CN114176170A - Functional feed additive and preparation method and application thereof - Google Patents

Functional feed additive and preparation method and application thereof Download PDF

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CN114176170A
CN114176170A CN202111397126.3A CN202111397126A CN114176170A CN 114176170 A CN114176170 A CN 114176170A CN 202111397126 A CN202111397126 A CN 202111397126A CN 114176170 A CN114176170 A CN 114176170A
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stem
leaf
fermented product
mulberry
parts
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CN114176170B (en
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马霞
郭振环
张志强
马刚
王雪飞
李向辉
韩开顺
朱富丽
刘永录
畅希彦
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Shandong Tiange Agriculture And Animal Husbandry Development Co ltd
Sheqi Jingou Agriculture And Animal Husbandry Co ltd
Henan Kangxing Pharmaceutical Co Ltd
Henan University of Animal Husbandry and Economy
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Shandong Tiange Agriculture And Animal Husbandry Development Co ltd
Sheqi Jingou Agriculture And Animal Husbandry Co ltd
Henan Kangxing Pharmaceutical Co Ltd
Henan University of Animal Husbandry and Economy
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    • A61K36/18—Magnoliophyta (angiosperms)
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    • A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
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    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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Abstract

The invention belongs to the technical field of traditional Chinese veterinary medicine feed additives, and discloses a functional feed additive and a preparation method and application thereof. The functional feed additive is prepared from the following raw materials in parts by weight: 3-5 parts of a broussonetia papyrifera stem and leaf fermented product, 2-3 parts of a corncob fermented product, 1-2 parts of a bletilla striata stem and leaf fermented product, 2-4 parts of a mulberry stem and leaf fermented product, 2-3 parts of an elecampane stem and leaf fermented product and 1-2 parts of cuttlebone. The invention combines the traditional Chinese medicine or the byproducts of grain production, namely the stem and leaf of the broussonetia papyrifera, the corncob, the stem and leaf of the bletilla striata and the stem and leaf of the elecampane with the effects of relieving diarrhea with astringents, repairing gastric mucosa and supplementing energy and protein, thereby playing the role of treating diarrhea.

Description

Functional feed additive and preparation method and application thereof
Technical Field
The invention belongs to the technical field of traditional Chinese veterinary medicine feed additives, and relates to a functional feed additive and a preparation method and application thereof.
Background
The diarrhea and diarrhea problem of the lamb or the calf in the weaning period is quite common in many farms, the diarrhea rate is high, the highest rate can reach 65-85%, the death rate can reach 25-40%, and the economic loss is caused for the breeders of the ruminants. Therefore, a feed or a drug with complete nutrition and high digestion and absorption rate is designed for the ruminant to ensure that the gastrointestinal flora is fully established.
The traditional Chinese medicine is rich in various nutritional ingredients, has very rich bioactive substances, has double effects of nutrition and medicines, can prevent diseases and treat diseases, has the characteristics of nature, small toxic and side effects, difficulty in generating drug resistance and drug residues and the like, and meets the requirements of times of pollution-free animal husbandry and production of green animal products. After the medicinal parts are artificially planted and collected, the method has certain environmental and economic significance for fully developing and utilizing the non-medicinal parts.
The hybrid paper mulberry is a novel variety cultivated by adopting the modern aviation technology and adopting means such as space carrying breeding, hybrid breeding and the like through screening and experimental planting for nearly 10 years. Compared with the traditional broussonetia papyrifera, the feed hybrid broussonetia papyrifera variety has quick growth, strong adaptability and stress resistance, barren resistance, drought resistance, pollution resistance and plant diseases and insect pests resistance, high yield and huge economic value, the broussonetia papyrifera leaves contain rich plant crude protein, the leaves, stems and barks of the broussonetia papyrifera are rich in natural flavonoid physiological active substances, the protein content is up to 26.1 percent and is 8 percent higher than that of alfalfa meal, the total energy, the digestion energy, the metabolic energy, the net energy and the digestible crude protein content are all quite high, and the contents of nutrient components such as crude fat, calcium, amino acid, vitamin, carbohydrate, trace elements and the like are also very high, so that the feed is a novel woody plant feed resource newly bred in recent years, can provide higher protein and energy, comprehensively improve the immune system level of livestock and poultry, and reduce the death rate.
Disclosure of Invention
One of the purposes of the invention is to provide a functional feed additive, which combines the Chinese medicines or byproducts of grain production, namely the stem and leaf of the broussonetia papyrifera, the corncob, the stem and leaf of the bletilla striata and the stem and leaf of the elecampane, with the effects of relieving diarrhea with astringents, repairing gastric mucosa and supplementing energy and protein, thereby playing the role of treating diarrhea.
The other purpose of the invention is to provide a preparation method of the functional feed additive, which is characterized in that the stem leaves of the broussonetia papyrifera, the corn cob, the stem leaves of the bletilla striata, the stem leaves of the mulberry and the stem leaves of the elecampane are fermented by different strains respectively or after being mixed, and the feed additive obtained after the fermentation products are mixed can improve the digestibility of the feed and change the palatability of the feed.
The other purpose of the invention is to provide the application of the functional feed additive in preparing a medicament or feed for preventing or treating ruminant diarrhea.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention provides a functional feed additive which is prepared from the following raw materials in parts by weight: 3-5 parts of a broussonetia papyrifera stem and leaf fermented product, 2-3 parts of a corncob fermented product, 1-2 parts of a bletilla striata stem and leaf fermented product, 2-4 parts of a mulberry stem and leaf fermented product, 2-3 parts of a aucklandia japonica stem and leaf fermented product and 1-2 parts of cuttlebone.
In one technical scheme, the functional feed additive is prepared from the following raw materials in parts by weight: 5 parts of fermentation product of stem and leaf of broussonetia papyrifera, 3 parts of fermentation product of corncob, 1 part of fermentation product of stem and leaf of bletilla striata, 3 parts of fermentation product of stem and leaf of mulberry, 2 parts of fermentation product of stem and leaf of elecampane and 2 parts of cuttlebone.
In one embodiment, the control criteria for the additive are as follows: the polysaccharide content is not less than 9.3%, the flavone content is not less than 6.1%, the crude protein content is not less than 17.4%, the crude fat content is not less than 5.6%, the soluble fiber content is not less than 16.9%, and the soluble carbohydrate content is not less than 23.5%.
In one technical scheme, the broussonetia papyrifera stem and leaf leavening is prepared by fermenting broussonetia papyrifera stem and leaf with lactobacillus acidophilus and bifidobacterium; the corn cob ferment is prepared by fermenting corn cobs with saccharomycetes and lactobacillus acidophilus; the mulberry stem and leaf fermented product is prepared by fermenting mulberry stem and leaf with bifidobacterium and streptococcus thermophilus; the bletilla striata stem and leaf fermented product and the costus root stem and leaf fermented product are prepared by fermenting bletilla striata stem and leaf and costus root stem and leaf with bacillus bulgaricus and streptococcus lactici.
In one technical scheme, the broussonetia papyrifera stem and leaf leavening, the corncob leavening, the bletilla striata stem and leaf leavening, the mulberry stem and leaf leavening and the elecampane stem and leaf leavening are prepared by mixing and fermenting broussonetia papyrifera stem and leaf, corncob, bletilla striata stem and leaf, mulberry stem and leaf and elecampane stem and leaf with lactobacillus acidophilus, bifidobacterium, streptococcus thermophilus, saccharomycetes, bulgaria bacillus and streptococcus lactis.
The invention also discloses a preparation method of the functional feed additive, which comprises the following steps:
1) respectively pulverizing stem and leaf of Broussonetia papyrifera, corn cob, stem and leaf of rhizoma Bletillae, stem and leaf of mulberry, and stem and leaf of radix aucklandiae to particles with particle size of 1 cm;
2) fermenting the crushed stem and leaf of the hybrid paper mulberry by using lactobacillus acidophilus and lactobacillus bifidus with the viable bacteria ratio of 1:1, fermenting the corn core by using saccharomycetes and lactobacillus acidophilus with the viable bacteria ratio of 1:1, fermenting the stem and leaf of the mulberry by using bifidobacterium bifidum and streptococcus thermophilus with the viable bacteria ratio of 1:1, and fermenting the stem and leaf of bletilla striata and the stem and leaf of elecampane by using bulgaria bacillus and streptococcus lactis with the viable bacteria ratio of 1: 1;
3) pulverizing Os Sepiae to 200 mesh to obtain Os Sepiae micropowder;
4) mixing the stem and leaf fermented product of Broussonetia papyrifera, the corn cob fermented product, the stem and leaf fermented product of rhizoma Bletillae, the stem and leaf fermented product of mulberry, the stem and leaf fermented product of radix aucklandiae and Os Sepiae micropowder.
The invention also discloses another preparation method of the functional feed additive, which comprises the following steps:
1) respectively pulverizing stem and leaf of Broussonetia papyrifera, corn cob, stem and leaf of rhizoma Bletillae, stem and leaf of mulberry, and stem and leaf of radix aucklandiae to particles with particle size of 1 cm;
2) uniformly mixing crushed stem and leaf of the hybrid paper mulberry, corncob, stem and leaf of bletilla striata, stem and leaf of mulberry and stem and leaf of costus root, and fermenting by lactobacillus acidophilus, bifidobacterium, streptococcus thermophilus, saccharomycete, lactobacillus bulgaricus and streptococcus lactis with viable bacteria ratio of 1:1:1:1: 1;
3) pulverizing Os Sepiae to 200 mesh to obtain Os Sepiae micropowder;
4) mixing the stem and leaf fermented product of Broussonetia papyrifera, the corn cob fermented product, the stem and leaf fermented product of rhizoma Bletillae, the stem and leaf fermented product of mulberry, the stem and leaf fermented product of radix aucklandiae and Os Sepiae micropowder.
The invention also discloses application of the functional feed additive in preparing a medicament or a feed for preventing or treating ruminant diarrhea.
Compared with the prior art, the invention has the beneficial effects that:
(1) in the preparation process, the stem and leaf of the broussonetia papyrifera, the corncob, the stem and leaf of the bletilla striata, the stem and leaf of the mulberry and the stem and leaf of the costus root are fermented by different strains respectively or after being mixed, and the feed additive obtained after the fermentates are mixed can improve the digestibility of the feed, change the palatability of the feed and be prepared into particles which are more convenient to use.
(2) The invention relates to a traditional Chinese medicine or grain production by-product hybrid paper mulberry stem and leaf, corncob, bletilla striata stem and leaf and costus root stem and leaf, wherein the hybrid paper mulberry stem and leaf and mulberry stem and leaf have higher protein and flavone content, paper mulberry bark has the effects of clearing heat, detoxifying, dispelling wind and relieving itching, mulberry leaf has the effects of dispelling wind and heat from the body, clearing lung, moistening dryness, calming the liver, improving eyesight, cooling blood and stopping bleeding, and the two are used as main medicines; bletilla striata stem and leaf astringes to stop bleeding, reduces swelling and promotes granulation, elecampane has the effects of promoting qi circulation, relieving pain, regulating middle warmer and removing food stagnation, and broussonetia papyrifera stem and leaf are assisted after fermentation to play the effects of astringing to stop diarrhea, softening liver and harmonizing stomach; cuttlebone has effects of astringing to stop bleeding, arresting seminal emission, stopping leukorrhagia, relieving hyperacidity and relieving pain; the corncob has high sugar content, and has high bioactivity content after being fermented with plant stems and leaves, and has strong energy supplementing effect.
(3) The feed additive is introduced into the prevention and treatment of the diarrhea of the ruminant, so that the feed additive is environment-friendly, the production cost is greatly reduced, the components have the function of regulating the gastrointestinal flora after fermentation, the curative effect of diarrhea is enhanced, and the additive has good prevention and treatment effects through clinical tests.
Detailed Description
The following examples are intended to illustrate the invention, but are not intended to limit the scope of the invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art. The test methods in the following examples are conventional methods unless otherwise specified.
The Lactobacillus acidophilus (Lactobacillus acidophilus) used in the following examples contains viable bacteria 10 in the colony8CFU/g, live bacterium 10 in Bifidobacterium (Bifidobacterium) colonies8CFU/g, viable bacteria 10 contained in yeast (Saccharomyces cerevisiae) colonies7CFU/g, viable bacteria 10 contained in colonies of Streptococcus thermophilus (Streptococcus thermophilus)6CFU/g, viable bacteria 10 contained in the colony of Bacillus bulgaricus (Lactobacillus bulgaricus)7CFU/g, viable bacteria 10 in Streptococcus lactis (Lister) Lohnis8CFU/g。
Example 1
1) 5Kg of stem and leaf of the broussonetia papyrifera, 3Kg of corncob, 1Kg of stem and leaf of bletilla striata, 3Kg of stem and leaf of mulberry and 2Kg of stem and leaf of costus root are taken and respectively crushed into particles with the particle size of 1 cm.
2) Fermenting pulverized stem and leaf of hybrid paper mulberry with Lactobacillus acidophilus and Bifidobacterium at viable ratio of 1:1, every 108Adding 5Kg of stem and leaf of hybrid paper mulberry into CFU/g of strain, and fermenting at 25-37 deg.C for 20 hr; fermenting corn cob with yeast and Lactobacillus acidophilus at viable ratio of 1:1, every 106Adding 3Kg of corncob into the strain CFU/g, and fermenting at 24-32 ℃ for 12-24 h; fermenting stem and leaf of mulberry with Bacillus bifidus and Streptococcus thermophilus at ratio of 1:1, every 107Adding 3Kg of mulberry stems and leaves into CFU/g unit strain, and fermenting at 30-32 deg.C for 12-24 hr; the stem and leaf of rhizoma Bletillae and the stem and leaf of radix aucklandiae are fermented by Bacillus bulgaricus and Streptococcus lactis at viable bacteria ratio of 1:1, 106Adding 1Kg of bletilla striata stem and leaf and 2Kg of costus root stem and leaf into CFU/g of strain, and fermenting at 30-35 deg.C for 24-36 h.
3) Pulverizing Os Sepiae to 200 mesh to obtain Os Sepiae micropowder.
4) The functional feed additive is prepared by uniformly mixing 3kg of fermentation product of stem and leaf of hybrid paper mulberry, 2kg of fermentation product of corncob, 1kg of fermentation product of stem and leaf of bletilla striata, 2kg of fermentation product of stem and leaf of mulberry, 2kg of fermentation product of stem and leaf of costus root and 1kg of micro powder of cuttlebone.
Example 2
This embodiment is substantially the same as embodiment 1 except that: in step 2, living bacteria are adopted by stem leaves of the hybrid broussonetia papyriferaFermenting yeast and Bacillus bifidus at a ratio of 1:1, every 1085Kg of stem and leaf of the broussonetia papyrifera is added into the CFU/g of strain; the corn cob is fermented by Bacillus bifidus and Lactobacillus acidophilus at a ratio of viable bacteria of 1:1, every 106Adding 3Kg of corncob into the CFU/g unit strain; fermenting stem and leaf of mulberry with Bacillus bulgaricus and Streptococcus thermophilus at viable ratio of 1:1, every 107Adding 3Kg of mulberry stems and leaves into CFU/g of strain; the stem and leaf of rhizoma Bletillae and the stem and leaf of radix aucklandiae are fermented with Bacillus bifidus and Streptococcus lactis at viable bacteria ratio of 1:1, 10 times8CFU/g unit strain is added with 1Kg of bletilla striata stem and leaf and 2Kg of costus root stem and leaf.
Example 3
This embodiment is substantially the same as embodiment 1 except that: in step 2, the stem and leaf of the hybrid paper mulberry are fermented by Bulgaria bacteria and bifidobacteria with viable bacteria ratio of 1:1, and each 1085Kg of stem and leaf of the broussonetia papyrifera is added into the CFU/g of strain; the corn cob is fermented by Bacillus bifidus and Lactobacillus acidophilus with viable bacteria ratio of 1:1, 10 times7Adding 3Kg of corncob into the CFU/g of strain; fermenting stem and leaf of mulberry with yeast and Streptococcus thermophilus at viable ratio of 1:1, every 108Adding 3Kg of mulberry stems and leaves into CFU/g of strain; the stem and leaf of rhizoma Bletillae and the stem and leaf of radix aucklandiae are fermented by Streptococcus thermophilus and Streptococcus lactis at viable bacteria ratio of 1:1, 10 times7CFU/g strain is added with 1Kg of bletilla striata stem and leaf and 2Kg of costus root stem and leaf.
Example 4
This embodiment is substantially the same as embodiment 1 except that: in step 2, fermenting stem and leaf of the hybrid broussonetia papyrifera with streptococcus thermophilus and bifidobacterium in viable bacteria ratio of 1:1, wherein 10 times8Adding 5Kg of stem and leaf of the broussonetia papyrifera into CFU/g unit strain; the corn core is fermented by Streptococcus thermophilus and Lactobacillus acidophilus with viable bacteria ratio of 1:1, every 107Adding 3Kg of corn core into the CFU/g unit strain; the stem and leaf of mulberry are fermented by Bulgaria bacteria and Streptococcus thermophilus with viable bacteria ratio of 1:1, 10 times6Adding 3Kg of mulberry stems and leaves into CFU/g unit strain; the stem and leaf of rhizoma Bletillae and the stem and leaf of radix aucklandiae are fermented with yeast and Streptococcus lactis at viable bacteria ratio of 1:1, 10 times7CFU/g unit strain is added with 1Kg of bletilla striata stem and leaf and 2Kg of costus root stem and leaf.
Polysaccharides, flavones, crude proteins, crude fats, soluble fibers and soluble carbohydrates in the fermentates obtained in step 2 of examples 1-4 were measured and evaluated for acidity and palatability, with the results shown in table 2.
Table 2 results of detection of each of the fermented products obtained in examples 4 to 6
Figure BDA0003370317250000061
Comparison shows that each fermented product obtained in example 1 has moderate acidity and good palatability; examples 2-4 each of the fermentations obtained had an acidity pH in the range of 3.8-6.7, but had poor palatability and contained less of each nutrient than in example 1. Therefore, the fermentation bacteria of the stem and leaf of the hybrid paper mulberry of the invention select lactobacillus acidophilus and bifidobacterium; the fermentation bacteria of the corncob are saccharomycetes and lactobacillus acidophilus; the fermentation bacteria of mulberry stems and leaves select bifidobacterium and streptococcus thermophilus; the fermentation bacteria of the stem and leaf of bletilla striata and the stem and leaf of aucklandia root are selected from Bulgaria bacillus and streptococcus lactis. The protein content of the stem and leaf of the hybrid broussonetia papyrifera is high, the bifidobacterium can decompose alpha-casein through phosphoprotein phosphatase to promote protein absorption, and the lactobacillus acidophilus can further ferment carbohydrate in the stem and leaf of the broussonetia papyrifera to generate lactic acid so as to promote the digestion and absorption of the protein; the content of protein and acid components in mulberry stems and leaves is high, the bifidobacterium can decompose alpha-casein through phosphoprotein phosphatase to promote protein absorption, the acid resistance of streptococcus thermophilus is strong, and L-lactic acid and folic acid are generated in the fermentation process; the corn cob has high sugar content, the saccharomycete is a facultative anaerobic microorganism and can ferment and decompose sugar into alcohol and carbon dioxide, and the lactobacillus acidophilus further produces lactic acid by fermenting carbohydrate in the corn cob; the stem and leaf of bletilla striata and the stem and leaf of aucklandia root contain aromatic components, the content of lactic acid and acetic acid can be increased in the fermentation process of streptococcus lactis, the bacillus bulgaricus can decompose saccharides to generate a large amount of lactic acid, and diacetyl and acetaldehyde are generated in the fermentation process to generate unique flavor.
Example 5
This embodiment is substantially the same as embodiment 1 except that: in the step 2, the ratio of live bacteria in the using bacteria is 2: 1.
Example 6
This embodiment is substantially the same as embodiment 1 except that: in the step 2, the ratio of live bacteria in the using bacteria is 1: 2.
Polysaccharides, flavones, crude proteins, crude fats, soluble fibers and soluble carbohydrates in the fermentates obtained in step 2 of examples 5-6 were measured and evaluated for acidity and palatability, and the results are shown in table 1.
TABLE 1 results of detection of each of the fermented products obtained in examples 1 to 3
Figure BDA0003370317250000071
Comparison shows that each fermentation obtained in example 5 has moderate acidity but poor palatability; the acidity of each fermentation product obtained in the example 6 is higher, the palatability is poorer, and the effect is poorer than that of the fermentation product obtained in the example 1; and the content of each nutrient component is lower than that of the example 1.
Example 7
1) 5Kg of stem and leaf of the broussonetia papyrifera, 3Kg of corncob, 1Kg of stem and leaf of bletilla striata, 3Kg of stem and leaf of mulberry and 2Kg of stem and leaf of costus root are taken and respectively crushed into particles with the particle size of 1 cm.
2) Mixing the above pulverized stem and leaf of Broussonetia papyrifera, corncob, stem and leaf of bletilla striata, stem and leaf of mulberry and stem and leaf of radix aucklandiae uniformly, fermenting with Lactobacillus acidophilus, Bifidobacterium, Streptococcus thermophilus, yeast, Bacillus bulgaricus and Streptococcus lactis at viable bacteria ratio of 1:1:1:1:1:1, wherein the strain is 10 times8Adding 10Kg of CFU/g, and fermenting at 25-35 deg.C for 12-24 h.
3) Pulverizing Os Sepiae to 200 mesh to obtain Os Sepiae micropowder.
4) The functional feed additive is prepared by uniformly mixing 1kg of fermentation product of stem and leaf of hybrid paper mulberry, 1kg of fermentation product of corncob, 1kg of fermentation product of stem and leaf of bletilla striata, 1kg of fermentation product of stem and leaf of mulberry, 1kg of fermentation product of stem and leaf of costus root and 1kg of micro powder of cuttlebone.
Examples 8 to 14
The embodiments 8 to 14 are basically the same as the embodiment 7, except that: in the step 3, the mass fraction ratios of the broussonetia papyrifera stem and leaf fermented product, the corncob fermented product, the bletilla striata stem and leaf fermented product, the mulberry stem and leaf fermented product, the elecampane stem and leaf fermented product and the cuttlebone are respectively 2:2:1:2:2, 3:3:1:2:2, 4:3:1:2:2, 5:3:1:3:2:2, 6:3:1:3:2:2, 7:3:1:3:2:2 and 8:4:2:3:2:2 (taking 1kg of the broussonetia papyrifera stem and leaf fermented product as a reference).
Example 15 prescription Effect test
(1) Purpose of the experiment
The functional feed additive of the embodiment 7-14 is selected according to the modern breeding condition and the growth rule of the lambs in the test, the lambs are tested and screened, the lamb weight gain, the excrement forming rate and the like are used as indexes, the treatment condition of the traditional Chinese medicine formula on the lamb diarrhea is observed, and finally the formula with exact effect is screened, so that the foundation is laid for later research.
(2) Test method
For the lambs 160 with diarrhea in the weaning period (90 +/-10 days old), the diarrhea lambs are divided into 16 groups according to the weight, and the formulas of the functional feed additives of the groups 1 to 8 are the example 7 to the example 14. The 9 th group is 6 components with a ratio of 0:1:1:1:1:1, the 10 th group is 6 components with a ratio of 1:0:1:1:1, the 11 th group is 6 components with a ratio of 1:1:0:1:1, the 12 th group is 6 components with a ratio of 1:1:1:0:1:1, the 13 th group is 6 components with a ratio of 1:1:1:1:0:1, the 14 th group is 6 components with a ratio of 1:1:1:1:1:0, the 15 th group is an antibiotic treatment group (gentamicin) and a 16 th group morbidity control group, the administration amount of each group is 0.5g/kg body weight, 10 days after administration, diarrhea conditions are counted, whether drinking water, food intake, exercise and the like are normal or not is recorded and observed every day, and the cure rate and death rate of each group are comprehensively compared at the end of the test.
(3) Test results and analysis
TABLE 3 lamb diarrhea statistics for each group
Figure BDA0003370317250000091
Figure BDA0003370317250000101
As can be seen from Table 3, the clinical effects of the functional feed additives of examples 7 to 14 on lamb diarrhea are better than those of the empty group, the lamb stool forming numbers of examples 10 and 11 are higher than that of gentamicin, and the cure rate of example 11 is the highest; a comparison test (9 th to 14 th groups) that a certain component is lacked shows that the broussonetia papyrifera stem and leaf leavening and the bletilla striata stem and leaf leavening are key components. Therefore, the stem and leaf fermentation product of the broussonetia papyrifera is selected: corn cob fermentation product: fermentation of bletilla striata stem and leaf: fermentation of mulberry stems and leaves: fermentation product of woody stems and leaves: the cuttlebone is used as the optimal formula proportion of the functional feed additive according to the mass ratio of 5:3:1:3:2: 2.
EXAMPLE 16 pharmacodynamic study
(1) Test method
The diarrhea lambs of 30 lambs with diarrhea in the weaning period (90 +/-10 days old) are divided into 3 groups according to the weight, namely a functional feed additive group, an antibiotic treatment group (gentamicin) and a control group which are obtained in example 11 respectively, the administration amount of each group is 0.5g/kg of the weight, 10 days are observed after 5 days of administration, the diarrhea morbidity is counted, whether drinking water, food intake, exercise and the like are normal every day is recorded and observed, and the cure rate and the death rate of each group are comprehensively compared when the test is finished.
(2) Test results and analysis
TABLE 4 lamb diarrhea statistics for each group
Figure BDA0003370317250000102
Figure BDA0003370317250000111
In the test process, only 1 lamb dies in the control group, as can be seen from table 4, the clinical effect of the functional feed additive obtained by the invention on lamb diarrhea treatment is obviously better than that of antibiotic treatment, the average weight and the stool formation of the lambs are higher than those of gentamicin and the control group, the lamb skin and hair of the functional feed additive group are bright, the health state is good, and the functional feed additive provided by the invention is simultaneously shown to be capable of obviously improving the cure rate of lamb diarrhea.
The above-mentioned embodiments are merely preferred embodiments of the present invention, which are merely illustrative and not restrictive, and it should be understood that various changes and modifications may be made by those skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.

Claims (8)

1. A functional feed additive is characterized by being prepared from the following raw materials in parts by weight: 3-5 parts of a broussonetia papyrifera stem and leaf fermented product, 2-3 parts of a corncob fermented product, 1-2 parts of a bletilla striata stem and leaf fermented product, 2-4 parts of a mulberry stem and leaf fermented product, 2-3 parts of an elecampane stem and leaf fermented product and 1-2 parts of cuttlebone.
2. The functional feed additive according to claim 1, which is prepared from the following raw materials in parts by weight: 5 parts of a fermentation product of stem and leaf of the broussonetia papyrifera, 3 parts of a fermentation product of corncob, 1 part of a fermentation product of stem and leaf of bletilla striata, 3 parts of a fermentation product of stem and leaf of mulberry, 2 parts of a fermentation product of stem and leaf of elecampane and 2 parts of cuttlebone.
3. The functional feed additive according to claim 1 or 2, wherein the control indexes of the additive are as follows: the polysaccharide content is not less than 9.3%, the flavone content is not less than 6.1%, the crude protein content is not less than 17.4%, the crude fat content is not less than 5.6%, the soluble fiber content is not less than 16.9%, and the soluble carbohydrate content is not less than 23.5%.
4. The functional feed additive as claimed in claim 1 or 2, wherein the broussonetia papyrifera stem and leaf fermented product is prepared by fermenting broussonetia papyrifera stem and leaf with lactobacillus acidophilus and bifidobacterium; the corn cob ferment is prepared by fermenting corn cobs with saccharomycetes and lactobacillus acidophilus; the mulberry stem and leaf fermented product is prepared by fermenting mulberry stem and leaf with bifidobacterium and streptococcus thermophilus; the bletilla striata stem and leaf fermented product and the costus stem and leaf fermented product are prepared by fermenting bletilla striata stem and leaf and costus stem and leaf with bacillus bulgaricus and streptococcus lactici.
5. The functional feed additive as claimed in claim 1 or 2, wherein the broussonetia papyrifera stem and leaf fermented product, the corncob fermented product, the bletilla striata stem and leaf fermented product, the mulberry stem and leaf fermented product and the elecampane stem and leaf fermented product are prepared by mixing and fermenting broussonetia papyrifera stem and leaf, corncob, bletilla striata stem and leaf, mulberry stem and leaf and elecampane stem and leaf with lactobacillus acidophilus, bifidobacterium, streptococcus thermophilus, saccharomycetes, bulgaricus and streptococcus lactis.
6. A method for preparing a functional feed additive according to any one of claims 1, 2 and 4, comprising the steps of:
1) respectively pulverizing stem and leaf of Broussonetia papyrifera, corn cob, stem and leaf of rhizoma Bletillae, stem and leaf of mulberry, and stem and leaf of radix aucklandiae to particles with particle size of 1 cm;
2) taking the crushed stem and leaf of the hybrid paper mulberry, fermenting by using lactobacillus acidophilus and bifidobacterium with the viable bacteria ratio of 1:1, fermenting the corncob by using saccharomycetes and lactobacillus acidophilus with the viable bacteria ratio of 1:1, fermenting the stem and leaf of the mulberry by using bifidobacterium and streptococcus thermophilus with the viable bacteria ratio of 1:1, and fermenting the stem and leaf of the bletilla striata and the stem and leaf of the elecampane by using bulgaria and streptococcus lactis with the viable bacteria ratio of 1: 1;
3) pulverizing Os Sepiae to 200 mesh to obtain Os Sepiae micropowder;
4) mixing the stem and leaf fermented product of Broussonetia papyrifera, the corn cob fermented product, the stem and leaf fermented product of rhizoma Bletillae, the stem and leaf fermented product of mulberry, the stem and leaf fermented product of radix aucklandiae and Os Sepiae micropowder.
7. A method for preparing a functional feed additive according to any one of claims 1, 2 and 5, comprising the steps of:
1) respectively pulverizing stem and leaf of Broussonetia papyrifera, corn cob, stem and leaf of rhizoma Bletillae, stem and leaf of mulberry, and stem and leaf of radix aucklandiae to particles with particle size of 1 cm;
2) uniformly mixing crushed stem and leaf of the hybrid paper mulberry, corncob, stem and leaf of bletilla striata, stem and leaf of mulberry and stem and leaf of costus root, and fermenting by lactobacillus acidophilus, bifidobacterium, streptococcus thermophilus, saccharomycete, lactobacillus bulgaricus and streptococcus lactis with viable bacteria ratio of 1:1:1:1: 1;
3) pulverizing Os Sepiae to 200 mesh to obtain Os Sepiae micropowder;
4) mixing the stem and leaf fermented product of Broussonetia papyrifera, the corn cob fermented product, the stem and leaf fermented product of rhizoma Bletillae, the stem and leaf fermented product of mulberry, the stem and leaf fermented product of radix aucklandiae and Os Sepiae micropowder.
8. Use of the functional feed additive according to claim 1 or 2 for the preparation of a medicament or feed for the prevention or treatment of ruminant diarrhoea.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115039839A (en) * 2022-06-24 2022-09-13 河南牧业经济学院 Traditional Chinese medicine feed additive for improving mutton quality and enhancing mutton aroma and preparation method thereof

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102232467A (en) * 2011-04-15 2011-11-09 北京康华远景科技有限公司 Livestock and poultry anti-diarrheal feed additive and preparation method thereof
CN103355552A (en) * 2013-08-05 2013-10-23 山东新希望六和集团有限公司 Mixed feed for carp in fingerling stage and preparation method thereof
CN104366013A (en) * 2014-10-21 2015-02-25 广西平果利华茧丝绸有限公司 Method for preparing biological feed by taking stems and leaves of mulberry as main raw materials
CN106954756A (en) * 2017-04-14 2017-07-18 广东禾欣构树产业发展有限公司 A kind of broiler fodder for adding fermentation hybridization paper mulberry powder and application thereof
CN107373022A (en) * 2017-09-11 2017-11-24 广东省农业科学院蚕业与农产品加工研究所 Pig feed
CN112266883A (en) * 2020-10-26 2021-01-26 中商构能生态科技(天津)有限公司 Zymophyte liquid for whole-plant fermentation of hybrid paper mulberry, and preparation method and application thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108402317A (en) * 2018-05-30 2018-08-17 金寨县绿晨生态农业开发有限公司 A kind of feed and preparation method thereof for treating lamb diarrhoea
CN109589384A (en) * 2019-02-01 2019-04-09 肖月华 A kind of pharmaceutical composition for gastric ulcer gastritis and preparation method thereof and purposes

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102232467A (en) * 2011-04-15 2011-11-09 北京康华远景科技有限公司 Livestock and poultry anti-diarrheal feed additive and preparation method thereof
CN103355552A (en) * 2013-08-05 2013-10-23 山东新希望六和集团有限公司 Mixed feed for carp in fingerling stage and preparation method thereof
CN104366013A (en) * 2014-10-21 2015-02-25 广西平果利华茧丝绸有限公司 Method for preparing biological feed by taking stems and leaves of mulberry as main raw materials
CN106954756A (en) * 2017-04-14 2017-07-18 广东禾欣构树产业发展有限公司 A kind of broiler fodder for adding fermentation hybridization paper mulberry powder and application thereof
CN107373022A (en) * 2017-09-11 2017-11-24 广东省农业科学院蚕业与农产品加工研究所 Pig feed
CN112266883A (en) * 2020-10-26 2021-01-26 中商构能生态科技(天津)有限公司 Zymophyte liquid for whole-plant fermentation of hybrid paper mulberry, and preparation method and application thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
程衷逸编著: "《兽医中药类编》", 31 July 1957, 财政经济出版社, pages: 30 - 31 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115039839A (en) * 2022-06-24 2022-09-13 河南牧业经济学院 Traditional Chinese medicine feed additive for improving mutton quality and enhancing mutton aroma and preparation method thereof

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