CN112546160A - Probiotic preparation for improving diabetes and preparation method thereof - Google Patents
Probiotic preparation for improving diabetes and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a probiotic preparation for improving diabetes, which comprises the following raw materials: butyric acid bacillus-fragrant solomonseal rhizome fermentation liquid, infant bifidobacteria-corn silk fermentation liquid, bacillus licheniformis, enterococcus faecium, lactobacillus casei, vitamins, prebiotics, dietary fiber, modified diatomite and modified taro powder, by adding the butyric acid bacillus-polygonatum fermentation liquor and the bifidobacterium infantis-corn silk fermentation liquor, the polygonatum extract is fermented by the butyric acid bacillus, so that the effective components in the polygonatum can be better extracted, meanwhile, the butyric acid bacillus is placed in the environment of the polygonatum extract, which is beneficial to the butyric acid bacillus to adapt to the drug effect environment of the polygonatum to generate synergistic action, the corn stigma has the functions of promoting urination and reducing blood pressure, can promote bile secretion, reduce blood viscosity and has obvious blood sugar reducing function, the bifidobacterium infantis is fermented, so that the drug effect is better extracted, the bifidobacterium infantis and the drug effect are complementary, the synergistic effect is realized, and the good improvement effect on the diabetes can be realized.
Description
Technical Field
The invention relates to the technical field of probiotic preparations, in particular to a probiotic preparation for improving diabetes and a preparation method thereof.
Background
High incidence of diabetes, multiple complications, high mortality and high disability rate. Diabetes is complicated with many diseases and complications, coronary heart disease, hypertension, myocardial infarction, cerebral apoplexy, senile dementia, Parkinson's disease, nephropathy, retinopathy and the like are complicated, and the incidence of cardiovascular and cerebrovascular complications is more than 3 times higher than that of non-diabetic people. Diabetic complications have become a fatal and disabling important disease species for diabetic patients.
Probiotics are generally considered to be active microorganisms that have a positive benefit to the host upon ingestion. Can effectively improve the structure of intestinal flora, inhibit pathogenic bacteria and produce good health effect.
Researches show that the composition of the intestinal flora varies among healthy people, obese people and type 2 diabetics, and the intestinal flora is a possible factor influencing the pathophysiology of metabolic diseases of organisms, so that the method for preventing or treating the metabolic diseases caused by high-fat diet through effectively regulating the intestinal flora is a method.
Disclosure of Invention
Embodiments of the present invention aim to provide a probiotic preparation for improving diabetes and a preparation method thereof, so as to solve the above problems.
In order to achieve the purpose, the invention provides the following technical scheme:
a probiotic preparation for improving diabetes comprises the following raw materials in parts by weight: 20-30 parts of butyric acid bacillus-polygonatum fermentation liquor, 15-25 parts of infant bifidobacteria-corn stigma fermentation liquor, 6-10 parts of bacillus licheniformis, 4-8 parts of enterococcus faecium, 5-9 parts of lactobacillus casei, 1-3 parts of vitamins, 8-12 parts of prebiotics, 10-20 parts of dietary fibers, 3-7 parts of modified diatomite and 4-8 parts of modified taro powder.
In one alternative: the feed comprises the following raw materials in parts by weight: 22-28 parts of butyric acid bacillus-polygonatum fermentation liquid, 17-23 parts of infant bifidobacteria-corn stigma fermentation liquid, 7-9 parts of bacillus licheniformis, 5-7 parts of enterococcus faecium, 6-8 parts of lactobacillus casei, 1.5-2.5 parts of vitamins, 9-11 parts of prebiotics, 13-17 parts of dietary fibers, 4-6 parts of modified diatomite and 5-7 parts of modified taro powder.
In one alternative: the feed comprises the following raw materials in parts by weight: 25 parts of butyric acid bacillus-polygonatum fermentation liquid, 20 parts of infant bifidobacterium-corn stigma fermentation liquid, 8 parts of bacillus licheniformis, 6 parts of enterococcus faecium, 7 parts of lactobacillus casei, 2 parts of vitamin, 10 parts of prebiotics, 15 parts of dietary fiber, 5 parts of modified diatomite and 6 parts of modified taro powder.
In one alternative: the preparation method of the butyric acid bacillus-polygonatum odoratum fermentation liquor comprises the following steps: activating butyric acid bacillus strain, and performing amplification culture to obtain viable bacteria with number of 1 × 107cfu/ml to 2X 107The method comprises the following steps of (1) dividing a butyric acid bacillus seed solution in a cfu/ml range into three parts by volume, inoculating one part by volume of the butyric acid bacillus seed solution into a fermentation tank filled with a mixed fermentation culture medium to obtain a primary culture solution, culturing the primary culture solution at 37 ℃ for 3-4 h, then inoculating two parts by volume of the butyric acid clostridium seed solution into the primary culture solution to obtain a secondary culture solution, adding a sterilized polygonatum extract into the secondary culture solution, and culturing the secondary culture solution at 37 ℃ for 22-26 h to finally obtain a butyric acid bacillus-polygonatum fermentation broth; the whole culture process is carried out under stirring, and the pH values of the primary culture solution and the secondary culture solution are controlled to 6.5 to 6.6 by adding a pH regulator.
In one alternative: the preparation method of the bifidobacterium infantis-corn stigma fermentation solution comprises the following steps: activating Bifidobacterium infantis, and performing amplification culture to obtain viable bacteria with number of 1 × 107cfu/ml to 2X 107Inoculating the bifidobacterium infantis seed liquid in the range of cfu/ml into a fermentation tank filled with a mixed fermentation medium, and culturing for 10-12 h at 37 ℃; then adding sterilized stigma Maydis extract into fermentationCulturing for 8-12 h at 37 ℃ in a tank to obtain bifidobacterium infantis-corn silk fermentation liquid; the whole culture process is carried out under stirring, and the pH values of the primary culture solution and the secondary culture solution are controlled to 6.5 to 6.6 by adding a pH regulator.
In one alternative: the mixed fermentation medium comprises the following raw materials in parts by weight: 10-20 parts of peptone, 8-12 parts of yeast extract, 9-13 parts of beef extract, 7-11 parts of dry powder of pig pancreas, 1-3 parts of sodium chloride, 1-2 parts of magnesium sulfate, 1-2 parts of sodium bicarbonate, 10-16 parts of glucose and 12-18 parts of starch, wherein the pig pancreas is added into a culture medium, has the effects of benefiting lung, relieving cough, strengthening spleen, stopping dysentery, promoting lactation and moistening dryness, is commonly used for treating flaccidity cough, lung distention, dyspnea, hemoptysis, spleen deficiency and diarrhea, milk obstruction, rhagadia manus and pedis, infertility and diabetes, provides nutrients and is beneficial to improving the diabetes.
In one alternative: the prebiotics is one or two of fructo-oligosaccharide, lactosucrose and galacto-oligosaccharide.
In one alternative: the vitamin is one or more of vitamin B1, vitamin D3, vitamin A, vitamin C, and vitamin E.
In one alternative: the dietary fiber is one or more of xylooligosaccharide, apple pectin and pomelo peel pectin.
In one alternative: the preparation method of the modified diatomite comprises the following steps: taking diatomite, adding the diatomite into water which is 3-5 times of the weight of the diatomite, uniformly mixing, adding a tin coupling agent which is 6-10% of the weight of the diatomite, adjusting the pH value to 5-6 by using acetic acid, then adding aucubin which is 1-5% of the weight of the diatomite, uniformly mixing, adjusting the temperature to 60-70 ℃, keeping the temperature for 1-3 hours, then adding stearic acid which is 4-6% of the weight of the diatomite, uniformly stirring, and drying at 100-120 ℃ until the weight is constant to obtain the modified diatomite.
In one alternative: the preparation method of the modified taro powder comprises the following steps: uniformly mixing taro powder and attapulgite according to a mass ratio of 80-90: 5-7, heating to 80-90 ℃ by adopting a water bath, preserving heat for 10-30 min, grinding the mixture of the taro powder and the attapulgite at a grinding temperature of 50-60 ℃, performing microwave treatment on the mixture for 15-25 s by adopting 350W after grinding for 10min, further performing grinding for 10-20 min, performing microwave treatment for 10-20 s by adopting 400W, adding the ground mixture into a stearic acid solution with a mass concentration of 5-6%, stirring at a rotating speed of 500r/min for 2-3 h at a temperature of 30-40 ℃, filtering, drying the filtered material at a temperature of 33-35 ℃, and cooling to room temperature to obtain the modified taro powder.
The preparation method of the probiotic preparation for improving diabetes comprises the following steps:
1) preparing raw materials according to a ratio;
2) sequentially placing butyric acid bacillus-polygonatum fermentation liquor, infant bifidobacteria-corn stigma fermentation liquor, bacillus licheniformis, enterococcus faecium, lactobacillus casei, vitamins, prebiotics and dietary fibers in an aseptic mixer, and uniformly mixing;
3) and then adding the modified diatomite and the modified taro powder, stirring and mixing uniformly, pre-freezing for 2-3 hours at the temperature of-20 to-30 ℃, and then quickly transferring into a freeze dryer for freeze drying for 20-24 hours to obtain the probiotic preparation for improving the diabetes.
Compared with the prior art, the embodiment of the invention has the following beneficial effects:
by adding the butyric acid bacillus-polygonatum fermentation liquor and the bifidobacterium infantis-corn stigma fermentation liquor, the butyric acid bacillus is generally present in soil, intestinal tracts of animals and human bodies, is gram-positive anaerobic bacillus, can tolerate gastric acid to enter the intestinal tracts, secretes butyric acid which is an important nutrient substance for regenerating and repairing intestinal mucosa, repairs damaged intestinal mucosa, eliminates inflammation and nourishes the intestinal tracts, has good curative effect on intestinal diseases caused by various reasons including intestinal tract bacterial infection, tumor chemotherapy, surgical operation and the like, the polygonatum has the functions of nourishing yin and moisturizing lung, promoting the production of body fluid and nourishing stomach, strengthening heart and reducing blood sugar, the polygonatum extract is fermented by the butyric acid bacillus, can better extract the drug effect components in the polygonatum, and meanwhile, the butyric acid bacillus is placed in the synergistic environment of the polygonatum extract to help the butyric acid bacillus to adapt to the drug effect environment of the polygonatum so, the corn stigma has the functions of promoting urination and reducing blood pressure, can promote bile secretion, reduce blood viscosity and obviously reduce blood sugar, the medicine effect is better extracted by fermenting the bifidobacterium infantis, the bifidobacterium infantis and the medicine effect complement each other and are synergistic, the good improvement effect on diabetes can be achieved, and the synergistic effect of bacillus licheniformis, enterococcus faecium and lactobacillus casei and the synergistic effect of the composite strain is further improved; by adding the modified diatomite and the modified taro powder, the adsorption performance is greatly improved, more strains can be adsorbed, the improvement effect on diabetes mellitus is improved due to the more strains, and the two strains form protection on the strains and contribute to the strain to play a role.
Detailed Description
The present invention will be described in detail with reference to the following examples, which are provided for illustrative purposes only and are not intended to limit the scope of the present invention. Any obvious modifications or variations can be made to the present invention without departing from the spirit or scope of the present invention.
Example 1
Firstly, preparing the following raw materials in parts by weight: 20 parts of butyric acid bacillus-polygonatum odoratum fermentation liquor, 15 parts of infant bifidobacterium-corn stigma fermentation liquor, 6 parts of bacillus licheniformis, 4 parts of enterococcus faecium, 5 parts of lactobacillus casei, 1 part of vitamin, 8 parts of prebiotics, 10 parts of dietary fiber, 3 parts of modified diatomite and 4 parts of modified taro powder, and then sequentially placing the butyric acid bacillus-polygonatum odoratum fermentation liquor, the infant bifidobacterium puerarin fermentation liquor, the bacillus licheniformis, the enterococcus faecium, the lactobacillus casei, the vitamin, the prebiotics and the dietary fiber into an aseptic mixer for uniform mixing; and finally, adding the modified diatomite and the modified taro powder, stirring and mixing uniformly, pre-freezing for 2 hours at the temperature of minus 20 ℃, and then quickly transferring into a freeze dryer for freeze drying for 20 hours to obtain the probiotic preparation for improving the diabetes.
The preparation method of the butyric acid bacillus-polygonatum odoratum fermentation liquor comprises the following steps: activating butyric acid bacillus strain, and performing amplification culture to obtain viable bacteria with number of 1 × 107cfu/ml to 2X 107The butyric acid bacillus seed liquid in the cfu/ml range is divided into three parts by volume, and one part by volumeInoculating part of the clostridium butyricum seed solution into a fermentation tank filled with a mixed fermentation culture medium to obtain a primary culture solution, culturing the primary culture solution at 37 ℃ for 3h, then inoculating upper and lower parts by volume of clostridium butyricum seed solution into the primary culture solution to obtain a secondary culture solution, adding a sterilized polygonatum extract into the secondary culture solution, and culturing the secondary culture solution at 37 ℃ for 22h to finally obtain a clostridium butyricum-polygonatum fermentation liquor; the whole culture process is carried out under stirring, and the pH values of the primary culture solution and the secondary culture solution are controlled to be 6.5 by adding a pH regulator.
The preparation method of the bifidobacterium infantis-corn stigma fermentation solution comprises the following steps: activating Bifidobacterium infantis, and performing amplification culture to obtain viable bacteria with number of 1 × 107cfu/ml to 2X 107Inoculating Bifidobacterium infantis seed solution in cfu/ml range into a fermentation tank containing mixed fermentation medium, and culturing at 37 deg.C for 10 hr; then adding the sterilized corn stigma extract into a fermentation tank, and culturing at 37 ℃ for 8h to obtain bifidobacterium infantis-corn stigma fermentation solution; the whole culture process is carried out under stirring, and the pH values of the primary culture solution and the secondary culture solution are controlled to be 6.5 by adding a pH regulator.
The mixed fermentation medium comprises the following raw materials in parts by weight: 10 parts of peptone, 8 parts of yeast extract, 9 parts of beef extract, 7 parts of dry powder of pig pancreas, 1 part of sodium chloride, 1 part of magnesium sulfate, 1 part of sodium bicarbonate, 10 parts of glucose and 12 parts of starch, wherein the pig pancreas has the effects of benefiting lung, relieving cough, tonifying spleen, stopping dysentery, promoting lactation and moistening dryness by adding the pig pancreas into a culture medium, is commonly used for treating consumptive lung disease, cough, lung distension, dyspnea, hemoptysis, spleen deficiency, diarrhea, breast obstruction, rhagadia manus and pedalis, infertility and diabetes, provides nutrients and is also helpful for improving the diabetes.
The prebiotics are fructo-oligosaccharides.
The vitamin is vitamin B1.
The dietary fiber is xylo-oligosaccharide.
The preparation method of the modified diatomite comprises the following steps: taking diatomite, adding the diatomite into water which is 3 times of the weight of the diatomite, uniformly mixing, adding a tin coupling agent which is 6 percent of the weight of the diatomite, adjusting the pH value to be 5 by using acetic acid, then adding aucubin which is 1 percent of the weight of the diatomite, uniformly mixing, adjusting the temperature to 60 ℃, keeping the temperature for 1 hour, then adding stearic acid which is 4 percent of the weight of the diatomite, uniformly stirring, and then drying at the temperature of 100 ℃ to constant weight to obtain the modified diatomite.
The preparation method of the modified taro powder comprises the following steps: uniformly mixing the taro powder and the attapulgite according to a mass ratio of 80:5, heating to 80 ℃ by adopting a water bath, preserving heat for 10min, grinding the mixture of the taro powder and the attapulgite, controlling the grinding temperature at 50 ℃, grinding for 10min, treating for 15s by adopting 350W microwave, continuing to grind for 10min, treating for 10s by adopting 400W microwave, adding the ground mixture into a stearic acid solution with the mass concentration of 5%, stirring for 2h at the temperature of 30 ℃ at the rotating speed of 500r/min, filtering, drying the filtered material at the temperature of 33 ℃, and cooling to room temperature to obtain the modified taro powder.
Example 2
Firstly, preparing the following raw materials in parts by weight: 22 parts of butyric acid bacillus-polygonatum odoratum fermentation liquor, 17 parts of infant bifidobacterium-corn stigma fermentation liquor, 7 parts of bacillus licheniformis, 5 parts of enterococcus faecium, 6 parts of lactobacillus casei, 1.5 parts of vitamin, 9 parts of prebiotics, 13 parts of dietary fiber, 4 parts of modified diatomite and 5 parts of modified taro powder, and then sequentially placing the butyric acid bacillus-polygonatum odoratum fermentation liquor, the infant bifidobacterium-corn stigma fermentation liquor, the bacillus licheniformis, the enterococcus faecium, the lactobacillus casei, the vitamin, the prebiotics and the dietary fiber into an aseptic mixer for uniform mixing; and finally, adding the modified diatomite and the modified taro powder, stirring and mixing uniformly, pre-freezing for 2 hours at the temperature of minus 22 ℃, and then quickly transferring into a freeze dryer for freeze drying for 21 hours to obtain the probiotic preparation for improving the diabetes.
The preparation method of the butyric acid bacillus-polygonatum odoratum fermentation liquor comprises the following steps: activating butyric acid bacillus strain, and performing amplification culture to obtain viable bacteria with number of 1 × 107cfu/ml to 2X 107A butyric acid bacillus seed liquid in the range of cfu/ml, dividing the butyric acid bacillus seed liquid into three parts by volume, and dividing one part by volumeInoculating the clostridium butyricum seed solution into a fermentation tank filled with a mixed fermentation culture medium to obtain a primary culture solution, culturing the primary culture solution at 37 ℃ for 3h, then inoculating upper and lower two parts by volume of clostridium butyricum seed solution into the primary culture solution to obtain a secondary culture solution, adding a sterilized polygonatum extract into the secondary culture solution, and culturing the secondary culture solution at 37 ℃ for 23h to finally obtain a clostridium butyricum-polygonatum fermentation liquor; the whole culture process is carried out under stirring, and the pH values of the primary culture solution and the secondary culture solution are controlled to be 6.5 by adding a pH regulator.
The preparation method of the bifidobacterium infantis-corn stigma fermentation solution comprises the following steps: activating Bifidobacterium infantis, and performing amplification culture to obtain viable bacteria with number of 1 × 107cfu/ml to 2X 107Inoculating Bifidobacterium infantis seed solution in cfu/ml range into a fermentation tank containing mixed fermentation medium, and culturing at 37 deg.C for 10 hr; then adding the sterilized corn stigma extract into a fermentation tank, and culturing at 37 ℃ for 9h to obtain bifidobacterium infantis-corn stigma fermentation solution; the whole culture process is carried out under stirring, and the pH values of the primary culture solution and the secondary culture solution are controlled to 6.5 to 6.6 by adding a pH regulator.
The mixed fermentation medium comprises the following raw materials in parts by weight: 12 parts of peptone, 9 parts of yeast extract, 10 parts of beef extract, 8 parts of dry powder of pig pancreas, 1 part of sodium chloride, 1 part of magnesium sulfate, 1 part of sodium bicarbonate, 11 parts of glucose and 13 parts of starch, wherein the pig pancreas has the effects of benefiting lung, relieving cough, tonifying spleen, stopping dysentery, promoting lactation and moistening dryness by adding the pig pancreas into a culture medium, is commonly used for treating consumptive lung disease, cough, lung distension, dyspnea, hemoptysis, spleen deficiency, diarrhea, milk obstruction, rhagadia manus and pedalis, infertility and diabetes, provides nutrients and is also helpful for improving the diabetes.
The prebiotics are lactosucrose.
The vitamin is vitamin D3.
The dietary fiber is apple pectin.
The preparation method of the modified diatomite comprises the following steps: taking diatomite, adding the diatomite into water which is 3 times of the weight of the diatomite, uniformly mixing, adding a tin coupling agent which is 7% of the weight of the diatomite, adjusting the pH value to be 5 by using acetic acid, then adding aucubin which is 2% of the weight of the diatomite, uniformly mixing, adjusting the temperature to 62 ℃, keeping the temperature for 1h, adding stearic acid which is 4% of the weight of the diatomite, uniformly stirring, and then drying at 105 ℃ to constant weight to obtain the modified diatomite.
The preparation method of the modified taro powder comprises the following steps: uniformly mixing the taro powder and the attapulgite according to a mass ratio of 82:5, heating to 82 ℃ by adopting a water bath, preserving heat for 15min, grinding the mixture of the taro powder and the attapulgite, controlling the grinding temperature at 52 ℃, grinding for 10min, treating for 17s by adopting 350W microwave, continuing to grind for 12min, treating for 12s by adopting 400W microwave, adding the ground mixture into a stearic acid solution with the mass concentration of 5%, stirring for 2h at the temperature of 32 ℃ at the rotating speed of 500r/min, filtering, drying the filtered material at the temperature of 33 ℃, and cooling to room temperature to obtain the modified taro powder.
Example 3
Firstly, preparing the following raw materials in parts by weight: 25 parts of butyric acid bacillus-polygonatum odoratum fermentation liquid, 20 parts of infant bifidobacterium-corn stigma fermentation liquid, 8 parts of bacillus licheniformis, 6 parts of enterococcus faecium, 7 parts of lactobacillus casei, 2 parts of vitamin, 10 parts of prebiotics, 15 parts of dietary fiber, 5 parts of modified diatomite and 6 parts of modified taro powder, and then sequentially placing the butyric acid bacillus-polygonatum odoratum fermentation liquid, the infant bifidobacterium-corn stigma fermentation liquid, the bacillus licheniformis, the enterococcus faecium, the lactobacillus casei, the vitamin, the prebiotics and the dietary fiber into an aseptic mixer for uniform mixing; and finally, adding the modified diatomite and the modified taro powder, stirring and mixing uniformly, pre-freezing for 2.5 hours at the temperature of minus 25 ℃, and then quickly transferring into a freeze dryer for freeze drying for 22 hours to obtain the probiotic preparation for improving the diabetes.
The preparation method of the butyric acid bacillus-polygonatum odoratum fermentation liquor comprises the following steps: activating butyric acid bacillus strain, and performing amplification culture to obtain viable bacteria with number of 1 × 107cfu/ml to 2X 107A butyric acid bacillus seed liquid in the range of cfu/ml, dividing the butyric acid bacillus seed liquid into three parts by volume, and dividing one part by volumeInoculating the butyric acid bacillus seed solution into a fermentation tank filled with a mixed fermentation culture medium to obtain a primary culture solution, culturing the primary culture solution at 37 ℃ for 3.5h, then inoculating an upper and a lower two volume parts of butyric acid clostridium seed solution into the primary culture solution to obtain a secondary culture solution, adding a sterilized polygonatum extract, and culturing the secondary culture solution at 37 ℃ for 24h to finally obtain a butyric acid bacillus-polygonatum fermentation liquor; the whole culture process is carried out under stirring, and the pH values of the primary culture solution and the secondary culture solution are controlled to be 6.5 by adding a pH regulator.
The preparation method of the bifidobacterium infantis-corn stigma fermentation solution comprises the following steps: activating Bifidobacterium infantis, and performing amplification culture to obtain viable bacteria with number of 1 × 107cfu/ml to 2X 107Inoculating Bifidobacterium infantis seed solution in cfu/ml range into a fermentation tank containing mixed fermentation medium, and culturing at 37 deg.C for 11 hr; then adding the sterilized corn stigma extract into a fermentation tank, and culturing at 37 ℃ for 10h to obtain bifidobacterium infantis-corn stigma fermentation solution; the whole culture process is carried out under stirring, and the pH values of the primary culture solution and the secondary culture solution are controlled to be 6.6 by adding a pH regulator.
The mixed fermentation medium comprises the following raw materials in parts by weight: 15 parts of peptone, 10 parts of yeast extract, 11 parts of beef extract, 9 parts of dry powder of pig pancreas, 2 parts of sodium chloride, 1.5 parts of magnesium sulfate, 1.5 parts of sodium bicarbonate, 13 parts of glucose and 15 parts of starch, and by adding the pig pancreas into a culture medium, the pig pancreas has the effects of benefiting lung, relieving cough, strengthening spleen, stopping diarrhea, promoting lactation and moistening dryness, is commonly used for treating consumptive lung disease, cough, lung distention, dyspnea, hemoptysis, spleen deficiency, diarrhea, galactosis, rhagadia manus and pedalis, infertility and diabetes, provides nutrients and is also helpful for improving the diabetes.
The prebiotics are galacto-oligosaccharides.
The vitamin is vitamin A.
The dietary fiber is pomelo peel pectin.
The preparation method of the modified diatomite comprises the following steps: taking diatomite, adding the diatomite into water with the weight 4 times of that of the diatomite, uniformly mixing, adding a tin coupling agent accounting for 8% of the weight of the diatomite, adjusting the pH value to 5.5 by using acetic acid, then adding aucubin accounting for 3% of the weight of the diatomite, uniformly mixing, adjusting the temperature to 65 ℃, keeping the temperature for 2 hours, adding stearic acid accounting for 5% of the weight of the diatomite, uniformly stirring, and then drying at 110 ℃ to constant weight to obtain the modified diatomite.
The preparation method of the modified taro powder comprises the following steps: uniformly mixing taro powder and attapulgite according to a mass ratio of 85:6, heating to 85 ℃ by adopting a water bath, preserving heat for 20min, then grinding the mixture of the taro powder and the attapulgite, controlling the grinding temperature to 55 ℃, grinding for 10min, then treating for 20s by adopting 350W microwave, further grinding for 15min, then treating for 15s by adopting 400W microwave, adding the ground mixture into a stearic acid solution with the mass concentration of 5.5%, stirring for 2.5h at the rotating speed of 500r/min at the temperature of 35 ℃, filtering, drying the filtered product at the temperature of 35 ℃, and cooling to room temperature to obtain the modified taro powder.
Example 4
Firstly, preparing the following raw materials in parts by weight: 28 parts of butyric acid bacillus-polygonatum odoratum fermentation liquor, 23 parts of infant bifidobacterium-corn stigma fermentation liquor, 9 parts of bacillus licheniformis, 7 parts of enterococcus faecium, 8 parts of lactobacillus casei, 2.5 parts of vitamin, 11 parts of prebiotics, 17 parts of dietary fiber, 6 parts of modified diatomite and 7 parts of modified taro powder, and then sequentially placing the butyric acid bacillus-polygonatum odoratum fermentation liquor, the infant bifidobacterium-corn stigma fermentation liquor, the bacillus licheniformis, the enterococcus faecium, the lactobacillus casei, the vitamin, the prebiotics and the dietary fiber into an aseptic mixer for uniform mixing; and finally, adding the modified diatomite and the modified taro powder, stirring and mixing uniformly, pre-freezing for 3 hours at the temperature of minus 28 ℃, and then quickly transferring into a freeze dryer for freeze drying for 23 hours to obtain the probiotic preparation for improving the diabetes.
The preparation method of the butyric acid bacillus-polygonatum odoratum fermentation liquor comprises the following steps: activating butyric acid bacillus strain, and performing amplification culture to obtain viable bacteria with number of 1 × 107cfu/ml to 2X 107The butyric acid bacillus seed liquid in the cfu/ml range is divided into three parts by volume,inoculating one volume part of the clostridium butyricum seed liquid into a fermentation tank filled with a mixed fermentation culture medium to obtain a primary culture liquid, culturing the primary culture liquid at 37 ℃ for 4 hours, then inoculating two volume parts of clostridium butyricum seed liquid into the primary culture liquid to obtain a secondary culture liquid, adding a sterilized polygonatum extract, and culturing the secondary culture liquid at 37 ℃ for 25 hours to finally obtain a clostridium butyricum-polygonatum fermentation liquid; the whole culture process is carried out under stirring, and the pH values of the primary culture solution and the secondary culture solution are controlled to be 6.6 by adding a pH regulator.
The preparation method of the bifidobacterium infantis-corn stigma fermentation solution comprises the following steps: activating Bifidobacterium infantis, and performing amplification culture to obtain viable bacteria with number of 1 × 107cfu/ml to 2X 107Inoculating Bifidobacterium infantis seed solution in cfu/ml range into a fermentation tank containing mixed fermentation medium, and culturing at 37 deg.C for 12 hr; then adding the sterilized corn stigma extract into a fermentation tank, and culturing at 37 ℃ for 11h to obtain bifidobacterium infantis-corn stigma fermentation solution; the whole culture process is carried out under stirring, and the pH values of the primary culture solution and the secondary culture solution are controlled to be 6.6 by adding a pH regulator.
The mixed fermentation medium comprises the following raw materials in parts by weight: the pig pancreas has the effects of benefiting lung, relieving cough, tonifying spleen, stopping dysentery, promoting lactation and moistening dryness by adding the pig pancreas into a culture medium, is commonly used for treating consumptive lung disease, cough, lung distension, dyspnea, hemoptysis, spleen deficiency, diarrhea, milk obstruction, rhagadia manus et pedis, infertility and diabetes, provides nutrients and is also beneficial to improving the diabetes while providing the nutrients.
The prebiotics are fructo-oligosaccharide and lactosucrose in equal proportion.
The vitamins are vitamin B1, vitamin D3, vitamin A, and vitamin C.
The dietary fiber is mixed by xylo-oligosaccharide, apple pectin and the like.
The preparation method of the modified diatomite comprises the following steps: taking diatomite, adding the diatomite into water which is 5 times of the weight of the diatomite, uniformly mixing, adding a tin coupling agent which is 9 percent of the weight of the diatomite, adjusting the pH value to be 6 by using acetic acid, then adding aucubin which is 4 percent of the weight of the diatomite, uniformly mixing, adjusting the temperature to 68 ℃, keeping the temperature for 3 hours, adding stearic acid which is 6 percent of the weight of the diatomite, uniformly stirring, and then drying at 115 ℃ to constant weight to obtain the modified diatomite.
The preparation method of the modified taro powder comprises the following steps: uniformly mixing the taro powder and the attapulgite according to a mass ratio of 88:7, heating to 88 ℃ by adopting a water bath, preserving heat for 25min, grinding the mixture of the taro powder and the attapulgite, controlling the grinding temperature at 58 ℃, grinding for 10min, treating for 23s by adopting 350W microwave, continuing to grind for 18min, treating for 18s by adopting 400W microwave, adding the ground mixture into a stearic acid solution with the mass concentration of 6%, stirring for 3h at the temperature of 38 ℃ at the rotating speed of 500r/min, filtering, drying the filtered substance at the temperature of 35 ℃, and cooling to room temperature to obtain the modified taro powder.
Example 5
Firstly, preparing the following raw materials in parts by weight: 20 parts of butyric acid bacillus-polygonatum odoratum fermentation liquor, 15 parts of infant bifidobacterium-corn stigma fermentation liquor, 6 parts of bacillus licheniformis, 4 parts of enterococcus faecium, 5 parts of lactobacillus casei, 1 part of vitamin, 8 parts of prebiotics, 10 parts of dietary fiber, 3 parts of modified diatomite and 4 parts of modified taro powder, and then sequentially placing the butyric acid bacillus-polygonatum odoratum fermentation liquor, the infant bifidobacterium puerarin fermentation liquor, the bacillus licheniformis, the enterococcus faecium, the lactobacillus casei, the vitamin, the prebiotics and the dietary fiber into an aseptic mixer for uniform mixing; and finally, adding the modified diatomite and the modified taro powder, stirring and mixing uniformly, pre-freezing for 2 hours at the temperature of minus 30 ℃, and then quickly transferring into a freeze dryer for freeze drying for 20 hours to obtain the probiotic preparation for improving the diabetes.
The preparation method of the butyric acid bacillus-polygonatum odoratum fermentation liquor comprises the following steps: activating butyric acid bacillus strain, and performing amplification culture to obtain viable bacteria with number of 1 × 107cfu/mlThe method comprises the following steps of (1) dividing a butyric acid bacillus seed solution into three parts by volume within the range of 2 x 107cfu/ml, inoculating one part by volume of the butyric acid bacillus seed solution into a fermentation tank filled with a mixed fermentation culture medium to obtain a primary culture solution, culturing the primary culture solution at 37 ℃ for 3 hours, then inoculating two parts by volume of the butyric acid clostridium seed solution into the primary culture solution to obtain a secondary culture solution, adding a sterilized polygonatum extract into the secondary culture solution, and culturing the secondary culture solution at 37 ℃ for 22 hours to finally obtain a butyric acid bacillus-polygonatum fermentation broth; the whole culture process is carried out under stirring, and the pH values of the primary culture solution and the secondary culture solution are controlled to be 6.6 by adding a pH regulator.
The preparation method of the bifidobacterium infantis-corn stigma fermentation solution comprises the following steps: activating Bifidobacterium infantis, and performing amplification culture to obtain viable bacteria with number of 1 × 107cfu/ml to 2X 107Inoculating Bifidobacterium infantis seed solution in cfu/ml range into a fermentation tank containing mixed fermentation medium, and culturing at 37 deg.C for 10 hr; then adding the sterilized corn stigma extract into a fermentation tank, and culturing at 37 ℃ for 8h to obtain bifidobacterium infantis-corn stigma fermentation solution; the whole culture process is carried out under stirring, and the pH values of the primary culture solution and the secondary culture solution are controlled to be 6.6 by adding a pH regulator.
The mixed fermentation medium comprises the following raw materials in parts by weight: 10 parts of peptone, 8 parts of yeast extract, 9 parts of beef extract, 7 parts of dry powder of pig pancreas, 1 part of sodium chloride, 1 part of magnesium sulfate, 1 part of sodium bicarbonate, 10 parts of glucose and 12 parts of starch, wherein the pig pancreas has the effects of benefiting lung, relieving cough, tonifying spleen, stopping dysentery, promoting lactation and moistening dryness by adding the pig pancreas into a culture medium, is commonly used for treating consumptive lung disease, cough, lung distension, dyspnea, hemoptysis, spleen deficiency, diarrhea, breast obstruction, rhagadia manus and pedalis, infertility and diabetes, provides nutrients and is also helpful for improving the diabetes.
The prebiotics are fructo-oligosaccharide, lactosucrose and galacto-oligosaccharide which are mixed according to the ratio of 1:2: 1.
The vitamins include vitamin B1, vitamin D3, vitamin A, vitamin C, and vitamin E.
The dietary fiber is a mixture of xylo-oligosaccharide, apple pectin and pomelo peel pectin according to a ratio of 1:1: 2.
The preparation method of the modified diatomite comprises the following steps: taking diatomite, adding the diatomite into water which is 3 times of the weight of the diatomite, uniformly mixing, adding a tin coupling agent which is 6 percent of the weight of the diatomite, adjusting the pH value to be 5 by using acetic acid, then adding aucubin which is 1 percent of the weight of the diatomite, uniformly mixing, adjusting the temperature to 60 ℃, keeping the temperature for 1 hour, then adding stearic acid which is 4 percent of the weight of the diatomite, uniformly stirring, and then drying at the temperature of 100 ℃ to constant weight to obtain the modified diatomite.
The preparation method of the modified taro powder comprises the following steps: uniformly mixing the taro powder and the attapulgite according to a mass ratio of 80:5, heating to 80 ℃ by adopting a water bath, preserving heat for 10min, grinding the mixture of the taro powder and the attapulgite, controlling the grinding temperature at 50 ℃, grinding for 10min, treating for 15s by adopting 350W microwave, continuing to grind for 10min, treating for 10s by adopting 400W microwave, adding the ground mixture into a stearic acid solution with the mass concentration of 5%, stirring for 2h at the temperature of 30 ℃ at the rotating speed of 500r/min, filtering, drying the filtered material at the temperature of 33 ℃, and cooling to room temperature to obtain the modified taro powder.
Comparative example 1
On the basis of the example 3, no butyric acid bacillus-polygonatum odoratum fermentation liquor is contained;
comparative example 2
Replacing the butyric acid bacillus-polygonatum odoratum fermentation liquid with the butyric acid bacillus and polygonatum odoratum extracting solution on the basis of the embodiment 3;
comparative example 3
On the basis of example 3, no bifidobacterium infantis-corn silk fermentation broth was contained;
comparative example 4
Replacing the bifidobacterium infantis-corn stigma fermentation solution with a bifidobacterium infantis and corn stigma extracting solution on the basis of the embodiment 3;
comparative example 5
On the basis of the example 3, the lactobacillus-polygonatum odoratum fermentation liquid and the bifidobacterium infantis-corn silk fermentation liquid are not contained;
comparative example 6
A commercial probiotic preparation.
Performance verification
Test animal
The KM male mouse has the weight of 17-23g, the animal laboratory is of a hundred thousand clean grade, the room temperature is 25 +/-2 ℃, and the humidity is 60-80%. Culturing in 12-hour light and 12-hour dark environment.
Test method
Establishment of diabetic mouse model
Mice after adaptive feeding for one week are fasted for 24h, and the tail vein is injected with physiological saline solution of alloxan (50mg kg)-1) And strengthening once after 72h by using the same dosage, after one week, fasting for 4-5h, taking blood from tail veins to measure blood sugar value, wherein the blood sugar concentration is more than or equal to 11.1mmol/L, and obtaining 120 diabetic mice.
Animal grouping and treatment thereof
Dividing 120 diabetic mice into ten groups of 10 mice randomly, wherein eleven groups are respectively given with the preparations of examples 1-5 and comparative examples 1-6, and the rest group is used as a blank control group to be given with physiological saline, and the administration is performed by intragastric administration according to the administration standard of 0.04g/kg by taking the body weight as a standard; and (5) the blank control group is continuously perfused with physiological saline with the same volume for 10d, and the weight and the blood sugar of the mice are recorded after 20 d. The specific results are as follows:
the results show that the probiotic preparation prepared by the invention has the effects of assisting in reducing blood sugar and improving the conditions of diabetes patients, and the effect of reducing blood sugar is further enhanced through the synergistic effect of the butyric acid bacillus-polygonatum fermentation liquid and the infant bifidobacterium-corn stigma fermentation liquid, so that the probiotic preparation has wide market prospect.
By adding the butyric acid bacillus-polygonatum fermentation liquor and the bifidobacterium infantis-corn stigma fermentation liquor, the butyric acid bacillus is generally present in soil, intestinal tracts of animals and human bodies, is gram-positive anaerobic bacillus, can tolerate gastric acid to enter the intestinal tracts, secretes butyric acid which is an important nutrient substance for regenerating and repairing intestinal mucosa, repairs damaged intestinal mucosa, eliminates inflammation and nourishes the intestinal tracts, has good curative effect on intestinal diseases caused by various reasons including intestinal tract bacterial infection, tumor chemotherapy, surgical operation and the like, the polygonatum has the functions of nourishing yin and moisturizing lung, promoting the production of body fluid and nourishing stomach, strengthening heart and reducing blood sugar, the polygonatum extract is fermented by the butyric acid bacillus, can better extract the drug effect components in the polygonatum, and meanwhile, the butyric acid bacillus is placed in the synergistic environment of the polygonatum extract to help the butyric acid bacillus to adapt to the drug effect environment of the polygonatum so, the corn stigma has the functions of promoting urination and reducing blood pressure, can promote bile secretion, reduce blood viscosity and obviously reduce blood sugar, the medicine effect is better extracted by fermenting the bifidobacterium infantis, the bifidobacterium infantis and the medicine effect complement each other and are synergistic, the good improvement effect on diabetes can be achieved, and the synergistic effect of bacillus licheniformis, enterococcus faecium and lactobacillus casei and the synergistic effect of the composite strain is further improved; by adding the modified diatomite and the modified taro powder, the adsorption performance is greatly improved, more strains can be adsorbed, the improvement effect on diabetes mellitus is improved due to the more strains, and the two strains form protection on the strains and contribute to the strain to play a role.
The above description is only for the specific embodiments of the present disclosure, but the scope of the present disclosure is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present disclosure, and all the changes or substitutions should be covered within the scope of the present disclosure. Therefore, the protection scope of the present disclosure shall be subject to the protection scope of the claims.
Claims (9)
1. The probiotic preparation for improving diabetes is characterized by comprising the following raw materials in parts by weight: 20-30 parts of butyric acid bacillus-polygonatum fermentation liquor, 15-25 parts of infant bifidobacteria-corn stigma fermentation liquor, 6-10 parts of bacillus licheniformis, 4-8 parts of enterococcus faecium, 5-9 parts of lactobacillus casei, 1-3 parts of vitamins, 8-12 parts of prebiotics, 10-20 parts of dietary fibers, 3-7 parts of modified diatomite and 4-8 parts of modified taro powder.
2. The probiotic preparation for improving diabetes according to claim 1, characterized by comprising the following raw materials in parts by weight: 22-28 parts of butyric acid bacillus-polygonatum fermentation liquid, 17-23 parts of infant bifidobacteria-corn stigma fermentation liquid, 7-9 parts of bacillus licheniformis, 5-7 parts of enterococcus faecium, 6-8 parts of lactobacillus casei, 1.5-2.5 parts of vitamins, 9-11 parts of prebiotics, 13-17 parts of dietary fibers, 4-6 parts of modified diatomite and 5-7 parts of modified taro powder.
3. The probiotic preparation for improving diabetes according to claim 2, characterized by comprising the following raw materials in parts by weight: 25 parts of butyric acid bacillus-polygonatum fermentation liquid, 20 parts of infant bifidobacterium-corn stigma fermentation liquid, 8 parts of bacillus licheniformis, 6 parts of enterococcus faecium, 7 parts of lactobacillus casei, 2 parts of vitamin, 10 parts of prebiotics, 15 parts of dietary fiber, 5 parts of modified diatomite and 6 parts of modified taro powder.
4. The probiotic preparation for improving diabetes according to claim 1, wherein the preparation method of the butyric acid bacillus-polygonatum odoratum fermentation liquid is as follows: activating butyric acid bacillus strain, and performing amplification culture to obtain viable bacteria with number of 1 × 107cfu/ml to 2X 107The method comprises the following steps of (1) dividing a butyric acid bacillus seed solution in a cfu/ml range into three parts by volume, inoculating one part by volume of the butyric acid bacillus seed solution into a fermentation tank filled with a mixed fermentation culture medium to obtain a primary culture solution, culturing the primary culture solution at 37 ℃ for 3-4 h, then inoculating two parts by volume of the butyric acid clostridium seed solution into the primary culture solution to obtain a secondary culture solution, adding a sterilized polygonatum extract into the secondary culture solution, and culturing the secondary culture solution at 37 ℃ for 22-26 h to finally obtain a butyric acid bacillus-polygonatum fermentation broth; the whole culture processAll under stirring.
5. The probiotic preparation for improving diabetes according to claim 1, wherein the preparation method of the bifidobacterium infantis-corn silk fermentation solution is as follows: activating Bifidobacterium infantis, and performing amplification culture to obtain viable bacteria with number of 1 × 107cfu/ml to 2X 107Inoculating the bifidobacterium infantis seed liquid in the range of cfu/ml into a fermentation tank filled with a mixed fermentation medium, and culturing for 10-12 h at 37 ℃; then adding the sterilized corn stigma extracting solution into a fermentation tank, and culturing for 8-12 h at 37 ℃ to obtain an infant bifidobacterium-corn stigma fermentation solution; the whole culture process is carried out under stirring, and the pH values of the primary culture solution and the secondary culture solution are controlled to 6.5 to 6.6 by adding a pH regulator.
6. The probiotic preparation for improving diabetes according to claim 1, wherein the prebiotics are any one or two of fructo-oligosaccharide, lactosucrose and galacto-oligosaccharide.
7. The probiotic preparation for improving diabetes according to claim 1, characterized in that the vitamin is any one or more of vitamin B1, vitamin D3, vitamin A, vitamin C, vitamin E.
8. The probiotic preparation for improving diabetes according to claim 1, characterized in that the dietary fiber is any one or more of xylooligosaccharide, apple pectin and pomelo peel pectin.
9. The method for preparing a probiotic preparation for improving diabetes according to any one of claims 1 to 8, characterized by comprising the following steps:
preparing raw materials according to a ratio;
sequentially placing butyric acid bacillus-polygonatum fermentation liquor, infant bifidobacteria-corn stigma fermentation liquor, bacillus licheniformis, enterococcus faecium, lactobacillus casei, vitamins, prebiotics and dietary fibers in an aseptic mixer, and uniformly mixing;
and then adding the modified diatomite and the modified taro powder, stirring and mixing uniformly, pre-freezing for 2-3 hours at the temperature of-20 to-30 ℃, and then quickly transferring into a freeze dryer for freeze drying for 20-24 hours to obtain the probiotic preparation for improving the diabetes.
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| CN104983746A (en) * | 2015-06-18 | 2015-10-21 | 合生元（广州）健康产品有限公司 | Complex probiotics and preparation method |
| CN105039217A (en) * | 2015-07-16 | 2015-11-11 | 山东凤凰生物有限公司 | Hypoglycemic probiotic preparation and its preparation method |
| CN108902517A (en) * | 2018-05-16 | 2018-11-30 | 浙江省中医药研究院 | A kind of probiotic composition and its application |
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