CN111296659A - Efficient grain-saving immune type fermented compound feed for weaned piglets and preparation method thereof - Google Patents

Efficient grain-saving immune type fermented compound feed for weaned piglets and preparation method thereof Download PDF

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CN111296659A
CN111296659A CN202010243982.2A CN202010243982A CN111296659A CN 111296659 A CN111296659 A CN 111296659A CN 202010243982 A CN202010243982 A CN 202010243982A CN 111296659 A CN111296659 A CN 111296659A
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parts
percent
preparation
mass content
expanded
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谢红兵
刘长忠
刘瑞雪
张英涛
陈国参
姜金庆
崔艳红
王永强
李国旺
苗志国
李勇超
乔英英
申楠
杨树梅
张良慧
皇甫政
常新耀
魏刚才
贺永惠
刘兴友
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Henan Institute of Science and Technology
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Henan Institute of Science and Technology
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/30Feeding-stuffs specially adapted for particular animals for swines
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/60Feeding-stuffs specially adapted for particular animals for weanlings

Abstract

The invention discloses an efficient grain-saving immune fermented compound feed for weaned pigs and a preparation method thereof, and belongs to the technical field of animal nutrition and feed. The invention discloses an efficient grain-saving immune fermented compound feed for weaned pigs, which is prepared by solid-state fermentation of various raw materials. The feed prepared by the invention has the advantages of good digestibility, high utilization efficiency of agricultural and sideline products, high feed safety, low discharge of nitrogen, phosphorus and heavy metals, high immunity of weaned piglets, low diarrhea rate, low morbidity and high production performance.

Description

Efficient grain-saving immune type fermented compound feed for weaned piglets and preparation method thereof
Technical Field
The invention relates to the technical field of animal nutrition and feed, in particular to a weaned piglet efficient grain-saving immune type fermented compound feed and a preparation method thereof.
Background
A weaned piglet is a non-suckling piglet from the stage of weaning to the age of 10 weeks and is mainly characterized in that ① weaning stress is expressed by that liquid breast milk is taken as a main feed, dyspepsia diarrhea is easily generated, ② needs to adapt to a new living environment, a home delivery room is changed into a breeding house and is accompanied with grouping and grouping again, ③ environment is changed, diseases are easily generated, ④ weaned piglets grow and develop quickly, the demand on nutrients and energy is large, but the function of digestive organs is still incomplete, therefore, the improvement of feed digestibility is a key factor for determining the success rate of weaned piglet feeding, and the control of survival rate, diarrhea rate and improvement of production performance are important indexes for determining the feeding benefit of the piglets.
The high-quality feed raw materials of domestic pigs such as corn, bean pulp, fish meal and the like are very short-cut, the bean pulp is mainly derived from imported soybeans, the dependency degree on import reaches more than 75%, the dependency degree on import of the fish meal reaches more than 70%, and the feeding corn accounts for more than 63% of the total corn yield in China, so that the gap is large. In addition, the compound feed for weaned piglets mainly takes corn-soybean meal-fish meal type as the main feed in China, and is under the market challenge and impact under the conditions that the prices of corn, soybean meal and fish meal are high and the profit margin of feed products is reduced. Agricultural and sideline products such as cyperus bean pulp, peanut vine and the like in China are hardly used for pig feed, so that resource waste and environmental pollution are caused, and the agricultural and sideline products such as bean curd residue, leather powder, brown rice, peanut kernel meal and the like are relatively more but are not scientifically and fully utilized.
Therefore, the problem to be solved by technical personnel in the field is to provide the high-efficiency grain-saving immune fermented compound feed for the weaned pigs and the preparation method thereof.
Disclosure of Invention
Aiming at the development characteristics and the digestion physiological characteristics of weaned pigs, the invention designs the efficient grain-saving immune fermented compound feed which can fully utilize agricultural and sideline products such as cyperus bean pulp, peanut vine powder, bean curd residue and the like, improve the digestibility of the feed, reduce the diarrhea rate and the morbidity, has no chemical synthetic drugs and feed antibiotics and improve the production performance of the weaned pigs and has no chemical synthetic drugs or feed antibiotics.
In order to achieve the purpose, the invention adopts the following technical scheme:
an efficient grain-saving immune fermented compound feed for weaned piglets is prepared by solid-state fermentation of the following raw materials in parts by weight: 25.00 to 26.00 parts of water, 7.94 to 9.33 parts of expanded cyperus bean pulp, 1.94 to 4.42 parts of steam-exploded peanut meal, 1.29 to 2.68 parts of steam-exploded bean curd residue, 2.00 to 3.00 parts of expanded leather powder, 7.11 to 10.41 parts of expanded brown rice powder, 4.00 to 5.00 parts of expanded peanut kernel meal, 2.50 to 3.50 parts of beer yeast, 2.00 to 3.00 parts of fish meal, 0.64 to 0.25 part of expanded corn 42.38, 1.21 to 4.39 parts of expanded full-fat soybean, 2.00 to 4.00 parts of whey powder, 5.09 to 5.74 parts of fish oil, 0.29 to 0.33 part of calcium carbonate, 0.64 to 0.76 part of calcium hydrophosphate, 0.30 part of salt, 0.25 to 0.35 part of sodium bicarbonate, 0.15 part of microelement compound premix, 0.15 part of vitamin compound premix, 0.81 to 0.81 part of L-0.85 part of L-0.09 to 0.12.12 parts of L-L tryptophan, 0.06 part of L-0.09 to 0.35 part of L-0.06 part of L-L threonine, 0.41-0.45 part of glucose, 0.45 part of zymocyte preparation, 0.02-0.04 part of phytase, 0.02-0.04 part of complex enzyme preparation, 0.10 part of lactic acid, 0.20-0.40 part of plant extract, 0.02-0.04 part of antibacterial peptide preparation, 0.30-0.50 part of oligomeric chitosan, 0.45-0.55 part of peony, 0.40-0.45 part of fructus evodiae rhizoma coptidis, 0.40-0.45 part of cinnamon, 0.20-0.25 part of spina date seed, 0.20-0.25 part of paniculate swallowwort root, 0.20-0.25 part of three-paired nodes, 0.20-0.25 part of phyllanthus urinaria and 0.08-0.12 part of honey-fried licorice root.
The preparation method of the steam-exploded peanut vine powder comprises the steps of carrying out steam explosion and expansion on the peanut vine powder under the conditions of steam explosion pressure of 2.4MPa, liquid-solid ratio of 3:2, vitamin pressing time of 10min and material granularity of 50 meshes; the preparation method of steam-exploded bean curd residue comprises steam-exploding bean curd residue under steam explosion pressure of 2.4MPa, liquid-solid ratio of 3:2, pressure maintaining time of 10min, and material granularity of 50 mesh.
The specific preparation method of the fructus evodiae processed coptis comprises the following steps: soaking fructus evodiae (100g) in 8 times of water for 20 min, decocting for 45 min, filtering, decocting the residue with 6 times of water for 30 min, filtering, mixing filtrates, concentrating to a certain volume (100mL) to obtain decoction containing 1g fructus evodiae per mL, and cooling; uniformly spraying the evodia rutaecarpa concentrated solution on the surface of coptis chinensis with the mass being 9 times (900g), drying for 30 minutes at the temperature of 100 ℃, and crushing the finished product obtained by processing into fine powder to obtain the evodia rutaecarpa rhizoma coptidis.
Furthermore, the zymocyte preparation is prepared by adding any two of bifidobacterium bifidum, candida utilis, enterococcus faecalis and candida albicans on the basis of lactobacillus plantarum, bacillus, saccharomyces cerevisiae and trichoderma viride, has no specific requirements on the number of live bacteria of each bacterium, and can be prepared when the total number of the live bacteria reaches more than 120 hundred million/g.
Further, the mass content of the crude protein of the puffed cyperus esculentus meal is more than 15.40%; the mass content of crude protein of the steam-exploded peanut vine powder is more than 7.80 percent; the mass content of the crude protein of the steam-exploded bean curd residue is more than 22.50 percent; the mass content of the crude protein of the expanded leather powder is above 74.50%; the mass content of crude protein of the puffed brown rice powder is more than 8.80 percent; the mass content of the crude protein of the puffed peanut kernel meal is above 47.50%; the mass content of the crude protein of the beer yeast is above 74.50%; the mass content of the crude protein of the fish meal is more than 60.20 percent; the mass content of the crude protein of the puffed corn is more than 8.70 percent; the mass content of the crude protein of the puffed full-fat soybean is more than 35.50 percent; the crude protein mass content of the whey powder is more than 22.50 percent; the total mass content of eicosapentaenoic acid and docosahexaenoic acid of the fish oil is more than 20.00 percent; the calcium content of the calcium carbonate is more than 38.00 percent; the phosphorus content of the calcium hydrophosphate is more than 18.00 percent by mass; the mass content of L-lysine in the L-lysine is 70.0 percent; the DL-methionine content of DL-methionine is 99.0%; the mass content of L-tryptophan in the L-tryptophan is 99.0 percent; the L-threonine mass content of the L-threonine is 99.0%; the glucose mass content of the glucose is more than 98.0 percent; the phytase activity of the phytase is more than 2000U/g; the lactic acid mass content of the lactic acid is more than 98.0 percent; the oligochitosan of the oligochitosan has the mass content of more than 99.0 percent.
The compound enzyme preparation is further prepared by mixing xylanase, protease, amylase, β -mannase, cellulase and β -glucanase, wherein the xylanase activity in the compound enzyme preparation is over 12000U/g, the protease activity is over 5200U/g, the amylase activity is over 180000U/g, the β -mannase activity is over 450U/g, the cellulase activity is over 9000U/g and the β -glucanase activity is over 22000U/g.
Further, the plant extract is a uniform mixture consisting of 50% of honeysuckle branch and leaf extract, 20% of gynostemma pentaphylla extract, 20% of momordica grosvenori extract and 10% of rabdosia rubescens extract.
The preparation method of the honeysuckle branch and leaf extract comprises the following steps: taking clean honeysuckle branches and leaves, adding 70% ethanol solution according to the liquid-material ratio of 14:1, extracting for 2 times, extracting for 1.5h each time, preparing an extracting solution, filtering, recovering ethanol from filtrate, concentrating to a proper amount, and drying in vacuum to obtain powder, namely the honeysuckle branches and leaves ethanol extract.
Further, a preparation method of the high-efficiency grain-saving immune fermented compound feed for weaned pigs comprises the following specific steps:
(1) weighing specified parts by weight of puffed cyperus esculentus seed meal, steam-exploded peanut vine powder, steam-exploded bean curd residue, puffed leather powder, puffed brown rice powder, beer yeast, fish meal, puffed corn, puffed full-fat soybean, peony, fructus evodiae-processed coptis chinensis, cinnamon, spina date seed, paniculate swallowwort root, three-paired knots, phyllanthus urinaria and honey-fried licorice root, and primarily mixing; then, crushing for the first time by a crusher and sieving by a 12-mesh sieve, crushing for the second time by an ultrafine crusher and sieving by a 80-mesh sieve to prepare an ultrafine crushing mixture;
(2) weighing whey powder, calcium carbonate, calcium hydrophosphate, salt, sodium bicarbonate, a microelement compound premix, a vitamin compound premix, L-lysine, DL-methionine, L-tryptophan, L-threonine, phytase, a compound enzyme preparation, a plant extract, an antibacterial peptide preparation and oligochitosan in specified weight parts, stirring and mixing uniformly to prepare a small material premix;
(3) weighing water, glucose, lactic acid, a zymocyte preparation and crushed 60-mesh puffed peanut kernel meal in specified weight parts, pouring the mixture into a clean container, uniformly stirring, carrying out constant-temperature shaking culture at 37 ℃ for 3.5 hours, and activating the zymocyte preparation to prepare a strain activation solution;
(4) weighing fish oil in a specified weight part, and uniformly stirring and mixing the fish oil with the superfine grinding mixture prepared in the step (1), the small material premix prepared in the step (2) and the strain activation liquid prepared in the step (3) to prepare a fermented compound feed initial substance;
(5) and (3) putting the initial material of the fermented compound feed prepared in the step (4) into a closed container or a fermentation bag, performing anaerobic fermentation, and standing for 3.0-4.5 days at the temperature of 30-42 ℃ to prepare a finished product of the fermented compound feed.
According to the technical scheme, compared with the prior art, the invention discloses and provides the efficient grain-saving immune fermented compound feed for the weaned pigs and the preparation method thereof, and the efficient grain-saving immune fermented compound feed has the following beneficial effects:
(1) agricultural and sideline products such as cyperus esculentus seed meal, peanut seedlings, bean curd residues, leather powder, brown rice, peanut kernel meal and the like are selected, the nutritional value and anti-nutritional factors of the feed are evaluated, and a foundation is laid for optimizing the parameters of the puffing or steam explosion processing technology of the feed; (2) the puffing process of the cyperus bean pulp, the brown rice, the corn, the full-fat soybean, the peanut kernel pulp and the leather powder is optimized, and the steam explosion process of the bean curd residue and the peanut vine powder is optimized, so that the anti-nutritional factors of the feed are reduced, the natural toxicity of the feed is reduced, the palatability is improved, the digestibility of the feed is improved, and conditions are provided for more sufficient and faster microbial fermentation of the compound feed; (3) evaluating the nutritional value, feeding value and anti-nutritional factors of the feed for the weaned piglets by the raw materials such as the puffed coarse rice powder, the puffed corn, the steam-exploded bean curd residue, the steam-exploded peanut vine powder, the puffed cyperus bean meal, the puffed full-fat soybean, the puffed peanut kernel meal, the puffed leather powder and the like, and laying a foundation for the preliminary design of the feed formula; (4) in order to replace the feed antibiotics, a Chinese herbal medicine feed additive formula and a combination scheme of plant extracts, antibacterial peptide preparations, chitosan oligosaccharide, complex enzyme preparations and the like are selected and optimized; (5) aiming at maintaining intestinal health, enhancing immune function, reducing environmental emission and fully utilizing agricultural and sideline products, taking low heavy metal, no antibiotics and nutrition balance as principles, aiming at the development characteristics and the digestive physiological characteristics of weaned piglets, and primarily designing a formula of a low-protein compound feed based on the nutritional component requirements of piglet and growing-finishing pig compound feed (Chinese feed industry Association group standard, T/CFIAS-2018); (6) optimizing fermentation process parameters of the compound feed, and evaluating the feed nutritive value of the fermented compound feed; (7) optimizing a formula scheme of the fermented compound feed according to the filling and shortage data of the nutrient components of the feed before and after fermentation so as to improve the nutrition and pertinence of the fermented compound feed to weaned piglets; (8) evaluating the influence and action mechanism of the fermented compound feed on the production performance of the weaned piglets.
The weaned pig high-efficiency grain-saving immune fermented compound feed has the advantages that feed components, treatment modes, component proportion, fermentation process parameters and the like have strong pertinence to the feeding effect of the weaned pigs; the prepared feed has the advantages of good digestibility, high utilization efficiency of agricultural and sideline products, high feed safety, low discharge of nitrogen, phosphorus and heavy metals, high immunity of weaned piglets, low diarrhea rate, low morbidity and high production performance.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Puffed corn, puffed whole soybean, puffed cyperus bean pulp, puffed leather powder, puffed coarse rice powder and puffed peanut kernel pulp are all purchased from Shennong puffed feed science and technology Limited in Henan; the fish meal is full-fat fish meal (CP 60.6%) purchased from Qingdao Shengzhikang biotech limited; the whey powder is the American bang Jia whey powder purchased from Shenzhen Fuzhan actual development Limited company; fish oil was purchased from south Jingxi minou Biotech limited; chitosan oligosaccharide was purchased from Qingdao Bozhihui Biotech limited; brewers yeast was purchased from Cangzhou Laisen Biotechnology, Inc.; the microelement compound premix is a special low-additive microelement compound premix for suckling piglets produced by Zhan shoukang feed Limited in New county; the vitamin compound premix is a special vitamin compound premix for the suckling piglets produced by Zhan shoukang feed Limited company in Xinxiang city; the antibacterial peptide preparation is cecropin produced by Beijing Zhongnong Yingtai biotechnology limited; the Gynostemma pentaphyllum extract is purchased from Xianbao Yifeng biotechnology limited; the fructus momordicae extract is purchased from Shaanxi Hunengda Biotech limited company; rabdosia rubescens extract was purchased from Shanxi pannier Biotech limited.
The preparation method of the steam-exploded peanut vine powder comprises the steps of carrying out steam explosion and expansion on the peanut vine powder under the conditions of steam explosion pressure of 2.4MPa, liquid-solid ratio of 3:2, vitamin pressing time of 10min and material granularity of 50 meshes; the preparation method of steam-exploded bean curd residue comprises steam-exploding bean curd residue under steam explosion pressure of 2.4MPa, liquid-solid ratio of 3:2, pressure maintaining time of 10min, and material granularity of 50 mesh.
The specific preparation method of the fructus evodiae processed coptis comprises the following steps: soaking fructus evodiae (100g) in 8 times of water for 20 min, decocting for 45 min, filtering, decocting the residue with 6 times of water for 30 min, filtering, mixing filtrates, concentrating to a certain volume (100mL) to obtain decoction containing 1g fructus evodiae per mL, and cooling; uniformly spraying the evodia rutaecarpa concentrated solution on the surface of coptis chinensis with the mass being 9 times (900g), drying for 30 minutes at the temperature of 100 ℃, and crushing the finished product obtained by processing into fine powder to obtain the evodia rutaecarpa rhizoma coptidis.
Lactobacillus plantarum accession number GIM 1.191; the bacillus has a deposit number of GIM 1.403; the preservation number of the saccharomyces cerevisiae is GIM 2.46; the preservation number of the trichoderma viride is GIM 3.597; the deposit number of the bifidobacterium bifidum is CICC 6071; the preservation number of the candida utilis is GIM 2.176; the preservation number of the enterococcus faecalis is GIM 1.612; the accession number of Candida albicans is GIM 2.194.
The preparation method of the honeysuckle branch and leaf extract comprises the following steps: taking clean honeysuckle branches and leaves, adding 70% ethanol solution according to the liquid-material ratio of 14:1, extracting for 2 times, extracting for 1.5h each time, preparing an extracting solution, filtering, recovering ethanol from filtrate, concentrating to a proper amount, and drying in vacuum to obtain powder, namely the honeysuckle branches and leaves ethanol extract.
Example 1
An efficient grain-saving immune fermented compound feed for weaned piglets is prepared by solid-state fermentation of the following raw materials in parts by weight: 25.00 parts of water, 7.94 parts of expanded cyperus bean pulp, 1.94 parts of steam-exploded peanut vine meal, 1.29 parts of steam-exploded bean curd residue, 3.00 parts of expanded leather powder, 7.11 parts of expanded brown rice powder, 4.00 parts of expanded peanut kernel meal, 3.50 parts of beer yeast, 3.00 parts of fish meal, 50.25 parts of expanded corn, 1.21 parts of expanded full-fat soybean, 4.00 parts of whey powder, 5.09 parts of fish oil, 0.33 part of calcium carbonate, 0.64 part of calcium hydrophosphate, 0.30 part of salt, 0.35 part of sodium bicarbonate, 0.15 part of microelement composite premix, 0.15 part of vitamin composite premix, 0.85 part of L-lysine, 0.09 part of DL-methionine, 0.09 part of L-tryptophan, 0.28 part of L-threonine, 0.41 part of glucose, 0.45 part of fermentation broth preparation, 0.04 part of phytase, 0.04 part of complex enzyme preparation, 0.04 part of lactic acid extract, 0.40 part of plant oligopeptide, 0.45 part of plant-oriented peptide, 0.45 part of shell-producing polypeptide, 0.55 part of evodia, 0.02 part of Chinese medicinal herb, 0.55 part of Chinese wampee preparation, 0.45 part of Chinese wampe, 0.25 part of spina date seed, 0.25 part of paniculate swallowwort root, 0.25 part of three-paired burls, 0.25 part of phyllanthus urinaria and 0.12 part of honey-fried licorice root.
The fermentation bacteria preparation is a mixture prepared from lactobacillus plantarum, bacillus, saccharomyces cerevisiae, trichoderma viride, bifidobacterium bifidum and candida utilis, the number of live bacteria of each bacteria is not specifically required, and the total number of the live bacteria reaches 125 hundred million/g.
The weight percentage of crude protein of the expanded cyperus esculentus bean pulp is 15.41 percent, the weight percentage of crude protein of steam-exploded peanut vine powder is 7.81 percent, the weight percentage of crude protein of steam-exploded bean curd residue is 22.53 percent, the weight percentage of crude protein of expanded leather powder is 74.54 percent, the weight percentage of crude protein of expanded brown rice powder is 8.86 percent, the weight percentage of crude protein of expanded peanut kernel pulp is 47.53 percent, the weight percentage of crude protein of beer yeast is 74.56 percent, the weight percentage of crude protein of fish meal is 60.25 percent, the weight percentage of crude protein of expanded corn is 8.74 percent, the weight percentage of crude protein of expanded full-fat soybean is 35.53 percent, the weight percentage of crude protein of whey powder is 22.52 percent, the total weight percentage of eicosapentaenoic acid and docosahexaenoic acid of fish oil is 20.05 percent, the weight percentage of calcium carbonate is 38.08 percent, the weight percentage of calcium phosphate is 18.06 percent, the weight percentage of L-lysine is 70.0 percent, the DL-methionine-serine, the weight percentage of chitosan-tryptophan-serine-lyase extract is 0.460 percent, the weight percentage of mixed enzyme activity of L-xylanase, the enzyme preparation is prepared by weight percentage of xylanase, the enzyme preparation with the weight percentage of xylanase, the enzyme activity of xylanase, the enzyme preparation with the enzyme activity of xylanase, the weight percentage of xylanase, the enzyme preparation with the weight percentage of xylanase, the enzyme preparation with the content of xylanase, the weight percentage of xylanase, the enzyme preparation with the enzyme preparation.
The preparation method of the high-efficiency grain-saving immune fermented compound feed for the weaned piglets comprises the following specific steps:
(1) weighing specified parts by weight of puffed cyperus esculentus seed meal, steam-exploded peanut vine powder, steam-exploded bean curd residue, puffed leather powder, puffed brown rice powder, beer yeast, fish meal, puffed corn, puffed full-fat soybean, peony, fructus evodiae-processed coptis chinensis, cinnamon, spina date seed, paniculate swallowwort root, three-paired knots, phyllanthus urinaria and honey-fried licorice root, and primarily mixing; then, crushing for the first time by a crusher and sieving by a 12-mesh sieve, crushing for the second time by an ultrafine crusher and sieving by a 80-mesh sieve to prepare an ultrafine crushing mixture;
(2) weighing whey powder, calcium carbonate, calcium hydrophosphate, salt, sodium bicarbonate, a microelement compound premix, a vitamin compound premix, L-lysine, DL-methionine, L-tryptophan, L-threonine, phytase, a compound enzyme preparation, a plant extract, an antibacterial peptide preparation and oligochitosan in specified weight parts, stirring and mixing uniformly to prepare a small material premix;
(3) weighing water, glucose, lactic acid, a zymocyte preparation and crushed 60-mesh puffed peanut kernel meal in specified weight parts, pouring the mixture into a clean container, uniformly stirring, carrying out constant-temperature shaking culture at 37 ℃ for 3.5 hours, and activating the zymocyte preparation to prepare a strain activation solution;
(4) weighing fish oil in a specified weight part, and uniformly stirring and mixing the fish oil, the superfine grinding mixture, the small material premix and the strain activation liquid to prepare a fermented compound feed initial substance;
(5) putting the fermented compound feed initial material into a closed container or a fermentation bag, performing anaerobic fermentation (sealing), and standing at 30 deg.C for 4.5 days to obtain fermented compound feed.
Example 2
An efficient grain-saving immune fermented compound feed for weaned piglets is prepared by solid-state fermentation of the following raw materials in parts by weight: 25.75 parts of water, 8.29 parts of expanded cyperus bean pulp, 2.56 parts of steam-exploded peanut vine meal, 1.64 parts of steam-exploded bean curd residue, 2.75 parts of expanded leather powder, 8.01 parts of expanded brown rice powder, 4.25 parts of expanded peanut kernel meal, 3.25 parts of beer yeast, 2.75 parts of fish meal, 48.28 parts of expanded corn, 2.01 parts of expanded full-fat soybean, 3.50 parts of whey powder, 5.25 parts of fish oil, 0.32 part of calcium carbonate, 0.67 part of calcium hydrophosphate, 0.30 part of salt, 0.32 part of sodium bicarbonate, 0.15 part of microelement compound premix, 0.15 part of vitamin compound premix, 0.84 part of L-lysine, 0.10 part of DL-methionine, 0.08 part of L-tryptophan, 0.29 part of L-threonine, 0.42 part of glucose, 0.45 part of fermentation starter preparation, 0.03 part of phytase, 0.02 part of complex enzyme preparation, 0.10 part of lactic acid extract, 0.35 part of plant oligopeptide, 0.03 part of plant-oriented peptide, 0.53 part of shell-producing polypeptide, 0.44.53 parts of evodia, 0.44 parts of Chinese medicinal preparation, 0.44 parts of Chinese goldthread, 0.7 parts of Chinese wa, 0.24 part of spina date seed, 0.24 part of paniculate swallowwort root, 0.24 part of three-paired burls, 0.24 part of phyllanthus urinaria and 0.11 part of honey-fried licorice root.
The fermentation bacteria preparation is a mixture prepared from lactobacillus plantarum, bacillus, saccharomyces cerevisiae, trichoderma viride, bifidobacterium bifidum and enterococcus faecalis, the number of live bacteria of each bacteria is not specifically required, and the total number of live bacteria reaches 125 hundred million/g.
The weight percentage of crude protein of the expanded cyperus esculentus bean pulp is 15.43%, the weight percentage of crude protein of steam-exploded peanut vine meal is 7.84%, the weight percentage of crude protein of steam-exploded bean curd residue is 22.55%, the weight percentage of crude protein of expanded leather powder is 74.56%, the weight percentage of crude protein of expanded brown rice powder is 8.83%, the weight percentage of crude protein of expanded peanut kernel meal is 47.56%, the weight percentage of crude protein of beer yeast is 74.57%, the weight percentage of fish meal is 60.23%, the weight percentage of expanded corn is 8.72%, the weight percentage of crude protein of expanded full-fat soybean is 35.58%, the weight percentage of crude protein of whey powder is 22.55%, the total weight percentage of eicosapentaenoic acid and docosahexaenoic acid of fish oil is 20.08%, the weight percentage of calcium carbonate is 38.05%, the weight percentage of calcium phosphate is 18.06%, the weight percentage of L-lysine content is 70.0%, the weight percentage of DL-methionine, the weight percentage of chitosan-methionine, the weight percentage of L-tryptophan, the L-serine, the L-xylanase, the serine, the xylanase, the L-xylanase, the xylanase.
The preparation method of the high-efficiency grain-saving immune fermented compound feed for the weaned piglets comprises the following specific steps:
(1) weighing expanded cyperus esculentus meal, steam-exploded peanut vine powder, steam-exploded bean curd residue, expanded leather powder, expanded brown rice powder, beer yeast, fish meal, expanded corn, expanded whole-fat soybean, Chinese herbaceous peony, evodia fruit-processed coptis chinensis, cinnamon, spina date seed, paniculate swallowwort root, three-joint, phyllanthus urinaria and honey-fried licorice root in specified weight, and primarily mixing; then crushing and sieving for the first time by a crusher with 12 meshes, crushing and sieving for the second time by an ultrafine crusher with 80 meshes to prepare an ultrafine crushing mixture;
(2) weighing whey powder, calcium carbonate, calcium hydrophosphate, salt, sodium bicarbonate, a microelement compound premix, a vitamin compound premix, L-lysine, DL-methionine, L-tryptophan, L-threonine, phytase, a compound enzyme preparation, a plant extract, an antibacterial peptide preparation and oligomeric chitosan according to specified weight, stirring and mixing uniformly to prepare a small material premix;
(3) weighing water, glucose, lactic acid, a zymocyte preparation and crushed 60-mesh puffed peanut kernel meal, pouring the mixture into a clean container, uniformly stirring, carrying out constant-temperature shaking culture at 37 ℃ for 3.5 hours, and activating the zymocyte preparation to prepare a strain activation solution;
(4) weighing fish oil with specified weight, and uniformly stirring and mixing the fish oil with the superfine grinding mixture, the small material premix and the strain activation liquid to prepare a fermented compound feed initial substance;
(5) putting the fermented compound feed initial material into a closed container or a fermentation bag, performing anaerobic fermentation (sealing), and standing at 33 deg.C for 4.0 days to obtain fermented compound feed.
Example 3
An efficient grain-saving immune fermented compound feed for weaned piglets is prepared by solid-state fermentation of the following raw materials in parts by weight: 25.50 parts of water, 8.64 parts of expanded cyperus bean pulp, 3.18 parts of steam-exploded peanut vine meal, 1.99 parts of steam-exploded bean curd residue, 2.50 parts of expanded leather powder, 8.77 parts of expanded brown rice powder, 4.50 parts of expanded peanut kernel meal, 3.00 parts of beer yeast, 2.50 parts of fish meal, 46.32 parts of expanded corn, 2.80 parts of expanded full-fat soybean, 3.00 parts of whey powder, 5.42 parts of fish oil, 0.31 part of calcium carbonate, 0.70 part of calcium hydrophosphate, 0.30 part of salt, 0.30 part of sodium bicarbonate, 0.15 part of microelement composite premix, 0.15 part of vitamin composite premix, 0.83 part of L-lysine, 0.11 part of DL-methionine, 0.08 part of L-tryptophan, 0.29 part of L-threonine, 0.43 part of glucose, 0.45 part of zymocyte preparation, 0.03 part of phytase, 0.03 part of complex enzyme preparation, 0.03 part of lactic acid extract, 0.10 part of plant oligopeptide, 0.43 part of plant peptide, 0.40 part of shell-producing polypeptide, 0.40 parts of evodia rutaecarpa preparation, 0.40 parts of Chinese goldthread root, 0.15 parts of fructus evodiae, 0.15 parts of, 0.23 part of spina date seed, 0.23 part of paniculate swallowwort root, 0.23 part of three-paired burls, 0.23 part of phyllanthus urinaria and 0.10 part of honey-fried licorice root.
The fermentation bacteria preparation is a mixture prepared from lactobacillus plantarum, bacillus, saccharomyces cerevisiae, trichoderma viride, bifidobacterium bifidum and candida albicans, the number of live bacteria of each bacterium is not specifically required, and the total number of the live bacteria reaches 130 hundred million/g.
15.42 percent of crude protein of expanded cyperus esculentus bean pulp, 7.81 percent of crude protein of steam exploded peanut vine powder, 22.52 percent of crude protein of steam exploded bean curd residue, 74.54 percent of expanded leather powder, 8.85 percent of expanded brown rice powder, 47.53 percent of expanded peanut kernel meal, 74.58 percent of brewer's yeast, 60.29 percent of fish meal, 8.75 percent of expanded corn, 35.54 percent of expanded full-fat soybean, 22.57 percent of whey powder, 20.09 percent of fish oil eicosapentaenoic acid and docosahexaenoic acid, 38.04 percent of calcium carbonate, 18.02 percent of calcium hydrophosphate, 70.0 percent of L-lysine, 99.0 percent of DL-methionine, 99.0-99 percent of L-tryptophan, 0-5 percent of mixed enzyme with serine, 250 percent of cellulase, 250 percent of phytase, 250.00 percent of chitosan, 250.00 percent of cellulase, 250.00 percent of chitosan-serine, 250 percent of cellulase, 250 percent of chitosan-10 percent of cellulase, 250-70 percent of cellulase, 250-10 percent of phytase, 250 percent of cellulase, 250 percent of phytase, 250 percent of cellulase, 250-10 percent of xylanase, 250-10 percent of cellulase, 4 percent of xylanase, and 10 percent of cellulase.
The preparation method of the high-efficiency grain-saving immune fermented compound feed for the weaned piglets comprises the following specific steps:
(1) weighing expanded cyperus esculentus meal, steam-exploded peanut vine powder, steam-exploded bean curd residue, expanded leather powder, expanded brown rice powder, beer yeast, fish meal, expanded corn, expanded whole-fat soybean, Chinese herbaceous peony, evodia fruit-processed coptis chinensis, cinnamon, spina date seed, paniculate swallowwort root, three-joint, phyllanthus urinaria and honey-fried licorice root in specified weight, and primarily mixing; then crushing and sieving for the first time by a crusher with 12 meshes, crushing and sieving for the second time by an ultrafine crusher with 80 meshes to prepare an ultrafine crushing mixture;
(2) weighing whey powder, calcium carbonate, calcium hydrophosphate, salt, sodium bicarbonate, a microelement compound premix, a vitamin compound premix, L-lysine, DL-methionine, L-tryptophan, L-threonine, phytase, a compound enzyme preparation, a plant extract, an antibacterial peptide preparation and oligomeric chitosan according to specified weight, stirring and mixing uniformly to prepare a small material premix;
(3) weighing water, glucose, lactic acid, a zymocyte preparation and crushed 60-mesh puffed peanut kernel meal, pouring the mixture into a clean container, uniformly stirring, carrying out constant-temperature shaking culture at 37 ℃ for 3.5 hours, and activating the zymocyte preparation to prepare a strain activation solution;
(4) weighing fish oil with specified weight, and uniformly stirring and mixing the fish oil with the superfine grinding mixture, the small material premix and the strain activation liquid to prepare a fermented compound feed initial substance;
(5) putting the fermented compound feed initial material into a closed container or a fermentation bag, performing anaerobic fermentation (sealing), and standing at 36 deg.C for 4.0 days to obtain fermented compound feed.
Example 4
An efficient grain-saving immune fermented compound feed for weaned piglets is prepared by solid-state fermentation of the following raw materials in parts by weight: 25.75 parts of water, 8.98 parts of expanded cyperus bean pulp, 3.80 parts of steam-exploded peanut vine meal, 2.33 parts of steam-exploded bean curd residue, 2.25 parts of expanded leather powder, 9.56 parts of expanded brown rice powder, 4.75 parts of expanded peanut kernel meal, 2.75 parts of beer yeast, 2.25 parts of fish meal, 44.35 parts of expanded corn, 3.60 parts of expanded full-fat soybean, 2.50 parts of whey powder, 5.58 parts of fish oil, 0.30 part of calcium carbonate, 0.73 part of calcium hydrophosphate, 0.30 part of salt, 0.28 part of sodium bicarbonate, 0.15 part of microelement composite premix, 0.15 part of vitamin composite premix, 0.82 part of L-lysine, 0.11 part of DL-methionine, 0.07 part of L-tryptophan, 0.30 part of L-threonine, 0.44 part of glucose, 0.45 part of fermentation broth preparation, 0.02 part of phytase, 0.03 part of complex enzyme preparation, 0.10 part of lactic acid extract, 0.25 part of plant oligopeptide, 0.45 part of plant-oriented peptide, 0.41 part of shell-producing polypeptide, 0.41 part of evodia, 0.41 part of Chinese medicinal herb, 0.41 parts of Chinese goldthread, 0.21 part of spina date seed, 0.21 part of paniculate swallowwort root, 0.21 part of three-paired burls, 0.21 part of phyllanthus urinaria and 0.09 part of honey-fried licorice root.
The fermentation bacteria preparation is a mixture prepared from lactobacillus plantarum, bacillus, saccharomyces cerevisiae, trichoderma viride, candida utilis and enterococcus faecalis, the number of live bacteria of each bacteria is not specifically required, and the total number of the live bacteria reaches 135 hundred million/g.
The weight percentage of crude protein of the expanded cyperus esculentus bean pulp is 15.45%, the weight percentage of crude protein of steam-exploded peanut vine meal is 7.82%, the weight percentage of crude protein of steam-exploded bean curd residue is 22.54%, the weight percentage of crude protein of expanded leather powder is 74.52%, the weight percentage of crude protein of expanded brown rice powder is 8.89%, the weight percentage of crude protein of expanded peanut kernel meal is 47.54%, the weight percentage of crude protein of beer yeast is 74.53%, the weight percentage of crude protein of fish meal is 60.26%, the weight percentage of expanded corn is 8.78%, the weight percentage of crude protein of expanded full-fat soybean is 35.55%, the weight percentage of crude protein of whey powder is 22.52%, the total weight percentage of eicosapentaenoic acid and docosahexaenoic acid of fish oil is 20.08%, the weight percentage of calcium carbonate is 38.02%, the weight percentage of calcium phosphate is 18.06%, the weight percentage of L-lysine content is 70.0%, the weight percentage of DL-methionine, the weight percentage of threonine content is 99.02%, the weight percentage of calcium carbonate content of calcium phosphate is 18.06%, the weight percentage of the L-lysine content of L-lysine is 70.0%, the L-lysine content of chitosan, the L-lysine content of chitosan-lysine is 70%, the L-lysine content of chitosan-lysine, the L-lysine-serine, the L-serine-chitosanase of the L-serine-xylanase, the L-xylanase extract is 0.00%, the L-20%, the L-serine enzyme of the xylanase, the xylanase of the xylanase, the xylanase of.
The preparation method of the high-efficiency grain-saving immune fermented compound feed for the weaned piglets comprises the following specific steps:
(1) weighing expanded cyperus esculentus meal, steam-exploded peanut vine powder, steam-exploded bean curd residue, expanded leather powder, expanded brown rice powder, beer yeast, fish meal, expanded corn, expanded whole-fat soybean, Chinese herbaceous peony, evodia fruit-processed coptis chinensis, cinnamon, spina date seed, paniculate swallowwort root, three-joint, phyllanthus urinaria and honey-fried licorice root in specified weight, and primarily mixing; then crushing and sieving for the first time by a crusher with 12 meshes, crushing and sieving for the second time by an ultrafine crusher with 80 meshes to prepare an ultrafine crushing mixture;
(2) weighing whey powder, calcium carbonate, calcium hydrophosphate, salt, sodium bicarbonate, a microelement compound premix, a vitamin compound premix, L-lysine, DL-methionine, L-tryptophan, L-threonine, phytase, a compound enzyme preparation, a plant extract, an antibacterial peptide preparation and oligomeric chitosan according to specified weight, stirring and mixing uniformly to prepare a small material premix;
(3) weighing water, glucose, lactic acid, a zymocyte preparation and crushed 60-mesh puffed peanut kernel meal, pouring the mixture into a clean container, uniformly stirring, carrying out constant-temperature shaking culture at 37 ℃ for 3.5 hours, and activating the zymocyte preparation to prepare a strain activation solution;
(4) weighing fish oil with specified weight, and uniformly stirring and mixing the fish oil with the superfine grinding mixture, the small material premix and the strain activation liquid to prepare a fermented compound feed initial substance;
(5) putting the fermented compound feed initial material into a closed container or a fermentation bag, performing anaerobic fermentation (sealing), and standing at 39 deg.C for 3.5 days to obtain fermented compound feed.
Example 5
An efficient grain-saving immune fermented compound feed for weaned piglets is prepared by solid-state fermentation of the following raw materials in parts by weight: 26.00 parts of water, 9.33 parts of expanded cyperus bean pulp, 4.42 parts of steam-exploded peanut vine meal, 2.68 parts of steam-exploded bean curd residue, 2.00 parts of expanded leather powder, 10.41 parts of expanded brown rice powder, 5.00 parts of expanded peanut kernel meal, 2.50 parts of beer yeast, 2.00 parts of fish meal, 42.38 parts of expanded corn, 4.39 parts of expanded full-fat soybean, 2.00 parts of whey powder, 5.74 parts of fish oil, 0.29 part of calcium carbonate, 0.76 part of calcium hydrophosphate, 0.30 part of salt, 0.25 part of sodium bicarbonate, 0.15 part of microelement compound premix, 0.15 part of vitamin compound premix, 0.81 part of L-lysine, 0.12 part of DL-methionine, 0.06 part of L-tryptophan, 0.30 part of L-threonine, 0.45 part of glucose, 0.45 part of fermentation starter preparation, 0.02 part of phytase, 0.04 part of compound enzyme preparation, 0.10 part of lactic acid extract, 0.20 part of plant oligopeptide, 0.04 part of chitosan preparation, 0.40 part of shell-producing polypeptide, 0.45 part of evodia, 0.40 parts of Chinese medicinal herb, 0.45 part of Chinese wampee, 0.20 part of spina date seed, 0.20 part of paniculate swallowwort root, 0.20 part of three-paired burls, 0.20 part of phyllanthus urinaria and 0.08 part of honey-fried licorice root.
The fermentation bacteria preparation is a mixture prepared from lactobacillus plantarum, bacillus, saccharomyces cerevisiae, trichoderma viride, candida utilis and candida albicans, the number of live bacteria of each bacteria is not specifically required, and the total number of the live bacteria reaches 140 hundred million/g.
The weight percentage of crude protein of the expanded cyperus esculentus bean pulp is 15.48%, the weight percentage of crude protein of steam exploded peanut vine meal is 7.85%, the weight percentage of crude protein of steam exploded bean curd residue is 22.57%, the weight percentage of crude protein of expanded leather powder is 74.52%, the weight percentage of crude protein of expanded brown rice powder is 8.85%, the weight percentage of crude protein of expanded peanut kernel meal is 47.52%, the weight percentage of crude protein of beer yeast is 74.59%, the weight percentage of crude protein of fish meal is 60.22%, the weight percentage of crude protein of expanded corn is 8.75%, the weight percentage of crude protein of expanded full-fat soybean is 35.51%, the weight percentage of crude protein of whey powder is 22.57%, the total weight percentage of eicosapentaenoic acid and docosahexaenoic acid of fish oil is 20.09%, the weight percentage of calcium carbonate is 38.08%, the weight percentage of calcium phosphate is 18.03%, the weight percentage of L-lysine content is 70.0%, the weight percentage of DL-methionine-serine, the weight percentage of L-tryptophan-serine, the weight percentage of chitosan-serine-lyase-cellulase preparation is 90%, the weight percentage of mixed enzyme preparation is composed of xylanase, the enzyme preparation with the weight percentage of xylanase, the enzyme preparation is composed of xylanase, the enzyme preparation with the weight percentage of xylanase, the enzyme activity of xylanase, the enzyme preparation is 90.10%, the enzyme preparation is composed of xylanase, the enzyme preparation with the weight percentage of xylanase, the enzyme preparation is 90.10.10%, the enzyme preparation is composed of xylanase, the enzyme preparation with the weight percentage of xylanase, the enzyme preparation is 90.10%, the weight percentage of the enzyme preparation, the weight percentage.
The preparation method of the high-efficiency grain-saving immune fermented compound feed for the weaned piglets comprises the following specific steps:
(1) weighing expanded cyperus esculentus meal, steam-exploded peanut vine powder, steam-exploded bean curd residue, expanded leather powder, expanded brown rice powder, beer yeast, fish meal, expanded corn, expanded whole-fat soybean, Chinese herbaceous peony, evodia fruit-processed coptis chinensis, cinnamon, spina date seed, paniculate swallowwort root, three-joint, phyllanthus urinaria and honey-fried licorice root in specified weight, and primarily mixing; then crushing and sieving for the first time by a crusher with 12 meshes, crushing and sieving for the second time by an ultrafine crusher with 80 meshes to prepare an ultrafine crushing mixture;
(2) weighing whey powder, calcium carbonate, calcium hydrophosphate, salt, sodium bicarbonate, a microelement compound premix, a vitamin compound premix, L-lysine, DL-methionine, L-tryptophan, L-threonine, phytase, a compound enzyme preparation, a plant extract, an antibacterial peptide preparation and oligomeric chitosan according to specified weight, stirring and mixing uniformly to prepare a small material premix;
(3) weighing water, glucose, lactic acid, a zymocyte preparation and crushed 60-mesh puffed peanut kernel meal, pouring the mixture into a clean container, uniformly stirring, carrying out constant-temperature shaking culture at 37 ℃ for 3.5 hours, and activating the zymocyte preparation to prepare a strain activation solution;
(4) weighing fish oil with specified weight, and uniformly stirring and mixing the fish oil with the superfine grinding mixture, the small material premix and the strain activation liquid to prepare a fermented compound feed initial substance;
(5) putting the fermented compound feed initial material into a sealed container or a fermentation bag, performing anaerobic fermentation (sealing), and standing at 42 deg.C for 3.0 days to obtain fermented compound feed.
Test examples
According to the principle that sex, times of birth, weight and blood relationship are similar, 180 21-day old Du-Dai three-way hybrid weaned piglets are selected and randomly divided into 6 groups, each group is provided with 30 pigs, each group is provided with 5 times of repetition, 6 pigs are repeated each time, the test diets of a control group and a test group (examples 1-5) are randomly fed, and feeding tests are carried out for 42 days. The pigs are fed 4 times a day, are free to drink water and feed, and are immunized, disinfected, cleaned and sanitary and have the function of expelling parasites according to the normal method of a pig farm.
The control group was fed with commercial weaned piglet compound feed (corn-soybean meal-fish meal type, high in market occupation rate, containing oxytetracycline and flavomycin), the feed formula of which is shown in table 1; the test components are respectively fed to the weaned pig high-efficiency grain-saving immune type fermented compound feed prepared in the embodiments 1-5 of the invention.
TABLE 1 commercial weaned piglet Compound feed formula
Figure BDA0002433469910000151
Figure BDA0002433469910000161
Note: the compound premix is a special compound premix for weaned piglets produced by Zhan shoukang feed Limited company in Xinxiang city, and contains oxytetracycline and flavomycin.
After the feeding test is finished, the following indexes are measured, the test process of SAS statistical software is used for carrying out T test by taking test treatment as a unit, and the significance of the data difference between the test group and the control group is judged. Statistical results are expressed as mean ± standard deviation.
(1) Index of production Performance
Average daily gain: the average daily gain of each treatment group was counted by weighing the animals on empty stomach column by column on the day of the start of the test and in the early morning of 42 days, respectively.
Average daily food intake: the feed consumption of each replicate group was accurately recorded during the trial period, the total and average daily feed intake (on a 87.5% dry matter basis, the same applies hereinafter) was calculated for each replicate group piglet, and the average daily feed intake was calculated for each trial group piglet.
The material weight ratio is as follows: and calculating the feed-weight ratio according to the feed intake and the weight gain of the piglets of each treatment group.
The results of the weaned piglet productivity test are shown in table 2.
TABLE 2 weaned piglet Productivity test results
Figure BDA0002433469910000162
Note: the data in the same row of the table are marked with significant differences (P <0.05), as follows.
The results in table 2 show that the average daily gain of examples 1 to 5 is significantly increased compared to the control group, and the increase ranges are 20.12%, 19.09%, 20.54%, 19.50% and 19.92%, respectively (P < 0.05); the average daily food intake is obviously increased by 11.63%, 12.08%, 12.37%, 11.34% and 12.22% (P < 0.05); the material-weight ratio is obviously improved, and the improvement ranges are respectively 7.56%, 6.43%, 7.20%, 7.27% and 6.92% (P is less than 0.05). The result shows that the high-efficiency grain-saving immune fermented compound feed for the weaned pigs replaces commercial compound feed for the weaned pigs, and can obviously improve the average daily gain, the average daily feed intake and the feed-weight ratio of the weaned pigs.
(2) Indexes of diarrhea rate, morbidity and survival rate
During the test period, the health condition of the pigs is observed and recorded every day, diarrhea individuals and diseased pig individuals describing the pigs and the duration time of the diarrhea individuals and the diseased pig individuals are recorded, and the diarrhea rate and the disease rate of piglets in the test period are counted by taking repetition as a unit.
The diarrhea rate is (total diarrhea piglet head number × piglet diarrhea days)/(experimental piglet head number × experimental days) × 100%
The incidence rate is (sum of number of affected piglets/total number of piglets x number of test days) x 100%.
The diarrhea rate and incidence test results of weaned piglets are shown in table 3.
TABLE 3 incidence, diarrhea and survival rate of weaned piglets
Figure BDA0002433469910000171
The results in table 3 show that the diarrhea rate of examples 1-5 is significantly reduced compared with the control group, and the reduction range is 64.60%, 54.77%, 48.00%, 44.72% and 58.41% (P < 0.05); the morbidity is obviously reduced, and the reduction range respectively reaches 58.67%, 50.85%, 49.25%, 54.39% and 53.36% (P is less than 0.05); the survival rate is 100 percent. The result shows that the high-efficiency grain-saving immune fermented compound feed for the weaned pigs replaces commercial compound feed for the weaned pigs, the survival rate of the weaned pigs reaches 100 percent, and the morbidity and diarrhea rate of the weaned pigs can be obviously reduced.
(3) Hog cholera antibody index in serum
The indexes of the hog cholera antibody in serum are determined by using a detection kit (purchased from Shanghai Zhen Ke Biotech Co., Ltd.) for the hog cholera virus (CSFV) antibody. The results of the swine fever antibody level test in serum are shown in Table 4.
TABLE 4 serum hog cholera antibody level test results
Figure BDA0002433469910000181
The results in table 4 show that the level of swine fever antibodies in the serum of examples 1-5 is significantly increased by 74.11%, 85.71%, 88.39%, 102.68%, and 90.18% (P <0.05) compared with the control group at days 1-14; the swine fever antibody level in the serum of 15-28 days is obviously improved, and the improvement range is 79.70%, 73.68%, 82.71%, 70.68% and 84.96% (P <0.05) respectively. The result shows that the high-efficiency grain-saving immune fermented compound feed for the weaned pigs replaces commercial weaned pig compound feed, and can obviously improve the level of the swine fever antibodies in serum.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims (6)

1. An efficient grain-saving immune fermented compound feed for weaned piglets is characterized by being prepared by solid-state fermentation of the following raw materials in parts by weight: 25.00 to 26.00 parts of water, 7.94 to 9.33 parts of expanded cyperus bean pulp, 1.94 to 4.42 parts of steam-exploded peanut meal, 1.29 to 2.68 parts of steam-exploded bean curd residue, 2.00 to 3.00 parts of expanded leather powder, 7.11 to 10.41 parts of expanded brown rice powder, 4.00 to 5.00 parts of expanded peanut kernel meal, 2.50 to 3.50 parts of beer yeast, 2.00 to 3.00 parts of fish meal, 0.64 to 0.25 part of expanded corn 42.38, 1.21 to 4.39 parts of expanded full-fat soybean, 2.00 to 4.00 parts of whey powder, 5.09 to 5.74 parts of fish oil, 0.29 to 0.33 part of calcium carbonate, 0.64 to 0.76 part of calcium hydrophosphate, 0.30 part of salt, 0.25 to 0.35 part of sodium bicarbonate, 0.15 part of microelement compound premix, 0.15 part of vitamin compound premix, 0.81 to 0.81 part of L-0.85 part of L-0.09 to 0.12.12 parts of L-L tryptophan, 0.06 part of L-0.09 to 0.35 part of L-0.06 part of L-L threonine, 0.41-0.45 part of glucose, 0.45 part of zymocyte preparation, 0.02-0.04 part of phytase, 0.02-0.04 part of complex enzyme preparation, 0.10 part of lactic acid, 0.20-0.40 part of plant extract, 0.02-0.04 part of antibacterial peptide preparation, 0.30-0.50 part of oligomeric chitosan, 0.45-0.55 part of peony, 0.40-0.45 part of fructus evodiae rhizoma coptidis, 0.40-0.45 part of cinnamon, 0.20-0.25 part of spina date seed, 0.20-0.25 part of paniculate swallowwort root, 0.20-0.25 part of three-paired nodes, 0.20-0.25 part of phyllanthus urinaria and 0.08-0.12 part of honey-fried licorice root.
2. The efficient grain-saving immune fermented compound feed for weaned pigs according to claim 1, wherein the fermentation bacteria preparation is prepared by adding any two of bifidobacterium bifidum, candida utilis, enterococcus faecalis and candida albicans on the basis of lactobacillus plantarum, bacillus, saccharomyces cerevisiae and trichoderma viride, and the total number of viable bacteria is more than 120 hundred million/g.
3. The efficient grain-saving immune type fermented compound feed for weaned pigs according to claim 2, characterized in that the mass content of crude protein of the puffed cyperus esculentus meal is more than 15.40%; the mass content of crude protein of the steam-exploded peanut vine powder is more than 7.80 percent; the mass content of the crude protein of the steam-exploded bean curd residue is more than 22.50 percent; the mass content of the crude protein of the expanded leather powder is above 74.50%; the mass content of crude protein of the puffed brown rice powder is more than 8.80 percent; the mass content of the crude protein of the puffed peanut kernel meal is above 47.50%; the mass content of the crude protein of the beer yeast is above 74.50%; the mass content of the crude protein of the fish meal is more than 60.20 percent; the mass content of the crude protein of the puffed corn is more than 8.70 percent; the mass content of the crude protein of the puffed full-fat soybean is more than 35.50 percent; the crude protein mass content of the whey powder is more than 22.50 percent; the total mass content of eicosapentaenoic acid and docosahexaenoic acid of the fish oil is more than 20.00 percent; the calcium content of the calcium carbonate is more than 38.00 percent; the phosphorus content of the calcium hydrophosphate is more than 18.00 percent by mass; the mass content of L-lysine in the L-lysine is 70.0 percent; the DL-methionine content of DL-methionine is 99.0%; the mass content of L-tryptophan in the L-tryptophan is 99.0 percent; the L-threonine mass content of the L-threonine is 99.0%; the glucose mass content of the glucose is more than 98.0 percent; the phytase activity of the phytase is more than 2000U/g; the lactic acid mass content of the lactic acid is more than 98.0 percent; the oligochitosan of the oligochitosan has the mass content of more than 99.0 percent.
4. The efficient grain-saving immune type fermented compound feed for the weaned pigs according to claim 3, wherein the compound enzyme preparation is prepared by mixing xylanase, protease, amylase, β -mannase, cellulase and β -glucanase, the xylanase activity in the compound enzyme preparation is over 12000U/g, the protease activity is over 5200U/g, the amylase activity is over 180000U/g, the β -mannase activity is over 450U/g, the cellulase activity is over 9000U/g and the β -glucanase activity is over 22000U/g.
5. The efficient grain-saving immune type fermented compound feed for weaned pigs according to claim 4, wherein the plant extract is a uniform mixture consisting of 50% of honeysuckle branch and leaf extract, 20% of gynostemma pentaphylla extract, 20% of momordica grosvenori extract and 10% of rabdosia rubescens extract.
6. The preparation method of the high-efficiency grain-saving immune type fermented compound feed for weaned pigs according to any one of claims 1-5, which is characterized by comprising the following specific steps:
(1) weighing specified parts by weight of puffed cyperus esculentus seed meal, steam-exploded peanut vine powder, steam-exploded bean curd residue, puffed leather powder, puffed brown rice powder, beer yeast, fish meal, puffed corn, puffed full-fat soybean, peony, fructus evodiae-processed coptis chinensis, cinnamon, spina date seed, paniculate swallowwort root, three-paired knots, phyllanthus urinaria and honey-fried licorice root, and primarily mixing; then, crushing for the first time by a crusher and sieving by a 12-mesh sieve, crushing for the second time by an ultrafine crusher and sieving by a 80-mesh sieve to prepare an ultrafine crushing mixture;
(2) weighing whey powder, calcium carbonate, calcium hydrophosphate, salt, sodium bicarbonate, a microelement compound premix, a vitamin compound premix, L-lysine, DL-methionine, L-tryptophan, L-threonine, phytase, a compound enzyme preparation, a plant extract, an antibacterial peptide preparation and oligochitosan in specified weight parts, stirring and mixing uniformly to prepare a small material premix;
(3) weighing water, glucose, lactic acid, a zymocyte preparation and crushed 60-mesh puffed peanut kernel meal in specified weight parts, pouring the mixture into a clean container, uniformly stirring, carrying out constant-temperature shaking culture at 37 ℃ for 3.5 hours, and activating the zymocyte preparation to prepare a strain activation solution;
(4) weighing fish oil in a specified weight part, and uniformly stirring and mixing the fish oil with the superfine grinding mixture prepared in the step (1), the small material premix prepared in the step (2) and the strain activation liquid prepared in the step (3) to prepare a fermented compound feed initial substance;
(5) and (3) putting the initial material of the fermented compound feed prepared in the step (4) into a closed container or a fermentation bag, performing anaerobic fermentation, and standing for 3.0-4.5 days at the temperature of 30-42 ℃ to prepare a finished product of the fermented compound feed.
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3928633A1 (en) * 2020-06-24 2021-12-29 Tangrenshen Group Co., Ltd. Preparation method of bio-fermented liquid feed, bio-fermented liquid feed and bio-fermented emulsion liquid feed
CN114391603A (en) * 2022-01-28 2022-04-26 河南省科学院生物研究所有限责任公司 Feeding traditional Chinese medicine microecological preparation and preparation method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102805204A (en) * 2012-07-26 2012-12-05 周建设 Biological fermentation forage grass containing Chinese herbal medicines and used for raising pigs
CN103141710A (en) * 2013-04-02 2013-06-12 山东新希望六和集团有限公司 Compound feed for weaned pigs and preparation method of feed
CN105614115A (en) * 2016-03-14 2016-06-01 河南科技学院 Weaned piglet healthy and environment-friendly fermented mixed feed

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102805204A (en) * 2012-07-26 2012-12-05 周建设 Biological fermentation forage grass containing Chinese herbal medicines and used for raising pigs
CN103141710A (en) * 2013-04-02 2013-06-12 山东新希望六和集团有限公司 Compound feed for weaned pigs and preparation method of feed
CN105614115A (en) * 2016-03-14 2016-06-01 河南科技学院 Weaned piglet healthy and environment-friendly fermented mixed feed

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3928633A1 (en) * 2020-06-24 2021-12-29 Tangrenshen Group Co., Ltd. Preparation method of bio-fermented liquid feed, bio-fermented liquid feed and bio-fermented emulsion liquid feed
CN114391603A (en) * 2022-01-28 2022-04-26 河南省科学院生物研究所有限责任公司 Feeding traditional Chinese medicine microecological preparation and preparation method thereof

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