CN109287884A

CN109287884A - A kind of compound Chinese herb Tiny ecosystem pulvis and preparation method thereof being exclusively used in egg fowl egg-laying peak

Info

Publication number: CN109287884A
Application number: CN201811153357.8A
Authority: CN
Inventors: 潘慧青; 张炎达; 赵军; 杨荣平; 林贺桂; 赵齐
Original assignee: BRADY FUJIAN PHARMACEUTICAL Co
Current assignee: BRADY FUJIAN PHARMACEUTICAL Co
Priority date: 2018-09-30
Filing date: 2018-09-30
Publication date: 2019-02-01

Abstract

The invention belongs to layer breeding technical fields, disclose a kind of compound Chinese herb Tiny ecosystem pulvis and preparation method thereof for being exclusively used in egg fowl egg-laying peak.It is in terms of 100% by the sum of mass percentage, the compound Chinese herb Tiny ecosystem pulvis is made of each raw material of following mass percentages: bacillus subtilis 5 ~ 10%, enterococcus faecalis 1 ~ 3%, daizeol 0.5 ~ 1%, vitamin E 0.1 ~ 1%, chilli powder 5 ~ 10%, DDGS 15 ~ 20%, hawthorn powder 5 ~ 10%, zeolite powder 5 ~ 15%, Radix Pseudostellariae extract powder 15 ~ 25%, radix pseudostellariae cauline leaf powder 5 ~ 10%, complex enzyme 3 ~ 5%, fishbone dust 0.5 ~ 1.5%, tangerine peel powder 5 ~ 10%, Yellow Croaker Ear-stone 5 ~ 10%, food-grade Pfansteihl 3 ~ 12%, radix pseudostellariae dietary fiber 2 ~ 30%.Compound Chinese herb Tiny ecosystem pulvis of the present invention can extend peak of laying eggs, and improve laying rate, improve eggshell quality, reduce breakage rate.

Description

A kind of compound Chinese herb Tiny ecosystem pulvis being exclusively used in egg fowl egg-laying peak and its system Preparation Method

Technical field

The invention belongs to layer breeding technical fields, and in particular to a kind of compound medium-height grass for being exclusively used in egg fowl egg-laying peak Medicine Tiny ecosystem pulvis and preparation method thereof.

Background technique

Laying hen refers to that raising gets up special raw egg to supply the chicken of egg only, and with meat chicken difference, people raise the master of laying hen Wanting project is to improve egg quality and keep or improve egg production, and not improve chicken meat quality.Egg is the master for raising laying hen Want revenue source.

With the development of scale intensive laying hen production, in actual production many factors can all induce chicken morbidity, Laying rate decline or even death, such as cause the various stress factors of laying hen.Height, height of laying eggs in laying cycle of laying hens laying rate The length of peak phase, eggshell quality greatly affected the economic benefit of breeding layer chicken.Under normal circumstances, domestic Egg Production of Laying Hens peak Phase is about 150 days to 360 days, laying hen in egg laying peak period is more be difficult to support to reach 8 months and its more than, and the peak period later period is past The problems such as toward with shell thickness reduction, rate of broken eggs increase, hen weight loss, excrement sense organ difference and death and culling rate increase, these Have become the pain spot of domestic egg poultry breeding industry.

Due to applying antibiotic that there is apparent economic benefit, in addition current high density large-scale cultivation situation in cultivation Lower animal health condition is poor, and aquaculture is caused more to rely on antibiotic application.In aquaculture industry of China, practitioner is commonly plain In the lower situation of matter, the volume that transfinites, the abuse of antibiotics phenomenon of super kind are generally existing, and antibiotic residue in cultured product is caused to refer to Mark is higher, seriously threatens food safety.Nonreactive cultivation is the abbreviation of antibiotic-free cultivation, refers to and does not have to any type in breeding process Antibiotic, refer in particular to people's antibiotic and beast birds antibiotic need not.

Therefore, the nonreactive feed addictive for developing a kind of egg-laying peak laying hen is just particularly important.

Summary of the invention

In view of the above-mentioned defects in the prior art with deficiency, egg fowl is exclusively used in the purpose of the present invention is to provide one kind and is laid eggs height Compound Chinese herb Tiny ecosystem pulvis of peak phase and preparation method thereof.

To achieve the above object, the technical solution adopted by the present invention is as follows:

A kind of compound Chinese herb Tiny ecosystem pulvis being exclusively used in egg fowl egg-laying peak, with the sum of mass percentage for 100% Meter, the compound Chinese herb Tiny ecosystem pulvis are made of each raw material of following mass percentages: bacillus subtilis 5 ~ 10%, Enterococcus faecalis 1 ~ 3%, daizeol (chemical name 4,7- dihydroxy isoflavone) 0.5 ~ 1%, vitamin E 0.1 ~ 1%, chilli powder 5 ~ 10%, DDGS(corn wine waste matter) 15 ~ 20%, hawthorn powder 5 ~ 10%, zeolite powder 5 ~ 15%, Radix Pseudostellariae extract powder 15 ~ 25%, crown prince Join cauline leaf powder 5 ~ 10%, complex enzyme 3 ~ 5%, fishbone dust 0.5 ~ 1.5%, tangerine peel powder 5 ~ 10%, Yellow Croaker Ear-stone 5 ~ 10%, food-grade Pfansteihl 3 ~ 6%, radix pseudostellariae dietary fiber 2 ~ 30%；

Viable count >=1.0 × 10 of the bacillus subtilis⁸CFU/g, viable count >=1.0 × 10 of enterococcus faecalis⁷ CFU/ g；The potency of the vitamin E >=1000 IU/g.

The complex enzyme is glucose oxidase, alpha-amylase, papain and lipase grape glycosyloxy in mass ratio Change enzyme: alpha-amylase: papain: lipase=1: (1 ~ 3): (1 ~ 2): the mixture of 1 composition, the enzyme of glucose oxidase Vigor is 8000-10000U/g, and the enzyme activity of alpha-amylase enzyme is 8000-10000U/g, and the enzyme activity of papain is 50000-100000 U/g, the enzyme activity of lipase are 50000-100000 U/g.

Preferably, the Radix Pseudostellariae extract powder and radix pseudostellariae dietary fiber prepare as follows:

S1, radix pseudostellariae pretreatment: by radix pseudostellariae successively after three cleaning, baking and shearing processes；

S2, double sections of Steam explosion treatments: using the radix pseudostellariae after shearing as blasting substrates, the double section Steam explosion treatments of progress, that is, successive By Steam explosion treatment I and Steam explosion treatment II, double sections of Steam explosion treatment interval times are 2 ~ 5min；At steam blasting Manage the condition of I are as follows: explosion chamber coefficient is 0.8 ~ 0.9, and explosion steam pressure handles the time up to 0.6 ~ 0.8MPa, explosion pressure maintaining For 5 ~ 10min, blasting time is not higher than 0.00875s；The condition of Steam explosion treatment II are as follows: quick-fried on the same stage with Steam explosion treatment I Broken equipment is with cavity explosion treatment, and for explosion steam pressure up to 1.5 ~ 2.5MPa, it is 10 ~ 30s that explosion pressure maintaining, which handles the time, when explosion Between be not higher than 0.00875s；

S3, hot water extract: being dosed in hot water extractor after the radix pseudostellariae after explosion treatment is collected, hot water amount and crown prince in tank The mass values of ginseng are 15: 1 ~ 20: 1, extract 90 ~ 100 DEG C of hot water temperature, 2 ~ 3h of extraction time, are extracted 1 time；Phase before extraction At the uniform velocity stirred, it is to be extracted to stop stirring when carrying out to 3/5 extraction time；

S4, extracting solution CO₂Processing: after extracting to hot water, obtained extracting solution being cooled to room temperature, and then moves to cleaning not It becomes rusty in steel container, sterile CO is then passed through into extracting solution after cooling₂Gas, CO₂Flow velocity is 10 ~ 15m³/ min, is passed through CO₂ 5 ~ 15min of gas time；

S5, three-stage filtration: extracting solution CO₂Pass through grade one filter, secondary filter and the three-level of tandem compound after processing respectively Filter realizes filtration operation；Wherein, grade one filter and secondary filter are conventional cartridge filter, grade one filter Filter core aperture with secondary filter is respectively 4.5 ~ 5 μm, 0.2 ~ 0.25 μm；Tertiary filter is hollow fiber filter, in The molecular cut off of hollow fiber is 10000 ~ 30000 dalton；

S6, detoxification: the radix pseudostellariae extracting solution after three-stage filtration is successively passed through into D301 resin packed column and NKAII resin is filled Column, extracting solution are 0.15 ~ 0.2MPa into column pressure, finally collect extracting solution；It then, is to calculate basis, solid-liquid with extracting solution Than based on mass volume ratio g/mL, pulvis is made to get Radix Pseudostellariae extract powder in the cyclodextrin powder of addition 3 ~ 5%, spray drying； Wherein, spray drying treatment condition: 180 ~ 200 DEG C of inlet air temperature, 90 ~ 95 DEG C of leaving air temp, 50 ~ 70 DEG C of feed liquid temperature, flow velocity 20~100mL/min；

S7, the radix pseudostellariae dregs of a decoction after three-stage filtration are used to prepare radix pseudostellariae dietary fiber, process are as follows:

(1), water process: 50 ~ 55 DEG C of hot water and the radix pseudostellariae dregs of a decoction are put into reactor tank, and solid, liquid mass ratio is 1: 5 ~ 1: 8, Adjusting pH is 5.0 ~ 5.2, and mixed liquor stirs 6 ~ 8h；

(2), decomposing protein: pH of mixed obtained by (1) is adjusted to 6.5 ~ 7.0, neutral proteinase is added, temperature maintains 40 ~ 50 DEG C, keep 2 ~ 3h；Wherein, in terms of the radix pseudostellariae dregs of a decoction, the additive amount of neutral proteinase is 150 ~ 200U/g；

(3), starch-splitting: reacting liquid temperature obtained by (2) is increased to 65 ~ 70 DEG C, alpha-amylase is added, reacts 1 ~ 2h；Wherein, In terms of radix pseudostellariae dregs of a decoction dry weight, the additive amount of alpha-amylase is 200 ~ 250U/g；

(4), enzyme deactivation: reacting liquid temperature obtained by (3) is increased to 95 ~ 100 DEG C, keeps 0.3 ~ 0.5h；

(5), it rinses: reaction solution obtained by (4) is filtered, filter residue is rinsed with circulating water again；

(6), dry: filter residue obtained by (5) is dried in vacuo in 70 ~ 80 DEG C, 0.06 ~ 0.07MPa；

(7), it crushes and is sieved: being crushed after (6) are dry with pulverizer, cross 80 ~ 100 meshes to get radix pseudostellariae dietary fiber.

Preferably, carry out three-stage filtration using self-control three-level filter system in S5, the three-level filter system include tubular type from Scheming, centrifugation liquid storage tank, power transmitting assembly I, power transmitting assembly II, power transmitting assembly III, grade one filter, second level Filter, tertiary filter and its extraneous subsystem；Power transmitting assembly I is connected in series by kinetic pump I and check-valves I；It is dynamic Power transmission assembly II is connected in series by kinetic pump II and check-valves II；Power transmitting assembly III is by kinetic pump III and check-valves III is connected in series；Grade one filter and secondary filter are conventional cartridge filter；Tertiary filter is self-control filter, Structure includes cylinder, doughnut tread assembly, pedestal；Cylinder body bottom and top are respectively equipped with inlet and liquid outlet, outside cylinder Portion is equipped with interlayer, and the upper and lower ends of interlayer are respectively equipped with interlayer import and interlayer outlet；Doughnut tread assembly includes being evenly equipped with Porous package shell, several doughnuts, these doughnuts merge bunchy and are placed in inside package shell；Pedestal is cylinder Body, cylindrical body upper section be it is hollow be cylindrical in shape, middle and lower sections it is solid, the middle and lower sections of pedestal it is solid equipped with doughnut quantity and its The compatible through-hole of size；Pedestal is located at below liquid outlet, is close to cylinder roof and is firmly connected with it；The upper end of doughnut Opening is simultaneously threaded through in the through-hole of pedestal, and lower end closed is simultaneously fixedly connected with package shell bottom wall；The external world of tertiary filter is attached Category system includes kinetic pump IV, check-valves IV, constant temperature refrigeration water tank, and successively contact check-valves IV, constant temperature chilled water for interlayer outlet Case and kinetic pump IV form a closed cycle system to interlayer import；Tube centrifuge by pipeline successively contact centrifugate storage Tank, kinetic pump I, check-valves I, grade one filter, kinetic pump II, check-valves II, secondary filter, kinetic pump III, check-valves III to tertiary filter inlet；Grade one filter and be respectively 4.5 ~ 5 μm with the filter core aperture of secondary filter, 0.2 ~ 0.22μm；The molecular cut off of doughnut is 10000 ~ 30000 dalton；

Three-stage filtration step are as follows:

(a), start the equipment by S4 treated extracting solution input tube centrifuge inner cavity first and carry out centrifugal work, from The heart 4000 ~ 6000rpm of turn up is centrifuged 20 ~ 30min；The centrifugal clear liquid that will acquire in time after centrifugation is transferred to centrifugate In storage tank, and temperature controlling range is 5 ~ 10 DEG C in the tank；

(b), the cooling centrifugate that upper process obtains is crossed check-valves I by the road and is delivered in grade one filter by starting kinetic pump I, 0.2 ~ 0.6 MPa of input pressure realizes first time coarse filtration；

(c), the by-pass filtration liquid obtained after grade one filter is crossed check-valves II by the road and is delivered to two by starting kinetic pump II In grade filter, 0.2 ~ 0.6 MPa of input pressure realizes second of coarse filtration；

(d), start kinetic pump IV, by temperature obtained in constant temperature refrigeration water tank be 5 ~ 15 DEG C cold water by the road from interlayer into Mouth enters in interlayer, and crosses check-valves IV by the road by interlayer outlet and be recycled in constant temperature refrigeration water tank；To cylinder lumen temperature Degree restarts kinetic pump III and the secondary filtration liquid obtained after secondary filter is crossed check-valves by the road when reaching 5 ~ 15 DEG C III is delivered in tertiary filter by inlet, 0.2 ~ 0.6 MPa of input pressure, and secondary filtration liquid is first from package shell Porous to enter inside package shell, due to the crown_interception of doughnut, the impurity in secondary filtration liquid is blocked in doughnut Outside, and extracting solution then passes through doughnut and enters pedestal from doughnut top opening, then comes out from liquid outlet.

Preferably, S5 further includes that step (e) is instead cleaned: at the end of work to be filtered, can carry out the reverse cleaning of the system Operation, manual 180 ° of integrated regulation power transmitting assemblies I, power transmitting assembly II, power transmitting assembly III direction, that is, it is original Import become exporting, reconnect corresponding pipeline, while starting above-mentioned kinetic pump I, kinetic pump II, kinetic pump III, will clean Purified water enter in cylinder through barrel liquid discharge mouth, then ejected wash water is finally from centrifugation liquid storage tank discharge, each filter pressure in operation Power list index must not be higher than 0.3 MPa, repeat 2 ~ 3 times.

Preferably, be centrifuged between liquid storage tank and kinetic pump I, between check-valves I and grade one filter, grade one filter and dynamic Power pumps between II, between check-valves II and secondary filter, between secondary filter and kinetic pump III, check-valves III and three-level Pipeline between filter takes movable connection method.

Preferably, the movable connection method is clamp connection.

Preferably, tube centrifuge is GF- divergence type.

Preferably, the radix pseudostellariae cauline leaf powder prepares as follows: annual May ~ July, is obtained using artificial Zherong County radix pseudostellariae cauline leaf is washed with water after the harvesting of radix pseudostellariae cauline leaf, dries in the shade after washes clean, crushed after drying in the shade, excessively 80 ~ It is made after 100 meshes.

Preparation method, steps are as follows:

Yellow Croaker Ear-stone is crushed into (60 ~ 80 mesh) first, then adds food-grade Pfansteihl after hawthorn powder and fish brain mountain flour are mixed, then mix Even be placed in sealing container places shady place standing 6-10h, and last 50 ~ 60 DEG C of dryings crush (60 ~ 80 mesh) after dry again, Obtain acidifying mixture；

Accurately weigh respectively bacillus subtilis, enterococcus faecalis, daizeol, vitamin E, radix pseudostellariae dietary fiber, complex enzyme, Above-mentioned 7 kinds of materials are mixed, obtain mixing powder by fishbone dust；

By obtained mixing powder again with chilli powder, DDGS, acidifying mixture, zeolite powder, Radix Pseudostellariae extract powder, radix pseudostellariae stem Ye Fen, tangerine peel powder mix mixing together, and final products are made.

Application method: for whole use in laying hen in egg laying peak period (- 400 days 150 days), every 10-15kg spice 3000kg Feed is fed according to normal feeding method daily, is freely eaten and is drunk water, is managed by laying hen chicken house Routine Management program.

The utility model has the advantages that compound Chinese herb Tiny ecosystem pulvis of the present invention can extend peak of laying eggs, laying rate is improved, improves egg Chitin amount reduces breakage rate.

Detailed description of the invention

Fig. 1: the structural schematic diagram of three-level filter system is made in the present invention by oneself；

Fig. 2: structural schematic diagram when three-level filter system is in anti-cleaning state is made in the present invention by oneself；

Fig. 3: preceding laying hen chicken manure photo is used；

Fig. 4: rear laying hen chicken manure photo is used；

Description of symbols: 1- tube centrifuge, 2- centrifugation liquid storage tank, 31- kinetic pump I, 41- check-valves I, 32- kinetic pump II, 42- check-valves II, 33- kinetic pump III, 43- check-valves III, 34- kinetic pump IV, 44- check-valves IV, 5- grade one filter, 6- Secondary filter, 7- cylinder, 8- inlet, 9- liquid outlet, 10- interlayer, 11- interlayer import, the outlet of 12- interlayer, 13- pedestal, 14- package shell, 15- doughnut, 16- constant temperature refrigeration water tank.

Specific embodiment

To keep the present invention clearer, clear, technical solution of the present invention is further described below.It should manage Solution, the specific embodiments described herein are merely illustrative of the present invention, is not intended to limit the present invention.

In following embodiments and reference examples, the viable count of the bacillus subtilis is 1.0 × 10⁸CFU/g, excrement intestines The viable count of coccus is 1.0 × 10⁷CFU/g, the potency of the vitamin E are 1000 IU/g；The enzyme activity of glucose oxidase Power is 8000 U/g, and the enzyme activity of alpha-amylase enzyme is 8000 U/g, and the enzyme activity of papain is 50000 U/g, fat The enzyme activity of enzyme is 50000 U/g；The mass concentration 80% of food-grade Pfansteihl.

Embodiment 1-3 and reference examples 1-2

A kind of compound Chinese herb Tiny ecosystem pulvis, composition are shown in Table 1 (in terms of mass percentage).

In table 1, the source of the raw material is as follows:

Bacillus subtilis passes through commercially available acquisition；Enterococcus faecalis is applicant in the patent application (Shen that on July 11st, 2017 submits Please number: enterococcus faecalis disclosed in CN201710559483.2) (Enterococcus faecalis) BD-1, and by the bacterial strain It is preserved in China typical culture collection center, address: wuchang, wuhan Luo Jia Shan, preservation date: on July 7th, 2017；Preservation Number: CCTCC NO:M2017416.

The Radix Pseudostellariae extract powder and radix pseudostellariae dietary fiber prepare as follows:

S1, radix pseudostellariae pretreatment: by radix pseudostellariae successively after three cleaning, baking and shearing processes；Wherein, cleaning is by current year The collected radix pseudostellariae summarized carries out clear water cleaning to wash away the soil and other sundries on its surface；Baking refers to is taken out using low temperature Vacuum baking technology, 55 DEG C of baked temperature, baking time 25mim, vacuum pressure 0.04MPa；Shearing refer to by after baking too Son ginseng is sheared using shearing machine equipment, and the radix pseudostellariae length after shearing is 0.8cm；

S2, double sections of Steam explosion treatments: using the radix pseudostellariae after shearing as blasting substrates, the double section Steam explosion treatments of progress, that is, successive By Steam explosion treatment I and Steam explosion treatment II, double sections of Steam explosion treatment interval times are 3min；Steam explosion treatment The condition of I are as follows: explosion chamber coefficient is 0.8, and explosion steam pressure reaches 0.7MPa, and the explosion pressure maintaining processing time is 8min, quick-fried The broken time is not higher than 0.00875s；The condition of Steam explosion treatment II are as follows: with the Steam explosion treatment I same cavity of blasting gear on the same stage Explosion treatment, explosion steam pressure reach 2.0MPa, and explosion pressure maintaining handles the time as 20s, and blasting time is not higher than 0.00875s；

S3, hot water extract: being dosed in hot water extractor after the radix pseudostellariae after explosion treatment is collected, hot water amount and crown prince in tank The mass values of ginseng are 18: 1, extract 95 DEG C of hot water temperature, extraction time 2.5h, are extracted 1 time；The phase is at the uniform velocity stirred before extraction It mixes (revolving speed 100rpm/min), it is to be extracted to stop stirring when carrying out to 3/5 extraction time；

S4, extracting solution CO₂Obtained extracting solution: after extracting to hot water, being cooled to room temperature by processing first with heat exchanger, It then moves in clean rustless steel container, sterile CO is then passed through into extracting solution after cooling₂Gas, CO₂Flow velocity is 13m³/ Min is passed through CO₂Gas time 10min；It is passed through CO₂When gas, CO₂Gas is distributed by pipeline through the gas of rustless steel container bottom Device enters inside extracting solution；

S5, three-stage filtration: extracting solution CO₂After processing, three-stage filtration is carried out using self-control three-level filter system, as shown in Figure 1, should Three-level filter system include GF- divergence type tube centrifuge 1, centrifugation liquid storage tank 2(tank in temperature controlling range be 5~10 DEG C), Power transmitting assembly I, power transmitting assembly II, power transmitting assembly III, grade one filter 5, secondary filter 6, three-stage filtration Device and its extraneous subsystem；

Power transmitting assembly I is connected in series by kinetic pump I 31 and check-valves I 41；Power transmitting assembly II is by kinetic pump II 32 It is connected in series with check-valves II 42；Power transmitting assembly III is connected in series by kinetic pump III 33 and check-valves III 43；

Grade one filter 5 and secondary filter 6 are conventional cartridge filter；Tertiary filter is self-control filter, structure packet Include cylinder 7, doughnut tread assembly, pedestal 13；7 bottom and top of cylinder are respectively equipped with inlet 8 and liquid outlet 9, outside cylinder 7 Portion is equipped with interlayer 10, and the upper and lower ends of interlayer 10 are respectively equipped with interlayer import 11 and interlayer outlet 12；Doughnut tread assembly packet It includes and is evenly equipped with porous package shell 14, quantity 600, the doughnut 15 that molecular cut off is 10000 dalton, in these Hollow fiber 15 merges bunchy and is placed in inside package shell 14；Pedestal 13 be cylindrical body, cylindrical body upper section be it is hollow be cylindrical in shape, under Duan Shixin, the middle and lower sections of pedestal 13 are solid to be equipped with through-hole compatible with 15 quantity of doughnut and its size；Pedestal 13 In 9 lower section of liquid outlet, it is close to 7 roof of cylinder and is firmly connected with it；The upper end of doughnut 15 is open and is threaded through pedestal 13 Through-hole in, lower end closed is simultaneously fixedly connected with 14 bottom wall of package shell；

The extraneous subsystem of tertiary filter includes kinetic pump IV 34, check-valves IV 44, constant temperature refrigeration water tank 16, and interlayer goes out Mouthfuls 12 successively contact check-valves IV 44, constant temperature refrigeration water tank 16 (controllable temperature range are as follows: 5~15 DEG C) and kinetic pump IV 34 to folder Layer import 11 forms a closed cycle system；

Tube centrifuge 1 by pipeline successively contact be centrifuged liquid storage tank 2, kinetic pump I 31, check-valves I 41, grade one filter 5, Kinetic pump II 32, check-valves II 42, secondary filter 6, kinetic pump III 33, check-valves III 43 to tertiary filter into Liquid mouth 8；Wherein, be centrifuged between liquid storage tank 2 and kinetic pump I 31, between check-valves I 41 and grade one filter 5, grade one filter Between 5 and kinetic pump II 32, between check-valves II 42 and secondary filter 6, secondary filter 6 and kinetic pump III 33 it Between, the pipeline between check-valves III 43 and tertiary filter take clamp connection；

By commercially available acquisition, the filter core of grade one filter 5 and secondary filter 6 is equal for aforementioned grade one filter 5 and secondary filter 6 It is respectively 5 and 7,5 He of grade one filter for folding type filter element, grade one filter 5 and with the filter core number of secondary filter 6 The filter core aperture of secondary filter 6 is respectively 5 μm, 0.22 μm；The filter core material of grade one filter 5 and secondary filter 6 is poly- Propylene, frame of filter element (support frame) be rigid plastics, filter core can compression resistance be 0.4 MPa；

Three-stage filtration step are as follows:

(a), in extracting solution input 1 inner cavity of tube centrifuge first obtained S4, start the equipment and carry out centrifugal work, centrifugation Turn up 5000rpm/min is centrifuged 30min；The centrifugal clear liquid that will acquire in time after centrifugation is transferred to centrifugation liquid storage tank 2 It is interior, and temperature controlling range is 10 DEG C in the tank；

(b), the cooling centrifugate that upper process obtains is crossed check-valves I 41 through pipeline and is delivered to level-one mistake by starting kinetic pump I 31 In filter 5,0.2 Mpa of input pressure realizes first time coarse filtration；

(c), it is defeated to be crossed check-valves II 42 through pipeline by starting kinetic pump II 32 for the by-pass filtration liquid obtained after grade one filter 5 It send to secondary filter 6,0.2 Mpa of input pressure, realizes second of coarse filtration；

(d), start kinetic pump IV 34, by temperature obtained in constant temperature refrigeration water tank 16 be 5 DEG C cold water through pipeline from interlayer Import 11 enters in interlayer 10, and crosses check-valves IV 44 through pipeline by interlayer outlet 12 and be recycled in constant temperature refrigeration water tank 16； Restart kinetic pump III 33 when 7 inner cavity temperature of cylinder reaches 5 DEG C to pass through the secondary filtration liquid obtained after secondary filter 6 Pipeline is crossed check-valves III 43 and is delivered in tertiary filter by inlet 8,0.2 Mpa of input pressure, secondary filtration liquid first from Porous on package shell 14 enters inside package shell 14, due to the crown_interception of doughnut 15, in secondary filtration liquid Impurity is blocked in the outside of doughnut 15, and extracting solution then passes through doughnut 15 and enters pedestal from 15 top opening of doughnut 13, then come out from liquid outlet 9；

(e), device is counter cleans: at the end of work to be filtered, can carry out the reverse cleaning operation of the equipment series, i.e., dismantle first Be centrifuged between liquid storage tank 2 and kinetic pump I 31, between check-valves I 41 and grade one filter 5, grade one filter 5 and kinetic pump II Between 32, between check-valves II 42 and secondary filter 6, between secondary filter 6 and kinetic pump III 33, check-valves III The clip on pipeline between 43 and tertiary filter, manual 180 ° of integrated regulation power transmitting assemblies I, power transmitting assembly The direction (original import becomes exporting) of II, power transmitting assembly III, then by the corresponding pipeline of clamp connection, after adjustment State as shown in Fig. 2, starting above-mentioned kinetic pump I 31, kinetic pump II 32, kinetic pump III 33 simultaneously, by clean purifying Water enters in cylinder 7 through 7 liquid outlet 9 of cylinder, then ejected wash water is finally discharged from centrifugation liquid storage tank 2, each filter pressure in operation List index must not be higher than 0.3 MPa, be repeated 2 times；

S6, detoxification: the radix pseudostellariae palpus extracting solution after three-stage filtration is successively passed through into D301 resin packed column and NKAII resin is filled Column, extracting solution are 0.15MPa into column pressure, finally collect extracting solution；It then, is to calculate basis with extracting solution, solid-to-liquid ratio is pressed Mass volume ratio g/mL meter, adds 4% cyclodextrin powder, and pulvis is made to get Radix Pseudostellariae extract powder in spray drying；Wherein, it sprays Mist drying process condition: 200 DEG C of inlet air temperature, 90 DEG C of leaving air temp, 60 DEG C of feed liquid temperature, flow velocity 50mL/min；

(1), water process: 50 DEG C of hot water and the radix pseudostellariae dregs of a decoction are put into reactor tank, and solid, liquid mass ratio is 1: 6, are with concentration The H of 0.5mol/L₂SO₄Adjusting pH is 5.0, and mixed liquor stirs 8h；

(2), decomposing protein: the NaOH solution for being 0.5mol/L with concentration adjusts pH of mixed obtained by (1) to 6.5, is added Neutral proteinase, temperature maintain 45 DEG C, keep 2.5h；Wherein, in terms of the radix pseudostellariae dregs of a decoction, the additive amount of neutral proteinase is 150U/g；

(3), starch-splitting: reacting liquid temperature obtained by (2) is increased to 65 DEG C, alpha-amylase is added, reacts 2h；Wherein, ether Son ginseng dregs of a decoction dry weight meter, the additive amount of alpha-amylase are 200U/g；

(4), enzyme deactivation: reacting liquid temperature obtained by (3) is increased to 95 DEG C, keeps 0.5h；

(5), it rinses: reaction solution obtained by (4) being filtered with nylon cloth, filter residue is rinsed with circulating water again；

(6), dry: filter residue obtained by (5) is dried in vacuo in 75 DEG C, 0.06MPa；

(7), it crushes and is sieved: being crushed, sieved with 100 mesh sieve to get radix pseudostellariae dietary fiber with pulverizer after (6) are dry.

The radix pseudostellariae cauline leaf powder prepares as follows: annual June, obtains Zherong County radix pseudostellariae using artificial Cauline leaf (picks up from the Zherong County planting base radix pseudostellariae GAP), is washed with water after the harvesting of radix pseudostellariae cauline leaf, dries in the shade after washes clean, yin It is crushed after dry, is made after sieving with 100 mesh sieve.

The preparation method of embodiment 1-3 product, steps are as follows:

Yellow Croaker Ear-stone is crushed to 80 mesh first, then adds food-grade Pfansteihl after hawthorn powder and fish brain mountain flour are mixed, then mix It is placed in sealing container and places shady place standing 8h, last 55 DEG C of dryings are crushed to 80 mesh again after dry, obtain acidification mixing Object；

The preparation method of reference examples 1-2 product, steps are as follows:

By obtained mixing powder again with chilli powder, DDGS, acidifying mixture, zeolite powder, Radix Pseudostellariae extract powder, radix pseudostellariae stem Ye Fen, tangerine peel powder mix mixing together, are eventually adding cornstarch mixing, final products are made.

Clinical effectiveness

It carries out as follows:

(1) drug and reagent

Trial drug: product prepared by embodiment 1,2,3；

Control drug 1: product prepared by reference examples 1；

Control drug 2: product prepared by reference examples 2；

Control drug 3: laying hen treasured " Republic of China Veterinary Pharmacopoeia two " version in 2000.

(2) test method

(1) experimental design: test uses single factor test completely randomized design, selects the healthy Roman powder shell layer chicken 2800 of 23 week old Plumage, weight size is almost the same, is randomly divided into 7 groups, every group of 4 repetitions, each 100 plumage chicken of repetition, time test period is 30 Week, laying hen use cage, and 16 h of illumination is freely eaten and drinks water, is managed by chicken house Routine Management program.

Test group 1: mixing 3000kg feed (basal diet) according to every 1 product of 10kg embodiment, is freely eaten；

Test group 2: mixing 3000kg feed (basal diet) according to every 2 product of 10kg embodiment, is freely eaten；

Test group 3: mixing 3000kg feed (basal diet) according to every 3 product of 10kg embodiment, is freely eaten；

Control group 1: mixing 3000kg feed (basal diet) according to every 1 product of 10kg reference examples, is freely eaten；

Control group 2: mixing 3000kg feed (basal diet) according to every 2 product of 10kg reference examples, is freely eaten；

Control group 3: every 500g laying hen treasured is mixed 25kg feed (basal diet), is freely eaten；

Blank control group: being only supplied basal diet, is freely eaten.

Basal diet formula and its nutritional ingredient are shown in Table 2.Feeding management uses 3 layers of cage, other management are according to extensive Laying hen production management system carries out.

(2) testing index: laying rate, average egg weight, egg production, feedstuff-egg ratio, feed consumption rate, extremely naughty chicken number.

(3) test result

As seen from Table 3: the effect of test group 1-3 and control group 1-3 are superior to blank control group, but the effect of test group 1-3 is excellent It is especially best with the effect of test group 3 in control group 1-3, illustrate that efficiency of feed utilization can be improved in feed addictive of the present invention, prolongs Long peak of laying eggs, improves laying rate, improves eggshell quality, reduces breakage rate.

In addition, the laying hen chicken manure before and after 3 use of test group is shown in Fig. 3 and Fig. 4 respectively, it is known that: it is obviously dried using rear excrement Molding, while being significantly reduced using rear excrement ammonia odor, illustrate that feed addictive of the present invention can significantly improve enteron aisle.

Claims

1. a kind of compound Chinese herb Tiny ecosystem pulvis for being exclusively used in egg fowl egg-laying peak, which is characterized in that contained with quality percentage The sum of amount is 100% meter, and the compound Chinese herb Tiny ecosystem pulvis is made of each raw material of following mass percentages: withered grass bud Spore bacillus 5 ~ 10%, enterococcus faecalis 1 ~ 3%, daizeol 0.5 ~ 1%, vitamin E 0.1 ~ 1%, chilli powder 5 ~ 10%, DDGS 15 ~ 20%, hawthorn powder 5 ~ 10%, zeolite powder 5 ~ 15%, Radix Pseudostellariae extract powder 15 ~ 25%, radix pseudostellariae cauline leaf powder 5 ~ 10%, complex enzyme 3 ~ 5%, fishbone dust 0.5 ~ 1.5%, tangerine peel powder 5 ~ 10%, Yellow Croaker Ear-stone 5 ~ 10%, food-grade Pfansteihl 3 ~ 6%, radix pseudostellariae dietary fiber 2 ~ 30%；

Viable count >=1.0 × 10 of the bacillus subtilis⁸CFU/g, viable count >=1.0 × 10 of enterococcus faecalis⁷CFU/g； The potency of the vitamin E >=1000 IU/g.

The complex enzyme is glucose oxidase, alpha-amylase, papain and lipase glucose oxidase in mass ratio: Alpha-amylase: papain: lipase=1: (1 ~ 3): (1 ~ 2): the mixture of 1 composition, the enzyme activity of glucose oxidase are 8000-10000U/g, the enzyme activity of alpha-amylase enzyme are 8000-10000U/g, and the enzyme activity of papain is 50000- 100000 U/g, the enzyme activity of lipase are 50000-100000 U/g.

2. being exclusively used in the compound Chinese herb Tiny ecosystem pulvis of egg fowl egg-laying peak as described in claim 1, which is characterized in that The Radix Pseudostellariae extract powder and radix pseudostellariae dietary fiber prepare as follows:

S5, three-stage filtration: extracting solution CO₂Pass through grade one filter, secondary filter and the three-level mistake of tandem compound after processing respectively Filter realizes filtration operation；Wherein, grade one filter and secondary filter are conventional cartridge filter, grade one filter and The filter core aperture of secondary filter is respectively 4.5 ~ 5 μm, 0.2 ~ 0.25 μm；Tertiary filter is hollow fiber filter, hollow The molecular cut off of fiber is 10000 ~ 30000 dalton；

(7), it crushes and is sieved: crushing, cross 80 ~ 100 meshes to get radix pseudostellariae dietary fiber.

3. being exclusively used in the compound Chinese herb Tiny ecosystem pulvis of egg fowl egg-laying peak as claimed in claim 3, it is characterised in that: In S5 using self-control three-level filter system carry out three-stage filtration, the three-level filter system include tube centrifuge, centrifugation liquid storage tank, Power transmitting assembly I, power transmitting assembly II, power transmitting assembly III, grade one filter, secondary filter, tertiary filter And its extraneous subsystem；Power transmitting assembly I is connected in series by kinetic pump I and check-valves I；Power transmitting assembly II is by power Pump II and check-valves II is connected in series；Power transmitting assembly III is connected in series by kinetic pump III and check-valves III；By-pass filtration Device and secondary filter are conventional cartridge filter；Tertiary filter is self-control filter, and structure includes cylinder, doughnut Tread assembly, pedestal；Cylinder body bottom and top are respectively equipped with inlet and liquid outlet, are equipped with interlayer outside cylinder, interlayer up and down Both ends are respectively equipped with interlayer import and interlayer outlet；Doughnut tread assembly includes being evenly equipped with porous package shell, several Doughnut, these doughnuts merge bunchy and are placed in inside package shell；Pedestal is cylindrical body, and cylindrical body upper section, which is hollow, is in Tubular, middle and lower sections are solid, and the middle and lower sections of pedestal are solid to be equipped with through-hole compatible with doughnut quantity and its size；Pedestal Below liquid outlet, it is close to cylinder roof and is firmly connected with it；The upper end of doughnut is open and is threaded through the logical of pedestal In hole, lower end closed is simultaneously fixedly connected with package shell bottom wall；The extraneous subsystem of tertiary filter includes kinetic pump IV, stops Valve IV, constant temperature refrigeration water tank are returned, successively contact check-valves IV, constant temperature refrigeration water tank and kinetic pump IV to interlayer import for interlayer outlet Form a closed cycle system；Tube centrifuge is successively contacted by pipeline and is centrifuged liquid storage tank, kinetic pump I, check-valves I, level-one Filter, kinetic pump II, check-valves II, secondary filter, kinetic pump III, check-valves III to tertiary filter inlet； Grade one filter and be respectively 4.5 ~ 5 μm, 0.2 ~ 0.22 μm with the filter core aperture of secondary filter；The retention molecule of doughnut Amount is 10000 ~ 30000 dalton；

Three-stage filtration step are as follows:

4. being exclusively used in the compound Chinese herb Tiny ecosystem pulvis of egg fowl egg-laying peak as claimed in claim 3, which is characterized in that S5 further includes that step (e) is instead cleaned: at the end of work to be filtered, can carry out the reverse cleaning operation of the system, manual 180 ° whole Direction, that is, original import of body motivation of adjustment transmission assembly I, power transmitting assembly II, power transmitting assembly III become exporting, Corresponding pipeline is reconnected, while starting above-mentioned kinetic pump I, kinetic pump II, kinetic pump III, by clean purified water through cylinder Liquid outlet enters in cylinder, then finally each filter pressure list index must not be high from centrifugation liquid storage tank discharge, operation for ejected wash water In 0.3 MPa, repeat 2 ~ 3 times.

5. being exclusively used in the compound Chinese herb Tiny ecosystem pulvis of egg fowl egg-laying peak as claimed in claim 3, it is characterised in that: Be centrifuged between liquid storage tank and kinetic pump I, between check-valves I and grade one filter, between grade one filter and kinetic pump II, non-return Pipe between valve II and secondary filter, between secondary filter and kinetic pump III, between check-valves III and tertiary filter Take movable connection method in road.

6. being exclusively used in the compound Chinese herb Tiny ecosystem pulvis of egg fowl egg-laying peak as claimed in claim 5, it is characterised in that: The movable connection method is clamp connection.

7. being exclusively used in the compound Chinese herb Tiny ecosystem pulvis of egg fowl egg-laying peak as claimed in claim 3, it is characterised in that: Tube centrifuge is GF- divergence type.

8. being exclusively used in the compound Chinese herb Tiny ecosystem pulvis of egg fowl egg-laying peak as described in claim 1, which is characterized in that The radix pseudostellariae cauline leaf powder prepares as follows:

Zherong County radix pseudostellariae cauline leaf is obtained using artificial, is washed with water, washs after the harvesting of radix pseudostellariae cauline leaf annual May ~ July It dries in the shade after clean, is crushed after drying in the shade, is made after crossing 80 ~ 100 meshes.

9. it is a kind of claim 1 ~ 8 it is any as described in the compound Chinese herb Tiny ecosystem pulvis for being exclusively used in egg fowl egg-laying peak Preparation method, which is characterized in that steps are as follows:

Yellow Croaker Ear-stone is crushed first, then adds food-grade Pfansteihl after hawthorn powder and fish brain mountain flour are mixed, then mix and be placed on Shady place is placed in sealing container and stands 6-10h, and last 50 ~ 60 DEG C of dryings crush again after dry, obtain acidifying mixture；