CN102669409B - Method for preparing fermentation promoting peptide of fermented feed from mushroom residue - Google Patents

Abstract

The invention discloses a method for preparing fermented feed fermentation promoting peptide from mushroom residue by use of a double enzymolysis technology. The method comprises the following steps of: performing enzymolysis on the mushroom residue by use of cellulase and alkali protease so that the cell wall is softened and the soluble protein embedded in the cell wall is dissolved out to the greatest degree; and performing enzymolysis on the macromolecular compounds such as protein by use of alkali protease to obtain micromolecular fermentation promoting active peptide which contains polysaccharide and other biological active components. The mushroom residue has a fermentation promoting effect; through the transformation from the mushroom residue to the mushroom residue fermentation promoting peptide, the fermentation performance of lactic acid bacillus is enhanced, and the quality of the fermented feed is improved; and moreover, the method provides a new means for comprehensively utilizing the mushroom residue, changes the waste of mushroom residue into wealth, reduces environmental pollution, improves the production comprehensive utilization benefits of mushroom residue and has a broad prospect in market development.

Description

Prepare the method that fermented feed inspires the ferment peptide with the mushroom slag

Technical field

The present invention relates to a kind of is the method that the feedstock production fermented feed inspires the ferment peptide with the mushroom slag, belongs to the livestock feed technical field.

Background technology

Mushroom is second-biggest-in-the-world edible mushroom, also is one of China's special product, and at the title of among the people have " mountain delicacy ", it is a kind of fungi that is grown on the timber.Delicious flavour, the fragrance people that oozes is rich in nutriments such as a large amount of protein, B family vitamin, lentinan, has the raising body immunity, reduces effects such as blood fat and blood pressure, have " plant queen " good reputation.Owing to the multiple physiological active functions of lentinan, become the primary raw material of many health products and medicine, the production of industrialization lentinan at present is many carries precipitation with alcohol based on water, and the extensive water of lentinan extracts, and causes a large amount of mushroom dregs to produce.To the processing of mushroom slag, directly abandoned or the mode of burning changes to the direction of the comprehensive utilization of waste resource by original.Find still residual 17～18% protein, 15 seed amino acids, 7.32% nutriments such as total reducing sugar after the lixiviate of mushroom slag through chemical composition analysis.In recent years, the comprehensive utilization of mushroom slag was mainly reflected in: the one, be used for growing seedlings as biological organic matter; The 2nd, cultivate specified microorganisms as culture matrix; The 3rd, be used as the raw material of fermented feed.Though the adverse effect that the mushroom slag brings has been alleviated in these application to a certain extent, the benefit in actual production still loses significantly.

To be two or more amino acid link to each other and the compound of biologically active with peptide bond biologically active peptide.Active peptide has multiple organism metabolism and physiological regulation function, easily digest and assimilate, having effects such as promoting immunity, hormone are regulated, antiviral, hypotensive, reducing blood lipid, is the most popular research topic of current international food circle and the function factor that has development prospect.The production method of biologically active peptide mainly contains: separation and Extraction method, proteolysis method, chemical synthesis, method of gene recombination and enzymatic isolation method.At present enzymatic isolation method because have low price, security is good, technology is simple, and resource is very abundant and cheap, and carries out advantage such as suitability for industrialized production easily and be widely used in the actual production.Inspiring the ferment peptide is to have a class nutrition promoter of regulating microorganism metabolism, raising immunity of organisms, promotion growth and breeding, shortening fermentation period, but promotes the mechanism of fermentation also to it be unclear that.Disclose at present Semen phaseoli radiati polypeptide to lactobacillus-fermented and corn peptide to saccharomycetic fermentation, has obvious facilitation, the mushroom waste residue through the active peptide of enzymolysis processing preparation except peptide, also contain other active components such as lentinan, find first that through test the fermentation of the microorganism of lactic acid bacillus and other fermented feeds is had obvious facilitation, simultaneously to the quality that improves fermented feed with reduce production costs significant, therefore called after mushroom slag inspires the ferment peptide, does not still have the research report at present both at home and abroad.

Summary of the invention

The objective of the invention is to disclose the dual enzymolysis process of a kind of employing, prepare the method that fermented feed inspires the ferment peptide with the mushroom slag.

The present invention finishes by the following steps preparation: operating procedure of the present invention is as follows:

A. choose dry mushroom slag;

B. pulverize 80～100 mesh sieves and became the mushroom ground-slag;

C. add sterilized water in the mushroom ground-slag, its weight portion proportioning is: 20～30 parts of 50～100 parts of sterilized waters of mushroom ground-slag, form the mushroom dreg slurry with the abundant mixing of agitator, and the rotating speed of agitator is 300～600r/min;

D. the mushroom dreg slurry with mixing carries out homogeneous under flow 40～60L/h, pressure 30～50Mpa condition;

E. dual enzymolysis

E.1 behind homogeneous, add cellulase and carry out enzymolysis,

E.1.1 the vigor of cellulase is 20000U/g,

E.1.2 the enzyme addition is 300～400mg/L mushroom dreg slurry

E.1.3 temperature is 28 ℃～35 ℃,

E.1.4 adjust pH 4～5,

E.1.5 hydrolysis 0.5～1h,

E.2 carry out the alkali protease enzymolysis behind the cellulase degradation again,

E.2.1 the vigor of alkali protease is 25000U/g,

E.2.2 the enzyme addition is 200～250mg/L mushroom dreg slurry,

E.2.3 temperature is 40 ℃～50 ℃,

E.2.4 adjust pH 9～11,

E.2.5 hydrolysis 1～1.5h;

F. dry, packing,

F.1 the mushroom dreg slurry behind the enzymolysis adopts spraying or fluidized bed drying, airtight package,

G. make the mushroom slag and inspire the ferment peptide.

Effect of the present invention is recycling of waste resources, and raw material is easy to get, and technology is simple, is convenient to operation, both has been applicable to the production of industrialization industry, is applicable to that also the generation of common peasant household is used, and the actual production effect is obvious.Be to utilize cellulase and alkali protease that the mushroom waste residue is carried out enzymolysis, make softening of the cell wall, be embedded in soluble protein stripping to greatest extent in the cell membrane, with alkali protease macromolecular compounds such as protein are carried out enzymolysis then, obtain the micromolecular ferment active peptide that inspires, contain other biological active components such as polysaccharide simultaneously, for the comprehensive utilization of mushroom waste residue provides a new approach.The mushroom slag has the effect that inspires ferment, and the mushroom slag inspires the transformation of ferment peptide to the mushroom slag, has realized that the mushroom slag turns waste into wealth, and reduces and has eliminated environmental pollution hidden danger, improves the mushroom slag and produces comprehensive utilization benefit, has vast market prospect.

The specific embodiment

Now provide following specific embodiment, but the present invention is not limited to present embodiment:

A. choose dry mushroom waste residue;

B. pulverize 90 mesh sieves and became the mushroom ground-slag;

C. add sterilized water in the mushroom ground-slag, its weight portion proportioning is: 50 parts of mushroom ground-slags, and 25 parts of sterilized waters form the mushroom dreg slurry with the abundant mixing of agitator, and the rotating speed of agitator is 300r/min;

D. the mushroom dreg slurry with mixing carries out homogeneous under flow 50L/h, pressure 30Mpa condition;

E. dual enzymolysis

E.1 behind homogeneous, add cellulase and carry out enzymolysis,

E.1.1 the vigor of cellulase is 20000U/g,

E.1.2 the enzyme addition is 300mg/L mushroom dreg slurry,

E.1.3 temperature is 30 ℃,

E.1.4 adjust pH 4,

E.1.5 hydrolysis 0.5h,

E.2 carry out the alkali protease enzymolysis behind the cellulase degradation again,

E.2.1 the vigor of alkali protease is 25000U/g,

E.2.2 the enzyme addition is 250mg/L mushroom dreg slurry,

E.2.3 temperature is 50 ℃,

E.2.4 adjust pH 10,

E.2.5 hydrolysis 1h;

F. dry, packing,

F.1 80 ℃ of dryings on fluid bed of the slurries behind the enzymolysis, airtight package,

G. make the mushroom slag and inspire the ferment peptide.

The mushroom slag inspires the test of ferment and compares

Test objective: the one, the length of observing two groups of culture medium fermenting experiments, the 2nd, viable count in two groups of fermentation mediums is measured in the fermentation back.

1 materials and methods:

1.1 bacterial classification, 10 parts of lactic acid bacillus,

1.2 culture medium, the test group solid medium: 170 parts of corn flour, 200 parts in rice bran, 110 parts of dregs of beans, 300 parts in water, 50 parts in ammonium sulfate, mushroom slag inspire 150 parts of ferment peptides.

Control group solid medium: 170 parts of corn flour, 220 parts in rice bran, 110 parts of dregs of beans, 300 parts in water, 50 parts in ammonium sulfate.

1.3 fermentation condition is regulated 35 ℃ of fermentation temperatures, adjust pH 7.

1.4 experimental technique, fermentation back viable count adopts the method for plate culture count to measure.

2 results and analysis

2.1 the mushroom slag inspires the ferment peptide to the influence (seeing Table 1) of lactic acid bacillus fermentation back viable count

As shown in Table 1, with do not add the fermentation medium that the mushroom slag inspires the ferment peptide and compare, fermentation back mushroom slag inspires that viable count improves nearly 20 times in the fermentation medium of ferment peptide, this explanation mushroom slag peptide has tangible regulating action to growth, breeding and the metabolism of lactic acid bacillus, simultaneously fermented feed has high activity beneficial bacterium and active metabolite thereof, and the meat matter of palatability, trophism and livestock and poultry of improving feed is had positive effect.

2.2 the mushroom slag inspires the ferment peptide to the influence (seeing Table 2) of lactic acid bacillus fermentation time

As can be seen from Table 2, it is very significant to the influence of lactic acid bacillus fermentation time that the mushroom slag inspires the ferment peptide, the fermented feed required time of test group shortens 50% than control group, on the production technology of integral body, shortened the production cycle of fermented feed, reduce production cost, improved the added value of mushroom slag.

2.3 the mushroom slag inspires the comparison (seeing figure) that the ferment peptide inspires heat production aerogenesis in the ferment process

By Tu Kede, the time of heat production aerogenesis wants obviously preceding in control group to test group during the fermentation, illustrate that the mushroom slag inspires the ferment peptide and provides good material base for the growth and breeding of lactic acid bacillus, be conducive to the arrival of growth logarithmic phase, also shortened simultaneously the cycle that metabolite produces, reduce production cost, improved product competitiveness.

3 conclusions

The mushroom slag inspires in the fermentation medium of ferment peptide heat production aerogenesis 0.5～1d in advance, fermenting speed improves 1～1.5 times, and fermentation time shortens 1～1.5 times, has shortened the production cycle of fermented feed, reduced production cost, especially fermentation back viable count is by original 2.5 * 10 ⁸Be increased to 5.0 * 10 ⁹, total viable count increases about 20 times, has improved the quality of fermented feed.Therefore the mushroom slag inspires the ferment peptide and plays an important role for the fermenting property that improves lactic acid bacillus and the quality of improving fermented feed.

Claims (2)

1. prepare the method that fermented feed inspires the ferment peptide with the mushroom slag, it is characterized in that finishing by the following steps preparation:

A. choose dry mushroom slag;

B. pulverize 80～100 mesh sieves and became the mushroom ground-slag;

C. add sterilized water in the mushroom ground-slag, its weight portion proportioning is: 20～30 parts of 50～100 parts of sterilized waters of mushroom ground-slag, form the mushroom dreg slurry with the abundant mixing of agitator, and the rotating speed of agitator is 300～600r/min;

D. the mushroom dreg slurry with mixing carries out homogeneous under flow 40～60L/h, pressure 30～50Mpa condition;

E. dual enzymolysis

E.1 behind homogeneous, add cellulase and carry out enzymolysis,

E.1.1 the vigor of cellulase is 20000U/g,

E.1.2 the enzyme addition is 300～400mg/L mushroom dreg slurry

E.1.3 temperature is 28 ℃～35 ℃,

E.1.4 adjust pH 4～5,

E.1.5 hydrolysis 0.5～1h,

E.2 carry out the alkali protease enzymolysis behind the cellulase degradation again,

E.2.1 the vigor of alkali protease is 25000U/g,

E.2.2 the enzyme addition is 200～250mg/L mushroom dreg slurry,

E.2.3 temperature is 40 ℃～50 ℃,

E.2.4 adjust pH 9～11,

E.2.5 hydrolysis 1～1.5h;

F. dry, packing,

F.1 the mushroom dreg slurry behind the enzymolysis adopts spraying or fluidized bed drying, airtight package,

G. make the mushroom slag and inspire the ferment peptide.

2. according to claim 1ly prepare the method that fermented feed inspires the ferment peptide with the mushroom slag, it is characterized in that finishing by the following steps preparation:

A. choose dry mushroom waste residue;

B. pulverize 90 mesh sieves and became the mushroom ground-slag;

C. add sterilized water in the mushroom ground-slag, its weight portion proportioning is: 50 parts of mushroom ground-slags, and 25 parts of sterilized waters form the mushroom dreg slurry with the abundant mixing of agitator, and the rotating speed of agitator is 300r/min;

D. the mushroom dreg slurry with mixing carries out homogeneous under flow 50L/h, pressure 30Mpa condition;

E. dual enzymolysis

E.1 behind homogeneous, add cellulase and carry out enzymolysis,

E.1.1 the vigor of cellulase is 20000U/g,

E.1.2 the enzyme addition is 300mg/L mushroom dreg slurry,

E.1.3 temperature is 30 ℃,

E.1.4 adjust pH 4,

E.1.5 hydrolysis 0.5h,

E.2 carry out the alkali protease enzymolysis behind the cellulase degradation again,

E.2.1 the vigor of alkali protease is 25000U/g,

E.2.2 the enzyme addition is 250mg/L mushroom dreg slurry,

E.2.3 temperature is 50 ℃,

E.2.4 adjust pH 10,

E.2.5 hydrolysis 1h;

F. dry, packing,

F.1 80 ℃ of dryings on fluid bed of the slurries behind the enzymolysis, airtight package,

G. make the mushroom slag and inspire the ferment peptide.

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