CN102599344A - Cassava residue fermented feed and preparation method thereof - Google Patents
Cassava residue fermented feed and preparation method thereof Download PDFInfo
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- CN102599344A CN102599344A CN2012101134875A CN201210113487A CN102599344A CN 102599344 A CN102599344 A CN 102599344A CN 2012101134875 A CN2012101134875 A CN 2012101134875A CN 201210113487 A CN201210113487 A CN 201210113487A CN 102599344 A CN102599344 A CN 102599344A
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Abstract
The invention provides a method for preparing cassava residue fermented feeds. Microorganisms adopted for fermentative degradation are combined bacterial strains comprising one, two or three of aspergillus niger, trichoderma viride, rhizopus R2; by using a fermented strain formula and a fermentation method provided by the invention, the protein content of cassava residues is obviously increased; and because in the process of mixed-strain fermentation, the microbial growth condition in a multi-bacteria system is superior to that in a single strain fermentation system, and the mixed strains have a high utilization ratio of nutritional ingredients of cassava residues, an effect caused by mixed-strain fermentation is better than an effect caused by single-strain fermentation, thereby achieving the purpose of mutually complementing the advantages of mycete and saccharomycetes and enhancing effectiveness through mixing. A cassava residue feed prepared by using the method disclosed by the invention is higher in protein content, and suitable to be used as a feed.
Description
Technical field
The present invention relates to the biofermentation field, relate to manioc waste fermented feed and preparation method thereof in particular.
Technical background
Manioc waste is to produce the remaining waste material of tapioca, and mainly by the skin of the outside brown of cassava, inner parenchymal tissue is formed with most of poisonous cyanogen glycoside material, and wooden crude fibre content is higher, also contains a spot of starch and crude protein.Crude fibre has stronger stability, and resolution ratio is lower, difficult treatment.Along with the continuous increase of tapioca product consumption, quantum of output, product adjunct one manioc waste of in time not handling also increases, and not only can cause bigger pollution to environment; Contained waste water also can cause than havoc vegetation, soil, and the poison gas meeting atmosphere pollution that produces in the process of rotting further brings healthy hidden danger to humans and animals, causes economic loss.The manioc waste degraded reaches and utilizes the difficult problem that urgent need solves that has become the solid waste management again.
Manioc waste lacks nutriments such as starch and fat, is generally considered to be bum material.Need rationally utilize through the biotechnology means, manioc waste will become a kind of potential resource.In recent years; A lot of scholar's research show: it is effective processing mode that screening microorganism and enzyme preparation are carried out the degraded of manioc waste refuse; Utilizing microbial fermentation degraded manioc waste, can accomplish harmless treatment on the one hand, also is a kind of inexpensive method that increases protein content on the other hand; Having the great development potentiality, also is effectively to utilize cellulosic a kind of approach.But the manioc waste water content is higher, grows microorganism easily, is difficult for preserving, and contains higher hydrogen cyanide after going bad, and causes that easily animal poisons or morbidity; Fresh manioc waste is directly fed and is raised, and crude fibre can reduce food digestion in the animal stomach, influences metabolism, and the animal palatability is poor.This also is a manioc waste utilization rate reason of lower always.
Summary of the invention
Discover that the microbial degradation process is the manioc waste degraded, improve the key link of value.The manioc waste degraded is generally considered to be a kind of simple chemical reaction, and in fact degradation process is that very complicated material is asked interaction.The condition that influences cellulose degradation mainly comprises microbe species and envirment factor (kind and the content that comprise temperature, humidity, pH value and additive).Wherein microbe species is biodegradable core, is the most basic, most important, the strongest in all influence factors; Particular content of the present invention is:
A kind of preparation method of manioc waste fermented feed, the microorganism that said fermentative degradation adopts is aspergillus niger, Trichoderma viride, head mold R
2In a kind of, two or three combination bacterial strain.
The microorganism that said fermentative degradation adopts is aspergillus niger, Trichoderma viride and head mold R
2The combination bacterial strain of three kinds of bacterium.
Said aspergillus niger, Trichoderma viride, head mold R
2The inoculating strain quantity ratio of combination be 1～3:1～3:1～3.
Said aspergillus niger, Trichoderma viride, head mold R
2The inoculating strain quantity of combination than for 1:1:1.
Said liquid spawn addition is a volume ratio 3%, and the nitrogenous source addition is 10%, and the pH value is 5～6, and 37 ℃ of fermentation temperatures, fermentation time are 4 days.
5. manioc waste fermented feed, for adopting above-mentioned method, the fermentation manioc waste for preparing is directly as feed, or directly as feedstuff preparation becoming feed.
Useful technique effect of the present invention is: adopt fermented bacterium prescription provided by the invention and fermentation process, the manioc waste protein content obviously improves; Mix the strain fermentation effect and be better than single strain fermentation; This is because during mixed fungus fermentation; The growth of microorganism situation is superior to single bacterium fermentation system in the many thalline system, and mixed bacterial classification is higher to the utilization rate of manioc waste nutritional labeling, plays the purpose of the mutual supplement with each other's advantages of mould and saccharomycete, synergistic blend; It is higher to adopt method of the present invention to obtain the manioc waste dietary protein level, is suitable as feed and uses.
The specific embodiment
The screening of embodiment 1 manioc waste fermented bacterium
Choose aspergillus niger, Trichoderma viride, head mold R respectively
2, put into, in the 1000ml triangular flask of dress liquid 200-250ml, to cultivate 12 hours for 32 ℃, nutrient solution is a glucose 2%, urea 0.5%, corn steep liquor 2.5%, K
2HPO
40.1%; Obtain generation bacterium liquid.
With above-mentioned aspergillus niger, Trichoderma viride, head mold R
2The generation bacterium liquid of preparation is put in the seed culture filling, cultivates 7-10 hour for 32 ℃, and nutrient solution is a hydrolysis sugar 2.5%, urea 0.5%, corn steep liquor 2.5%, K
2HPO
40.1%; Obtain second-generation bacterial liquid; The standard of qualified bacterial classification is: inoculum density is 10
8-10
9Individual/mL, size evenly is single or Eight characters arrangement, flushes.Obtain aspergillus niger strain liquid, Trichoderma viride bacterial classification liquid and head mold R
2Strain liquid.
Take by weighing 17g manioc waste and 3g dregs of beans and join in the triangular flask of 250ml, steam sterilization 20min under skilful pound high pressure obtains the cassava fermentation medium; Utilize aspergillus niger strain liquid (A group), Trichoderma viride bacterial classification liquid (B group) and head mold R
2Strain liquid (C group) carries out the various combination strain fermentation, observes microbial growth speed and measures the protein content of tunning.The determining the protein quantity result is as shown in table 1.
Crude protein content (%) behind the table 1 different strain combined fermentation
Can find out that from table the product albumen content of double-bacterium ferment obviously improves, three bacterium are higher than two bacterium, aspergillus niger, Trichoderma viride and head mold R again
2Combined protein content the highest (being 14.6%), and the speed of growth is very fast, behind 48h, can see white hypha and cover matrix.
Use the bacterial classification combination of being selected and test determined composition of raw materials, aspergillus niger, Trichoderma viride, head mold R
2Be that 1:1:1,1:2:2,1:3:3,2:1:2,2:2:3,2:3:l, 3:1:3,3:2:1,3:3:2,1:1:4,4:1:1,1:4:1 carry out fermentation test, and measure the protein content of tunning by 5% inoculum concentration and different inoculative proportions respectively.Concrete outcome is as shown in table 2.
Table 2 different strain kind amount fermentation back crude protein content (%)
Can find out by table 2, in the experiment of the best inoculative proportion that is carried out, as bacterial classification combination aspergillus niger+Trichoderma viride+head mold R
2Inoculative proportion when being 1:1:1, the crude protein content the highest (being 16.4%) of fermentation back product, and growth of microorganism is rapid.
According to The above results, the inoculative proportion of confirming the The Best Mixed strain fermentation is an aspergillus niger: Trichoderma viride: head mold R
2=1:1:1.
The investigation of the condition of embodiment 2 culture mediums
Adopt embodiment 1 described aspergillus niger: Trichoderma viride: head mold R
2The bacterium formula of liquid of=1:1:1, the bacterium liquid and preparation method thereof is identical with embodiment 1, adopts orthogonal table, investigates the influence to protein yield of liquid bacterium addition, nitrogenous source addition, fermentation temperature and fermentation time.Experimental design is as shown in table 3.
Table 3 orthogonal experiment influence factor and horizontal tabulate
Wherein, PH also is one of key factor that influences the growth of microorganism metabolism.But the good shock-absorbing capacity of some material helps to reduce the needs to PH control in the solid fermentation.So during solid fermentation, generally adopt initial p H value, sweat need not detect and control pH value usually.
Carry out repeated test 3 times by table 3 composition, the orthogonal experiment analysis result is shown in table 4, table 5.
Table 4 orthogonal experiment intuitive analysis table
Table 5 orthogonal experiment analysis of variance table
F(0.05)=3.49 F(0.1)=2.61
Can know that from Orthogonal experiment results intuitive analysis R value the factor that influences result of the test is followed successively by C>D>B>A, promptly be followed successively by fermentation temperature, fermentation time, nitrogenous source addition and liquid seeds addition to producing the crude protein influence factor.
Can find out A from the K value
2B
4C
3D
2The crude protein content of tunning is maximum, and promptly the liquid spawn addition is 3%, and the nitrogenous source addition is 20%, and 37 ℃ of fermentation temperatures, fermentation time are 4 days.But in the culture experiment in later stage, consider the problem of aquaculture cost, the nitrogenous source addition confirms as 10%, is optimum option.
Variance analysis through orthogonal experiment; Can find out: factor C (fermentation temperature) and factor D (fermentation time) be to the significant difference that influences of experimental result, and factor A (liquid seeds inoculum concentration) and factor B (nitrogenous source addition) to experimental result to influence difference not remarkable.
To adopt aspergillus niger: Trichoderma viride: head mold R
2=1:1:1 bacterial classification prescription bacterium liquid; The nitrogenous source addition confirms as 10%, and the liquid spawn addition is 3%, 37 ℃ of fermentation temperatures; Fermentation time is 4 days manioc waste fermented products that obtain; Sample presentation country feeding quality supervision and inspection center adopts automatic amino acid analyzer GB|T18246-20000 to analyze content of amino acids in the product of fermentation back, and the result is as shown in table 6.
Content of amino acids (g/100g) before and after the fermentation of table 6 manioc waste
From table 6, can find out; Manioc waste is through after the solid fermentation; Contained various amino acid contents all improve a lot, and wherein each kind must also be greatly improved by amino acid content, reach 1820% like methionine content raising amount; Lysine content has improved 484%, and histidine content has improved 1045%.
Claims (6)
1. the preparation method of a manioc waste fermented feed is characterized in that: the microorganism that said fermentative degradation adopts is aspergillus niger, Trichoderma viride, head mold R
2In a kind of, two or three combination bacterial strain.
2. the preparation method of manioc waste fermented feed according to claim 1 is characterized in that: the microorganism that said fermentative degradation adopts is aspergillus niger, Trichoderma viride and head mold R
2The combination bacterial strain of three kinds of bacterium.
3. the preparation method of manioc waste fermented feed according to claim 2 is characterized in that: said aspergillus niger, Trichoderma viride, head mold R
2The inoculating strain quantity ratio of combination be 1～3:1～3:1～3.
4. the preparation method of manioc waste fermented feed according to claim 3 is characterized in that: said aspergillus niger, Trichoderma viride, head mold R
2The inoculating strain quantity of combination than for 1:1:1.
5. according to the preparation method of the said manioc waste fermented feed of claim 4, it is characterized in that: said liquid spawn addition is a volume ratio 3%, and the nitrogenous source addition is 10%, and the pH value is 5～6, and 37 ℃ of fermentation temperatures, fermentation time are 4 days.
6. manioc waste fermented feed is characterized in that: adopt the described method of claim 1-5, the fermentation manioc waste for preparing is directly as feed, or directly as feedstuff preparation becoming feed.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102835551A (en) * | 2012-08-27 | 2012-12-26 | 广州市番禺区大川饲料有限公司 | High protein fermentation cassava preparation method |
| CN102934737A (en) * | 2012-12-10 | 2013-02-20 | 广西高源淀粉有限公司 | Preparation method of protein feed |
| CN103013961A (en) * | 2012-12-17 | 2013-04-03 | 华南理工大学 | Method for producing neutral protease and feed additive by using fermentation of manioc wastes |
| CN103918874A (en) * | 2014-04-11 | 2014-07-16 | 四川农业大学 | Method for improving quality of manioc wastes by using mixed fermentation technology |
| CN104904996A (en) * | 2014-03-13 | 2015-09-16 | 梁昌 | Biological feed with cassava residue as main raw material and preparation method thereof |
| CN105494918A (en) * | 2015-12-18 | 2016-04-20 | 广西壮族自治区水牛研究所 | Method for solid-state fermentation of cassava residuals achieved through combination of lactobacillus buchneri, aspergillus niger and lactobacillus plantarum |
| CN107509915A (en) * | 2017-08-25 | 2017-12-26 | 广西壮族自治区水牛研究所 | A kind of method for reducing manioc waste cyanide content |
| CN108850429A (en) * | 2018-06-27 | 2018-11-23 | 广西驰胜农业科技有限公司 | A kind of cassava combines cassava residue fermented feed and preparation method thereof |
| CN109221677A (en) * | 2018-08-31 | 2019-01-18 | 天峨县平昌生态农业有限公司 | The preparation method of cassava dreg fodder |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102835551A (en) * | 2012-08-27 | 2012-12-26 | 广州市番禺区大川饲料有限公司 | High protein fermentation cassava preparation method |
| CN102934737A (en) * | 2012-12-10 | 2013-02-20 | 广西高源淀粉有限公司 | Preparation method of protein feed |
| CN103013961A (en) * | 2012-12-17 | 2013-04-03 | 华南理工大学 | Method for producing neutral protease and feed additive by using fermentation of manioc wastes |
| CN104904996A (en) * | 2014-03-13 | 2015-09-16 | 梁昌 | Biological feed with cassava residue as main raw material and preparation method thereof |
| CN103918874A (en) * | 2014-04-11 | 2014-07-16 | 四川农业大学 | Method for improving quality of manioc wastes by using mixed fermentation technology |
| CN103918874B (en) * | 2014-04-11 | 2015-10-21 | 四川农业大学 | A kind of method utilizing mixed fungus fermentation technology to improve manioc waste quality |
| CN105494918A (en) * | 2015-12-18 | 2016-04-20 | 广西壮族自治区水牛研究所 | Method for solid-state fermentation of cassava residuals achieved through combination of lactobacillus buchneri, aspergillus niger and lactobacillus plantarum |
| CN107509915A (en) * | 2017-08-25 | 2017-12-26 | 广西壮族自治区水牛研究所 | A kind of method for reducing manioc waste cyanide content |
| CN107509915B (en) * | 2017-08-25 | 2020-07-28 | 广西壮族自治区水牛研究所 | Method for reducing cyanide content of cassava residue |
| CN108850429A (en) * | 2018-06-27 | 2018-11-23 | 广西驰胜农业科技有限公司 | A kind of cassava combines cassava residue fermented feed and preparation method thereof |
| CN109221677A (en) * | 2018-08-31 | 2019-01-18 | 天峨县平昌生态农业有限公司 | The preparation method of cassava dreg fodder |
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Application publication date: 20120725 |