CN107779414A - A kind of manioc waste fermentative microorganism microbial inoculum - Google Patents
A kind of manioc waste fermentative microorganism microbial inoculum Download PDFInfo
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- CN107779414A CN107779414A CN201610785647.9A CN201610785647A CN107779414A CN 107779414 A CN107779414 A CN 107779414A CN 201610785647 A CN201610785647 A CN 201610785647A CN 107779414 A CN107779414 A CN 107779414A
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- manioc waste
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
Abstract
The present invention relates to a kind of manioc waste fermentative microorganism microbial inoculum, is mixed by five kinds of aspergillus niger, candida utili, bacillus subtilis, bacillus licheniformis and lactobacillus acidophilus microorganisms.The beneficial effects of the invention are as follows：Microbial bacteria agent prescription of the present invention is reasonable, easy to use, and it can carry out high-efficiency fermenting to manioc waste, makes to effectively improve bioavilability of the manioc waste in animal containing a large amount of micro-organisms living cells, decomposition enzyme and other active components in feed.Not only there is the realistic meaning for solving problem of environmental pollution, and with the protein content in raising cassava dreg fodder, the actual application value of nutritive value, good precondition is created for manioc waste recycling.
Description
Technical field：
The present invention relates to a kind of manioc waste fermentative microorganism microbial inoculum, belong to technical field of microbial fermentation.
Background technology：
Manioc waste is to produce the waste material remained by tapioca, and there is individual starch and glucose producer more than 70 in Guangxi, had every year
About 70,000 tons of manioc waste (dry calculating), aboundresources；Manioc waste contain with cassava similar in substantial amounts of other nutritional ingredients,
Including a variety of trace elements beneficial to animal body.Manioc waste can not directly make feed because of its high humidity (moisture content is more than 60%)
Livestock and poultry are fed, and nutritive value is relatively low, and heap, which easily generates heat, to become sour, and causes environmental pollution.Manioc waste degraded and again profit
With a urgent need to solve the problem for having become solid waste management.
Had at this stage it is multi-party all ferment related problem studying relevant microorganism manioc waste, and occur a series of having
Close the microbial bacterial agent of microbial fermentation.But so far, drop of the existing microbial bacterial agent to lignin, cellulose in manioc waste
Solution rate is still relatively low, and the biological transformation ratio of the dead sample livestock of feed of production is not high.It would therefore be highly desirable to develop highly efficient manioc waste
Fermentative microorganism microbial inoculum, pollution of the manioc waste to environment is eliminated, improves the economic benefit of breeding enterprise, and Guangxi can be alleviated
Feedstuff present situation in short supply has important practical significance.
The content of the invention：
It is an object of the invention to provide a kind of manioc waste fermentation process and wood that can significantly improve manioc waste degradation effect
Potato slag fermentative microorganism microbial inoculum, to prepare manioc waste biological feedstuff.
The technical scheme that the present invention takes is as follows：
A kind of manioc waste fermentative microorganism microbial inoculum, by aspergillus niger, candida utili, bacillus subtilis, lichens gemma
Bacillus and lactobacillus acidophilus composition.
The manioc waste fermentative microorganism microbial inoculum each component volume fraction is：Aspergillus niger 20%, candida utili 20%,
Bacillus subtilis 20%, lichens gemma bar 20%, lactobacillus acidophilus 20%.
A kind of preparation method of the manioc waste fermentative microorganism microbial inoculum, including step is：
Step 1：Five kinds of strains are inoculated into activation culture in corresponding culture medium, aspergillus niger sabouraud's agar culture respectively
Base, candida utili corn Tween 80 agar medium, bacillus subtilis and bacillus licheniformis nutrient broth fine jade
Fat culture medium, lactobacillus acidophilus MRS agar mediums, respectively obtains generation bacterial strain.
Step 2：Five kinds of generation strains that step 1 is obtained, which are individually inoculated into nutrient broth agar culture medium, to be expanded
Culture obtains five kinds of bacterium solutions.
Step 3：Five kinds of bacterium solutions obtained by step 2 obtain the manioc waste fermentative microorganism after being mixed by volume equal proportion
Microbial inoculum.
28-32 DEG C of the step 1 activation culture temperature, incubation time 2-5 days.
It is 28-37 DEG C that the step 2, which expands cultivation temperature, incubation time 2-5 days.
A kind of manioc waste fermentation process, including step is：
(1) pretreatment of manioc waste：Manioc waste is mixed with appropriate corn flour, dregs of beans, system content moisture is existed
60%~70%, it is made and intends fermentation substrate；
(2) work seed bacterium solution：Microbial bacterial agent is inoculated into by every 100kg fermentation substrates with 0.15L dosage and contained
Activated in the mixed aqueous solution 1.0-1.5L of 0.85% salt and 1% glucose, work seed bacterium solution is made；
(3) mix and intend fermentation substrate：Above-mentioned work seed bacterium solution is well mixed with intending fermentation substrate, and plastic covering is thin
Film, when temperature is 25 DEG C~36 DEG C, ferment 3-5 days, when temperature is 10~20 DEG C, fermentation produces biological feedstuff in 5~10 days.
The weight of described plan fermentation substrate is than composition：Manioc waste 95%, corn flour 3%, dregs of beans 2%.
The advantageous effects of the present invention：The present invention is by aspergillus niger, candida utili, bacillus subtilis, lichens bud
Five kinds of microorganisms of spore bacillus and lactobacillus acidophilus mix.It can carry out high-efficiency fermenting to manioc waste, make to contain in feed
A large amount of micro-organisms living cells, enzyme and other active components are decomposed, effectively improve bioavilability of the manioc waste in animal.No
Only there is the realistic meaning for solving problem of environmental pollution, and with protein content, the nutriture value improved in cassava dreg fodder
The actual application value of value, good precondition is created for manioc waste recycling.
Embodiment：
In order that those skilled in the art more understand technical scheme and determine the technique effect of the present invention, first
Preferred embodiment is provided, but following preferred embodiments are not limited to or relied in the realization of the present invention.
Embodiment 1
A kind of manioc waste fermentative microorganism microbial inoculum, by aspergillus niger, candida utili, bacillus subtilis, lichens gemma
Bacillus and lactobacillus acidophilus composition.
The manioc waste fermentative microorganism microbial inoculum each component volume fraction is：Aspergillus niger 20%, candida utili 20%,
Bacillus subtilis 20%, lichens gemma bar 20%, lactobacillus acidophilus 20%.
A kind of preparation method of the manioc waste fermentative microorganism microbial inoculum, including step is：
Step 1：Five kinds of strains are inoculated into activation culture in corresponding culture medium, aspergillus niger sabouraud's agar culture respectively
Base, candida utili corn Tween 80 agar medium, bacillus subtilis and bacillus licheniformis nutrient broth fine jade
Fat culture medium, lactobacillus acidophilus MRS agar mediums, respectively obtains generation bacterial strain.
Step 2：Five kinds of generation strains that step 1 is obtained, which are individually inoculated into nutrient broth agar culture medium, to be expanded
Culture obtains five kinds of bacterium solutions.
Step 3：Five kinds of bacterium solutions obtained by step 2 obtain the manioc waste fermentative microorganism after being mixed by volume equal proportion
Microbial inoculum.
28 DEG C of the step 1 activation culture temperature, incubation time 2 days.
It is 28 DEG C that the step 2, which expands cultivation temperature, incubation time 2 days.
A kind of manioc waste fermentation process, including step is：
(1) pretreatment of manioc waste：Manioc waste is mixed with appropriate corn flour, dregs of beans, system content moisture is existed
60%, it is made and intends fermentation substrate；
(2) work seed bacterium solution：Microbial bacterial agent is inoculated into by every 100kg fermentation substrates with 0.15L dosage and contained
Activated in the mixed aqueous solution 1.0L of 0.85% salt and 1% glucose, work seed bacterium solution is made；
(3) mix and intend fermentation substrate：Above-mentioned work seed bacterium solution is well mixed with intending fermentation substrate, and plastic covering is thin
Film, when temperature is 25 DEG C~36 DEG C, ferment 3-5 days, when temperature is 10~20 DEG C, fermentation produces biological feedstuff in 5~10 days.
The weight of described plan fermentation substrate is than composition：Manioc waste 95%, corn flour 3%, dregs of beans 2%.
Manioc waste fermentative microorganism microbial inoculum described above and preparation method thereof and fermentation process, meet micro- life of the present invention
The proportion of agent, and the action effect of the microbial bacterial agent can be reached.
Embodiment 2
A kind of manioc waste fermentative microorganism microbial inoculum, by aspergillus niger, candida utili, bacillus subtilis, lichens gemma
Bacillus and lactobacillus acidophilus composition.
The manioc waste fermentative microorganism microbial inoculum each component volume fraction is：Aspergillus niger 20%, candida utili 20%,
Bacillus subtilis 20%, lichens gemma bar 20%, lactobacillus acidophilus 20%.
A kind of preparation method of the manioc waste fermentative microorganism microbial inoculum, including step is：
Step 1：Five kinds of strains are inoculated into activation culture in corresponding culture medium, aspergillus niger sabouraud's agar culture respectively
Base, candida utili corn Tween 80 agar medium, bacillus subtilis and bacillus licheniformis nutrient broth fine jade
Fat culture medium, lactobacillus acidophilus MRS agar mediums, respectively obtains generation bacterial strain.
Step 2：Five kinds of generation strains that step 1 is obtained, which are individually inoculated into nutrient broth agar culture medium, to be expanded
Culture obtains five kinds of bacterium solutions.
Step 3：Five kinds of bacterium solutions obtained by step 2 obtain the manioc waste fermentative microorganism after being mixed by volume equal proportion
Microbial inoculum.
32 DEG C of the step 1 activation culture temperature, incubation time 5 days.
It is 37 DEG C that the step 2, which expands cultivation temperature, incubation time 5 days.
A kind of manioc waste fermentation process, including step is：
(1) pretreatment of manioc waste：Manioc waste is mixed with appropriate corn flour, dregs of beans, system content moisture is existed
70%, it is made and intends fermentation substrate；
(2) work seed bacterium solution：Microbial bacterial agent is inoculated into by every 100kg fermentation substrates with 0.15L dosage and contained
Activated in the mixed aqueous solution 1.5L of 0.85% salt and 1% glucose, work seed bacterium solution is made；
(3) mix and intend fermentation substrate：Above-mentioned work seed bacterium solution is well mixed with intending fermentation substrate, and plastic covering is thin
Film, when temperature is 25 DEG C~36 DEG C, ferment 3-5 days, when temperature is 10~20 DEG C, fermentation produces biological feedstuff in 5~10 days.
The weight of described plan fermentation substrate is than composition：Manioc waste 95%, corn flour 3%, dregs of beans 2%.
Manioc waste fermentative microorganism microbial inoculum described above and preparation method thereof and fermentation process, meet micro- life of the present invention
The proportion of agent, and the action effect of the microbial bacterial agent can be reached.
Embodiment 3
A kind of manioc waste fermentative microorganism microbial inoculum, by aspergillus niger, candida utili, bacillus subtilis, lichens gemma
Bacillus and lactobacillus acidophilus composition.
The manioc waste fermentative microorganism microbial inoculum each component volume fraction is：Aspergillus niger 20%, candida utili 20%,
Bacillus subtilis 20%, lichens gemma bar 20%, lactobacillus acidophilus 20%.
A kind of preparation method of the manioc waste fermentative microorganism microbial inoculum, including step is：
Step 1：Five kinds of strains are inoculated into activation culture in corresponding culture medium, aspergillus niger sabouraud's agar culture respectively
Base, candida utili corn Tween 80 agar medium, bacillus subtilis and bacillus licheniformis nutrient broth fine jade
Fat culture medium, lactobacillus acidophilus MRS agar mediums, respectively obtains generation bacterial strain.
Step 2：Five kinds of generation strains that step 1 is obtained, which are individually inoculated into nutrient broth agar culture medium, to be expanded
Culture obtains five kinds of bacterium solutions.
Step 3：Five kinds of bacterium solutions obtained by step 2 obtain the manioc waste fermentative microorganism after being mixed by volume equal proportion
Microbial inoculum.
30 DEG C of the step 1 activation culture temperature, incubation time 3.5 days.
It is 32.5 DEG C that the step 2, which expands cultivation temperature, incubation time 3.5 days.
A kind of manioc waste fermentation process, including step is：
(1) pretreatment of manioc waste：Manioc waste is mixed with appropriate corn flour, dregs of beans, system content moisture is existed
65%, it is made and intends fermentation substrate；
(2) work seed bacterium solution：Microbial bacterial agent is inoculated into by every 100kg fermentation substrates with 0.15L dosage and contained
Activated in the mixed aqueous solution 1.25L of 0.85% salt and 1% glucose, work seed bacterium solution is made；
(3) mix and intend fermentation substrate：Above-mentioned work seed bacterium solution is well mixed with intending fermentation substrate, and plastic covering is thin
Film, when temperature is 25 DEG C~36 DEG C, ferment 3-5 days, when temperature is 10~20 DEG C, fermentation produces biological feedstuff in 5~10 days.
The weight of described plan fermentation substrate is than composition：Manioc waste 95%, corn flour 3%, dregs of beans 2%.
Manioc waste fermentative microorganism microbial inoculum described above and preparation method thereof and fermentation process, meet micro- life of the present invention
The proportion of agent, and the action effect of the microbial bacterial agent can be reached.
Embodiments of the invention are described in detail above, but the content is only presently preferred embodiments of the present invention,
It is not to be regarded as the practical range for limiting the present invention.Any changes and modifications in accordance with the scope of the present application,
Within the patent covering scope that the present invention all should still be belonged to.
Claims (7)
- A kind of 1. manioc waste fermentative microorganism microbial inoculum, it is characterised in that：By aspergillus niger, candida utili, bacillus subtilis, Bacillus licheniformis and lactobacillus acidophilus composition.
- 2. a kind of manioc waste fermentative microorganism microbial inoculum according to claim 1, it is characterised in that the manioc waste fermentation is micro- Bacteria agent each component volume fraction is：Aspergillus niger 20%, candida utili 20%, bacillus subtilis 20%, lichens bud Spore bar 20%, lactobacillus acidophilus 20%.
- 3. a kind of preparation method of manioc waste fermentative microorganism microbial inoculum according to claim 1, including step is：Step 1：Five kinds of strains are inoculated into activation culture in corresponding culture medium, aspergillus niger sabouraud's agar, production respectively Protein Candida corn Tween 80 agar medium, bacillus subtilis and bacillus licheniformis nutrient broth agar culture Base, lactobacillus acidophilus MRS agar mediums, respectively obtains generation bacterial strain；Step 2：Five kinds of generation strains that step 1 is obtained, which are individually inoculated into nutrient broth agar culture medium, expands culture Obtain five kinds of bacterium solutions；Step 3：Five kinds of bacterium solutions obtained by step 2 obtain the manioc waste fermentative microorganism bacterium after being mixed by volume equal proportion Agent.
- A kind of 4. preparation method of manioc waste fermentative microorganism microbial inoculum according to claim 3, it is characterised in that the step Rapid 28-32 DEG C of 1 activation culture temperature, incubation time 2-5 days.
- A kind of 5. preparation method of manioc waste fermentative microorganism microbial inoculum according to claim 3, it is characterised in that the step Rapid 2 expansion cultivation temperature is 28-37 DEG C, incubation time 2-5 days.
- 6. a kind of manioc waste fermentation process, including step is：(1) pretreatment of manioc waste：Manioc waste is mixed with appropriate corn flour, dregs of beans, make system content moisture 60%~ 70%, it is made and intends fermentation substrate；(2) work seed bacterium solution：Microbial bacterial agent is inoculated into containing 0.85% by every 100kg fermentation substrates with 0.15L dosage Activated in the mixed aqueous solution 1.0-1.5L of salt and 1% glucose, work seed bacterium solution is made；(3) mix and intend fermentation substrate：Above-mentioned work seed bacterium solution is well mixed with intending fermentation substrate, and plastic covering film, When temperature is 25 DEG C~36 DEG C, ferment 3-5 days, when temperature is 10~20 DEG C, fermentation produces biological feedstuff in 5~10 days.
- A kind of 7. manioc waste fermentation process according to claim 6, it is characterised in that the weight of described plan fermentation substrate It is than composition：Manioc waste 95%, corn flour 3%, dregs of beans 2%.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108850555A (en) * | 2018-08-06 | 2018-11-23 | 钟山县良丰生态农业有限公司 | A kind of manioc waste biological feedstuff and preparation method thereof |
| CN109749944A (en) * | 2019-03-11 | 2019-05-14 | 南京农业大学 | Complex micro organism fungicide and its preparation method and application for manioc waste substrate fermentation |
| CN110537632A (en) * | 2019-10-15 | 2019-12-06 | 中国热带农业科学院热带作物品种资源研究所 | Fermented cassava residue feed for southern barn-fed mutton sheep and preparation and application methods thereof |
| CN113545414A (en) * | 2021-08-11 | 2021-10-26 | 中国热带农业科学院广州实验站 | Biological feed and preparation method and application method thereof |
| CN114507659A (en) * | 2021-12-04 | 2022-05-17 | 广西壮族自治区农业科学院 | Preparation method of microbial agent carrier |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108850555A (en) * | 2018-08-06 | 2018-11-23 | 钟山县良丰生态农业有限公司 | A kind of manioc waste biological feedstuff and preparation method thereof |
| CN109749944A (en) * | 2019-03-11 | 2019-05-14 | 南京农业大学 | Complex micro organism fungicide and its preparation method and application for manioc waste substrate fermentation |
| CN110537632A (en) * | 2019-10-15 | 2019-12-06 | 中国热带农业科学院热带作物品种资源研究所 | Fermented cassava residue feed for southern barn-fed mutton sheep and preparation and application methods thereof |
| CN113545414A (en) * | 2021-08-11 | 2021-10-26 | 中国热带农业科学院广州实验站 | Biological feed and preparation method and application method thereof |
| CN114507659A (en) * | 2021-12-04 | 2022-05-17 | 广西壮族自治区农业科学院 | Preparation method of microbial agent carrier |
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