CN105995249A

CN105995249A - Banana prawn feed additive and preparation method thereof

Info

Publication number: CN105995249A
Application number: CN201610626332.XA
Authority: CN
Inventors: 燕磊; 吕明斌; 黄河
Original assignee: Shandong New Hope Liuhe Group Co Ltd
Current assignee: Shandong New Hope Liuhe Group Co Ltd
Priority date: 2016-08-02
Filing date: 2016-08-02
Publication date: 2016-10-12

Abstract

The invention provides a banana prawn feed additive and a preparation method thereof. The additive comprises the following components: Andrias davidianus oil, purslane, papaya, dandelion, loofah, dried tangerine peel, lily, lentinus edodes, mint, mulberry leaf, kiwi fruit, Epilobium pyrricholophum herb, Alysicarpus vaginalis, licorice, flexuose bittercress herb, Chinese date honey, rape pollen and vinegar egg-juice. According to the invention, the components of the additive have medicinal and edible effects, can resist bacteria and diminish inflammation, improve the immunity of banana prawn, also can effectively treat monodon baculovirus disease. Banana prawns eating the additive provided by the invention has strong disease resistance, fast growth speed, and rich meat nutrition, thus greatly increasing the economic benefits of farmers. The additive has the advantages of simple, scientific and reasonable formula, pure natural, green and no residue, strong practicability, no toxic or side effect, and significant effect.

Description

A kind of Penaeus merguiensis de man. feed additive and preparation method thereof

Technical field

The present invention relates to field of feed additive technology, particularly to a kind of Penaeus merguiensis de man. feed additive and preparation side Method.

Background technology

Penaeus merguiensis de man. is commonly called as prawn, shrimp, yellow shrimp, big white shrimp, big shrimp, and edible shrimp, is Crustaceans, with Eriocheir sinensis and Lobster is correlated with.There is flat and resilient translucent health in centre, there is good nutritive value.Penaeus merguiensis de man. nutrition is rich Richness, and its meat is soft, easy to digest, to physical weakness and to need the people taken good care of after being ill be fabulous food；In Penaeus merguiensis de man. Containing abundant magnesium, magnesium has important regulation effect to cardiomotility, can well protect cardiovascular system, and it can reduce blood Cholesterol level in liquid, prevents arteriosclerosis, simultaneously can also coronary artery dilator, beneficially prophylaxis of hypertension and myocardial infarction； The lactogenesis effect of Penaeus merguiensis de man. is relatively strong, and rich in phosphorus, calcium, children's, anemia of pregnant woman are had tonification effect especially；

The delicious meat of Penaeus merguiensis de man. is tender, is of high nutritive value, it has also become one of aquatic products that people are best.The shaft-like disease of Penaeus monodon Viral disease, is the epidemic infectious diseases causing various prawn sick.Baculovirus Diseases of Penaeus monodon mainly encroaches on the hepatopancrease of prawn With promesenteron epithelial tissue, and in nucleus, produce multiple oxyphilous circle or the viral inclusion body of ellipse.Symptom: children Body easy to hang dirty, cultivation shrimp body colour is dark, self-cleaning behavior reduces, torpescence；Loss of appetite, the speed of growth slows down, and shrimp body is thin and weak, liver Atrophy bleaches.Larval stage is injured seriously, mortality rate more than 80%.The feedstuff of commercial type is all general shrimp feed at present, Do not treat the feedstuff of the Baculovirus Diseases of Penaeus monodon of Penaeus merguiensis de man., more do not strengthen the feedstuff of its immunity.

Summary of the invention

The technical problem to be solved is, it is provided that a kind of Penaeus merguiensis de man. feed additive and preparation method, energy Antibacterial, antiinflammatory, improves the immunity of Penaeus merguiensis de man., effectively treats Baculovirus Diseases of Penaeus monodon simultaneously.The edible present invention adds Penaeus merguiensis de man. premunition after agent is strong, and fast growth, meat are nutritious, and the economic benefit of raiser has been significantly greatly increased, tool Have that formula is simple, science, rationally, fully natural green noresidue, practical, have no side effect, the significant advantage of effect.

For solving above-mentioned technical problem, the present invention provides a kind of Penaeus merguiensis de man. feed additive, described additive include with Lower component: Giant salamander oil, Herba Portulacae, Fructus Chaenomelis, Herba Taraxaci, Fructus Luffae, Pericarpium Citri Reticulatae, Bulbus Lilii, Lentinus Edodes, Herba Menthae, Folium Mori, Fructus actinidiae chinensis, Herba Epilobii Pyrricholophi, Canis familiaris L. ant grass, Radix Glycyrrhizae, flexuose bittercress herb and seed, Mel Jujubae, Pollen Brassicae campestris and vinegar-egg juice.

In described additive, the parts by weight of each raw material are: Giant salamander oil 0.5-1.0 part, Herba Portulacae 4-15 part, Fructus Chaenomelis 10-19 Part, Herba Taraxaci 12-22 part, Fructus Luffae 4-11 part, Pericarpium Citri Reticulatae 15-28 part, Bulbus Lilii 8-14 part, Lentinus Edodes 5-16 part, Herba Menthae 9-19 part, macaque Fructus Persicae 3-14 part, Herba Epilobii Pyrricholophi 0.4-1.0 part, Canis familiaris L. ant grass 0.2-0.9 part, Radix Glycyrrhizae 4-12 part, flexuose bittercress herb and seed 0.4-1.2 part, Mel Jujubae 17- 31 parts, Pollen Brassicae campestris 10-23 part and vinegar-egg juice 7-14 part.

Giant salamander oil 0.6-0.8 part, Herba Portulacae 8-11 part, Fructus Chaenomelis 13-17 part, Herba Taraxaci 15-20 part, Fructus Luffae 5-9 part, Pericarpium Citri Reticulatae 18-22 part, Bulbus Lilii 10-12 part, Lentinus Edodes 8-13 part, Herba Menthae 11-16 part, Fructus actinidiae chinensis 6-11 part, Herba Epilobii Pyrricholophi 0.4-0.9 part, Canis familiaris L. ant Grass 0.2-0.7 part, Radix Glycyrrhizae 4-11 part, flexuose bittercress herb and seed 0.4-1.0 part, Mel Jujubae 20-27 part, Pollen Brassicae campestris 14-19 part and vinegar-egg juice 9-11 part.

Giant salamander oil 0.8 part, Herba Portulacae 8 parts, Fructus Chaenomelis 15 parts, Herba Taraxaci 20 parts, 5 parts of Fructus Luffae, Pericarpium Citri Reticulatae 22 parts, Bulbus Lilii 10 parts, 8 parts of Lentinus Edodes, Herba Menthae 16 parts, Fructus actinidiae chinensis 8 parts, Herba Epilobii Pyrricholophi 0.4 part, Canis familiaris L. ant grass 0.7 part, 9 parts of Radix Glycyrrhizae, flexuose bittercress herb and seed 0.8 part, Mel Jujubae 27 parts, Pollen Brassicae campestris 14 parts and vinegar-egg juice 11 parts.

Giant salamander oil 0.7 part, Herba Portulacae 11 parts, Fructus Chaenomelis 13 parts, Herba Taraxaci 18 parts, 7 parts of Fructus Luffae, Pericarpium Citri Reticulatae 18 parts, Bulbus Lilii 12 parts, 10 parts of Lentinus Edodes, Herba Menthae 14 parts, Fructus actinidiae chinensis 11 parts, Herba Epilobii Pyrricholophi 0.9 part, Canis familiaris L. ant grass 0.4 part, 4 parts of Radix Glycyrrhizae, flexuose bittercress herb and seed 1.0 parts, Flos Jujubae Honey 20 parts, Pollen Brassicae campestris 16 parts and vinegar-egg juice 10 parts.

Giant salamander oil 0.6 part, Herba Portulacae 9 parts, Fructus Chaenomelis 17 parts, Herba Taraxaci 15 parts, 9 parts of Fructus Luffae, Pericarpium Citri Reticulatae 20 parts, Bulbus Lilii 11 parts, 13 parts of Lentinus Edodes, Herba Menthae 11 parts, Fructus actinidiae chinensis 6 parts, Herba Epilobii Pyrricholophi 0.6 part, Canis familiaris L. ant grass 0.2 part, 11 parts of Radix Glycyrrhizae, flexuose bittercress herb and seed 0.4 part, Flos Jujubae Honey 23 parts, Pollen Brassicae campestris 19 parts and vinegar-egg juice 9 parts.

For solving above-mentioned technical problem, the present invention also provides for the preparation method of a kind of Penaeus merguiensis de man. feed additive, its system Preparation Method comprises the following steps:

(1) fatty tissue in Andrias davidianus Blanchard body is taken, chopping, clean up, add the deionization of adipose tissue mass 5-7 times Water, after intense fire boils, after change slow fire and boil 5-6h, obtained oil filtrating, remove slag, centrifugal, take supernatant fluid, obtain Andrias davidianus Blanchard Oil；

(2) take egg several, add 6 ° of rice vinegars of egg quality 3 times, seal and soak after 1 week, break seal by Ovum Gallus domesticus album with Egg yolk stirs evenly with vinegar, after continuing to seal 2 days, crosses leaching filtrate and obtains vinegar-egg juice；

(3) Fructus Chaenomelis and Fructus Luffae are put into pulverizer to pulverize, after taking Giant salamander oil, vinegar-egg juice, pulverizing by described mass fraction Fructus Chaenomelis and Fructus Luffae, place 15-18h and obtain mixture one after mix homogeneously；

(4) in described mass fraction ratio take Herba Portulacae, Herba Taraxaci, Pericarpium Citri Reticulatae, Bulbus Lilii, Lentinus Edodes, Herba Menthae, Folium Mori, Fructus actinidiae chinensis, Herba Epilobii Pyrricholophi, Canis familiaris L. ant grass, Radix Glycyrrhizae, flexuose bittercress herb and seed mixing, add relative to the alcohol volumetric concentration of mixture 10～12 times be 90%～ The ethanol of 95%, is heated to boiling reflux 3～5 hours, filters, and collects filtrate, subtracts subsequently under vacuum 0.05～0.08Mpa When pressure is concentrated into 40～50 DEG C, relative density is the mastic of 1.00～1.04, is spray-dried, the inlet temperature 160 of spray dryer ～175 DEG C, leaving air temp 80～85 DEG C, it is ground into powder subsequently, makes dried cream powder；

(5) vinegar-egg juice that described mass parts Mel Jujubae, Pollen Brassicae campestris and step (2) obtain, the dry cream that step (3) obtains are taken Powder mix homogeneously obtains Penaeus merguiensis de man. feed additive.

The technical scheme that the embodiment of the present invention provides has the benefit that its composition is medicinal and edible dual for having concurrently Effect, can antibacterial, antiinflammatory, improve the immunity of Penaeus merguiensis de man., simultaneously effectively treat Baculovirus Diseases of Penaeus monodon.Eat this Penaeus merguiensis de man. premunition after bright additive is strong, and fast growth, meat are nutritious, and the economic effect of raiser has been significantly greatly increased Benefit, has that formula is simple, a science, rationally, and fully natural green noresidue is practical, has no side effect, and effect is the most excellent Point..

Detailed description of the invention

The invention provides a kind of Penaeus merguiensis de man. feed additive and preparation method, have including raw material: Giant salamander oil, Dens Equi Herba Amaranthi tricoloris, Fructus Chaenomelis, Herba Taraxaci, Fructus Luffae, Pericarpium Citri Reticulatae, Bulbus Lilii, Lentinus Edodes, Herba Menthae, Folium Mori, Fructus actinidiae chinensis, Herba Epilobii Pyrricholophi, Canis familiaris L. ant grass, Radix Glycyrrhizae, leucorrhea Grass, Mel Jujubae, Pollen Brassicae campestris and vinegar-egg juice.

Herba Portulacae: another name Dens Equi dish, horse Amaranthus mangostanus L., pig mother's dish, edible kernel of melon seeds dish, Semen Benincasae dish, longevity greens/mustard green, horse Serpentis dish.This product is Portulacaceous plant Herba Portulacae, with all herbal medicine.Acid, cold.Clearing away heat-damp and promoting diuresis, removing pathogenic heat from blood and toxic substance from the body.

Fructus Chaenomelis: another name chaenomeles lagenaria, iron pin pear, wrinkled papaya, Fructus Chaenomelis.This product is the dry of rosaceous plant chaenomeles lagenaria Dry almost ripe fruit.Summer, season in autumn two fruit greenish-yellow time gather, put and boiling water scald to crust canescence, double vertical profile, dry.Acid, Temperature.Return liver, spleen channel.Suppressing the hyperactive liver relaxing muscles and tendons, stomach function regulating removing dampness.

Herba Taraxaci: another name Herba crotalariae albidae, Po Poding.This product is feverfew Herba Taraxaci, alkali ground Herba Taraxaci or belongs to several together The dry herb of plant.Spend spring to autumn and excavate when just opening, remove impurity, clean, dry.Bitter, sweet, cold.Return liver, stomach warp.Clearly Thermal detoxification, dispersing swelling and dissipating binds, inducing diuresis for treating stranguria syndrome.

Fructus Luffae: another name: sky Fructus Luffae, sky sieve, honeydew melon, cloth melon, sky hang melon, pure sun melon, fall sun dish, water melon, wadding melon, fine silk melon, Quite melon, continuous melon.The particular matters such as luffein, lymphatic temperament, wood glue, citrulline, xylan and interferon have certain spy Different effect.The effect have refrigerant, diuresis, invigorating blood circulation, stimulate the menstrual flow, detoxify.

Pericarpium Citri Reticulatae: another name Pericarpium Citri tangerinae.This product is the dry mature skin of rutaceae orange and variety thereof.Medical material is divided into " Pericarpium Citri Reticulatae " and " Pericarpium citri reticuatae chachiensis ".Harvesting ripe fruit, strips peel, dries or cold drying.Bitter, pungent, temperature.Return lung, spleen channel.Regulate the flow of vital energy Spleen invigorating, drying dampness to eliminate phlegm.

Bulbus Lilii: the dry meat scale leaf of Bulbus Lilii or Lilium tenuifolium.Sweet, cold.GUIXIN, lung meridian.Nourishing YIN and moistening the lung, clearing away heart-fire for tranquillization. For deficiency of YIN chronic cough, sputum mixed with blood, fidgets due to deficiency palpitation with fear, insomnia and dreamful sleep, absentminded.

Lentinus Edodes: another name another name: Flammulina velutipes, fragrant bacterium, pawl wild rice, Lentinus Edodes, Lentinus Edodes, fragrant wild rice, fragrant letter.Nature and flavor are sweet, flat, cool；People liver, stomach Warp.There are invigorating the liver and kidney, strengthening the spleen and stomach, replenishing QI and blood, intelligent promoting and the mind tranquilizing, effect of beauty treatment face.

Herba Menthae: this product is the dry aerial parts of Labiatae mint Herba Menthae.Summer, season in autumn two stem and leaf luxuriant or the flowers are in blossom During to three-wheel, select fine day, tap by several times, dry or dry in the shade.Pungent, cool.Return lung, Liver Channel.Dispelling wind-heat.Refresh oneself, rash.

Folium Mori: this product is the dried leaves of moraceae plants Mulberry.Gather after first frost, remove impurity, dry.Sweet, bitter, cold.Return lung, Liver Channel.Dispelling wind and heat pathogens, clearing away lung-heat and moistening for dryness, liver heat removing and eyesight improving.

Fructus actinidiae chinensis: another name Fructus actinidiae chinensis, Fructus Averrhoae Carambolae, white Radix Fici Hirtae, hair pear.Actinidiaceae actinidia Fructus actinidiae chinensis, with root and Fruit medicine.Pluck fruit autumn to uproot, using fresh herb or dry.Really: regulating QI of the middle-JIAO, promote the production of body fluid and moisturize, antipyretic relieving restlessness.Root, root bark: heat clearing away solution Poison, promoting blood circulation and detumescence, expelling wind and removing dampness.

Herba Epilobii Pyrricholophi: another name Japan LIUYECAI, Flos Inulae helianthus-aquaticae are colored, little to through grass, Herba Cynanchi Hancockiani, Herba Stachydis Japonicae, Chrysanthemum carinatum Schousb. pin.For LIUYECAI The Herb of section plant long seed LIUYECAI.Bitter in the mouth；Pungent；Cool in nature.Clearing away heat-damp and promoting diuresis；Arresting bleeding and miscarriage prevention；Removing toxic substances and promoting subsidence of swelling.Main dysentery；Spit blood；Cough Blood；Just hematocele；Menorrhagia；Frequent fetal movement；Ulcerative carbuncle furuncle and phyma；Scald；Traumatic injury wound is swollen；Traumatic hemorrhage.

Canis familiaris L. ant grass: call chain folder bean, practice folder bean, small size Herba cassiae torae, mountain flower life.Pulse family Canis familiaris L. ant grass, enters with herb, root, leaf Medicine.Sweet in the mouth, hardship, property is put down.Promoting blood circulation to remove obstruction in the collateral, clearing away heat and eliminating dampness, refute atrophic debility of bones and swell, removing the necrotic tissue and promoting granulation.

Radix Glycyrrhizae: sweet, flat.GUIXIN, lung, spleen, stomach warp.Invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, expelling phlegm for arresting cough, relieving spasm to stop pain, it is in harmonious proportion all Medicine.For weakness of the spleen and stomach, fatigue and weakness, shortness of breath and palpitation, cough with copious phlegm, gastral cavity abdomen, extremity contraction urgency pain, carbuncle sore tumefacting virus, abirritant Thing toxicity, strong.

Flexuose bittercress herb and seed: another name passeris montani saturati dish, wild foster dish, popped rice perfume (or spice) Herba Capsellae.For crucifer Hairy Bittercress and bending Hairy Bittercress Herb.Sweet in the mouth；Light；Cool in nature.Clearing away heat-damp and promoting diuresis；Calm the nerves；Hemostasis.Main damp-heat dysentery；Pyretic stranguria；Leucorrhea；Cardiopalmus；Insomnia；Asthenic fire tooth Bitterly；Infantile malnutrition；Spit blood；Have blood in stool；Furuncle.

Mel Jujubae: include abundant glucose, fructose and multivitamin and mineral.Property flat temperature partially. function invigorating middle warmer benefit Gas, nourishing blood to tranquillize the mind, protect spleen nourishing the stomach.

Embodiment is below used to describe embodiments of the present invention in detail, whereby to the present invention how application technology means Solve technical problem, and the process that realizes reaching technique effect can fully understand and implement according to this.

Embodiment 1 additive 1

A kind of Penaeus merguiensis de man. feed additive, wherein additive includes: Giant salamander oil 0.8g, Herba Portulacae 8g, Fructus Chaenomelis 15g, Pu Herba Taraxaci 20g, Fructus Luffae 5g, Pericarpium Citri Reticulatae 22g, Bulbus Lilii 10g, Lentinus Edodes 8g, Herba Menthae 16g, Fructus actinidiae chinensis 8g, Herba Epilobii Pyrricholophi 0.4g, Canis familiaris L. ant grass 0.7g, Radix Glycyrrhizae 9g, flexuose bittercress herb and seed 0.8g, Mel Jujubae 27g, Pollen Brassicae campestris 14g and vinegar-egg juice 11g.

The preparation method of additive comprises the following steps:

(1) fatty tissue in Andrias davidianus Blanchard body is taken, chopping, clean up, add the deionized water of adipose tissue mass 6 times, After intense fire boils, after change slow fire and boil 5h, obtained oil filtrating, remove slag, centrifugal, take supernatant fluid, obtain Giant salamander oil；

(3) Fructus Chaenomelis and Fructus Luffae are put into pulverizer to pulverize, after taking Giant salamander oil, vinegar-egg juice, pulverizing by described mass fraction Fructus Chaenomelis and Fructus Luffae, place 17h and obtain mixture one after mix homogeneously；

(5) taking the mixture one that described mass parts Mel Jujubae, Pollen Brassicae campestris and step (3) obtain, it is dry that step (4) obtains Cream powder mix homogeneously obtains Penaeus merguiensis de man. feed additive.

Embodiment 2 additive 2

A kind of Penaeus merguiensis de man. feed additive, wherein additive includes: Giant salamander oil 0.7g, Herba Portulacae 11g, Fructus Chaenomelis 13g, Pu Herba Taraxaci 18g, Fructus Luffae 7g, Pericarpium Citri Reticulatae 18g, Bulbus Lilii 12g, Lentinus Edodes 10g, Herba Menthae 14g, Fructus actinidiae chinensis 11g, Herba Epilobii Pyrricholophi 0.9g, Canis familiaris L. ant grass 0.4g, Radix Glycyrrhizae 4g, flexuose bittercress herb and seed 1.0g, Mel Jujubae 20g, Pollen Brassicae campestris 16g and vinegar-egg juice 10g.

The preparation method of additive comprises the following steps:

(1) fatty tissue in Andrias davidianus Blanchard body is taken, chopping, clean up, add the deionized water of adipose tissue mass 7 times, After intense fire boils, after change slow fire and boil 6h, obtained oil filtrating, remove slag, centrifugal, take supernatant fluid, obtain Giant salamander oil；

(3) Fructus Chaenomelis and Fructus Luffae are put into pulverizer to pulverize, after taking Giant salamander oil, vinegar-egg juice, pulverizing by described mass fraction Fructus Chaenomelis and Fructus Luffae, place 15h and obtain mixture one after mix homogeneously；

Embodiment 3 additive 3

A kind of Penaeus merguiensis de man. feed additive, wherein additive includes: Giant salamander oil 0.6g, Herba Portulacae 9g, Fructus Chaenomelis 17g, Pu Herba Taraxaci 15g, Fructus Luffae 9g, Pericarpium Citri Reticulatae 20g, Bulbus Lilii 11g, Lentinus Edodes 13g, Herba Menthae 11g, Fructus actinidiae chinensis 6g, Herba Epilobii Pyrricholophi 0.6g, Canis familiaris L. ant grass 0.2g, Radix Glycyrrhizae 11g, flexuose bittercress herb and seed 0.4g, Mel Jujubae 23g, Pollen Brassicae campestris 19g and vinegar-egg juice 9g.

The preparation method of additive comprises the following steps:

(1) fatty tissue in Andrias davidianus Blanchard body is taken, chopping, clean up, add the deionized water of adipose tissue mass 5 times, After intense fire boils, after change slow fire and boil 5h, obtained oil filtrating, remove slag, centrifugal, take supernatant fluid, obtain Giant salamander oil；

(3) Fructus Chaenomelis and Fructus Luffae are put into pulverizer to pulverize, after taking Giant salamander oil, vinegar-egg juice, pulverizing by described mass fraction Fructus Chaenomelis and Fructus Luffae, place 18h and obtain mixture one after mix homogeneously；

Embodiment 4 additive 4

A kind of Penaeus merguiensis de man. feed additive, wherein additive includes: Herba Portulacae 8g, Fructus Chaenomelis 15g, Herba Taraxaci 20g, Fructus Luffae 5g, Pericarpium Citri Reticulatae 22g, Bulbus Lilii 10g, Lentinus Edodes 8g, Herba Menthae 16g, Fructus actinidiae chinensis 8g, Herba Epilobii Pyrricholophi 0.4g, Canis familiaris L. ant grass 0.7g, Radix Glycyrrhizae 9g, leucorrhea Grass 0.8g, Mel Jujubae 27g, Pollen Brassicae campestris 14g and vinegar-egg juice 11g.

The preparation method of additive comprises the following steps:

(1) take egg several, add 6 ° of rice vinegars of egg quality 3 times, seal and soak after 1 week, break seal by Ovum Gallus domesticus album with Egg yolk stirs evenly with vinegar, after continuing to seal 2 days, crosses leaching filtrate and obtains vinegar-egg juice；

(2) Fructus Chaenomelis and Fructus Luffae are put into pulverizer to pulverize, the Fructus Chaenomelis after being taken vinegar-egg juice by described mass fraction, pulverized and silk Melon, places 17h and obtains mixture one after mix homogeneously；

(3) in described mass fraction ratio take Herba Portulacae, Herba Taraxaci, Pericarpium Citri Reticulatae, Bulbus Lilii, Lentinus Edodes, Herba Menthae, Folium Mori, Fructus actinidiae chinensis, Herba Epilobii Pyrricholophi, Canis familiaris L. ant grass, Radix Glycyrrhizae, flexuose bittercress herb and seed mixing, add relative to the alcohol volumetric concentration of mixture 10～12 times be 90%～ The ethanol of 95%, is heated to boiling reflux 3～5 hours, filters, and collects filtrate, subtracts subsequently under vacuum 0.05～0.08Mpa When pressure is concentrated into 40～50 DEG C, relative density is the mastic of 1.00～1.04, is spray-dried, the inlet temperature 160 of spray dryer ～175 DEG C, leaving air temp 80～85 DEG C, it is ground into powder subsequently, makes dried cream powder；

(4) taking the mixture one that described mass parts Mel Jujubae, Pollen Brassicae campestris and step (2) obtain, it is dry that step (3) obtains Cream powder mix homogeneously obtains Penaeus merguiensis de man. feed additive.

Embodiment 5 additive 5

A kind of Penaeus merguiensis de man. feed additive, wherein additive includes: Giant salamander oil 0.8g, Herba Portulacae 8g, Fructus Chaenomelis 15g, Pu Herba Taraxaci 20g, Fructus Luffae 5g, Pericarpium Citri Reticulatae 22g, Bulbus Lilii 10g, Lentinus Edodes 8g, Herba Menthae 16g, Fructus actinidiae chinensis 8g, Herba Epilobii Pyrricholophi 0.4g, Canis familiaris L. ant grass 0.7g, Radix Glycyrrhizae 9g, flexuose bittercress herb and seed 0.8g, Mel Jujubae 27g and Pollen Brassicae campestris 14g.

The preparation method of additive comprises the following steps:

(1) fatty tissue in Andrias davidianus Blanchard body is taken, chopping, clean up, add the deionization of adipose tissue mass 5-7 times Water, after intense fire boils, after change slow fire and boil 6h, obtained oil filtrating, remove slag, centrifugal, take supernatant fluid, obtain Andrias davidianus Blanchard Oil；

(2) Fructus Chaenomelis and Fructus Luffae are put into pulverizer to pulverize, the Fructus Chaenomelis after being taken Giant salamander oil by described mass fraction, pulverized and silk Melon, places 17h and obtains mixture one after mix homogeneously；

Embodiment 6 additive 6

A kind of Penaeus merguiensis de man. feed additive, wherein additive includes: Giant salamander oil 0.8g, Herba Portulacae 8g, Fructus Chaenomelis 15g, Pu Herba Taraxaci 20g, Fructus Luffae 5g, Pericarpium Citri Reticulatae 22g, Bulbus Lilii 10g, Lentinus Edodes 8g, Herba Menthae 16g, Fructus actinidiae chinensis 8g and vinegar-egg juice 11g.

The preparation method of additive comprises the following steps:

(4) Herba Portulacae, Herba Taraxaci, Pericarpium Citri Reticulatae, Bulbus Lilii, Lentinus Edodes, Herba Menthae, Fructus actinidiae chinensis mixing are taken in described mass fraction ratio, Add relative to mixture 10～the ethanol that alcohol volumetric concentration is 90%～95% of 12 times, be heated to boiling reflux 3～5 little Time, to filter, collect filtrate, when being evaporated to 40～50 DEG C subsequently under vacuum 0.05～0.08Mpa, relative density is The mastic of 1.00～1.04, is spray-dried, the inlet temperature 160 of spray dryer～175 DEG C, leaving air temp 80～85 DEG C, with After be ground into powder, make dried cream powder；

(5) dried cream powder that the mixture one step (3) obtained obtains with step (4) is mixed homogeneously and is obtained Penaeus merguiensis de man. and raise Feed additives.

Safety testing

1, acute toxicity test

(1) experimental animal, feedstuff and raising

Cleaning grade Kun Ming mice, body weight 18-22g, 20, male and female half and half.Limited by Shanghai Si Laike laboratory animal Company provides.Full nutrition growth feedstuff is provided by Guangzhou Datainong Feed Co., Ltd..II grade of Animal House, saves the most round the clock Rule, temperature: 24 ± 2 DEG C, humidity: 45 ± 5%.

(2) test reagent

Carboxymethyl cellulose (analytical pure), is configured to 0.5% suspension with distilled water stand-by.

(3) test method

Test method selects maximum tolerated dose method.Taking 20 body weight is cleaning grade Kun Ming mice healthy for 18-22g, Each 10 of male and female.Fasting 16h (overnight) before test, can't help water.Take the additive 1 in the 5g embodiment of the present invention 1, use 0.4% carboxylic Methylcellulose is diluted to 18mL.Dividing upper and lower noon every bis-per os gavages of 4h, after gavage, 2h takes food for the second time, total gavage agent Amount is 10g (kg bw)^-1.Continuous Observation 14d, record poisoning manifestations and death condition.

2, mutagenicity test

2.1 test strain

TA97, TA98, TA100, TA102 tetra-strain Salmonella typhimurium.Thered is provided by Center for Disease Control (CDC) of Heilongjiang Province.

2.2 experimental animals and reagent

(1) Salmonella reversion test:

The preparation of nutrient broth medium: Carnis Bovis seu Bubali cream 2.4g, peptone 5.0g, sodium chloride 2.4g, dipotassium hydrogen phosphate (K2HPO4 3H2O) 1.2g, distilled water 500mL.Heating for dissolving, by mentioned reagent, adjusts pH to 7.4, subpackage, sterilizing (0.103MPa, 20min), general refrigerator saves backup (less than half a year).

Phosphate stock solution is prepared: dibastic sodium phosphate ammonia (NaNH4HPO4 4H2O) 17.4g, citric acid (C6H8O7 H2O) 10.0g, dipotassium hydrogen phosphate (K2HPO4) 50.0g, magnesium sulfate (MgSO4 7H2O) 1.0g.

Magnesium sulfate is the most slowly put into after other reagent are completely dissolved so that it is continue to dissolve (otherwise can separate out precipitation).

1.5% agar culture medium preparation: agar Agar6.0g, instillation distilled water to 400mL, after mentioned reagent is melted 0.102MPa sterilizing 25min.

Bottom culture medium is prepared: sterilizing agar culture medium (80 DEG C) 400mL, phosphate stock solution 7mL, 40% glucose is molten Liquid 20mL, is sequentially added into mentioned reagent in a reservoir, fully mixes, and is down flat ware when temperature is down to about 80 DEG C, every ware 25mL, Remove moisture while 37 DEG C of overnight incubation, then check for polluting.

Top agar is prepared: agar 3.0g, sodium chloride 2.5g, instillation distilled water to 500Ml.

0.5mmol/L histidine-biotin solution preparation: Bio D-Biotin (molecular weight 244) 30.4mg, L-group Propylhomoserin L-Histidine (molecular weight 155) 19.3mg, instillation distilled water to 250mL

The preparation of 10%S-9 mixed liquor: phosphate buffer (0.2mol/L, pH7.4) 6mL, Klorvess Liquid (1.65mol/L) 0.2mL, magnesium chloride solution (0.4mol/L) 0.2mL, G6Pna saline solution (0.05mol/L) 1.0mL, coenzyme-II solution (0.0025mol/L) 1.5mL, liver S-9 liquid 1.0mL mix, and face used time preparation, put in water-bath stand-by.

Liver S-9 liquid is provided by Center for Disease Control (CDC) of Heilongjiang Province.Standard mutagenesis agent is respectively fenaminosulf, sodium azide, 2-second Acylamino-fluorenes, 1,8-istizin, Center for Disease Control (CDC) of Heilongjiang Province provide.

(2) micronucleus test

50 cleaning grade Kun Ming mice, body weight 25-30g, male and female half and half.By the Shanghai limited public affairs of Si Laike laboratory animal Department provides.Full nutrition growth feedstuff is provided by Guangzhou Datainong Feed Co., Ltd..II grade of Animal House, artificial circadian rhythm, Temperature: 24 ± 2 DEG C, humidity: 45 ± 5%.

Calf serum: calf serum is put in water bath with thermostatic control after considering bacterium, 56 DEG C of inactivation 1h.4 DEG C it are stored at after inactivation Refrigerator saves backup.

Jim Sa (Giemsa) dye liquor: weigh Giemsa3.8g, adds 375ml methanol (analytical pure) and grinds, treat the most molten Xie Hou, adds 125ml glycerol.It is placed in 37 DEG C of calorstat 48h to shake for several times.Filter, stand two Zhou Houyong.

Jim Sa (Giemsa) application liquid: take a Giemsa dye liquor and 6 parts of phosphate buffers mix.Face the used time Preparation.

1/15moL/L phosphate buffer (pH6.8) is prepared: potassium dihydrogen phosphate (KH2PO4) 4.50g, sodium dihydrogen phosphate (Na2HPO4 12H2O) 11.78g, instillation distilled water is to 1000mL, and whole reagent, in addition to indicating, are analytical pure, and test is used Water is distilled water.

(3) sperm malformation test

50 cleaning grade Kunming kind male white mouses, body weight 25-35g.Carried by Shanghai Slac Experimental Animal Co., Ltd. Supply.Full nutrition growth feedstuff is provided by Guangzhou Datainong Feed Co., Ltd..Artificial circadian rhythm, temperature: 24 ± 2 DEG C, wet Degree: 45 ± 5%.

Methanol.1%～2% eosin stains liquid: weigh Yihong 1～2g, be dissolved in 100mL distilled water standby.All reagent removes Outside indicating, being analytical pure, test water is distilled water.

2.3 test method

2.3.1Ames test

(1) principle: saltant type (i.e. histidine deficient) bacterial strain of Salmonella typhimurium is under the conditions of having histidine Can not be able to grow in the culture medium existed without histidine with normal growth in culture medium.If but having mutagen to be present in nothing Time in the culture medium of histidine, then mutant salmonella type can back mutation be wild type (Phenotype), thus can be grown on Without in the culture medium of histidine, so can be that standard judges that tested material causes the power of prominent property according to bacterium colony quantity of formation.Some It is wild type that special tested material needs just to make mutant salmonella type back mutation after metabolism activation system processes, generation Thank to activation system and use S-9 mixed liquor (preparation method: utilize Polychlorinated biphenyls (Polychlorinated biphenyl, PCB) to lure Lead rat liver homogenate (S-9)).

(2) test strain: use TA97, TA98, TA100, TA102 tetra-strain Salmonella typhimurium saltant, TA97 and TA98 can detect various frame shift type mutagenic agent；TA100 can detect the mutagenic agent causing base pair replacement；TA102 energy Detect some mutagenic agent that other test strain can not detect or seldom detect.

Additive 1 dosage in (3) 5 embodiment of the present invention 1 is respectively 5000,1000,200,40 and 8 μ g/ wares.

(4) increase bacterium training and take nutrient broth medium 5ml, add in aseptic little triangular flask or sterile test tube, freezing is protected The inoculation deposited is in nutrient broth medium, and 37 DEG C of vibrations (100 times/min) cultivate 10h to increased logarithmic phase, every milliliter Viable count is more than 1 × 10⁹～2 × 10⁹Individual, wrap up in culture bottle with black paper bag, irradiated by light with bacteriological protection.

(5) several bottom culture medium plate is prepared.

(6) top layer culture medium melted and be sub-packed in aseptic small test tube, often pipe 2ml, being incubated in 45 DEG C of water-baths.

(7) fresh for the test strain of 0.1ml enrichment liquid is sequentially added in the top layer culture medium of insulation, mixing；Then drip Enter 0.1ml tested material (separately adding 0.5ml 10%S-9 mixed liquor during activation), then mix, be quickly poured in bottom culture medium, and Making it be uniformly distributed on bottom, keep flat solidification, in sterile board, (37 DEG C) cultivate 48h observed result.

(8) separately do solvent control (i.e. negative control, distilled water 0.1ml/ ware) and positive control (is respectively adopted sodium azide 1.5 μ g/ wares, fenaminosulf 50 μ g/ ware, 2-acetamidofluorene 10 μ g/ ware, 1,8-istizin 50 μ g/ ware).Solvent control adds goes out Bacterium distilled water；Positive control is not added with tested material, only adds standard mutagenic agent；Additive method is ibid.It is repeated twice.

2.3.2 micronucleus test

Table 1 micronucleus test design (n=10)

Dosage group	Dosage (g (kg bw d)^-1)
		Negative group	0.5% carboxymethyl cellulose
Low dose group	0.5
		Middle dosage group	5
High dose group	9
		Positive group	0.04

It is administered to tested material method by 30h, positive controls cycli phosphate amine once abdominal cavity injection 0.04g (kg bw)^-1, Remaining respectively organizes gavage, 2 dosing interval 24h, and after being administered for the 2nd time, 6h puts to death animal.

Film-making: after being administered 6h at the 2nd time, mice is taken off cervical vertebra and puts to death, take mice vertebra, remove muscle, cut off the bone of one end Vertebra, drips in calf serum (0.05mL is placed on microscope slide) with needle-nose pliers extrusion bone marrow.

Push jack: after mixing, push jack several, standing and drying.

Fixing: with methanol solution, dry smear being fixed 5～10min, taking-up is dried.The same day, achromophil smear also should Preserve after Gu Ding.

Dyeing: the smear 15～30min fixed with Jim Sa-phosphate buffer (pH is 6.4) dyeing of 1:10.With steaming Distilled water is rinsed, the most to be checked.Every white mice counts 1 000 PCE (Poiychromatic erythrocytes, PCE), Micronuclear rates represents with ‰；It addition, when counting PCE, count RBC (Red blood cell count, RBC) number, calculate simultaneously PCE/RBC value.

2.3.3 sperm malformation test

Table 2 sperm malformation test design (n=10)

Dosage group	Dosage (g (kg bw d)^-1)
		Negative group	0.5% carboxymethyl cellulose
Low dose group	0.5
		Middle dosage group	5
High dose group	10
		Positive group	0.04

Every day contaminates once, and continuous 5d records body weight, food-intake and absorption additive capacity every day.Positive controls uses 0.04g·(kg·bw·d)^-1Cyclophosphamide carries out lumbar injection, and the administering mode of the embodiment of the present invention 1 additive 1 is gavage. Require during off-test often to organize at least 5 surviving animals.

Sperm sampling and microscopy: after giving tested material first, mice cervical dislocation is put to death by the 5th week (35d), opens Abdominal cavity, wins both sides epididymis, puts in little plate and (fills about 2ml normal saline).Being shredded with eye scissors by epididymis (can not be too Broken).With four layers, fragment of tissue is wiped paper filter off.Filtrate is centrifuged 5min (1000～1500r/min).Retain about 0.5ml liquid, its Remaining supernatant reject, after itself and precipitate being shaken up, drips 1 on clean microscope slide and carries out smear (typically every mice is cooked 4 ～5 smears).Smear is dried in atmosphere, uses methanol solution to fix 5min.With 2% Yihong solution at its natural drying Poststaining 1h, gently rushes with water, is dried to be checked.

Under low power lens, find that sperm overlap is less, the part of clear background, with high power lens sequential search sperm, and count Number.All imperfect, profile is unclear, or overlapping, or obvious genus does not artificially shred person and do not calculates.One sperm is only counted the brightest Aobvious a kind of deformity.

Secondly the deformity of sperm is mainly manifested in head, and at afterbody, deformity type has banana-shaped, amorphous, Wugou, fat Head, double end, double tail, tail folding etc..The sperm count of recording exceptional and Exception Type, and calculate the sperm composition ratio of deformity type. Every 1000 complete sperms of Mus meter, abnormal rate represents with ‰.

Rate of teratosperm (‰)=sperm deformity sum/check sperm sum × 1 000

3, chronic and subchronic test

3.1 experimental animals, feedstuff and raising

3.1.1 rat 30d feeding trial

Cleaning grade Wistar rat, body weight 150-180g, 80, male and female half and half.Had by Shanghai Si Laike laboratory animal Limit company provides.Full nutrition growth feedstuff by Beijing Australia pull together feed corporation,Ltd provide.II grade of Animal House, saves the most round the clock Rule, temperature: 24 ± 2 DEG C, humidity: 45 ± 5%.

3.1.2 laying hen 56d feeding trial

29 week old sea match laying hens 240, are provided by Harbin benefit agriculture fowl industry.Basic drawing is reached feed factory by Harbin China and carries Supply.Layer breeding agriculture university's practice base Laying House northeastward.

3.2 test reagent

General chemistry reagent: formaldehyde, paraffin, ethanol, dimethylbenzene, hematoxylin, Yihong, acetone, phosphate buffer etc..

Biochemical Indexes test kit: glutamic oxaloacetic transaminase, GOT (Glutamic-oxalacetic transaminease, GOT), Glutamate pyruvate transaminase (Glutamic-pyruvic transaminase, GPT), blood urea nitrogen (Urea nitrogen, BUN), creatinine (Creatinine, CRE), cholesterol (Cholesterol, CHO), blood glucose (Glucose, GLU), TG, albumin (Albumin, ALB), total protein (Total protein, TP) test kit is purchased from middle raw north control (product batch number: 100731)

Cellanalyzer test agent: stain (STROMATOLYSER-4DS FFS-800A), haemproteins detection examination Agent (SULFOLYSERSLS-210A1015), basophilic granulocyte and white blood cell detection reagent (STROMATOLYSER- FBR1039), diluent (PK-30L G2109).All reagent is analytical pure, and test water is distilled water.

3.3 test method

3.3.1 rat 30d feeding trial

Table 3 rat 30d feeding trial design (n=20)

Group	Process
		Matched group	Complete feed
Low dose group	Complete feed+0.4% embodiment of the present invention 1 additive 1
		Middle dosage group	Complete feed+2% embodiment of the present invention 1 additive 1
High dose group	Complete feed+10% embodiment of the present invention 1 additive 1

Animal is bought after isolation is fed 1 week and is for experiment, often group male and female half and half.Experimental animal free choice feeding, freely drink water.

(1) physiochemical indice:

General index: observe the searching for food of rat, drink water, fall ill and the situation such as death, every day twice sooner or later, body weight with raise Material is each weekly to be claimed once, and calculates average weight gain, feed intake, starting weight, end weight and efficiency of feed utilization.

Hematological indices: 30d feeds after terminating, blood sampling sample, mensuration erythrocyte (Red blood cell count, RBC), leukocyte (White blood cell count, WBC), hemoglobin (Hemoglobin, HGB), lymphocyte number (Lymphocyte, LYM) and leukocyte differential count.

Biochemical indicator: 30d feed terminate after, gather Rat blood samples centrifuging and taking serum, measure GOT, GPT, BUN, CRE、CHO、GLU、TG、ALB、TP。

(2) pathological examination:

Postmortem: observing and record the naked eyes change of each system organ, tissue, typical cytopathic is taken pictures.

Organ coefficient: the tissues such as heart, liver, kidney, spleen, testis or ovary and body weight are weighed, and calculate internal organs system Number.

Histological examination: while above-mentioned Dissection test animal changes with perusal, core dirty, kidney, spleen, liver The organs and tissues 1 such as dirty, stomach, testis or ovary～2 pieces, fix with formalin, paraffin embedding, section, HE (H Ematine, HE) dyeing, basis of microscopic observation record organization change.

3.3.2 laying hen 56d feeding trial

Table 4 laying hen 56d feeding trial design (n=60)

Group	Process
		Matched group	Basic drawing
Low dose group	Basic drawing+0.4% embodiment of the present invention 1 additive 1
		Middle dosage group	Basic drawing+2% embodiment of the present invention 1 additive 1
High dose group	Basic drawing+10% embodiment of the present invention 1 additive 1

Animal starts test after adapting to 7 days under this experimental condition after buying again.Experimental animal free choice feeding, freely drink Water.

(1) physiochemical indice:

General index: observe the searching for food of laying hen, drink water, fall ill and the situation such as death, every day twice sooner or later, to egg size with Feedstuff respectively claims once every day, and calculates feedstuff-egg ratio, laying rate.

Hematological indices: after nursing in 56 days terminates, carries out blood sampling and measures RBC, WBC, HGB, LYM laying hen.

Biochemical indicator: 56 days feed terminate after, laying hen is acquired centrifugal blood take determination of serum GOT, GPT, BUN、CRE、CHO、GLU、TG、ALB、TP。

(2) pathological examination:

Postmortem: perusal also records the change of each system organ, tissue, and typical cytopathic is taken pictures.

Organ coefficient: the tissue weight such as satisfactory dirty, liver, lungs, kidney, spleen, pancreas, glandular stomach, muscular stomach and body weight.

Histological examination: while above-mentioned Dissection test animal changes with perusal, take liver, spleen, kidney, gland The organs and tissues such as stomach 1～2 pieces are fixed with formalin, paraffin embedding, section, and HE dyes, basis of microscopic observation record organization Learn change.

3.4 data process

Result mean+SD represents, uses SPSS 17.0 to carry out statistical analysis.Acute toxicity test, DABAI Mus 30d feeding trial and laying hen 56d feeding trial result carry out single factor test variance (one-wayANOVA) and analyze；Salmonella reversion test is tied Fruit and mouse marrow cell micro nuclear test result all carry out X 2 test；Mouse inbred strain result carries out single factor test variance Analyze and Duncan ' s method multiple comparisons.P < 0.05 is significance of difference criterion, and P < 0.01 significantly judges mark for difference Accurate.

4, result and analysis

4.1 acute toxicity

Table 5 acute toxicity

From table 5, after off-test, all mices are the most without exception during testing, drink water, search for food and feces the most just Often, all survive；The weight gain of two kinds of sex mices has no significant difference (P > 0.05).By whole mices after off-test De-neck is put to death, and dissects, and the internal organs such as the perusal heart, liver, spleen, lung, stomach, kidney, thymus all do not occur that abnormal pathologic changes.Result table Bright, the LD50 > 10mg (kg bw)-1 of the embodiment of the present invention 1 additive 1, belong to actual non-toxic type material.

4.2 mutagenicity

4.2.1Ames test

From table 6,7,8,9, compared with negative control group, in the case of adding and being not added with S-9, twice Salmonella reversion test is positive Matched group recovery mutation colony number significantly increases (P < 0.01), the embodiment of the present invention 1 additive 1 each dosage group back mutation bacterium Fall number without significant difference (P > 0.05), without dose-response relationship, i.e. under this dosage the embodiment of the present invention 1 additive 1 to mouse typhus Salmonella is that mutagenesis is negative.

Table 6 embodiment of the present invention 1 additive 1 impact (test ,-S9 for the first time) on recovery mutation colony number

Note: in same column, * represents that difference is extremely notable (P < 0.01).Table 7,8,9 is same

Table 7 embodiment of the present invention 1 additive 1 impact (test ,+S9 for the first time) on recovery mutation colony number

Table 8 embodiment of the present invention 1 additive 1 impact (second time test ,-S9) on recovery mutation colony number

Table 9 embodiment of the present invention 1 additive 1 impact (second time test ,+S9) on recovery mutation colony number

4.2.2 mouse marrow cell micro nuclear test

Table 10 mouse marrow cell micro nuclear test (n=10)

Note: in same column, shoulder mark * person represents that difference is extremely notable (P < 0.01)

From table 10, compared with negative control group, positive controls micronuclear rates pole significantly improves (P < 0.01), female, male The mice embodiment of the present invention 1 additive 1 respectively organizes micronuclear rates all without significant difference (P > 0.05), illustrates that the present invention is real under this dosage Execute example 1 additive 1 not make significant difference Micronucleus, i.e. this result of the test is negative.Additionally, except positive control Outside group, the embodiment of the present invention 1 additive 1 each group PCE/RBC compared with negative control group, without significant difference (P > 0.05), illustrates this Under dosage, the embodiment of the present invention 1 additive 1 has no cytotoxic effect to experimental animal.

4.2.3 mouse inbred strain

From table 11, each group mice all has a number of teratospermia to occur, compared with negative control group, positive right Significantly improve (P<0.01) according to group rate of teratosperm pole, the embodiment of the present invention 1 additive 1 respectively organize difference not significantly (P> 0.05), without dose-response relationship, illustrate that Sperm Abnormalities of Mice is not had by the embodiment of the present invention 1 additive 1 under this dosage Have an impact.

Table 11 mouse inbred strain (n=10)

Group	Sperm deformity number (individual)	Rate of teratosperm (‰)
			Negative group	27	5.4
Low dose group	27	5.4
			Middle dosage group	35	7.0
High dose group	42	8.4
			Positive group	511^*	102.2^*

4.3 is chronic chronic with Asia

4.3.1 rat 30d feeding trial

4.3.1.1 general index

Table 12 rat 30d feeding trial weight gain, food-intake, food utilization (n=10)

From table 12, weight gain, total food-intake and food after the embodiment of the present invention 1 additive 1 feed rat 30d Utilization rate compared with matched group all without significant difference (P > 0.05).

4.3.1.2 hematological indices

Table 13 rat 30d feeding trial hematological indices (1) (n=10)

Table 14 rat 30d feeding trial hematological indices (2) (n=10)

From table 13 and 14, compared with matched group, the embodiment of the present invention 1 additive 1 feeds male and female rat after rat 30d Leukocyte, erythrocyte, hemoglobin and differential blood count difference the most notable (P > 0.05).

4.3.1.3 blood parameters

Table 15 rat 30d feeding trial blood parameters (1) (n=10)

Table 16 rat 30d feeding trial blood parameters (2) (n=10)

From table 15 and 16, compared with matched group, the embodiment of the present invention 1 additive 1 feeds male and female rat after rat 30d 9 blood parameters differences the most notable (P > 0.05).

4.3.1.4 pathological examination

(1) gross examination of skeletal muscle

Dissect each group of rat after off-test and carry out gross necropsy.Respectively organize rat trachea, heart, thoracic aorta, liver, kidney, Adrenal gland, spleen, stomach, caecum, colon, rectum, pancreas, part mesenteric lymph node, mammary gland, duodenum, jejunum, prostate, The tissue such as testis, epididymis seminal vesicle or ovary, uterus, vagina, sciatic nerve, bladder, skin is showed no obvious abnormalities.

Table 17 rat 30d feeding trial organ weight ratio (1) (n=10)

Table 18 rat 30d feeding trial organ weight ratio (2) (n=10)

From table 17,18, the embodiment of the present invention 1 additive 1 feeds the internal organs after rat 30d with matched group male and female rat It is the most not notable (P > 0.05) that weight ratio compares difference.

(2) organs and tissues pathologic finding

After off-test, all rat hearts, liver, spleen, kidney, stomach, testis or ovary are carried out HE dyeing.Under microscope Observing, each dosage group has no any obvious exception compared with matched group

4.3.2 laying hen 56d feeding trial

4.3.2.1 general index

From table 19, compared with matched group, laying hen after the 56d embodiment of the present invention 1 additive 1 feeds daily ingestion amount, Average egg weight difference is not the most notable (P > 0.05)；Average egg production significantly improves, and feedstuff-egg ratio significantly reduces (P < 0.05), shows to fit When the dosage embodiment of the present invention 1 additive 1 can improve the production performance of laying hen.

Table 19 laying hen feeding trial generality index (n=60)

Note: in colleague, shoulder upper letter difference person represents significant difference (P < 0.05)

4.3.2.2 hematological indices

From table 20, compared with matched group, laying hen leukocyte, red after the 56d embodiment of the present invention 1 additive 1 feeds Cell, hemoglobin, hematocrit value, mean corpuscular volume, mean corpuscular hematochrome, erythrocyte average protein concentration, Platelet difference is not the most notable (P > 0.05).

Table 20 laying hen feeding trial hematological indices (n=60)

4.3.2.3 blood parameters

Table 21 laying hen feeding trial blood parameters (n=60)

Note: in colleague, shoulder upper mark * person represents significant difference (P < 0.05)

From table 21, compared with matched group, high dose group laying hen blood BUN level significantly reduces (P < 0.05), other Index is all without significant difference (P > 0.05).

4.3.2.4 pathological examination

(1) gross examination of skeletal muscle

After off-test, each group of laying hen is dissected, carries out gross necropsy.Each group main organs trachea of laying hen, heart, Liver, kidney, adrenal gland, spleen, stomach, caecum, colon, rectum, pancreas, part mesenteric lymph node, duodenum, ovary, uterus, skin Skins etc. by the naked eye, show no obvious abnormalities.

Table 22 laying hen feeding trial organ weight ratio (n=60)

From table 22, compared with matched group, the embodiment of the present invention 1 additive 1 respectively organizes laying hen internal organs weight differences the most not Significantly (P > 0.05).

(2) organs and tissues pathologic finding

Liver of Laying Hens, spleen, kidney, glandular stomach through HE dye, examine under a microscope, each dosage group compared with matched group the most not See any obvious exception

To sum up experimental result:

1. the median lethal dose(LD 50) of the embodiment of the present invention 1 additive 1 is more than 10g (kg bw)-1, belongs to actual non-toxic type Material.

2., under this experimental condition, the embodiment of the present invention 1 additive 1 does not has mutagenicity.

3., under this experimental condition, the embodiment of the present invention 1 additive 1 does not has chronic and subchronic toxicity to rat and laying hen, And the production performance of laying hen can be improved.

In sum, this result of the test is pointed out, and under this experimental condition, the embodiment of the present invention 1 additive 1 had not both had acute poison Property, mutagenicity, do not have chronic or subchronic toxicity yet, and the embodiment of the present invention 1 additive 1 is the most permissible under suitable dosage As a kind of green, natural, safe functional feedstuff additive, and it is applied in husbandry sector.

On the additive of embodiments of the invention 2, embodiment 3, embodiment 4, embodiment 5 and embodiment 6 has been carried out equally State test, be also demonstrated that the additive of the embodiment of the present invention 2, embodiment 3, embodiment 4, embodiment 5 and embodiment 6 is both the most anxious Property toxicity, mutagenicity, there is no chronic or subchronic toxicity yet, completely can be as a kind of green, sky under suitable dosage So, the functional feedstuff additive of safety, and be applied in husbandry sector.

Contrast test:

Choose certain Penaeus merguiensis de man. plant.Select that outward appearance is normal, body constitution is healthy and strong, Penaeus merguiensis de man. of uniform size, by 4200 Tail ink is lucky to being randomly divided into 7 groups, often group 600, and 7 groups respectively feed the embodiment of the present invention 1 additive 1 group, feed the present invention Embodiment 2 additive 2 groups, feed the embodiment of the present invention 3 additive 3 groups, feed the embodiment of the present invention 4 additive 4 groups, feed this Inventive embodiments 5 additive 5 groups, feed the embodiment of the present invention 6 additive 6 groups and feed the contrast groups of normal diet.Additive Quality accounts for the 0.08% of feedstuff gross weight.Being put in a suitable place to breed respectively in the net cage of 3.0m*3.0m*1.0m by 7 groups of Penaeus merguiensis de man.s, test is used Water is that natural sea-water filters gained through sand, and salinity is 31-33, and water temperature is 23 DEG C-26 DEG C, and the depth of water is 0.6m, and process of the test is dissolved Oxygen is maintained at more than 6.0mg/L.Experimental period is 45 days.Daily management increase the DO every day is once.During test, feeding volume is by ink The 7-9% of lucky prawn body weight throws something and feeds, throw raise 3 times/d of frequency (morning 7:00-8:00,15:00-16:00 in afternoon, evening 22: 00-23:00), early, middle and late feeding volume is respectively 35%, 30% and 35%.

1, rate of body weight gain measures

Take 60 tail prawns at random the 1st day tested, the 45th day (raising terminates), weigh the often group long body of prawn body respectively Weight, calculates the long rate of increase of body, body weight increase rate.

The long rate of increase of body (%)=[(test opisthosoma length-test just body length)/test just body length] * 100%

Body weight increase rate (%)=[(test opisthosoma weight-test just body weight)/test just body weight] * 100%

Survival rate (%)=(end number-initial mantissa) * 100%

2, the detection of hemocyte phagocytic activity

With the Candida albicans inclined-plane of the flushed activation of physiological saline solution, through coming, the bacterium making 1.4*107/ml alive is hanged Liquid.Carry out inoculation test by feeding above-mentioned 7 groups of Penaeus merguiensis de man.s of 45 days, often organize and take 20 tail prawns at random, in prawn the second uromere Place carries out the bacteria suspension of intramuscular inoculation 0.1ml.Use 1ml blood collection needle after 1h and make blood smear glass according to a conventional method Sheet, dyeing, detects 100 hemocytees, calculates phagocytic rate and phagocytic index.Computing formula is as follows:

Percentage phagocytosis (PP)=100 hemocyte is joined the cell number/100*100% of phagocytosis

Phagocytic index (PI)=100 participates in intracellular total number of fungi/100 of phagocytosis

Experimental result is as follows:

The growth of 23 7 groups of Penaeus merguiensis de man.s of table, weightening finish and survival rate situation

Note: same column letter is identical to be indicated without significant difference (P > 0.05)；Letter is different, then there were significant differences (P < 0.05).

Embodiment of the present invention gained additive is compared with normal diet as can be seen from the above data, and gaining effect is obvious, Rapidly, survival rate is high in growth.

The detection of hemocyte phagocytic activity before and after table 24 test

Group	Phagocytic index	Phagocytic rate
			Additive 1 group	4.322±0.365^b	43.21±1.35^b
Additive 2 groups	4.246±0.476^b	42.79±1.24^b
			Additive 3 groups	4.187±0.311^b	41.26±0.79^b
Additive 4 groups	3.846±0.206^b	38.53±0.32^b
			Additive 5 groups	3.962±0.415^b	39.79±0.63^b
Additive 6 groups	4.013±0.621^b	40.31±0.37^b
			Matched group	1.537±0.687^a	16.73±1.64^a

Note: colleague's letter is identical to be indicated without significant difference (P > 0.05)；Letter is different, then there were significant differences (P < 0.05).

Embodiment of the present invention gained additive is compared with normal diet as can be seen from the above data, and immunity has obtained pole Big raising.

3, choose sick shrimp 140 tail suffering from Baculovirus Diseases of Penaeus monodon in this Penaeus merguiensis de man. plant, be randomly divided into 7 Group, often organizes 20 tails.7 groups respectively feed the embodiment of the present invention 1 additive 1 group, feed the embodiment of the present invention 2 additive 2 groups, raise Feed the embodiment of the present invention 3 additive 3 groups, feed the embodiment of the present invention 4 additive 4 groups, feed the embodiment of the present invention 5 additive 5 Group, feed the embodiment of the present invention 6 additive 6 groups and feed the contrast groups of common additives group.Additive quality accounts for feedstuff gross weight The 0.1% of amount.Being put in a suitable place to breed respectively in the net cage of 1.0m*1.0m*0.5m by 7 groups of Penaeus merguiensis de man.s, experimental period is 7 days.Daily management Every day increase the DO once.During test, feeding volume is thrown something and fed by the 7-9% of Penaeus merguiensis de man. body weight, throw raise 3 times/d of frequency (on Noon 7:00-8:00,15:00-16:00 in afternoon, 22:00-23:00 in evening), early, middle and late feeding volume is respectively 35%, 30% With 35%.

Symptom:

Pathogenic shrimp poor appetite, movable dull, the gill, appendage and body surface are often with substantial amounts of epizoite.Children after infected Body, in addition to part body colour is deepened, there is no special symptom, and it is the most normal that majority takes viruliferous shrimp activity.

Efficacy determination:

Recovery from illness: appetite and activity are normal, and the gill, appendage and body surface epizoite disappear；

Effective: appetite and movable improvement, the gill, appendage and body surface epizoite symptom alleviate；

Invalid: dead.

Experimental result:

After 140 tail disease shrimps are fed 4 days by table 25, experimental result:

Above experimental result, can be notable it can be seen that embodiment of the present invention gained additive is compared with common additives Improve immunity and the resistance against diseases of Penaeus merguiensis de man., the most aobvious to the Baculovirus Diseases of Penaeus monodon effect for the treatment of Penaeus merguiensis de man. Write, there is the instant effect of uniqueness, short treating period, the advantage that has no side effect.

To sum up experimental result, gained Penaeus merguiensis de man. additive of the present invention, relative to common additives, can significantly improve ink The immunity of lucky prawn, greatly improves the Penaeus merguiensis de man. speed of growth, can effectively treat Baculovirus Diseases of Penaeus monodon simultaneously, carry The high economic worth of Penaeus merguiensis de man..

The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all spirit in the present invention and Within principle, any modification, equivalent substitution and improvement etc. made, should be included within the scope of the present invention.

Claims

1. a Penaeus merguiensis de man. feed additive, it is characterised in that described additive includes following components: Giant salamander oil, Herba Portulacae, Fructus Chaenomelis, Herba Taraxaci, Fructus Luffae, Pericarpium Citri Reticulatae, Bulbus Lilii, Lentinus Edodes, Herba Menthae, Folium Mori, Fructus actinidiae chinensis, Herba Epilobii Pyrricholophi, Canis familiaris L. ant grass, Radix Glycyrrhizae, flexuose bittercress herb and seed, Mel Jujubae, Pollen Brassicae campestris and vinegar-egg juice.

2. Penaeus merguiensis de man. feed additive as claimed in claim 1, it is characterised in that the weight of each raw material in described additive Number is: Giant salamander oil 0.5-1.0 part, Herba Portulacae 4-15 part, Fructus Chaenomelis 10-19 part, Herba Taraxaci 12-22 part, Fructus Luffae 4-11 part, Pericarpium Citri Reticulatae 15-28 part, Bulbus Lilii 8-14 part, Lentinus Edodes 5-16 part, Herba Menthae 9-19 part, Fructus actinidiae chinensis 3-14 part, Herba Epilobii Pyrricholophi 0.4-1.0 part, Canis familiaris L. ant grass 0.2-0.9 part, Radix Glycyrrhizae 4-12 part, flexuose bittercress herb and seed 0.4-1.2 part, Mel Jujubae 17-31 part, Pollen Brassicae campestris 10-23 part and vinegar-egg juice 7- 14 parts.

3. Penaeus merguiensis de man. feed additive as claimed in claim 1 or 2, it is characterised in that each raw material in described additive Parts by weight are: Giant salamander oil 0.6-0.8 part, Herba Portulacae 8-11 part, Fructus Chaenomelis 13-17 part, Herba Taraxaci 15-20 part, Fructus Luffae 5-9 part, Pericarpium Citri Reticulatae 18-22 part, Bulbus Lilii 10-12 part, Lentinus Edodes 8-13 part, Herba Menthae 11-16 part, Fructus actinidiae chinensis 6-11 part, Herba Epilobii Pyrricholophi 0.4-0.9 part, Canis familiaris L. ant grass 0.2-0.7 part, Radix Glycyrrhizae 4-11 part, flexuose bittercress herb and seed 0.4-1.0 part, Mel Jujubae 20-27 part, Pollen Brassicae campestris 14-19 part and vinegar Egg liquid 9-11 part.

4. the Penaeus merguiensis de man. feed additive as described in claims 1 to 3 is arbitrary, it is characterised in that each former in described additive Material parts by weight be: Giant salamander oil 0.8 part, Herba Portulacae 8 parts, Fructus Chaenomelis 15 parts, Herba Taraxaci 20 parts, 5 parts of Fructus Luffae, Pericarpium Citri Reticulatae 22 parts, hundred Close 10 parts, 8 parts of Lentinus Edodes, Herba Menthae 16 parts, Fructus actinidiae chinensis 8 parts, Herba Epilobii Pyrricholophi 0.4 part, Canis familiaris L. ant grass 0.7 part, 9 parts of Radix Glycyrrhizae, flexuose bittercress herb and seed 0.8 Part, Mel Jujubae 27 parts, Pollen Brassicae campestris 14 parts and vinegar-egg juice 11 parts.

5. the Penaeus merguiensis de man. feed additive as described in Claims 1-4 is arbitrary, it is characterised in that each former in described additive Material parts by weight be: Giant salamander oil 0.7 part, Herba Portulacae 11 parts, Fructus Chaenomelis 13 parts, Herba Taraxaci 18 parts, 7 parts of Fructus Luffae, Pericarpium Citri Reticulatae 18 parts, hundred Close 12 parts, 10 parts of Lentinus Edodes, Herba Menthae 14 parts, Fructus actinidiae chinensis 11 parts, Herba Epilobii Pyrricholophi 0.9 part, Canis familiaris L. ant grass 0.4 part, 4 parts of Radix Glycyrrhizae, flexuose bittercress herb and seed 1.0 Part, Mel Jujubae 20 parts, Pollen Brassicae campestris 16 parts and vinegar-egg juice 10 parts.

6. the Penaeus merguiensis de man. feed additive as described in claim 1 to 5 is arbitrary, it is characterised in that each former in described additive Material parts by weight be: Giant salamander oil 0.6 part, Herba Portulacae 9 parts, Fructus Chaenomelis 17 parts, Herba Taraxaci 15 parts, 9 parts of Fructus Luffae, Pericarpium Citri Reticulatae 20 parts, hundred Close 11 parts, 13 parts of Lentinus Edodes, Herba Menthae 11 parts, Fructus actinidiae chinensis 6 parts, Herba Epilobii Pyrricholophi 0.6 part, Canis familiaris L. ant grass 0.2 part, 11 parts of Radix Glycyrrhizae, flexuose bittercress herb and seed 0.4 Part, Mel Jujubae 23 parts, Pollen Brassicae campestris 19 parts and vinegar-egg juice 9 parts.

7. the preparation method of the Penaeus merguiensis de man. feed additive as described in claim 1 to 6 is arbitrary, it is characterised in that institute State preparation method to comprise the following steps:

(1) fatty tissue in Andrias davidianus Blanchard body is taken, chopping, clean up, add the deionized water of adipose tissue mass 5-7 times, military After fire boils, after change slow fire and boil 5-6h, obtained oil filtrating, remove slag, centrifugal, take supernatant fluid, obtain Giant salamander oil；

(2) take egg several, add 6 ° of rice vinegars of egg quality 3 times, seal and soak after 1 week, unpacking is by Ovum Gallus domesticus album and egg Huang stirs evenly with vinegar, after continuing to seal 2 days, crosses leaching filtrate and obtains vinegar-egg juice；

(3) Fructus Chaenomelis and Fructus Luffae are put into pulverizer to pulverize, take the Fructus Chaenomelis after Giant salamander oil, vinegar-egg juice, pulverizing by described mass fraction And Fructus Luffae, place 15-18h after mix homogeneously and obtain mixture one；

(4) Herba Portulacae, Herba Taraxaci, Pericarpium Citri Reticulatae, Bulbus Lilii, Lentinus Edodes, Herba Menthae, Folium Mori, Fructus actinidiae chinensis, the heart are taken in described mass fraction ratio Radix Gentianae, Canis familiaris L. ant grass, Radix Glycyrrhizae, flexuose bittercress herb and seed mixing, adding relative to the alcohol volumetric concentration of mixture 10～12 times is 90%～95% Ethanol, is heated to boiling reflux 3～5 hours, filters, and collects filtrate, subsequently concentrating under reduced pressure under vacuum 0.05～0.08Mpa To the mastic that relative density when 40～50 DEG C is 1.00～1.04, it is spray-dried, the inlet temperature 160～175 of spray dryer DEG C, leaving air temp 80～85 DEG C, be ground into powder subsequently, make dried cream powder；

(5) mixture one that described mass parts Mel Jujubae, Pollen Brassicae campestris and step (2) obtain, the dried cream powder that step (4) obtains are taken Mix homogeneously obtains Penaeus merguiensis de man. feed additive.