CN103355474A - Microbial preparation for fermentation production of fodder - Google Patents

Microbial preparation for fermentation production of fodder Download PDF

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CN103355474A
CN103355474A CN2013103075382A CN201310307538A CN103355474A CN 103355474 A CN103355474 A CN 103355474A CN 2013103075382 A CN2013103075382 A CN 2013103075382A CN 201310307538 A CN201310307538 A CN 201310307538A CN 103355474 A CN103355474 A CN 103355474A
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bacillus
weight
microorganism formulation
weight portion
nutriment
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江瀚
张小峰
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SHANGHAI CHUANGBO ECOLOGICAL ENGINEERING Co Ltd
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SHANGHAI CHUANGBO ECOLOGICAL ENGINEERING Co Ltd
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Abstract

The invention provides a microbial preparation for fermentation production of a fodder. The microbial preparation comprises the components, in parts by weight: 2-20 parts by weight of yeast, 15-60 parts by weight of bacillus, and 30-60 parts by weight of lactic acid bacteria. The microbial preparation provided by the invention acts on a fodder raw material and performs solid fermentation to prepare a microbial fermented fodder which can improve the quality of mutton, the microbial fermented fodder is more easily absorbed and utilized by stomach, so that ultimately the growth rate of meat sheep can be accelerated, the sheep can be slaughtered early, the water loss rate of the mutton is obviously reduced, and the intramuscular fat content and the intramuscular amino acid content are significantly increased.

Description

A kind of microorganism formulation of fermenting and producing feed
Technical field
The present invention relates to a kind of microorganism formulation, the fermentative feedstuff of microbe that relates in particular to a kind of microorganism formulation of fermenting and producing feed and adopt this microorganism formulation fermentation to prepare.
Background technology
Along with domestic economic development and growth in the living standard, the price of mutton is also soaring year by year in recent years, rises to 60 yuan of present per kilograms by about 25 yuan of the per kilograms before 3 years and does not wait to 90 yuan.The increase of economic benefit impels mutton sheep plant to occur like the mushrooms after rain in the whole nation.Owing in breeding process, add growth-promoting class antibiotic etc., cause quality of mutton to descend, taste is not bright.
Microorganism probiotic develops comparatively rapid advancing year.Various microbe additives are widely used in having formed comparatively considerable ecological benefits, Social benefit and economic benefit in the cultivation, are also approved by law enforcement agency of government, and have strengthened the dynamics of promoting.
For example CN_103053791A discloses a kind of fermentative feedstuff of microbe, by following component by weight mark form than mixing fermentation: 351 parts of peanut hull meals, 32 parts in inferior powder, 20 parts in wheat bran, husky 54 parts of worm, 500 parts of brewex's grains, 20 parts of premixes, 23 parts of bacterium liquid; Described premix by following component by weight the mark ratio form: 452 parts of stone flours, 330 parts of zeolite powders, 24 parts of compound vitamins, 50 parts of calcium monohydrogen phosphates, 50 parts of maize peels of whitewashing, 90 parts of salt, 2 parts of flavouring agents, 2 parts of sweeteners; Described bacterium liquid is the mixed bacteria liquid of Pichia pastoris Hzd-pj-003 bacterium liquid, bacillus subtilis Hzd-bs-001 bacterium liquid and Lactobacillus casei Hzd-lc-002 bacterium liquid.This fermentative feedstuff of microbe utilizes cheap crops leftovers by microbial solid fermentation, turns waste into wealth, and forms the full-nutrients biological fermentation section grain environment-protecting feed that a new generation integrates nutrition, diseases prevention, material-saving, ecological quadruple effect.
And for example CN1103039699A discloses a kind of preparation method of mixed fermentation feed, it is characterized in that: by lactobacillus paraceasi, bacillus subtilis, saccharomyces cerevisiae difference liquid fermentation and culture, carry out the solid fermentation cultivation after the by volume 1:1:1 mixing of zymotic fluid and form, it is directly feeding that product need not drying.The preparation method of this fermented feed has reduced antibiotic use, resulting fermented feed contains the nutriments such as a large amount of mycoproteins, abundant amino acid and vitamin, is conducive to promote the gut flora balance, improves fowl poultry immunity of organisms, improve production performance, reduce feeding cost.
But how existing fermentative feedstuff of microbe improves immunity of organisms by strengthening the resistance against diseases of animal, promotes the animal health growth, the use of minimizing growth-promoting class antibiotic etc., thus the decline of reduction animal meat can not fundamentally promote meat.
Summary of the invention
The object of the invention is to overcome above-mentioned deficiency, a kind of microorganism formulation of fermenting and producing feed is provided, the preparation fermentative feedstuff of microbe, absorbed by stomach and utilize easilier, so that the speed of growth of mutton sheep is accelerated, the percentage of water loss of mutton obviously descends, and significantly promotes meat.
First aspect of the present invention provides a kind of microorganism formulation of fermenting and producing feed, according to the parts by weight meter, comprising:
Saccharomycete 2-20 weight portion (preferred 3-15 weight portion, more preferably 5-10 weight portion, more preferably 6-8 weight portion);
Bacillus 15-60 weight portion (preferred 20-55 weight portion, more preferably 22-50 weight portion, more preferably 25-45 weight portion, more preferably 30-40 weight portion);
Lactic acid bacteria 30-60 weight portion (preferred 35-55 weight portion, more preferably 40-50 weight portion, more preferably 42-48 weight portion).
Wherein, described bacillus is the bacillus that is used for being used for Production of Livestock and Poultry both at home and abroad, can be selected from bacillus subtilis, bacillus coagulans, slow bacillus, bacillus licheniformis, bacillus pumilus, bacillus cereus, Bacillus circulans, bacillus megaterium, bacillus firmus, Japan bacillus, bafillus natto, lactobacillus and the clostridium butyricum one or more.
Preferably, described bacillus comprises bacillus licheniformis and bacillus subtilis.
Further preferably, comprise bacillus licheniformis 5-25 weight portion (preferred 8-22 weight portion, more preferably 10-20 weight portion, more preferably 13-17 weight portion) in the described microorganism formulation.
Further preferably, also comprise bacillus subtilis 10-35 weight portion (preferred 12-30 weight portion, more preferably 15-25 weight portion, more preferably 18-22 weight portion) in the described microorganism formulation.
Preferably, viable count is preferably 〉=3,000,000,000/g in the described microorganism formulation.
Wherein, described microorganism formulation can be liquid preparation or solid pharmaceutical preparation, is preferably solid pharmaceutical preparation, more preferably particle and/or powder, average grain diameter is preferably the 10-100 order, more preferably the 20-80 order, more preferably the 30-70 order most preferably is the 40-60 order, such as 50 orders.
Described microorganism formulation is preferably solid pharmaceutical preparation.
Wherein, microorganism formulation can also comprise carrier, and described carrier can be liquid-carrier, such as water; Also can be solid carrier, such as any one or a few the mixture in zeolite, calcium carbonate, kaolin (comprising calcined kaolin), the bentonite.
Wherein, described microorganism formulation can also comprise nutriment, and described nutriment can be any one or a few the mixture that is selected from starch (being preferably cornstarch), molasses, trehalose, sucrose, maltose, isomaltose, wood sugar, fructose, stachyose, the gossypose.
Preferably, take the gross weight of carrier (being preferably solid carrier) and nutriment as benchmark, the percentage by weight of carrier is 80-90w%, and the percentage by weight of nutriment is 10-20wt%.
Preferably, described carrier is the combination of zeolite powder and calcium carbonate, and described nutriment is the combination of cornstarch and molasses; Wherein, the zeolite powder percentage by weight is 65-75wt%, and weight of calcium carbonate percentage is 10-20wt%; The percentage by weight of cornstarch is 5-15wt%, and the percentage by weight of molasses is 1-10wt%.
Microorganism formulation of the present invention is composited by saccharomycete, lactic acid bacteria, bacillus licheniformis (bacillus licheniformis and bacillus subtilis etc.) etc., acting on feedstuff carries out solid fermentation and makes the fermentative feedstuff of microbe that can improve quality of mutton, in the mutton sheep cultivation, have following effect: a and fully transform decomposition nutrition matter, large molecular raw material is changed into the easier small-molecule substance that is absorbed by stomach; The quantity of beneficial microbe thalline in the b Effective Raise raw material, thus harmful microbe quantity reduced; The c palatability is better, and feed intake and efficiency of feed utilization that can the Effective Raise mutton sheep reduce feedstuff-meat ratio.
The invention has the advantages that: a microorganism formulation provided by the invention by with feedstuff fermentation absorbed by stomach and utilize so that the fermentative feedstuff of microbe that fermentation obtains is easier, the final so that speed of growth quickening of mutton sheep, deliver for sale ahead of time; The percentage of water loss of b mutton obviously descends, and fat content, amino acid content significantly promote between flesh.
The specific embodiment
The invention will be further described below in conjunction with specific embodiment, to understand better the present invention.
Embodiment 1
The first step: preparation bacterium culture medium
It is 6.5 complex medium that beef extract 1.5wt%, peptone 1wt%, potassium dihydrogen phosphate 0.01wt%, magnesium sulfate 0.03wt%, vitamin B complex 0.75wt% are dissolved into pH value with warm water.
Second step: preparation composite bacteria liquid
Microorganism formulation is inoculated in the complex medium that the first step makes according to the part by weight of 7:100, microorganism formulation is comprised of saccharomycete 5 weight portions, bacillus licheniformis 10 weight portions, lactic acid bacteria 40 weight portions, bacillus subtilis 15 weight portions, under 30 ℃ of temperature, cultivated 100 hours, obtain composite bacteria liquid.
The 3rd step: make the fermentative feedstuff of microbe finished product
Bacterium liquid 3 weight portions, corn flour 30 weight portions, molasses 3 weight portions, corn stalk powder 40 weight portions, concentrate feed 10 weight portions that second step is turned out, add water 25 weight portions and evenly mix after the barrelling sealing, in 30 ℃ environment, carried out anaerobic fermentation 10 days, fermentation ends when the solid pH value drops to 4.5 in the bucket obtains fermentative feedstuff of microbe.For feeding.
Embodiment 2
The first step: preparation bacterium culture medium
It is 6.5 complex medium that beef extract 1.7wt%, peptone 1.2wt%, potassium dihydrogen phosphate 0.02wt%, magnesium sulfate 0.04wt%, vitamin B complex 1wt% are dissolved into pH value with warm water.
Second step: preparation composite bacteria liquid
Microorganism formulation is inoculated in the complex medium that the first step makes according to the part by weight of 10:100, microorganism formulation is comprised of saccharomycete 7 weight portions, bacillus licheniformis 14 weight portions, lactic acid bacteria 42 weight portions, bacillus subtilis 18 weight portions, under 32 ℃ of temperature, cultivated 100 hours, obtain composite bacteria liquid.
The 3rd step: make the fermentative feedstuff of microbe finished product
Bacterium liquid 4 weight portions, corn flour 40 weight portions, molasses 4 weight portions, corn stalk powder 50 weight portions, concentrate feed 13 weight portions that second step is turned out, add water 30 weight portions and evenly mix after the barrelling sealing, in 30 ℃ environment, carried out anaerobic fermentation 12 days, fermentation ends when the solid pH value drops to 5.0 in the bucket obtains fermentative feedstuff of microbe.For feeding.
Embodiment 3
The first step: preparation bacterium culture medium
It is 6.5 complex medium that beef extract 1.8wt%, peptone 1.35wt%, potassium dihydrogen phosphate 0.03wt%, magnesium sulfate 0.05wt%, vitamin B complex 1.2wt% are dissolved into pH value with warm water.
Second step: preparation composite bacteria liquid
Microorganism formulation is inoculated in the complex medium that the first step makes according to the part by weight of 12:100, microorganism formulation is comprised of saccharomycete 9 weight portions, bacillus licheniformis 18 weight portions, lactic acid bacteria 46 weight portions, bacillus subtilis 22 weight portions, under 35 ℃ of temperature, cultivated 100 hours, obtain composite bacteria liquid.
The 3rd step: make the fermentative feedstuff of microbe finished product
Bacterium liquid 4.5 weight portions, corn flour 42 weight portions, molasses 4.5 weight portions, corn stalk powder 55 weight portions, concentrate feed 18 weight portions that second step is turned out, add water 35 weight portions and evenly mix after the barrelling sealing, in 30 ℃ environment, carried out anaerobic fermentation 15 days, fermentation ends when the solid pH value drops to 5.0 in the bucket obtains fermentative feedstuff of microbe.For feeding.
Embodiment 4
The first step: preparation bacterium culture medium
It is 6.5 complex medium that beef extract 2wt%, peptone 1.5wt%, potassium dihydrogen phosphate 0.03wt%, magnesium sulfate 0.05wt%, vitamin B complex 1.5wt% are dissolved into pH value with warm water.
Second step: preparation composite bacteria liquid
Microorganism formulation is inoculated in the complex medium that the first step makes according to the part by weight of 15:100, microorganism formulation is comprised of saccharomycete 10 weight portions, bacillus licheniformis 20 weight portions, lactic acid bacteria 50 weight portions, bacillus subtilis 25 weight portions, under 35 ℃ of temperature, cultivated 100 hours, obtain composite bacteria liquid.
The 3rd step: make the fermentative feedstuff of microbe finished product
Bacterium liquid 5 weight portions, corn flour 45 weight portions, molasses 5 weight portions, corn stalk powder 60 weight portions, concentrate feed 20 weight portions that second step is turned out, add water 30 weight portions and evenly mix after the barrelling sealing, in 30 ℃ environment, carried out anaerobic fermentation 15 days, fermentation ends when the solid pH value drops to 5.5 in the bucket obtains fermentative feedstuff of microbe.For feeding.
Impact on meat: after the feed comparison of fermentative feedstuff of microbe with normal diet that the mutton sheep of fattening stage is fed, detect fermentative feedstuff of microbe to the impact of mutton sheep different parts amino acid content, the result as shown in Table 1 and Table 2:
Amino acid index determining in the table 1 sheep muscle of back
Figure BDA00003540842600051
After testing, total amino acid content is 13.92g/100g in the test group muscle of back, total amino acid content is 12.89g/100g in the control group muscle of back, test group contrast group improves 7.99%, and wherein increase rate reaches being respectively more than 10%: methionine raising 14.29%, histidine raising 13.64%, glutamic acid 10.58%, isoleucine 10.34%.
Amino acid index determining in the table 2 sheep buttocks muscles
Figure BDA00003540842600062
After testing, total amino acid content is 14.64g/100g in the test group buttocks muscles, total amino acid content is 12.68g/100g in the control group buttocks muscles, test group contrast group improves 15.46%, and wherein increase rate reaches being respectively more than 10%: cystine raising 173.69%, methionine raising 47.83%, serine 22.41%, figured silk fabrics leucine 18.57%, arginine 15.63%, lysine 14.53%, aspartic acid 13.93%, tyrosine 12.12%, glutamic acid 10.33%; What average increment increased is cystine, and the unit increment is 0.33g/100g.
The present invention has utilized compound beneficial microorganism effect to decompose large molecular nutrition material in the raw material, and crude fibre has finally effectively improved the feed physicochemical property, makes that nutriment is easier in the fermentative feedstuff of microbe is absorbed by stomach.To be the high protein material act on the absorption of ruminant animal nutrition the saccharomycete of microorganism formulation to some extent, simultaneously through producing polysaccharide, amino acid, anti-oxidant class material have played useful improvement to the enteron aisle of ruminant the effect of being rich in the process of microorganism formulation fermentation, utilization, the conversion ratio of feed have been improved, reduced feedstuff-meat ratio, significantly reduced the percentage of water loss of mutton, obviously promote fat content between flesh, amino acid content, significantly improved meat.
More than specific embodiments of the invention are described in detail, but it is just as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that the present invention is carried out and substituting also all among category of the present invention.Therefore, not breaking away from impartial conversion and the modification of doing under the spirit and scope of the present invention, all should contain within the scope of the invention.

Claims (10)

1. the microorganism formulation of a fermenting and producing feed is characterized in that, according to the parts by weight meter, comprising: saccharomycete 2-20 weight portion, bacillus 15-60 weight portion, lactic acid bacteria 30-60 weight portion.
2. microorganism formulation according to claim 1, it is characterized in that described bacillus is selected from one or more in bacillus subtilis, bacillus coagulans, slow bacillus, bacillus licheniformis, bacillus pumilus, bacillus cereus, Bacillus circulans, bacillus megaterium, bacillus firmus, Japan bacillus, bafillus natto, lactobacillus and the clostridium butyricum.
3. microorganism formulation according to claim 1 is characterized in that, described bacillus comprises bacillus licheniformis and bacillus subtilis.
4. microorganism formulation according to claim 3 is characterized in that, comprises bacillus licheniformis 5-25 weight portion, bacillus subtilis 10-35 weight portion in the described microorganism formulation.
5. microorganism formulation according to claim 4 is characterized in that, described microorganism formulation comprises saccharomycete 5-10 weight portion, lactic acid bacteria 40-50 weight portion, bacillus licheniformis 10-20 weight portion, bacillus subtilis 15-25 weight portion.
6. microorganism formulation according to claim 1 is characterized in that, also comprises carrier and/or nutriment.
7. microorganism formulation according to claim 6 is characterized in that, described carrier is selected from any one or a few the mixture in zeolite, calcium carbonate, kaolin, the bentonite.
8. microorganism formulation according to claim 6 is characterized in that, described nutriment is selected from any one or a few the mixture in starch, molasses, trehalose, sucrose, maltose, isomaltose, wood sugar, fructose, stachyose, the gossypose.
9. the described microorganism formulation of any one according to claim 6-8, it is characterized in that, comprise carrier and nutriment, and take carrier and nutriment gross weight as benchmark, the percentage by weight of carrier is 80-90w%, and the percentage by weight of nutriment is 10-20wt%.
10. microorganism formulation according to claim 9 is characterized in that, described carrier is the combination of zeolite powder and calcium carbonate, and described nutriment is the combination of cornstarch and molasses; Wherein, take carrier and nutriment gross weight as benchmark, the zeolite powder percentage by weight is 65-75wt%, and weight of calcium carbonate percentage is 10-20wt%; The percentage by weight of cornstarch is 5-15wt%, and the percentage by weight of molasses is 5-15wt%.
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CN103749943A (en) * 2013-12-31 2014-04-30 吉林中粮生化有限公司 Method for producing biological feed by fermenting byproducts from deep processing of corns
CN103783261A (en) * 2013-10-15 2014-05-14 云南龙谷生物科技有限公司 Method for preparing probiotics and secondary metabolite for livestock through secondary microbial cultivation
CN104161227A (en) * 2014-08-28 2014-11-26 上海创博生态工程有限公司 Method for producing microorganism active complete feed for mutton sheep by using straws
CN106119171A (en) * 2016-08-17 2016-11-16 青岛千和农牧科技有限公司 The anti-microbial formulation of a kind of crop material feed fermentation and fermentation technology thereof
CN106615621A (en) * 2016-11-16 2017-05-10 山东众客食品有限公司 Feed additive for improving content of polyunsaturated fatty acid in livestock meat, as well as preparation method and application of feed additive
CN107279467A (en) * 2017-05-12 2017-10-24 正和清生命科技实业有限公司 A kind of complex microbial inoculum is used for the method for livestock-raising
CN109007267A (en) * 2018-08-23 2018-12-18 安徽农耕年华农业发展有限公司 A kind of feed producing method based on mushroom base-material
CN109095750A (en) * 2018-07-30 2018-12-28 开平市华声生物科技有限公司 A kind of processing method of livestock culture excreta
CN110915757A (en) * 2019-12-16 2020-03-27 内蒙古农业大学 Method for improving mutton quality by using lactobacillus feed
CN112790072A (en) * 2021-01-08 2021-05-14 江苏恒镇泰环保科技股份有限公司 Crop planting method for comprehensively improving agricultural land by utilizing microorganisms

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Publication number Priority date Publication date Assignee Title
CN103783261A (en) * 2013-10-15 2014-05-14 云南龙谷生物科技有限公司 Method for preparing probiotics and secondary metabolite for livestock through secondary microbial cultivation
CN103749943A (en) * 2013-12-31 2014-04-30 吉林中粮生化有限公司 Method for producing biological feed by fermenting byproducts from deep processing of corns
CN104161227A (en) * 2014-08-28 2014-11-26 上海创博生态工程有限公司 Method for producing microorganism active complete feed for mutton sheep by using straws
CN106119171A (en) * 2016-08-17 2016-11-16 青岛千和农牧科技有限公司 The anti-microbial formulation of a kind of crop material feed fermentation and fermentation technology thereof
CN106615621A (en) * 2016-11-16 2017-05-10 山东众客食品有限公司 Feed additive for improving content of polyunsaturated fatty acid in livestock meat, as well as preparation method and application of feed additive
CN107279467A (en) * 2017-05-12 2017-10-24 正和清生命科技实业有限公司 A kind of complex microbial inoculum is used for the method for livestock-raising
CN109095750A (en) * 2018-07-30 2018-12-28 开平市华声生物科技有限公司 A kind of processing method of livestock culture excreta
CN109095750B (en) * 2018-07-30 2021-12-31 开平市华声生物科技有限公司 Method for treating livestock breeding excrement
CN109007267A (en) * 2018-08-23 2018-12-18 安徽农耕年华农业发展有限公司 A kind of feed producing method based on mushroom base-material
CN110915757A (en) * 2019-12-16 2020-03-27 内蒙古农业大学 Method for improving mutton quality by using lactobacillus feed
CN112790072A (en) * 2021-01-08 2021-05-14 江苏恒镇泰环保科技股份有限公司 Crop planting method for comprehensively improving agricultural land by utilizing microorganisms

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Application publication date: 20131023