CN102144713A - Cassava residue feedstuff recycling technology - Google Patents
Cassava residue feedstuff recycling technology Download PDFInfo
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- CN102144713A CN102144713A CN201010107505XA CN201010107505A CN102144713A CN 102144713 A CN102144713 A CN 102144713A CN 201010107505X A CN201010107505X A CN 201010107505XA CN 201010107505 A CN201010107505 A CN 201010107505A CN 102144713 A CN102144713 A CN 102144713A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a cassava residue feedstuff recycling technology, which comprises the steps of: screening out strains capable of degrading cellulose in cassava residue from bacteria and fungi; carrying out the mutagenesis on the strains by various physical and chemical manners, screening out strains with high yield of cellulose and mycoprotein to be used as production strains; determining optimal fermentation conditions, such as volume of addition, inoculum size, inoculation time, fermentation temperature, fermentation time, pH value, ventilatory capacity and the like, of each component in a liquid fermentation medium by the optimization and the investigation on the fermentation mediums and fermentation conditions of each strain; determining other additives, except the cassava residue, and the volumes of the addition thereof in a mother culture fermentation medium; determining optimal fermentation conditions, such as hybrid fermentation inoculation species, inoculum size, inoculation time, fermentation temperature, fermentation time and the like; and solving the problem of pollution of the cassava residue to ensure that the cassava residue is fermented to obtain the high-protein feedstuff easily absorbed by animals.
Description
Technical field
Present technique be applicable to utilize microbial fermentation technology with manioc waste as fermentation substrate, under the comprehensive function of multiple microorganism, change the physics of manioc waste, chemical property makes it become that animal absorbs easily and the field of industrialized production of the high protein feed that utilizes
Background technology
The part of can not utilizing after the cassava processing is called manioc waste, and its main component is a cellulose.Undressed manioc waste goes out of use owing to being difficult to directly be utilized by livestock and poultry.Along with the increase of processing capacity, manioc waste has become one of source of contaminated environment in recent years.
Environmental protection policy is listed in " national Eleventh-Five Year Plan ".
Summary of the invention
Utilization filters out from bacterium and fungi and can utilize cellulosic bacterial strain in the degraded manioc waste; Carry out mutagenesis through multiple modes such as physics and chemistry, filter out the high bacterial strain of cellulase and mycoprotein output as producing bacterial classification.By to liquid fermentation medium with plant the koji fermentation culture medium and optimal conditions of fermentation such as the addition of determining each composition in the liquid fermentation medium of solid fermentation Optimum of culture medium and fermentation condition, inoculum concentration, inoculation time, fermentation temperature, fermentation time, pH value, throughput;
Determine other adding ingredients and addition except that manioc waste in kind of the koji fermentation culture medium;
Determine mixed culture fermentation inoculation kind, inoculum concentration, inoculation time, fermentation temperature, fermentation time, etc. optimal conditions of fermentation;
The specific embodiment
This technological process comprises: frozen control is equipped with, plate isolation, F
1Inclined-plane seed, seed bottle, fermentation flask, the checking of tiring, produce shake bottle, shake-flask seed, first order seed (liquid seeds) preparation, the preparation of solid jar seed, plant bently prepare, the solid mixed fungus fermentation.
Frozen control is equipped with:
Also claim " glycerine pipe method ", be used for the preservation of bacterial classification.
Plate isolation:
The used bacterial classification of this technology is decomposition of cellulose bacterium and fungi and since microorganism store and fermentation production process in degenerate easily, therefore must separate and identify it, generally select evaluation with the CMC culture medium; Isolate the bigger bacterial strain of cellulose-decomposing ability and carry out pure culture.
F
1The inclined-plane seed
Preserve with " slant tube method " from the seed that plate isolation is come out, be called first generation seed and also claim F
1The inclined-plane seed is generally cultivated with the PDA slant medium.
The seed bottle
Also claim the liquid shaking bottle seed, do activation, enlarge and cultivate usefulness, be second generation seed.Culture medium is the PDA fluid nutrient medium.
The fermentation flask seed
Simulate fermentation and enlarge production, the checking that is used to tire, culture medium is the PDA fluid nutrient medium.
The checking of tiring
With the FPA enzyme is criterion, picks out the high F of yield of enzyme
1The inclined-plane seed is used for fermenting and producing as starting strain, and culture medium is the PDA fluid nutrient medium.
Bottle, shake-flask seed are shaken in production
No. 1 seed
4 ℃ of frozen bacterial classifications are transferred on fresh PDA slant medium, leave standstill cultivation 4-7d. in 30 ℃ of constant incubators and get this inclined-plane, add sterilized water, scrape spore with oese, through 4 layers of gauze filter bacterium liquid, pour into again in the triangular flask of sterilization that bead and sterilized water are housed, in 180r/min vibrate to microscopy be dispersal spore, promptly obtain monospore suspension.Behind the blood counting chamber counting, the spore amount 10 of regulating spore suspension with sterilized water
7Individual/ml.Get monospore suspension and join (PDA fluid nutrient medium) in the seed culture medium, the amount that makes the seed culture medium miospore is 10
6Individual/ml, in 180r/min, cultivate 30h in 30 ℃ of constant temperature shaking tables and promptly get shake-flask seed.
No. 2 seeds
Method is the same substantially, and 4 ℃ of frozen bacterial classifications are transferred on fresh PDA slant medium, leaves standstill in 30 ℃ of constant incubators to cultivate 3d, and mycelia is greyish white, and bacterium colony is light green and gets final product.Get cultured inclined-plane seed, add the 5ml sterilized water, vibration is eluted to spore in the aseptic triangular flask, and being diluted to spore concentration is 104/ml, in 180r/min, cultivates 24h in 30 ℃ of constant temperature shaking tables and promptly gets shake-flask seed.
No. 3 seeds
Sterile working inserts a ring inclined-plane seed in the 60mlPDA fluid nutrient medium, in 30 ℃, cultivate 24h in the 220r/min constant temperature shaking table and promptly get shake-flask seed.
No. 4 seeds
Sterile working inserts a ring inclined-plane seed in the 60mlPDA fluid nutrient medium, in 30 ℃, cultivate 18h in the 220r/min constant temperature shaking table and promptly get shake-flask seed.
The liquid seeds preparation
1 No. 1 seed first order seed tank fermentation technology flow processs
1.1 the cleaning of fermentation tank tank body
Fully wash tank skin or in tank body, fill with water with running water, open agitator, stirred 10-20 minute.Clean up the water in the drain tank of back
Wipes of alcohol cleaning of evaporator and dispensing valve with 75% are noted the cleaning at dead angle
Cleaning finishes, and installs tank body, checks whether the sealing property of fermentation tank is intact
1.2 the interpolation of culture medium
Prepared culture medium 8-10L is added in the jar
Culture medium prescription:
Soy peptone: 2g glucose: 20g
Dipotassium hydrogen phosphate: 1g ammonium nitrate: 1g
Potassium dihydrogen phosphate: 0.46g magnesium sulfate: 0.5g
Water 1000ml pH nature
1.3 sterilization
1.3.1 pipeline sterilization
1.3.1.1 tank body pipeline sterilization
Opening conduits steam inlet valve cuts out tank body pipeline intake valve, and condensed water in the opening conduits air outlet valve, emptying pipe is treated to transfer big intake valve after steam is evenly discharged, and turns air outlet valve down, sterilization 30min
1.3.2 chuck preheating
Open the chuck outlet valve, open the jacket steam valve, feed small amount of steam, the water in the emptying chuck
Closed clip cover outlet valve is driven big jacket steam valve, pre-thermal jacket to 90 ℃
1.3.3 medium sterilization
Close the chuck steam valve, open the tank body steam valve, feed steam, treat that jar an interior temperature rises to 121 ℃ after, keep 121 ℃ of the interior temperature of jar, sterilized 30 minutes
1.4 inoculation
Sterilize after 30 minutes, close the tank body steam valve, treat that the tank body temperature is cooled to about 30 ℃, insert the triangular flask seed
1.4.1 the access of triangular flask seed
Light the alcohol ring, turn on the inoculation mouth rapidly, under the flame of alcohol ring, open the triangle bottle stopper, insert triangular flask seed (1%)
1.5 fermenting and producing
Open chuck draining valve and steam valve, steam flow in the control chuck makes the tank body temperature maintenance about 30 ℃
Open paddle, 50 rev/mins of control mixing speeds
Open the logical people's filtrated air of compressed air, air flow control is between 1--1.5VVm
Fermenting and producing 30---36 hour
2 No. 2 seed first order seed tank fermentation technology flow processs
2.1 the cleaning of fermentation tank tank body
Fully wash tank skin or in tank body, fill with water with running water, open agitator, stirred 10-20 minute.Clean up the water in the drain tank of back
Wipes of alcohol cleaning of evaporator and dispensing valve with 75% are noted the cleaning at dead angle
Cleaning finishes, and installs tank body, checks whether the sealing property of fermentation tank is intact
2.2 the interpolation of culture medium
Prepared culture medium 8-10L is added in the jar
Culture medium prescription:
Soy peptone: 2g glucose: 20g
Dipotassium hydrogen phosphate: 1g ammonium nitrate: 1g
Potassium dihydrogen phosphate: 0.46g magnesium sulfate: 0.5g
Water 1000ml pH nature
2.3 sterilization
2.3.1 pipeline sterilization
2.3.1.1 tank body pipeline sterilization
Opening conduits steam inlet valve cuts out tank body pipeline intake valve, and condensed water in the opening conduits air outlet valve, emptying pipe is treated to transfer big intake valve after steam is evenly discharged, and turns air outlet valve down, sterilization 30min
2.3.2 chuck preheating
Open the chuck outlet valve, open the jacket steam valve, feed small amount of steam, the water in the emptying chuck
Closed clip cover outlet valve is driven big jacket steam valve, pre-thermal jacket to 90 ℃
2.3.3 medium sterilization
Close the chuck steam valve, open the tank body steam valve, feed steam, treat that jar an interior temperature rises to 121 ℃ after, keep 121 ℃ of the interior temperature of jar, sterilized 30 minutes
2.4 inoculation
Sterilize after 30 minutes, close the tank body steam valve, treat that the tank body temperature is cooled to about 30 ℃, insert the triangular flask seed
2.4.1 the access of triangular flask seed
Light the alcohol ring, turn on the inoculation mouth rapidly, under the flame of alcohol ring, open the triangle bottle stopper, insert triangular flask seed (1%)
2.5 fermenting and producing
Open chuck draining valve and steam valve, steam flow in the control chuck remains on about 30 ℃ the tank body temperature
Open paddle, 100 rev/mins of control mixing speeds
Open the logical people's filtrated air of compressed air, air flow control is between 1--1.5VVm
Fermenting and producing 24--30 hour
3 No. 3 seed first order seed tank fermentation technology flow processs
3.1 the cleaning of fermentation tank tank body
Fully wash tank skin or in tank body, fill with water with running water, open agitator, stirred 10-20 minute.Clean up the water in the drain tank of back
Wipes of alcohol cleaning of evaporator and dispensing valve with 75% are noted the cleaning at dead angle
Cleaning finishes, and installs tank body, checks whether the sealing property of fermentation tank is intact
3.2 the interpolation of culture medium
Prepared culture medium 8-10L is added in the jar
Culture medium prescription:
Soy peptone: 2g glucose: 20g
Dipotassium hydrogen phosphate: 1g ammonium nitrate: 1g
Potassium dihydrogen phosphate: 0.46g magnesium sulfate: 0.5g
Water 1000ml pH nature
3.3 sterilization
3.3.1 pipeline sterilization
3.3.1.1 tank body pipeline sterilization
Opening conduits steam inlet valve cuts out tank body pipeline intake valve, and condensed water in the opening conduits air outlet valve, emptying pipe is treated to transfer big intake valve after steam is evenly discharged, and turns air outlet valve down, sterilization 30min
3.3.2 chuck preheating
Open the chuck outlet valve, open the jacket steam valve, feed small amount of steam, the water in the emptying chuck
Closed clip cover outlet valve is driven big jacket steam valve, pre-thermal jacket to 90 ℃
3.3.3 medium sterilization
Close the chuck steam valve, open the tank body steam valve, feed steam, treat that jar an interior temperature rises to 121 ℃ after, keep 121 ℃ of the interior temperature of jar, sterilized 30 minutes
3.4 inoculation
Sterilize after 30 minutes, close the tank body steam valve, treat that the tank body temperature is cooled to about 30 ℃, insert the triangular flask seed
3.4.1 the access of triangular flask seed
Light the alcohol ring, turn on the inoculation mouth rapidly, under the flame of alcohol ring, open the triangle bottle stopper, insert triangular flask seed (1%)
3.5 fermenting and producing
Open chuck draining valve and steam valve, steam flow in the control chuck remains on about 30 ℃ the tank body temperature
Open paddle, 150 rev/mins of control mixing speeds
Open the logical people's filtrated air of compressed air, air flow control is between 1--1.5VVm
Fermenting and producing 20--24 hour
4 No. 4 seed first order seed tank fermentation technology flow processs
4.1 the cleaning of fermentation tank tank body
Fully wash tank skin or in tank body, fill with water with running water, open agitator, stirred 10-20 minute; Clean up the water in the drain tank of back
Wipes of alcohol cleaning of evaporator and dispensing valve with 75% are noted the cleaning at dead angle
Cleaning finishes, and installs tank body, checks whether the sealing property of fermentation tank is intact
4.2 the interpolation of culture medium
Prepared culture medium 10L is added in the jar
Culture medium prescription:
Glucose: 10g yeast juice: 10g
Dipotassium hydrogen phosphate: 0.5g sodium chloride: 0.2g
Calcium carbonate: 2g magnesium sulfate: 0.2g
Water 1000ml pH nature
4.3 sterilization
4.3.1 pipeline sterilization
4.3.1.1 tank body pipeline sterilization
Opening conduits steam inlet valve cuts out tank body pipeline intake valve, and condensed water in the opening conduits air outlet valve, emptying pipe is treated to transfer big intake valve after steam is evenly discharged, and turns air outlet valve down, sterilization 30min
4.3.2 chuck preheating
Open the chuck outlet valve, open the jacket steam valve, feed small amount of steam, the water in the emptying chuck
Closed clip cover outlet valve is driven big jacket steam valve, pre-thermal jacket to 90 ℃
4.3.3 medium sterilization
Close the chuck steam valve, open the tank body steam valve, feed steam, treat that jar an interior temperature rises to 121 ℃ after, keep 121 ℃ of the interior temperature of jar, sterilized 30 minutes
4.4 inoculation
Sterilize after 30 minutes, close the tank body steam valve, treat that the tank body temperature is cooled to about 30 ℃, insert the triangular flask seed
4.4.1 the access of triangular flask seed
Light the alcohol ring, turn on the inoculation mouth rapidly, under the flame of alcohol ring, open the triangle bottle stopper, insert triangular flask seed (1%)
4.5 fermenting and producing
Open chuck draining valve and steam valve, steam flow in the control chuck remains on about 30 ℃ the tank body temperature
Open paddle, 150 rev/mins of control mixing speeds
Open the logical people's filtrated air of compressed air, air flow control is between 1--1.5VVm
Fermenting and producing 18--20 hour
5 No. 4 seed secondary seed tank fermentation technology flow processs
5.1 the cleaning of fermentation tank tank body
Fully wash tank skin or in tank body, fill with water with running water, open agitator, stirred 10-20 minute; Clean up the water in the drain tank of back
Wipes of alcohol cleaning of evaporator and dispensing valve with 75% are noted the cleaning at dead angle
Cleaning finishes, and installs tank body, checks whether the sealing property of fermentation tank is intact
5.2 the interpolation of culture medium
Prepared culture medium 50-60L is added in the jar
Culture medium prescription:
Glucose: 10g yeast juice: 10g
Dipotassium hydrogen phosphate: 0.5g sodium chloride: 0.2g
Calcium carbonate: 2g magnesium sulfate: 0.2g
Water 1000ml pH nature
5.3 sterilization
5.3.1 pipeline sterilization
5.3.1.1 tank body pipeline sterilization
Opening conduits steam inlet valve cuts out tank body pipeline intake valve, and condensed water in the opening conduits air outlet valve, emptying pipe is treated to transfer big intake valve after steam is evenly discharged, and turns air outlet valve down, sterilization 30min
5.3.1.2 culture transferring pipe sterilization
Opening conduits steam inlet valve, guarantee steam do not enter regulate the first class seed pot jar under the prerequisite of first class seed pot at the bottom of three-way valve, open culture transferring pipe air outlet valve, condensed water in the emptying pipe, treat to transfer big intake valve after steam is evenly discharged, turn air outlet valve down, sterilization 30min
5.3.2 chuck preheating
Open the chuck outlet valve, open the jacket steam valve, feed small amount of steam, the water in the emptying chuck
Closed clip cover outlet valve is driven big jacket steam valve, pre-thermal jacket to 90 ℃
5.3.3 medium sterilization
Close culture transferring pipe air outlet valve, close the chuck steam valve, open the tank body steam valve, feed steam, treat that jar an interior temperature rises to 121 ℃ after, keep 121 ℃ of jar interior temperature, the 30min that sterilizes,
5.4 culture transferring
Sterilize after 30 minutes, close the tank body steam valve, open recirculated water, treat that the tank body temperature is cooled to about 30 ℃, opens compressed air, regulate tank pressure, make tank pressure less than the first order seed pressure tank, open first class seed pot discharging valve, open secondary seed jar charging valve, utilize pressure differential that seed is moved into, inoculum concentration 1%
5.5 fermenting and producing
Open chuck draining valve and steam valve, steam flow in the control chuck remains on about 30 ℃ the tank body temperature
Open paddle, 200 rev/mins of control mixing speeds
Open the logical people's filtrated air of compressed air, air stream is controlled between the 1--1.5VVm most
About fermenting and producing 20-24 hour
The preparation of solid seed
1 No. 1 seed solid tank fermentation technology flow processs
1.1 the cleaning of fermentation tank tank body
Fully wash tank skin or in tank body, fill with water with running water, open paddle, till cleaning up.Clean up the water in the drain tank of back
1.2 the interpolation of solid material
The solid material and the water that crush are added in the jar in following ratio
Wheat bran: 80% manioc waste: 10%
Dregs of beans: 10% material: water 1: 0.8
1.3 material processing method
In dregs of beans and water added jar, soaked 30 minutes-1 hour, stir and want uniformity, add wheat bran then and manioc waste stirs, generally mixing time is 40 minutes, closes charging aperture after stirring
1.4 material boiling
1.4.1 chuck preheating
Open the chuck outlet valve, open the jacket steam valve, feed small amount of steam, the water in the emptying chuck
Closed clip cover outlet valve is driven big jacket steam valve, pre-thermal jacket to 90 ℃
1.4.2 material boiling and sterilization
Close the chuck steam valve, open the tank body steam valve, feed steam
Pressure cooking treats that generally pressure is that 0.15MP, temperature are about 121 ℃, and boiling 30 minutes picks up counting
1.5 pipeline sterilization
Comprise that inoculation pipeline and nutrient solution add the pipeline sterilization
Opening conduits steam inlet valve cuts out tank body pipeline intake valve, the opening conduits air outlet valve, and condensed water in the emptying pipe treats that steam evenly arranges
Go out the back and transfer big intake valve, turn air outlet valve down, sterilization 30min
1.6 inoculation
After the material boiling is good, open compressed air, open chuck recirculated water, be cooled to room temperature rapidly
Open the liquid tank bleeder valve, utilize pressure differential that liquid seeds is sucked the solid jar, inoculum concentration is 4%
1.7 nutrient solution adds
1.7.1 nutrient solution preparation
Press following formulated nutrient solution:
Ammonium sulfate 3g potassium dihydrogen phosphate 3g
Epsom salt 3g calcium chloride 1.5g
Copper sulphate 0.5g sodium chloride 0.5g
Glucose 20g vitamin 0.5g
1.7.2 nutrient solution sterilization
Above-mentioned nutrient solution is configured the 115 ℃ of sterilizations of replenisher tank 10--15 minute of packing into by solid dry material 1%
After treating its cooling, open the replenisher tank bleeder valve, utilize pressure differential that nutrient solution is sucked the solid jar
1.8 fermenting and producing
Open chuck draining valve and steam valve, steam flow in the control chuck remains on about 30 ℃ the tank body temperature
Open paddle, 20 rev/mins of control mixing speeds (intermittently stirring)
Open the logical people's filtrated air of compressed air, air flow control is at 10-15m
3/ h
About fermenting and producing 30 hours
2 No. 2 seed solid tank fermentation technology flow processs
2.1 the cleaning of fermentation tank tank body
Fully wash tank skin or in tank body, fill with water with running water, open paddle, stirred 10-20 minute.Clean up the water in the drain tank of back
2.2 the interpolation of solid material
The solid material and the water that crush are added in the jar in following ratio
Wheat bran: 80% manioc waste: 10%
Dregs of beans: 10% material: water 1: 0.9
2.3 material processing method
In dregs of beans and water added jar, soaked 30 minutes-1 hour, stir and want uniformity, add wheat bran then and manioc waste stirs, generally mixing time is 40 minutes, closes charging aperture after stirring
2.4 material boiling
2.4.1 chuck preheating
Open the chuck outlet valve, open the jacket steam valve, feed small amount of steam, the water in the emptying chuck
Closed clip cover outlet valve is driven big jacket steam valve, pre-thermal jacket to 90 ℃
2.4.2 material boiling and sterilization
Close the chuck steam valve, open the tank body steam valve, feed steam
Pressure cooking treats that generally pressure is that 0.15MP, temperature are about 121 ℃, and boiling 30 minutes picks up counting
2.5 pipeline sterilization
Comprise that inoculation pipeline and nutrient solution add the pipeline sterilization
Opening conduits steam inlet valve cuts out tank body pipeline intake valve, and condensed water in the opening conduits air outlet valve, emptying pipe is treated to transfer big intake valve after steam is evenly discharged, and turns air outlet valve down, sterilization 30min
2.6 inoculation
After the material boiling is good, open compressed air, open chuck recirculated water, be cooled to room temperature rapidly
Open the liquid tank bleeder valve, utilize pressure differential that liquid seeds is sucked the solid jar, inoculum concentration is 3%
2.7 nutrient solution adds
2.7.1 nutrient solution preparation
Press following formulated nutrient solution:
Ammonium sulfate 3g potassium dihydrogen phosphate 3g
Epsom salt 3g calcium chloride 1.5g
Copper sulphate 0.5g sodium chloride 0.5g
Glucose 20g vitamin 0.5g
2.7.2 nutrient solution sterilization
Above-mentioned nutrient solution is configured the 115 ℃ of sterilizations of replenisher tank 10-15 minute of packing into by solid material 1%
After treating its cooling, open the replenisher tank bleeder valve, utilize pressure differential that nutrient solution is sucked the solid jar
2.8 fermenting and producing
Open chuck draining valve and steam valve, steam flow in the control chuck remains on about 30 ℃ the tank body temperature
Open paddle, 20 rev/mins of control mixing speeds (intermittently stirring)
Open the logical people's filtrated air of compressed air, air flow control is at 10-15m
3/ h
About fermenting and producing 30 hours
3 No. 3 seed solid tank fermentation technology flow processs
3.1 the cleaning of fermentation tank tank body
Fully wash tank skin or in tank body, fill with water with running water, open paddle, till cleaning up.Clean up the water in the drain tank of back
3.2 the interpolation of solid material
The solid material and the water that crush are added in the jar in following ratio
Wheat bran: 80% manioc waste: 10%
Dregs of beans: 10% material: water 1: 0.8
3.3 material processing method
In dregs of beans and water added jar, soaked 30 minutes-1 hour, stir and want uniformity, add wheat bran then and manioc waste stirs, generally mixing time is 40 minutes, closes charging aperture after stirring
3.4 material boiling
3.4.1 chuck preheating
Open the chuck outlet valve, open the jacket steam valve, feed small amount of steam, the water in the emptying chuck
Closed clip cover outlet valve is driven big jacket steam valve, pre-thermal jacket to 90 ℃
3.4.2 material boiling and sterilization
Close the chuck steam valve, open the tank body steam valve, feed steam
Pressure cooking treats that generally pressure is that 0.15MP, temperature are about 121 ℃, and boiling 30 minutes picks up counting
3.5 pipeline sterilization
Comprise that inoculation pipeline and nutrient solution add the pipeline sterilization
Opening conduits steam inlet valve cuts out tank body pipeline intake valve, and condensed water in the opening conduits air outlet valve, emptying pipe is treated to transfer big intake valve after steam is evenly discharged, and turns air outlet valve down, sterilization 30min
3.6 inoculation
After the material boiling is good, open compressed air, open chuck recirculated water, be cooled to room temperature rapidly
Open the liquid tank bleeder valve, utilize pressure differential that liquid seeds is sucked the solid jar, inoculum concentration is 3%
3.7 nutrient solution adds
3.7.1 nutrient solution preparation
Press following formulated nutrient solution:
Ammonium sulfate 3g potassium dihydrogen phosphate 3g
Epsom salt 3g calcium chloride 1.5g
Copper sulphate 0.5g sodium chloride 0.5g
Glucose 20g vitamin 0.5g
3.7.2 nutrient solution sterilization
Above-mentioned nutrient solution is configured the 115 ℃ of sterilizations of replenisher tank 10--15 minute of packing into by solid dry material 2%
After treating its cooling, open the replenisher tank bleeder valve, utilize pressure differential that nutrient solution is sucked the solid jar
3.8 fermenting and producing
Open chuck draining valve and steam valve, steam flow in the control chuck remains on about 30 ℃ the tank body temperature
Open paddle, 20 rev/mins of control mixing speeds (intermittently stirring)
Open the logical people's filtrated air of compressed air, air flow control is at 10-15m
3/ h
About fermenting and producing 30 hours
Plant bent preparation
1 No. 1 bent growth technique flow processs of seed kind
Cultivate 1.1 pile up
The song material is piled up in the zone of regulation with mound shape, and this moment, the product temperature should be 30-31 ℃, kept 29-31 ℃ of room temperature every temperature of 2h record, reached 35-36 ℃ through product temperature about 18h
1.2 shakeout the wet straw mat of lid
Every temperature of 1h note
Continue the about 12h of heat insulating culture, the product temperature reaches again about 36 ℃.
Treat that bent material surface is little white, and shakeout bent material when beginning to lump, make it loose.Shakeouing thickness is the every then heap lid of 50-80mm sterilization straw mat, preserve moisture (humidity is 100%)
1.3 turn over song for the second time
Stand Qu Houji continuation of insurance temperature cultivation 24h, bent temperature rises to 36 ℃ again, and bent material covers with white hypha, and caking is good, can carry out turning over song the second time, or according to circumstances draw song, with the bamboo bar song material is divided into the 5cm fragment
Still build wet straw mat after turning over song, temperature of every 1h record; Too high should the increasing according to actual conditions of temperature turned over bent number of times
Reducing room temperature is 25-28 ℃
1.4 sprinkle water, be incubated, preserve moisture
After drawing song, it is 100% that cold water maintenance indoor humidity should be often spilt on ground, reduces room temperature and makes the product temperature remain on 34-36 ℃, and temperature is too high should turn over song according to actual conditions
1.5 burring seat
From about 36h straw mat being removed behind the lid straw mat, bent temperature rises and relaxes, and stops to sprinkle water earthward, and the fan hydrofuge that begins to rehearse keeps about 30 ℃ of room temperatures, and 35-36 ℃ of product temperature is bent ripe to planting
Enter the room to kind of a bent maturation from 30 hours kind songs of solid jar fermentation, whole incubation time amounts to 60h.Plant in the bent preparation process and should write down a substandard products temperature, room temperature and operational circumstances every 1-2h
2 No. 2 bent growth technique flow processs of seed kind
Cultivate 2.1 pile up
The song material is piled up in the zone of regulation with mound shape, and this moment, the product temperature should be 30-31 ℃, kept 29-31 ℃ of room temperature every temperature of 2h record, reached 35-36 ℃ through product temperature about 8h
2.2 shakeout the wet straw mat of lid
Every temperature of 1h note
Continue the about 6h of heat insulating culture, the product temperature reaches again about 36 ℃.
Treat that bent material surface is little white, and shakeout bent material when beginning to lump, make it loose.Shakeouing thickness is the every then heap lid of 50-80mm sterilization straw mat, preserve moisture (humidity is 100%)
2.3 turn over song for the second time
Stand Qu Houji continuation of insurance temperature is trained 10h, and bent temperature rises to 36 ℃ again, and bent material covers with white hypha, and caking is good, can carry out turning over song the second time, or according to circumstances draw song, with the bamboo bar song material is divided into the 5cm fragment
Still build wet straw mat after turning over song, temperature of every 1h record; Too high should the increasing according to actual conditions of temperature turned over bent number of times
Reducing room temperature is 25-28 ℃
2.4 sprinkle water, be incubated, preserve moisture
After drawing song, ground should often be spilt cold water and be kept indoor humidity, reduces room temperature and makes the product temperature remain on 34-36 ℃, and temperature is too high should turn over song according to actual conditions, guarantees that humidity is 100%
2.5 burring seat
From about 16h straw mat being removed behind the lid straw mat, bent temperature rises and relaxes, and stops to sprinkle water earthward, and the fan hydrofuge that begins to rehearse keeps about 30 ℃ of room temperatures, and 35-36 ℃ of product temperature is bent ripe to planting
Enter the room to kind of a bent maturation from 30 hours kind songs of solid jar fermentation, whole incubation time amounts to 30h.Plant in the bent preparation process and should write down a substandard products temperature, room temperature and operational circumstances every 1-2h
3 No. 3 bent growth technique flow processs of seed kind
Cultivate 3.1 pile up
The song material is piled up in the zone of regulation with mound shape, and this moment, the product temperature should be 30-31 ℃, kept 29-31 ℃ of room temperature every temperature of 2h record, reached 30-32 ℃ through product temperature about 8h
3.2 shakeout the wet straw mat of lid
Every temperature of 1h note
Continue the about 12h of heat insulating culture, the product temperature reaches again about 32 ℃
Treat that bent material surface is little white, and shakeout bent material when beginning to lump, make it loose.Shakeouing thickness is the every then heap lid of 50-80mm sterilization straw mat, preserve moisture (humidity is 100%)
3.3 turn over song for the second time
Stand Qu Houji continuation of insurance temperature cultivation 16h, bent temperature rises to 32 ℃ again, and bent material covers with white hypha, and caking is good, can carry out turning over song the second time, or according to circumstances draw song, with the bamboo bar song material is divided into the 5cm fragment
Still build wet straw mat after turning over song, temperature of every 1h record; Too high should the increasing according to actual conditions of temperature turned over bent number of times
Reducing room temperature is 25-28 ℃
3.4 sprinkle water, be incubated, preserve moisture
After drawing song, ground should often be spilt cold water and be kept indoor humidity, reduces room temperature and makes the product temperature remain on 30-32 ℃, and temperature is too high should turn over song according to actual conditions, and humidity is 100%
3.5 burring seat
From about 28h straw mat being removed behind the lid straw mat, bent temperature rises and relaxes, and stops to sprinkle water earthward, and the fan hydrofuge that begins to rehearse keeps about 30 ℃ of room temperatures, and 30--32 ℃ of product temperature is bent ripe to planting
Enter the room to kind of a bent maturation from 30 hours kind songs of solid jar fermentation, whole incubation time amounts to 42h.Plant in the bent preparation process and should write down a substandard products temperature, room temperature and operational circumstances every 1-2h
Plant the points for attention in the bent growth course
1. fermenting house sterilization
Should open uviol lamp sterilization 10-20 minute before every batch of production, and regularly the room be fumigated
2. the sterilization of straw mat
Straw mat is put abundant poach in the adjunce copper, reach the purpose of sterilization
3. material enters
The utensil of dress material should guarantee aseptic, and the alcohol of application 75% fully cleans before sending at every turn; Should above materials vehicle, add a cover thin film in the process of haul, prevent that airborne assorted cingula from going into
4. turn over the throwing utensil
Turn over the throwing utensil and should guarantee the special use of special room, prevent cross-infection; And regularly carry out disinfection
5. personnel's turnover
Producers pass in and out fermenting house should wear work clothes, footware
Should on the haystack of spray disinfectant, stamp one's foot for several times before each turnover, and wash one's hands with hand cleanser; Guaranteeing does not have cross-infection
Mixed fungus fermentation
1 solid mixed fungus fermentation technological process
1.1 material is got:
Get the solid fermentation raw material by following prescription:
Manioc waste (wetting) 250kg dregs of beans: 5.0kg
Ammonium chloride: 1.5kg urea: 2.0kg
Potassium dihydrogen phosphate: 1.2kg
1.2 material is handled:
Manioc waste moisture guarantees between 50-60%, treats to determine to add amount of moisture after given data is given in the laboratory
Produce the previous day, to the wet manioc waste sterilization of off-the-shelf moisture between 50-60%
With dregs of beans, ammonium chloride, urea, potassium dihydrogen phosphate, admix manioc waste uniformly after adding a spot of water-soluble separating after getting by prescription
1.3 plant bent interpolation
No. 1, No. 3 kind songs of interpolation are evenly admixed bent material earlier, shakeout piling height and are: 50-80cm
Treat that its growth adds No. 2 kind songs after 2 days, it is the same to shakeout piling height after mixing thoroughly
Add No. 4 seeds after bent about 3 days from adding No. 1, No. 3 kinds, mix the back accumulated growth thoroughly
Add the back that finishes from seed and finished fermentation in about 2 days
After fermentation is finished, finished product is mound shape accumulation can transfers subsequent processing in 24 hours
Points for attention in 2 sweats
2.1 temperature raises comparatively fast in the sweat, should write down temperature one time every 1h, maintains the temperature between 35-45 ℃
2.2 the stirring number of times is difficult for too much treating that its caking well can strengthen the stirring number of times in right amount
2.3 too high because of mixing the bent inhomogeneous local temperature that causes, stirring according to circumstances, and carry out relative recording
2.4 carry out fermentation change color record according to kind of a bent growing state
2.5 turning over bent instrument should regularly sterilize, sterilize
2.6 producers are answering work clothes, footware
Description of drawings: accompanying drawing is the process chart of patent of the present invention.
Claims (1)
1. manioc waste feed resource technology is utilized to filter out from bacterium and fungi and can be utilized cellulosic bacterial strain in the degraded manioc waste; Carry out mutagenesis through multiple modes such as physics and chemistry, filter out the high bacterial strain of cellulase and mycoprotein output as producing bacterial classification. by to each strain fermentation culture medium and Optimizing Conditions of Fermentation with research and solve the pollution problem of manioc waste, making its fermenting and producing is the high protein feed that animal absorbs easily.
1.1 number, No. 2, No. 3, No. 4 breeding strains and method for preserving.
2.1 number, fermentation condition such as No. 2, No. 3, No. 4 bacterial classification liquid fermentative medium formulas, inoculum concentration, inoculation time, fermentation temperature, fermentation time, pH value, throughput.
3.1 number, each medium component addition, inoculum concentration, nutrient composition and addition in No. 2, No. 3, No. 4 bacterial classification kind koji fermentation culture mediums, and associated fermentation condition.
Material formula in the mixed fungus fermentation process, plant bent addition and add sequential scheduling associated production technology.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010107505XA CN102144713A (en) | 2010-02-09 | 2010-02-09 | Cassava residue feedstuff recycling technology |
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| CN201010107505XA CN102144713A (en) | 2010-02-09 | 2010-02-09 | Cassava residue feedstuff recycling technology |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102599344A (en) * | 2012-04-18 | 2012-07-25 | 重庆市中药研究院 | Cassava residue fermented feed and preparation method thereof |
| CN102696875A (en) * | 2012-06-26 | 2012-10-03 | 广西九通王环保生物工程有限公司 | Method for preparing biological feed by taking manioc residues as raw materials |
-
2010
- 2010-02-09 CN CN201010107505XA patent/CN102144713A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102599344A (en) * | 2012-04-18 | 2012-07-25 | 重庆市中药研究院 | Cassava residue fermented feed and preparation method thereof |
| CN102696875A (en) * | 2012-06-26 | 2012-10-03 | 广西九通王环保生物工程有限公司 | Method for preparing biological feed by taking manioc residues as raw materials |
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Application publication date: 20110810 |