CN106359852A - Method for preparing alga feed by two-step fermentation - Google Patents
Method for preparing alga feed by two-step fermentation Download PDFInfo
- Publication number
- CN106359852A CN106359852A CN201610970210.2A CN201610970210A CN106359852A CN 106359852 A CN106359852 A CN 106359852A CN 201610970210 A CN201610970210 A CN 201610970210A CN 106359852 A CN106359852 A CN 106359852A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- sargassum
- feed
- algae
- seaweed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to a method for preparing an alga feed by two-step fermentation. The method comprises the following steps: pulverizing algae into particles with the diameter of 0.2cm or so, carrying out primary water washing for desalting, mixing the algae with bean dregs and bran, regulating the initial water content to 28-50%, respectively inoculating Bacillus licheniformis, Saccharomyces cerevisiae, Lactobacillus acidophilus and Enterococcus faecium, fermenting, and drying to obtain the finished product. The prepared alga fermented feed has the nutritive value of the algae; the alga macromolecules in the feed are degraded to some degree, so that the feed can be absorbed by animals more easily; the feed contains certain quantities of Bacillus licheniformis and metabolites of the strains; especially, the lactein has the function of probiotics; and therefore, the feed can keep the microecological balance of the livestock/poultry intestinal tracts and enhance the immunity.
Description
Technical field
The invention belongs to biological technical field is and in particular to a kind of two-step fermentation method of preparing seaweed fodder.
Background technology
Sargassum is a kind of nutritious living marine resources, is to carry out photosynthetic autophyte in ocean, presses
The be in different colours of its frond, can be divided into red algae, chlorella, Brown algae, cyanophyceae, chrysophyceae etc. again, various in style.Sea selected by the present invention
The entitled Alga Sgrgassi Enervess of algae, are one kind of Brown algae, and its protein content is 8%-12%, high up to 17%, contained alginate jelly, shallow lake
The no nitrogen organic such as powder, Mannitol and cellulose accounts for more than 30%, containing multivitamin, mineral, also contains some special
Composition, such as iodide, polyphenolic substance, phytosterin compound etc. promote the active substance of growth of animal.There are some researches show, Sargassum
There is lipid-loweringing, cholesterol reducing, anticancer antineoplastic effect, be widely used in industries such as food industry, medicine, secondly, from
Multiple medication chemistry product such as iodine, potassium, Algin, Mannitol, carrageenan, agar can also be extracted in Sargassum.Equally, by Sargassum
Very big with the potentiality of feed additive for feedstuff, it also can be a kind of preferable feed resource, but contains in Sargassum
Multiple anti-nutrition components, the such as non-starch polysaccharides(nsp) such as cellulose, carbohydrate gum, xylan, animal is non-digestible, just indirectly makes
Become waste, so the utilization rate how improving Sargassum is problem anxious to be resolved.
Content of the invention
For solving the above problems, the present invention using multi-cultur es two-step fermenting to ferment Sargassum be obtained active solid feedstuff,
Using strain be the Ministry of Agriculture announce allow in feedstuff safe handling strain, thalli growth metabolic process can be by Sargassum
Macromolecular substances be degraded to small-molecule substance, produce organic acid based on lactic acid, aminoacid, digestive enzyme, and remain sea
The composition such as contained natural growth hormone, vitamin, mineral, trace element in algae.
For achieving the above object, the technical scheme is that
A kind of method that two-step fermentation prepares seaweed fodder, its operating procedure is:
(1) wash desalination: Sargassum is ground into after 0.2cm square size, in mass ratio Sargassum weight: water is cleaned with water for 1:4-6
Filter off moisture after Sargassum, obtain filtering residue；
(2) seaweed fodder fermentation four kinds of seed liquor used are prepared；Its preparation method be by Bacillus licheniformis, saccharomyces cerevisiae,
Bacillus acidophilus, the inclined-plane lawn of enterococcus faecalis are respectively seeded in corresponding culture fluid and carry out seed culture；
(3) configure Sargassum solid medium；
(4) with composite bacteria liquid inoculation algae culture base fermentation；
(5) tunning is dried.
Wherein, the composition of described Sargassum solid medium mesostroma and proportioning are: by Sargassum filtering residue, bean dregs, wheat bran=7.5-
10:2-2.5:1-1.25, in terms of the percentage by weight of solid-state fermentation culture medium mesostroma, also contains following material: 0.5wt%-1
The glucose of wt %, the ammonium sulfate of 0.5 wt %-1 wt %, 0.5 wt %-1 wt % potassium dihydrogen phosphate, finally adjust water content
For 33%-48%.
The inoculation of described composite bacteria liquid and fermentation process are: first by Bacillus licheniformis and saccharomyces cerevisiae bacterium solution all with
The inoculum concentration of 2.1v/w%-2.7v/w% is inoculated in algae culture base, 30-32 DEG C of aerobic fermentation 24-30 hour, then inoculates
The bacillus acidophilus of 2.3 v/w %-3.3 v/w % inoculum concentrations and enterococcus faecalis bacterium solution are little in 35-37 DEG C of anaerobic fermentation 40-48
When.
The drying condition of described tunning is: 50-60 DEG C is dried 6-8 hour.
Finally give the finished product that moisture is 6%-10%.
The present invention has a following remarkable advantage:
The present invention chooses Bacillus licheniformis, saccharomyces cerevisiae, bacillus acidophilus and four kinds of bacterial strains of enterococcus faecalis and comes co-fermentation sea
Algae, has fully played the synergism between multi-cultur es, and the Bacillus licheniformis screening can be by fiber in alginic cell wall
Degraded, is conducive to itself and other bacterial strain to enter internal growth and Nutrition and Metabolism.Bacillus licheniformis are with saccharomyces cerevisiae first
Carry out aerobic culture, the carbon nitrogen source making full use of in culture medium carrys out synthesising thalli protein, and Bacillus licheniformis were being cultivated
Cheng Zhonghui produces 50% spore, and spore is stable hypopuss, and thermal resistance and acid-alkali resistance is high temperature resistant, and after product drying, survival rate is relatively
High.The lactic acid bacteria of last inoculating two kinds can produce lactic acid bacteria carrying out Anaerobic culturel, two kinds of lactic acid bacterias during growth metabolism
Element, lactein can suppress the propagation of harmful bacteria in intestinal, and after fermentation, ph can drop to 4.5-5.0.
Specific embodiment
Embodiment 1
A kind of method that two-step fermentation prepares seaweed fodder, its operating procedure is:
Step one, washing desalination
Sargassum be ground into 0.2cm about after size, Sargassum weight in mass ratio: water is that 1:4 water cleans and filters off moisture after Sargassum, obtains
To filtering residue.
Step 2, as fermentation of seaweed each seed liquor preparation
1) Bacillus licheniformis seed liquor preparation
Inoculate fluid medium with inclined-plane lawn, culture medium prescription is: 2 wt % Sargassums, 1 wt % peptone, 0.3 wt % cattle
Meat extract, 0.5 wt % sodium chloride, initial ph7.0,32 DEG C, 200 turns/min cultivates 20 hours.
2) saccharomyces cerevisiae seed liquor preparation
Inoculate fluid medium with inclined-plane lawn, culture medium prescription is: 3 wt % glucoses, 0.5 wt % peptone, 0.1 wt
% potassium dihydrogen phosphate, 0.1 wt % magnesium sulfate, 0.05 wt % potassium chloride, initial ph is natural, 30 DEG C, and 200 turns/min culture 20 is little
When.
3) bacillus acidophilus' seed liquor preparation
Inoculate fluid medium with inclined-plane lawn, culture medium prescription is: 2 wt % glucoses, 1 wt % peptone, 1 wt % cattle
Meat extract, 0.5 wt % yeast extract, 0.5 wt % anhydrous sodium acetate, 0.2 wt % ammonium citrate, 0.2 wt % potassium dihydrogen phosphate,
0.06 wt % magnesium sulfate, 0.02 wt % manganese sulfate, 0.1vol% Tween 80, initial ph6.2-6.6,37 DEG C, Anaerobic culturel 18 is little
When.
4) enterococcus faecalis seed liquor preparation
Inoculate fluid medium with inclined-plane lawn, culture medium prescription is: 2 wt % glucoses, 1 wt % peptone, 0.5 wt %
Carnis Bovis seu Bubali cream, 0.5 wt % anhydrous sodium acetate, 0.2 wt % ammonium citrate, 0.2 wt % potassium dihydrogen phosphate, 0.06 wt % sulphuric acid
Magnesium, 0.02 wt % manganese sulfate, 0.1vol% Tween 80, initial ph6.2-6.6,37 DEG C, Anaerobic culturel 21 hours.
Step 3, the preparation of Sargassum solid medium
By the Sargassum of filtered water, bean dregs, wheat bran by weight 8:2:1 mix homogeneously, and the weight by this solid medium siccative
Ratio adds the glucose of 0.5 wt %, the ammonium sulfate of 0.5 wt %, 0.5 wt % potassium dihydrogen phosphate, and finally adjustment water content is 35
v/w %.
Step 4, with composite bacteria liquid inoculation algae culture base fermentation
Bacillus licheniformis and saccharomyces cerevisiae bacterium solution are all inoculated in algae culture base with the inoculum concentration of 2.1v/w%, 32 DEG C good
Aerobe fermentation 24 hours, then inoculates bacillus acidophilus and the enterococcus faecalis bacterium solution of 3.3% inoculum concentration, in 37 DEG C of anaerobic fermentations 48
Hour.
Step 5, tunning are dried
After fermentation of seaweed product fermentations terminate, product is put 55 DEG C of oven dryings 7 hours, obtain the one-tenth that moisture is 6%-10%
Product.
Embodiment 2
A kind of method that two-step fermentation prepares seaweed fodder, its operating procedure is:
Step one, washing desalination
Sargassum be ground into 0.2cm about after size, Sargassum weight in mass ratio: water is that 1:5 water cleans and filters off moisture after Sargassum, obtains
To filtering residue.
Step 2, as fermentation of seaweed each seed liquor preparation
The preparation method of each seed liquor is as described in Example 1.
Step 3, the preparation of Sargassum solid medium
By the Sargassum of filtered water, bean dregs, wheat bran by weight 10:2.5:1 mix homogeneously, and the weight by this solid medium siccative
The glucose of amount ratio interpolation 1 wt %, the ammonium sulfate of 1 wt %, 1 wt % potassium dihydrogen phosphate, finally adjustment water content is 33 v/w
%.
Step 4, with composite bacteria liquid inoculation algae culture base fermentation
Bacillus licheniformis and saccharomyces cerevisiae bacterium solution are all inoculated in algae culture base with the inoculum concentration of 2.7v/w%, 30 DEG C good
Aerobe fermentation 30 hours, then inoculates bacillus acidophilus and the enterococcus faecalis bacterium solution of 2.3% inoculum concentration, in 35 DEG C of anaerobic fermentations 40
Hour.
Step 5, tunning are dried
After fermentation of seaweed product fermentations terminate, product is put 60 DEG C of oven dryings 6 hours, obtain the one-tenth that moisture is 6%-10%
Product.
Embodiment 3
A kind of method that two-step fermentation prepares seaweed fodder, its operating procedure is:
Step one, washing desalination
Sargassum be ground into 0.2cm about after size, Sargassum weight in mass ratio: water is that 1:6 water cleans and filters off moisture after Sargassum, obtains
To filtering residue.
Step 2, as fermentation of seaweed each seed liquor preparation
The preparation method of each seed liquor is as described in Example 1.
Step 3, the preparation of Sargassum solid medium
By the Sargassum of filtered water, bean dregs, wheat bran by weight 7.5:2:1 mix homogeneously, and the weight by this solid medium siccative
The glucose of amount ratio interpolation 0.8 wt %, the ammonium sulfate of 0.8 wt %, 0.8 wt % potassium dihydrogen phosphate, finally adjusting water content is
48v/w %.
Step 4, with composite bacteria liquid inoculation algae culture base fermentation
Bacillus licheniformis and saccharomyces cerevisiae bacterium solution are all inoculated in algae culture base with the inoculum concentration of 2.5v/w%, 31 DEG C good
Aerobe fermentation 26 hours, then inoculates bacillus acidophilus and the enterococcus faecalis bacterium solution of 3% inoculum concentration, little in 36 DEG C of anaerobic fermentations 45
When.
Step 5, tunning are dried
After fermentation of seaweed product fermentations terminate, product is put 50 DEG C of oven dryings 8 hours, obtain the one-tenth that moisture is 6%-10%
Product.
Claims (5)
1. a kind of two-step fermentation prepares the method for seaweed fodder it is characterised in that four kinds of bacterium solution used of fermenting are divided into two steps and are added
Enter in fermentation system.
2. as claimed in claim 1 a kind of two-step fermentation prepare seaweed fodder method it is characterised in that its concrete steps such as
Under:
(1) wash desalination: Sargassum is ground into after 0.2cm square size, in mass ratio Sargassum weight: water is cleaned with water for 1:4-6
Filter off moisture after Sargassum, obtain filtering residue；
(2) seaweed fodder fermentation four kinds of seed liquor used are prepared；
(3) configure Sargassum solid medium；
(4) with composite bacteria liquid inoculation algae culture base fermentation；
(5) tunning is dried.
3. a kind of two-step fermentation prepares the method for seaweed fodder it is characterised in that described Sargassum solid-state as claimed in claim 2
The composition of culture medium mesostroma and proportioning are: Sargassum filtering residue, bean dregs, wheat bran=7.5-10:2-2.5:1-1.25 are sent out with solid-state
The percentage by weight meter of ferment culture medium mesostroma, also contains following material: the glucose of 0.5wt%-1 wt %, 0.5 wt %-1
The ammonium sulfate of wt %, 0.5 wt %-1 wt % potassium dihydrogen phosphate, finally adjustment water content is 33%-48%.
4. a kind of two-step fermentation prepares the method for seaweed fodder it is characterised in that described compound bacteria as claimed in claim 2
Liquid inoculation and fermentation process are: first by Bacillus licheniformis and saccharomyces cerevisiae bacterium solution all with the inoculation of 2.1v/w%-2.7v/w%
Amount is inoculated in algae culture base, and then 30-32 DEG C of aerobic fermentation 24-30 hour inoculate 2.3 v/w %-3.3 v/w % and connect
Plant bacillus acidophilus and the enterococcus faecalis bacterium solution of amount, in 35-37 DEG C of anaerobic fermentation 40-48 hour.
5. a kind of two-step fermentation prepares the method for seaweed fodder it is characterised in that described tunning as claimed in claim 2
Drying condition be: 50-60 DEG C is dried 6-8 hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610970210.2A CN106359852A (en) | 2016-11-07 | 2016-11-07 | Method for preparing alga feed by two-step fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610970210.2A CN106359852A (en) | 2016-11-07 | 2016-11-07 | Method for preparing alga feed by two-step fermentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106359852A true CN106359852A (en) | 2017-02-01 |
Family
ID=57893883
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610970210.2A Pending CN106359852A (en) | 2016-11-07 | 2016-11-07 | Method for preparing alga feed by two-step fermentation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106359852A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107251989A (en) * | 2017-06-26 | 2017-10-17 | 内蒙古大学 | A kind of preparation method of the composite solid probiotics comprising Phellinus bacterium |
| CN107455605A (en) * | 2017-09-21 | 2017-12-12 | 佛山佳牧乐科技有限公司 | A kind of feed compound for being used to reduce cholesterol level of vitellus |
| CN109673815A (en) * | 2018-12-29 | 2019-04-26 | 嘉必优生物技术（武汉）股份有限公司 | Fermentation preparation is rich in the functional feed and preparation method of arachidonic acid and bata-carotene |
| CN110338278A (en) * | 2019-07-30 | 2019-10-18 | 威海长青海洋科技股份有限公司 | One main laminaria fermented feed containing probiotics and preparation method thereof |
| WO2022101397A1 (en) * | 2020-11-12 | 2022-05-19 | Fermentationexperts A/S | Process for providing a combination of fermented seaweed and/or algae and plant material selected from the family fabaceae |
| CN115736096A (en) * | 2022-11-22 | 2023-03-07 | 山东省海洋资源与环境研究院(山东省海洋环境监测中心、山东省水产品质量检验中心) | Preparation method and application of seaweed feed |
| WO2024115668A1 (en) * | 2022-12-01 | 2024-06-06 | Mahe Stephane | Process for preserving and transforming algae by lacto-fermentation, use of the lacto-fermented juice and algae obtained according to the process |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102987077A (en) * | 2012-12-26 | 2013-03-27 | 日照超凡生物技术有限公司 | Preparation method of seaweed fermented feed |
| CN104187436A (en) * | 2014-09-30 | 2014-12-10 | 青岛嘉瑞生物技术有限公司 | Preparation method of meat rabbit feed additive obtained through fermentation of seaweed and Chinese herbal medicines |
-
2016
- 2016-11-07 CN CN201610970210.2A patent/CN106359852A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102987077A (en) * | 2012-12-26 | 2013-03-27 | 日照超凡生物技术有限公司 | Preparation method of seaweed fermented feed |
| CN104187436A (en) * | 2014-09-30 | 2014-12-10 | 青岛嘉瑞生物技术有限公司 | Preparation method of meat rabbit feed additive obtained through fermentation of seaweed and Chinese herbal medicines |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107251989A (en) * | 2017-06-26 | 2017-10-17 | 内蒙古大学 | A kind of preparation method of the composite solid probiotics comprising Phellinus bacterium |
| CN107455605A (en) * | 2017-09-21 | 2017-12-12 | 佛山佳牧乐科技有限公司 | A kind of feed compound for being used to reduce cholesterol level of vitellus |
| CN109673815A (en) * | 2018-12-29 | 2019-04-26 | 嘉必优生物技术（武汉）股份有限公司 | Fermentation preparation is rich in the functional feed and preparation method of arachidonic acid and bata-carotene |
| CN109673815B (en) * | 2018-12-29 | 2022-08-09 | 嘉必优生物技术（武汉）股份有限公司 | Functional feed rich in arachidonic acid and beta carotene prepared by fermentation and preparation method thereof |
| CN110338278A (en) * | 2019-07-30 | 2019-10-18 | 威海长青海洋科技股份有限公司 | One main laminaria fermented feed containing probiotics and preparation method thereof |
| WO2022101397A1 (en) * | 2020-11-12 | 2022-05-19 | Fermentationexperts A/S | Process for providing a combination of fermented seaweed and/or algae and plant material selected from the family fabaceae |
| CN115736096A (en) * | 2022-11-22 | 2023-03-07 | 山东省海洋资源与环境研究院(山东省海洋环境监测中心、山东省水产品质量检验中心) | Preparation method and application of seaweed feed |
| WO2024115668A1 (en) * | 2022-12-01 | 2024-06-06 | Mahe Stephane | Process for preserving and transforming algae by lacto-fermentation, use of the lacto-fermented juice and algae obtained according to the process |
| FR3142655A1 (en) * | 2022-12-01 | 2024-06-07 | Stéphane MAHE | Process for preserving and transforming algae by lactofermentation, use of algae and lactofermented juices obtained according to the process. |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106359852A (en) | Method for preparing alga feed by two-step fermentation | |
| CN104829341B (en) | A kind of alga oligosaccharides Tiny ecosystem drip irrigation fertilizer and preparation method thereof | |
| CN105053537B (en) | It is a kind of using orange peel slag as the production method of the high protein feed of raw material and feed | |
| CN103829042B (en) | A kind of production method of polyvitmin active protein cassava feed | |
| CN104782892B (en) | A kind of sugarcane residue fermented feed and preparation method thereof | |
| CN104757267A (en) | Apple pomace microbial culture starter and method for producing biological feed by apple pomace microbial culture starter | |
| CN103859155A (en) | Refining method for probiotics-containing cassava feed | |
| CN103343104B (en) | Process for preparing novel plant-source probiotics solution | |
| CN105795098A (en) | Cassava dregs feed and preparation method thereof | |
| CN104106727A (en) | Complete fermented feed and preparing method thereof | |
| CN1324129C (en) | Multiple strain microorganism ferment production method and its uses in cow fine fodder | |
| CN111066947A (en) | Production method of clostridium butyricum feed additive | |
| CN110338278A (en) | One main laminaria fermented feed containing probiotics and preparation method thereof | |
| CN106889306A (en) | A kind of feed fermentation agent and the application on feed fermentation | |
| CN101836688B (en) | Preparation method of antibiotic-free microbial fermentation feed | |
| CN102669408A (en) | Method for producing feed through biologic improvement on compound grains | |
| CN111296723A (en) | Probiotic agent for multi-strain combined solid state fermentation of rapeseed meal and fermentation method thereof | |
| CN105494919A (en) | Yeast culture fermented by mixed bacteria and preparation method of yeast culture | |
| CN107853588A (en) | A kind of preparation method of feature highland barley flour | |
| CN104116000A (en) | Preparation method for fructo-oligo saccharide feed additive | |
| CN102940145A (en) | Preparation method of fermentation aquatic health care feed | |
| CN103392920B (en) | Fermentation method of soybean hulls | |
| CN106490307A (en) | A kind of method of antimicrobial composition fermentation Entermorpha | |
| CN116058432A (en) | Citric acid mycelium residue microbial feed additive and preparation method and application thereof | |
| CN102010857B (en) | Method for producing acid xylanase from whey powder |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170201 |
|
| RJ01 | Rejection of invention patent application after publication |