CN103749943A - Method for producing biological feed by fermenting byproducts from deep processing of corns - Google Patents
Method for producing biological feed by fermenting byproducts from deep processing of corns Download PDFInfo
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Abstract
The invention relates to a method for producing biological feed by fermenting byproducts from deep processing of corns, which belongs to the field of fermentation engineering. The method comprises the steps of respectively culturing strains of bacillus subtilis, lactobacillus and microzyme, mixing the bacillus subtilis strain, the lactobacillus strain and the microzyme strain according to the ratio of 1:1:3 to obtain fermenting strain liquid, mixing corn bran, corn pulp, sugar slag and corn germ dregs according to a given ratio to obtain a fermenting culture medium, adding the fermenting strain liquid into the fermenting culture medium, uniformly mixing the fermenting strain liquid with the fermenting culture medium to obtain a mixture, filling the mixture into a vacuum bag, sealing, compacting, culturing and fermenting the mixture at the temperature of 37 DEG C for 60h, and taking out the mixture. By adopting the method, the disease-resistant immunity of animals can be improved, the micro-ecological balance of animal intestines can be improved, and the animal product quality can be improved; in addition, the animal immunity can be enhanced after different living probiotics produced in the fermentation process enter the animal body, the utilization rate of the feed raw material can be increased, and the breeding cost can be reduced.
Description
Technical field
The invention belongs to field of fermentation engineering, be specifically related to utilize the method for corn deep processing byproduct fermentation to produce biological feed.
Background technology
Corn deep processing byproduct has corn bran, corn germ cake, sugared slag, corn steep liquor and fresh water (FW) etc., in the cud of ruminant, by the spontaneous probio of cud, resolve into carbohydrate and little peptide, through bacterium, synthesize mycoprotein or directly by animal, absorbed again, but the cud probio number of ruminant is limited, and digestibility is also lower.
Summary of the invention
The invention provides a kind of method of utilizing corn deep processing byproduct fermentation to produce biological feed, limited to solve the cud probio number of ruminant, to the lower problem of corn deep processing byproduct feed digestibility.
The technical scheme that the present invention takes comprises the following steps:
(1) bacillus subtilis, lactic acid bacteria, saccharomycete are cultivated respectively to bacterial classification, in the ratio of 1:1:3, mix and make zymocyte liquid;
Described bacillus subtilis bacterium culture medium: glucose 20g, peptone 15g, sodium chloride 5g, beef extract 0.5g, agar 20g;
Described lactic acid bacteria culture medium: yeast extract 7.5g, glucose 10g, peptone 7.5g, dipotassium hydrogen phosphate 2g, Pan's tomato juice 100ml, Tween800.5ml;
Described microzyme culture medium: potato 20g, glucose 2g, agar 2g;
(2) by corn bran, corn steep liquor, sugared slag, the plumule dregs of rice with (1～3): the mass ratio of (1～2): 1:1 mixes, for the preparation of fermentation medium;
(3) by mass percent, be 1%～2% inoculation fermentation bacterium liquid, add in fermentation medium, mix;
(4) mixture step (3) being obtained is inserted vacuum bag sealing compacting, and cultivation and fermentation under 37 ℃ of conditions, finishes fermentation in 60h, takes out.
The present invention carries out fermentation process by microorganism to corn deep processing byproduct, utilize the metabolic activity of the microorganisms self such as lactic acid bacteria, saccharomycete, bacillus, the fiber that indigestibility is utilized, high molecular weight protein etc. decompose or transform, produce more easily and digested, absorb by livestock and poultry, and the fermentative feedstuff of microbe of nonhazardous effect.Microorganism is in feed fermentation process, growth metabolism can produce various enzymes, and this macromolecular substances by complexity such as the starch in feedstuff, protein, cellulose, pectin is converted into polysaccharide, monose, little peptide, amino acid etc. and is more conducive to the little molecular nutrition material that animal absorbs; Meanwhile, microorganism can produce various active material in metabolic process, as organic acid, polysaccharide, antibacterial substance, active peptide etc., can improve the anti pathologic immunity ability of animal body, improves animal intestinal microecological balance, improves animal product quality; In addition, the various probiotics viable bacterias that produce in sweat, after entering in animal body, strengthen the immunity of animal, the utilization rate that improves feedstuff, reduction feeding cost.
By the checking of test, fermentation process provided by the present invention can effectively improve the Vitro Digestibility of product.
The specific embodiment
Embodiment 1
(1) bacillus subtilis, lactic acid bacteria, saccharomycete are cultivated respectively to bacterial classification, in the ratio of 1:1:3, mix and make zymocyte liquid;
Described bacillus subtilis bacterium culture medium: glucose 20g, peptone 15g, sodium chloride 5g, beef extract 0.5g, agar 20g;
Described lactic acid bacteria culture medium: yeast extract 7.5g, glucose 10g, peptone 7.5g, dipotassium hydrogen phosphate 2g, Pan's tomato juice 100ml, Tween800.5ml;
Described microzyme culture medium: potato 20g, glucose 2g, agar 2g;
(2) corn bran, corn steep liquor, sugared slag, the plumule dregs of rice are mixed with the mass ratio of 1:1:1:1, for the preparation of fermentation medium;
(3) by mass percent, be 1%～2% inoculation fermentation bacterium liquid, add in fermentation medium, mix;
(4) mixture step (3) being obtained is inserted vacuum bag sealing compacting, and cultivation and fermentation under 37 ℃ of conditions, finishes fermentation in 60h, takes out;
After taking-up, carry out sample detection; The pH value that sampling detects sample is 5.26 and Vitro Digestibility 73.0%.
Embodiment 2
(1) bacillus subtilis, lactic acid bacteria, saccharomycete are cultivated respectively to bacterial classification, in the ratio of 1:1:3, mix and make zymocyte liquid;
Described bacillus subtilis bacterium culture medium: glucose 20g, peptone 15g, sodium chloride 5g, beef extract 0.5g, agar 20g;
Described lactic acid bacteria culture medium: yeast extract 7.5g, glucose 10g, peptone 7.5g, dipotassium hydrogen phosphate 2g, Pan's tomato juice 100ml, Tween800.5ml;
Described microzyme culture medium: potato 20g, glucose 2g, agar 2g;
(2) corn bran, corn steep liquor, sugared slag, the plumule dregs of rice are mixed with the mass ratio of 2:1.5:1:1, for the preparation of fermentation medium;
(3) by mass percent, be 1%～2% inoculation fermentation bacterium liquid, add in fermentation medium, mix;
(4) mixture step (3) being obtained is inserted vacuum bag sealing compacting, and cultivation and fermentation under 37 ℃ of conditions, finishes fermentation in 60h, takes out;
The pH value that sampling detects sample is 5.26 and Vitro Digestibility 68.4%.
Embodiment 3:
(1) bacillus subtilis, lactic acid bacteria, saccharomycete are cultivated respectively to bacterial classification, in the ratio of 1:1:3, mix and make zymocyte liquid;
Described bacillus subtilis bacterium culture medium: glucose 20g, peptone 15g, sodium chloride 5g, beef extract 0.5g, agar 20g;
Described lactic acid bacteria culture medium: yeast extract 7.5g, glucose 10g, peptone 7.5g, dipotassium hydrogen phosphate 2g, Pan's tomato juice 100ml, Tween800.5ml;
Described microzyme culture medium: potato 20g, glucose 2g, agar 2g;
(2) corn bran, corn steep liquor, sugared slag, the plumule dregs of rice are mixed with the mass ratio of 3:2:1:1, for the preparation of fermentation medium;
(3) by mass percent, be 1%～2% inoculation fermentation bacterium liquid, add in fermentation medium, mix;
(4) mixture step (3) being obtained is inserted vacuum bag sealing compacting, and cultivation and fermentation under 37 ℃ of conditions, finishes fermentation in 60h, takes out;
The pH value that sampling detects sample is 5.12 and Vitro Digestibility 74.0%.
Claims (1)
1. a method of utilizing corn deep processing byproduct fermentation to produce biological feed, is characterized in that comprising the following steps:
(1) bacillus subtilis, lactic acid bacteria, saccharomycete are cultivated respectively to bacterial classification, in the ratio of 1:1:3, mix and make zymocyte liquid;
Described bacillus subtilis bacterium culture medium: glucose 20g, peptone 15g, sodium chloride 5g, beef extract 0.5g, agar 20g;
Described lactic acid bacteria culture medium: yeast extract 7.5g, glucose 10g, peptone 7.5g, dipotassium hydrogen phosphate 2g, Pan's tomato juice 100ml, Tween800.5ml;
Described microzyme culture medium: potato 20g, glucose 2g, agar 2g;
(2) by corn bran, corn steep liquor, sugared slag, the plumule dregs of rice with (1～3): the mass ratio of (1～2): 1:1 mixes, for the preparation of fermentation medium;
(3) by mass percent, be 1%～2% inoculation fermentation bacterium liquid, add in fermentation medium, mix;
(4) mixture step (3) being obtained is inserted vacuum bag sealing compacting, and cultivation and fermentation under 37 ℃ of conditions, finishes fermentation in 60h, takes out.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104621343A (en) * | 2014-12-25 | 2015-05-20 | 山东西王糖业有限公司 | Production method of liquid feed |
| CN104642729A (en) * | 2015-02-09 | 2015-05-27 | 山东西王糖业有限公司 | Method for producing protein feed by using corn deep-processing by-products as raw material |
| CN108077559A (en) * | 2017-11-28 | 2018-05-29 | 吉林中粮生化有限公司 | A kind of fermentative feedstuff of microbe and preparation method thereof |
| CN108850548A (en) * | 2018-06-22 | 2018-11-23 | 河南飞天农业开发股份有限公司 | A kind of production technology for maize peel of whitewashing |
| CN109393145A (en) * | 2018-12-21 | 2019-03-01 | 北安象屿金谷生化科技有限公司 | The method for preparing biological fermentation feed using the intermediate product of wet production cornstarch |
| CN109793097A (en) * | 2018-12-26 | 2019-05-24 | 河南巨龙生物工程股份有限公司 | A kind of fermentation process using sugaring tailing and tryptophan mother liquor production biological feedstuff |
| CN111733114A (en) * | 2020-08-04 | 2020-10-02 | 山东理工大学 | Method for preparing microecological bactericide by using corn steep liquor as raw material and utilizing symbiotic fermentation technology |
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| CN101449741A (en) * | 2007-11-30 | 2009-06-10 | 北京嘉博文生物科技有限公司 | Multifunctional biology corn feedstuff |
| CN102178033A (en) * | 2011-04-22 | 2011-09-14 | 河南宏翔生物科技有限公司 | Combined bacteria fermented bran high protein predigesting feed and preparation method |
| CN103070292A (en) * | 2013-02-20 | 2013-05-01 | 唐山师范学院 | Method for producing microecological feed additives for livestocks by using agricultural and sideline products |
| CN103355474A (en) * | 2013-07-19 | 2013-10-23 | 上海创博生态工程有限公司 | Microbial preparation for fermentation production of fodder |
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Patent Citations (4)
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| CN101449741A (en) * | 2007-11-30 | 2009-06-10 | 北京嘉博文生物科技有限公司 | Multifunctional biology corn feedstuff |
| CN102178033A (en) * | 2011-04-22 | 2011-09-14 | 河南宏翔生物科技有限公司 | Combined bacteria fermented bran high protein predigesting feed and preparation method |
| CN103070292A (en) * | 2013-02-20 | 2013-05-01 | 唐山师范学院 | Method for producing microecological feed additives for livestocks by using agricultural and sideline products |
| CN103355474A (en) * | 2013-07-19 | 2013-10-23 | 上海创博生态工程有限公司 | Microbial preparation for fermentation production of fodder |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104621343A (en) * | 2014-12-25 | 2015-05-20 | 山东西王糖业有限公司 | Production method of liquid feed |
| CN104642729A (en) * | 2015-02-09 | 2015-05-27 | 山东西王糖业有限公司 | Method for producing protein feed by using corn deep-processing by-products as raw material |
| CN108077559A (en) * | 2017-11-28 | 2018-05-29 | 吉林中粮生化有限公司 | A kind of fermentative feedstuff of microbe and preparation method thereof |
| CN108850548A (en) * | 2018-06-22 | 2018-11-23 | 河南飞天农业开发股份有限公司 | A kind of production technology for maize peel of whitewashing |
| CN109393145A (en) * | 2018-12-21 | 2019-03-01 | 北安象屿金谷生化科技有限公司 | The method for preparing biological fermentation feed using the intermediate product of wet production cornstarch |
| CN109793097A (en) * | 2018-12-26 | 2019-05-24 | 河南巨龙生物工程股份有限公司 | A kind of fermentation process using sugaring tailing and tryptophan mother liquor production biological feedstuff |
| CN111733114A (en) * | 2020-08-04 | 2020-10-02 | 山东理工大学 | Method for preparing microecological bactericide by using corn steep liquor as raw material and utilizing symbiotic fermentation technology |
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Application publication date: 20140430 |