CN110338278A - One main laminaria fermented feed containing probiotics and preparation method thereof - Google Patents
One main laminaria fermented feed containing probiotics and preparation method thereof Download PDFInfo
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- CN110338278A CN110338278A CN201910694803.4A CN201910694803A CN110338278A CN 110338278 A CN110338278 A CN 110338278A CN 201910694803 A CN201910694803 A CN 201910694803A CN 110338278 A CN110338278 A CN 110338278A
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- kelp
- fermentation
- enzymatic hydrolysis
- fermented feed
- feed containing
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
Abstract
The invention belongs to field of fodder.The object of the present invention is to provide main laminaria fermented feed containing probiotics and preparation method thereof, it digests kelp using algin catenase in advance, make the intracorporal sodium alginate enzymatic hydrolysis of kelp at brown alga oligose, reduce the viscosity of fermentation raw material, permeability is improved, fermentation condition is improved, accesses probiotics after auxiliary material of arranging in pairs or groups in obtained kelp enzymolysis liquid, solid state fermentation 3-4 days, prepare the kelp fermented feed rich in brown alga monosaccharide and probiotics.Powder process mineral, the loss of the nutritional ingredients such as mannitol are dried and beaten the beneficial effects of the invention are as follows avoidable kelp；Pre- enzymatic hydrolysis is carried out before kelp fermentation, the substance enzymatic hydrolysis by the sodium alginate in kelp, the difficult consumption such as crude fibre is small molecular sugar, improves the digestibility of animal.The viscosity for reducing fermentation raw material simultaneously, the case where improving the gas permeability of fermentation raw material, prevent hot-spot common in solid state fermentation, thalli growth preferably.
Description
Technical field
The invention belongs to field of fodder, are related to main laminaria fermented feed containing probiotics and preparation method thereof.
Technical background
China is to propagate largest country artificially in the world.Kelp rich in a variety of amino acid, vitamin, minerals,
Microelement also contains many unidentified growth factors, is a kind of food with very high nutritive value.It has been investigated that sea
Band also have reducing blood lipid, it is hypoglycemic, adjust immune, anticoagulation, be antitumor, lead discharging removing toxic substances and a variety of biological functions such as anti-oxidant.
The large-scale farming of China kelp provides strong support for the development and utilization of kelp, using kelp fermented feed, not only may be used
To solve the problems, such as kelp excess production capacity, the raw material range of feed has also been widened.Since the dregs of beans reserves of present world market subtract
Few, industry development needs the feedstuff of high nutritive value to provide new power.
In the prior art, the fermentation of seaweeds product can not only lose big portion usually using Kelp Powder or kelp residue in this way
The nutritional ingredient divided, can also extend fermentation time.In notification number CN107865188A, raw material is done using Kelp Powder, due to it
Rich in algin, cause material viscosity excessively high, aeration is poor, and hot-spot in fermentation process needs artificial stirring, ferments simultaneously
Time is up to 20 days or more, and cost of labor and time cost are excessively high.And kelp residue fermented feed is used, the rock algae in kelp is more
Sugar, iodine, the solable matters such as mannitol have all lost, and nutrition substantially reduces.Since sodium alginate belongs to macromolecular sugar, it is difficult
It is decomposed by probiotics, therefore single-minded and high efficiency pretreatment is extremely important.
Summary of the invention
The object of the present invention is to provide main laminaria fermented feed containing probiotics and preparation method thereof, are cracked using algin
Enzyme digests kelp in advance, makes the intracorporal sodium alginate enzymatic hydrolysis of kelp at brown alga oligose, reduces the viscosity of fermentation raw material, improve
Permeability improves fermentation condition, and probiotics is accessed after auxiliary material of arranging in pairs or groups in obtained kelp enzymolysis liquid, solid state fermentation 3-4 days, is made
It is standby to go out to be rich in the kelp fermented feed of brown alga monosaccharide and probiotics.
For achieving the above object, the present invention adopts the following technical solutions are as follows: a main laminaria fermented feed containing probiotics,
The fermentation mixture access probiotics being made of enzymatic hydrolysis kelp liquid, dregs of beans, wheat bran, corn bran is characterized by through everfermentation
It forms.
Further, the weight percent of each ingredient is divided on the basis of the weight of kelp enzymolysis liquid in the fermentation mixture
Not are as follows: dregs of beans 25-40%, wheat bran 25-40%, corn bran 35-45%, the water content of the fermentation mixture is 40-50%.
Further, the probiotics refers to bacillus subtilis, bacillus licheniformis, saccharomycete, lactic acid bacteria.
It is extra large to enzymatic hydrolysis is obtained after kelp enzymatic hydrolysis to split enzyme using algin for the preparation method of one main laminaria fermented feed containing probiotics
Band liquid, then addition fermentation auxiliary material stirs evenly in enzymatic hydrolysis kelp liquid, and probiotics is accessed after sterilizing, system of fermenting in fermentation bag
At.
Further, the enzymatic hydrolysis kelp liquid, preparation method is to smash Fresh Laminaria Japonica, and 2-4 times of its weight is added
Clear water, the algin that weight 8-12% is then added splits enzyme, opens stirring, digest 2-3h, obtain enzymatic hydrolysis kelp liquid.
Further, the fermentation auxiliary material is dregs of beans, wheat bran and corn bran, each accounts for the weight percent of enzymatic hydrolysis kelp liquid
Than are as follows: dregs of beans 25-40%, wheat bran 25-40%, corn bran 35-45%, the water content of the fermentation mixture obtained after addition are 40-
50%。
Further, the probiotics is bacillus subtilis, bacillus licheniformis, saccharomycete, lactic acid bacteria, total access
Amount is the 3%-6% of fermentation mixture weight, wherein bacillus accounts for 20-25% in three kinds of mushrooms, saccharomycete accounts for 30-35%, lactic acid
Bacterium accounts for 40-50%.
Further, the fermentation termination condition in fermentation bag are as follows: bacillus amount >=1 × 107CFU/mL, yeast bacterial content
≥1×107CFU/mL, feed pH value≤4.5, crude fiber content≤10%, crude protein content improve >=3% before relatively fermenting, 5 items
Part is fermentation termination when meeting 3 or more, stops fermentation.
Through the above technical solution, the beneficial effects of the present invention are:
1. using fresh kelp as raw material, rather than used kelp residue or Kelp Powder are raw material in the prior art, are avoided
Powder process mineral, the loss of the nutritional ingredients such as mannitol are dried and beaten to kelp.Cumbersome stoving process is eliminated simultaneously, is dropped
Low hand labor cost.
2. pre- enzymatic hydrolysis has been carried out before kelp fermentation, by the sodium alginate in kelp, the substance enzymatic hydrolysis of the difficult consumption such as crude fibre
For small molecular sugar, the digestibility of animal is improved.The viscosity for reducing fermentation raw material simultaneously, improves the gas permeability of fermentation raw material,
The case where preventing hot-spot common in solid state fermentation, thalli growth preferably.
3. gained kelp fermented feed of the invention is rich in brown alga oligose, mannitol, the nutritional ingredients such as minerals.Be conducive to move
Object growth can promote animal milk production, and improve milk quality, improve immunity.A variety of organic acids are rich in after fermenting simultaneously in feed,
Animal intestinal tract pH value can be reduced, inhibits harmful bacteria growth, reduces animal intestinal tract disease, improve animal physique.
4. it is 3 to 4 days a length of when total production of gained kelp fermented feed containing probiotics of the invention, far below in the prior art 20
The time of it or so.For present invention gained feed crude fiber content less than 8%, sodium alginate etc. is difficult to the macromolecular absorbed sugar simultaneously
Content degradation rate is also 60% or more.
Specific embodiment
Fermented feed provided by the present invention and preparation method thereof is described in detail below with reference to embodiment.
Embodiment 1
The preparation step of one main laminaria fermented feed containing probiotics is as follows:
1) Fresh Laminaria Japonica is smashed, the clear water of 3 times of quality is added, add the algin catenase of 10% mass of people, opened stirring, stir
Revolving speed control is mixed between 180r/min-220r/min, temperature control viscosity of sludge after 30-35 DEG C, 30min-60min is obvious
It reduces, after continuing 2 ~ 3h of enzymatic hydrolysis, completes enzymatic hydrolysis, obtain enzymatic hydrolysis kelp liquid.
2) into the enzymatic hydrolysis kelp liquid that step 1) obtains addition fermentation auxiliary material, 1kg enzymatic hydrolysis kelp liquid correspond to 0.3kg dregs of beans,
0.3kg wheat bran, 0.4kg corn bran, regulation system water content are 50%, and enzymatic hydrolysis kelp liquid and fermentation auxiliary material are stirred evenly, obtained
Ferment mixture.
3) will ferment 121 DEG C of high pressure sterilizations of mixture, access bacillus subtilis, bacillus licheniformis, yeast after cooling
Bacterium, lactic acid bacteria.It is uniformly mixed, strain access amount is the 3% of fermentation mixture, wherein bacillus accounts for 25%, ferment in three kinds of mushrooms
Female bacterium accounts for 35%, and lactic acid bacteria accounts for 40%.
4) uniformly mixed material is fitted into fermentation bag, fermentation bag is furnished with one-way breather valve, ferments three days and arrives at 30 DEG C
Four days；Detect fermentation condition are as follows: bacillus amount 1.2 × 107CFU/mL, yeast bacterial content 1.3 × 107CFU/mL, feed pH value
4, crude fiber content≤9%, crude protein content improves 3% before relatively fermenting, and stops fermentation.
Obtain a main laminaria fermented feed containing probiotics through the above steps, the feed by enzymatic hydrolysis kelp liquid, dregs of beans, wheat bran,
The fermentation mixture access probiotics of corn bran composition forms through everfermentation.Wherein ferment mixture in each ingredient weight percent
Than being respectively as follows: dregs of beans 30%, wheat bran 30%, corn bran 40% on the basis of the weight of kelp enzymolysis liquid, the fermentation mixture contains
Water is 50%.
Feed (1) and tradition obtained by preparation method provided in this embodiment use spontaneous fermentation using Kelp Powder, kelp residue
Feed (2) the inspection data comparison of acquisition is as follows:
Test, test period are compared on pig farm using feed (1) obtained by the present embodiment preparation method and conventional feed (2)
1.5 months, Experimental comparison results were as follows:
By above-mentioned comparative test as it can be seen that kelp fermented feed containing probiotics and conventional feed provided herein are more in rock algae
Sugar, iodine all increase significantly on mannitol content, and crude fiber content is reduced, on feeding effect, it may have apparent
It is promoted.
Embodiment 2
The preparation step of one main laminaria fermented feed containing probiotics is as follows:
1) Fresh Laminaria Japonica is smashed, the clear water of 2 times of quality is added, add the algin catenase of 8% mass of people, open stirring, stirring
Revolving speed controls between 180r/min-220r/min, and temperature control viscosity of sludge after 30-35 DEG C, 30min-60min obviously drops
It is low, after continuing 2 ~ 3h of enzymatic hydrolysis, enzymatic hydrolysis is completed, obtains enzymatic hydrolysis kelp liquid.
2) into the enzymatic hydrolysis kelp liquid that step 1) obtains addition fermentation auxiliary material, 1kg enzymatic hydrolysis kelp liquid correspond to 0.25kg dregs of beans,
0.4kg wheat bran, 0.35kg corn bran, regulation system water content are 40%, and enzymatic hydrolysis kelp liquid and fermentation auxiliary material are stirred evenly, obtained
To fermentation mixture.
3) will ferment 121 DEG C of high pressure sterilizations of mixture, access bacillus subtilis, bacillus licheniformis, yeast after cooling
Bacterium, lactic acid bacteria.It is uniformly mixed, strain access amount is the 5% of fermentation mixture, wherein bacillus accounts for 20%, ferment in three kinds of mushrooms
Female bacterium accounts for 30%, and lactic acid bacteria accounts for 50%.
4) uniformly mixed material is fitted into fermentation bag, fermentation bag is furnished with one-way breather valve, ferments three days and arrives at 30 DEG C
Four days；Detect fermentation condition are as follows: bacillus amount 1.15 × 107CFU/mL, yeast bacterial content 1.26 × 107CFU/mL, feed pH
Value 4.5, crude fiber content 9.6%, crude protein content improve 3.6% before relatively fermenting, stop fermentation.
Obtain a main laminaria fermented feed containing probiotics through the above steps, the feed by enzymatic hydrolysis kelp liquid, dregs of beans, wheat bran,
The fermentation mixture access probiotics of corn bran composition forms through everfermentation.Wherein ferment mixture in each ingredient weight percent
Than being respectively as follows: dregs of beans 25%, wheat bran 40%, corn bran 35% on the basis of the weight of kelp enzymolysis liquid, the fermentation mixture contains
Water is 40%.
Feed (1) and tradition obtained by preparation method provided in this embodiment use spontaneous fermentation using Kelp Powder, kelp residue
Feed (2) the inspection data comparison of acquisition is as follows:
Test, test period are compared on pig farm using feed (1) obtained by the present embodiment preparation method and conventional feed (2)
1.5 months, Experimental comparison results were as follows:
By above-mentioned comparative test as it can be seen that kelp fermented feed containing probiotics and conventional feed provided herein are more in rock algae
Sugar, iodine all increase significantly on mannitol content, and crude fiber content is reduced, on feeding effect, it may have apparent
It is promoted.
Embodiment 3
The preparation step of one main laminaria fermented feed containing probiotics is as follows:
1) Fresh Laminaria Japonica is smashed, the clear water of 4 times of quality is added, add the algin catenase of 12% mass of people, opened stirring, stir
Revolving speed control is mixed between 180r/min-220r/min, temperature control viscosity of sludge after 30-35 DEG C, 30min-60min is obvious
It reduces, after continuing 2 ~ 3h of enzymatic hydrolysis, completes enzymatic hydrolysis, obtain enzymatic hydrolysis kelp liquid.
2) into the enzymatic hydrolysis kelp liquid that step 1) obtains addition fermentation auxiliary material, 1kg enzymatic hydrolysis kelp liquid correspond to 0.4kg dregs of beans,
0.25kg wheat bran, 0.35kg corn bran, regulation system water content are 45%, and enzymatic hydrolysis kelp liquid and fermentation auxiliary material are stirred evenly, obtained
To fermentation mixture.
3) will ferment 121 DEG C of high pressure sterilizations of mixture, access bacillus subtilis, bacillus licheniformis, yeast after cooling
Bacterium, lactic acid bacteria.It is uniformly mixed, strain access amount is the 6% of fermentation mixture, wherein bacillus accounts for 22%, ferment in three kinds of mushrooms
Female bacterium accounts for 33%, and lactic acid bacteria accounts for 45%.
4) uniformly mixed material is fitted into fermentation bag, fermentation bag is furnished with one-way breather valve, ferments three days and arrives at 30 DEG C
Four days；Detect fermentation condition are as follows: bacillus amount 1.05 × 107CFU/mL, yeast bacterial content 1.16 × 107CFU/mL, feed pH
Value 4.6, crude fiber content 8.7%, crude protein content improve 3.1% before relatively fermenting, stop fermentation.
Obtain a main laminaria fermented feed containing probiotics through the above steps, the feed by enzymatic hydrolysis kelp liquid, dregs of beans, wheat bran,
The fermentation mixture access probiotics of corn bran composition forms through everfermentation.Wherein ferment mixture in each ingredient weight percent
Than being respectively as follows: dregs of beans 40%, wheat bran 25%, corn bran 35% on the basis of the weight of kelp enzymolysis liquid, the fermentation mixture contains
Water is 45%.
Feed (1) and tradition obtained by preparation method provided in this embodiment use spontaneous fermentation using Kelp Powder, kelp residue
Feed (2) the inspection data comparison of acquisition is as follows:
Test, test period are compared on pig farm using feed (1) obtained by the present embodiment preparation method and conventional feed (2)
1.5 months, Experimental comparison results were as follows:
By above-mentioned comparative test as it can be seen that kelp fermented feed containing probiotics and conventional feed provided herein are more in rock algae
Sugar, iodine all increase significantly on mannitol content, and crude fiber content is reduced, on feeding effect, it may have apparent
It is promoted.
Claims (8)
1. a main laminaria fermented feed containing probiotics, it is characterised in that: by being made of enzymatic hydrolysis kelp liquid, dregs of beans, wheat bran, corn bran
Fermentation mixture access probiotics formed through everfermentation.
2. main laminaria fermented feed containing probiotics according to claim 1, it is characterised in that: each in the fermentation mixture
The weight percent of ingredient is respectively as follows: dregs of beans 25-40%, wheat bran 25-40%, corn bran on the basis of the weight of kelp enzymolysis liquid
The water content of 35-45%, the fermentation mixture are 40-50%.
3. main laminaria fermented feed containing probiotics according to claim 1, it is characterised in that: the probiotics refers to withered grass
Bacillus, bacillus licheniformis, saccharomycete, lactic acid bacteria.
4. a kind of preparation method of kelp fermented feed containing probiotics described in claim 1-3, it is characterised in that: utilize algin
Enzyme is split to enzymatic hydrolysis kelp liquid is obtained after kelp enzymatic hydrolysis, and then addition fermentation auxiliary material stirs evenly in enzymatic hydrolysis kelp liquid, after sterilizing
Probiotics is accessed, ferments and is made in fermentation bag.
5. the preparation method of main laminaria fermented feed containing probiotics according to claim 4, it is characterised in that: the enzyme
Kelp liquid is solved, preparation method is to smash Fresh Laminaria Japonica, and the clear water of 2-4 times of its weight is added, and is then added weight 8-12%'s
Algin splits enzyme, opens stirring, digests 2-3h, obtains enzymatic hydrolysis kelp liquid.
6. the preparation method of main laminaria fermented feed containing probiotics according to claim 4, it is characterised in that: the fermentation
Auxiliary material is dregs of beans, wheat bran and corn bran, each accounts for the weight percent of enzymatic hydrolysis kelp liquid are as follows: dregs of beans 25-40%, wheat bran 25-
40%, corn bran 35-45%, the water content of the fermentation mixture obtained after addition are 40-50%.
7. the preparation method of main laminaria fermented feed containing probiotics according to claim 4, it is characterised in that: described prebiotic
Bacterium is bacillus subtilis, bacillus licheniformis, saccharomycete, lactic acid bacteria, and total access amount is the 3%- of fermentation mixture weight
6%, wherein bacillus accounts for 20-25% in three kinds of mushrooms, saccharomycete accounts for 30-35%, and lactic acid bacteria accounts for 40-50%.
8. the preparation method of a main laminaria fermented feed containing probiotics according to claim 4 or 7, it is characterised in that: fermentation
Fermentation termination condition in bag are as follows: bacillus amount >=1 × 107CFU/mL, yeast bacterial content >=1 × 107CFU/mL, feed pH
Value≤4.5, crude fiber content≤10%, crude protein content improve >=3% before relatively fermenting, 5 conditions meet 3 and the above Shi Weifa
Ferment terminal stops fermentation.
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Cited By (4)
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| CN110771753A (en) * | 2019-12-06 | 2020-02-11 | 江苏海洋大学 | Method for developing shellfish feed by using waste laver |
| CN111034994A (en) * | 2019-11-18 | 2020-04-21 | 福建卫生职业技术学院 | Preparation method of functional fermented laminarin |
| CN113016954A (en) * | 2021-04-19 | 2021-06-25 | 山东新希望六和集团有限公司 | Fermented feed capable of improving pork quality and preparation method thereof |
| CN114504065A (en) * | 2022-03-09 | 2022-05-17 | 湖北丰甜生物科技有限公司 | Method for producing natural bait low-temperature composite algae for aquatic animals |
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Application publication date: 20191018 |