CN102934737A - Preparation method of protein feed - Google Patents
Preparation method of protein feed Download PDFInfo
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- CN102934737A CN102934737A CN2012105254698A CN201210525469A CN102934737A CN 102934737 A CN102934737 A CN 102934737A CN 2012105254698 A CN2012105254698 A CN 2012105254698A CN 201210525469 A CN201210525469 A CN 201210525469A CN 102934737 A CN102934737 A CN 102934737A
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Abstract
The invention is applicable to the field of biotechnology, and provides a preparation method of a protein feed. The method comprises the following steps: preparing fermentative strains, preparing a fermentative mold starter, and carrying out mixed fermentation by taking cassava dregs as the raw material to obtain a biological feed crude product; then mixing uniformly, pulverizing, screening, drying and packaging to obtain a biological feed finished product; adding a microbe strain into the cassava dregs, then fermenting to obtain the protein feed. The microbe stain is the combination of rhizopus R2, bacillus, microzyme and lactobacillus. According to the preparation method of the protein feed, cassava dregs are used as the raw materials, are low in cost and widely available, so that not only can the reuse of industrial residue be realized, and the problem of pollution of cassava dregs can be solved, but also the high-quality protein feed is obtained.
Description
Technical field
The present invention is applicable to the biotechnology field, especially a kind of preparation method of protein feed.
Background technology
Manioc waste is the waste material after tapioca or the alcohol processing, if it is entered rivers with sewage, can cause environmental pollution, often is used for originating as a kind of roughage of cheapness by plant because manioc waste is nontoxic.The main component of manioc waste is starch and cellulose, a small amount of protein, the raiser manioc waste with feed pigs after some feeds mix to boil, but feed pigs with manioc waste merely or manioc waste and feed hodgepodge are fed pigs, not only to the extremely low and poor growth of nutrition digestion absorptivity of manioc waste itself.If it is improper to store, manioc waste will go bad within a couple of days, caused easily poisoning.
Prior art discloses a kind of preparation method of the manioc waste fermentation compound for feeding the fowl poultry, and the method comprises:
1. prepare burden, each raw material weight percentage is respectively: manioc waste 67～73%, carbon source 7～15%, nitrogenous source 5～10%, molasses 7～15%, lactic acid bacteria 0.00019～0.00025%;
2. the rear formation compound that each raw material stirred is sent into the inherent anaerobic state bottom fermentation of container 48～96 hours to compound, and the pH value is controlled at 5～7 during fermentation, humidity is controlled at 50～70%rh, temperature is controlled at 30～40 ℃.
But the bacterial classification that prior art is selected is lower to cellulosic degradation effect in the manioc waste, or bacterial classification is not processed in detail, causes degradation effect lower.
Summary of the invention
For above problem, the invention provides a kind of preparation method of protein feed, with microorganism manioc waste is processed, make it be converted into the feed that protein content height, animal are easy to digest and assimilate.
For achieving the above object, the technical solution used in the present invention is: a kind of preparation method of protein feed, plant song and obtain the biological feedstuff crude product take manioc waste as the raw material mixed fungus fermentation by preparation fermented bacterium, preparation fermentation, again through evenly mix, pulverize, sieve, dry and packing can obtain the biological feedstuff finished product, microorganism fungus kind is added in the manioc waste, then obtain by fermentation protein feed, described microorganism fungus kind is the combination of head mold R2, bacillus, saccharomycete and lactic acid bacteria.
The mixed fungus fermentation process may further comprise the steps:
(1) prepare feedstuff: the component that feedstuff comprises and parts by weight thereof are: 500～800 parts of manioc wastes, 100～200 parts in wheat bran, 3～10 parts of dregs of beans, 1～2 part of ammonium chloride, 1～3 part in urea, 1～2 part of potassium dihydrogen phosphate; Mix adding in the manioc waste after wheat bran, dregs of beans, ammonium chloride, urea, the potassium dihydrogen phosphate mixing.
Wheat bran, i.e. wheat skin, the wheat processing milling by products, wheat is yellow, sheet or powdery.Be rich in cellulose and vitamin, that main application has is edible, be used as medicine, feedstuff, wine brewing etc.
Ammonium chloride is colourless crystallization or white crystalline powder; Odorless is distinguished the flavor of into, cold; Have draw moist.
Dregs of beans is a kind of byproduct that obtains behind the soybean extracting bean oil.Different according to the method for extracting, can be divided into and soak dregs of beans and two and soak two kinds of dregs of beans.Wherein the byproduct behind the extraction extracting bean oil soaks dregs of beans as one, and gets oil with squeezing first, gets behind the oil byproduct of gained through lixiviate again and is called two and soaks dregs of beans.In whole process, very important to the control of temperature, excess Temperature can have influence on protein content, thereby is directly connected to quality and the use of dregs of beans; Temperature is crossed the low water content that can increase dregs of beans, and the water content height then can affect the quality of dregs of beans in the storage life.One production technology of soaking dregs of beans is comparatively advanced, and protein content is high, is the principal item that circulates on the domestic present spot market.
Urea can the very effective protein denaturation that makes, and especially can very effectively destroy the protein of non-covalent bond combination.These characteristics can improve the solubility of some protein.
Potassium dihydrogen phosphate is industrial as buffer, nutrient chemical; Also as the synthetic flavor enhancement that spills clearly of casate, the raw material of potassium metaphosphate processed, the nutrient chemical of brewer's yeast, hardening agent, leavening agent, fermentation assistant.
(2) evenly admix 1～5 part of 1～8 part of the fermentation kind song of head mold R2 and saccharomycetic fermentation kind song in the feedstuff, 1～5 part of the fermentation kind song that adds two days later bacillus, again through after one day, 1～5 part of the liquid seeds of adding lactic acid bacteria, be to ferment under 25～40 ℃ in environment temperature, fermentation time is 1～3 day, obtains afterwards the biological feedstuff crude product.
Preparation fermented bacterium process may further comprise the steps:
(1) preparation liquid fermentation medium: head mold R2, bacillus and saccharomycetic liquid fermentation medium ingredient and parts by weight thereof are: 500～1500 parts in 1～5 part of soy peptone, 10～30 parts of glucose, 0.1～2 part of dipotassium hydrogen phosphate, 0.1～2 part in ammonium nitrate, 0.1～0.5 part of potassium dihydrogen phosphate, 0.1～0.6 part in magnesium sulfate and water; Liquid fermentation medium ingredient and the parts by weight thereof of lactic acid bacteria are: 500～1500 parts in 5～15 parts of glucose, 5～15 parts of yeast juices, 0.1～1 part of dipotassium hydrogen phosphate, 0.1～0.5 part in sodium chloride, 1～5 part in calcium carbonate, 0.1～0.5 part in magnesium sulfate and water.
(2) fermentation tank, liquid fermentation medium are sterilized, get 1～5 part of head mold R21～8 part, 1～5 part of bacillus, 1～5 part in saccharomycete and lactic acid bacteria and carry out strain fermentation in fermentation tank corresponding to access respectively, fermentation temperature is 25～40 ℃, mixing speed is 50～200 rev/mins, air mass flow is 1～1.5VVm, fermentation time is 18～36 hours, obtains respectively the liquid seeds of head mold R2, bacillus, saccharomycete and lactic acid bacteria, and wherein lactic acid bacteria carries out strain fermentation twice.
(3) preparation solid fermentation culture medium: get 50～100 parts in wheat bran, 10～20 parts of manioc wastes, 10～20 parts of dregs of beans, 50～100 parts in water, place fermentation tank to soak, mix dregs of beans and water, add again wheat bran, manioc waste, mix, carry out afterwards boiling, add again nutrient solution, just obtain the solid fermentation culture medium.Nutrient solution contains the composition of following parts by weight: 0.1～1 part of 1～5 part in ammonium sulfate, 1～5 part of potassium dihydrogen phosphate, 1～5 part of epsom salt, 1～3 part in calcium chloride, 0.1～1 part in copper sulphate, 0.1～1 part in sodium chloride, 10～30 parts of glucose and vitamin.
(4) fermentation tank, solid fermentation culture medium are sterilized, carry out fermented and cultured in the fermentation tank that head mold R2, bacillus and 1～5 part of access of saccharomycetic liquid seeds is corresponding respectively, fermentation temperature is 25～35 ℃, mixing speed is 15～25 rev/mins, air mass flow is 10～15m3/h, fermentation time is 25～35h, obtains respectively head mold R2, bacillus and saccharomycetic solid seed.
Preparation fermentation kind of a bent process may further comprise the steps:
(1) head mold R2, bacillus and saccharomycetic solid seed are piled up respectively cultivation as the song material, environment temperature remains on 29～31 ℃, and the accumulation incubation time is 14～30h.
(2) treat that bent material surface is little white and begin caking, it shakeout that make the moisturizing poromeric material of sterilizing on its loose bonnet, moisture-heat preservation is cultivated 10～24h.The moisturizing poromeric material has wet straw mat, wet bamboo mat, wet sheet, ventilated membrane.
(3) treating that bent material covers with white hypha and caking is good, again it is shakeout, loose, it is divided into uniform fragment again, cover afterwards the moisturizing poromeric material, is that moisture-heat preservation is cultivated 5～10h in 25～28 ℃ the situation in environment temperature.
(4) remove the moisturizing poromeric material, ventilation dewetting, keeping room temperature is 25～40 ℃, continues to be cultured to kind of a bent maturation, it is bent to obtain respectively head mold R2, bacillus and saccharomycetic fermentation kind.
At last the biological feedstuff crude product is carried out product treatment, comprise mix, pulverize, sieve, dry and packing, just can obtain the biological feedstuff finished product.When feeding livestock and poultry, this feed can add auxiliary fodder, and auxiliary fodder is corn, dregs of beans, bone meal, fish meal, trace element, amino acid and vitamin.
The embodiment of the invention uses manioc waste to be raw material, and its cheap, wide material sources have not only been realized the recycling to industrial residue, have solved the pollution problem of manioc waste, but also has obtained high-quality protein feed.
Description of drawings
Fig. 1 is the preparation method's of the protein feed that provides of the embodiment of the invention process chart.
The specific embodiment:
In order to make purpose of the present invention, technical scheme and advantage clearer, below in conjunction with drawings and Examples, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, is not intended to limit the present invention.
Fig. 1 shows the preparation method's of the protein feed that the embodiment of the invention provides technological process, and details are as follows:
Head mold R2, bacillus and saccharomycete are carried out respectively liquid fermentation and culture, and add liquid seeds.Carrying out afterwards solid fermentation cultivates.Add the long-pending cultivation of the laggard windrow of solid seed, by fermentation kind of a Qu Jinhang mixed fungus fermentation, need to add feedstuff.After the lactic acid bacteria process liquid fermentation and culture first time, again carry out carrying out mixed fungus fermentation after liquid fermentation and culture adds liquid seeds.Carry out at last product treatment and obtain biological feedstuff.
Embodiment one:
1, preparation fermented bacterium:
Head mold R2, bacillus and saccharomycetic liquid fermentation medium prescription:
500 parts in 1 part of soy peptone, 10 parts of glucose, 0.1 part of dipotassium hydrogen phosphate, 0.1 part in ammonium nitrate, 0.1 part of potassium dihydrogen phosphate, 0.1 part in magnesium sulfate and water.
The liquid fermentation medium prescription of lactic acid bacteria:
500 parts in 5 parts of glucose, 5 parts of yeast juices, 0.1 part of dipotassium hydrogen phosphate, 0.1 part in sodium chloride, 1 part in calcium carbonate, 0.1 part in magnesium sulfate and water.The liquid fermentation medium of head mold R2, bacillus, saccharomycete and lactic acid bacteria is put into respectively fermentation tank 1～No. 4, fermentation tank, liquid fermentation medium are sterilized, get 1 part of head mold R21 part, 1 part of bacillus, 1 part in saccharomycete and lactic acid bacteria and carry out strain fermentation in fermentation tank corresponding to access respectively, head mold R2 fermentation temperature is 25 ℃, mixing speed is 50 rev/mins, air mass flow is 1VVm, and fermentation time is 36 hours; The fermentation of bacillus temperature is 35 ℃, and mixing speed is 100 rev/mins, and air mass flow is 1.5VVm, and fermentation time is 30 hours; The saccharomycetes to make fermentation temperature is 40 ℃, and mixing speed is 150 rev/mins, and air mass flow is 1.3VVm, and fermentation time is 24 hours; Lactic acid bacteria carries out fermenting twice, and fermentation temperature is 36 ℃ for the first time, and mixing speed is 150 rev/mins, air mass flow is 1.4VVm, and fermentation time is 18 hours, and fermentation temperature is 28 ℃ for the second time, mixing speed is 200 rev/mins, and air mass flow is 1.2VVm, and fermentation time is 20 hours.Obtain respectively thus the liquid seeds of head mold R2, bacillus, saccharomycete and lactic acid bacteria.
Get 50 parts in wheat bran, 80 parts of manioc wastes, 10 parts of dregs of beans, 40 parts in water, place fermentation tank to soak, mix dregs of beans and water, add again wheat bran, manioc waste, mix, carry out afterwards boiling, add nutrient solution, nutrient solution ingredient and parts by weight thereof are again: 0.1 part of 1 part in ammonium sulfate, 1 part of potassium dihydrogen phosphate, 1 part of epsom salt, 1 part in calcium chloride, 0.1 part in copper sulphate, 0.1 part in sodium chloride, 10 parts of glucose and vitamin obtain the solid fermentation culture medium thus.
Fermentation tank, solid fermentation culture medium are sterilized, carry out fermented and cultured in the fermentation tank that each 1 part access of head mold R2, bacillus and saccharomycetic liquid seeds is corresponding respectively, fermentation temperature is 25 ℃, and mixing speed is 15 rev/mins, and air mass flow is 10m
3/ h, fermentation time are 25 hours, thereby obtain respectively head mold R2, bacillus and saccharomycetic solid seed.
2, preparation fermentation kind of song:
The solid seed of head mold R2 is piled up cultivation as the song material, and environment temperature remains on 29 ℃, and the accumulation incubation time is 30h; Treat that bent material surface is little white and begin caking, it shakeout that make the wet straw mat of sterilization on its loose bonnet, moisture-heat preservation is cultivated 24h; Treating that bent material covers with white hypha and caking is good, again it is shakeout, loose, it is divided into uniform fragment again, cover afterwards wet straw mat, is that moisture-heat preservation is cultivated 10h in 25 ℃ the situation in environment temperature; Burring seat afterwards, ventilation dewetting, keeping room temperature is 25 ℃, continues to be cultured to kind bent ripe, the fermentation kind that obtains head mold R2 is bent.The solid seed of bacillus is piled up cultivation as the song material, and environment temperature remains on 31 ℃, and the accumulation incubation time is 14h; Treat that bent material surface is little white and begin caking, it shakeout that make the wet straw mat of sterilization on its loose bonnet, moisture-heat preservation is cultivated 10h; Treating that bent material covers with white hypha and caking is good, again it is shakeout, loose, it is divided into uniform fragment again, cover afterwards wet straw mat, is that moisture-heat preservation is cultivated 5h in 28 ℃ the situation in environment temperature; Burring seat afterwards, ventilation dewetting, keeping room temperature is 40 ℃, continues to be cultured to kind bent ripe, the fermentation kind that obtains bacillus is bent.
Saccharomycetic solid seed is piled up cultivation as the song material, and environment temperature remains on 30 ℃, and the accumulation incubation time is 20h; Treat that bent material surface is little white and begin caking, it shakeout that make the wet straw mat of sterilization on its loose bonnet, moisture-heat preservation is cultivated 16h; Treating that bent material covers with white hypha and caking is good, again it is shakeout, loose, it is divided into uniform fragment again, cover afterwards wet straw mat, is that moisture-heat preservation is cultivated 8h in 26 ℃ the situation in environment temperature; Burring seat afterwards, ventilation dewetting, keeping room temperature is 30 ℃, continues to be cultured to kind bent ripe, it is bent to obtain saccharomycetic fermentation kind.
3, mixed fungus fermentation:
Prepare feedstuff, the component that feedstuff comprises and parts by weight thereof are: 700 parts of manioc wastes, 100 parts in wheat bran, 3 parts of dregs of beans, 1 part of ammonium chloride, 1 part in urea, 1 part of potassium dihydrogen phosphate; Mix adding in the manioc waste after wheat bran, dregs of beans, ammonium chloride, urea, the potassium dihydrogen phosphate mixing.
Evenly admix bent each 1 part of head mold R2 and saccharomycetic fermentation kind in the feedstuff, it is shakeout accumulation, allow kind of Qu Shengchang two days later, add bent 1 part of the fermentation kind of bacillus, mix thoroughly, shakeout, pile up, again through after one day, 1 part of the liquid seeds of adding lactic acid bacteria, be to ferment under 25 ℃ in environment temperature, fermentation time is 1 day, obtains afterwards the biological feedstuff crude product.
After testing, contain a large amount of enzymes such as glucose restriction endonuclease, glucose excision enzyme, β～dextranase, pectase, zytase, phytase, lipase and protease in the biological feedstuff crude product; Contain the viable bacterias such as yeast, gemma, lactic acid bacteria, total plate count is about 1,000,000,000 CFU/g; Contain total amount and be about organic acid between carbon 1～carbon 7 of 10～20%.
At last the biological feedstuff crude product is carried out product treatment, comprise mix, pulverize, sieve, dry and packing, just can obtain the biological feedstuff finished product.When feeding livestock and poultry, this feed can add auxiliary fodder, and auxiliary fodder is corn, dregs of beans, bone meal, fish meal, trace element, amino acid and vitamin.
Table 1: amino acid whose content (g/100g) before and after the manioc waste fermentation
2: basic nutrition index before and after the fermentation of manioc waste compound
| Basic nutrition | Before the fermentation | After the fermentation |
| Crude fibre | ≥35％ | ≤15％ |
| Crude protein | ≤2％ | ≥9.5％ |
Data from upper table can find out that the Feed Energy that adopts preparation method of the present invention to make reduces crude fiber content, improves crude protein content, thereby improve manioc waste compound nutritive value.
Embodiment two:
1, preparation fermented bacterium:
Head mold R2, bacillus and saccharomycetic liquid fermentation medium prescription:
1500 parts in 5 parts of soy peptones, 30 parts of glucose, 2 parts of dipotassium hydrogen phosphates, 2 parts in ammonium nitrate, 0.5 part of potassium dihydrogen phosphate, 0.6 part in magnesium sulfate and water.
The liquid fermentation medium prescription of lactic acid bacteria:
1500 parts in 15 parts of glucose, 15 parts of yeast juices, 1 part of dipotassium hydrogen phosphate, 0.5 part in sodium chloride, 5 parts in calcium carbonate, 0.5 part in magnesium sulfate and water.
The liquid fermentation medium of head mold R2, bacillus, saccharomycete and lactic acid bacteria is put into respectively fermentation tank 1～No. 4, fermentation tank, liquid fermentation medium are sterilized, get 5 parts of head mold R28 part, 5 parts of bacillus, 5 parts in saccharomycete and lactic acid bacterias and carry out strain fermentation in fermentation tank corresponding to access respectively, head mold R2 fermentation temperature is 40 ℃, mixing speed is 50 rev/mins, air mass flow is 1.5VVm, and fermentation time is 30 hours; The fermentation of bacillus temperature is 34 ℃, and mixing speed is 100 rev/mins, and air mass flow is 1VVm, and fermentation time is 24 hours; The saccharomycetes to make fermentation temperature is 30 ℃, and mixing speed is 150 rev/mins, and air mass flow is 1.2VVm, and fermentation time is 20 hours; Lactic acid bacteria carries out fermenting twice, and fermentation temperature is 29 ℃ for the first time, and mixing speed is 150 rev/mins, air mass flow is 1.4VVm, and fermentation time is 20 hours, and fermentation temperature is 30 ℃ for the second time, mixing speed is 200 rev/mins, and air mass flow is 1.1VVm, and fermentation time is 24 hours.Obtain respectively thus the liquid seeds of head mold R2, bacillus, saccharomycete and lactic acid bacteria.
Get 100 parts in wheat bran, 20 parts of manioc wastes, 20 parts of dregs of beans, 100 parts in water, place fermentation tank to soak, mix dregs of beans and water, add again wheat bran, manioc waste, mix, carry out afterwards boiling, add nutrient solution, nutrient solution ingredient and parts by weight thereof are again: 1 part of 5 parts in ammonium sulfate, 5 parts of potassium dihydrogen phosphates, 5 parts of epsom salts, 3 parts in calcium chloride, 1 part in copper sulphate, 1 part in sodium chloride, 30 parts of glucose and vitamin obtain the solid fermentation culture medium thus.
Fermentation tank, solid fermentation culture medium are sterilized, carry out fermented and cultured in the fermentation tank that each 5 parts accesses of head mold R2, bacillus and saccharomycetic liquid seeds are corresponding respectively, fermentation temperature is 35 ℃, and mixing speed is 25 rev/mins, and air mass flow is 15m
3/ h, fermentation time are 35 hours, thereby obtain respectively head mold R2, bacillus and saccharomycetic solid seed.
2, preparation fermentation kind of song:
The solid seed of head mold R2 is piled up cultivation as the song material, and environment temperature remains on 29 ℃, and the accumulation incubation time is 30h; Treat that bent material surface is little white and begin caking, it shakeout that make the wet bamboo mat of sterilization on its loose bonnet, moisture-heat preservation is cultivated 24h; Treating that bent material covers with white hypha and caking is good, again it is shakeout, loose, it is divided into uniform fragment again, cover afterwards wet bamboo mat, is that moisture-heat preservation is cultivated 10h in 25 ℃ the situation in environment temperature; Remove afterwards bamboo mat, ventilation dewetting, keeping room temperature is 25 ℃, continues to be cultured to kind of a bent maturation, the fermentation kind that obtains head mold R2 is bent.
The solid seed of bacillus is piled up cultivation as the song material, and environment temperature remains on 31 ℃, and the accumulation incubation time is 14h; Treat that bent material surface is little white and begin caking, it shakeout that make the wet bamboo mat of sterilization on its loose bonnet, moisture-heat preservation is cultivated 10h; Treating that bent material covers with white hypha and caking is good, again it is shakeout, loose, it is divided into uniform fragment again, cover afterwards wet bamboo mat, is that moisture-heat preservation is cultivated 5h in 28 ℃ the situation in environment temperature; Remove afterwards bamboo mat, ventilation dewetting, keeping room temperature is 35 ℃, continues to be cultured to kind of a bent maturation, the fermentation kind that obtains bacillus is bent.
Saccharomycetic solid seed is piled up cultivation as the song material, and environment temperature remains on 30 ℃, and the accumulation incubation time is 20h; Treat that bent material surface is little white and begin caking, it shakeout that make the wet bamboo mat of sterilization on its loose bonnet, moisture-heat preservation is cultivated 16h; Treating that bent material covers with white hypha and caking is good, again it is shakeout, loose, it is divided into uniform fragment again, cover afterwards wet bamboo mat, is that moisture-heat preservation is cultivated 8h in 26 ℃ the situation in environment temperature; Remove afterwards bamboo mat, ventilation dewetting, keeping room temperature is 30 ℃, continues to be cultured to kind of a bent maturation, it is bent to obtain saccharomycetic fermentation kind.
3, mixed fungus fermentation:
Prepare feedstuff, the component that feedstuff comprises and parts by weight thereof are: 800 parts of manioc wastes, 200 parts in wheat bran, 10 parts of dregs of beans, 2 parts of ammonium chlorides, 3 parts in urea, 2 parts of potassium dihydrogen phosphates; Mix adding in the manioc waste after wheat bran, dregs of beans, ammonium chloride, urea, the potassium dihydrogen phosphate mixing.
Evenly admix in feedstuff with bent 5 parts of saccharomycetic fermentation kind bent 8 parts of the fermentation kind of head mold R2, it is shakeout accumulation, allow kind of Qu Shengchang two days later, add bent 5 parts of the fermentation kind of bacillus, mix thoroughly, shakeout, pile up, again through after one day, 5 parts of the liquid seeds of adding lactic acid bacteria, be to ferment under 40 ℃ in environment temperature, fermentation time is 3 days, obtains afterwards the biological feedstuff crude product.
At last the biological feedstuff crude product is carried out product treatment, comprise mix, pulverize, sieve, dry and packing, just can obtain the biological feedstuff finished product.When feeding livestock and poultry, this feed can add auxiliary fodder, and auxiliary fodder is corn, dregs of beans, bone meal, fish meal, trace element, amino acid and vitamin.
Embodiment three:
1, preparation fermented bacterium:
Head mold R2, bacillus and saccharomycetic liquid fermentation medium prescription:
1000 parts in 3 parts of soy peptones, 20 parts of glucose, 1 part of dipotassium hydrogen phosphate, 1 part in ammonium nitrate, 0.3 part of potassium dihydrogen phosphate, 0.4 part in magnesium sulfate and water.
The liquid fermentation medium prescription of lactic acid bacteria:
1000 parts in 10 parts of glucose, 10 parts of yeast juices, 0.5 part of dipotassium hydrogen phosphate, 0.3 part in sodium chloride, 3 parts in calcium carbonate, 0.3 part in magnesium sulfate and water.
The liquid fermentation medium of head mold R2, bacillus, saccharomycete and lactic acid bacteria is put into respectively fermentation tank 1～No. 4, fermentation tank, liquid fermentation medium are sterilized, carry out strain fermentation in the fermentation tank with head mold R24 part, 2 parts of bacillus, 2 parts in saccharomycete and 2 parts of respectively access correspondences of lactic acid bacteria, head mold R2 fermentation temperature is 26 ℃, mixing speed is 50 rev/mins, air mass flow is 1.5VVm, and fermentation time is 30 hours; The fermentation of bacillus temperature is 34 ℃, and mixing speed is 100 rev/mins, and air mass flow is 1VVm, and fermentation time is 24 hours; The saccharomycetes to make fermentation temperature is 39 ℃, and mixing speed is 150 rev/mins, and air mass flow is 1.2VVm, and fermentation time is 20 hours; Lactic acid bacteria carries out fermenting twice, and fermentation temperature is 29 ℃ for the first time, and mixing speed is 150 rev/mins, air mass flow is 1.4VVm, and fermentation time is 20 hours, and fermentation temperature is 30 ℃ for the second time, mixing speed is 200 rev/mins, and air mass flow is 1.1VVm, and fermentation time is 24 hours.Obtain respectively thus the liquid seeds of head mold R2, bacillus, saccharomycete and lactic acid bacteria.
Get 80 parts in wheat bran, 10 parts of manioc wastes, 10 parts of dregs of beans, 80 parts in water, place fermentation tank to soak, mix dregs of beans and water, add again wheat bran, manioc waste, mix,, carry out afterwards boiling, add again nutrient solution, nutrient solution ingredient and parts by weight thereof are: 0.5 part of 3 parts in ammonium sulfate, 3 parts of potassium dihydrogen phosphates, 3 parts of epsom salts, 2 parts in calcium chloride, 0.5 part in copper sulphate, 0.5 part in sodium chloride, 20 parts of glucose and vitamin obtain the solid fermentation culture medium thus.
Fermentation tank, solid fermentation culture medium are sterilized, carry out fermented and cultured in the fermentation tank that each 3 parts accesses of head mold R2, bacillus and saccharomycetic liquid seeds are corresponding respectively, fermentation temperature is 30 ℃, and mixing speed is 20 rev/mins, and air mass flow is 10m
3/ h, fermentation time are 30 hours, thereby obtain respectively head mold R2, bacillus and saccharomycetic solid seed.
2, preparation fermentation kind of song:
The solid seed of head mold R2 is piled up cultivation as the song material, and environment temperature remains on 29 ℃, and the accumulation incubation time is 30h; Treat that bent material surface is little white and begin caking, it shakeout that make the wet sheet of sterilization on its loose bonnet, moisture-heat preservation is cultivated 24h; Treating that bent material covers with white hypha and caking is good, again it is shakeout, loose, it is divided into uniform fragment again, cover afterwards wet sheet, is that moisture-heat preservation is cultivated 10h in 25 ℃ the situation in environment temperature; Remove afterwards sheet, ventilation dewetting, keeping room temperature is 25 ℃, continues to be cultured to kind of a bent maturation, the fermentation kind that obtains head mold R2 is bent.
3, mixed fungus fermentation:
Prepare feedstuff, the component that feedstuff comprises and parts by weight thereof are: 650 parts of manioc wastes, 150 parts in wheat bran, 5 parts of dregs of beans, 1.5 parts of ammonium chlorides, 2 parts in urea, 1.5 parts of potassium dihydrogen phosphates; Mix adding in the manioc waste after wheat bran, dregs of beans, ammonium chloride, urea, the potassium dihydrogen phosphate mixing.
Evenly admix in feedstuff with bent 2 parts of saccharomycetic fermentation kind bent 4 parts of the fermentation kind of head mold R2, it is shakeout accumulation, allow kind of Qu Shengchang two days later, add bent 2 parts of the fermentation kind of bacillus, mix thoroughly, shakeout, pile up, again through after one day, 2 parts of the liquid seeds of adding lactic acid bacteria, be to ferment under 30 ℃ in environment temperature, fermentation time is 2 days, obtains afterwards the biological feedstuff crude product.
At last the biological feedstuff crude product is carried out product treatment, comprise mix, pulverize, sieve, dry and packing, just can obtain the biological feedstuff finished product.When feeding livestock and poultry, this feed can add auxiliary fodder, and auxiliary fodder is corn, dregs of beans, bone meal, fish meal, trace element, amino acid and vitamin.
The embodiment of the invention uses manioc waste to be raw material, and its cheap, wide material sources have not only been realized the recycling to industrial residue, have solved the pollution problem of manioc waste, but also has obtained high-quality protein feed.
The above only is preferred embodiment of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (9)
1. the preparation method of a protein feed is characterized in that, said method comprising the steps of:
Preparation fermented bacterium, preparation fermentation are planted bent and are obtained the biological feedstuff crude product take manioc waste as the raw material mixed fungus fermentation;
With described biological feedstuff crude product through evenly mix, pulverize, sieve, dry and be packaged to be the biological feedstuff finished product.
2. the method for claim 1 is characterized in that, the employed bacterial classification of described fermentation is the combination of head mold R2, bacillus, saccharomycete and lactic acid bacteria.
3. the method for claim 1 is characterized in that, described preparation fermented bacterium specifically may further comprise the steps:
(1) preparation liquid fermentation medium: head mold R2, bacillus and saccharomycetic liquid fermentation medium ingredient and parts by weight thereof are: 500～1500 parts in 1～5 part of soy peptone, 10～30 parts of glucose, 0.1～2 part of dipotassium hydrogen phosphate, 0.1～2 part in ammonium nitrate, 0.1～0.5 part of potassium dihydrogen phosphate, 0.1～0.6 part in magnesium sulfate and water; Liquid fermentation medium ingredient and the parts by weight thereof of lactic acid bacteria are: 500～1500 parts in 5～15 parts of glucose, 5～15 parts of yeast juices, 0.1～1 part of dipotassium hydrogen phosphate, 0.1～0.5 part in sodium chloride, 1～5 part in calcium carbonate, 0.1～0.5 part in magnesium sulfate and water;
(2) fermentation tank, liquid fermentation medium are sterilized, get 1～5 part of head mold R21～8 part, 1～5 part of bacillus, 1～5 part in saccharomycete and lactic acid bacteria and carry out strain fermentation in fermentation tank corresponding to access respectively, fermentation temperature is 25～40 ℃, mixing speed is 50～200 rev/mins, air mass flow is 1～1.5VVm, fermentation time is 18～36 hours, obtains respectively the liquid seeds of head mold R2, bacillus, saccharomycete and lactic acid bacteria;
(3) preparation solid fermentation culture medium: get 50～100 parts in wheat bran, 10～20 parts of manioc wastes, 10～20 parts of dregs of beans, 50～100 parts in water, place fermentation tank to soak, mix dregs of beans and water, add again wheat bran, manioc waste, mix, carry out afterwards boiling, add again nutrient solution, just obtain the solid fermentation culture medium;
(4) fermentation tank, solid fermentation culture medium are sterilized, carry out fermented and cultured in the fermentation tank that head mold R2, bacillus and 1～5 part of access of saccharomycetic liquid seeds is corresponding respectively, fermentation temperature is 25～35 ℃, and mixing speed is 15～25 rev/mins, and air mass flow is 10～15m
3/ h, fermentation time are 25～35h, obtain respectively head mold R2, bacillus and saccharomycetic solid seed.
4. method as claimed in claim 3 is characterized in that, the lactic acid bacteria described in the described step (2) carries out strain fermentation twice.
5. method as claimed in claim 3, it is characterized in that the nutrient solution described in the described step (3) contains the composition of following parts by weight: 0.1～1 part of 1～5 part in ammonium sulfate, 1～5 part of potassium dihydrogen phosphate, 1～5 part of epsom salt, 1～3 part in calcium chloride, 0.1～1 part in copper sulphate, 0.1～1 part in sodium chloride, 10～30 parts of glucose and vitamin.
6. the method for claim 1 is characterized in that, described preparation fermentation kind of a song specifically may further comprise the steps:
(1) head mold R2, bacillus and saccharomycetic solid seed are piled up respectively cultivation as the song material, environment temperature remains on 29～31 ℃, and the accumulation incubation time is 14～30h;
(2) treat that bent material surface is little white and begin caking, it shakeout that make the moisturizing poromeric material of sterilizing on its loose bonnet, moisture-heat preservation is cultivated 10～24h;
(3) treating that bent material covers with white hypha and caking is good, again it is shakeout, loose, it is divided into uniform fragment again, cover afterwards the moisturizing poromeric material, is that moisture-heat preservation is cultivated 5～10h in 25～28 ℃ the situation in environment temperature;
(4) remove the moisturizing poromeric material, ventilation dewetting, keeping room temperature is 25～40 ℃, continues to be cultured to kind of a bent maturation, it is bent to obtain respectively head mold R2, bacillus and saccharomycetic fermentation kind.
7. method as claimed in claim 6 is characterized in that, described moisturizing poromeric material comprises wet straw mat, wet bamboo mat, wet sheet and ventilated membrane.
8. the method for claim 1 is characterized in that, described mixed fungus fermentation specifically may further comprise the steps:
(1) prepare feedstuff: the component that feedstuff comprises and parts by weight thereof are: 500～800 parts of manioc wastes, 100～200 parts in wheat bran, 3～10 parts of dregs of beans, 1～2 part of ammonium chloride, 1～3 part in urea, 1～2 part of potassium dihydrogen phosphate; Mix adding in the manioc waste after wheat bran, dregs of beans, ammonium chloride, urea, the potassium dihydrogen phosphate mixing;
(2) evenly admix 1～5 part of 1～8 part of the fermentation kind song of head mold R2 and saccharomycetic fermentation kind song in the feedstuff, 1～5 part of the fermentation kind song that adds two days later bacillus, again through after one day, 1～5 part of the liquid seeds of adding lactic acid bacteria, be to ferment under 25～40 ℃ in environment temperature, fermentation time is 1～3 day, obtains afterwards the biological feedstuff crude product.
9. the method for claim 1 is characterized in that, described feed can add auxiliary fodder, and auxiliary fodder comprises corn, dregs of beans, bone meal, fish meal, trace element, amino acid and vitamin.
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