CN107821873A - The preparation method of the feed of gut of shrimp function can be improved - Google Patents
The preparation method of the feed of gut of shrimp function can be improved Download PDFInfo
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Abstract
The invention provides a kind of preparation method for the feed for improving gut of shrimp function, comprise the following steps：S1, pressurized tank insulation will be placed in after orange peel, garlic, fennel, stop dysentery grass, sargassum, hawthorn co-grinding, takes out mixture；Mixture is placed in ethanol solution and flowed back, filtrate and filter residue is separated to obtain, filtrate is concentrated to give medicinal extract；S2, Bifidobacterium, lactic acid bacteria, bacillus natto, saccharomyces cerevisiae, molasses, water mixed fermentation obtained into activated spawn；S3, by corn flour, silkworm chrysalis, filter residue, activated spawn mixed fermentation, obtain the first fermentate；Add jujube into the first fermentate, fructus hippophae, clam shell powder continue to ferment, filter, dry the second fermentate；S4, by mixing the second fermentate, medicinal extract, stir, be granulated, drying, produce.The feed of the present invention is that raw material can protect gut of shrimp flatulence with orange peel, garlic, fennel etc., helps digest, anti-inflammatory, can control and prevent the generation of gut of shrimp disease.
Description
Technical field
The present invention relates to degree prawn feed technical field, and in particular to a kind of system for the feed for improving gut of shrimp function
Preparation Method.
Background technology
Prawn culturing is the important industry in China's agricultural economy, and the nutrition of prawn mixed feed is in the whole of prawn culturing
During, occupy important position；Comprehensively high-quality mixed feed is to capture one of key condition of stable high yield for nutrition, is pair
The material base of shrimp healthy aquaculture, it is the important step for influenceing prawn culturing success or failure.In the last few years, with prawn culturing density
Improve, cultivation density continues to increase, the continuous deterioration of breeding water body environment and the degeneration of germplasm resource, causes supporting in prawn
Easily occurs enteritis disease during growing.Juvenile prawn the reasons such as intestinal growth is unsound, causes because its immune and anti-oxidation function is not perfect
Cultivation difficulty is big, and survival rate is low, and very big loss is brought to shrimp culture industry.Therefore need a kind of raising and improve cultivation object
Intestinal health, increase the feed of flesh immunity and disease resistance.
The content of the invention
It is an object of the invention to solve at least the above, and provide the advantages of at least will be described later.
It is an object of the present invention to provide a kind of preparation method for the feed for improving gut of shrimp function, with orange
Skin, garlic, fennel etc. are that raw material can protect gut of shrimp flatulence, are helped digest, anti-inflammatory, can control and prevent gut of shrimp disease
Generation.
In order to realize object of the present invention and further advantage, there is provided a kind of feed for improving gut of shrimp function
Preparation method, comprise the following steps：
S1, by parts by weight be 10~20 parts orange peel, 20~30 parts of garlic, 10~15 parts of fennel, 15~20 parts
Stop dysentery grass, 10~15 parts sargassum, 10~15 parts of Guava Leaf, 10~15 parts of aloe, 5~10 parts of hollow lotus seeds
The pressurized tank that vacuum is -0.05~-0.09Mpa is placed in after leaf, 5~10 parts of dried peppermint leaf, 5~10 parts of hawthorn co-grinding
It is interior, and keep 1~2h, Ran Houzai after temperature is warming up to 60~65 DEG C using heating rate as 0.5~1 DEG C/min in control pressure tank
25~30 DEG C of 0.5~1h of holding are cooled to by 1~2 DEG C/min of rate of temperature fall, then again using heating rate as 1~2 DEG C/min
3~4h is kept after being warming up to 65~70 DEG C, takes out mixture；Then mixture is placed in the quality that parts by weight are 180~200 parts
Flow back 10~15h in the ethanol solution that fraction is 80~90%, separates to obtain filtrate and filter residue, filtrate is concentrated to give into medicinal extract；
S2, by parts by weight be 1~2 part Bifidobacterium, 3~5 parts of lactic acid bacteria, 1~2 part of bacillus natto, 1~2 part
Bacillus subtilis, 2~4 parts of bacillus licheniformis, 1~2 part of enterococcus faecalis, 3~6 parts of saccharomyces cerevisiae, 15~20
The molasses, 50~60 parts of water, 1~2h of fermentation at temperature is 35~37 DEG C of part obtain activated spawn；
S3, the corn flour by parts by weight are 20~30 parts, 15~20 parts of fish meal, 15~20 parts of rapeseed dregs, 15~20
It is 50~60 that the dregs of beans of part, 10~15 parts of silkworm chrysalis, 5~10 parts of filter residue, 10~20 parts of activated spawn, which are mixed after temperature,
Ferment 10~15d at DEG C, obtains the first fermentate；Then jujube, fructus hippophae, collagen, dimension life are added into the first fermentate
Element, clam shell powder, then in temperature be the 2~3d that fermented at 60~70 DEG C, filtering, dry the second fermentate；Wherein, the first hair
Ferment thing, jujube, fructus hippophae, collagen, vitamin, the mass ratio of clam shell powder are 100:5~10:5~10:1~2:0.05
~0.1:10~15；
S4, it is 100 in mass ratio:10~15 the second fermentates of mixing, medicinal extract, stir, are granulated, drying, produce.
Preferably, the preparation method of the described feed for improving gut of shrimp function, mixture is placed in ethanol in S1
In solution flow back before also include feed the mixture into the water that parts by weight are 20~30 parts, temperature is 95~100 DEG C soak 1~
2h, 10~12h is then kept at -20~-25 DEG C, be then that 0.5~1h is kept at 100~120 DEG C then at temperature, then at temperature
Spend to keep 5~10h at -10~-15 DEG C, most flow back after keeping 20~30min to add in ethanol solution at 25~30 DEG C.
Preferably, the preparation method of the described feed for improving gut of shrimp function, the preparation method of clam shell powder
For：After the clam shell that parts by weight are 30~40 parts is crushed, be subsequently placed in the mass concentration that parts by weight are 50~60 parts be 10~
2~3h is soaked at temperature is 30~50 DEG C in 15% phosphoric acid solution, is taken out, is cleaned；Then the clam shell after cleaning is put
20~30min is soaked in the sodium carbonate liquor that the mass concentration that parts by weight are 20~30 parts is 5~8%, is taken out, is cleaned, is done
After being well mixed after dry with the far-infared ceramic powder that parts by weight are 0.5~1 part, calcination, clam shell powder is obtained.
Preferably, the preparation method of the described feed for improving gut of shrimp function, calcination including being prior to temperature
1~2h is calcined at 300~350 DEG C；Then using heating rate as 6~8 DEG C/min be warming up to 550~600 DEG C and keep 1~
1.5h, then be cooled to 350~400 DEG C with 8~10 DEG C/min rate of temperature fall and keep 30~40min, then again with heating rate
700~750 DEG C are warming up to for 10~12 DEG C/min and keeps 0.5~1h, are subsequently cooled to room temperature, are obtained clam shell powder.
Preferably, the preparation method of the described feed for improving gut of shrimp function, also include quality in S4 be
5~10% phagostimulant of the second fermentate quality mixes with the second fermentate, B B-complex, medicinal extract；Wherein described food calling
The preparation of agent comprises the following steps：
Step 1: by parts by weight be 1~2 part anise, 1~2 part of onion, 2~3 parts of garlic, 1~2 part of hawthorn,
2~3 parts of peppermint is added after crushing in the water that parts by weight are 30~40 parts, stirring, and it is 0.1~0.5 part to add parts by weight
1~2h of complex enzyme zymohydrolysis, enzyme deactivation, obtains slurry；Wherein, it is 1 that complex enzyme, which includes mass ratio,:2~3:1~2:1~2:2~3
Lywallzyme, pectase, cellulase, protease and lipase；
Step 2: it is 5~10 parts of maize flour, 5~10 parts of shrimp shell, 2~5 parts of Pizza grass mixed grinding by parts by weight
Cuttlefish powder that Cheng Fenhou and parts by weight are 10~15 parts, 8~10 parts of albumen powder, 0.1~0.5 part of carvacrol, 5~10 parts
Slurry mixes, and is granulated, produces phagostimulant.
Preferably, the preparation method of the described feed for improving gut of shrimp function, the vitamin are included by matter
Amount is than being 3:0.2~0.5:0.5~1:1~2 vitamin A, vitamin B12, vitamin B1, vitamin C composition.
Preferably, the preparation method of the described feed for improving gut of shrimp function, 10~15d processes of fermenting in S3
In, every 2~3d is passed through air and stirred, and the flow for being passed through air is 0.5~1m3/min。
Preferably, the preparation method of the described feed for improving gut of shrimp function, the first fermentate is obtained in S3
Afterwards and 10~20min of irradiation under the infrared lamp that power is 500~1000W is placed on, then carries out second of fermentation.
The present invention comprises at least following beneficial effect：
1st, the raw material such as preparation method, orange peel, garlic, fennel of the feed for improving gut of shrimp function of the invention mixes
Obtained feed can protect gut of shrimp flatulence after being extracted after conjunction crushes and with ethanol, help digest, anti-inflammatory, can control and prevent
The generation of gut of shrimp disease；Wherein, orange peel, fennel have the palatability for improving feed after ethanol extracts and resist micro- life
Thing acts on the grazing rate that can improve prawn, and improves the resistance against diseases of prawn；Stop dysentery grass, peppermint leaf extract have anti-micro- by force
Biology and antioxidation；By the pressurized tank inside holding of each raw material certain vacuum degree before ethanol extraction, in certain vacuum degree
Moisture in lower Chinese herbal medicine can be played with seething with excitement and vaporizing less than under normal boiling point temperature in vaporescence to the cell membrane of Chinese medicine
The effect of broken wall, be advantageous to fully discharge active ingredient in ethanol extraction process is subsequently used, increase drug effect.
2nd, the preparation method of the feed for improving gut of shrimp function of the invention, in activated spawn containing Bifidobacterium,
These probiotics of lactic acid bacteria, bacillus natto, bacillus subtilis, bacillus licheniformis, enterococcus faecalis can increase the immune of prawn
Systemic-function, additionally it is possible to treat enteric flora disturbance, improve intestinal environment, promote enteron aisle unobstructed.
3rd, the preparation method of the feed for improving gut of shrimp function of the invention, by raw materials such as orange peel, garlic, fennels
With immersion, 10~12h of holding at -20~-25 DEG C in the water that temperature is 95~100 DEG C, after raw material contacts hot water, cell membrane
It can expand, there is an effect that part cell reaches broken wall again, and the volume of the aqueous water of the more same quality of volume after water freezing
Increase, moisture build-up ice in the short time trichite life volumetric expansion while, also quickly can fully burst the thin of plant medicinal material
Cell wall, reach more effective shell-broken effect, so as to fully discharge the active ingredient of each raw material, substantially increase drug effect.
4th, the preparation method of the feed for improving gut of shrimp function of the invention, in addition to clam shell, by clam shell powder
First soaked after broken and with phosphoric acid solution and then with after soaking in sodium carbonate solution, and hole can be obtained after repeatedly segmentation is calcined
The porous material that shape is evenly distributed, fine and close so that Nutrient distribution in feed is slowly discharged in hole, extends feeding
The term of validity of material, while cavernous clam shell powder can be with the heavy metal in adsorbed water body, plankton, in a variety of manners
Existing inorganic and organic nitrogen, play the effect of water purification；Far-infared ceramic powder, the far infrared pottery of addition are added in feed simultaneously
Porcelain powder promotes porous formation in clam shell calcination, and makes hole fine and close, while far-infared ceramic powder can kill bacterium in water,
Accelerate moisture movement increase oxygen in water, increase water quality.
5., the preparation method of the feed for improving gut of shrimp function of the present invention, also contain phagostimulant, in phagostimulant
Garlic contains allicin not only with good attractant effect also with bactericidal action；Anise, hawthorn, peppermint have special fragrance
The appetite of prawn can be increased；, can be big containing substantial amounts of amino acid and nucleotides is largely contained in shrimp shell, cuttlefish powder, albumen powder
It is big to stimulate the smell of prawn, the sense of taste it is gathered around feed, accelerate to ingest.
Further advantage, target and the feature of the present invention embodies part by following explanation, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Embodiment
With reference to embodiment, the present invention is described in further detail, to make those skilled in the art with reference to specification
Word can be implemented according to this.
It should be noted that experimental method described in following embodiments, is conventional method unless otherwise specified, institute
Reagent and material are stated, unless otherwise specified, is commercially obtained.
Embodiment 1
A kind of preparation method for the feed for improving gut of shrimp function, comprises the following steps：
S1, the orange peel by parts by weight are 10 parts, 20 parts of garlic, 10 parts of fennel, 15 parts of stop dysentery grass, 10 parts of horse
Tail algae, 10 parts of Guava Leaf, 10 parts of aloe, 5 parts of hollow L otus cotyledon, 5 parts of dried peppermint leaf, 5 parts of hawthorn co-grinding
It is placed in afterwards in the pressurized tank that vacuum is -0.05Mpa, and temperature heats up by 0.5 DEG C/min of heating rate in control pressure tank
Keep 1h after to 60 DEG C, then again using rate of temperature fall as 1 DEG C/min be cooled to 25 DEG C holding 0.5h, then again using heating rate as
1 DEG C/min keeps 3h after being warming up to 65 DEG C, take out mixture；Then mixture is placed in the mass fraction that parts by weight are 180 parts
For 10h, the filtrate of separation and the filter residue of being flowed back in 80% ethanol solution, filtrate is concentrated to give medicinal extract；
S2, the Bifidobacterium by parts by weight are 1 part, 3 parts of lactic acid bacteria, 1 part of bacillus natto, 1 part of bacillus subtilis
Bacterium, 2 parts of bacillus licheniformis, 1 part of enterococcus faecalis, 3 parts of saccharomyces cerevisiae, 15 parts of molasses, 50 parts of water are in temperature
The 1h that fermented at 35 DEG C obtains activated spawn；
S3, the corn flour by parts by weight are 20 parts, 15 parts of fish meal, 15 parts of rapeseed dregs, 15 parts of dregs of beans, 10 parts of silkworm
Pupa, 5 parts of filter residue, the mixing of 10 parts of activated spawn are the 10d that fermented at 50 DEG C after temperature, obtain the first fermentate；Then to
Jujube, fructus hippophae, collagen, vitamin, clam shell powder are added in one fermentate, then ferment 2d at temperature is 60 DEG C,
Filtering, dry the second fermentate；Wherein, first fermentate, jujube, fructus hippophae, collagen, vitamin, clam shell powder
Mass ratio is 100:5:5:1:0.05:10；
S4, it is 100 in mass ratio:10 the second fermentates of mixing, medicinal extract, stir, are granulated, drying, produce.
The preparation method of the described feed for improving gut of shrimp function, mixture, which is placed in ethanol solution, in S1 flows back
It is preceding also to include feeding the mixture into the water that parts by weight are 20 parts, temperature is 95 DEG C soaking 1h, 10h then is kept at -20 DEG C,
Then it is to keep 0.5h at 100 DEG C then at temperature, is to keep 5h at -10 DEG C then at temperature, most after holding 20min at 25 DEG C again
Add in ethanol solution and flow back.
The preparation method of the described feed for improving gut of shrimp function, the preparation method of clam shell powder are：By weight
After part crushes for 30 parts of clam shell, it is subsequently placed in the phosphoric acid solution that the mass concentration that parts by weight are 50 parts is 10% in temperature
To soak 2h at 30 DEG C, take out, clean；Then it is 5% the clam shell after cleaning to be placed in into the mass concentration that parts by weight are 20 parts
Sodium carbonate liquor in soak 20min, take out, with parts by weight be that 0.5 part of far-infared ceramic powder is well mixed after cleaning, drying
Afterwards, it is calcined, obtains clam shell powder.
The preparation method of the described feed for improving gut of shrimp function, it is to be forged at 300 DEG C that calcination, which is included prior to temperature,
Burn 1h；Then 550 DEG C are warming up to as 6 DEG C/min using heating rate and keep 1h, then 350 are cooled to 8 DEG C/min rate of temperature fall
DEG C and keep 30min, be then warming up to 700 DEG C as 10 DEG C/min using heating rate again and keep 0.5h, be subsequently cooled to room temperature,
Obtain clam shell powder.
The preparation method of the described feed for improving gut of shrimp function, it is the second fermentate also to include quality in S4
5% phagostimulant of quality mixes with the second fermentate, B B-complex, medicinal extract；The preparation of wherein described phagostimulant include with
Lower step：
Step 1: it is 1 part of anise, 1 part of onion, 2 parts of garlic, 1 part of hawthorn, 2 parts of Herba Menthae powder by parts by weight
Added after broken in the water that parts by weight are 30 parts, stirring, and add the complex enzyme zymohydrolysis 1h that parts by weight are 0.1 part, enzyme deactivation, starched
Material；Wherein, it is 1 that complex enzyme, which includes mass ratio,:2:1:1:2 lywallzyme, pectase, cellulase, protease and lipase；
Step 2: by parts by weight be 5 parts maize flour, 5 parts of shrimp shell, 2 parts of Pizza grass mixed grinding Cheng Fenhou with again
The cuttlefish powder, 8 parts of albumen powder, 0.1 part of carvacrol, the mixing of 5 parts of slurry that part is 10 parts are measured, is granulated, produces phagostimulant.
Preferably, the preparation method of the described feed for improving gut of shrimp function, the vitamin are included by matter
Amount is than being 3:0.2:0.5:1 vitamin A, vitamin B12, vitamin B1, vitamin C composition.
The preparation method of the described feed for improving gut of shrimp function, during the 10d that ferments in S3, sky is passed through per 2d
Gas simultaneously stirs, and the flow for being passed through air is 0.5m3/min。
The preparation method of the described feed for improving gut of shrimp function, obtain in S3 after the first fermentate and put
It is that 10~20min is irradiated under 500W infrared lamp in power, then carries out second of fermentation.
Embodiment 2
A kind of preparation method for the feed for improving gut of shrimp function, comprises the following steps：
S1, the orange peel by parts by weight are 15 parts, 25 parts of garlic, 12 parts of fennel, 18 parts of stop dysentery grass, 12 parts of horse
Tail algae, 13 parts of Guava Leaf, 12 parts of aloe, 8 parts of hollow L otus cotyledon, 8 parts of dried peppermint leaf, 8 parts of hawthorn co-grinding
It is placed in afterwards in the pressurized tank that vacuum is -0.08Mpa, and temperature heats up by 0.8 DEG C/min of heating rate in control pressure tank
Keep 1h after to 63 DEG C, then again using rate of temperature fall as 1 DEG C/min be cooled to 28 DEG C holding 0.8h, then again using heating rate as
2 DEG C/min keeps 4h after being warming up to 68 DEG C, take out mixture；Then mixture is placed in the mass fraction that parts by weight are 190 parts
For 12h, the filtrate of separation and the filter residue of being flowed back in 85% ethanol solution, filtrate is concentrated to give medicinal extract；
S2, the Bifidobacterium by parts by weight are 2 parts, 4 parts of lactic acid bacteria, 2 parts of bacillus natto, 2 parts of bacillus subtilis
Bacterium, 3 parts of bacillus licheniformis, 2 parts of enterococcus faecalis, 4 parts of saccharomyces cerevisiae, 18 parts of molasses, 55 parts of water are in temperature
The 2h that fermented at 36 DEG C obtains activated spawn；
S3, the corn flour by parts by weight are 25 parts, 18 parts of fish meal, 18 parts of rapeseed dregs, 18 parts of dregs of beans, 12 parts of silkworm
Pupa, 8 parts of filter residue, the mixing of 15 parts of activated spawn are the 12d that fermented at 55 DEG C after temperature, obtain the first fermentate；Then to
Jujube, fructus hippophae, collagen, vitamin, clam shell powder are added in one fermentate, then ferment 3d at temperature is 65 DEG C,
Filtering, dry the second fermentate；Wherein, first fermentate, jujube, fructus hippophae, collagen, vitamin, clam shell powder
Mass ratio is 100:8:8:2:0.08:13；
S4, it is 100 in mass ratio:12 the second fermentates of mixing, medicinal extract, stir, are granulated, drying, produce.
The preparation method of the described feed for improving gut of shrimp function, mixture, which is placed in ethanol solution, in S1 flows back
It is preceding also to include feeding the mixture into the water that parts by weight are 25 parts, temperature is 98 DEG C soaking 2h, 11h then is kept at -23 DEG C,
Then it is to keep 1h at 110 DEG C then at temperature, is to keep 8h at -13 DEG C then at temperature, most adds again after holding 25min at 28 DEG C
Enter in ethanol solution and flow back.
The preparation method of the described feed for improving gut of shrimp function, the preparation method of clam shell powder are：By weight
After part crushes for 35 parts of clam shell, it is subsequently placed in the phosphoric acid solution that the mass concentration that parts by weight are 55 parts is 12% in temperature
To soak 3h at 40 DEG C, take out, clean；Then it is 6% the clam shell after cleaning to be placed in into the mass concentration that parts by weight are 25 parts
Sodium carbonate liquor in soak 25min, take out, with parts by weight be that 0.8 part of far-infared ceramic powder is well mixed after cleaning, drying
Afterwards, it is calcined, obtains clam shell powder.
The preparation method of the described feed for improving gut of shrimp function, it is to be forged at 330 DEG C that calcination, which is included prior to temperature,
Burn 2h；Then 580 DEG C are warming up to as 7 DEG C/min using heating rate and keep 1.5h, then be cooled to 9 DEG C/min rate of temperature fall
380 DEG C and 35min is kept, be then warming up to 730 DEG C as 11 DEG C/min using heating rate again and keep 0.8h, be subsequently cooled to room
Temperature, obtain clam shell powder.
The preparation method of the described feed for improving gut of shrimp function, it is the second fermentate also to include quality in S4
8% phagostimulant of quality mixes with the second fermentate, B B-complex, medicinal extract；The preparation of wherein described phagostimulant include with
Lower step：
Step 1: it is 2 parts of anise, 2 parts of onion, 3 parts of garlic, 2 parts of hawthorn, 2 parts of Herba Menthae powder by parts by weight
Added after broken in the water that parts by weight are 35 parts, stirring, and add the complex enzyme zymohydrolysis 2h that parts by weight are 0.3 part, enzyme deactivation, starched
Material；Wherein, it is 1 that complex enzyme, which includes mass ratio,:3:1:2:3 lywallzyme, pectase, cellulase, protease and lipase；
Step 2: by parts by weight be 8 parts maize flour, 8 parts of shrimp shell, 4 parts of Pizza grass mixed grinding Cheng Fenhou with again
The cuttlefish powder, 9 parts of albumen powder, 0.3 part of carvacrol, the mixing of 8 parts of slurry that part is 13 parts are measured, is granulated, produces phagostimulant.
The preparation method of the described feed for improving gut of shrimp function, it is 3 that the vitamin, which is included by mass ratio,:
0.3:0.8:2 vitamin A, vitamin B12, vitamin B1, vitamin C composition.
The preparation method of the described feed for improving gut of shrimp function, during the 13d that ferments in S3, sky is passed through per 3d
Gas simultaneously stirs, and the flow for being passed through air is 0.8m3/min。
The preparation method of the described feed for improving gut of shrimp function, obtain in S3 after the first fermentate and put
It is to irradiate 15min under 800W infrared lamp in power, then carries out second of fermentation.
Embodiment 3
A kind of preparation method for the feed for improving gut of shrimp function, comprises the following steps：
S1, the orange peel by parts by weight are 20 parts, 30 parts of garlic, 15 parts of fennel, 20 parts of stop dysentery grass, 15 parts of horse
Tail algae, 15 parts of Guava Leaf, 15 parts of aloe, 10 parts of hollow L otus cotyledon, 10 parts of dried peppermint leaf, 10 parts of hawthorn mixed powder
It is placed in after broken in the pressurized tank that vacuum is -0.09Mpa, and temperature heats up by 1 DEG C/min of heating rate in control pressure tank
2h is kept after to 65 DEG C, then 30 DEG C of holding 1h are cooled to by 2 DEG C/min of rate of temperature fall again, then again using heating rate as 2
DEG C/min keeps 4h after being warming up to 70 DEG C, take out mixture；Then mixture is placed in into the mass fraction that parts by weight are 200 parts is
Flow back 15h in 90% ethanol solution, the filtrate of separation and filter residue, filtrate is concentrated to give into medicinal extract；
S2, the Bifidobacterium by parts by weight are 2 parts, 5 parts of lactic acid bacteria, 2 parts of bacillus natto, 2 parts of bacillus subtilis
Bacterium, 4 parts of bacillus licheniformis, 2 parts of enterococcus faecalis, 6 parts of saccharomyces cerevisiae, 20 parts of molasses, 60 parts of water are in temperature
The 2h that fermented at 37 DEG C obtains activated spawn；
S3, the corn flour by parts by weight are 30 parts, 20 parts of fish meal, 20 parts of rapeseed dregs, 20 parts of dregs of beans, 15 parts of silkworm
Pupa, 10 parts of filter residue, the mixing of 20 parts of activated spawn are the 15d that fermented at 60 DEG C after temperature, obtain the first fermentate；Then to
Jujube, fructus hippophae, collagen, vitamin, clam shell powder are added in one fermentate, then ferment 3d at temperature is 70 DEG C,
Filtering, dry the second fermentate；Wherein, first fermentate, jujube, fructus hippophae, collagen, vitamin, clam shell powder
Mass ratio is 100:10:10:2:0.1:15；
S4, it is 100 in mass ratio:15 the second fermentates of mixing, medicinal extract, stir, are granulated, drying, produce.
The preparation method of the described feed for improving gut of shrimp function, mixture, which is placed in ethanol solution, in S1 flows back
It is preceding also to include feeding the mixture into the water that parts by weight are 30 parts, temperature is 100 DEG C soaking 2h, then kept at -25 DEG C
12h, it is then to keep 1h at 120 DEG C then at temperature, is to keep 10h at -15 DEG C then at temperature, is most kept after at 30 DEG C
30min is added in ethanol solution and flowed back.
The preparation method of the described feed for improving gut of shrimp function, the preparation method of clam shell powder are：By weight
After part crushes for 40 parts of clam shell, it is subsequently placed in the phosphoric acid solution that the mass concentration that parts by weight are 60 parts is 15% in temperature
To soak 3h at 50 DEG C, take out, clean；Then it is 8% the clam shell after cleaning to be placed in into the mass concentration that parts by weight are 30 parts
Sodium carbonate liquor in soak 30min, take out, with parts by weight be that 1 part of far-infared ceramic powder is well mixed after cleaning, drying
Afterwards, it is calcined, obtains clam shell powder.
The preparation method of the described feed for improving gut of shrimp function, it is to be forged at 350 DEG C that calcination, which is included prior to temperature,
Burn 2h；Then 600 DEG C are warming up to as 8 DEG C/min using heating rate and keep 1.5h, then be cooled to 10 DEG C/min rate of temperature fall
400 DEG C and 40min is kept, be then warming up to 750 DEG C as 12 DEG C/min using heating rate again and keep 1h, be subsequently cooled to room
Temperature, obtain clam shell powder.
The preparation method of the described feed for improving gut of shrimp function, it is the second fermentate also to include quality in S4
10% phagostimulant of quality mixes with the second fermentate, B B-complex, medicinal extract；The preparation of wherein described phagostimulant include with
Lower step：
Step 1: it is 2 parts of anise, 2 parts of onion, 3 parts of garlic, 2 parts of hawthorn, 3 parts of Herba Menthae powder by parts by weight
Added after broken in the water that parts by weight are 40 parts, stirring, and add the complex enzyme zymohydrolysis 2h that parts by weight are 0.5 part, enzyme deactivation, starched
Material；Wherein, it is 1 that complex enzyme, which includes mass ratio,:3:2:2:3 lywallzyme, pectase, cellulase, protease and lipase；
Step 2: by parts by weight be 10 parts maize flour, 10 parts of shrimp shell, 5 parts of Pizza grass mixed grinding Cheng Fenhou with
Parts by weight are 15 parts of cuttlefish powder, 10 parts of albumen powder, 0.5 part of carvacrol, 10 parts of slurry mix, and are granulated, produce food calling
Agent.
The preparation method of the described feed for improving gut of shrimp function, it is 3 that the vitamin, which is included by mass ratio,:
0.5:1:2 vitamin A, vitamin B12, vitamin B1, vitamin C composition.
The preparation method of the described feed for improving gut of shrimp function, during the 15d that ferments in S3, sky is passed through per 3d
Gas simultaneously stirs, and the flow for being passed through air is 1m3/min。
The preparation method of the described feed for improving gut of shrimp function, obtain in S3 after the first fermentate and put
It is to irradiate 20min under 1000W infrared lamp in power, then carries out second of fermentation.
Comparative example 1
With embodiment 1, difference adds clam shell powder no in S3.
Choose 1000 5 healthy~10g prawn, be randomly divided into 2 groups, using embodiment 1 feed as experimental group,
The feed of Huanghua City Bungay biological products Co., Ltd production as a control group, after feeding 90d, counts enteritis during nursing
State of an illness condition, as a result display has 4 infection enteritis diseases using the feed of embodiment 1, and control group has 13 infection enteritis diseases, explanation
The feed of the present invention can reduce the generation of enteritis disease.
By 400 5 healthy~10g prawn, 2 groups are randomly divided into, using the feed of embodiment 1 as experimental group, Huang
Bungay biological products Co., Ltd of Hua city production feed as a control group, feed 60d after, test every group average growth rate and
Survival number；Then 6 are randomly choosed in each group, is placed in ice pan and dissects rapidly, take anterior intestine to be rushed with 0.86% physiological saline
Wash clean, it is fixed in BouinShi liquid after 24h, carries out paraffin section, measurement and calculating enteron aisle average fluff height, pleat is wide
Degree, pleat height；Take 6 prawns at random respectively in each group, take anterior intestine to smash rear water-bath homogenate to pieces, centrifuging and taking supernatant measure is flat
Equal enteron aisle trypsase, lipase and amylase, as a result as shown in table 1.
As can be seen from Table 1 after the feed of the present invention is fed, the height of naps of gut of shrimp, pleat width, pleat
Control group is highly above, and the trypsase of enteron aisle, lipase and amylase activity are also significantly greater than control group.Illustrate this
The feed of invention can promote intestinal epithelial cell to grow, and strengthen the abilities of digestive and absorption at enteron aisle dominant digestion position, promote intestines
Road digests enzyme secretion, improves gut of shrimp function.
The DIFFERENT FEED of table 1 is to gut of shrimp morphology influence
| Experimental group | Control group | |
| Height of naps (um) | 1.23 | 0.91 |
| Pleat height (um) | 38.36 | 31.28 |
| Pleat width (um) | 27.31 | 24.18 |
| Trypsase (U/mgprot) | 1428.52 | 1038.36 |
| Lipase (U/mgprot) | 22.12 | 18.53 |
| Amylase (U/mgprot) | 179.45 | 136.36 |
| Rate of body weight gain (%) | 232.5 | 206.12 |
| Survival number (only) | 199 | 183 |
Prawn feed prepared by prawn feed and comparative example 1 prepared by embodiment 1, carries out cultivation ratio under the same conditions
Compared with, and the index for cultivating raw water is：DO is 4.18mg/L, and COD 15.93mg/L, TN 1.38mg/L, TP are
0.085mg/L, front and rear change of water quality in breeding process is determined, as a result as shown in table 2；
Table 2 cultivates front and rear change of water quality
The feed of embodiment 1 is in cultured prawn breeding process as can be seen from Table 1, DO increases, COD, TN, TP under
Drop, water quality reach cultivation water quality standard.The feed cultured prawn of comparative example 1, although indices are also superior to raw water, embodiment 1
Feed by comparison, be all higher than the incrementss of control group, COD, TN, TP decline in cultivation early stage and later stage DO incrementss
Amount is all higher than the slippage of control group, therefore the feed of the present invention has good purifying water effect.
Although embodiment of the present invention is disclosed as above, it is not restricted in specification and embodiment listed
With it can be applied to various suitable the field of the invention completely, can be easily for those skilled in the art
Other modification is realized, therefore under the universal limited without departing substantially from claim and equivalency range, it is of the invention and unlimited
In specific details.
Claims (8)
1. a kind of preparation method for the feed for improving gut of shrimp function, it is characterised in that comprise the following steps：
S1, by parts by weight be 10~20 parts orange peel, 20~30 parts of garlic, 10~15 parts of fennel, 15~20 parts only
Dysentery grass, 10~15 parts of sargassum, 10~15 parts of Guava Leaf, 10~15 parts of aloe, 5~10 parts of hollow L otus cotyledon, 5
It is placed in after~10 parts of dried peppermint leaf, 5~10 parts of hawthorn co-grinding in the pressurized tank that vacuum is -0.05~-0.09Mpa,
And 1~2h is kept in control pressure tank after temperature is warming up to 60~65 DEG C using heating rate as 0.5~1 DEG C/min, then again with
Rate of temperature fall is that 1~2 DEG C/min is cooled to 25~30 DEG C of 0.5~1h of holding, then again using heating rate as 1~2 DEG C/min liters
Temperature takes out mixture to 3~4h of holding after 65~70 DEG C；Then mixture is placed in the quality point that parts by weight are 180~200 parts
Number is the 10~15h that flowed back in 80~90% ethanol solution, separates to obtain filtrate and filter residue, filtrate is concentrated to give into medicinal extract；
S2, the Bifidobacterium by parts by weight are 1~2 part, 3~5 parts of lactic acid bacteria, 1~2 part of bacillus natto, 1~2 part withered
Careless bacillus, 2~4 parts of bacillus licheniformis, 1~2 part of enterococcus faecalis, 3~6 parts of saccharomyces cerevisiae, 15~20 parts
Molasses, 50~60 parts of water are that 1~2h of fermentation obtains activated spawn at 35~37 DEG C in temperature；
S3, the corn flour by parts by weight are 20~30 parts, 15~20 parts of fish meal, 15~20 parts of rapeseed dregs, 15~20 parts
Dregs of beans, 10~15 parts of silkworm chrysalis, 5~10 parts of filter residue, the mixing of 10~20 parts of activated spawn are at temperature is 50~60 DEG C
Ferment 10~15d, obtains the first fermentate；Then jujube, fructus hippophae, collagen, vitamin, text are added into the first fermentate
Concha Meretricis Seu Cyclinae powder, then in temperature be the 2~3d that fermented at 60~70 DEG C, filtering, dry the second fermentate；Wherein, the first fermentate,
Jujube, fructus hippophae, collagen, vitamin, the mass ratio of clam shell powder are 100:5~10:5~10:1~2:0.05~0.1:
10~15；
S4, it is 100 in mass ratio:10~15 the second fermentates of mixing, medicinal extract, stir, are granulated, drying, produce.
2. the preparation method of the feed of gut of shrimp function can be improved as claimed in claim 1, it is characterised in that mixed in S1
Thing, which is placed in ethanol solution before backflow, also to be included feeding the mixture into the water that parts by weight are 20~30 parts, temperature is 95~100 DEG C
1~2h of middle immersion, then at -20~-25 DEG C keep 10~12h, then then at temperature be 100~120 DEG C at keep 0.5~
1h, it is that 5~10h is kept at -10~-15 DEG C then at temperature, it is most molten after keeping 20~30min to add ethanol at 25~30 DEG C
Flowed back in liquid.
3. the preparation method of the feed of gut of shrimp function can be improved as claimed in claim 1, it is characterised in that clam shell powder
Preparation method be：After the clam shell that parts by weight are 30~40 parts is crushed, the quality that parts by weight are 50~60 parts is subsequently placed in
In the phosphoric acid solution that concentration is 10~15% in temperature be at 30~50 DEG C immersion 2~3h, take out, clean；Then after cleaning
Clam shell be placed in the sodium carbonate liquor that the mass concentration that parts by weight are 20~30 parts is 5~8% 20~30min of immersion, take
Go out, after being well mixed after cleaning, drying with the far-infared ceramic powder that parts by weight are 0.5~1 part, calcination, obtain clam shell powder.
4. the preparation method of the feed of gut of shrimp function can be improved as claimed in claim 3, it is characterised in that calcination includes
It is that 1~2h is calcined at 300~350 DEG C prior to temperature；Then 550~600 DEG C are warming up to simultaneously by 6~8 DEG C/min of heating rate
1~1.5h is kept, then is cooled to 350~400 DEG C with 8~10 DEG C/min rate of temperature fall and keeps 30~40min, then again with liter
Warm speed is that 10~12 DEG C/min is warming up to 700~750 DEG C and keeps 0.5~1h, is subsequently cooled to room temperature, obtains clam shell powder.
5. the preparation method of the feed of gut of shrimp function can be improved as claimed in claim 1, it is characterised in that also wrapped in S4
Include and mix quality with the second fermentate, B B-complex, medicinal extract for 5~10% phagostimulant of the second fermentate quality；Its
Described in the preparation of phagostimulant comprise the following steps：
Step 1: it is 1~2 part of anise, 1~2 part of onion, 2~3 parts of garlic, 1~2 part of hawthorn, 2~3 by parts by weight
Part peppermint crush after to add parts by weight be stirring in 30~40 parts of water, and it is 0.1~0.5 part compound to add parts by weight
Enzyme digests 1~2h, enzyme deactivation, obtains slurry；Wherein, it is 1 that complex enzyme, which includes mass ratio,:2~3:1~2:1~2:2~3 molten wall
Enzyme, pectase, cellulase, protease and lipase；
Step 2: by maize flour that parts by weight are 5~10 parts, 5~10 parts of shrimp shell, 2~5 parts of Pizza grass mixed grinding into powder
It is afterwards 10~15 parts of cuttlefish powder, 8~10 parts of albumen powder, 0.1~0.5 part of carvacrol, 5~10 parts of slurry with parts by weight
Mixing, it is granulated, produces phagostimulant.
6. the preparation method of the feed of gut of shrimp function can be improved as claimed in claim 1, it is characterised in that the dimension life
It is 3 that element, which is included by mass ratio,:0.2~0.5:0.5~1:1~2 vitamin A, vitamin B12, vitamin B1, vitamin C group
Into.
7. the preparation method of the feed of gut of shrimp function can be improved as claimed in claim 1, it is characterised in that fermented in S3
During 10~15d, every 2~3d is passed through air and stirred, and the flow for being passed through air is 0.5~1m3/min。
8. the preparation method of the feed of gut of shrimp function can be improved as claimed in claim 1, it is characterised in that obtained in S3
After first fermentate and 10~20min of irradiation under the infrared lamp that power is 500~1000W is placed on, then carries out second of hair
Ferment.
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