Summary of the invention
In order to adapt to the needs at vast breeding layer chicken family better, improve the economic benefit that laying hen produces, the invention provides a kind of raising laying hen production capacity and autoimmunity, improve food conversion ratio, and then minimizing medicament residue, raising egg quality, the dysgenic new type of safe that the production of reduction laying hen causes human and environment is reliable, environmental protection composite biological feed additive product.
Another object of the present invention is to provide the preparation method of this composite biological feed additive.
The object of the invention is to realize by following technical solution:
The invention provides a kind of laying hen composite biological feed additive, this additive is a kind of pulvis, and its raw materials comprises following constituent: feed grade fulvic acid, worm powder, through the worm excrement of fermentation, and extract complex preparation, probiotics and carrier; Wherein, according to percentage by weight, feed grade fulvic acid 25% ~ 30%, worm powder 10% ~ 15%, through the worm excrement 15% ~ 30% of fermentation; Extract complex preparation 2% ~ 5%, probiotics 3% ~ 5%, stone flour carrier 25% ~ 30%; Each weight percentages of components sum is 100%.
Further, described extract complex preparation raw material consists of Astragalus Root P.E, Radix Pseudostellariae extract and grape seed extract; Described probiotics consists of bacillus subtilis, saccharomyces cerevisiae and Plant Lactobacilli.
Further, in described extract complex preparation, each raw material is 2:2:1 by the weight ratio of Astragalus Root P.E, Radix Pseudostellariae extract and grape seed extract, is prepared into described extract complex preparation.
Further, in described probiotics composition, bacillus subtilis, saccharomyces cerevisiae and Plant Lactobacilli are that 3:2:3 compound mixture forms according to weight ratio, wherein total viable count > 1.6 × 10
9cfug
-1.
Further, described worm powder is one in barley worm dry powder, yellow meal worm dry powder or its mixture, and described worm excrement is one in the barley worm worm excrement, yellow meal worm worm excrement of fermentation or its mixture.
Further, the percentage by weight that described laying hen composite biological feed additive adds in egg feedstuff is 1 ~ 5 ‰.
Preferably, this additive mixes primarily of the composition of following weight percentage: feed grade fulvic acid 30%, worm powder 10%, through the worm excrement 25% of fermentation; Extract complex preparation 5%, probiotics 3%, stone flour carrier 27%.
Above-mentioned additive first makes powder before being added into egg feedstuff, sieves, and stirs, be 1 ‰ ~ 5 ‰ add in basal diet according to percentage by weight, improve laying hen production capacity and autoimmunity, improve food conversion ratio, and then minimizing medicament residue, improve egg quality.
Wherein, fulvic acid of the present invention uses commercially available feed grade fulvic acid product, and main component fulvic acid mass percentage is greater than 40%;
Another object of the present invention is the preparation method providing a kind of laying hen composite biological feed additive, it is characterized in that: described preparation method comprises:
(1) extract complex preparation is prepared: produce Astragalus Root P.E, Radix Pseudostellariae extract and grape seed extract respectively, be 2:2:1 by obtained extract complex preparation by the weight ratio of Astragalus Root P.E, Radix Pseudostellariae extract and grape seed extract, be prepared into described extract complex preparation;
(2) microorganism formulation is prepared;
(3) preparation of fermentation worm excrement;
(4) by the percentage by weight of described raw material by stone flour carrier, feed grade fulvic acid, worm powder, after the worm excrement of fermentation, extract complex preparation and probiotics in proportion proportioning, put into mixer, mix, pulverize, stir, to obtain final product.
Further, described raw material all adopted 60 mesh sieves, was prepared into pulvis.
Further, the preparation of described extract complex preparation comprises:
(1) preparation of Astragalus Root P.E: Radix Astragali impurity elimination, chopping process, add the water soaking 4 hours of 8 ~ 10 times amount volumes, refluxing extraction 2 hours, collecting by filtration filtrate, again adds the water refluxing extraction 2 hours of 5 times amount volumes in filter residue, collecting by filtration filtrate, discard filter residue merging filtrate, be then separated with large pore resin absorption column, wash with water, collect eluent, after concentrated, evaporate to dryness obtains Astragalus Root P.E powder;
(2) preparation of Radix Pseudostellariae extract: radix pseudostellariae impurity elimination, grind into powder, then the water soaking 5 hours of 8 ~ 10 times amount volumes is added, refluxing extraction 2 hours, collecting by filtration filtrate again adds the water of 5 times amount volumes in filter residue, continue backflow 2 hours, collecting by filtration filtrate, merges twice filtrate, and after concentrated, evaporate to dryness obtains Radix Pseudostellariae extract powder;
(3) by after obtained Astragalus Root P.E powder and the mixing of Radix Pseudostellariae extract powder, add pulverous grape seed extract in proportion, stir and evenly mix obtained.
Further, described probiotics is fermented separately by bacillus subtilis, saccharomyces cerevisiae and Plant Lactobacilli, and final drying is that 3:2:3 compound mixture forms according to weight ratio, wherein total viable count > 1.6 × 10
9cfug
-1.
Further, the preparation method of the described worm excrement through fermentation is:
(1) getting after worm excrement crosses 100 mesh sieves pulverizes as raw material, and dregs of beans addition is 10% ~ 15% of worm excrement, and sugared addition is 0.5% ~ 1% of worm excrement, and the amount adding pure water after mixing is 5% ~ 10% of total amount, is again uniformly mixed; Select Lactobacillus plantarum, bacillus is in 1:1 ratio as leavening, and inoculum concentration accounts for 0.3% ~ 0.5% of worm excrement; Fermentation vat or fermenter are put in above-mentioned supplementary material mixing, carries out anaerobic fermentation; Fermentation temperature is ferment between 30 DEG C ~ 35 DEG C; Fermentation time is 24-48 hour;
(2) learnt from else's experience the worm excrement of first time fermentation, adding the amount of salt is 0.5% ~ 1% of worm excrement, and the amount of adding sugar is 0.5% ~ 1% of worm excrement; Select lactobacillus acidophilus, saccharomycete, bacillus is as fermenting agent; Wherein, lactic acid bacteria, saccharomycete, the ratio of bacillus is 1:1:1-2:2:1, and inoculum concentration accounts for 0.3% ~ 0.6% of worm excrement; Fermentation vat or fermenter are put in above-mentioned supplementary material mixing, carries out second time cold fermentation; When filling with substance notices that charging is loose; Otherwise flora reproductive effect can be affected, install rear cap seal jail.Fermentation temperature must ferment between 20 DEG C ~ 30 DEG C; Fermentation time is 24-36 hour;
(3) the worm excrement after secondary fermentation is after spraying dry, and 80 mesh sieves obtain the worm excrement through fermentation excessively.
Adding dregs of beans in step 1 can make fermentation worm excrement material comparatively loose, ensures that ferment effect can make fermentation increase the content of plant crude protein more fully simultaneously, and adopts Bacillus acidi lactici to coordinate bacillus as leavening; Wherein, the viable count of lactobacillus plantarum is higher, product acid that can be a large amount of, and the content of beary metal in the acid mass-energy degraded worm excrement of its output, worm excrement is played to the double effects of fermentation and purification; And Lactobacillus plantarum can output distinctive lactobacillin be the anticorrisive agent of a kind of bion in reproductive process, the worm excrement after fermentation is played and anticorrosionly prevents peculiar smell generation effect, be conducive to the stability of this additive simultaneously; Bacillus can suppress the growth and breeding of the harmful microbe such as harmful bacteria, pathogen during the fermentation, and decomposes the effect that organic matter-water-organic matter matter, organic sulfur compound, organic nitrogen etc. play antibacterial deinsectization excrement peculiar smell; And bacillus can produce abundant metabolism product, as: synthesize the materials such as multiple organic acid, enzyme, physiologically active, and other multiple nutrient be easily utilized.
Add sugar and salt in step 2, and select lactobacillus acidophilus, saccharomycete, bacillus, as fermenting agent, carries out secondary fermentation further, can make worm manure fermentation more completely with abundant, and remove the peculiar smell of worm excrement further, make the fermentation worm excrement sweet mouthfeel obtained, free from extraneous odour; More easily absorb; And probiotics can be retained in the worm excrement of fermentation by the interpolation of leavening and fermentation, the production performance of laying hen can be improved while can digestion and absorption function being promoted, improve egg quality.
Further, described feed grade fulvic acid is provided by NeiMengGuYongYe Biology Technology Co., Ltd, with natural weathered coal and brown coal for raw material, utilize the natural fulvic acid product that modern biotechnology is highly purified, its main component fulvic acid mass percentage is greater than 40%.
Composite biological feed additive of the present invention, first makes powder before being added into egg feedstuff, and any existing method can be adopted to be prepared, and normally takes each component materials and pulverizes, sieve, stir.
In order to improve the absorptivity of laying hen to composite biological feed additive, improve performance in layers, improve egg quality, control effectively to the particle diameter of pre-mixing agent, the powder in above-mentioned preparation method all adopted 60 mesh sieves.
It is 1 ~ 5 ‰ for good that composite biological feed additive provided by the invention adds percentage by weight in layer diets, is 0.5% for best with percentage by weight.
Beneficial effect of the present invention is:
(1) fulvic acid used in the present invention is a kind of organic monoacid containing aromatic structure, containing more active group, the activity of multiple enzyme can be activated, strengthen cellular respiration, promote tissue metabolism, regulation of physiological functions, to the metabolism of adjustment chicken body, improving performance in layers has obvious effect;
(2) the worm excrement added in the present invention is the worm excrement through fermentation, worm excrement by fermentation eliminates the original smell of worm sand, worm excrement smell after fermentation is comparatively fragrant, mouthfeel is good, and make the biological function flora added (as the Lactobacillus plantarum added in one time fermentation, the lactobacillus acidophilus that bacillus and second time fermentation are added, saccharomycete etc.) amount reproduction, more benefit after using and assimilate, add the content that can improve crude protein and mineral matter in egg feedstuff simultaneously to.And its nutritive value is that its rich in nutrition content and bioactivator are comparatively comprehensive, and in worm excrement, itself is containing a large amount of polypide metabolites and secretion, and bioactivator wherein may have facilitation to the metabolism of animal; The laying hen organism metabolism ability of laying period can be improved, promote its absorption to the nutrient such as peptide, amino acid and the synthesis of protein, improve Egg Quality.And carry out regeneration as raw material, ferment worm excrement safety, the health made, achieve agriculture and to give up the recycling of resource, while economizing on resources, reduce cost.
(3) extract complex preparation used in the present invention is mainly astragalus polyose, grape seed extract, Radix Pseudostellariae extract etc., wherein astragalus polyose has antiviral and to immune organ regulating action, the Immune Organs Index such as animal thymus and spleen can be improved, strengthen the phagocytic function of reticuloendothelial system, blood middle leukocytes and monocyte quantity are significantly increased, make macrophage phagocytic percentage and phagocytic index significantly increase, strengthen the function of Cellular Immunity and humoral immunity.Radix Pseudostellariae extract has the appetite of shaking that helps digestion, and coordinates strengthen antiviral and strengthen immune function with the Radix Astragali.Grape seed extract has high-efficiency antioxidant effect, the free radical in purged body that can be potent, has very strong activity in vivo, and absorbs rapidly completely; The immunity greatly improving unit can be crossed; Simultaneously grape seed extract also has antiinflammatory action, the effect that anti-allergic effects is strong, and non-toxic and safe, and three works in coordination with can by while playing its immunoregulation effect to the impact of animal immune organ; Improve performance in layers and egg quality.
(4) probiotics used in the present invention forms by bacillus subtilis, saccharomyces cerevisiae and Plant Lactobacilli are composite, wherein total viable count > 1.6 × 10
9cfug
-1, not only have strengthening animal immune system, selective promotion animal intestinal beneficial bacterium propagation, suppresses the field planting of beneficial bacterium and breeds, improving microbial environment in animal body, stimulating immune response, strengthens immunity of livestock, improves livestock and poultry production performance.
(5) composite biological feed additive in the present invention be by fulvic acid, extract complex preparation, the worm excrement through fermentation, worm powder, compound is formulated according to a certain percentage for probiotics and other carriers, cooperatively interact and jointly play a role together.The conbined usage of each component, shows good synergistic action effect in laying hen produces.The premix of this composite biological feed additive is used in egg feedstuff, not only can regulate and control livestock and poultry alimentary tract environment, regulate body gut flora balance, enhanced machine body immunity function, there is antibacterial, resisting stress, develop immunitypty, raising production performance, and utilize the nutriment, enzyme, metabolite etc. of different flora itself to improve the price of deed, improve animal products quality, also have that addition is few, preparation technology is simple, and Long-Time Service noresidue, without the resistance to the action of a drug, the advantage such as have no side effect, market application foreground is good.
Detailed description of the invention
In the following embodiments, the invention discloses the specific implementation of described composite biological feed additive in egg feedstuff, only for illustration of more excellent implementation of the present invention, but not formation is particularly limited to.
Embodiment 1: the preparation of composite biological feed additive
The preparation of A, feed grade fulvic acid: provided by NeiMengGuYongYe Biology Technology Co., Ltd, with natural weathered coal and brown coal for raw material, utilize the natural fulvic acid product that modern biotechnology is highly purified, its main component fulvic acid mass percentage content is greater than 40%.Fulvic acid is a kind of organic monoacid containing aromatic structure, containing more active group, can activate the activity of multiple enzyme, strengthen cellular respiration, promote tissue metabolism, regulation of physiological functions, to the metabolism of adjustment chicken body, improving performance in layers has obvious effect.
The preparation of B, extract complex preparation:
(1) preparation of Astragalus Root P.E: Radix Astragali impurity elimination, chopping process, add the water soaking 4 hours of 8 times amount volumes, refluxing extraction 2 hours, collecting by filtration filtrate, again add in filter residue 5 times amount volumes water refluxing extraction 2 hours, collecting by filtration filtrate, discards filter residue merging filtrate, is then separated with large pore resin absorption column, wash with water, collect eluent, evaporate to dryness after concentrated, obtains astragalus polyose (APS) powder;
(2) preparation of Radix Pseudostellariae extract: radix pseudostellariae impurity elimination, grind into powder, then the water soaking 5 hours of 8 times amount volumes is added, refluxing extraction 2 hours, collecting by filtration filtrate again adds the water of 5 times amount volumes in filter residue, continue backflow 2 hours, collecting by filtration filtrate, merges twice filtrate, evaporate to dryness powder after concentrated;
(3) by after obtained Astragalus Root P.E powder and the mixing of Radix Pseudostellariae extract powder, Astragalus Root P.E, Radix Pseudostellariae extract and grape seed extract are 2:2:1 by weight, add pulverous grape seed extract, stir and evenly mix obtained extract complex preparation.Wherein grape seed extract is purchased from market, its procyanidin content >=95%.
C, the preparation of worm excrement through fermentation:
(1) getting after worm excrement crosses 100 mesh sieves pulverizes as raw material, and dregs of beans addition is 10% of worm excrement, and sugared addition is 1% of worm excrement, and the amount adding pure water after mixing is 6% of total amount, is again uniformly mixed; Select Lactobacillus plantarum, bacillus is in 1:1 ratio as leavening, and inoculum concentration accounts for 0.3% of worm excrement; Fermentation vat or fermenter are put in above-mentioned supplementary material mixing, carries out anaerobic fermentation; Fermentation temperature is ferment between 30 DEG C ~ 35 DEG C; Fermentation time is 32 hours.Wherein adding dregs of beans can make fermentation worm excrement material comparatively loose, ensures that ferment effect can make fermentation increase the content of plant crude protein more fully simultaneously, and adopts Bacillus acidi lactici to coordinate bacillus as leavening; Wherein, the viable count of lactobacillus plantarum is higher, product acid that can be a large amount of, and the content of beary metal in the acid mass-energy degraded worm excrement of its output, worm excrement is played to the double effects of fermentation and purification; And Lactobacillus plantarum can output distinctive lactobacillin be the anticorrisive agent of a kind of bion in reproductive process, the worm excrement after fermentation is played and anticorrosionly prevents peculiar smell generation effect, be conducive to the stability of this additive simultaneously; Bacillus can suppress the growth and breeding of the harmful microbe such as harmful bacteria, pathogen during the fermentation, and decomposes the effect that organic matter-water-organic matter matter, organic sulfur compound, organic nitrogen etc. play antibacterial deinsectization excrement peculiar smell; And bacillus can produce abundant metabolism product, as: synthesize the materials such as multiple organic acid, enzyme, physiologically active, and other multiple nutrient be easily utilized.
(2) learnt from else's experience the worm excrement of first time fermentation, adding the amount of salt is 0.5% of worm excrement, and the amount of adding sugar is 1% of worm excrement; Select lactobacillus acidophilus, saccharomycete, bacillus is as fermenting agent; Wherein, lactic acid bacteria, saccharomycete, the ratio of bacillus is 1:1:1, and inoculum concentration accounts for 0.6% of worm excrement; Fermentation vat or fermenter are put in above-mentioned supplementary material mixing, carries out second time cold fermentation; When filling with substance notices that charging is loose; Otherwise flora reproductive effect can be affected, install rear cap seal jail.Fermentation temperature must ferment between 20 DEG C ~ 30 DEG C; Fermentation time is 36 hours.Wherein add sugar and salt, and select lactobacillus acidophilus, saccharomycete, bacillus, as fermenting agent, carries out secondary fermentation further, can make worm manure fermentation more completely with abundant, and remove the peculiar smell of worm excrement further, make the fermentation worm excrement sweet mouthfeel obtained, free from extraneous odour; More easily absorb; And probiotics can be retained in the worm excrement of fermentation and amount reproduction by the interpolation of leavening and fermentation, add in egg feedstuff, can play and suppress the propagation of spoilage organisms to have antagonism to pathogenic microorganisms, the production performance of laying hen can be improved while promoting digestion and absorption function, improve egg quality.
(3) the worm excrement after secondary fermentation is after spraying dry, and 80 mesh sieves obtain the worm excrement through fermentation excessively.
The preparation of D, probiotics: fermented separately by bacillus subtilis, saccharomyces cerevisiae and plant lactobacillus, final drying is that 3:2:3 compound mixture forms according to weight ratio, wherein total viable count > 1.6 × 10
9cfug
-1.Not only have strengthening animal immune system, selective promotion animal intestinal beneficial bacterium propagation, suppresses the field planting of beneficial bacterium and breeds, improving microbial environment in animal body, stimulating immune response, strengthens immunity of livestock, improves livestock and poultry production performance.
Above-mentioned obtained raw materials by weight is by feed grade fulvic acid 30%, and worm powder 10%, through the worm excrement 25% of fermentation; Extract complex preparation 5%, probiotics 3%, stone flour carrier 27%, in proportion after proportioning, is put into mixer, is mixed, pulverizes, stirs, to obtain final product.
Composite biological feed additive of the present invention, first makes powder before being added into egg feedstuff, and any existing method can be adopted to be prepared, and normally takes each component materials and pulverizes, sieve, stir.
In order to improve the absorptivity of laying hen to composite biological feed additive, improving performance in layers, improving egg quality, the particle diameter of additive being control effectively, adopted 60 mesh sieves.
The worm excrement added in additive of the present invention is the worm excrement through fermentation, worm excrement by fermentation eliminates the original smell of worm sand, worm excrement smell after fermentation is comparatively fragrant, mouthfeel is good, and the biological function flora making to add (as the Lactobacillus plantarum added in one time fermentation, the lactobacillus acidophilus that bacillus and second time fermentation are added, saccharomycete etc.) amount reproduction, more benefit after using and assimilate, add the content that can improve crude protein and mineral matter in egg feedstuff simultaneously to.And its nutritive value is that its rich in nutrition content and bioactivator are comparatively comprehensive, and in worm excrement, itself is containing a large amount of polypide metabolites and secretion, and bioactivator wherein may have facilitation to the metabolism of animal; The laying hen organism metabolism ability of laying period can be improved, promote its absorption to the nutrient such as peptide, amino acid and the synthesis of protein, improve Egg Quality.And carry out regeneration as raw material, ferment worm excrement safety, the health made, achieve agriculture and to give up the recycling of resource, while economizing on resources, reduce cost.Coordinate extract complex preparation, wherein astragalus polyose has antiviral and to immune organ regulating action, the Immune Organs Index such as animal thymus and spleen can be improved, strengthen the phagocytic function of reticuloendothelial system, blood middle leukocytes and monocyte quantity are significantly increased, make macrophage phagocytic percentage and phagocytic index significantly increase, strengthen the function of Cellular Immunity and humoral immunity.Radix Pseudostellariae extract has the appetite of shaking that helps digestion, and coordinates strengthen antiviral and strengthen immune function with the Radix Astragali.Grape seed extract has high-efficiency antioxidant effect, the free radical in purged body that can be potent, has very strong activity in vivo, and absorbs rapidly completely; The immunity greatly improving unit can be crossed; Simultaneously grape seed extract also has antiinflammatory action, the effect that anti-allergic effects is strong, and non-toxic and safe, and three works in coordination with can by while playing its immunoregulation effect to the impact of animal immune organ; Improve performance in layers and egg quality.
Composite biological feed additive in the present invention be by fulvic acid, extract complex preparation, the worm excrement through fermentation, worm powder, compound is formulated according to a certain percentage for probiotics and other carriers, cooperatively interact and jointly play a role together.The conbined usage of each component, shows good synergistic action effect in laying hen produces.The premix of this composite biological feed additive is used in egg feedstuff, not only can regulate and control livestock and poultry alimentary tract environment, regulate body gut flora balance, enhanced machine body immunity function, there is antibacterial, resisting stress, develop immunitypty, raising production performance, and utilize the nutriment, enzyme, metabolite etc. of different flora itself to improve the price of deed, improve animal products quality, also have that addition is few, preparation technology is simple, and Long-Time Service noresidue, without the resistance to the action of a drug, the advantage such as have no side effect, market application foreground is good.
The preparation of embodiment 2, composite biological feed additive
The preparation of A, feed grade fulvic acid: provided by NeiMengGuYongYe Biology Technology Co., Ltd, with natural weathered coal and brown coal for raw material, utilize the natural fulvic acid product that modern biotechnology is highly purified, its main component fulvic acid mass percentage content is greater than 40%;
The preparation of B, extract complex preparation:
(1) preparation of Astragalus Root P.E: Radix Astragali impurity elimination, chopping process, add the water soaking 4 hours of 10 times amount volumes, refluxing extraction 2 hours, collecting by filtration filtrate, again add in filter residue 5 times amount volumes water refluxing extraction 2 hours, collecting by filtration filtrate, discards filter residue merging filtrate, is then separated with large pore resin absorption column, wash with water, collect eluent, evaporate to dryness after concentrated, obtains astragalus polyose (APS) powder;
(2) preparation of Radix Pseudostellariae extract: radix pseudostellariae impurity elimination, grind into powder, then the water soaking 5 hours of 10 times amount volumes is added, refluxing extraction 2 hours, collecting by filtration filtrate again adds the water of 5 times amount volumes in filter residue, continue backflow 2 hours, collecting by filtration filtrate, merges twice filtrate, evaporate to dryness powder after concentrated;
(3) by after obtained Astragalus Root P.E powder and the mixing of Radix Pseudostellariae extract powder, Astragalus Root P.E, Radix Pseudostellariae extract and grape seed extract are 2:2:1 by weight, add pulverous grape seed extract, stir and evenly mix obtained extract complex preparation.
C, the preparation of worm excrement through fermentation:
(1) getting after worm excrement crosses 100 mesh sieves pulverizes as raw material, and dregs of beans addition is 15% of worm excrement, and sugared addition is 0.6% of worm excrement, and the amount adding pure water after mixing is 10% of total amount, is again uniformly mixed; Select Lactobacillus plantarum, bacillus is in 1:1 ratio as leavening, and inoculum concentration accounts for 0.5% of worm excrement; Fermentation vat or fermenter are put in above-mentioned supplementary material mixing, carries out anaerobic fermentation; Fermentation temperature is ferment between 30 DEG C ~ 35 DEG C; Fermentation time is 48 hours;
(2) learnt from else's experience the worm excrement of first time fermentation, adding the amount of salt is 0.8% of worm excrement, and the amount of adding sugar is 0.5% of worm excrement; Select lactobacillus acidophilus, saccharomycete, bacillus is as fermenting agent; Wherein, lactic acid bacteria, saccharomycete, the ratio of bacillus is 2:2:1, and inoculum concentration accounts for 0.4% of worm excrement; Fermentation vat or fermenter are put in above-mentioned supplementary material mixing, carries out second time cold fermentation; When filling with substance notices that charging is loose; Otherwise flora reproductive effect can be affected, install rear cap seal jail.Fermentation temperature must ferment between 20 DEG C ~ 30 DEG C; Fermentation time is 24 hours;
(3) the worm excrement after secondary fermentation is after spraying dry, and 80 mesh sieves obtain the worm excrement through fermentation excessively.
The preparation of D, probiotics: fermented separately by bacillus subtilis, saccharomyces cerevisiae and plant lactobacillus, final drying is that 3:2:3 compound mixture forms according to weight ratio, wherein total viable count > 1.6 × 10
9cfug
-1.
Above-mentioned obtained raw materials by weight is by yellow for feed grade rotten 30%, and worm powder 10%, through the worm excrement 22% of fermentation; Extract complex preparation 4%, probiotics 4%, stone flour carrier 30 as one kind %, in proportion after proportioning, puts into mixer, mixes, pulverizes, stirs, to obtain final product.
Composite biological feed additive of the present invention, first makes powder before being added into egg feedstuff, and any existing method can be adopted to be prepared, and normally takes each component materials and pulverizes, sieve, stir.
In order to improve the absorptivity of laying hen to composite biological feed additive, improving performance in layers, improving egg quality, the particle diameter of additive being control effectively, adopted 60 mesh sieves.
Embodiment 3
Composite biological feed additive is on the impact of performance in layers and Egg Quality
Select 1500 blue brown laying hens in the healthy sea of 280 ages in days to be divided into 5 groups at random, often organize 5 repetitions, each repetition 60.Control group fed basal diet, test I, II, III group adds the mixing of 0.015% terramycin, 0.02% extract complex preparation, 0.1% probiotics and the worm excrement through fermentation respectively in basal diet, in test IV basal diet, premix 5% compound bio composite biological feed additive of the present invention replaces, and other are the same.Basal diet is that chicken house is prepared burden certainly, and control group adds certain brand 5% premix with test I, II, III group, test IV group employing same materials and formula, but the laying hen composite biological feed additive of the present invention of 5% of premix employing.Composition and trophic level are in table 1.Test period is one week preliminary trial period wherein, formal test phase 30d.When preliminary trial period starts, record the egg number of each repetition, and suitably adjust according to statistics, make control group and test group laying rate difference between preliminary trial period not remarkable.Experimental result as table 2, shown in table 3.
Table 1 basal diet composition and trophic level
Note: the main calcic of premix, phosphorus, salt, trace element, vitamin, amino acid etc.
In table 1, percentage composition % is mass percent.
Result of the test shows, test laying hen is good in the duration of test state of mind, honey stomach, survival rate 100%.Different additive on the impact of performance in layers in table 2.From table, day material consumpting ratio control group of test I, II and III group declines all to some extent, difference remarkable (p > 0.05), and compound bio composite biological feed additive IV group of the present invention comparatively control group decline 1.85% (p < 0.05).Test II, III group laying rate, average egg weight all increase than control group, IV group laying rate improves 4.7% (p < 0.05), I group laying rate reduces by 2.85% (p < 0.05) than control group, IV group average egg weight higher than control group by 1.8% (p < 0.05).Test II, III, IV group feedstuff-egg ratio comparatively control group decreases, II, III group does not reduce by 2.88%, 2.40% (p < 0.05) than contrast component, and IV group feedstuff-egg ratio comparatively control group reduces by 9.43% (p < 0.01).Test II, III, IV group rate of broken eggs, the reduction more remarkable in control group of secondary egg rate, II group rate of broken eggs, secondary egg rate do not reduce by 14.81% and 11.04% (p < 0.05) than contrast component, I group is not higher by 20.99% than contrast component, 12.88% (p < 0.05), IV group is not lower by 27.16% than contrast component, 23.31% (p < 0.01).The diarrhea rate of test I, II, III, IV group respectively lower than control group by 51.49%, 59.17%, 49.77%, 64.22%, difference all extremely remarkable (p < 0.01).
As seen from Table 3, fat content comparatively control group and I, II group significantly reduction in test III, IV group egg, IV group is control group low by 15.44% (p < 0.05) comparatively.In test II, III, IV group egg, cholesterol content ratio control group reduces by 29.78%, 24.67% and 34.86% respectively, difference all extremely significantly (p < 0.01), tests cholesterol content ratio control group high by 2.35% (p > 0.05) in I group egg.Content of ashes higher than control group by 7.81% (p > 0.05) in test I group egg, content of ashes higher than control group by 15.63%, 13.28% and 18.75% (p < 0.05) respectively in test II, III, IV group egg.The shell thickness of each test group and control group are without significant difference, but protein content higher than control group by 0.95% (p > 0.05) in test I group egg, protein content higher than control group by 10.5%, 11.06% and 22.43% (p < 0.05) respectively in test II, III, IV group egg.The result of this test proves: through the worm excrement of fermentation, extract complex preparation, fulvic acid, probiotics and compound premixing agent thereof all can improve the production performance of the blue brown laying hen in sea, improve egg quality, reduce the content of cholesterol in yolk, strengthen the ability of extra large blue laying hen body's immunity and resisting stress, have certain health-care effect to the blue laying hen in sea.Especially with biological compound premixing agent effect for the best.
Table 2 different additive is on the impact of performance in layers
Note: do not have identical lowercase person to represent significant difference (P < 0.05) after same row different rows data, difference extremely significantly (P < 0.01) not have identical capitalization to represent.Lower same.
Table 3 different additive is on the impact of laying hen egg quality
。