CN107439795A - A kind of feed addictive and its preparation technology containing compound marine alga - Google Patents
A kind of feed addictive and its preparation technology containing compound marine alga Download PDFInfo
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- CN107439795A CN107439795A CN201710517531.1A CN201710517531A CN107439795A CN 107439795 A CN107439795 A CN 107439795A CN 201710517531 A CN201710517531 A CN 201710517531A CN 107439795 A CN107439795 A CN 107439795A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
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Abstract
The invention discloses a kind of feed addictive containing compound marine alga, product contains brown alga carbohydrate and green alga carbohydrate and yeast cell wall, chitosan isoreactivity material, the pollution of a variety of mycotoxins such as aflatoxin in the feedstuffs such as corn, cereal and feed, zearalenone, T2 toxin, vomitoxin and fumonisin can be effectively removed after compounding, prevent livestock and poultry from triggering various diseases because of the feed of domestic fungus endotoxin contamination, animal health is ensured, improves productivity effect.The product advantage is:First, the harm of mycotoxin can be prevented comprehensively;Second, each component plays synergy, detoxification is strong, and is not easy to desorb after detoxification, and it is more thorough to remove toxin;Third, detoxification efficiency high, dosage are few;Fourth, high specificity, acts only on mycotoxin, nutrient is not adsorbed, does not influence livestock and poultry normal growth;Fifth, immune system can be strengthened, fowl poultry immune function is improved, strengthens body resistance against diseases.
Description
Technical field
The present invention relates to a kind of feed addictive containing compound marine alga and its preparation and application method, belong to feed addictive
Field.
Background technology
Investigation from global, national and local provinces and cities shows that Mycotoxins in Feed pollution is very universal and tight
Weight.There are mycotoxin contamination in all kinds of feedstuffs, all kinds of compound feed products, each regional, each season, and recall rate can be high
Up to 70%~100%, the toxin exceeding standard rate judged according to forage health standard is up to 20%~80%.Pollute the detection poison of feed
Plain 2~3 kinds of person, more 7~8 kinds of persons less, the feature of a variety of mycotoxin combined pollutions is presented.Mycotoxin passes through directly or indirectly
Function influence livestock product safety.Directly effect is that mycotoxin remains in the animal foods such as meat, egg, milk, and indirectly-acting is
Declined by the animal disease resistant power of mycotoxin contamination, antibiotic dosage increase, improve the risk that antibiotic remains in livestock products.
According to FAO (Food and Agriculture Organization of the United Nation)(FAO)Data, the crops in the whole world annual about 25% are to some extent by the dirt of mycotoxin
Dye, the crops that there are about 2% lose feeding value because seriously polluted.
The harm of mycotoxin slow poisoning be destroy animal immunologic function, reduce to stress and cause of disease resistance,
Reduce the protective rate of vaccine.Animal thymus depauperation and atrophy can be caused by eating mycotoxin, suppress Complement C4 and lymph is thin
Born of the same parents produce, and reduce cytokine secretion, and the mycotoxin of high dose can directly result in immunosupress.Therefore, there is mould malicious
In the case of element pollution, animal once has pathogenic microorganism invasion and attack, it is easy to fulminant disease.
At present, the prevention and controls optimal to mycotoxin are to add to take off mould dose in feed.Conventional de- mould dose of species has
Clay class, yeast cell wall class, microbiology class, enzymolysis class and Chinese herbal medicine etc..We have found that simultaneously deep processing obtains for extraction from marine alga
The brown alga carbohydrate and green alga carbohydrate obtained also has the function that effectively to contain that mycotoxin produces murder by poisoning to body, by these algaes
Carbohydrate has synergistic effect when being used together for above-mentioned several de- mould doses.Although also there are some composite parts on Vehicles Collected from Market
It is de- mould dose, but also differ bigger without corresponding national examination criteria, using effect.Therefore develop it is a kind of it is more novel,
Efficiently more it is necessary with safe de- mould dose.
The content of the invention
It is an object of the invention to provide a kind of feed addictive containing compound marine alga, can effectively remove corn, cereal etc.
A variety of mould poison such as aflatoxin, zearalenone, T2 toxin, vomitoxin and fumonisin in feedstuff and feed
The pollution of element, prevents livestock and poultry from triggering various diseases because of the feed of domestic fungus endotoxin contamination, ensures animal health, improves production
Benefit.
The purpose of the present invention is achieved by the following technical solution:
The present invention provides a kind of feed addictive containing compound marine alga, it by brown alga carbohydrate, green alga carbohydrate, yeast cell wall, change
Property montmorillonite, chitosan, calcium formate, the concave convex rod based on palygorskite mineral, sepiolite, hydration sodium calcium aluminium silicate, copper sulphate,
Sodium salt and B B-complex composition.
Wherein, the weight proportion of above-mentioned raw materials is as follows:Brown alga carbohydrate 1-40 parts, green alga carbohydrate 1-40 parts, yeast cell wall
1-40 parts, modified montmorillonite used 10-60 parts, chitosan 1-15 parts, calcium formate 1-40 parts, the concave convex rod 0.1- based on palygorskite mineral
40 parts, sepiolite 0.1-40 parts, hydration sodium calcium aluminium silicate 0.1-40 parts, copper sulphate 0.01-10 parts, sodium salt 0.01-10 parts and
B B-complex 4-60 parts, the B B-complex are made up of following weight parts component:Vitamin A 1-50 parts, vitamin E 1-
50 parts, vitamin C 1-30 parts and yeast selenium 1-50 parts.
Further, the weight proportion of above-mentioned raw materials is as follows:Brown alga carbohydrate 10-30 parts, green alga carbohydrate 5-20 parts, yeast cells
Wall 1-25 parts, modified montmorillonite used 18-35 parts, chitosan 5-10 parts, calcium formate 5-30 parts, the concave convex rod based on palygorskite mineral
0.1-30 parts, sepiolite 0.1-20 parts, hydration sodium calcium aluminium silicate 0.1-20 parts, copper sulphate 0.01-5 parts, sodium salt 0.01-6 parts
With B B-complex 5-50 parts, the B B-complex is made up of following weight parts component:Vitamin A 5-40 parts, vitamin E
10-50 parts, vitamin C 5-30 parts and yeast selenium 15-50 parts.
Another step, the weight proportion of above-mentioned raw materials are:Brown alga carbohydrate 15-30 parts, green alga carbohydrate 10-20 parts, yeast cells
Wall 10-25 parts, modified montmorillonite used 18-30 parts, chitosan 5-8 parts, calcium formate 5-20 parts, the concave convex rod based on palygorskite mineral
0.1-20 parts, sepiolite 5-20 parts, hydration sodium calcium aluminium silicate 5-20 parts, copper sulphate 0.01-3 parts, sodium salt 0.01-4 parts and multiple
Vitamin 5-35 parts are closed, the B B-complex is made up of following weight parts component:Vitamin A 5-30 parts, vitamin E 10-
40 parts, vitamin C 10-30 parts and yeast selenium 15-40 parts.
Further, the weight proportion of above-mentioned raw materials is:20 parts of brown alga carbohydrate, 16 parts of green alga carbohydrate, yeast cell wall 10
1 part of concave convex rod, 5 parts of sepiolite, water based on part, modified montmorillonite used 25 parts, 5 parts of chitosan, 5 parts of calcium formate, palygorskite mineral
5 parts of 5 parts of sodium calcium aluminium silicate, 1.5 parts of copper sulphate, 1.5 parts of sodium salt and B B-complex are closed, the B B-complex is by following heavy
Measure part component composition:15 parts of 30 parts of vitamin A, 25 parts of vitamin E, 30 parts of vitamin C and yeast selenium.
Above-mentioned brown alga carbohydrate is made by the steps:Using brown alga powder as raw material, 12-18 times of water for adding its weight stirs
Mix uniformly, boil 10-15min, cool, after 28 DEG C are incubated 25-40min, add specific bacterial strain Pseudomonas.
The algin catenase of sp.HZJ216 productions, stirs evenly, homogenate is put into 28 DEG C of insulating boxs and digests 3h, the addition of its enzyme
For the 0.05-0.08% of homogenate weight, in course of reaction otherwise stop stirring, boil enzymolysis reaction, produced after spraying drying
Finished product.
The brown alga is one or more of mixtures in sea-tangle, opotism, sargassum and bulk kelp.
It is prepared by above-mentioned green alga carbohydrate:The chlorella powder washed, dry, crushed is pressed into solid-liquid ratio 1:35 add water, and 90-100 DEG C carries
Take 4h, it is cold go after, add green algae polysaccharide digestive enzyme in the solution, while add Mg2+In 40-50 DEG C, in pH value in 7.5-8.5 models
Interior enzymolysis 0.5-2h is enclosed, after enzymolysis terminates, is heated to the 2-3min that seethes with excitement and be incubated, after cooling, sodium dihydrogen phosphate is added, adjusts pH
To 7.0 or so, spraying is dried.
The green alga is one or more of mixtures in Enteromorpha, sea lettuce, reef film.
The feed addictive of the present invention is used for the feedstuff such as corn, cereal and mixed feed by mycotoxin contamination
De- mycotoxin, the various diseases for preventing livestock and poultry from triggering by feeding mycotoxin contamination feed.
Present invention also offers the preparation method of the feed addictive, specifically comprise the following steps:
(1)Weigh various vitamins respectively by weight ratio:Vitamin A, vitamin E, vitamin and yeast selenium, carry out uniformly,
As B B-complex;
(2)Respectively by the concave convex rod based on brown alga carbohydrate, green alga carbohydrate, yeast cell wall, modified montmorillonite used, palygorskite mineral,
Sepiolite and hydration sodium calcium aluminium silicate carry out ultramicro grinding, and powder weighs fucose respectively again by weight by 200 mesh sieves
Class, green alga carbohydrate, yeast cell wall, modified montmorillonite used, chitosan, calcium formate, the concave convex rod based on palygorskite mineral, Hai Pao
Stone, hydration sodium calcium aluminium silicate, copper sulphate, sodium salt and B B-complex are well mixed and produced.
The invention provides the application process of this feed addictive, it is characterised in that the adding proportion in feed is 1-
2kg/T。
Integrated application absorption class material, organic acid and vitamins raw material of the present invention, coordinate high-efficient cleaning except mould by reaching
Verticillium toxin and its effect of influence, absorption class material can be with strong adsorption mycotoxin;Organic acid can create sour environment, suppression
Mildew making grows;A vitamin antagonist synthesizes, and T cell increment, monocyte phagocytosis function is all indispensable, can be antiviral, resists
Tumour, suppress the formation of nitroso compound, improve the immunity of body, there is protection for liver and propagating system and repair
Function, build up health, there is mitigation for the injury that toxin is brought.
Each component and its dosage in the feed addictive of the present invention are that this team is got by largely repeatedly practising, this
The feed addictive of invention, internal feed raw material and Mycotoxins in Feed pollution condition are directed on prescription, using fucose
Class, green alga carbohydrate, modified montmorillonite used, yeast cell wall and chitosan can be removed effectively in the feedstuffs such as corn, cereal, feed
The pollution of a variety of mycotoxins such as aflatoxin, zearalenone, T2 toxin, vomitoxin, meanwhile, protect Animal Liver
Kidney, promote toxin discharge, repair damaged tissues organ, improve immunity, build up health, in effectively preventing livestock and poultry mycotoxin
The generation of poison and relevant disease, ensure health, the safety of cultivated animals.The advantages and positive effects of the present invention are:
(1)The feed addictive of the present invention is compound de- mould dose, can prevent the harm of mycotoxin comprehensively.On the one hand can effectively go
A variety of mould poison such as zearalenone, vomitoxin, T2 toxin, aflatoxin B1 and fumarubicin in depollution feed
Element, prevent the absorption of mycotoxin in vivo;On the other hand it can promote to come into the mycotoxin discharge in animal body,
Avoid the harm of mycotoxin;
(2)The feed addictive each component of the present invention plays synergy, and detoxification is strong, and is not easy to desorb after detoxification, removes
Toxin is more thorough;
(3)The feed addictive detoxification efficiency high of the present invention, dosage are few;
(4)The feed addictive high specificity of the present invention, acts only on mycotoxin, does not adsorb nutrient, do not influence livestock and poultry
Normal growth;
(5)The feed of the present invention can also strengthen immune system with de- mould dose, improve fowl poultry immune function, strengthen body disease-resistant energy
Power.
Embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.Although with reference to the foregoing embodiments
, still can be to previous embodiment to being described in detail by invention, but for the person of ordinary skill of the art
Described technical scheme is modified, or carries out equivalent substitution to which part technical characteristic;And to these modification or
Replace, the essence of appropriate technical solution is departed from the spirit and scope of claimed technical solution of the invention.
Embodiment 1
A kind of feed addictive containing compound marine alga, is made by the steps:
(1)It is prepared by brown alga carbohydrate:Using Algemin as raw material, 12-18 times of water for adding its weight stirs, and boils 10-
15min, cool, after 28 DEG C are incubated 25-40min, add specific bacterial strain Pseudomonas. sp.HZJ216 productions
Algin catenase, stir evenly, homogenate is put into 28 DEG C of insulating boxs and digests 3h, the addition of its enzyme is homogenate weight
0.05-0.08%, in course of reaction otherwise stop stirring, boil enzymolysis reaction, got product after spraying drying;
(2)It is prepared by green alga carbohydrate:The ulva lactuca powder washed, dry, crushed is pressed into solid-liquid ratio 1:35 add water, and 90-100 DEG C is extracted 4h,
It is cold go after, add green algae polysaccharide digestive enzyme in the solution, while add Mg2+In 40-50 DEG C, in pH value in the range of 7.5-8.5
0.5-2h is digested, after enzymolysis terminates, is heated to the 2-3min that seethes with excitement and be incubated, after cooling, adds sodium dihydrogen phosphate, adjustment pH is extremely
7.0 or so, spraying drying;
(3)It is prepared by vitamin:10 parts of 30 parts of vitamin A, 25 parts of vitamin E, 20 parts of vitamin and yeast selenium are weighed, is carried out
It is well mixed, as B B-complex, it is standby;
(4)Crush:By the concave convex rod based on brown alga carbohydrate, green alga carbohydrate, yeast cell wall, modified montmorillonite used, palygorskite mineral,
Sepiolite and hydration sodium calcium aluminium silicate carry out ultramicro grinding, and powder is standby by 200 mesh sieves;
(5)Mixing:Respectively by 20 parts of brown alga carbohydrate, 25 parts of green alga carbohydrate, 12 parts of yeast cell wall, modified montmorillonite used 30 parts, shell
2 parts of glycan, 2 parts of calcium formate, 0.5 part of concave convex rod based on palygorskite mineral, 0.5 part of sepiolite, hydration sodium calcium aluminium silicate 0.4
Part, 0.2 part of copper sulphate, 2.4 parts of sodium salt and 5 parts of ratios of B B-complex weigh raw material, are well mixed, produce.
Embodiment 2
A kind of feed addictive containing compound marine alga, is made by the steps:
(1)It is prepared by brown alga carbohydrate:Using opotism as raw material, 12-18 times of water for adding its weight stirs, and boils 10-
15min, cool, after 28 DEG C are incubated 25-40min, add specific bacterial strain Pseudomonas. sp.HZJ216 productions
Algin catenase, stir evenly, homogenate is put into 28 DEG C of insulating boxs and digests 3h, the addition of its enzyme is homogenate weight
0.05-0.08%, in course of reaction otherwise stop stirring, boil enzymolysis reaction, got product after spraying drying;
(2)It is prepared by green alga carbohydrate:The enteromorpha powder washed, dry, crushed is pressed into solid-liquid ratio 1:35 add water, and 90-100 DEG C is extracted 4h,
It is cold go after, add green algae polysaccharide digestive enzyme in the solution, while add Mg2+In 40-50 DEG C, in pH value in the range of 7.5-8.5
0.5-2h is digested, after enzymolysis terminates, is heated to the 2-3min that seethes with excitement and be incubated, after cooling, adds sodium dihydrogen phosphate, adjustment pH is extremely
7.0 or so, spraying drying;
(3)It is prepared by vitamin:8 parts of 20 parts of vitamin A, 30 parts of vitamin E, 22 parts of vitamin C and yeast selenium are weighed, is carried out
It is well mixed, as B B-complex, it is standby;
(4)Crush:By the concave convex rod based on brown alga carbohydrate, green alga carbohydrate, yeast cell wall, modified montmorillonite used, palygorskite mineral,
Sepiolite and hydration sodium calcium aluminium silicate carry out ultramicro grinding, and powder is standby by 200 mesh sieves;
(5)Mixing:Respectively by 22 parts of brown alga carbohydrate, 20 parts of green alga carbohydrate, 15 parts of yeast cell wall, modified montmorillonite used 35 parts, shell
3 parts of glycan, 3 parts of calcium formate, 0.1 part of concave convex rod based on palygorskite mineral, 0.1 part of sepiolite, hydration sodium calcium aluminium silicate 0.1
Part, 0.5 part of copper sulphate, 3 parts of sodium salt and 8 parts of ratios of B B-complex weigh raw material, are well mixed, produce.
Embodiment 3
A kind of feed addictive containing compound marine alga, is made by the steps:
(1)It is prepared by brown alga carbohydrate:Using sargassum powder as raw material, 12-18 times of water for adding its weight stirs, and boils 10-
15min, cool, after 28 DEG C are incubated 25-40min, add specific bacterial strain Pseudomonas. sp.HZJ216 productions
Algin catenase, stir evenly, homogenate is put into 28 DEG C of insulating boxs and digests 3h, the addition of its enzyme is homogenate weight
0.05-0.08%, in course of reaction otherwise stop stirring, boil enzymolysis reaction, got product after spraying drying;
(2)It is prepared by green alga carbohydrate:The ulva lactuca powder washed, dry, crushed is pressed into solid-liquid ratio 1:35 add water, and 90-100 DEG C is extracted 4h,
It is cold go after, add green algae polysaccharide digestive enzyme in the solution, while add Mg2+In 40-50 DEG C, in pH value in the range of 7.5-8.5
0.5-2h is digested, after enzymolysis terminates, is heated to the 2-3min that seethes with excitement and be incubated, after cooling, adds sodium dihydrogen phosphate, adjustment pH is extremely
7.0 or so, spraying drying;
(3)It is prepared by vitamin:9 parts of 25 parts of vitamin A, 35 parts of vitamin E, 25 parts of vitamin C and yeast selenium are weighed, is carried out
It is well mixed, as B B-complex, it is standby;
(4)Crush:By the concave convex rod based on brown alga carbohydrate, green alga carbohydrate, yeast cell wall, modified montmorillonite used, palygorskite mineral,
Sepiolite and hydration sodium calcium aluminium silicate carry out ultramicro grinding, and powder is standby by 200 mesh sieves;
(5)Mixing:Gather respectively by 22 parts of brown alga carbohydrate, 28 parts of green alga carbohydrate, 8 parts of yeast cell wall, modified montmorillonite used 35 parts, shell
0.1 part of concave convex rod, 0.1 part of sepiolite, hydration sodium calcium aluminium silicate 0.1 based on sugared 2 parts, 5 parts of calcium formate, palygorskite mineral
Part, 0.5 part of copper sulphate, 3 parts of sodium salt and 15 parts of ratios of B B-complex weigh raw material, are well mixed, produce.
Embodiment 4
A kind of feed addictive containing compound marine alga, is made by the steps:
(1)It is prepared by brown alga carbohydrate:Using Kelp Powder as raw material, 12-18 times of water for adding its weight stirs, and boils 10-
15min, cool, after 28 DEG C are incubated 25-40min, add specific bacterial strain Pseudomonas. sp.HZJ216 productions
Algin catenase, stir evenly, homogenate is put into 28 DEG C of insulating boxs and digests 3h, the addition of its enzyme is homogenate weight
0.05-0.08%, in course of reaction otherwise stop stirring, boil enzymolysis reaction, got product after spraying drying;
(2)It is prepared by green alga carbohydrate:The ulva lactuca powder washed, dry, crushed is pressed into solid-liquid ratio 1:35 add water, and 90-100 DEG C is extracted 4h,
It is cold go after, add green algae polysaccharide digestive enzyme in the solution, while add Mg2+In 40-50 DEG C, in pH value in the range of 7.5-8.5
0.5-2h is digested, after enzymolysis terminates, is heated to the 2-3min that seethes with excitement and be incubated, after cooling, adds sodium dihydrogen phosphate, adjustment pH is extremely
7.0 or so, spraying drying;
(3)It is prepared by vitamin:15 parts of 30 parts of vitamin A, 35 parts of vitamin E, 30 parts of vitamin C and yeast selenium are weighed, is carried out
It is well mixed, as B B-complex, it is standby;
(4)Crush:By the concave convex rod based on brown alga carbohydrate, green alga carbohydrate, yeast cell wall, modified montmorillonite used, palygorskite mineral,
Sepiolite and hydration sodium calcium aluminium silicate carry out ultramicro grinding, and powder is standby by 200 mesh sieves;
(5)Mixing:Gather respectively by 30 parts of brown alga carbohydrate, 35 parts of green alga carbohydrate, 1 part of yeast cell wall, modified montmorillonite used 30 parts, shell
0.1 part of concave convex rod, 0.1 part of sepiolite, hydration sodium calcium aluminium silicate 0.1 based on sugared 10 parts, 8 parts of calcium formate, palygorskite mineral
Part, 0.5 part of copper sulphate, 5 parts of sodium salt and 25 parts of ratios of B B-complex weigh raw material, are well mixed, produce.
Embodiment 5
A kind of feed addictive containing compound marine alga, is made by the steps:
(1)It is prepared by brown alga carbohydrate:Using Kelp Powder as raw material, 12-18 times of water for adding its weight stirs, and boils 10-
15min, cool, after 28 DEG C are incubated 25-40min, add specific bacterial strain Pseudomonas. sp.HZJ216 productions
Algin catenase, stir evenly, homogenate is put into 28 DEG C of insulating boxs and digests 3h, the addition of its enzyme is homogenate weight
0.05-0.08%, in course of reaction otherwise stop stirring, boil enzymolysis reaction, got product after spraying drying;
(2)It is prepared by green alga carbohydrate:The enteromorpha powder washed, dry, crushed is pressed into solid-liquid ratio 1:35 add water, and 90-100 DEG C is extracted 4h,
It is cold go after, add green algae polysaccharide digestive enzyme in the solution, while add Mg2+In 40-50 DEG C, in pH value in the range of 7.5-8.5
0.5-2h is digested, after enzymolysis terminates, is heated to the 2-3min that seethes with excitement and be incubated, after cooling, adds sodium dihydrogen phosphate, adjustment pH is extremely
7.0 or so, spraying drying;
(3)It is prepared by vitamin:20 parts of 35 parts of vitamin A, 25 parts of vitamin E, 25 parts of vitamin C and yeast selenium are weighed, is carried out
It is well mixed, as B B-complex, it is standby;
(4)Crush:By the concave convex rod based on brown alga carbohydrate, green alga carbohydrate, yeast cell wall, modified montmorillonite used, palygorskite mineral,
Sepiolite and hydration sodium calcium aluminium silicate carry out ultramicro grinding, and powder is standby by 200 mesh sieves;
(5)Mixing:Gather respectively by 35 parts of brown alga carbohydrate, 25 parts of green alga carbohydrate, 5 parts of yeast cell wall, modified montmorillonite used 35 parts, shell
0.1 part of concave convex rod, 0.1 part of sepiolite, hydration sodium calcium aluminium silicate 0.1 based on sugared 6 parts, 6 parts of calcium formate, palygorskite mineral
Part, 0.5 part of copper sulphate, 5 parts of sodium salt and 30 parts of ratios of B B-complex weigh raw material, are well mixed, produce.
Embodiment 6
A kind of feed addictive containing compound marine alga, is made by the steps:
(1)It is prepared by brown alga carbohydrate:Using Kelp Powder as raw material, 12-18 times of water for adding its weight stirs, and boils 10-
15min, cool, after 28 DEG C are incubated 25-40min, add specific bacterial strain Pseudomonas. sp.HZJ216 productions
Algin catenase, stir evenly, homogenate is put into 28 DEG C of insulating boxs and digests 3h, the addition of its enzyme is homogenate weight
0.05-0.08%, in course of reaction otherwise stop stirring, boil enzymolysis reaction, got product after spraying drying;
(2)It is prepared by green alga carbohydrate:The enteromorpha powder washed, dry, crushed is pressed into solid-liquid ratio 1:35 add water, and 90-100 DEG C is extracted 4h,
It is cold go after, add green algae polysaccharide digestive enzyme in the solution, while add Mg2+In 40-50 DEG C, in pH value in the range of 7.5-8.5
0.5-2h is digested, after enzymolysis terminates, is heated to the 2-3min that seethes with excitement and be incubated, after cooling, adds sodium dihydrogen phosphate, adjustment pH is extremely
7.0 or so, spraying drying;
(3)It is prepared by vitamin:30 parts of 15 parts of vitamin A, 15 parts of vitamin E, 30 parts of vitamin C and yeast selenium are weighed, is carried out
It is well mixed, as B B-complex, it is standby;
(4)Crush:By the concave convex rod based on brown alga carbohydrate, green alga carbohydrate, yeast cell wall, modified montmorillonite used, palygorskite mineral,
Sepiolite and hydration sodium calcium aluminium silicate carry out ultramicro grinding, and powder is standby by 200 mesh sieves;
(5)Mixing:Gather respectively by 40 parts of brown alga carbohydrate, 35 parts of green alga carbohydrate, 5 parts of yeast cell wall, modified montmorillonite used 30 parts, shell
0.1 part of concave convex rod, 0.1 part of sepiolite, hydration sodium calcium aluminium silicate 0.1 based on sugared 5 parts, 10 parts of calcium formate, palygorskite mineral
Part, 0.5 part of copper sulphate, 5 parts of sodium salt and 25 parts of ratios of B B-complex weigh raw material, are well mixed, produce.
The embodiment of the present invention 1, embodiment 2, embodiment 3, embodiment 4, embodiment 5, the feed of embodiment 6 are carried out in laboratory
Additive is tested to the detoxification efficiency of mycotoxin.Experiment is divided into 8 groups, and the 7th group is deficiency test group, uses feed to add
It is by the brown alga carbohydrate equivalent substitution of the green alga carbohydrate in embodiment 6 to add agent;8th group of deficiency test group, uses feed
Additive is by the green alga carbohydrate equivalent substitution of the brown alga carbohydrate in embodiment 6.Mycotoxin is respectively zearalenone, Huang
Aspertoxin B1(AFB1), vomitoxin, T-2 toxin, fumonisin B1, take centrifuge tube add the above-mentioned mycotoxin standards of 10ml
Solution(It is shown in Table 1), then feed addictive sample 25mg of the present invention is added in centrifuge tube, makes feed addictive in the solution dense
Spend for 2.5mg/ml.All samples shake absorption 2h in 37 DEG C of thermostatic control oscillator vibrations with 150r/min.Then, with 3000r/
Min centrifuges 10min, collects supernatant, determines the content of various mycotoxins in each supernatant.Adsorbed in time according to calculation formula
Measure Q(ng/mg)With adsorption rate Y(%).Then 10ml pH7.0 PSB cushioning liquid is added in residue, in 37 DEG C, 150r/
Concussion parsing 30min in min thermostatic control oscillator vibrations, centrifuges 10min with 3000r/min, collects supernatant, determine again on each
The content of various mycotoxins in clear liquid.All samples set 2 repetitions.Accumulation parsing is obtained after blank group and control group correction
Rate(%).
Formula one:Q=V(C0-C1)/M
Formula two:Y=100*(C0-C1)/ C0
Formula three:R=C2/(C0-C1)*100%
Wherein, V is liquor capacity(ml);M is the dosage of feed addictive of the present invention(mg);C0For various mycotoxins before absorption
Concentration(ng/mg);C1For the concentration of various mycotoxins after absorption(ng/mg);C2For the concentration of various mycotoxins after desorption
(ng/mg).
Experiment measurement result is specifically shown in Table 1.
Detoxification experimental result of 1 feed addictive of the present invention of table to various mycotoxins
Test result indicates that blank control group is 0 to various mycotoxin adsorption rates, drug control group AFB1Adsorption rate exist
More than 90%, other are below 70%;Test group is above blank and control group to the adsorption rate of various mycotoxins, and adsorbs effect
Rate is example 6, example 5, example 4, example 3, example 2 and example 1 respectively from high to low, example 6 and example 5 with to each toxin
Adsorption efficiency is basically identical.Experiment shows that feed addictive of the present invention is to zearalenone, AFB1, vomitoxin, T-2 poison
Element, fumonisin B1 removal effect are all fine, and are tightly combined with toxin, and detoxification is more thorough, hence it is evident that are taken off better than modified cover
Removal effect of the stone to various mycotoxins.In addition, from the experimental results, the effect of the absorbing toxin of example 5 and example 6 is most
Alright.In addition, result of the test shows that the synergy between brown alga and green alga achieves " 1+1>2 " technique effect.
Claims (9)
1. a kind of feed addictive containing compound marine alga, it is characterised in that feed addictive is by brown alga carbohydrate, green alga carbohydrate, yeast
Cell membrane, modified montmorillonite used, chitosan, calcium formate, the concave convex rod based on palygorskite mineral, sepiolite, hydration sodium calcium manosil AS
Salt, copper sulphate, sodium salt and B B-complex composition, wherein the weight proportion of the raw material is as follows:Brown alga carbohydrate 1-40 parts, green alga
Carbohydrate 1-40 parts, yeast cell wall 1-40 parts, modified montmorillonite used 10-60 parts, chitosan 1-15 parts, calcium formate 1-40 parts, slope thread
Concave convex rod 0.1-40 parts, sepiolite 0.1-40 parts, hydration sodium calcium aluminium silicate 0.1-40 parts based on stone ore thing, copper sulphate
0.01-10 parts, sodium salt 0.01-10 parts and B B-complex 4-60 parts, the B B-complex are made up of following weight parts component:
Vitamin A 1-50 parts, vitamin E 1-50 parts, vitamin C 1-30 parts and yeast selenium 1-50 parts.
A kind of 2. feed addictive containing compound marine alga according to claim 1, it is characterised in that the feed addictive
Raw material proportioning be:Brown alga carbohydrate 10-30 parts, green alga carbohydrate 5-20 parts, yeast cell wall 1-25 parts, modified montmorillonite used 18-35
Part, chitosan 5-10 parts, calcium formate 5-30 parts, concave convex rod 0.1-30 parts based on palygorskite mineral, sepiolite 0.1-20 parts,
Sodium calcium aluminium silicate 0.1-20 parts, copper sulphate 0.01-5 parts, sodium salt 0.01-6 parts and B B-complex 5-50 parts are hydrated, it is described multiple
Vitamin is closed to be made up of following weight parts component:Vitamin A 5-40 parts, vitamin E 10-50 parts, vitamin C 5-30 parts and
Yeast selenium 15-50 parts.
A kind of 3. feed addictive containing compound marine alga according to claim 1, it is characterised in that the feed addictive
Raw material proportioning be:20 parts of brown alga carbohydrate, 16 parts of green alga carbohydrate, 10 parts of yeast cell wall, modified montmorillonite used 25 parts, chitosan 5
5 parts 1 part of concave convex rod, 5 parts of sepiolite, hydration of sodium calcium aluminium silicate based on part, 5 parts of calcium formate, palygorskite mineral, copper sulphate
1.5 parts, 5 parts of 1.5 parts of sodium salt and B B-complex, the B B-complex is made up of following weight parts component:Vitamin A 30
Part, 25 parts of vitamin E, 30 parts of vitamin C and 15 parts of yeast selenium.
A kind of 4. feed addictive containing compound marine alga according to claim 1-3, it is characterised in that the brown alga carbohydrate
It is made by the steps:Using brown alga powder as raw material, 12-18 times of water for adding its weight stirs, and boils 10-15min,
Cool, after 28 DEG C are incubated 25-40min, add the algin of specific bacterial strain Pseudomonas. sp.HZJ216 productions
Lyases, stir evenly, homogenate is put into 28 DEG C of insulating boxs and digests 3h, the addition of its enzyme is the 0.05- of homogenate weight
0.08%, in course of reaction otherwise stop stirring, boil enzymolysis reaction, got product after spraying drying.
A kind of 5. feed addictive containing compound marine alga according to claim 4, it is characterised in that the brown alga be sea-tangle,
One or more of mixtures in opotism, sargassum and bulk kelp.
A kind of 6. feed addictive containing compound marine alga according to claim 1-3, it is characterised in that the green alga carbohydrate
Prepare:The chlorella powder washed, dry, crushed is pressed into solid-liquid ratio 1:35 plus water, 90-100 DEG C of extraction 4h, it is cold go after, in the solution
Green algae polysaccharide digestive enzyme is added, while adds Mg2+In 40-50 DEG C, 0.5-2h is digested in the range of 7.5-8.5 in pH value, is digested
After end, the 2-3min that seethes with excitement and be incubated is heated to, after cooling, adds sodium dihydrogen phosphate, adjusts pH to 7.0 or so, spraying drying
.
A kind of 7. feed addictive containing compound marine alga according to claim 6, it is characterised in that the green alga be Enteromorpha,
One or more of mixtures in sea lettuce, reef film.
A kind of 8. feed addictive containing compound marine alga according to claim 1-3, it is characterised in that the feed addition
Agent preparation technology comprises the following steps:
(1)Weigh various vitamins respectively by weight ratio:Vitamin A, vitamin E, vitamin and yeast selenium, carry out uniformly,
As B B-complex;
(2)Respectively by the concave convex rod based on brown alga carbohydrate, green alga carbohydrate, yeast cell wall, modified montmorillonite used, palygorskite mineral,
Sepiolite and hydration sodium calcium aluminium silicate carry out ultramicro grinding, and powder weighs fucose respectively again by weight by 200 mesh sieves
Class, green alga carbohydrate, yeast cell wall, modified montmorillonite used, chitosan, calcium formate, the concave convex rod based on palygorskite mineral, Hai Pao
Stone, hydration sodium calcium aluminium silicate, copper sulphate, sodium salt and B B-complex are well mixed and produced.
A kind of 9. feed addictive containing compound marine alga according to claim 1-3, it is characterised in that the feed addition
Adding proportion of the agent in feed is 1-2kg/T.
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