CN107927449A - The preparation method of microbial fermentation prawn feed - Google Patents
The preparation method of microbial fermentation prawn feed Download PDFInfo
- Publication number
- CN107927449A CN107927449A CN201711263095.6A CN201711263095A CN107927449A CN 107927449 A CN107927449 A CN 107927449A CN 201711263095 A CN201711263095 A CN 201711263095A CN 107927449 A CN107927449 A CN 107927449A
- Authority
- CN
- China
- Prior art keywords
- parts
- weight
- temperature
- powder
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Marine Sciences & Fisheries (AREA)
- Botany (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Sustainable Development (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Fodder In General (AREA)
Abstract
The present invention provides a kind of preparation method of microbial fermentation prawn feed, comprise the following steps：S1, add water mill into slurries after mixing freshwater mussel, river snail, silkworm chrysalis, adds pepsin, papain enzymolysis solution, obtains the first filter residue and supernatant；S2, after mixing kuh-seng, Eupatorium adenophorum, garden columbine, oriental blueberry leaf, Herba Corydalis, be heated to reflux, separate to obtain the second filter residue and filtrate；S3, by Bifidobacterium, lactic acid bacteria, bacillus natto be inoculated in primary culture medium and cultivate；S4, ferment after mixing corn flour, the first filter residue, the second filter residue and filtrate bacillus subtilis, chromic acid bacterium, obtains the first fermentate；By the first fermentate and clam shell powder mixed fermentation, filtering, dry the second fermentate；S5, the second fermentate of mixing, multi-vitamins, powder, are granulated, to obtain the final product.The prawn feed of the present invention can increase the function of immune system of prawn containing profitable probliotics, additionally it is possible to treat enteric flora disturbance, improve intestinal environment, promote enteron aisle unobstructed.
Description
Technical field
The present invention relates to prawn feed technical field, and in particular to a kind of preparation method of microbial fermentation prawn feed.
Background technology
Prawn culturing has the advantages that the speed of growth is fast, adaptable, yield is high, it has also become China's shrimps are most important
One of breed variety.But during shrimp is supported in high density industrialization, pollution of the high protein material to water body, shrimp excreta in feed
Pollution of the ammonia, nitrogen, hydrogen sulfide of formation to water body often causes the large quantities of morbidities of prawn dead.Prevent for the disease in breeding process
Control, common technological treatment, mainly largely use the disinfectant such as antibiotic, quaternary ammonium salt, Operand, not only heavy damage
The ecological balance, while can be remained in shrimp body, animal body normal colony balance is destroyed, causes animal body immune function
Decline, and the increase of antibody-resistant bacterium in animal body can be caused, further increase the difficulty of disease control.It is clear and definite due to lacking
Usage criteria, for a long time, the abuse of antibiotic and chemicals, constitutes ecological environment and human health very big prestige
The side of body.Therefore, it is badly in need of the feed environmentally friendly, pollution-free, disease resistance is strong.
The content of the invention
It is an object of the invention to solve at least the above, and provide the advantages of at least will be described later.
It is an object of the present invention to provide a kind of preparation method of microbial fermentation prawn feed, its by freshwater mussel, river snail,
Silkworm chrysalis is digested into small molecule using enzyme is conducive to assimilating for prawn, while can increase the immune system of prawn containing profitable probliotics
Function, additionally it is possible to treat enteric flora disturbance, improve intestinal environment, promote enteron aisle unobstructed.
It is an object of the present invention to provide a kind of preparation method of microbial fermentation prawn feed, comprise the following steps：
Add after S1, the freshwater mussel, 10~20 parts of river snail, 20~25 parts of silkworm chrysalis co-grinding that are 20~30 parts by parts by weight
Enter water mill that parts by weight are 200~250 parts into slurries, then add the pepsin that parts by weight are 0.1~0.2 part is in temperature
3~4h is digested at 35~40 DEG C, the papain that then addition parts by weight are 0.1~0.2 part is at 50~53 DEG C in temperature
5~6h is digested, it is 5~8h of enzymolysis at 58~62 DEG C to add flavor protease that parts by weight are 0.5~1 part in temperature, centrifugation
Separation, obtain the first filter residue and supernatant, then by supernatant concentration, be spray-dried to obtain powder；
S2, the kuh-seng by parts by weight are 3~5 parts, 2~6 parts of Eupatorium adenophorum, 3~5 parts of garden columbine, 3~5 parts of south
Candle leaf, 4~7 parts of Herba Corydalis, 3~6 parts of mouse bring up grass, 2~4 parts of the root of bidentate achyranthes, 3~6 parts of Radix Rhapontici seu Radix Echinopsis, 4~7 parts of Radix Isatidis, 3
~5 parts of the bark of eucommia, 0.5~1 part of rheum officinale, 2~4 parts of Echinacea, 3~5 parts of Poria cocos, 1~3 part of radix glycyrrhizae co-grinding
Afterwards, the water that addition parts by weight are 250~300 parts is to be heated to reflux 15~20h at 80~90 DEG C in temperature, separates to obtain the second filter residue
And filtrate；
S3, the Bifidobacterium, 0.5~1 part of lactic acid bacteria, 0.05~0.1 part of natto bar that are 0.5~1 part by parts by weight
Bacterium, 0.1~0.2 part of bacillus subtilis, 0.5~0.8 part of chromic acid bacterium be inoculated in primary culture medium in temperature for 35~
2~3h is cultivated at 37 DEG C；Then Bifidobacterium, lactic acid bacteria, bacillus natto, bacillus subtilis, chromic acid bacterium are inoculated in again
In Secondary media 2~3h is cultivated at temperature is 35~37 DEG C；Wherein, primary culture medium is 80~90 parts by parts by weight
Water, 20~30 parts of brown sugar, 5~10 parts of honey, 3~5 parts of glucose mix；Secondary media weight of oil part is 80
~90 parts of water, 20~30 parts of dehydrated potato powder, 5~10 parts of peptone, 1~2 part of sodium chloride, 3~5 parts of glucose,
0.1~0.5 part of natrii tauroglycocholas mixes；
S4, the corn flour by parts by weight are 10~15 parts, 15~20 parts of fish meal, 15~20 parts of rapeseed dregs, 15~20
Part dregs of beans, the first filter residue in S1, cultivated in the second filter residue in S2 and filtrate, S3 after Bifidobacterium, lactic acid bacteria, natto
Bacillus, bacillus subtilis, the mixing of chromic acid bacterium are the 10~15d that ferments at 50~60 DEG C after temperature, obtain the first fermentate；Then
By the first fermentate and clam shell powder in mass ratio 3~5:After 1 mixing, then ferment 2~3d at temperature is 60~70 DEG C, mistake
Filter, dry the second fermentate；
S5, be 100 in mass ratio:0.1~0.2:10~15 the second fermentates of mixing, multi-vitamins, powder, stirring are equal
It is even, it is granulated, drying, to obtain the final product.
Preferably, the preparation method of the microbial fermentation prawn feed, kuh-seng, Eupatorium adenophorum, garden columbine, south candle
After leaf, Herba Corydalis, mouse bring up grass, the root of bidentate achyranthes, Radix Rhapontici seu Radix Echinopsis, Radix Isatidis, the bark of eucommia, rheum officinale, Echinacea, Poria cocos, radix glycyrrhizae co-grinding, weight is added
Measure part to be 50~60 parts, soak 1~2h in the water that temperature is 95~100 DEG C, 10~12h then kept at -20~-25 DEG C,
Most after keeping 20~30min at 25~30 DEG C, reenter in the water that parts by weight are 250~300 parts and flow back.
Preferably, the preparation method of the microbial fermentation prawn feed, the preparation method of clam shell powder are：Will weight
After measuring the clam shell crushing that part is 30~40 parts, the phosphorus that the mass concentration that parts by weight are 50~60 parts is 10~15% is subsequently placed in
2~3h is soaked at temperature is 30~50 DEG C in acid solution, is taken out, is cleaned；Then the clam shell after cleaning is placed in parts by weight
In the sodium carbonate liquor that mass concentration for 20~30 parts is 5~8% soak 20~30min, take out, after cleaning, drying with again
After mixing, calcination, obtains clam shell powder to the far-infared ceramic powder that amount part is 0.5~1 part.
Preferably, the preparation method of the microbial fermentation prawn feed, calcination include prior to temperature for 300~
1~2h is calcined at 350 DEG C；Then 550~600 DEG C are warming up to as 6~8 DEG C/min using heating rate and keep 1~1.5h, then with
8~10 DEG C/min rate of temperature fall is cooled to 350~400 DEG C and keeps 30~40min, then again using heating rate as 10~12
DEG C/min be warming up to 700~750 DEG C and keep 0.5~1h, be subsequently cooled to room temperature, obtain clam shell powder.
Preferably, the preparation method of the microbial fermentation prawn feed, it is the second hair to further include quality in S5
5~10% phagostimulant of ferment amount of substance and the second fermentate, multi-vitamins, powder, mix；The system of wherein described phagostimulant
It is standby to comprise the following steps：
Step 1: by parts by weight be 1~2 part illiciumverum, 2~3 parts of fennel, 1~2 part of onion, 2~3 parts of garlic,
1~2 part of hawthorn, 2~3 parts of peppermint are added after crushing in the water that parts by weight are 30~40 parts, stirring, and are added parts by weight and be
0.1~0.5 part of 1~2h of complex enzyme zymohydrolysis, enzyme deactivation, obtains slurry；Wherein, it is 1 that complex enzyme, which includes mass ratio,:2~3:1~2:
1~2:2~3 lywallzyme, pectase, cellulase, protease and lipase；
Step 2: it is 5~10 parts of maize flour, 5~10 parts of shrimp shell, 2~5 parts of Pizza grass mixed grinding by parts by weight
Cuttlefish powder that Cheng Fenhou and parts by weight are 10~15 parts, 8~10 parts of albumen powder, 0.1~0.5 part of carvacrol, 5~10 parts
Slurry mixing, be granulated, up to phagostimulant.
Preferably, the preparation method of the microbial fermentation prawn feed, during the 10~15d that ferments in S4, every 2
~3d is passed through air and stirs, and the flow for being passed through air is 0.5~1m3/min；Obtain after the first fermentate and put in S4
It is that 10~20min is irradiated under the infrared lamp of 500~1000W in power, then carries out second of fermentation.
The present invention includes at least following beneficial effect：
1st, the preparation method of microbial fermentation prawn feed of the invention, freshwater mussel, river snail, silkworm chrysalis are digested into small using enzyme
Molecule is conducive to assimilating for prawn；The anti-nutritional factors in dregs of beans, silkworm chrysalis can be removed by fermenting twice, be conducive to seek
Support digesting and assimilating for material；Contain Bifidobacterium, lactic acid bacteria, bacillus natto, withered in the primary culture medium used in fermentation process
Careless bacillus, chromic acid bacterium, these probiotics can increase the function of immune system of prawn, additionally it is possible to enteric flora disturbance is treated,
Improve intestinal environment, promote enteron aisle unobstructed.
2nd, the preparation method of microbial fermentation prawn feed of the invention, by kuh-seng, Eupatorium adenophorum, garden columbine, radix glycyrrhizae etc.
Immersion, 10~12h of holding at -20~-25 DEG C in the water that raw material temperature is 95~100 DEG C, after raw material contacts hot water, carefully
Cell wall can expand, and have an effect that part cell reaches broken wall again, and the liquid water of the more same quality of volume after water freezing
Volume increase, moisture build-up ice in the short time trichite life volumetric expansion while, also quickly can fully burst plant medicinal material
Cell membrane, reach more effective shell-broken effect, so as to fully discharge the active ingredient of each raw material, substantially increase drug effect.This
A little herbal raw material components cooperate, cooperate with the disease resistance that can increase prawn.
3rd, the preparation method of microbial fermentation prawn feed of the invention, further includes clam shell, after clam shell is crushed simultaneously
First soaked with phosphoric acid solution and then with after soaking in sodium carbonate solution, and poroid distribution can be obtained after repeatedly segmentation calcination
Uniformly, fine and close porous material so that Nutrient distribution in feed is slowly discharged in hole, and extend feed has
The effect phase, while cavernous clam shell powder can be with the heavy metal in adsorbed water body, plankton, existing in a variety of manners
Inorganic and organic nitrogen, plays the effect of water purification；Far-infared ceramic powder is added in feed at the same time, the far-infared ceramic powder of addition exists
Promote porous formation in clam shell calcination, and make hole fine and close, while far-infared ceramic powder can kill bacterium in water, accelerate water
Molecular motion increases oxygen in water, improves water quality.
4th, the preparation method of microbial fermentation prawn feed of the invention, also contains phagostimulant, and the garlic in phagostimulant contains
There is allicin not only with good attractant effect also with bactericidal effect；There is special fragrance can increase for illiciumverum, hawthorn, peppermint
The appetite of prawn；Containing substantial amounts of amino acid and nucleotide is largely contained in shrimp shell, cuttlefish powder, albumen powder, can stimulate significantly
The smell of prawn, the sense of taste make it gather around feed, accelerate to ingest.
Further advantage, target and the feature of the present invention embodies part by following explanation, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Embodiment
With reference to embodiment, the present invention is described in further detail, to make those skilled in the art with reference to specification
Word can be implemented according to this.
It should be noted that experimental method described in following embodiments, is conventional method unless otherwise specified, institute
Reagent and material are stated, unless otherwise specified, is commercially obtained.
Embodiment 1
A kind of preparation method of microbial fermentation prawn feed, comprises the following steps：
Parts by weight are added after S1, the freshwater mussel for being 20 parts by parts by weight, 10 parts of river snail, 20 parts of silkworm chrysalis co-grinding is
For 200 parts of water mill into slurries, the pepsin that then addition parts by weight are 0.1 part is that 3h, Ran Houjia are digested at 35 DEG C in temperature
It is to digest 5h at 50 DEG C to enter papain that parts by weight are 0.1 part in temperature, adds the flavor albumen that parts by weight are 0.5 part
Enzyme is to digest 5h at 58 DEG C in temperature, centrifuges, obtains the first filter residue and supernatant, then by supernatant concentration, spray drying
Obtain powder；
S2, the kuh-seng by parts by weight are 3 parts, 2 parts of Eupatorium adenophorum, 3 parts of garden columbine, 3 parts of oriental blueberry leaf, 4 parts short
Corydalis, 3 parts of mouse bring up grass, 2 parts of the root of bidentate achyranthes, 3 parts of Radix Rhapontici seu Radix Echinopsis, 4 parts of Radix Isatidis, 3 parts of the bark of eucommia, 0.5 part of rheum officinale, 2 parts
After Echinacea, 3 parts of Poria cocos, 1 part of radix glycyrrhizae co-grinding, it in temperature is to be heated at 80 DEG C to add the water that parts by weight are 250 parts
Flow back 15h, separates to obtain the second filter residue and filtrate；
S3, the Bifidobacterium by parts by weight are 0.5 part, 0.5 part of lactic acid bacteria, 0.05 part of bacillus natto, 0.1 part withered
It is to cultivate 2h at 35 DEG C that careless bacillus, 0.5 part of chromic acid bacterium, which are inoculated in primary culture medium in temperature,；Then again by bifid bar
It is to be cultivated at 35 DEG C that bacterium, lactic acid bacteria, bacillus natto, bacillus subtilis, chromic acid bacterium, which are inoculated in Secondary media in temperature,
2h；Wherein, primary culture medium by parts by weight be 80 parts water, 20 parts of brown sugar, 5 parts of honey, 3 parts glucose mixing and
Into；Water that Secondary media weight of oil part is 80 parts, 20 parts of dehydrated potato powder, 5 parts of peptone, 1 part of sodium chloride, 3 parts
Glucose, 0.1 part of natrii tauroglycocholas mix；
S4, the corn flour by parts by weight are 10 parts, 15 parts of fish meal, 15 parts of rapeseed dregs, 15 parts of dregs of beans, the in S1
Bifidobacterium, lactic acid bacteria after being cultivated in the second filter residue and filtrate, S3 in one filter residue, S2, bacillus natto, bacillus subtilis
Bacterium, the mixing of chromic acid bacterium are the 10d that ferments at 50 DEG C after temperature, obtain the first fermentate；Then by the first fermentate and clam shell powder
In mass ratio 3:It is then to ferment 2d at 60 DEG C in temperature after 1 mixing, filtering, dry the second fermentate；
S5, be 100 in mass ratio:0.1:10 the second fermentates of mixing, multi-vitamins, powder, stir evenly, and is granulated,
Drying, to obtain the final product.
The preparation method of the microbial fermentation prawn feed, kuh-seng, Eupatorium adenophorum, garden columbine, oriental blueberry leaf, short purple
After violet, mouse bring up grass, the root of bidentate achyranthes, Radix Rhapontici seu Radix Echinopsis, Radix Isatidis, the bark of eucommia, rheum officinale, Echinacea, Poria cocos, radix glycyrrhizae co-grinding, adding parts by weight is
50 parts, temperature be to soak 1h in 95 DEG C of water, 10h is then kept at -20 DEG C, most after keeping 20min at 25 DEG C, reenters weight
Amount part is to flow back in 250 parts of water.
The preparation method of the microbial fermentation prawn feed, the preparation method of clam shell powder are：It is 30 by parts by weight
Part clam shell crush after, be subsequently placed in the phosphoric acid solution that the mass concentration that parts by weight are 50 parts is 10~15% and be in temperature
2h is soaked at 30 DEG C, is taken out, is cleaned；Then it is 5% the clam shell after cleaning to be placed in the mass concentration that parts by weight are 20 parts
20min is soaked in sodium carbonate liquor, is taken out, is uniformly mixed after cleaning, drying with the far-infared ceramic powder that parts by weight are 0.5 part
Afterwards, it is calcined, obtains clam shell powder.
The preparation method of the microbial fermentation prawn feed, it is to be calcined 1h at 300 DEG C that calcination, which is included prior to temperature,；So
550 DEG C are warming up to as 6 DEG C/min using heating rate afterwards and keep 1h, then be cooled to 350 DEG C with 8 DEG C/min rate of temperature fall and keep
30min, is then warming up to 700 DEG C as 10 DEG C/min using heating rate again and keeps 0.5h, be subsequently cooled to room temperature, obtain clam shell
Powder.
The preparation method of the microbial fermentation prawn feed, it is the second fermentate quality to further include quality in S5
5% phagostimulant and the second fermentate, multi-vitamins, powder, mix；The preparation of wherein described phagostimulant includes following step
Suddenly：
Step 1: it is 1 part of illiciumverum, 2 parts of fennel, 1 part of onion, 2 parts of garlic, 1 part of hawthorn, 2 by parts by weight
The peppermint of part is added after crushing in the water that parts by weight are 30 parts, stirring, and adds parts by weight as 0.1 part of complex enzyme zymohydrolysis 1h,
Enzyme deactivation, obtains slurry；Wherein, it is 1 that complex enzyme, which includes mass ratio,:2:1:1:2 lywallzyme, pectase, cellulase, protease
And lipase；
Step 2: by parts by weight be 5 parts maize flour, 5 parts of shrimp shell, 2 parts of Pizza grass mixed grinding Cheng Fenhou with again
The cuttlefish powder, 8 parts of albumen powder, 0.1 part of carvacrol, the mixing of 5 parts of slurry that part is 10 parts are measured, is granulated, up to phagostimulant.
Preferably, the preparation method of the microbial fermentation prawn feed, ferments in S4 during 10d, leads to per 2d
Enter air and stir, the flow for being passed through air is 0.5m3/min；Obtained in S4 after the first fermentate and be placed on power be
10min is irradiated under the infrared lamp of 500W, then carries out second of fermentation.
Embodiment 2
The preparation method of microbial fermentation prawn feed, comprises the following steps：
Parts by weight are added after S1, the freshwater mussel for being 25 parts by parts by weight, 15 parts of river snail, 23 parts of silkworm chrysalis co-grinding is
For 230 parts of water mill into slurries, the pepsin that then addition parts by weight are 0.2 part is that 4h, Ran Houjia are digested at 38 DEG C in temperature
It is to digest 5h at 53 DEG C to enter papain that parts by weight are 0.1 part in temperature, adds the flavor albumen that parts by weight are 0.8 part
Enzyme is to digest 7h at 60 DEG C in temperature, centrifuges, obtains the first filter residue and supernatant, then by supernatant concentration, spray drying
Obtain powder；
S2, the kuh-seng by parts by weight are 4 parts, 4 parts of Eupatorium adenophorum, 4 parts of garden columbine, 4 parts of oriental blueberry leaf, 6 parts short
Corydalis, 5 parts of mouse bring up grass, 3 parts of the root of bidentate achyranthes, 4 parts of Radix Rhapontici seu Radix Echinopsis, 5 parts of Radix Isatidis, 4 parts of the bark of eucommia, 0.8 part of rheum officinale, 3 parts
After Echinacea, 4 parts of Poria cocos, 2 parts of radix glycyrrhizae co-grinding, it in temperature is to be heated at 85 DEG C to add the water that parts by weight are 280 parts
Flow back 18h, separates to obtain the second filter residue and filtrate；
S3, the Bifidobacterium by parts by weight are 0.8 part, 0.8 part of lactic acid bacteria, 0.08 part of bacillus natto, 0.2 part withered
It is to cultivate 3h at 36 DEG C that careless bacillus, 0.6 part of chromic acid bacterium, which are inoculated in primary culture medium in temperature,；Then again by bifid bar
It is to be cultivated at 36 DEG C that bacterium, lactic acid bacteria, bacillus natto, bacillus subtilis, chromic acid bacterium, which are inoculated in Secondary media in temperature,
3h；Wherein, primary culture medium by parts by weight be 85 parts water, 25 parts of brown sugar, 8 parts of honey, 4 parts glucose mixing and
Into；Water that Secondary media weight of oil part is 85 parts, 25 parts of dehydrated potato powder, 8 parts of peptone, 2 parts of sodium chloride, 4 parts
Glucose, 0.3 part of natrii tauroglycocholas mix；
S4, the corn flour by parts by weight are 13 parts, 18 parts of fish meal, 18 parts of rapeseed dregs, 18 parts of dregs of beans, the in S1
Bifidobacterium, lactic acid bacteria after being cultivated in the second filter residue and filtrate, S3 in one filter residue, S2, bacillus natto, bacillus subtilis
Bacterium, the mixing of chromic acid bacterium are the 13d that ferments at 55 DEG C after temperature, obtain the first fermentate；Then by the first fermentate and clam shell powder
In mass ratio 4:It is then to ferment 3d at 65 DEG C in temperature after 1 mixing, filtering, dry the second fermentate；
S5, be 100 in mass ratio:0.2:12 the second fermentates of mixing, multi-vitamins, powder, stir evenly, and is granulated,
Drying, to obtain the final product.
The preparation method of the microbial fermentation prawn feed, kuh-seng, Eupatorium adenophorum, garden columbine, oriental blueberry leaf, short purple
After violet, mouse bring up grass, the root of bidentate achyranthes, Radix Rhapontici seu Radix Echinopsis, Radix Isatidis, the bark of eucommia, rheum officinale, Echinacea, Poria cocos, radix glycyrrhizae co-grinding, adding parts by weight is
55 parts, temperature be to soak 2h in 98 DEG C of water, 11h is then kept at -22 DEG C, most after keeping 25min at 28 DEG C, reenters weight
Amount part is to flow back in 280 parts of water.
The preparation method of the microbial fermentation prawn feed, the preparation method of clam shell powder are：It is 35 by parts by weight
After the clam shell of part crushes, it is 40 DEG C to be subsequently placed in the phosphoric acid solution that the mass concentration that parts by weight are 55 parts is 13% in temperature
Lower immersion 3h, takes out, and cleans；Then the clam shell after cleaning is placed in the carbonic acid that the mass concentration that parts by weight are 25 parts is 6%
25min is soaked in sodium solution, take out, after cleaning, drying with parts by weight be 0.8 part far-infared ceramic powder after mixing, forging
Burn, obtain clam shell powder.
The preparation method of the microbial fermentation prawn feed, it is to be calcined 2h at 330 DEG C that calcination, which is included prior to temperature,；So
580 DEG C are warming up to as 7 DEG C/min using heating rate afterwards and keep 1.5h, then be cooled to 380 DEG C with 9 DEG C/min rate of temperature fall and protect
35min is held, then 730 DEG C is warming up to as 11 DEG C/min using heating rate again and keeps 0.8h, be subsequently cooled to room temperature, obtain clam
Shell powder.
The preparation method of the microbial fermentation prawn feed, it is the second fermentate quality to further include quality in S5
8% phagostimulant and the second fermentate, multi-vitamins, powder, mix；The preparation of wherein described phagostimulant includes following step
Suddenly：
Step 1: by parts by weight be 1.5 parts illiciumverum, 2 parts of fennel, 1 part of onion, 2 parts of garlic, 1 part of hawthorn,
3 parts of peppermint is added after crushing in the water that parts by weight are 35 parts, stirring, and adds the complex enzyme zymohydrolysis 2h that parts by weight are 0.3 part,
Enzyme deactivation, obtains slurry；Wherein, it is 1 that complex enzyme, which includes mass ratio,:3:2:1:2 lywallzyme, pectase, cellulase, protease
And lipase；
Step 2: by parts by weight be 8 parts maize flour, 8 parts of shrimp shell, 3 parts of Pizza grass mixed grinding Cheng Fenhou with again
The cuttlefish powder, 9 parts of albumen powder, 0.3 part of carvacrol, the mixing of 8 parts of slurry that part is 13 parts are measured, is granulated, up to phagostimulant.
The preparation method of the microbial fermentation prawn feed, ferments in S4 during 13d, is passed through air per 3d and stirs
Mix, the flow for being passed through air is 0.8m3/min；Obtained in S4 after the first fermentate and be placed on the infrared lamp that power is 800W
Lower irradiation 15min, then carry out second of fermentation.
Embodiment 3
A kind of preparation method of microbial fermentation prawn feed, comprises the following steps：
Parts by weight are added after S1, the freshwater mussel for being 30 parts by parts by weight, 20 parts of river snail, 25 parts of silkworm chrysalis co-grinding is
For 250 parts of water mill into slurries, the pepsin that then addition parts by weight are 0.2 part is that 4h, Ran Houjia are digested at 40 DEG C in temperature
It is to digest 6h at 53 DEG C to enter papain that parts by weight are 0.2 part in temperature, adds the flavor protease that parts by weight are 1 part
In temperature be 62 DEG C at digest 8h, centrifuge, obtain the first filter residue and supernatant, then by supernatant concentration, be spray-dried
Powder；
S2, the kuh-seng by parts by weight are 5 parts, 6 parts of Eupatorium adenophorum, 5 parts of garden columbine, 5 parts of oriental blueberry leaf, 7 parts short
Corydalis, 6 parts of mouse bring up grass, 4 parts of the root of bidentate achyranthes, 6 parts of Radix Rhapontici seu Radix Echinopsis, 7 parts of Radix Isatidis, 5 parts of the bark of eucommia, 1 part of rheum officinale, 4 parts of purple
Bore chrysanthemum, 5 parts of Poria cocos, after 3 parts of radix glycyrrhizae co-grinding, it is to be heated back at 90 DEG C to add water that parts by weight are 300 parts in temperature
20h is flowed, separates to obtain the second filter residue and filtrate；
S3, the Bifidobacterium by parts by weight are 1 part, 1 part of lactic acid bacteria, 0.1 part of bacillus natto, 0.2 part of withered grass bud
It is to cultivate 3h at 37 DEG C that spore bacillus, 0.8 part of chromic acid bacterium, which are inoculated in primary culture medium in temperature,；Then again by Bifidobacterium,
It is to cultivate 3h at 37 DEG C that lactic acid bacteria, bacillus natto, bacillus subtilis, chromic acid bacterium, which are inoculated in Secondary media in temperature,；
Wherein, primary culture medium is mixed by parts by weight for 90 parts of water, 30 parts of brown sugar, 10 parts of honey, 5 parts of glucose；
Secondary media weight of oil part is 90 parts of water, 30 parts of dehydrated potato powder, 10 parts of peptone, 2 parts of sodium chloride, 5 parts of Portugal
Grape sugar, 0.5 part of natrii tauroglycocholas mix；
S4, the corn flour by parts by weight are 15 parts, 20 parts of fish meal, 20 parts of rapeseed dregs, 20 parts of dregs of beans, the in S1
Bifidobacterium, lactic acid bacteria after being cultivated in the second filter residue and filtrate, S3 in one filter residue, S2, bacillus natto, bacillus subtilis
Bacterium, the mixing of chromic acid bacterium are the 15d that ferments at 60 DEG C after temperature, obtain the first fermentate；Then by the first fermentate and clam shell powder
In mass ratio 5:It is then to ferment 3d at 70 DEG C in temperature after 1 mixing, filtering, dry the second fermentate；
S5, be 100 in mass ratio:0.2:15 the second fermentates of mixing, multi-vitamins, powder, stir evenly, and is granulated,
Drying, to obtain the final product.
Preferably, the preparation method of the microbial fermentation prawn feed, kuh-seng, Eupatorium adenophorum, garden columbine, south candle
After leaf, Herba Corydalis, mouse bring up grass, the root of bidentate achyranthes, Radix Rhapontici seu Radix Echinopsis, Radix Isatidis, the bark of eucommia, rheum officinale, Echinacea, Poria cocos, radix glycyrrhizae co-grinding, weight is added
Amount part is 60 parts, soaks 2h in the water that temperature is 100 DEG C, and 12h is then kept at -25 DEG C, is most kept after at 30 DEG C
30min, reenters in the water that parts by weight are 300 parts and flows back.
The preparation method of the microbial fermentation prawn feed, the preparation method of clam shell powder are：It is 40 by parts by weight
After the clam shell of part crushes, it is 50 DEG C to be subsequently placed in the phosphoric acid solution that the mass concentration that parts by weight are 60 parts is 15% in temperature
Lower immersion 3h, takes out, and cleans；Then the clam shell after cleaning is placed in the carbonic acid that the mass concentration that parts by weight are 30 parts is 8%
30min is soaked in sodium solution, take out, after cleaning, drying with parts by weight be 1 part far-infared ceramic powder after mixing, calcination,
Obtain clam shell powder.
The preparation method of the microbial fermentation prawn feed, calcination include prior to temperature be 350 DEG C at calcination 1~
2h；Then 600 DEG C are warming up to as 8 DEG C/min using heating rate and keep 1.5h, then 400 are cooled to 10 DEG C/min rate of temperature fall
DEG C and keep 40min, be then warming up to 750 DEG C as 12 DEG C/min using heating rate again and keep 1h, be subsequently cooled to room temperature, obtain
Clam shell powder.
The preparation method of the microbial fermentation prawn feed, it is the second fermentate quality to further include quality in S5
10% phagostimulant and the second fermentate, multi-vitamins, powder, mix；The preparation of wherein described phagostimulant includes following step
Suddenly：
Step 1: it is 2 parts of illiciumverum, 3 parts of fennel, 2 parts of onion, 3 parts of garlic, 2 parts of hawthorn, 3 by parts by weight
The peppermint of part is added after crushing in the water that parts by weight are 40 parts, stirring, and adds parts by weight as 0.5 part of complex enzyme zymohydrolysis 1h,
Enzyme deactivation, obtains slurry；Wherein, it is 1 that complex enzyme, which includes mass ratio,:3:2:2:3 lywallzyme, pectase, cellulase, protease
And lipase；
Step 2: by parts by weight be 10 parts maize flour, 10 parts of shrimp shell, 5 parts of Pizza grass mixed grinding Cheng Fenhou with
Parts by weight are 15 parts of cuttlefish powder, 10 parts of albumen powder, 0.5 part of carvacrol, 10 parts of slurry mix, and are granulated, lure to obtain the final product
Eat agent.
The preparation method of the microbial fermentation prawn feed, ferments in S4 during 15d, is passed through air per 3d and stirs
Mix, the flow for being passed through air is 1m3/min；Obtained in S4 after the first fermentate and be placed on the infrared lamp that power is 1000W
Lower irradiation 20min, then carry out second of fermentation.
Comparative example 1
With embodiment 1, difference is being added without clam shell powder in S4.
By 400 healthy prawns (average weight is 0.28~0.35g, body grows 2.55~2.68cm), 4 are randomly divided into
Group, is respectively adopted the prawn of phase between the forage feed shelling of embodiment 1~3, and control group uses the limited public affairs of Huai'an Condar feed
The forage feed of production is taken charge of, the results are shown in Table 1 for the prawn weight of measure each group, body length, survival rate and efficiency of feed utilization respectively：
1 different feeds of table influence the upgrowth situation of prawn
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Control group | |
| Rate of body weight gain (%) | 2186.36 | 2231.63 | 2256.39 | 1863.23 |
| Growth rate (%) | 208.32 | 211.36 | 218.32 | 178.36 |
| Survival rate (%) | 93 | 92 | 91 | 78 |
| Feed coefficient | 0.91 | 0.89 | 0.86 | 1.28 |
Each growth indexes of prawn for employing the nursing of the prawn feed of the present invention as can be seen from Table 1 are shown than control group
Writing improves；The result shows that the Feed Energy rapidly promotes prawn growth, efficiency of feed utilization can be significantly improved.
By the prawn of 400 5 healthy~10g, be randomly divided into 2 groups, using the feed of embodiment 1 as experimental group, adopt
The forage feed produced with Kangda Forage Co., Ltd., Huaian as a control group, after feeding 60d, tests every group of average weight gain
Rate and survival number；Then 6 are randomly choosed in each group, are placed in ice pan and are dissected rapidly, take anterior intestine with 0.86% physiology salt
Water is rinsed well, is fixed in BouinShi liquid after 24h, carries out paraffin section, measurement and calculating enteron aisle average fluff height, wrinkle
Folds in a garment width, pleat height；Take 6 prawns at random respectively in each group, take anterior intestine to smash rear water-bath homogenate to pieces, centrifuging and taking supernatant is surveyed
Equal enteron aisle trypsase, lipase and amylase are allocated, the results are shown in Table 2.
As can be seen from Table 2 after the feed of the present invention is fed, the height of naps of gut of shrimp, pleat width, pleat
Control group is highly above, and the trypsase of enteron aisle, lipase and amylase activity are also significantly greater than control group.Illustrate this
The feed of invention can promote intestinal epithelial cell to grow, and strengthen the abilities of digestive and absorption at enteron aisle dominant digestion position, promote intestines
Road digests enzyme secretion, improves gut of shrimp function.
2 different feeds of table are to gut of shrimp morphology influence
| Experimental group | Control group | |
| Height of naps (um) | 1.18 | 0.89 |
| Pleat height (um) | 38.52 | 31.13 |
| Pleat width (um) | 27.26 | 23.18 |
| Trypsase (U/mgprot) | 1412.52 | 1038.36 |
| Lipase (U/mgprot) | 22.12 | 18.53 |
| Amylase (U/mgprot) | 179.45 | 136.36 |
Prawn feed prepared by prawn feed and comparative example 1 prepared by embodiment 1, carries out cultivation ratio under the same conditions
Compared with, and the index for cultivating raw water is：DO is 4.28mg/L, and COD 16.73mg/L, TN 1.38mg/L, TP are
0.083mg/L, measures front and rear change of water quality in breeding process, the results are shown in Table 3；
Table 3 cultivates front and rear change of water quality
The feed of embodiment 1 is in cultured prawn breeding process as can be seen from Table 3, DO increases, COD, TN, TP under
Drop, water quality reach cultivation water quality standard.The feed cultured prawn of comparative example 1, although indices are also superior to raw water, embodiment 1
Feed in contrast, the incrementss in cultivation early period and later stage DO are all higher than the incrementss of control group, the decline of COD, TN, TP
Amount is all higher than the slippage of control group, therefore the feed of the present invention has good purifying water effect.
Although embodiment of the present invention is disclosed as above, it is not restricted in specification and embodiment listed
With it can be applied to various suitable the field of the invention completely, can be easily for those skilled in the art
Realize other modification, therefore under the universal limited without departing substantially from claim and equivalency range, it is of the invention and unlimited
In specific details.
Claims (6)
1. a kind of preparation method of microbial fermentation prawn feed, it is characterised in that comprise the following steps：
Weight is added after S1, the freshwater mussel, 10~20 parts of river snail, 20~25 parts of silkworm chrysalis co-grinding that are 20~30 parts by parts by weight
Water mill that part is 200~250 parts is measured into slurries, then add pepsin that parts by weight are 0.1~0.2 part in temperature be 35~
3~4h is digested at 40 DEG C, the papain that then addition parts by weight are 0.1~0.2 part is to digest 5 at 50~53 DEG C in temperature
~6h, it is 5~8h of enzymolysis at 58~62 DEG C to add flavor protease that parts by weight are 0.5~1 part in temperature, is centrifuged,
The first filter residue and supernatant, then by supernatant concentration, be spray-dried to obtain powder；
S2, by parts by weight be 3~5 parts kuh-seng, 2~6 parts of Eupatorium adenophorum, 3~5 parts of garden columbine, 3~5 parts of oriental blueberry leaf,
4~7 parts of Herba Corydalis, 3~6 parts of mouse bring up grass, 2~4 parts the root of bidentate achyranthes, 3~6 parts of Radix Rhapontici seu Radix Echinopsis, 4~7 parts of Radix Isatidis, 3~5 parts
The bark of eucommia, 0.5~1 part of rheum officinale, 2~4 parts of Echinacea, 3~5 parts of Poria cocos, after 1~3 part of radix glycyrrhizae co-grinding, add
The water that parts by weight are 250~300 parts is to be heated to reflux 15~20h at 80~90 DEG C in temperature, separates to obtain the second filter residue and filtrate；
S3, by parts by weight be 0.5~1 part Bifidobacterium, 0.5~1 part of lactic acid bacteria, 0.05~0.1 part of bacillus natto,
It is 35~37 DEG C that 0.1~0.2 part of bacillus subtilis, 0.5~0.8 part of chromic acid bacterium, which are inoculated in primary culture medium in temperature,
2~3h of lower culture；Then Bifidobacterium, lactic acid bacteria, bacillus natto, bacillus subtilis, chromic acid bacterium are inoculated in secondary again
In culture medium 2~3h is cultivated at temperature is 35~37 DEG C；Wherein, primary culture medium by parts by weight be 80~90 parts water, 20
~30 parts of brown sugar, 5~10 parts of honey, 3~5 parts of glucose mix；Secondary media weight of oil part is 80~90
Part water, 20~30 parts of dehydrated potato powder, 5~10 parts of peptone, 1~2 part of sodium chloride, 3~5 parts of glucose, 0.1~
0.5 part of natrii tauroglycocholas mixes；
S4, the corn flour by parts by weight are 10~15 parts, 15~20 parts of fish meal, 15~20 parts of rapeseed dregs, 15~20 parts
Bifidobacterium, lactic acid bacteria after being cultivated in the second filter residue and filtrate, S3 in the first filter residue, S2 in dregs of beans, S1, natto bar
Bacterium, bacillus subtilis, the mixing of chromic acid bacterium are the 10~15d that ferments at 50~60 DEG C after temperature, obtain the first fermentate；Then will
First fermentate and clam shell powder in mass ratio 3~5:After 1 mixing, then ferment 2~3d at temperature is 60~70 DEG C, mistake
Filter, dry the second fermentate；
S5, be 100 in mass ratio:0.1~0.2:10~15 the second fermentates of mixing, multi-vitamins, powder, stir evenly,
It is granulated, drying, to obtain the final product.
2. the preparation method of microbial fermentation prawn feed as claimed in claim 1, it is characterised in that kuh-seng, Eupatorium adenophorum,
Garden columbine, oriental blueberry leaf, Herba Corydalis, mouse bring up grass, the root of bidentate achyranthes, Radix Rhapontici seu Radix Echinopsis, Radix Isatidis, the bark of eucommia, rheum officinale, Echinacea, Poria cocos, radix glycyrrhizae mixed powder
After broken, add parts by weight and be 50~60 parts, 1~2h is soaked in the water that temperature is 95~100 DEG C, then protected at -20~-25 DEG C
10~12h is held, most after keeping 20~30min at 25~30 DEG C, reenters in the water that parts by weight are 250~300 parts and flows back.
3. the preparation method of microbial fermentation prawn feed as claimed in claim 1, it is characterised in that the preparation of clam shell powder
Method is：After the clam shell that parts by weight are 30~40 parts is crushed, being subsequently placed in the mass concentration that parts by weight are 50~60 parts is
2~3h is soaked at temperature is 30~50 DEG C in 10~15% phosphoric acid solution, is taken out, is cleaned；Then by the clam after cleaning
Shell is placed in 20~30min of immersion in the sodium carbonate liquor that the mass concentration that parts by weight are 20~30 parts is 5~8%, takes out, washes
Only, after dry with far-infared ceramic powder that parts by weight are 0.5~1 part after mixing, calcination, obtains clam shell powder.
4. the preparation method of microbial fermentation prawn feed as claimed in claim 3, it is characterised in that calcination is included prior to temperature
Spend to be calcined 1~2h at 300~350 DEG C；Then using heating rate as 6~8 DEG C/min be warming up to 550~600 DEG C and keep 1~
1.5h, then be cooled to 350~400 DEG C with 8~10 DEG C/min rate of temperature fall and keep 30~40min, then again with heating rate
700~750 DEG C are warming up to for 10~12 DEG C/min and keeps 0.5~1h, are subsequently cooled to room temperature, are obtained clam shell powder.
5. the preparation method of microbial fermentation prawn feed as claimed in claim 4, it is characterised in that further included matter in S5
Measure 5~10% phagostimulant for the second fermentate quality and the second fermentate, multi-vitamins, powder, mix；It is wherein described
The preparation of phagostimulant comprises the following steps：
Step 1: it is 1~2 part of illiciumverum, 2~3 parts of fennel, 1~2 part of onion, 2~3 parts of garlic, 1~2 by parts by weight
The hawthorn of part, 2~3 parts of peppermint are added after crushing in the water that parts by weight are 30~40 parts, stirring, and it is 0.1 to add parts by weight
~0.5 part of 1~2h of complex enzyme zymohydrolysis, enzyme deactivation, obtains slurry；Wherein, it is 1 that complex enzyme, which includes mass ratio,:2~3:1~2:1~
2:2~3 lywallzyme, pectase, cellulase, protease and lipase；
Step 2: by maize flour that parts by weight are 5~10 parts, 5~10 parts of shrimp shell, 2~5 parts of Pizza grass mixed grinding into powder
It is afterwards 10~15 parts of cuttlefish powder, 8~10 parts of albumen powder, 0.1~0.5 part of carvacrol, 5~10 parts of slurry with parts by weight
Material mixing, is granulated, up to phagostimulant.
6. the preparation method of microbial fermentation prawn feed as claimed in claim 1, it is characterised in that in S4 ferment 10~
During 15d, every 2~3d is passed through air and stirs, and the flow for being passed through air is 0.5~1m3/min；The first fermentation is obtained in S4
After thing and 10~20min of irradiation under the infrared lamp that power is 500~1000W is placed on, then carries out second of fermentation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711263095.6A CN107927449A (en) | 2017-12-04 | 2017-12-04 | The preparation method of microbial fermentation prawn feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711263095.6A CN107927449A (en) | 2017-12-04 | 2017-12-04 | The preparation method of microbial fermentation prawn feed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107927449A true CN107927449A (en) | 2018-04-20 |
Family
ID=61944702
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711263095.6A Pending CN107927449A (en) | 2017-12-04 | 2017-12-04 | The preparation method of microbial fermentation prawn feed |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107927449A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108719690A (en) * | 2018-06-11 | 2018-11-02 | 安徽宜丰生态农业科技有限公司 | A kind of prawn feed and preparation method thereof |
| CN110663599A (en) * | 2018-07-02 | 2020-01-10 | 成都硕谷农业科技有限公司 | Method for breeding shrimps in fattening rice field |
| CN110710593A (en) * | 2018-07-13 | 2020-01-21 | 成都硕谷农业科技有限公司 | Lobster feed for shrimp culture in rice field |
| CN110710596A (en) * | 2018-07-13 | 2020-01-21 | 成都硕谷农业科技有限公司 | Feed for breeding lobsters in rice field |
| WO2020018013A1 (en) * | 2018-07-16 | 2020-01-23 | Shizenature Pte. Ltd. | Shrimp feed additive and method for producing the same |
| RU2826314C1 (en) * | 2024-07-16 | 2024-09-09 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Оренбургский государственный университет" | Method of increasing fish productivity |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101530232A (en) * | 2009-04-20 | 2009-09-16 | 广东环西保健科技股份有限公司 | Method for extracting compound amino acid from silkworm pupae by enzymatic method |
| CN102845648A (en) * | 2012-09-28 | 2013-01-02 | 河北科技师范学院 | Chinese herbal medicine feed additive for enhancing growth of prawns |
| CN105124166A (en) * | 2015-09-11 | 2015-12-09 | 江苏振宇环保科技有限公司 | Microbiological feed for increasing efficiency of feed utilization |
| CN105166553A (en) * | 2015-09-28 | 2015-12-23 | 佛山市高明区杨和镇金峰水产养殖场 | Shrimp feed and producing method thereof |
| CN106689742A (en) * | 2016-12-16 | 2017-05-24 | 广西康佳龙农牧集团有限公司 | Production process of fermented soybean meal |
| CN107212191A (en) * | 2017-06-15 | 2017-09-29 | 周代伟 | The preparation method of penaeus vannamei boone feed rich in polypeptide |
| CN107279477A (en) * | 2016-03-31 | 2017-10-24 | 江苏银宝生物科技有限公司 | The formula and processing method of a kind of Penaeus Vannmei biological feedstuff of high usage |
| CN107319128A (en) * | 2017-08-04 | 2017-11-07 | 池州市兴牧饲料有限公司 | A kind of Chinese herbal feed additive |
-
2017
- 2017-12-04 CN CN201711263095.6A patent/CN107927449A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101530232A (en) * | 2009-04-20 | 2009-09-16 | 广东环西保健科技股份有限公司 | Method for extracting compound amino acid from silkworm pupae by enzymatic method |
| CN102845648A (en) * | 2012-09-28 | 2013-01-02 | 河北科技师范学院 | Chinese herbal medicine feed additive for enhancing growth of prawns |
| CN105124166A (en) * | 2015-09-11 | 2015-12-09 | 江苏振宇环保科技有限公司 | Microbiological feed for increasing efficiency of feed utilization |
| CN105166553A (en) * | 2015-09-28 | 2015-12-23 | 佛山市高明区杨和镇金峰水产养殖场 | Shrimp feed and producing method thereof |
| CN107279477A (en) * | 2016-03-31 | 2017-10-24 | 江苏银宝生物科技有限公司 | The formula and processing method of a kind of Penaeus Vannmei biological feedstuff of high usage |
| CN106689742A (en) * | 2016-12-16 | 2017-05-24 | 广西康佳龙农牧集团有限公司 | Production process of fermented soybean meal |
| CN107212191A (en) * | 2017-06-15 | 2017-09-29 | 周代伟 | The preparation method of penaeus vannamei boone feed rich in polypeptide |
| CN107319128A (en) * | 2017-08-04 | 2017-11-07 | 池州市兴牧饲料有限公司 | A kind of Chinese herbal feed additive |
Non-Patent Citations (1)
| Title |
|---|
| 冯万祥等: "《生化技术》", 30 November 1989, 湖南科学技术出版社 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108719690A (en) * | 2018-06-11 | 2018-11-02 | 安徽宜丰生态农业科技有限公司 | A kind of prawn feed and preparation method thereof |
| CN110663599A (en) * | 2018-07-02 | 2020-01-10 | 成都硕谷农业科技有限公司 | Method for breeding shrimps in fattening rice field |
| CN110710593A (en) * | 2018-07-13 | 2020-01-21 | 成都硕谷农业科技有限公司 | Lobster feed for shrimp culture in rice field |
| CN110710596A (en) * | 2018-07-13 | 2020-01-21 | 成都硕谷农业科技有限公司 | Feed for breeding lobsters in rice field |
| WO2020018013A1 (en) * | 2018-07-16 | 2020-01-23 | Shizenature Pte. Ltd. | Shrimp feed additive and method for producing the same |
| RU2826314C1 (en) * | 2024-07-16 | 2024-09-09 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Оренбургский государственный университет" | Method of increasing fish productivity |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107927449A (en) | The preparation method of microbial fermentation prawn feed | |
| CN101263862B (en) | Food and rink castoff feed processing method and culture medium and confecting method thereby | |
| CN105076875A (en) | Ecological pig feed | |
| CN103667220B (en) | Neutral protease-containing growing pig dedicated enzyme and preparation method thereof | |
| CN106260504A (en) | A kind of method utilizing beer yeast slurry to produce fermentable moist forage | |
| CN105029131A (en) | Ecological fish feed | |
| CN106954760A (en) | It is a kind of into Bao special compound feed small powder and preparation method thereof | |
| CN106417915A (en) | Bream parent fish feed additive | |
| CN110037166A (en) | A kind of preparation method of Procambius clarkii Chinese herbal medicine composite fermented feed | |
| CN107319196A (en) | A kind of microorganism formulation for improving fresh-water fishes function of intestinal canal and application thereof | |
| CN103829042A (en) | Production method of multi-vitamin active cassava protein feed | |
| CN105941824A (en) | Traditional Chinese medicine microbial probiotic original liquid, feed, fertilizer and pesticide, and preparation methods thereof | |
| CN102367418A (en) | Traditional Chinese medicine probiotics liquid medium used for pig feed, culturing method and feed additive | |
| CN108633626A (en) | The method for preparing White mushroom cultivation base as primary raw material using cattle pen bedding and padding | |
| CN108402338A (en) | A kind of grouper liver protection type fermentation of Chinese herbal medicine feed addictive and the preparation method and application thereof, epinephelus feed | |
| CN105104874A (en) | Ecological turtle feed | |
| CN107897574A (en) | Improve the bream feed of disease resistance | |
| CN107821873A (en) | The preparation method of the feed of gut of shrimp function can be improved | |
| CN106616001A (en) | Novel tilapia feed produced from cassava lees through microbial fermentation and preparation method of tilapia feed | |
| CN105029130A (en) | Ecological donkey feed | |
| CN114747694A (en) | Sturgeon growth-promoting and immunity-increasing type compound Chinese herbal medicine fermentation preparation and preparation method thereof | |
| CN104604503B (en) | A kind of Biological control anti-root of Rhodiola sachalinensis maize ear rot and the method for growth-promoting | |
| CN103740683B (en) | A kind of Wheat ration enzyme containing neutral protease and preparation method thereof | |
| CN106343268A (en) | Fish feed containing Chinese herbal medicine formula and preparation method of fish feed | |
| CN105053732A (en) | Ecological rabbit feed |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180420 |
|
| RJ01 | Rejection of invention patent application after publication |