CN113080335A

CN113080335A - Fermented traditional Chinese medicine additive for improving egg poultry production performance and preparation method thereof

Info

Publication number: CN113080335A
Application number: CN202110541643.7A
Authority: CN
Inventors: 张春杰; 董森; 孙书华; 李海利; 高航; 柴贝贝; 花朋朋
Original assignee: Zhengzhou Saike Pharmaceutical Technology Co ltd
Current assignee: Zhengzhou Saike Pharmaceutical Technology Co ltd
Priority date: 2021-05-18
Filing date: 2021-05-18
Publication date: 2021-07-09

Abstract

The invention discloses a fermented traditional Chinese medicine additive for improving the production performance of laying poultry and a preparation method thereof, belonging to the technical field of feed additives; the paint specifically comprises the following components in parts by weight: 5-15 parts of liquorice, 15-25 parts of dried orange peel, 25-35 parts of hawthorn, 25-35 parts of Chinese yam, 25-35 parts of divaricate saposhnikovia root, 55-65 parts of bighead atractylodes rhizome, 55-65 parts of astragalus membranaceus, 25-35 parts of epimedium herb and 55-65 parts of isatis root; adding liquorice, dried orange peel, hawthorn and Chinese yam into yeast and medicated leaven for aerobic liquid fermentation, then adding radix sileris, bighead atractylodes rhizome, astragalus, epimedium and isatis root for anaerobic liquid fermentation to obtain an intermediate fermentation product, adding lactobacillus plantarum, bacillus subtilis and bacillus licheniformis into the intermediate fermentation product, and then performing solid fermentation to obtain a traditional Chinese medicine additive; the invention improves the absorption and utilization rate of the traditional Chinese medicine components of the laying fowl, improves the medicinal effect, and solves the problems of the egg laying rate reduction caused by insufficient qi and blood of the laying fowl and abnormal oviduct.

Description

Fermented traditional Chinese medicine additive for improving egg poultry production performance and preparation method thereof

Technical Field

The invention belongs to the technical field of feed additives, and relates to a fermented traditional Chinese medicine additive prepared by biological fermentation engineering and used for improving the production performance of laying poultry and a preparation method thereof.

Background

The production of laying birds aims at laying eggs, but with the rapid development of the laying bird industry, many problems are exposed. First, the decrease in laying rate is a major factor affecting the performance of laying birds. Current egg production decreases generally include several situations: the eggs of the laying poultry which just start to lay are not laid; the peak rises slowly and has no peak; the laying rate is unstable or decreases. The clinical manifestations of instable or declining laying rate are generally that the symptoms of a large group are good, the laying rate suddenly declines by one to two or more, soft preserved eggs, blood spot eggs, sand preserved eggs and small eggs appear, and feces sometimes appear to be overfeeding, yellow-green feces and sometimes normal feces.

All symptoms expressed on eggshells are generally caused by the pathological changes of fallopian tubes, and due to the phenomena of dropsy, bleeding and the like of the fallopian tubes, eggs are discharged through the fallopian tubes, and the eggshells are polluted to generate blood spots, sand skin and the like. The whole egg laying system of the poultry consists of an ovary, an oviduct and a cloaca, wherein the ovary generates follicles, calcium is prepared at the horn mouth of the oviduct to form an eggshell, and the eggshell is discharged out of the body through the oviduct and the cloaca, which is the whole egg laying process, so that the abnormality of the eggshell, the preserved sand eggs, the soft preserved eggs, the blood spot eggs and the like can be generated when the oviduct is diseased.

The main factor determining the high and low laying rate is the quality of the ovarian function. When the ovary is normal, follicles can be secreted normally to form eggs, when the ovary is abnormal, no follicles are generated or few follicles are generated, no eggs are formed, and the laying rate is also obviously reduced. The reason for influencing ovarian follicle discharge is analyzed, firstly, organic damage to the ovary caused by some diseases in the early stage causes the ovary to develop abnormally and cannot discharge follicles normally, the condition is irreversible, and a elimination method is generally adopted to reduce loss. Secondly, considering feed and environmental factors, the protein energy of the feed is insufficient, so that the physique of the laying fowl cannot be kept up, the traditional Chinese medicine says that the qi and blood are insufficient, eggs are products of the qi and blood of the laying fowl, the qi and blood are insufficient, ovaries cannot normally discharge follicles, some environmental factors such as mould, stress and the like cause the digestive absorption capacity of a digestive system to be blocked, nutrient components in the feed cannot be normally converted, the nutrition cannot be kept up, ovaries cannot normally discharge follicles, the stress can cause partial functional disorder of an organism, the qi and blood are excessively consumed, and the ovaries cannot discharge follicles.

Moreover, the long-term egg laying of the laying fowl causes the qi and blood consumption of the laying fowl to be too large, and the ovary can not normally discharge follicles, namely, the egg laying fatigue disease is often said. In addition, poultry is restricted by a plurality of objective factors in the process of breeding, such as drinking water, the poultry usually drinks normal-temperature water, the temperature of the water in winter is very low, the poultry usually drinks, a lot of cold can be detained in vivo, the ovary is damaged by qi and blood, and follicles can not be secreted normally. In summer, ambient temperature is high, and the chicken does not have sweat gland under the chicken coop temperature to the feather is adhered to whole body, can only cool down through a large amount of drinking water, and the phenomenon of poultry diarrhea often can be found in this season, and excessive diarrhea also can cause the loss of qi and blood, and the ovary can not normally secrete the follicle.

In conclusion, deficiency of qi and blood is the root cause of the decrease in laying rate, and abnormality of the fallopian tube is the main cause of the abnormality of the eggshell. In the prior art, the immunity of laying fowls is improved by adding traditional Chinese medicines in the feed processing of the laying fowls, so that the disease incidence rate is reduced, but the feed is limited by factors such as the component influence and the addition amount of the feed, the effect of the laying fowls on absorbing and utilizing the traditional Chinese medicine components added in the feed is not good, and the problem of reduction of the laying rate still exists. At present, the traditional Chinese medicines are treated in a fermentation mode to improve the absorption and utilization rate of the traditional Chinese medicines, but because the traditional Chinese medicines are numerous and various and have complex components, the direct fermentation influences the metabolism of microorganisms, the fermentation and degradation are insufficient, and the absorption and utilization effect of the laying poultry in the feeding process is not ideal.

Disclosure of Invention

The invention overcomes the defects of the prior art, provides a fermented traditional Chinese medicine additive for improving the production performance of laying fowls and a preparation method thereof, aims to improve the absorption and utilization rate of traditional Chinese medicines, improve the medicinal effect and solve the problems of low laying rate caused by insufficient qi and blood of the laying fowls and abnormal fallopian tubes.

In order to achieve the above object, the present invention is achieved by the following technical solutions.

A fermented traditional Chinese medicine additive for improving the production performance of egg-laying poultry comprises the following components in parts by weight: 5-15 parts of liquorice, 15-25 parts of dried orange peel, 25-35 parts of hawthorn, 25-35 parts of Chinese yam, 25-35 parts of divaricate saposhnikovia root, 55-65 parts of bighead atractylodes rhizome, 55-65 parts of astragalus membranaceus, 25-35 parts of epimedium herb and 55-65 parts of isatis root.

Adding yeast and medicated leaven into liquorice, dried orange peel, hawthorn and Chinese yam for aerobic liquid fermentation, then adding radix sileris, bighead atractylodes rhizome, astragalus, epimedium and isatis root for anaerobic liquid fermentation to obtain an intermediate fermentation product, adding lactobacillus plantarum, bacillus subtilis and bacillus licheniformis into the intermediate fermentation product, and then performing solid fermentation to obtain the traditional Chinese medicine additive.

Preferably, corn meal is added in the aerobic liquid fermentation process for co-fermentation.

Preferably, solid-state fermentation auxiliary materials are added in the solid-state fermentation process.

Preferably, the yeast includes saccharomyces cerevisiae and aroma-producing yeast.

A preparation method of a fermented traditional Chinese medicine additive for improving the production performance of egg-laying poultry comprises the following steps:

a) mixing Glycyrrhrizae radix, pericarpium Citri Tangerinae, fructus crataegi, rhizoma Dioscoreae and semen Maydis powder, adding Massa Medicata Fermentata, yeast and water, and performing aerobic liquid fermentation to obtain fermentation product A.

b) Adding radix Saposhnikoviae, Atractylodis rhizoma, radix astragali, herba Epimedii, and radix Isatidis into the fermented product A, and performing anaerobic liquid state fermentation to obtain fermented product B.

c) Inoculating lactobacillus plantarum, bacillus subtilis and bacillus licheniformis into the fermentation product B, and adding a solid fermentation auxiliary material for solid fermentation.

Preferably, the temperature of the aerobic liquid state fermentation is 28-32 ℃, and the time is 48-72 hours; the anaerobic liquid state fermentation time is 48-72 hours; the solid state fermentation temperature is 38-42 ℃, and the time is 7-10 days.

Preferably, in the step a, the medicated leaven and the yeast are firstly added into warm water with the temperature of 35-40 ℃ for soaking for 1-2 hours, and then aerobic liquid state fermentation is carried out.

Preferably, the liquorice, the dried orange peel, the hawthorn, the Chinese yam, the divaricate saposhnikovia root, the largehead atractylodes rhizome, the astragalus root, the epimedium herb and the indigowoad root are crushed and sieved firstly.

Preferably, the solid-state fermentation auxiliary material comprises the following components in parts by weight: corn germ meal, namely bran, bean pulp = 4-6: 4-6: 1-3; the weight ratio of the fermentation product B to the solid fermentation auxiliary material is 1: 2-2.2.

Compared with the prior art, the invention has the following beneficial effects:

the product of the invention is designed and produced aiming at multiple factors of qi and blood deficiency, resistance reduction and laying rate reduction in the growth process of laying fowls, the fermented traditional Chinese medicine additive for improving the production performance of the laying fowls is designed and produced, the disease resistance of the laying fowls is fundamentally improved, and the problem of laying rate reduction caused by qi and blood deficiency of the laying fowls and abnormal fallopian tubes is solved. The traditional Chinese medicine additive has the following advantages:

(1) regulating intestinal flora balance, improving intestinal environment, reducing intestinal diseases, supplementing active enzyme and bioactive peptide, and improving growth and production performance of livestock and fowl.

(2) Improving immunity and disease resistance, effectively reducing virus diseases and bacterial diseases, and improving absorption rate and utilization rate of nutrient substances.

(3) Reducing the incidence of sudden death of the laying fowls, effectively reducing the incidence rate of common diseases of the digestive tracts of the laying fowls, and supplementing vitamins and trace elements.

(4) Promoting growth potential and production potential of egg-laying fowl, high efficiency, low toxicity, enhancing animal immunity, resisting stress, replenishing blood, invigorating qi, consolidating constitution, strengthening body resistance, and eliminating pathogenic factor.

(5) Inhibit, inactivate and degrade various moulds and mycotoxins, avoid the damage of livestock and poultry caused by mycotoxins, and invigorate qi and detoxify.

(6) The finished product of the invention has uniform color, and produces abundant organic acid, amino acid and prebiotics oligosaccharide through fermentation, thereby covering the bitter taste of the traditional Chinese medicine and improving the palatability.

(7) The traditional Chinese medicine composition can provide a growth environment for thalli, and can be decomposed and metabolized by the thalli to generate various effective active ingredients and nutrient substances, so that full-medicine property extraction is realized, and the medicinal efficacy is improved.

(8) The thallus used by the invention is active probiotics, can decompose macromolecular substances into easily absorbed micromolecules, can supplement the number of probiotics in bodies of laying fowls, and maintains the balance of the probiotics and the facultative pathogenic bacteria in the bodies.

According to the preparation method of the fermented traditional Chinese medicine additive, according to the growth and metabolism characteristics of different strains and the traditional Chinese medicine property mechanism, a fermentation process of front liquid and back solid is adopted, the fermentation process is regulated and controlled in fermentation mode, temperature and time according to the growth and metabolism characteristics of different microorganisms, the adding sequence of different traditional Chinese medicines is reasonably controlled, the traditional Chinese medicines are divided into two groups which respectively enter a liquid fermentation aerobic fermentation link and an anaerobic fermentation link, so that the two-way regulation and control of the decomposition and metabolism of the effective components of the traditional Chinese medicines and the growth and metabolism of the microorganisms are exerted, and the effects of the traditional Chinese medicines, the microorganisms and the metabolites of the microorganisms are superposed to the maximum extent.

In the aerobic fermentation stage of liquid fermentation, the main components of fermentation microorganisms are aspergillus niger, aspergillus oryzae, rhizopus, saccharomyces cerevisiae and aroma-producing yeast in medicated leaven, a large amount of amylase, glucose invertase, protease, lipase and the like are metabolized in the growth process of the aspergillus by activating and proliferating the microorganisms, polysaccharide rich in staple food corn flour, liquorice, dried orange peel, Chinese yam and hawthorn is gradually decomposed into reductive monosaccharide which can be directly utilized by the saccharomyces cerevisiae and the aroma-producing yeast, the reductive monosaccharide is further decomposed and metabolized into micromolecular alcohol, the alcohol is continuously enriched in the process, and the gradual metabolic characteristic of the elimination is formed between the aspergillus and the yeast.

The licorice and the dried orange peel are rich in polysaccharide substances and also contain a large amount of biological substances such as liquiritin, glycyrrhizic acid, dried orange peel glycoside and the like, and the substances are easier to dissolve in a solution containing alcohol under the catabolism of microorganisms. Meanwhile, as the alcoholic strength in a fermentation system gradually rises, the growth and metabolism of mould and yeast which play a role in the early stage are inhibited, the fungus and yeast tend to die down to form mycoprotein, and organic components in the liquorice, the dried orange peel, the Chinese yam and the hawthorn which contain a large amount of macromolecular polysaccharide are degraded, so that the subsequent interference on the full dissolution of the functional components is reduced, and the digestibility of the egg-laying poultry is improved.

Adding Chinese medicinal components with specific effects such as radix Saposhnikoviae, Atractylodis rhizoma, radix astragali, herba Epimedii, and radix Isatidis in liquid fermentation closed fermentation stage, wherein the Chinese medicinal components mainly comprise linarin in radix Saposhnikoviae, atractylone in Atractylodis rhizoma, astragaloside IV in radix astragali, icariin in herba Epimedii, and (R, S) -goitrin in radix Isatidis; since the liquorice, the dried orange peel, the Chinese yam, the hawthorn and the corn flour are fermented in advance, the environment for effectively dissolving out the specific functional components is provided, so that the functional components are enriched and completed at the stage, and the subsequent solid state fermentation is facilitated.

In the solid state fermentation system, the main components of the fermenting microorganisms are lactobacillus plantarum, bacillus subtilis and bacillus licheniformis, anaerobic fermentation is realized due to the solid state fermentation, and the moisture content is relatively low. In the fermentation process, the microorganisms further convert alcohol, attenuated mycoprotein, bran, soybean meal and other nutrients produced in the liquid fermentation process into small molecule metabolites such as organic acid, amino acid, fatty acid, glycerin and the like, so that the flavor of the fermented traditional Chinese medicine is improved, and the digestion, absorption and metabolism of animals are promoted in later-stage application.

Detailed Description

In order to make the technical problems, technical solutions and advantageous effects to be solved by the present invention more clearly apparent, the present invention is further described in detail with reference to the embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention. The technical solutions of the present invention are described in detail below with reference to examples, but the scope of protection is not limited thereto.

Example 1

A fermented traditional Chinese medicine additive for improving the production performance of egg-laying poultry comprises the following components in parts by weight: 10 parts of liquorice, 20 parts of dried orange peel, 30 parts of hawthorn, 30 parts of Chinese yam, 30 parts of divaricate saposhnikovia root, 60 parts of largehead atractylodes rhizome, 60 parts of astragalus, 30 parts of epimedium and 60 parts of isatis root.

The preparation method comprises the following steps:

the method comprises the following steps: weighing the liquorice, the dried orange peel, the hawthorn and the Chinese yam according to the proportion, mixing, then crushing into 80 meshes, and uniformly mixing the crushed traditional Chinese medicine powder and 80 meshes of corn flour according to a ratio of 1:1 to obtain the staple food. The form of the medicinal powder is selected to improve the full fermentation of the traditional Chinese medicine raw materials and the nutritional raw materials in the liquid fermentation process, and avoid incomplete soaking and insufficient fermentation caused by overlarge granularity of the traditional Chinese medicine materials in the extraction process.

Step two: and (3) weighing medicated leaven according to 10% of the total weight of the main grain in the step one, adding saccharomyces cerevisiae and aroma-producing yeast respectively according to 2% of the total weight of the main grain, uniformly mixing the medicated leaven and the aroma-producing yeast, adding warm water at 35-40 ℃ for soaking for 1-2 hours to serve as liquid fermentation bacteria liquid, and the soaking is to ensure that microorganisms and aroma-producing yeast in the medicated leaven are fully activated.

Step three: adding warm water of 30-35 ℃ which is 3-4 times of the staple food into the staple food in the first step, pouring the liquid fermentation bacterium liquid in the second step into the staple food fermentation liquid, and uniformly stirring to ensure that the staple food, the bacterium liquid and the warm water are fully and uniformly mixed; and ensuring that the temperature of the fermentation liquor is controlled between 28 and 32 ℃, and the fermentation is carried out for 48 to 72 hours in an open fermentation way, so as to promote the rapid growth, proliferation and metabolism of microorganisms.

Step four: weighing the divaricate saposhnikovia root, the largehead atractylodes rhizome, the astragalus root, the epimedium herb and the isatis root according to the proportion, mixing, crushing to 30 meshes, adding into main grain fermentation liquor, carrying out closed fermentation for 48-72 h, and converting macromolecular substances in the traditional Chinese medicinal materials into micromolecular substances through the growth and metabolism of microorganisms; after the closed fermentation is finished, lactobacillus plantarum, bacillus subtilis and bacillus licheniformis are respectively added into the main grain fermentation liquor according to 1 per mill of the total weight of the main grain, and then the mixture is subjected to closed incubation for 1-2 hours to obtain liquid fermentation liquor.

Step five: and (3) mixing the liquid fermentation liquor fermented in the fourth step with fermentation nutrients (corn germ meal: bran: soybean meal = 5: 5: 2) in proportion, and entering a solid fermentation stage. Wherein the ratio of the fermentation liquor to the fermentation nutrient is controlled to be 1: 2-2.2, so as to ensure that the water content of the solid fermentation material is 30-35%. And (3) putting the fermentation material into a fermentation bag with a breather valve, and keeping the fermentation temperature at 38-42 ℃ for solid-state fermentation for 7-10 days.

The fermented traditional Chinese medicine additive is designed by 10 traditional Chinese medicines of liquorice, dried orange peel, red dates, hawthorn, Chinese yam, radix sileris, bighead atractylodes rhizome, astragalus, epimedium and radix isatidis, takes corn flour, corn germ meal and soybean meal as main nutrient sources, takes Saccharomyces cerevisiae (the deposition number is ATCC9763, the Latin name is Saccharomyces cerevisiae), lipid-producing and aroma-producing saccharomycete (the deposition number is CICC NO. 32235), lactobacillus plantarum (the deposition number is CICC NO. 21794), bacillus subtilis (the deposition number is CICC NO. 10066) for producing amylase and bacillus (the deposition number is CICC NO. 10037) for producing protease as main fermentation strains, takes medicated leaven as auxiliary leaven, and is prepared by a pre-liquid post-solid fermentation process to convert macromolecular substances in the nutrient sources into micromolecular substances, dissolve main functional components in the traditional Chinese medicines step by step to play a quadruple effect superposition effect of traditional Chinese medicine functional components, probiotics, prebiotics and enzyme preparations, improve the production performance of the laying fowl.

Example 2

A fermented traditional Chinese medicine additive for improving the production performance of egg-laying poultry comprises the following components in parts by weight: 15 parts of liquorice, 25 parts of dried orange peel, 30 parts of hawthorn, 25 parts of Chinese yam, 25 parts of divaricate saposhnikovia root, 65 parts of largehead atractylodes rhizome, 55 parts of astragalus, 35 parts of epimedium herb and 65 parts of indigowoad root.

The preparation steps are different from the embodiment 1 in that liquorice, dried orange peel, hawthorn, Chinese yam and corn flour are crushed to 60 meshes; radix Saposhnikoviae, Atractylodis rhizoma, radix astragali, herba Epimedii, and radix Isatidis by mixing, pulverizing to 20 mesh, and the rest steps are the same.

Example 3

A fermented traditional Chinese medicine additive for improving the production performance of egg-laying poultry comprises the following components in parts by weight: 5 parts of liquorice, 15 parts of dried orange peel, 35 parts of hawthorn, 35 parts of Chinese yam, 35 parts of divaricate saposhnikovia root, 55 parts of largehead atractylodes rhizome, 65 parts of astragalus, 25 parts of epimedium and 55 parts of isatis root.

The preparation procedure was the same as in example 1.

In examples 1-3, the nutrient feed: 45-55 parts of corn flour, 45-55 parts of corn germ meal, 15-25 parts of bean pulp and 45-55 parts of bran. Provides basic nutrient substances for microbial fermentation.

A leavening agent: medicated leaven, saccharomyces cerevisiae, aroma-producing yeast, lactobacillus plantarum, bacillus subtilis and bacillus licheniformis. Medicated leaven is prepared by mixing wheat flour, bran, almond paste, red bean powder, fresh sweet wormwood, fresh cocklebur, and fresh red-knees herb juice, stirring, making into small blocks, placing into a basket, covering with hemp leaf or broussonetia papyrifera leaf, fermenting for one week under heat preservation, growing hypha (fresh yellow coat), taking out, and drying in the sun. Unprocessed or stir-baked to a slightly scorched smell. Massa Medicata Fermentata is sweet, pungent and warm in nature. Enter spleen and stomach meridians, promote digestion, resolve food stagnation, invigorate stomach and regulate the middle warmer.

The monarch drug of the invention is astragalus. Huang Qi is sweet in flavor and slightly warm in nature. It enters lung, spleen, liver and kidney meridians. Has the effects of invigorating qi, consolidating exterior, arresting sweating, promoting granulation, inducing diuresis, and relieving swelling. Can be used for treating deficiency of vital energy, asthenia, collapse of middle-warmer energy, chronic diarrhea, proctoptosis, hematochezia, metrorrhagia, exterior deficiency, spontaneous perspiration, carbuncle, cellulitis, intractable ulcer, and blood deficiency. It is combined with Bai Zhu and shan Yao to tonify middle-jiao and Qi.

The astragalus root, the bighead atractylodes rhizome and the divaricate saposhnikovia root are main components of the classical famous prescription jade screen powder, are tonifying agents and have the effects of tonifying qi, strengthening exterior and suppressing sweating. Can be used for treating asthenia, exterior weakness, exterior qi weakness, and affection of exogenous wind-cold. In the formula, astragalus is sweet and warm, can tonify qi of spleen and lung internally, and can strengthen superficies to check sweating externally, and is a monarch drug; the bighead atractylodes rhizome, rhizoma atractylodis macrocephalae, as a ministerial drug, strengthens the functions of tonifying qi and strengthening exterior and assists the astragalus to strengthen the qi-tonifying and exterior-consolidating functions; the composition is used for assisting in preventing wind from going out of the body surface to dissipate wind evil, and is combined with the astragalus and the bighead atractylodes rhizome to tonify qi and eliminate evil. Moreover, the astragalus root can prevent the wind and strengthen the exterior without causing pathogenic factors; ledebouriella root, radix astragali, with the effect of eliminating pathogens without damaging the body resistance, has the effect of reinforcing the middle-jiao while relaxing, and reinforcing the middle-jiao while dispersing.

The radix astragali, the epimedium herb and the radix isatidis are main compositions of the body resistance strengthening and detoxifying powder, the main function of the body resistance strengthening and detoxifying powder is to achieve the effect of protecting and protecting the exterior by tonifying the body resistance of an animal body through the radix astragali, and the radix isatidis in the formula is bitter and cold in nature, enters liver and stomach channels, has the effects of clearing heat, detoxifying, cooling blood, relieving sore throat and increasing the stress resistance of the body to external adverse factors; the epimedium in the prescription can greatly tonify the true yang in the kidney, thereby further helping the astragalus root to improve the qi tonifying capability of the organism, and the three supplement each other.

Licorice root, radix Glycyrrhizae is sweet and neutral in nature. It enters heart, lung, spleen and stomach meridians. Has effects in invigorating spleen, invigorating qi, eliminating phlegm, relieving cough, regulating the middle warmer, relieving spasm, removing toxic substance, harmonizing the drugs, and relieving drug toxicity.

The tangerine peel has three main functions, namely guiding cold evil in chest, breaking stagnant qi and benefiting spleen and stomach. Of the three major actions, the main action is to move qi of spleen and stomach. The spleen and stomach are mainly used for transporting and transforming water-dampness, so qi of spleen and stomach can remove dampness, invigorate spleen and resolve phlegm, so it can be said that Chen Pi can warm and nourish spleen, pungent can activate spleen and bitter can invigorate spleen. Because Chen Pi governs qi movement of spleen and stomach, spleen and stomach are in the middle energizer and qi of middle energizer passes through, qi of triple energizer also surges.

Haw is sour and sweet in nature and slightly warm. It enters spleen, stomach and liver meridians. It is used for relieving dyspepsia and dispelling blood stasis. It is indicated for meat accumulation, abdominal mass, phlegm-fluid retention, fullness and distension, acid regurgitation, dysentery and intestinal wind. Promote digestion, invigorate stomach, promote qi circulation and dissipate blood stasis.

Yam is sweet, warm and mild, and has no toxicity. It is mainly used for reinforcing deficiency and win-win, removing cold and heat evil, reinforcing middle warmer, benefiting strength, growing muscle and strengthening yin. Tonify the five consumptive diseases and seven injuries, remove cold wind, calm the heart, tranquilize mind, tonify heart qi deficiency and open up the heart hole for recording many events. Replenishing kidney qi, strengthening spleen and stomach, stopping diarrhea, resolving phlegm and moistening skin and hair.

The liquorice, the dried orange peel, the hawthorn and the Chinese yam are medicinal and edible traditional Chinese medicinal materials, are rich in liquorice polysaccharide, dried orange peel polysaccharide, hawthorn polysaccharide and Chinese yam polysaccharide, are metabolized to generate various small molecular organic acids and the like in the fermentation process, and have good effects of adjusting the balance of intestinal flora of organisms, improving the environment in the intestinal tract and reducing the occurrence of intestinal diseases.

Test example 1

The fermented traditional Chinese medicine feed additive prepared in the embodiment 1 is detected by Henan Haizhen detection technology Limited (third party detection), and the main technical indexes are as follows:

test example 2

In order to verify the application effect of the fermentation type traditional Chinese medicine additive for improving the production performance of the laying fowl, the fermentation traditional Chinese medicine additive is applied to a certain laying hen farm in the New countryside in Henan. The experimental protocol and results were verified as follows:

and (3) experimental design: 50-week-old (350-day-old) Hailan laying hens are randomly divided into a control group, a test 1 group, a test 2 group and a test 3 group, wherein each group is 12000, and each group is divided into 10 repeated groups.

Feeding basal diet for the control group, wherein the feed is prepared according to the nutrition requirement of laying hens in NY/T33-2004 in China;

experiment 1 group in the first 15 days of basic daily ration, 5kg/t of the traditional Chinese medicine powder disclosed by the patent method is added, and the difference is that the traditional Chinese medicine is directly crushed to 30-40 meshes without microbial fermentation.

Experiment 2 groups added 5kg/t of liquid fermentation liquor prepared by the patented method to basic ration in the first 15 days, except that all traditional Chinese medicinal materials are put into the liquid fermentation link, only liquid fermentation is carried out, and solid fermentation nutrients are not added.

Experiment 3 groups of solid fermentation traditional Chinese medicine additives prepared by the patent method are added into basic daily ration of the previous 15 days for 5 kg/t.

The indexes of the chicken house such as the quantity of the stocked chicken house, the laying rate, the egg laying number, the death and culling rate and the like are recorded for 30 days before the test.

Feeding management: the experiment is carried out in 9-27-2020-11-2 days in 2020, a certain large-scale laying hen farm in Xinxiang of Henan, the pre-test period is 15 days, the experiment period is 30 days, 6 chickens are fed in each cage, the chickens are fed with food and water freely, the temperature of a henhouse is automatically controlled, the temperature is stabilized at 22-27 ℃, and feces are automatically cleaned and automatically illuminated every day. The method is carried out according to conventional feeding management, disinfection, immunization procedures and the like.

Index measurement: the laying rate, egg breaking rate, egg weight, feed intake, death and culling number and the like are recorded daily during the test period, and the average laying rate, average egg breaking rate, average egg weight, average feed intake, average feed-egg ratio and average death and culling rate of each treatment group are counted.

Data statistics processing: experimental data were analyzed for variance using One-way ANOVA from SPSS22.0, combined with Duncan's method for multiple comparisons, with P <0.05 indicating significant difference, P >0.05 indicating insignificant difference, and data expressed as mean ± standard deviation.

Note: data in the same column are annotated with the same lower case letters to indicate insignificant difference (P > 0.05) and annotated with different lower case letters to indicate significant difference (P < 0.05).

And (3) test results: from the above data analysis results, it can be seen that the test group 3 (solid fermentation traditional Chinese medicine feed additive) has significant differences (P < 0.05) in laying rate, egg breakage rate, death and culling rate, feed-egg ratio and death and culling rate compared with the control group. There were significant differences in laying rate, egg weight, feed-to-egg ratio and mortality as compared to trial 1 and trial 2 groups. Compared with the control group, the test 1 group and the test 2 group have significant differences in laying rate, egg breaking rate, egg weight and death and culling rate. The experiment 3 group has the most obvious effect on improving the laying rate, reducing the egg breaking rate, improving the average egg weight and reducing the feed-egg ratio and the death and culling rate. From the analysis results, the difference between the feed intake of the test group 3 and the feed intake of the test group 2 is not great, and the feed intake of the test group 1 is affected by directly adding the unfermented traditional Chinese medicine powder.

While the invention has been described in further detail with reference to specific preferred embodiments thereof, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

Claims

1. A fermented traditional Chinese medicine additive for improving the production performance of egg-laying poultry is characterized by comprising the following components in parts by weight: 5-15 parts of liquorice, 15-25 parts of dried orange peel, 25-35 parts of hawthorn, 25-35 parts of Chinese yam, 25-35 parts of divaricate saposhnikovia root, 55-65 parts of bighead atractylodes rhizome, 55-65 parts of astragalus membranaceus, 25-35 parts of epimedium herb and 55-65 parts of isatis root;

adding yeast and medicated leaven into liquorice, dried orange peel, hawthorn and Chinese yam to perform aerobic liquid fermentation, then adding radix sileris, bighead atractylodes rhizome, astragalus, epimedium and isatis root to perform anaerobic liquid fermentation to obtain an intermediate fermentation product, adding lactobacillus plantarum, bacillus subtilis and bacillus licheniformis into the intermediate fermentation product, and performing solid fermentation to obtain the traditional Chinese medicine additive.

2. The fermented traditional Chinese medicine additive for improving the laying fowl production performance according to claim 1, wherein corn flour is added in the aerobic liquid fermentation process for co-fermentation.

3. The fermented traditional Chinese medicine additive for improving the laying fowl production performance according to claim 1, wherein a solid state fermentation auxiliary material is added in the solid state fermentation process.

4. The fermented traditional Chinese medicine additive for improving the laying fowl production performance according to any one of claims 1 to 3, wherein the yeast comprises Saccharomyces cerevisiae and aroma-producing yeast.

5. A preparation method of a fermented traditional Chinese medicine additive for improving the production performance of egg-laying poultry is characterized by comprising the following steps:

a) mixing Glycyrrhrizae radix, pericarpium Citri Tangerinae, fructus crataegi, rhizoma Dioscoreae and semen Maydis powder, adding Massa Medicata Fermentata, yeast and water, and performing aerobic liquid fermentation to obtain fermented product A;

b) adding radix Saposhnikoviae, Atractylodis rhizoma, radix astragali, herba Epimedii, and radix Isatidis into the fermented product A, and performing anaerobic liquid state fermentation to obtain fermented product B;

6. The preparation method of the fermented traditional Chinese medicine additive for improving the production performance of the laying fowl according to claim 5, wherein the temperature of the aerobic liquid fermentation is 28-32 ℃ and the time is 48-72 h; the anaerobic liquid state fermentation time is 48-72 hours; the solid state fermentation temperature is 38-42 ℃, and the time is 7-10 days.

7. The preparation method of the fermented traditional Chinese medicine additive for improving the laying fowl production performance as claimed in claim 5, wherein in the step a, the medicated leaven and the yeast are firstly added into warm water with the temperature of 35-40 ℃ for soaking for 1-2 h, and then aerobic liquid state fermentation is carried out.

8. The method for preparing a fermented traditional Chinese medicine additive for improving egg-laying fowl production performance according to claim 5, wherein the licorice, the dried orange peel, the hawthorn, the yam, the divaricate saposhnikovia root, the largehead atractylodes rhizome, the astragalus, the epimedium herb and the radix isatidis are all subjected to crushing and sieving treatment.

9. The method for preparing fermented traditional Chinese medicine additive for improving laying fowl production performance according to claim 5, wherein the yeast comprises Saccharomyces cerevisiae and aroma-producing yeast.

10. The preparation method of the fermented traditional Chinese medicine additive for improving the laying fowl production performance according to claim 5, wherein the solid state fermentation auxiliary material comprises the following components in parts by weight: corn germ meal, namely bran, bean pulp = 4-6: 4-6: 1-3; the weight ratio of the fermentation product B to the solid fermentation auxiliary material is 1: 2-2.2.