CN114176170B - Functional feed additive and preparation method and application thereof - Google Patents
Functional feed additive and preparation method and application thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of veterinary drug feed additives, and discloses a functional feed additive, a preparation method and application thereof. The functional feed additive is prepared from the following raw materials in parts by weight: 3-5 parts of hybrid broussonetia papyrifera stem and leaf fermentation product, 2-3 parts of corncob fermentation product, 1-2 parts of bletilla striata stem and leaf fermentation product, 2-4 parts of mulberry stem and leaf fermentation product, 2-3 parts of costustoot stem and leaf fermentation product and 1-2 parts of cuttlebone. The invention uses the by-products of Chinese medicine or grain production such as the stem and leaf of broussonetia papyrifera, corncob, bletilla striata stem and leaf and the stem and leaf of costustoot to treat diarrhea by using the above medicines together with the functions of relieving diarrhea with astringents, repairing gastric mucosa, supplementing energy and protein.
Description
Technical Field
The invention belongs to the technical field of veterinary drug feed additives, and relates to a functional feed additive, a preparation method and application thereof.
Background
The diarrhea and diarrhea problems of lambs or calves in weaning period are quite common in many farms, the diarrhea rate is high, the highest diarrhea rate can reach 65-85%, the death rate can reach 25-40%, and great economic loss is caused for the breeders of ruminants. Therefore, a feed or a medicine with comprehensive nutrition and high digestibility is designed for ruminants, so that the gastrointestinal flora of the ruminants is fully established.
The traditional Chinese medicine is rich in various nutritional ingredients, the bioactive substances are also very rich, and the traditional Chinese medicine has the dual effects of nutrition and medicine, can prevent diseases and treat symptoms, has the characteristics of naturalness, small toxic and side effects, difficulty in generating drug resistance, medicine residues and the like, and meets the time requirements of pollution-free animal husbandry and production of green animal products. After the medicinal parts are collected by artificial planting, the method has certain environmental and economic significance for fully developing and utilizing the non-medicinal parts.
The hybrid broussonetia papyrifera is a novel variety cultivated by adopting a modern aviation technology through space carrying breeding, hybrid breeding and other means through screening and experimental planting for nearly 10 years. Compared with the traditional paper mulberry, the hybrid paper mulberry feed has the advantages of quick nature, extremely strong adaptability and stress resistance, barren resistance, drought resistance, pollution resistance and disease and insect damage resistance, high yield and huge economic value, the paper mulberry leaves contain rich plant crude proteins, the leaves, stems and barks of the paper mulberry leaves are rich in natural flavonoid physiologically active substances, the protein content is up to 26.1 percent and is 8 percent higher than that of alfalfa meal, the total energy and digestion energy, the metabolic energy, the net energy and the digestible crude protein content of the paper mulberry are all quite high, and the contents of nutritional ingredients such as crude fat, calcium, amino acid, vitamins, carbohydrates, trace elements and the like are also very high.
Disclosure of Invention
The invention aims at providing a functional feed additive, which is prepared from Chinese medicaments or byproducts of grain production, namely, the stem and leaf of Broussonetia papyrifera, corncob, bletilla striata stem and leaf and the stem and leaf of elecampane, wherein the medicaments are used for relieving diarrhea by astringents, repairing gastric mucosa and supplementing energy and protein, so that the function of treating diarrhea is exerted.
The second purpose of the invention is to provide a preparation method of the functional feed additive, wherein the stem leaves of the hybrid broussonetia papyrifera, the corncob, the stem leaves of the bletilla striata, the stem leaves of the mulberry and the stem leaves of the elecampane are fermented by adopting different strains respectively or after being mixed, the feed additive obtained after the fermentation of the fermentation products can improve the digestibility of the feed and change the palatability of the feed.
The second purpose of the invention is to provide the application of the functional feed additive in preparing medicines or feeds for preventing or treating diarrhea of ruminants.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the invention provides a functional feed additive which is prepared from the following raw materials in parts by weight: 3-5 parts of hybrid broussonetia papyrifera stem and leaf fermentation product, 2-3 parts of corncob fermentation product, 1-2 parts of bletilla striata stem and leaf fermentation product, 2-4 parts of mulberry stem and leaf fermentation product, 2-3 parts of costustoot stem and leaf fermentation product and 1-2 parts of cuttlebone.
In one technical scheme, the functional feed additive is prepared from the following raw materials in parts by weight: 5 parts of a hybrid broussonetia papyrifera stem and leaf ferment, 3 parts of a corncob ferment, 1 part of a bletilla striata stem and leaf ferment, 3 parts of a mulberry stem and leaf ferment, 2 parts of an costustoot stem and leaf ferment and 2 parts of cuttlebone.
In one technical scheme, the control index of the additive is as follows: polysaccharide content not less than 9.3%, flavone content not less than 6.1%, crude protein content not less than 17.4%, crude fat content not less than 5.6%, soluble fiber not less than 16.9%, and soluble carbohydrate not less than 23.5%.
In one technical scheme, the hybrid broussonetia papyrifera stem and leaf ferment is prepared by fermenting hybrid broussonetia papyrifera stem and leaf through lactobacillus acidophilus and bifidobacterium; the corncob ferment is prepared by fermenting corncob with saccharomycetes and lactobacillus acidophilus; the mulberry stem and leaf ferment is prepared by fermenting mulberry stem and leaf through bifidobacterium and streptococcus thermophilus; the fermentation product of the stems and leaves of the bletilla striata and the fermentation product of the stems and leaves of the costus root are obtained by fermenting stems and leaves of the bletilla striata and stems and leaves of the costus root through the bacillus bulgaricus and the streptococcus lactis.
In one technical scheme, the hybrid broussonetia papyrifera stem and leaf ferment, the corncob ferment, the bletilla striata stem and leaf ferment, the mulberry stem and leaf ferment and the costustoot stem and leaf ferment are prepared by mixing and fermenting hybrid broussonetia papyrifera stem and leaf, corncob, bletilla striata stem and leaf, mulberry stem and leaf and costustoot stem and leaf through lactobacillus acidophilus, bifidobacterium, streptococcus thermophilus, saccharomycetes, bulgaricus and streptococcus lactis.
The invention also discloses a preparation method of the functional feed additive, which comprises the following steps:
1) Taking the stems and leaves of the hybrid broussonetia papyrifera, the corncob, the stems and leaves of the bletilla striata, the stems and leaves of the mulberry and the stems and leaves of the elecampane, and respectively crushing the stems and leaves of the hybrid broussonetia papyrifera, the corncob, the stems and leaves of the bletilla striata, the stems and leaves of the mulberry and the stems and leaves of the elecampane into particles with the particle size of 1 cm;
2) Fermenting the crushed stems and leaves of the hybrid broussonetia papyrifera by adopting lactobacillus acidophilus and bifidobacterium with the viable bacteria ratio of 1:1, fermenting corncobs by adopting saccharomycetes and lactobacillus acidophilus with the viable bacteria ratio of 1:1, fermenting the stems and leaves of the mulberry by adopting bifidobacterium and streptococcus thermophilus with the viable bacteria ratio of 1:1, and fermenting the stems and leaves of the bletilla and the costustoot by adopting Bulgaria and streptococcus lactis with the viable bacteria ratio of 1:1;
3) Pulverizing Os Sepiae to 200 mesh to obtain micropowder;
4) Mixing the fermented product of stems and leaves of hybrid Broussonetia papyrifera, the fermented product of corncob, the fermented product of stems and leaves of rhizoma Bletillae, the fermented product of stems and leaves of Morus alba, the fermented product of stems and leaves of radix aucklandiae and the micropowder of Os Sepiae.
The invention also discloses another preparation method of the functional feed additive, which comprises the following steps:
1) Taking the stems and leaves of the hybrid broussonetia papyrifera, the corncob, the stems and leaves of the bletilla striata, the stems and leaves of the mulberry and the stems and leaves of the elecampane, and respectively crushing the stems and leaves of the hybrid broussonetia papyrifera, the corncob, the stems and leaves of the bletilla striata, the stems and leaves of the mulberry and the stems and leaves of the elecampane into particles with the particle size of 1 cm;
2) Uniformly mixing crushed stems and leaves of hybrid broussonetia papyrifera, corncob, bletilla striata stems and leaves, mulberry stems and leaves and costustoot stems and leaves, and fermenting by lactobacillus acidophilus, bifidobacterium, streptococcus thermophilus, saccharomycetes, bulgaricus and streptococcus lactis with the viable bacteria ratio of 1:1:1:1:1;
3) Pulverizing Os Sepiae to 200 mesh to obtain micropowder;
4) Mixing the fermented product of stems and leaves of hybrid Broussonetia papyrifera, the fermented product of corncob, the fermented product of stems and leaves of rhizoma Bletillae, the fermented product of stems and leaves of Morus alba, the fermented product of stems and leaves of radix aucklandiae and the micropowder of Os Sepiae.
The invention also discloses application of the functional feed additive in preparing medicines or feed seeds for preventing or treating diarrhea of ruminants.
Compared with the prior art, the invention has the beneficial effects that:
(1) The preparation process of the invention adopts different strains to ferment the stem leaves of the hybrid broussonetia papyrifera, the corncob, the stem leaves of the bletilla striata, the stem leaves of the mulberry and the stem leaves of the costustoot after being respectively or mixed, and the feed additive obtained after the fermentation of the fermentation products can improve the digestibility of the feed, change the palatability of the feed and prepare the granules more convenient to use.
(2) The invention uses the by-products of Chinese medicine or grain production, namely the hybrid broussonetia papyrifera stem and leaf, the corncob, the bletilla striata stem and leaf and the costustoot stem and leaf, wherein the hybrid broussonetia papyrifera stem and leaf and the mulberry stem and leaf have higher protein and flavone content, the broussonetia papyrifera stem and leaf has the effects of clearing heat and detoxicating, dispelling wind and relieving itching, and the mulberry leaf has the effects of dispelling wind and heat, clearing lung and moistening dryness, suppressing hyperactive liver and improving eyesight, cooling blood and stopping bleeding, and the two are main medicines; the bletilla striata stem leaves astringe to stop bleeding, detumescence and promoting granulation, the elecampane has the effects of promoting qi circulation to stop pain, regulating the middle warmer and removing stagnation, and the stem leaves of the broussonetia papyrifera stem leaves are used for assisting in performing the functions of astringing to stop diarrhea, softening liver and harmonizing stomach after being fermented; cuttlebone has the effects of astringing, stopping bleeding, astringing essence, stopping leukorrhagia, relieving hyperacidity and relieving pain; the corncob has high sugar content, and has high bioactivity content after fermentation with plant stems and leaves, and has stronger effect of supplementing energy, and the medicine can be used for relieving diarrhea with astringents, repairing gastric mucosa, supplementing energy and protein, thereby playing a role in treating diarrhea.
(3) The feed additive is introduced into prevention and treatment of diarrhea of ruminants, is economical and environment-friendly, thereby greatly reducing production cost, has the effect of regulating gastrointestinal flora after fermentation of each component, thereby enhancing the antidiarrheal effect, and has good prevention and treatment effects through clinical experiments.
Detailed Description
The following examples are illustrative of the present invention and are not intended to limit the scope of the invention. The technical means used in the examples are conventional means well known to those skilled in the art unless otherwise indicated. The test methods in the following examples are conventional methods unless otherwise specified.
Lactobacillus acidophilus (Lactobacillus acidophilus) colony used in the examples below contained viable bacteria 10 8 CFU/g, bifidobacterium colony containing viable bacteria 10 8 CFU/g, yeast (Saccharomyces cerevisiae) colony contains viable bacteria 10 7 CFU/g, streptococcus thermophilus (Streptococcus thermophilus) colony contains viable bacteria 10 6 CFU/g, colony of Bulgaria bacillus (Lactobacillus bulgaricus) contains viable bacteria 10 7 CFU/g, streptococcus lactis (Streptococcus lactis (Lister) Lohnis Lohnis) colony contains viable bacteria 10 8 CFU/g。
Example 1
1) Taking 5Kg of stem and leaf of hybrid broussonetia papyrifera, 3Kg of corncob, 1Kg of bletilla striata stem and leaf, 3Kg of mulberry stem and leaf and 2Kg of costustoot stem and leaf, and respectively crushing into particles with the particle size of 1 cm.
2) Fermenting crushed stems and leaves of the hybrid broussonetia papyrifera with lactobacillus acidophilus and bifidobacterium with viable bacteria ratio of 1:1, wherein each 10 8 Adding 5Kg of hybrid broussonetia papyrifera stems and leaves into CFU/g strain, and fermenting at 25-37 ℃ for 20h; fermenting corncob with yeast and lactobacillus acidophilus at viable bacteria ratio of 1:1, wherein each 10 6 Adding 3Kg corncob into CFU/g strain, fermenting at 24-32deg.C for 12-24 hr; fermenting stems and leaves of mulberry with bifidobacterium and streptococcus thermophilus at a viable bacteria ratio of 1:1, wherein each 10 7 Adding 3Kg of mulberry stems and leaves into CFU/g unit strain, and fermenting at 30-32deg.C for 12-24 hr; fermenting the stems and leaves of bletilla striata and the stems and leaves of costus root by using Bulgaria bacillus and streptococcus lactis with the viable bacteria ratio of 1:1, wherein each 10 6 Adding 1Kg of bletilla tuber stem and leaf and 2Kg of radix aucklandiae stem and leaf into CFU/g strain, and fermenting at 30-35deg.C for 24-36 hr.
3) Pulverizing Os Sepiae to 200 mesh to obtain micropowder.
4) Taking 3kg of hybrid broussonetia papyrifera stem and leaf ferment, 2kg of corncob ferment, 1kg of bletilla striata stem and leaf ferment, 2kg of mulberry stem and leaf ferment, 2kg of costustoot stem and leaf ferment and 1kg of cuttlebone micropowder, and uniformly mixing to obtain the functional feed additive.
Example 2
This embodiment is substantially the same as embodiment 1 except that: fermenting the stem and leaf of the hybrid broussonetia papyrifera in the step 2 by adopting saccharomycetes and bifidobacteria with the viable bacteria ratio of 1:1, wherein each 10 8 Adding 5Kg of hybrid broussonetia papyrifera stems and leaves into CFU/g strain; the corncob is fermented by using bifidobacterium and lactobacillus acidophilus with viable bacteria ratio of 1:1, every 10 6 Adding 3Kg of corncob into CFU/g unit strain; fermenting the stems and leaves of the mulberry with Bulgaria bacillus and Streptococcus thermophilus with viable bacteria ratio of 1:1, wherein each 10 7 Adding 3Kg of mulberry stems and leaves into CFU/g strain; fermenting the stems and leaves of bletilla striata and the stems and leaves of elecampane by using bifidobacterium and streptococcus lactis with the viable bacteria ratio of 1:1, wherein each 10 8 CFU/g unit strain is added with 1Kg of bletilla striataStem and leaf and 2Kg of radix aucklandiae stem and leaf.
Example 3
This embodiment is substantially the same as embodiment 1 except that: fermenting the stem and leaf of the hybrid broussonetia papyrifera in the step 2 by using Bulgaria and bifidobacteria with the viable bacteria ratio of 1:1, wherein each 10 8 Adding 5Kg of hybrid broussonetia papyrifera stems and leaves into CFU/g strain; the corncob is fermented by using bifidobacterium and lactobacillus acidophilus with viable bacteria ratio of 1:1, every 10 7 Adding 3Kg of corncob into CFU/g strain; fermenting the stems and leaves of the mulberry with saccharomycetes and streptococcus thermophilus with the ratio of viable bacteria being 1:1, wherein each 10 8 Adding 3Kg of mulberry stems and leaves into CFU/g strain; fermenting the stems and leaves of bletilla striata and the stems and leaves of radix aucklandiae by using streptococcus thermophilus and streptococcus lactis with the viable bacteria ratio of 1:1, wherein each 10 7 1Kg of bletilla striata stem and leaf and 2Kg of radix aucklandiae stem and leaf are added to CFU/g of strain.
Example 4
This embodiment is substantially the same as embodiment 1 except that: fermenting the stem and leaf of the hybrid broussonetia papyrifera in the step 2 by adopting streptococcus thermophilus and bifidobacterium with the viable bacteria ratio of 1:1, wherein each 10 8 Adding 5Kg of hybrid broussonetia papyrifera stems and leaves into CFU/g unit strain; the corncob is fermented by streptococcus thermophilus and lactobacillus acidophilus with viable bacteria ratio of 1:1, every 10 7 Adding 3Kg of corncob into CFU/g unit strain; fermenting the stems and leaves of the mulberry with Bulgaria and Streptococcus thermophilus with viable bacteria ratio of 1:1, wherein each 10 6 Adding 3Kg of mulberry stems and leaves into CFU/g unit strain; fermenting the stems and leaves of bletilla striata and the stems and leaves of costustoot by adopting saccharomycetes and streptococcus lactis with the viable bacteria ratio of 1:1, wherein each 10 7 1Kg of bletilla striata stem and leaf and 2Kg of radix aucklandiae stem and leaf are added to CFU/g of unit strain.
The results of measuring polysaccharides, flavones, crude proteins, crude fats, soluble fibers and soluble carbohydrates in the fermented products obtained in step 2 of examples 1 to 4 and evaluating acidity and palatability are shown in table 2.
TABLE 2 detection results of each of the fermented products obtained in examples 4 to 6
The comparison shows that the ferment obtained in the example 1 has moderate acidity and good palatability; the acidity PH of each of the ferments obtained in examples 2-4 ranged from 3.8-6.7, but were less palatable and had lower levels of each nutrient than in example 1. Therefore, lactobacillus acidophilus and bifidobacterium are selected as the fermentation bacteria of the stem and leaf of the hybrid broussonetia papyrifera; the fermentation bacteria of the corncob are selected from saccharomycetes and lactobacillus acidophilus; the fermentation bacteria of the mulberry stems and leaves are selected from bifidobacteria and streptococcus thermophilus; the fermentation bacteria of the stems and leaves of bletilla striata and the stems and leaves of costustoot are selected from the group consisting of Bacillus bulgaricus and Streptococcus lactis. The bifidobacteria can decompose alpha-casein through phosphoprotein phosphatase to promote protein absorption, and lactobacillus acidophilus further ferments carbohydrate in the stem and leaf of the paper mulberry to produce lactic acid to promote protein digestion and absorption; the mulberry stem and leaf has higher protein and acid component content, the bifidobacterium can decompose alpha-casein through phosphoprotein phosphatase, promote protein absorption, and the streptococcus thermophilus has strong acid resistance, and L-lactic acid and folic acid are produced in the fermentation process; the corn cob has high sugar content, the saccharomycete is a facultative anaerobic microorganism, and can ferment and decompose sugar into alcohol and carbon dioxide, and lactobacillus acidophilus further ferments carbohydrate in the corn cob to generate lactic acid; the bletilla striata stem and leaf and the costustoot stem and leaf contain aromatic components, the lactic acid and acetic acid content can be increased in the fermentation process of streptococcus lactis, the bacillus bulgaricus can decompose saccharides to generate a large amount of lactic acid, and diacetyl and acetaldehyde are generated in the fermentation process to generate unique flavor.
Example 5
This embodiment is substantially the same as embodiment 1 except that: the ratio of viable bacteria in each used bacteria in the step 2 is 2:1.
Example 6
This embodiment is substantially the same as embodiment 1 except that: the ratio of viable bacteria in each used bacteria in the step 2 is 1:2.
The results of measuring polysaccharides, flavones, crude proteins, crude fats, soluble fibers and soluble carbohydrates in the fermented products obtained in step 2 of examples 5 to 6 and evaluating acidity and palatability are shown in table 1.
TABLE 1 detection results of each of the fermented products obtained in examples 1 to 3
The comparison shows that the ferment obtained in the example 5 has moderate acidity but poor palatability; the ferments obtained in example 6 were higher in acidity and less palatable than those obtained in example 1; and the content of each nutrient component is lower than that of the embodiment 1.
Example 7
1) Taking 5Kg of stem and leaf of hybrid broussonetia papyrifera, 3Kg of corncob, 1Kg of bletilla striata stem and leaf, 3Kg of mulberry stem and leaf and 2Kg of costustoot stem and leaf, and respectively crushing into particles with the particle size of 1 cm.
2) Mixing above crushed stems and leaves of Broussonetia papyrifera, corncob, rhizoma Bletillae, mulberry stems and leaves, and radix aucklandiae stems and leaves uniformly, fermenting with Lactobacillus acidophilus, bacillus bifidus, streptococcus thermophilus, yeast, bulgaria Bacillus and Streptococcus lactis at viable bacteria ratio of 1:1:1:1:1:1, wherein each 10 strains are used 8 10Kg of CFU/g is added, and fermentation is carried out for 12-24 hours at 25-35 ℃.
3) Pulverizing Os Sepiae to 200 mesh to obtain micropowder.
4) Taking 1kg of hybrid broussonetia papyrifera stem and leaf ferment, 1kg of corncob ferment, 1kg of bletilla striata stem and leaf ferment, 1kg of mulberry stem and leaf ferment, 1kg of costustoot stem and leaf ferment and 1kg of cuttlebone micropowder, and uniformly mixing to obtain the functional feed additive.
Examples 8 to 14
The present examples 8 to 14 are basically the same as example 7, except that: in the step 3, the mass fraction ratio of the fermentation product of the stems and leaves of the hybrid paper mulberry to the fermentation product of the corncob, the fermentation product of the stems and leaves of the bletilla striata to the fermentation product of the stems and leaves of the mulberry, the fermentation product of the stems and leaves of the costus root to the cuttlebone is respectively 2:2:1:2:2:2, 3:3:1:2:2:2, 4:3:1:2:2:2, 5:3:1:3:2:2, 6:3:1:3:2:2, 7:3:1:3:2:2 and 8:4:2:3:2:2 (based on 1kg of the fermentation product of the stems and leaves of the hybrid paper mulberry).
EXAMPLE 15 prescription Effect experiment
(1) Purpose of test
In the experiment, the functional feed additives of examples 7-14 are selected according to the modern breeding conditions and the growth rule of the lambs, the lambs are subjected to experimental screening, the weight gain, the fecal forming rate and the like of the lambs are used as indexes, the treatment condition of the traditional Chinese medicine formula on the diarrhea of the lambs is observed, and finally, the formula with an exact screening effect lays a foundation for later study.
(2) Test method
The weight of the diarrhea lambs 160 which appear in the weaning period (90+/-10 days old) is divided into 16 groups, and the formulas of the functional feed additives of the groups 1 to 8 are examples 7 to 14. Group 9 is 6 components with the ratio of 0:1:1:1:1:1, group 10 is 6 components with the ratio of 1:0:1:1:1, group 11 is 6 components with the ratio of 1:1:0:1:1:1, group 12 is 6 components with the ratio of 1:1:1:0:1:1, group 13 is 6 components with the ratio of 1:1:1:1:0:1, group 14 is 6 components with the ratio of 1:1:1:1:0, group 15 is antibiotic treatment group (gentamicin) and group 16 blank control group, each group dose is 0.5g/kg body weight, the disease condition of diarrhea is counted after 5 days of administration, whether drinking water, food, exercise and the like are normal or not is observed daily, and the rate and death rate of each group are comprehensively compared at the end of the test.
(3) Test results and analysis
Table 3 lamb diarrhea statistics for each group
As can be seen from table 3, the clinical effect of the functional feed additives of examples 7 to 14 on lamb diarrhea is better than that of the blank group, the lamb feces of examples 10 and 11 are formed higher than gentamicin, and the cure rate of example 11 is highest; as is known from comparative experiments (groups 9 to 14) lacking a certain component, the fermentation product of the stems and leaves of Broussonetia papyrifera and the fermentation product of the stems and leaves of bletilla striata are key components. So select the hybrid broussonetia papyrifera stem and leaf ferment: corn cob ferment: fermented product of stems and leaves of bletilla striata: mulberry stem and leaf fermented product: fermented product of stems and leaves of radix aucklandiae: the weight ratio of cuttlebone to cuttlebone is 5:3:1:3:2:2, and the cuttlebone is used as the optimal formula ratio of the functional feed additive.
EXAMPLE 16 pharmacodynamic Studies
(1) Test method
30 lambs with diarrhea in the weaning period (90+/-10 days old) are equally divided into 3 groups according to the weight, namely, a functional feed additive group, an antibiotic treatment group (gentamicin) and a control group, which are obtained in the embodiment 11, wherein the dosage of each group is 0.5g/kg of the weight, the treatment is observed for 10 days after 5 days of administration, the diarrhea incidence is counted, whether drinking water, feeding, exercise and the like are normal or not every day is recorded and observed, and the cure rate and the death rate of each group are comprehensively compared at the end of the test.
(2) Test results and analysis
Table 4 lamb diarrhea statistics for each group
Only 1 lamb dies in the test process in the control group, and the clinical effect of the functional feed additive in the treatment of the lamb diarrhea is obviously better than the treatment effect of antibiotics as shown in Table 4, the average weight and the forming number of feces of the lamb are higher than those of gentamicin and the control group, and the lamb fur of the functional feed additive group is bright and good in health state, and meanwhile, the functional feed additive provided by the invention can obviously improve the cure rate of the lamb diarrhea.
The above-mentioned embodiments are merely preferred embodiments of the present invention, which are not intended to limit the scope of the present invention, and other embodiments can be easily made by those skilled in the art through substitution or modification according to the technical disclosure in the present specification, so that all changes and modifications made in the principle of the present invention shall be included in the scope of the present invention.
Claims (5)
1. A functional feed additive for preventing or treating diarrhea of ruminants is characterized by being prepared from the following raw materials in parts by weight: 3-5 parts of hybrid broussonetia papyrifera stem and leaf fermentation product, 2-3 parts of corncob fermentation product, 1-2 parts of bletilla striata stem and leaf fermentation product, 2-4 parts of mulberry stem and leaf fermentation product, 2-3 parts of costustoot stem and leaf fermentation product and 1-2 parts of cuttlebone;
the hybrid broussonetia papyrifera stem and leaf ferment is prepared by fermenting hybrid broussonetia papyrifera stem and leaf with lactobacillus acidophilus and bifidobacterium in a viable bacteria proportion of 1:1; the corncob ferment is prepared by fermenting corncobs with viable bacteria ratio of 1:1 by saccharomycetes and lactobacillus acidophilus; the mulberry stem and leaf ferment is prepared by fermenting mulberry stem and leaf with bifidobacterium and streptococcus thermophilus in a viable bacteria proportion of 1:1; the fermentation product of the stems and leaves of the bletilla striata and the fermentation product of the stems and leaves of the costus root are obtained by fermenting the stems and leaves of the bletilla striata and the stems and leaves of the costus root by using Bulgarian bacillus and streptococcus lactis with the viable bacteria ratio of 1:1.
2. The functional feed additive for preventing or treating diarrhea in ruminants according to claim 1, which is characterized by being prepared from the following raw materials in parts by weight: 5 parts of a hybrid broussonetia papyrifera stem and leaf ferment, 3 parts of a corncob ferment, 1 part of a bletilla striata stem and leaf ferment, 3 parts of a mulberry stem and leaf ferment, 2 parts of an costustoot stem and leaf ferment and 2 parts of cuttlebone.
3. A functional feed additive for preventing or treating diarrhea in ruminants according to claim 1 or 2, characterized in that the control index of the additive is as follows: polysaccharide content is not less than 9.3%, flavone content is not less than 6.1%, crude protein content is not less than 17.4%, crude fat content is not less than 5.6%, soluble fiber content is not less than 16.9%, and soluble carbohydrate content is not less than 23.5%.
4. A method for preparing a functional feed additive for preventing or treating diarrhea in ruminants according to claim 1 or 2, characterized by comprising the steps of:
1) Taking the stems and leaves of the hybrid broussonetia papyrifera, the corncob, the stems and leaves of the bletilla striata, the stems and leaves of the mulberry and the stems and leaves of the elecampane, and respectively crushing the stems and leaves of the hybrid broussonetia papyrifera, the corncob, the stems and leaves of the bletilla striata, the stems and leaves of the mulberry and the stems and leaves of the elecampane into particles with the particle size of 1 cm;
2) Fermenting the crushed stems and leaves of the hybrid broussonetia papyrifera by adopting lactobacillus acidophilus and bifidobacterium with the viable bacteria ratio of 1:1, fermenting corncobs by adopting saccharomycetes and lactobacillus acidophilus with the viable bacteria ratio of 1:1, fermenting the stems and leaves of the mulberry by adopting bifidobacterium and streptococcus thermophilus with the viable bacteria ratio of 1:1, and fermenting the stems and leaves of the bletilla and the costustoot by adopting Bulgaria and streptococcus lactis with the viable bacteria ratio of 1:1;
3) Pulverizing Os Sepiae to 200 mesh to obtain micropowder;
4) Mixing the fermented product of stems and leaves of hybrid Broussonetia papyrifera, the fermented product of corncob, the fermented product of stems and leaves of rhizoma Bletillae, the fermented product of stems and leaves of Morus alba, the fermented product of stems and leaves of radix aucklandiae and the micropowder of Os Sepiae.
5. Use of a functional feed additive according to claim 1 or 2 in the preparation of a medicament or feed for preventing or treating diarrhea in ruminants.
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