CN105124166A - Microbiological feed for increasing efficiency of feed utilization - Google Patents
Microbiological feed for increasing efficiency of feed utilization Download PDFInfo
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- CN105124166A CN105124166A CN201510576168.1A CN201510576168A CN105124166A CN 105124166 A CN105124166 A CN 105124166A CN 201510576168 A CN201510576168 A CN 201510576168A CN 105124166 A CN105124166 A CN 105124166A
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Abstract
The invention discloses microbiological feed for increasing the efficiency of feed utilization and belongs to the technical field of preparing of the microbiological feed. The microbiological feed disclosed by the invention is prepared through the steps of preparing a primary culture medium from potato extract flour, glucose and agar powder, preparing a secondary culture medium from the potato extract flour, peptone, glucose and sodium chloride, carrying out inoculated culturing, adding taurocholate sodium into the inoculated culture, carrying out static culturing, carrying out screening so as to obtain an aged bacterial solution of lactobacilli, saccharomycetes, photosynthetic bacteria and chromic acid bacteria, then, inoculating the aged bacterial solution to a high-temperature sterilized wheat-bran culture medium, and carrying out anaerobic fermentation and drying. The microbiological feed has the beneficial effects that the microbiological feed prepared by the method can be used for effectively increasing the utilization ratio of other roughage raw materials, the feed cost can be reduced to 5% of the original cost, the rapid transformation of nutritional ingredients of the other roughage raw materials is promoted, the digestion and absorption effects of the feed are enhanced, the consumption of the feed is decreased by 5-8%, and the efficiency of feed utilization reaches 95% or more and is increased by 8-10% compared with that before adding the microbiological feed.
Description
Technical field
The present invention relates to a kind of microbiological feed improving efficiency of feed utilization, belong to microbiological feed preparing technical field.
Background technology
Along with the continuous expansion of current feed industry market scale, and the speed with annual about 15% increases, people investigated a kind of microbiological feed that feedstuff nutrition transforms that promotes, it be with microorganism, complex enzyme for biological feed fermentation agent bacterial classification, feedstuff is converted into microbial bacteria body protein, bioactive micro peptide amino acid, microbial activity probio, complex enzyme formulation is integrated biological fermentation feed.This product not only can make up the amino acid easily lacked in conventional feed, and other roughage raw material nutrition can be made to transform rapidly, reaches enhancing and digests and absorbs effect.But the microbiological feed occurred in the market adds in other roughage raw materials, and nutritional labeling transformation time is long, digests and assimilates weak effect, and utilization rate is not high.Therefore, work out a kind of microbiological feed that can improve efficiency of feed utilization, both can reduce feeding cost, promote again the development of feed industry.
Summary of the invention
Technical problem to be solved by this invention: add in other roughage raw materials for current microbiological feed, nutritional labeling transformation time is long, cause digesting and assimilating weak effect, the drawback that feedstuff utilization rate is not high, provide a kind of microbiological feed improving efficiency of feed utilization, the preparation of this microbiological feed is simple, effectively can improve the utilization rate of other roughage raw materials, promote the rapid conversion of other roughage raw material Middle nutrition compositions, strengthen and digest and assimilate effect.
For solving the problems of the technologies described above, the present invention adopts technical scheme as described below to be: a kind of microbiological feed improving efficiency of feed utilization, and its concrete preparation process is:
(1) by mass percentage, choose the potato leaching powder solution of the 3g/L of 30 ~ 40%, the glucose solution of 20g/L of 30 ~ 45% and the agar powder solution of the 15g/L of 20 ~ 35% make primary culture medium, again by mass percentage, choose the potato leaching powder solution of the 5g/L of 25 ~ 30%, the peptone solution of the 10g/L of 20 ~ 25%, the glucose solution of 15g/L of 20 ~ 30% and the sodium chloride solution of the 5g/L of 20 ~ 30% make secondary medium;
(2) by 12 strain candidate lactic acid bacteria strains, 12 strain photosynthetic bacterias, the activated rear access primary culture medium of 12 strain chromic acid bacterium, its pH value and lactic bacteria strain number is measured respectively after Anaerobic culturel 24 ~ 36h, primary dcreening operation obtains producing acid and growing lactic acid bacteria strains faster, from photosynthetic bacteria and chromic acid bacteria strain, measure 4 the highest strain photosynthetic bacterias of bacterial strain and chromic acid bacteria strain, continued cultivation for subsequent use; Again saccharomycete is put Yeasts on starch culture-medium, after cultivating 18 ~ 24h, choose the highest yeast strain of 6 ~ 8 strain bacterial strains, continue to cultivate, for subsequent use;
(3) by the lactic acid bacteria of above-mentioned cultivation, saccharomycete, photosynthetic bacteria and chromic acid bacteria strain liquid by 10% inoculum concentration to be inoculated in pH be respectively on the secondary medium of 2 ~ 3, be in the secondary medium of the natrii tauroglycocholas of 0.4% by the inoculum concentration cut-in quality mark of 1% again after bacterium liquid being collected after quiescent culture 2h at 37 DEG C, can complete bacterial strain postsearch screening to its collection after quiescent culture 2h at 37 DEG C, for subsequent use;
(4) primary raw material is made by wheat bran, after wheat bran high-temperature sterilization, the ripe bacterium liquid of above-mentioned screening lactic acid bacteria for subsequent use, saccharomycete, photosynthetic bacteria and chromic acid bacterium is inoculated in the ratio of 3:3:3:1, total inoculum concentration is 10% of bran mass gross mass, to its anaerobic fermentation 2 ~ 3d at 25 ~ 30 DEG C, after fermentation completely at 20 ~ 25 DEG C low temperature drying 20 ~ 30h, a kind of microbiological feed improving efficiency of feed utilization can be obtained.
Application process of the present invention: obtained microbiological feed is added in other roughage raw materials, addition is 50 ~ 100g/kg, stir 10 ~ 15min, mix, throw something and feed, after 1 month, feed consumption decreases 5 ~ 8%, efficiency of feed utilization reaches more than 95%, before not adding, improve 8 ~ 10%.
The present invention is compared with additive method, and Advantageous Effects is:
(1) microbiological feed obtained by this method effectively can improve the utilization rate of other roughage raw materials, and feed cost can be made to be reduced to original 5%;
(2) promote the rapid conversion of other roughage material nutrient components, what strengthen feed digests and assimilates effect.
Detailed description of the invention
First by mass percentage, choose the potato leaching powder solution of the 3g/L of 30 ~ 40%, the glucose solution of 20g/L of 30 ~ 45% and the agar powder solution of the 15g/L of 20 ~ 35% make primary culture medium, again by mass percentage, choose the potato leaching powder solution of the 5g/L of 25 ~ 30%, the peptone solution of the 10g/L of 20 ~ 25%, the glucose solution of 15g/L of 20 ~ 30% and the sodium chloride solution of the 5g/L of 20 ~ 30% make secondary medium; By 12 strain candidate lactic acid bacteria strains, 12 strain photosynthetic bacterias, the activated rear access primary culture medium of 12 strain chromic acid bacterium, its pH value and lactic bacteria strain number is measured respectively after Anaerobic culturel 24 ~ 36h, primary dcreening operation obtains producing acid and growing lactic acid bacteria strains faster, from photosynthetic bacteria and chromic acid bacteria strain, measure 4 the highest strain photosynthetic bacterias of bacterial strain and chromic acid bacteria strain, continued cultivation for subsequent use; Again saccharomycete is put Yeasts on starch culture-medium, after cultivating 18 ~ 24h, choose the highest yeast strain of 6 ~ 8 strain bacterial strains, continue to cultivate, for subsequent use; Again by the lactic acid bacteria of above-mentioned cultivation, saccharomycete, photosynthetic bacteria and chromic acid bacteria strain liquid by 10% inoculum concentration to be inoculated in pH be respectively on the secondary medium of 2 ~ 3, be in the secondary medium of the natrii tauroglycocholas of 0.4% by the inoculum concentration cut-in quality mark of 1% again after bacterium liquid being collected after quiescent culture 2h at 37 DEG C, can complete bacterial strain postsearch screening to its collection after quiescent culture 2h at 37 DEG C, for subsequent use; Finally make primary raw material by wheat bran, after wheat bran high-temperature sterilization, the ripe bacterium liquid of above-mentioned screening lactic acid bacteria for subsequent use, saccharomycete, photosynthetic bacteria and chromic acid bacterium is inoculated in the ratio of 3:3:3:1, total inoculum concentration is 10% of bran mass gross mass, to its anaerobic fermentation 2 ~ 3d at 25 ~ 30 DEG C, after fermentation completely at 20 ~ 25 DEG C low temperature drying 20 ~ 30h, a kind of microbiological feed improving efficiency of feed utilization can be obtained.
Example 1
First by gross mass 100g, choose the potato leaching powder of the 3g/L of 30g, the agar powder of the glucose of the 20g/L of 35g and the 15g/L of 35g makes primary culture medium, again by gross mass 100g, choose the potato leaching powder of the 5g/L of 26g, the peptone of the 10g/L of 23g, the sodium chloride of the glucose of the 15g/L of 24g and the 5g/L of 27g makes secondary medium; By 12 strain candidate lactic acid bacteria strains, 12 strain photosynthetic bacterias, the activated rear access primary culture medium of 12 strain chromic acid bacterium, its pH value and lactic bacteria strain number is measured respectively after Anaerobic culturel 24h, primary dcreening operation obtains producing acid and growing lactic acid bacteria strains faster, from photosynthetic bacteria and chromic acid bacteria strain, measure 4 the highest strain photosynthetic bacterias of bacterial strain and chromic acid bacteria strain, continued cultivation for subsequent use; Again saccharomycete is put Yeasts on starch culture-medium, after cultivating 18h, choose the highest yeast strain of 6 strain bacterial strains, continue to cultivate, for subsequent use; Again by the lactic acid bacteria of above-mentioned cultivation, saccharomycete, photosynthetic bacteria and chromic acid bacteria strain liquid by 10% inoculum concentration to be inoculated in pH be respectively on the secondary medium of 2, be in the secondary medium of the natrii tauroglycocholas of 0.4% by the inoculum concentration cut-in quality mark of 1% again after bacterium liquid being collected after quiescent culture 2h at 37 DEG C, can complete bacterial strain postsearch screening to its collection after quiescent culture 2h at 37 DEG C, for subsequent use; Finally make primary raw material by wheat bran, after wheat bran high-temperature sterilization, the ripe bacterium liquid of above-mentioned screening lactic acid bacteria for subsequent use, saccharomycete, photosynthetic bacteria and chromic acid bacterium is inoculated in the ratio of 3:3:3:1, total inoculum concentration is 10% of bran mass gross mass, to its anaerobic fermentation 2d at 25 DEG C, after fermentation completely at 20 DEG C low temperature drying 20h, a kind of microbiological feed improving efficiency of feed utilization can be obtained.
Added to by obtained microbiological feed in other roughage raw materials, addition is 50g/kg, and stir 10min, mix, throw something and feed, after 1 month, feed consumption decreases 5%, and efficiency of feed utilization reaches 95.5%, before not adding, improve 8%.
Example 2
First by gross mass 100g, choose the potato leaching powder of the 3g/L of 40g, the agar powder of the glucose of the 20g/L of 40g and the 15g/L of 20g makes primary culture medium, again by gross mass 100g, choose the potato leaching powder of the 5g/L of 25g, the peptone of the 10g/L of 25g, the sodium chloride of the glucose of the 15g/L of 25g and the 5g/L of 25g makes secondary medium; By 12 strain candidate lactic acid bacteria strains, 12 strain photosynthetic bacterias, the activated rear access primary culture medium of 12 strain chromic acid bacterium, its pH value and lactic bacteria strain number is measured respectively after Anaerobic culturel 30h, primary dcreening operation obtains producing acid and growing lactic acid bacteria strains faster, from photosynthetic bacteria and chromic acid bacteria strain, measure 4 the highest strain photosynthetic bacterias of bacterial strain and chromic acid bacteria strain, continued cultivation for subsequent use; Again saccharomycete is put Yeasts on starch culture-medium, after cultivating 21h, choose the highest yeast strain of 7 strain bacterial strains, continue to cultivate, for subsequent use; Again by the lactic acid bacteria of above-mentioned cultivation, saccharomycete, photosynthetic bacteria and chromic acid bacteria strain liquid by 10% inoculum concentration to be inoculated in pH be respectively on the secondary medium of 2, be in the secondary medium of the natrii tauroglycocholas of 0.4% by the inoculum concentration cut-in quality mark of 1% again after bacterium liquid being collected after quiescent culture 2h at 37 DEG C, can complete bacterial strain postsearch screening to its collection after quiescent culture 2h at 37 DEG C, for subsequent use; Finally make primary raw material by wheat bran, after wheat bran high-temperature sterilization, the ripe bacterium liquid of above-mentioned screening lactic acid bacteria for subsequent use, saccharomycete, photosynthetic bacteria and chromic acid bacterium is inoculated in the ratio of 3:3:3:1, total inoculum concentration is 10% of bran mass gross mass, to its anaerobic fermentation 2d at 28 DEG C, after fermentation completely at 23 DEG C low temperature drying 25h, a kind of microbiological feed improving efficiency of feed utilization can be obtained.
Added to by obtained microbiological feed in other roughage raw materials, addition is 75g/kg, and stir 13min, mix, throw something and feed, after 1 month, feed consumption decreases 6%, and efficiency of feed utilization reaches 96.2%, before not adding, improve 9%.
Example 3
First by gross mass 100g, choose the potato leaching powder of the 3g/L of 35g, the agar powder of the glucose of the 20g/L of 45g and the 15g/L of 20g makes primary culture medium, again by gross mass 100g, choose the potato leaching powder of the 5g/L of 30g, the peptone of the 10g/L of 20g, the sodium chloride of the glucose of the 15g/L of 30g and the 5g/L of 20g makes secondary medium; By 12 strain candidate lactic acid bacteria strains, 12 strain photosynthetic bacterias, the activated rear access primary culture medium of 12 strain chromic acid bacterium, its pH value and lactic bacteria strain number is measured respectively after Anaerobic culturel 36h, primary dcreening operation obtains producing acid and growing lactic acid bacteria strains faster, from photosynthetic bacteria and chromic acid bacteria strain, measure 4 the highest strain photosynthetic bacterias of bacterial strain and chromic acid bacteria strain, continued cultivation for subsequent use; Again saccharomycete is put Yeasts on starch culture-medium, after cultivating 24h, choose the highest yeast strain of 8 strain bacterial strains, continue to cultivate, for subsequent use; Again by the lactic acid bacteria of above-mentioned cultivation, saccharomycete, photosynthetic bacteria and chromic acid bacteria strain liquid by 10% inoculum concentration to be inoculated in pH be respectively on the secondary medium of 3, be in the secondary medium of the natrii tauroglycocholas of 0.4% by the inoculum concentration cut-in quality mark of 1% again after bacterium liquid being collected after quiescent culture 2h at 37 DEG C, can complete bacterial strain postsearch screening to its collection after quiescent culture 2h at 37 DEG C, for subsequent use; Finally make primary raw material by wheat bran, after wheat bran high-temperature sterilization, the ripe bacterium liquid of above-mentioned screening lactic acid bacteria for subsequent use, saccharomycete, photosynthetic bacteria and chromic acid bacterium is inoculated in the ratio of 3:3:3:1, total inoculum concentration is 10% of bran mass gross mass, to its anaerobic fermentation 3d at 30 DEG C, after fermentation completely at 25 DEG C low temperature drying 30h, a kind of microbiological feed improving efficiency of feed utilization can be obtained.
Added to by obtained microbiological feed in other roughage raw materials, addition is 100g/kg, and stir 15min, mix, throw something and feed, after 1 month, feed consumption decreases 8%, and efficiency of feed utilization reaches 97%, before not adding, improve 10%.
Claims (1)
1. improve a microbiological feed for efficiency of feed utilization, it is characterized in that concrete preparation process is:
(1) by mass percentage, choose the potato leaching powder solution of the 3g/L of 30 ~ 40%, the glucose solution of 20g/L of 30 ~ 45% and the agar powder solution of the 15g/L of 20 ~ 35% make primary culture medium, again by mass percentage, choose the potato leaching powder solution of the 5g/L of 25 ~ 30%, the peptone solution of the 10g/L of 20 ~ 25%, the glucose solution of 15g/L of 20 ~ 30% and the sodium chloride solution of the 5g/L of 20 ~ 30% make secondary medium;
(2) by 12 strain candidate lactic acid bacteria strains, 12 strain photosynthetic bacterias, the activated rear access primary culture medium of 12 strain chromic acid bacterium, its pH value and lactic bacteria strain number is measured respectively after Anaerobic culturel 24 ~ 36h, primary dcreening operation obtains producing acid and growing lactic acid bacteria strains faster, from photosynthetic bacteria and chromic acid bacteria strain, measure 4 the highest strain photosynthetic bacterias of bacterial strain and chromic acid bacteria strain, continued cultivation for subsequent use; Again saccharomycete is put Yeasts on starch culture-medium, after cultivating 18 ~ 24h, choose the highest yeast strain of 6 ~ 8 strain bacterial strains, continue to cultivate, for subsequent use;
(3) by the lactic acid bacteria of above-mentioned cultivation, saccharomycete, photosynthetic bacteria and chromic acid bacteria strain by 10% inoculum concentration to be inoculated in pH be respectively on the secondary medium of 2 ~ 3, be in the secondary medium of the natrii tauroglycocholas of 0.4% by the inoculum concentration cut-in quality mark of 1% again after bacterium liquid being collected after quiescent culture 2h at 37 DEG C, can complete bacterial strain postsearch screening to its collection after quiescent culture 2h at 37 DEG C, for subsequent use;
(4) primary raw material is made by wheat bran, after wheat bran high-temperature sterilization, the ripe bacterium liquid of above-mentioned screening lactic acid bacteria for subsequent use, saccharomycete, photosynthetic bacteria and chromic acid bacterium is inoculated in the ratio of 3:3:3:1, total inoculum concentration is 10% of bran mass gross mass, by its anaerobic fermentation 2 ~ 3d at 25 ~ 30 DEG C, after fermentation completely at 20 ~ 25 DEG C low temperature drying 20 ~ 30h, a kind of microbiological feed improving efficiency of feed utilization can be obtained.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107927449A (en) * | 2017-12-04 | 2018-04-20 | 李六秀 | The preparation method of microbial fermentation prawn feed |
Citations (3)
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| CN1176790A (en) * | 1996-09-13 | 1998-03-25 | 盖军 | Prepn. contg. effedive micorbial population |
| CN1324574A (en) * | 2000-05-18 | 2001-12-05 | 湖北省葛店开发区畅响生物工程园有限公司 | Farm animal feed additive of live bacteria |
| CN1640293A (en) * | 2005-01-05 | 2005-07-20 | 徐州苏福生物技术开发有限公司 | Composite microbial fodder additive |
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- 2015-09-11 CN CN201510576168.1A patent/CN105124166A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1176790A (en) * | 1996-09-13 | 1998-03-25 | 盖军 | Prepn. contg. effedive micorbial population |
| CN1324574A (en) * | 2000-05-18 | 2001-12-05 | 湖北省葛店开发区畅响生物工程园有限公司 | Farm animal feed additive of live bacteria |
| CN1640293A (en) * | 2005-01-05 | 2005-07-20 | 徐州苏福生物技术开发有限公司 | Composite microbial fodder additive |
Non-Patent Citations (1)
| Title |
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| 黄俊等: "新型微生物饲料添加剂的开发及应用效果研究", 《饲料工业》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107927449A (en) * | 2017-12-04 | 2018-04-20 | 李六秀 | The preparation method of microbial fermentation prawn feed |
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