000009498 luteolin Nutrition 0.000 description 2
- IQPNAANSBPBGFQ-UHFFFAOYSA-N luteolin Chemical compound C=1C(O)=CC(O)=C(C(C=2)=O)C=1OC=2C1=CC=C(O)C(O)=C1 IQPNAANSBPBGFQ-UHFFFAOYSA-N 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 235000021110 pickles Nutrition 0.000 description 2
- 231100000614 poison Toxicity 0.000 description 2
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 229930182490 saponin Natural products 0.000 description 2
- 150000007949 saponins Chemical class 0.000 description 2
- 239000011669 selenium Substances 0.000 description 2
- 229910052711 selenium Inorganic materials 0.000 description 2
- 235000011649 selenium Nutrition 0.000 description 2
- 210000000582 semen Anatomy 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 1
- NWCHELUCVWSRRS-SECBINFHSA-N (2r)-2-hydroxy-2-phenylpropanoic acid Chemical compound OC(=O)[C@@](O)(C)C1=CC=CC=C1 NWCHELUCVWSRRS-SECBINFHSA-N 0.000 description 1
- NWXMGUDVXFXRIG-WESIUVDSSA-N (4s,4as,5as,6s,12ar)-4-(dimethylamino)-1,6,10,11,12a-pentahydroxy-6-methyl-3,12-dioxo-4,4a,5,5a-tetrahydrotetracene-2-carboxamide Chemical compound C1=CC=C2[C@](O)(C)[C@H]3C[C@H]4[C@H](N(C)C)C(=O)C(C(N)=O)=C(O)[C@@]4(O)C(=O)C3=C(O)C2=C1O NWXMGUDVXFXRIG-WESIUVDSSA-N 0.000 description 1
- 206010000087 Abdominal pain upper Diseases 0.000 description 1
- 201000004384 Alopecia Diseases 0.000 description 1
- 229930195573 Amycin Natural products 0.000 description 1
- 206010003011 Appendicitis Diseases 0.000 description 1
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- 241000228197 Aspergillus flavus Species 0.000 description 1
- 241000228230 Aspergillus parasiticus Species 0.000 description 1
- 241001205141 Bifidobacterium animalis subsp. lactis V9 Species 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000050051 Chelone glabra Species 0.000 description 1
- 241001478240 Coccus Species 0.000 description 1
- 206010009900 Colitis ulcerative Diseases 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 206010013554 Diverticulum Diseases 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 208000004232 Enteritis Diseases 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241001646719 Escherichia coli O157:H7 Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 208000031226 Hyperlipidaemia Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 208000008574 Intracranial Hemorrhages Diseases 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 102000010445 Lactoferrin Human genes 0.000 description 1
- 108010063045 Lactoferrin Proteins 0.000 description 1
- 201000010538 Lactose Intolerance Diseases 0.000 description 1
- 241000186781 Listeria Species 0.000 description 1
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 235000010624 Medicago sativa Nutrition 0.000 description 1
- 206010027439 Metal poisoning Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- 240000000064 Penicillium roqueforti Species 0.000 description 1
- 235000002233 Penicillium roqueforti Nutrition 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100039097 Protein IMPACT Human genes 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000293871 Salmonella enterica subsp. enterica serovar Typhi Species 0.000 description 1
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
- 241000607762 Shigella flexneri Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 206010042566 Superinfection Diseases 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 201000006704 Ulcerative Colitis Diseases 0.000 description 1
- 208000006568 Urinary Bladder Calculi Diseases 0.000 description 1
- 206010046996 Varicose vein Diseases 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 235000009392 Vitis Nutrition 0.000 description 1
- 241000219095 Vitis Species 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 208000019790 abdominal distention Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 231100000360 alopecia Toxicity 0.000 description 1
- AEMOLEFTQBMNLQ-BKBMJHBISA-N alpha-D-galacturonic acid Chemical compound O[C@H]1O[C@H](C(O)=O)[C@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-BKBMJHBISA-N 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 229940009289 bifidobacterium lactis Drugs 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 201000008275 breast carcinoma Diseases 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 235000005473 carotenes Nutrition 0.000 description 1
- 150000001746 carotenes Chemical class 0.000 description 1
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 1
- 235000005487 catechin Nutrition 0.000 description 1
- 230000020411 cell activation Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 230000009920 chelation Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 229940099352 cholate Drugs 0.000 description 1
- 201000001883 cholelithiasis Diseases 0.000 description 1
- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 description 1
- 229950001002 cianidanol Drugs 0.000 description 1
- 208000029742 colonic neoplasm Diseases 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 210000004351 coronary vessel Anatomy 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 210000001198 duodenum Anatomy 0.000 description 1
- 208000001848 dysentery Diseases 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000009088 enzymatic function Effects 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000004626 essential fatty acids Nutrition 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 235000021001 fermented dairy product Nutrition 0.000 description 1
- 235000021107 fermented food Nutrition 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 244000005709 gut microbiome Species 0.000 description 1
- 208000010501 heavy metal poisoning Diseases 0.000 description 1
- 208000014617 hemorrhoid Diseases 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 208000002551 irritable bowel syndrome Diseases 0.000 description 1
- 229930013032 isoflavonoid Natural products 0.000 description 1
- 235000012891 isoflavonoids Nutrition 0.000 description 1
- 238000006317 isomerization reaction Methods 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- CSSYQJWUGATIHM-IKGCZBKSSA-N l-phenylalanyl-l-lysyl-l-cysteinyl-l-arginyl-l-arginyl-l-tryptophyl-l-glutaminyl-l-tryptophyl-l-arginyl-l-methionyl-l-lysyl-l-lysyl-l-leucylglycyl-l-alanyl-l-prolyl-l-seryl-l-isoleucyl-l-threonyl-l-cysteinyl-l-valyl-l-arginyl-l-arginyl-l-alanyl-l-phenylal Chemical compound C([C@H](N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CS)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)C1=CC=CC=C1 CSSYQJWUGATIHM-IKGCZBKSSA-N 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 229940078795 lactoferrin Drugs 0.000 description 1
- 235000021242 lactoferrin Nutrition 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 230000037356 lipid metabolism Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000003141 lower extremity Anatomy 0.000 description 1
- 235000012680 lutein Nutrition 0.000 description 1
- 229960005375 lutein Drugs 0.000 description 1
- KBPHJBAIARWVSC-RGZFRNHPSA-N lutein Chemical compound C([C@H](O)CC=1C)C(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\[C@H]1C(C)=C[C@H](O)CC1(C)C KBPHJBAIARWVSC-RGZFRNHPSA-N 0.000 description 1
- ORAKUVXRZWMARG-WZLJTJAWSA-N lutein Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=CC(O)CC2(C)C)C ORAKUVXRZWMARG-WZLJTJAWSA-N 0.000 description 1
- 239000001656 lutein Substances 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 235000020124 milk-based beverage Nutrition 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000004877 mucosa Anatomy 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 210000000578 peripheral nerve Anatomy 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000011321 prophylaxis Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000004223 radioprotective effect Effects 0.000 description 1
- 235000020122 reconstituted milk Nutrition 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 235000017709 saponins Nutrition 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 125000000185 sucrose group Chemical group 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- KBPHJBAIARWVSC-XQIHNALSSA-N trans-lutein Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C(=CC(O)CC2(C)C)C KBPHJBAIARWVSC-XQIHNALSSA-N 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 241000712461 unidentified influenza virus Species 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 208000027185 varicose disease Diseases 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- NCYCYZXNIZJOKI-UHFFFAOYSA-N vitamin A aldehyde Natural products O=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-UHFFFAOYSA-N 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- FJHBOVDFOQMZRV-XQIHNALSSA-N xanthophyll Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C=C(C)C(O)CC2(C)C FJHBOVDFOQMZRV-XQIHNALSSA-N 0.000 description 1
- 235000013618 yogurt Nutrition 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/515—Animalis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/70—Undefined extracts
- C12N2500/76—Undefined extracts from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/245—Lactobacillus casei
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/25—Lactobacillus plantarum
Abstract
The invention provides a composite probiotic-fermented alfalfa sprout powder preparation and a preparation method and application thereof. Tactobacillus plantarum SCI-01, lactobacillus plantarum P-8, bifidobacterium animalis lactis V9 and lactobacillus casei Zhang are taken as fermentation bacteria, and the preservation numbers of the tactobacillus plantarum SCI-01, the lactobacillus plantarum P-8, the bifidobacterium animalis lactis V9 and the lactobacillus casei Zhang are CGMCC No.11930, CGMCC No.6312, CGMCC No.5470 and CGMCC No.5469; a fermentation culture medium is prepared from, by weight, 970-1,000 parts of alfalfa sprout powder, 8-12 parts of sucrose and 8-12 parts of soybean protein powder, and the material-water ratio is 1:1; the initial pH value is 6.0, the total inoculation quantity is 1*10<7> CFU/g, fermentation is performed at 37 DEG C, and fermentation stops when the pH value is 5.0; a fermentation product is subjected to vacuum freeze drying to obtain the probiotic alfalfa raw powder preparation, the live bacterium number of probiotics reaches 1.0*10<10> CFU/g or above, and the probiotic alfalfa raw powder preparation can be prepared into tablets, powder or capsules after being directly mixed with xylitol.
Description
Technical field
The present invention relates to a kind of composite probiotics ferment preparation, particularly relate to a kind of by Lactobacillus plantarum SCI-01, lactobacillus casei
Herba Medicaginis tender shoots powder preparation of Zhang, Bifidobacterium lactis V9 and Lactobacillus plantarum P-8 mixing fermentation and its preparation method and application,
Belong to probiotics fermention preparation technique field.
Background technology
Probiotic bacteria is that a class produces the microorganism of beneficial effect by improving enteric microorganism to host.It can be by participating in intestinal
Road microbial balance suppresses growth of pathogenic bacteria, it is to avoid drug resistance that life-time service antibiotic produces and superinfection, therefore,
Probiotic products enjoys the concern of people in recent years.At present, although the probiotic products on market is the abundantest, but exploitation
Product, based on fermented dairy product, wherein contains high metabolism of lipid and cholesterol, it is also possible to cause lactose intolerance.So, non-breast
Probiotic products becomes the trend of development.And leguminous plant Herba Medicaginis, having the highest nutrition and health care is worth, natural as one
Base-forming food, containing substantial amounts of alfalfa leaf protein, dietary fiber, active polysaccharide, Saponin, flavone, vitamin E, selenium, leaf is yellow
Active skull cap components, its alimentary health-care function and the contained physiologically actives thereof such as element have become nourishing healthy and have extracted the preciousness of medicine
Resource.So, the fermented food with Herba Medicaginis as substrate will increasingly be paid close attention to by people.
The leaf protein of Alfalfa tender shoots contains 18 kinds of aminoacid, including 8 kinds of essential amino acids, and proportion of composing equilibrium, with connection
Adult's amino acid pattern that He Guo food and agricultural organization is recommended is consistent substantially, particularly lysine, and lutein content is the highest, and rich in
Substantial amounts of carotene, essential fatty acid, multivitamin, and selenium, the mineral element such as ferrum, magnesium, calcium, zinc, copper,
Have prevent and cure diseases, health care is anti-ageing, vigorous body, the different physiological roles such as content significantly improving blood in human body in Lactoferrin.
The dietary fiber of Alfalfa tender shoots mainly includes cellulose, hemicellulose, pectin and pectic substance and four portions of lignin
Point.Dietary fiber has important physiological function, including: dietary fiber can reduce feces stop in conjunction with the carcinogen in enteric cavity
Stay the time, and then reduce contacting of colorectal mucosa and carcinogen, make feces deliquescing and increase the output of feces, can prevent just
Secret, colon cancer, intestina diverticulum, hemorrhoid and varicose veins of the lower extremity；Reduce serum cholesterol, prevent the heart caused by coronary arteries hardening
Disease of ZANG-organs；Improve the peripheral nerve tissue susceptibility to insulin, the blood sugar level of diabetes patient can be regulated；It is that a kind of noenergy is filled out
Charge, can prevent and treat obesity；The shortage of dietary fiber or deficiency are also burst with appendicitis, cholelithiasis, vesical calculus, duodenum
The sickness rate of the diseases such as infections, ulcerative colitis and breast carcinoma and occurring degree have the biggest relation.
The vegetable polysaccharides of Alfalfa tender shoots especially, after isomerization or modification, has good antitumor action, to T cell, B
The immunity of cell activation has potentiation, it is also possible to blood fat reducing, blood sugar lowering, removing toxic substances, antibacterial, radioprotective.Alfalfa Polysaccharide can
Improve body lymphocyte and produce the ability of antibody.
The flavone compound of Alfalfa tender shoots is one of principle active component of Chinese herbal medicine, and (1) catechin can promote the life of animal
Long, increase the function of enzyme in cell, (2) antioxidation and the effect of removing free radical, (3) heavy metal has chelation, because of
This people of life and work in Heavy-metal Polluted Environment takes flavone compound and can prevent heavy metal poisoning；(4) biological flavone has
Effect to antitumor cell overgrowing, and cancerigenic factor and carcinogenic factor are had biochemical inhibitory action.The generation of tumor can be prevented
With suppression tumor growth；(5) flavone in Herba Medicaginis is to make vitamin C stable and be not easy the most important material being destroyed.Yellow
Ketone can also be strengthened ascorbic antioxidation and remove the effect of free radical.(6) biological flavone is microbial inhibitor, to Fructus Vitis viniferae
Coccus, escherichia coli, dysentery bacterium and Bacillus typhi have inhibitory action, have virus (such as influenza virus, enteritis virus) very
Strong infection and antiinflammatory action.
The vitamin E of Alfalfa tender shoots is all widely used on medicine, cosmetics.Vitamin E is a kind of antioxidant, can
With the destruction to antioxidant radical, suppressing the formation of poisonous lipid peroxide, protection cell membrane does not destroys, and makes unsaturation
Fatty acid is stable, maintains and organizes normal metabolism, has a wide range of applications the most clinically.As increase body's immunity,
Delaying human body caducity, prevent and treat cardiovascular disease, treat arteriosclerosis and the alopecia preventing amycin from causing, prevention brain
Hemorrhage and diabetes etc..Vitamin E can enter cell, and has the effect of free radical resisting chain reaction, so at skin
In application in maintenance the most promising.
The soaping agents of Alfalfa tender shoots has diversified pharmacologically active and biological activity, as cholesterol can be suppressed in the suction of intestinal
Receive, reduce plasma cholesterol, preventing and treating cardiovascular and cerebrovascular disease, blood pressure lowering, antibacterial, antiinflammatory, reduction of heat, calmness, the aspect such as strong
Pharmacological action.Alfalfa saponin toxicity is low, medicine source is many, develops into useful blood fat reducing and the medicine for the treatment of coronary heart disease, is to have very much valency
Value.
The present invention utilizes and is individually separated the L.plantarum in Pickles, cultured milk, healthy children intestinal and koumiss sample
SCI-01, L.plantarum P-8, B.lactis V9 and L.casei Zhang probiotic strain fermentation Herba Medicaginis tender shoots powder.In prior art
The many fermentations of alfalfa are applied to ensilage, not yet have this type of composite probiotics ferment Herba Medicaginis tender shoots powder preparation.
Patent application CN102813004A discloses the soyabean milk beverage prepared with lactobacillus casei and bacillus bifidus for leaven, its
Feature is that composition is: Soybean Milk or Semen sojae atricolor powder reconstituted milk 300kg, glucose 10～15kg, sucrose 100～120kg are stably
Agent 2～4kg, acid solution 0.1～0.3kg, edible essence or spice 0.1～0.3kg, lactobacillus casei (Lactobacillus casei Zhang)
Leaven 0.015～0.03kg, bacillus bifidus (Bifidobacterium animalis subsp.lactis V9) leaven 0.015～
0.03kg, described leaven bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's General Microbiological Culture preservation center,
Preserving number is respectively CGMCCNo.5469 and CGMCC No.5470.
Patent application CN102994422A discloses a kind of Lactobacillus plantarum, it is characterised in that: described Lactobacillus plantarum
(Lactobacillus plantarum P-8) strains separation, in clabber, has the anti-gastrointestinal tract Digestive system tolerance of excellence.
This bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number: CGMCC No.6312.
Summary of the invention
It is an object of the present invention to provide a kind of composite probiotics ferment alfalfa meal preparation, utilization is individually separated from Pickles, cultured milk, is good for
L.plantarum SCI-01 in health child's intestinal and koumiss sample, L.plantarum P-8, B.lactis V9 and L.casei
Zhang probiotic strain fermentation Herba Medicaginis tender shoots powder, above-mentioned probiotic bacteria has the anti-gastrointestinal tract Digestive system tolerance of excellence；Improve people
The ability of body intestinal microflora；Regulation blood lipid metabolism, has Conservative restoration effect, enhancing body antioxygen to hyperlipidemia liver
Change ability；Immunity of organism is had regulation effect；The irritable bowel syndromes such as diarrhoea, constipation, stomachache, abdominal distention are alleviated in effectively prevention；
Effectively prophylaxis of tumours grows the effects such as the generation with type Ⅱdiabetes mellitus.Utilize above-mentioned composite probiotics ferment Herba Medicaginis tender shoots powder, not only
Nutritive value and the mouthfeel of Herba Medicaginis can be improved, moreover it is possible to form probiotic bacteria and add the health-care effect of prebiotics.
It is an object of the invention to be achieved through the following technical solutions:
A kind of composite probiotics ferment Herba Medicaginis tender shoots powder preparation, with Lactobacillus plantarum SCI-01 (L.plantarum SCI-01), plants
Thing lactobacillus P-8 (L.plantarum P-8), bifidobacterium animalis subspecies V9 (B.lactis V9) and Lactobacillus casei Zhang
(L.casei Zhang) is fermenting agent, its deposit number be respectively CGMCC No.11930, CGMCC No.6312,
CGMCC No.5470 and CGMCC No.5469；Fermentation medium forms: in terms of parts by weight, Herba Medicaginis tender shoots powder 970-1000,
Sucrose 8-12, soybean protein powder 8-12, material-water ratio 1:1；Initial pH 6.0, inoculates above-mentioned four kinds of microbial inoculums, and total inoculum concentration is
1×107CFU/g, 37 DEG C of fermentations, pH 5.0 is fermentation termination.
Preferably, described fermenting agent L.plantarum SCI-01, L.plantarum P-8, B.lactis V9 and L.casei Zhang
Inoculum concentration ratio be 1:1:1:2.
1.0 × 10 are reached by the number of live bacteria of probiotics in above-mentioned gained probiotic bacteria Herba Medicaginis tunning10More than CFU/g.
Described probiotic bacteria Herba Medicaginis tunning mix homogeneously according to the ratio of 1:2 with the protective agent after aseptic process after through vacuum freezing
Being dried the preparation former powder preparation of Herba Medicaginis probiotic bacteria, in this former powder preparation of Herba Medicaginis probiotic bacteria, number of live bacteria of probiotics reaches 1.0 × 1010CFU/g with
On.
Preferably, described protective agent consists of: trehalose 4%, lactose 4%, soybean protein powder 2%, distilled water 90%.)
After the described former powder preparation of Herba Medicaginis probiotic bacteria directly or is allocated with xylitol etc., can be prepared as Herba Medicaginis probiotic tablet, powder
The oral formulations of the common dosage forms such as agent, capsule.These preparations have that easy to carry and use, probiotic bacteria content is high, are prone to disappear
Change good absorbing, feature in good taste.In preparation after formulated, number of live bacteria of probiotics is not less than 2.0 × 109CFU/g。
The present invention provides a kind of Lactobacillus plantarum L.plantarum SCI-01, within 24th, is preserved in Chinese micro-life in December in 2015
(abbreviation CGMCC address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, thing culture presevation administration committee's common micro-organisms center
No. 3, Institute of Microorganism, Academia Sinica, postcode: 100101), deposit number: CGMCC No.11930, Classification And Nomenclature:
Lactobacillus plantarum Lactobacillus plantarum.
The Lactobacillus plantarum L.plantarum SCI-01 that the present invention provides is to filter out to suppress intestinal in strains of lactic acid bacteria more than 300
Road malignant bacteria is (Escherichia coli O 157: H7, Salmonella typhimurium, shigella flexneri, staphylococcus aureus, slender
Born of the same parents' listeria spp) and the Lactic Acid Bacteria strain of rot fungi (Aspergillus flavus, aspergillus parasiticus, penicillium roqueforti), this bacterial strain can be high
Produce broad-spectrum antibacterial material phenyllactic acid (70mg/L).Find that its bacteriostatic activity strengthens and has protease good by compounding fermentation
Good stability and bacterial strain have good tolerance gastro-intestinal Fluid and the feature of cholate.Bacterial strain L.plantarum SCI-01 has latent
At probiotic properties, application can be developed as the biological preservation bacterium in Yoghourt.
Beneficial effect:
Four bacterium combination (L.casei Zhang, B.animalis V9, L.plantarum P-8, L.plantarum SCI-01), wherein
L.casei Zhang, B.animalis V9 and L.plantarum P-8 meet the gastrointestinal characteristics of Chinese oneself；Have blood fat reducing,
Pre-anti-diarrhea, constipation；Prevention diabetes；Improve the probiotic properties of intestine microenvironment.Prepared Herba Medicaginis probiotics preparation
There is probiotic bacteria content high (active probiotic 200,000,000,000/100g), the feature that in shelf life, viable bacteria vigor is high；Herba Medicaginis tender shoots powder is through 4
After probiotics fermentation, its organic acid, vitamin, free amino acid, biologically active peptide increase, and active isoflavan content increases,
It is prone to human consumption absorb and the lifting of health efficacy.
In composite probiotics ferment Herba Medicaginis tender shoots powder, vitamin B1 and B6 content have raising in various degree.VB1 after fermentation
Content is 5.69ug/g, relatively improves 65.4% before fermentation；VB6 content increases notable, and after fermentation, content is 24.59ug/g.Lactic acid
Bacterium fermentation Herba Medicaginis tender shoots powder is conducive to the accumulation of vitamin B6 and B1.Composite probiotics ferment Herba Medicaginis tender shoots powder, it is possible to affect lucerne
Original isoflavone content in Mu.Glucoside type isoflavone (genistin) content that after fermentation, activity is more weak reduces by 30.7%, for
3.88ug/g, and the content of daidzein increases after fermentation, for 0.44ug/g, content improves 76.0% before relatively fermenting.Compound
Probiotics fermention Herba Medicaginis is conducive to the increase of active isoflavan content (daidzein), the more conducively human body absorption to isoflavone.
Accompanying drawing explanation
Fig. 1: the different sterilization method impacts on Herba Medicaginis tender shoots powder miscellaneous bacteria；
Fig. 2: soybean protein can promote the compound probiotic fermentation to Herba Medicaginis tender shoots powder；
Fig. 3: add sucrose and can significantly carry Herba Medicaginis tender shoots powder probiotic bacteria content of feeling embarrassed；
The impact on fermentation alfalfa meal probiotic bacteria content of Fig. 4: the different vaccination ratio；
Fig. 5: composite probiotics ferment can dramatically increase the content of Herba Medicaginis vitamin B group；
Fig. 6: composite probiotics ferment can dramatically increase the content of active isoflavan in Herba Medicaginis；Wherein, 6 (a) is dyewood
Glycosides changes of contents, 6 (b) is daidzein changes of contents；
Fig. 7: protective agent can significantly improve the probiotic bacteria survival rate in fermentation alfalfa meal freeze-drying process.
Detailed description of the invention
Below in conjunction with specific embodiment further describe the present invention, advantages of the present invention and feature will be with describe and apparent.
But these examples are only exemplary, the scope of the present invention is not constituted any restriction.Those skilled in the art should understand that
It is the details of technical solution of the present invention and form can be modified or replace lower without departing from the spirit and scope of the present invention,
But these amendments and replacement each fall within protection scope of the present invention.Method, composition and the consumption related in following embodiment, as
Without specified otherwise, conventional method the most known to those skilled in the art.
The probiotics fermention microbial inoculum used in following embodiment is active freeze-dried mycopowder.
Compound probiotic (L.plantarum SCI-01, L.plantarum P-8, B.lactis V9 and L.casei Zhang) ferments
The preparation method of Herba Medicaginis tender shoots powder preparation.
Herba Medicaginis tender shoots powder is mixed in proportion rear sterilization treatment with soybean protein powder, cane sugar powder and distilled water.L.plantarum
SCI-01, L.plantarum P-8, B.lactis V9 and L.casei Zhang fermenting agent is inoculated in the fermentation culture that sterilizing is complete
In base, 6.0,37 DEG C of constant temperature culture of initial pH, pH5.0 is fermentation termination.
Count plate:
General employing pour plate counting method, operating procedure is: according to national standard method, shakes up fermentation liquid, draws 1mL fermentation liquid
It is placed in the physiological saline solution of 9mL, carries out 10 times of dilutions, be diluted to required gradient the most successively.To finally dilute gradient
Tube dilution shake even after, draw 1mL diluent pour into flat board, to entering culture medium, shake up, put solidification, in 37 DEG C of cultivations
48h cultivated by case.Flat board does two gradients respectively, and each gradient two is parallel.
Mensuration viable count of lactobacillus culture medium: MRS solid medium, Anaerobic culturel.
PH measures: use accurate pH meter directly to measure fermentation liquid pH value.
Embodiment 1:
The different sterilization method impact on Herba Medicaginis fermentation medium of research.
Solid fermentation is different from liquid, has that heat transfer rate is slow, it is uneven to conduct heat, the halfway difficult point of sterilizing.Research difference is gone out
Bacterium mode is to the killing effect of miscellaneous bacteria in Herba Medicaginis tender shoots powder.Concrete outcome is shown in Fig. 1.The raising of temperature, original miscellaneous in alfalfa meal
Bacterium is gradually lowered, and when sterilising conditions is 121 DEG C of 15min, sterilization effect is best；In addition compared to antibacterial (predominantly spore bar
Bacterium), fungus thermostability is relatively low, and 95 DEG C of 30min i.e. can reach sterilization effect.The most as shown in Figure 1.
Embodiment 2:
The research soybean protein impact on composite probiotics ferment Herba Medicaginis tender shoots powder.
Inoculum concentration is identical with each microbial inoculum ratio.
A. fermentation medium: Herba Medicaginis tender shoots powder 1000g, sucrose 0g, soybean protein powder 0g, material-water ratio 1:1.Fermentation alfalfa meal
Middle viable count of lactobacillus is 4.93 × 109CFU/g。
B. fermentation medium: Herba Medicaginis tender shoots powder 1000g, sucrose 0g, soybean protein powder 10g, material-water ratio 1:1.Fermentation alfalfa meal
Middle viable count of lactobacillus is 7.09 × 109CFU/g。
Result of the test shows: the Herba Medicaginis tender shoots powder processed through albumen, the beneficially growth of lactic acid bacteria.After albumen processes, fermentation
The viable count of lactobacillus of Herba Medicaginis is 70.9 hundred million CFU/g, and more untreated viable count improves 43.9%.The most as shown in Figure 2.
Embodiment 3:
The research sucrose impact on probiotics fermention Herba Medicaginis tender shoots powder.
Inoculum concentration is identical with each microbial inoculum ratio.
A. Herba Medicaginis tender shoots powder 1000g, sucrose 0g, soybean protein powder 10g, material-water ratio 1:1.Viable lactic acid bacteria in fermentation alfalfa meal
Number is 6.75 × 109CFU/g。
B. Herba Medicaginis tender shoots powder 1000g, sucrose 10g, soybean protein powder 10g, material-water ratio 1:1.In fermentation alfalfa meal, lactic acid bacteria is lived
Bacterium number is 1.015 × 1010CFU/g。
C. Herba Medicaginis tender shoots powder 1000g, sucrose 20g, soybean protein powder 10g, material-water ratio 1:1.In fermentation alfalfa meal, lactic acid bacteria is lived
Bacterium number is 1.102 × 1010CFU/g。
Result of the test shows: add the Herba Medicaginis tender shoots powder of sucrose, the beneficially growth of lactic acid bacteria.After interpolation, the breast of fermentation Herba Medicaginis
Acid bacterium viable count is 101.5～110.2 hundred million CFU/g, is relatively not added with sucrose group viable count and improves 50.37～63.26%.It is specifically shown in figure
Shown in 3.
Embodiment 4:
The research different strain inoculative proportion impact on Herba Medicaginis fermentation medium.
Fermentation culture and composition: in terms of parts by weight, Herba Medicaginis tender shoots powder 970-1000, sucrose 8-12, soybean protein powder 8-12,
Material-water ratio 1:1.
A. when total inoculum concentration of L.plantarum SCI-01, L.plantarum P-8, B.lactis V9 and L.casei Zhang it is
1×107CFU/g (inoculative proportion 1:1:1:1), 37 DEG C of fermentations, measuring viable count of lactobacillus is 0.72 × 1010CFU/g。
B. when total inoculum concentration of L.plantarum SCI-01, L.plantarum P-8, B.lactis V9 and L.casei Zhang it is
1×107CFU/g (inoculative proportion 1:2:1:1), 37 DEG C of fermentations, measuring viable count of lactobacillus is 0.84 × 1010CFU/g。
Total inoculum concentration of c.L.plantarum SCI-01, L.plantarum P-8, B.lactis V9 and L.casei Zhang is 1 × 107
CFU/g (inoculative proportion 1:1:2:1), 37 DEG C of fermentations, measuring viable count of lactobacillus is 0.93 × 1010CFU/g。
Total inoculum concentration of d.L.plantarum SCI-01, L.plantarum P-8, B.lactis V9 and L.casei Zhang is 1 × 107
CFU/g (inoculative proportion 1:1:1:2), 37 DEG C of fermentations, measuring viable count of lactobacillus is 1.03 × 1010CFU/g。
Experimental result: according to experimental result, as L.plantarum SCI-01, L.plantarum P-8, B.lactis V9 and L.casei
Total inoculum concentration of Zhang is 1 × 107CFU/g (inoculative proportion 1:1:1:2), is more beneficial for the fermentation of alfalfa meal.It is specifically shown in Fig. 4 institute
Show.
Summary is studied, and the Optimal compositions of fermentation medium of composite probiotics ferment alfalfa meal consists of: in terms of parts by weight, Herba Medicaginis
Tender shoots powder 970-1000, sucrose 8-12, soybean protein powder 8-12, material-water ratio 1:1.Initial pH6.0, inoculates L.plantarum
SCI-01, L.plantarum P-8, B.lactis V9 and L.casei Zhang fermenting agent, inoculative proportion is 1:1:1:2, always connects
The amount of kind is 1 × 107CFU/g, 37 DEG C of fermentations, pH 5.0 is fermentation termination.In tunning, number of live bacteria of probiotics reaches 1.0 × 1010
More than CFU/g.Now, in composite probiotics ferment Herba Medicaginis tender shoots powder, vitamin B1 and B6 content have raising in various degree.
After fermentation, VB1 content is 5.69ug/g, relatively improves 65.4% before fermentation；VB6 content increases notable, and after fermentation, content is
24.59ug/g.Lactic acid bacteria fermentation Herba Medicaginis tender shoots powder is conducive to the accumulation of vitamin B6 and B1.Composite probiotics ferment Herba Medicaginis tender shoots
Powder, it is possible to affect original isoflavone content in Herba Medicaginis.After fermentation, more weak glucoside type isoflavone (genistin) of activity contains
Amount reduction by 30.7%, for 3.88ug/g, and the content of daidzein increases after fermentation, and for 0.44ug/g, content relatively ferments premise
High by 76.0%.Composite probiotics ferment Herba Medicaginis is conducive to the increase of active isoflavan content (daidzein), more conducively human body
Absorption to isoflavone.Concrete outcome is shown in Fig. 5-6.
Embodiment 5:
The different Protective substances impact on lyophilization Herba Medicaginis probiotics preparation of research.
A. the protective agent (distilled water) after described compound probiotic Herba Medicaginis tunning and aseptic process mixes according to the ratio of 1:2
Preparing the former powder preparation of Herba Medicaginis probiotic bacteria through vacuum lyophilization after closing uniformly, in said preparation, number of live bacteria of probiotics reaches 5.9 × 109
CFU/g。
B. protective agent (trehalose 4%, lactose 4%, the Semen sojae atricolor after described compound probiotic Herba Medicaginis tunning and aseptic process
Egg albumen powder 2%, distilled water 90%) prepare the former powder system of Herba Medicaginis probiotic bacteria according to after the ratio mix homogeneously of 1:2 through vacuum lyophilization
Agent, in said preparation, number of live bacteria of probiotics reaches 1.0 × 1010CFU/g。
Experimental result: according to experimental result, the protective agent (trehalose after compound probiotic Herba Medicaginis tunning with aseptic process
4%, lactose 4%, soybean protein powder 2%) according to after the ratio mix homogeneously of 1:2 through vacuum lyophilization, beneficially Herba Medicaginis preparation
The preservation of middle probiotic active and raising.The most as shown in Figure 7.
Embodiment 6:
The application of composite probiotics ferment alfalfa meal preparation.
Probiotics fermention Herba Medicaginis dry powder after vacuum lyophilization is prepared Herba Medicaginis probiotic bacteria after low-temperature grinding crosses 100 mesh sieves
Former powder.After the former powder of Herba Medicaginis probiotic bacteria directly or is allocated with xylitol etc., can be prepared as Herba Medicaginis probiotic tablet, powder, capsule
Oral formulations Deng common dosage forms.
Herba Medicaginis probiotic bacteria former powder 70-90 part, xylitol 10-30 part is extruded for 0.5-0.8g tablet through tablet machine.Benefit in tablet
Raw bacterial content is for being not less than 2,000,000,000 CFU/g.
Herba Medicaginis probiotic bacteria former powder 70-90 part, xylitol 10-30 part is packaged as the powder into 2.0-2.5g through powder packing machine.Powder
Middle probiotic bacteria content is for being not less than 7,000,000,000 CFU/g.
Herba Medicaginis probiotic bacteria former powder 70-90 part, xylitol 10-30 part is filled to the capsule of 0.2-0.3g through capsule filler.Capsule
Middle probiotic bacteria content is for being not less than 7,000,000,000 CFU/g.
After storage test finds that Herba Medicaginis probiotic tablet was in 4 DEG C and 25 DEG C storages 1 month, its probiotic bacteria survival rate is respectively 95%
With 60%, concrete storage data are shown in Table 1.After Herba Medicaginis probiotic bacteria powder or capsule 4 DEG C and 25 DEG C are preserved 1 month, its probiotic bacteria
Survival rate is respectively 97% and 75%, and concrete storage data are shown in Table 2.
Herba Medicaginis probiotic tablet, powder and capsule rich in proteins, dietary fiber, additionally contain the flavonoid useful to human body
Material (luteolin), isoflavonoid compounds (daidzein, genistin), be specifically shown in Table 3.
Table 1 Herba Medicaginis probiotic tablet storage-stable result
Table 2 Herba Medicaginis probiotic bacteria powder, capsule preparations storage-stable result
Table 3 Herba Medicaginis probiotic tablet nutritional labeling
| Project | Every 100 grams (g) |
| Protein | 18.43g |
| Fat | 6.59g |
| Carbohydrate | 34.42g |
| Sodium | 239mg |
| Potassium | 3.69g |
| Dietary fiber | 28.70g |
| Vitamin B6 | 0.3mg |
| Flavone (luteolin) | 0.61mg |
| Isoflavone (daidzein) | 0.05mg |
| Isoflavone (genistin) | 0.12mg |
After the former powder of compound probiotic Herba Medicaginis lyophilizing directly or is allocated with xylitol etc., it is shaped in tablet, powder and capsule preparations benefit
Raw bacterium viable count is not less than 2.0 × 109CFU/g.Herba Medicaginis probiotic tablet, powder and capsule are easy to carry and use, probiotic bacteria contains
Amount is high, mouthfeel is obviously improved and storage-stable is good.The nutrient substance such as rich in proteins, dietary fiber, additionally contains people
Flavonoid that body is useful, isoflavone like substance.L.plantarum SCI-01, L.plantarum P-8, B.lactis V9 and L.casei
Zhang fermentation Herba Medicaginis tender shoots powder is conducive to vitamin B6 and the accumulation of B1 and the raising of active isoflavan content.VB1 after fermentation
Content improves 65.4% before relatively fermenting；Glucoside type isoflavone (genistin) content that after fermentation, activity is more weak reduces by 30.7%,
And the content of daidzein improves 76.0% before relatively fermenting.
Claims (8)
1. a composite probiotics ferment Herba Medicaginis tender shoots powder preparation, it is characterised in that with Lactobacillus plantarum SCI-01, Lactobacillus plantarum
P-8, bifidobacterium animalis subspecies V9 and Lactobacillus casei Zhang are fermenting agent, and its deposit number is respectively CGMCC
No.11930, CGMCC No.6312, CGMCC No.5470 and CGMCC No.5469；Fermentation medium forms:
In terms of parts by weight, Herba Medicaginis tender shoots powder 970-1000, sucrose 8-12, soybean protein powder 8-12, material-water ratio 1:1；Initially
PH 6.0, inoculates above-mentioned four kinds of microbial inoculums, and total inoculum concentration is 1 × 107CFU/g, 37 DEG C of fermentations, pH 5.0 is fermentation termination；
Tunning obtains composite probiotics ferment Herba Medicaginis tender shoots powder preparation through vacuum lyophilization.
2. composite probiotics ferment Herba Medicaginis tender shoots powder preparation as claimed in claim 1, it is characterised in that described fermenting agent plant
Lactobacillus SCI-01, Lactobacillus plantarum P-8, bifidobacterium animalis subspecies V9 and the inoculum concentration of Lactobacillus casei Zhang
Ratio is 1:1:1:2.
3. composite probiotics ferment Herba Medicaginis tender shoots powder preparation as claimed in claim 1, it is characterised in that described tunning and guarantor
Protect agent and obtain composite probiotics ferment Herba Medicaginis tender shoots powder preparation according to after the ratio mix homogeneously of 1:2 through vacuum lyophilization.
4. composite probiotics ferment Herba Medicaginis tender shoots powder preparation as claimed in claim 3, it is characterised in that described protectant composition
For: trehalose 4%, lactose 4%, soybean protein powder 2%, distilled water 90%.
5. the composite probiotics ferment Herba Medicaginis tender shoots powder preparation as described in claim 2 or 4, it is characterised in that described tunning
Or the number of live bacteria of probiotics in composite probiotics ferment Herba Medicaginis tender shoots powder preparation reaches 1.0 × 1010More than CFU/g.
6. the purposes of the arbitrary described composite probiotics ferment Herba Medicaginis tender shoots powder preparation of claim 1-5, it is characterised in that described
After the former powder preparation of Herba Medicaginis probiotic bacteria directly or is allocated with xylitol, it is prepared as Herba Medicaginis probiotic tablet, powder or capsule.
7. the purposes of composite probiotics ferment Herba Medicaginis tender shoots powder preparation described in claim 6, it is characterised in that the Herba Medicaginis after formulated
Number of live bacteria of probiotics in probiotic tablet, powder or capsule is not less than 2.0 × 109CFU/g。
8. the preparation method of a composite probiotics ferment Herba Medicaginis tender shoots powder preparation, it is characterised in that with Lactobacillus plantarum SCI-01,
Lactobacillus plantarum P-8, bifidobacterium animalis subspecies V9 and Lactobacillus casei Zhang are fermenting agent, its deposit number
It is respectively CGMCC No.11930, CGMCC No.6312, CGMCC No.5470 and CGMCC No.5469；Send out
Ferment culture medium forms: in terms of parts by weight, Herba Medicaginis tender shoots powder 970-1000, sucrose 8-12, soybean protein powder 8-12, material
Water compares 1:1；Initial pH 6.0, inoculates above-mentioned four kinds of microbial inoculums according to the ratio of 1:1:1:2, and total inoculum concentration is 1 × 107CFU/g,
37 DEG C of fermentations, pH 5.0 is fermentation termination；Tunning is cold through vacuum after mixing homogeneously according to the ratio of 1:2 with protective agent
The lyophilizing dry acquisition former powder preparation of Herba Medicaginis probiotic bacteria；Described protective agent is: trehalose 4%, lactose 4%, soybean protein powder 2%,
Distilled water 90%；Number of live bacteria of probiotics in described composite probiotics ferment Herba Medicaginis tender shoots powder preparation reaches 1.0 × 1010
More than CFU/g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610171344.8A CN105802876B (en) | 2016-03-24 | 2016-03-24 | A kind of composite probiotics ferment clover tender shoots powder preparation and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610171344.8A CN105802876B (en) | 2016-03-24 | 2016-03-24 | A kind of composite probiotics ferment clover tender shoots powder preparation and its preparation method and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105802876A true CN105802876A (en) | 2016-07-27 |
| CN105802876B CN105802876B (en) | 2019-05-10 |
Family
ID=56454852
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610171344.8A Active CN105802876B (en) | 2016-03-24 | 2016-03-24 | A kind of composite probiotics ferment clover tender shoots powder preparation and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105802876B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107988115A (en) * | 2017-12-27 | 2018-05-04 | 北京科拓恒通生物技术股份有限公司 | Lactobacillus plantarum and its composite probiotics ferment liquid and preparation method |
| CN108113001A (en) * | 2017-12-28 | 2018-06-05 | 福建拓天生物科技有限公司 | A kind of Freeze-dry Powder of Probioctics for treating gastric ulcer |
| CN112741210A (en) * | 2020-12-17 | 2021-05-04 | 湖北绿天地生物科技有限公司 | Biological preparation for improving animal organism immunity function and preparation method thereof |
| CN116716228A (en) * | 2023-07-24 | 2023-09-08 | 中国农业大学 | Lactobacillus plantarum EL6 with flavonoid content improving function and application thereof |
| CN116716228B (en) * | 2023-07-24 | 2024-04-19 | 中国农业大学 | Lactobacillus plantarum EL6 with flavonoid content improving function and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101735964A (en) * | 2008-11-18 | 2010-06-16 | 中国农业大学 | Microbial inoculum for preparing feed and application thereof |
| CN102138582A (en) * | 2010-12-27 | 2011-08-03 | 内蒙古伊利实业集团股份有限公司 | Lactobacillus casei-containing active lactobacillus drink and preparation method thereof |
| CN102813004A (en) * | 2012-01-10 | 2012-12-12 | 北京和美科盛生物技术有限公司 | Soybean milk beverage prepared through using Lactobacillus casei Zhang and Bifidobacterium animalis subsp.lactis V9, and its preparation method |
| CN102994422A (en) * | 2012-11-12 | 2013-03-27 | 北京和美科健生物技术有限责任公司 | Application of lactobacillus plantarum P-8 in improvement of alcoholic liver injury |
| CN104845904A (en) * | 2015-02-27 | 2015-08-19 | 河北科技大学 | Lactobacillus plantarum bacterial strain and application thereof |
| CN105104909A (en) * | 2015-09-24 | 2015-12-02 | 广州金水动物保健品有限公司 | Silage and preparation method and application thereof |
-
2016
- 2016-03-24 CN CN201610171344.8A patent/CN105802876B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101735964A (en) * | 2008-11-18 | 2010-06-16 | 中国农业大学 | Microbial inoculum for preparing feed and application thereof |
| CN102138582A (en) * | 2010-12-27 | 2011-08-03 | 内蒙古伊利实业集团股份有限公司 | Lactobacillus casei-containing active lactobacillus drink and preparation method thereof |
| CN102813004A (en) * | 2012-01-10 | 2012-12-12 | 北京和美科盛生物技术有限公司 | Soybean milk beverage prepared through using Lactobacillus casei Zhang and Bifidobacterium animalis subsp.lactis V9, and its preparation method |
| CN102994422A (en) * | 2012-11-12 | 2013-03-27 | 北京和美科健生物技术有限责任公司 | Application of lactobacillus plantarum P-8 in improvement of alcoholic liver injury |
| CN104845904A (en) * | 2015-02-27 | 2015-08-19 | 河北科技大学 | Lactobacillus plantarum bacterial strain and application thereof |
| CN105104909A (en) * | 2015-09-24 | 2015-12-02 | 广州金水动物保健品有限公司 | Silage and preparation method and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| 张和义等: "《芽苗菜生产新技术》", 31 January 2014, 金盾出版社 * |
| 张莉力等: "产苯乳酸乳酸菌的筛选及鉴定", 《安徽农业科学》 * |
| 潘卫仓: "紫花苜蓿的主要成分及其在畜牧业中的应用", 《中兽医医药杂志》 * |
| 高玉荣等: "《新型功能性大豆发酵食品》", 31 December 2015, 中国纺织出版社 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107988115A (en) * | 2017-12-27 | 2018-05-04 | 北京科拓恒通生物技术股份有限公司 | Lactobacillus plantarum and its composite probiotics ferment liquid and preparation method |
| CN108113001A (en) * | 2017-12-28 | 2018-06-05 | 福建拓天生物科技有限公司 | A kind of Freeze-dry Powder of Probioctics for treating gastric ulcer |
| CN108113001B (en) * | 2017-12-28 | 2021-03-30 | 福建拓天生物科技有限公司 | Probiotic freeze-dried powder for relieving gastric ulcer |
| CN112741210A (en) * | 2020-12-17 | 2021-05-04 | 湖北绿天地生物科技有限公司 | Biological preparation for improving animal organism immunity function and preparation method thereof |
| CN116716228A (en) * | 2023-07-24 | 2023-09-08 | 中国农业大学 | Lactobacillus plantarum EL6 with flavonoid content improving function and application thereof |
| CN116716228B (en) * | 2023-07-24 | 2024-04-19 | 中国农业大学 | Lactobacillus plantarum EL6 with flavonoid content improving function and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105802876B (en) | 2019-05-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105146614B (en) | A kind of functional calcium fruit ferment, enzyme beverage and its production method | |
| CA3010577A1 (en) | A composition comprising a lactic acid bacteria for preventing and treating vaginosis and the use thereof | |
| CN106173773A (en) | A kind of ferment probiotic bacteria solid beverage and preparation method thereof | |
| CN104770816A (en) | Watermelon juice probiotic fermented beverage and preparation method thereof | |
| CN109123295A (en) | A kind of probiotics solid beverage and preparation method thereof | |
| CN102215848A (en) | Lactobacillus rhamnosus and weight control | |
| CN101257910A (en) | Cholesterol absorption inhibitor | |
| CN107981324A (en) | Turmeric fermented food and preparation method thereof | |
| CN104757112B (en) | A kind of special flavor functional yoghourt and preparation method thereof | |
| CN109628358A (en) | A kind of compound probiotic and its application | |
| CN110338402A (en) | A kind of compound probiotic powder and preparation method thereof | |
| CN101926428B (en) | Probiotic gingko pollen freeze-dried powder and preparation method thereof | |
| CN102174450A (en) | Lactobacillus plantarum for resisting helicobacter pylori infection and application thereof | |
| CN109315769A (en) | It is a kind of for improving the composition and preparation method thereof of human body enteral environment | |
| CN103053738A (en) | Tea-processing herba cistanche and preparation method thereof | |
| CN110447799A (en) | A kind of complex solid plant beverage and preparation method thereof for improving intestinal flora | |
| CN107173656A (en) | A kind of Chinese medicine fruits and vegetables probiotics fermented beverage and preparation method thereof | |
| CN110447722A (en) | A kind of fermented glutinous rice yoghurt premixed powder and preparation method thereof | |
| CN104983022A (en) | Animal bifidobacterium fermented fruit-vegetable powder and preparing method and application thereof | |
| CN108936628A (en) | A kind of specific full nutritional formulas and preparation method thereof that patients with cerebral apoplexy is edible | |
| KR101394322B1 (en) | New Bacillus subtilis BCNU 9169 and probiotics composition comprising the same | |
| CN105802876B (en) | A kind of composite probiotics ferment clover tender shoots powder preparation and its preparation method and application | |
| CN104886569A (en) | An active probiotic fermented drink and a preparation method thereof | |
| CN105962375A (en) | Hericium erinaceus active lactobacillus preparation and preparation method thereof | |
| CN106360683A (en) | Dendrobium officinale and banana compound probiotic fermentation products and method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 101407 Beijing city Huairou District Yanqi Economic Development Zone mangniu River Road No. 31, No. 1 -2 Applicant after: Beijing branch of Hengtong biotechnology Limited by Share Ltd Address before: 101407 Beijing city Huairou District Yanqi Economic Development Zone mangniu River Road No. 31 hospital Applicant before: BEIJING KETUO HENGTONG BIOTECHNOLOGY DEVELOPMENT CO., LTD. |
|
| CB02 | Change of applicant information | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |