The detoxicating mycotoxin agent of a kind of pig feed
Technical field
The present invention relates to the detoxicating mycotoxin agent of a kind of pig feed.Additive of the present invention can effectively be removed the harm of mycotoxin to growth of animal performance, production performance and health status, belongs to the feed additive field that ensures feed safety.
Background technology
Mycotoxin is the poisonous secondary metabolite that some mould produces in growth and breeding process on cereal or feed, has had up to now 300 kinds of mycotoxins of surpassing to be isolated and identified out.Herding in producing mycotoxin common and that can directly affect growth of animal production performance and health status mainly by four kinds of mould Pseudomonas, produced: Eurotium (mainly secreting aflatoxin, ochratoxin etc.), Penicillium (mainly secreting the mould toxin of tangerine), Claviceps (mainly secreting ergotoxin), fusobacterium (mainly secreting vomitoxin, zearalenone, T-2 toxin, fumonism etc.).Mycotoxin can cause that pig occurs that organa parenchymatosum damages (liver, kidney), immunosupress, (sow false heat or oestrous cycle extend breeding difficulty, after ablactation, insemination of sows returns the rising of feelings rate, Repeat breeding or occur pseudopregnancy, in-pig miscarriage or product stillborn foetus, milking sow lactation amount reduces or agalasisa, boar sexual desire is poor, degradation under sperm motility), feed intake decline, the stagnation of growing, become thin, the illness such as feed conversion rate reduction.
Cereal (feedstuff) thus field growing, results, storage, process and the process of feeding in all likely fungal infection cause the generation of mycotoxin.The harm of mycotoxins in feed has been serious problems of the global aquaculture of impact.According to FAO's investigation, the whole world is accounted for 25% left and right of its total amount every year by the cereal of mould contamination, oilseed and feed.More than 800 sample detection that 2006~2007 Nian, Ao Teqi companies gather Asia find, zearalenone, the deoxidation melon of raw food, feed etc. withers the positive rate of reaping hook alcohol, fumonism, aflatoxin all more than 90%.Ao Zhi just waits 225 sample detection is found; corn, complete feed, protein feed, corn byproducts (corn germ cake, corn protein powder, maize alcohol stillage etc.) milk cow mixed fodder; 6 kinds of mycotoxin recall rate average out tos 93.2% of ensilage; the highest vomitoxin reaches 99.1%; exceeding standard rate average out to 29.6%, the highest vomitoxin that exceeds standard reaches 53.5%.Within 2009, the Ministry of Agriculture is to coming from 32 corn samples in the whole nation, 46 protein raw materials samples, 23 complete feed samples detect discovery, aspergillus flavus toxin pollutes the most serious, zearalenone the second, T-2 toxin, vomitoxin, FT, ochratoxin pollute ubiquity.All samples all detects aspergillus flavus toxin and vomitoxin, 89% corn sample is zeranol positive reaction, and conventionally by three kinds or three kinds of above mycotoxin contaminations, 80% dregs of beans contains aspergillus flavus toxin, T-2 toxin and three kinds of toxin of zearalenone simultaneously.2011, Bai Aoming company has carried out the inferior detection of 13854 samples to 4327 samples from all over the world, and each mycotoxin positive rate is respectively: aflatoxin 27%, zearalenone 40%, vomitoxin 59%, FT 51%, ochratoxin 27%.
By rejecting mouldy or damaged seed, slough containing more outer covering of a kernel or the plumule of toxin, clear water is eluriated, the methods such as sunlight insolation, radiation, heating, can reduce the toxicity of mould quantity and reduction mycotoxin, but the method is difficult to thoroughly eliminate the toxin that mould produces.On market, there is in recent years multiple natural or synthetic mycotoxin scavenger, with removing mould and the mycotoxin having polluted in feed and raw material, by its mode of action, can be divided into following a few class: physics detoxicity method, chemical detoxication method, biological detoxication method etc. are several.
(1) physics detoxification: its principle is can absorbing mycotoxin material by adding in feed, and toxin is not absorbed by animals when through animal intestinal, directly excretes.Conventional mold toxin sorbent has, natural aluminosilicate (as zeolite, montmorillonite, diatomite, kaolin etc.), mannan-oligosaccharides etc.
Natural aluminosilicate to the adsorption capacity of mycotoxin not only with adsorbent with the distribution of total charge dosage, electric charge, the size of hole and available surface area (specific area) are relevant, and be decided by the physical features such as polarity, solubility, molecular size, molecular shape, distribution of charges of the lps molecule that is adsorbed.Natural aluminosilicate has advantages of larger specific area, but simultaneously also has certain defect: show as that absorption affinity is little and efficiency is low; Must heavy dose of (1-3%) add ability effectively in use, and only for aflatoxin and ergotin, have certain absorption affinity; To vomitoxin, T-2 toxin, zearalenone, ochratoxin A, snakelike verticillium toxin, Festuca Arundinacea toxin etc. does not have suction-operated; Nutriment in feed is also had to suction-operated, thereby reduce feed nutrition concentration; Use the feed of this kind adsorbent to exist by the risk of dioxin (dioxins) and heavy metal pollution.
Mannan-oligosaccharides prevent animal mycotoxin poisoning aspect,, effect few with its addition is significantly and cause people's extensive attention in conjunction with features such as mycotoxin kind are wide.Current large quantity research shows, mannan-oligosaccharides can with many mycotoxin combinations, eliminate the impact of mould on body.The simulation broiler chicken alimentary canal in vitro tests such as Raju show, mannan-oligosaccharides is respectively 82.5%, 51.6% and 26.4% to the combination rate of AFB1, FT toxin, ochratoxin.The in vitro test of Trenholm etc. shows, under pH=4.0 condition, mannan-oligosaccharides reaches respectively 80%, 95%, 77%, 59% and 12% to the knot platform rate of zearalenone, aflatoxin, zearalenone, fumonisin and vomitoxin.Mannan-oligosaccharides is the oligosaccharide kind material mainly extracting from yeast cell wall skin, and therefore obtaining mannan-oligosaccharides needs tightly high fractionation technique accurately.Domestic shortage is by yeast cell wall demixing technology, just simply yeast cell wall integral body is pulverized and applied as immunostimulant and mould adsorbant function product, cause mannan-oligosaccharides active ingredient dosage on the low side and absorbing mycotoxin effect is differed greatly with the result of bibliographical information, limited greatly promoting the use of in market.
(2) chemical detoxication.Some mycotoxin can be destroyed degraded by the chemicals of part, as common, with the mouldy seed of soda lye wash, with ammoniacal liquor or copper-bath, soaks moldy feed.But there is following drawback in the method: different chemical substances often only effectively can not be degraded to other toxin to certain toxin, and the chemical substance of use is residual and produce new poisonous and harmful effect in feed.(3) biological detoxication.Mainly contain enzymatic isolation method and microbe fermentation method, enzymatic isolation method is by enzyme digestion reaction, to make functional group/toxicity inactivation of mycotoxin.The limiting factor that the method is subject to has, and in essence, enzyme is also protein, and its vigor can be subject to pH, temperature and be subject to the impact of matter (mycotoxin).The defect of the method is: feed may not necessarily provide the environment that is applicable to this kind of enzyme; Enzyme, to being subject to the narrow spectrum degree of selection of matter very high, be critical sometimes, so these class methods equally only has certain effect to certain toxin; Secondary species after enzymolysis is likely toxic; Enzyme is thermo-labile, at the easy inactivation of feed process.The effect of microbe fermentation method is slow, is not suitable for feed factory and plant.Therefore, use that biological detoxication method cost is higher and effect is unstable, thus in production practices still difficulty apply.
(4) normal flora and Chinese herbal medicine detoxicity method.In recent years, having and in feed, add the report that removes aflatoxin for lactic acid bacteria, is mainly by keeping gut flora balance, strengthens the immunity of body to alleviate the harm of toxin.The method contratoxin does not have specificity, only rests on the experimental stage, from practical application, differs greatly.
Since 2007, detoxicating mycotoxin agent commodity all emerge in an endless stream every year, but annual feed mould Positive rate, the poisoning incidence of disease of livestock and poultry mycotoxin remain high, and from the angle of actual production practice, existing detoxicating mycotoxin agent effect on market is undesirable.
The drawback occurring for single detoxicating mycotoxin agent, the catalogue > > of the < < integration of drinking and medicinal herbs that the inventor announces according to the Ministry of Public Health, at the material that is food and medicine, can be used for filtering out the strong natural plants of several antifungi effects in the material of health food, and then with the composite composite mold toxin detoxifying agent of making of organic modification montmorillonite, from the angle that mould fungus inhibition is bred and absorbing mycotoxin is worked along both lines, reach the object of removing Mycotoxins in Feed.
Summary of the invention
The present invention is the floristic regulation about integration of drinking and medicinal herbs according to health ministry, filter out the strong natural organic plants of antifungi effect such as Chinese cassia tree, cloves, Chinese prickly ash, galangal, dark plum, Ligusticum wallichii, turmeric, purslane, dandelion, by reasonable processing and prescription, and then with the montmorillonite reasonable formula after organic modification, final purpose be to provide a kind of can mould fungus inhibition breeding, composite mold toxin detoxifying agent that again can absorbing mycotoxin.
Technical scheme of the present invention be with dandelion extract, Flos Caryophylli extract, galangal rhizome extract, purslane herb Ultramicro-powder mince, Chinese prickly ash Ultramicro-powder minces, Chinese cassia tree Ultramicro-powder minces,, dark plum micro mist minces, Ligusticum wallichii Ultramicro-powder minces, turmeric Ultramicro-powder minces into Main Function thing, by suppressing or killing mould common in feed and play the effect of eradicating mycotoxin source from source; The montmorillonite of organic modification of take is secondary role thing, adsorbs the effect of playing mycotoxin in adsorption feed by physical property.This compound composite mold toxin detoxifying agent makes an addition in swine feed, can reach aflatoxin, FT, vomitoxin, zearalenone, the effective scavenging action of T-2 toxin.
The invention provides a kind of can mould fungus inhibition breeding, composite mold toxin detoxifying agent that again can absorbing mycotoxin, it is by various plants micro mist and plant extracts, a kind of organic modification montmorillonite proportioning forms.
The invention provides a kind of composite mold toxin detoxifying agent, it forms (each amounts of components is weight portion) by following component
The montmorillonite 35~45 of organic modification
Dandelion extract 5~10
Flos Caryophylli extract 5~10
Galangal rhizome extract 5~15
Purslane herb Ultramicro-powder minces 15~20
Chinese prickly ash Ultramicro-powder minces 10~15
Chinese cassia tree Ultramicro-powder minces 20~30
Ligusticum wallichii Ultramicro-powder minces 15~25
Turmeric Ultramicro-powder minces 20~25
In a preferred embodiment of the invention, the invention provides a kind of consist of (each amounts of components is weight portion) of composite mold toxin detoxifying agent:
The montmorillonite 40 of organic modification
Dandelion extract 10
Flos Caryophylli extract 10
Galangal rhizome extract 10
Purslane herb Ultramicro-powder minces 20
Chinese prickly ash Ultramicro-powder minces 10
Chinese cassia tree Ultramicro-powder minces 20
Ligusticum wallichii Ultramicro-powder minces 15
Turmeric Ultramicro-powder minces 20
Detoxification experimental results show that: organic composite type detoxicating mycotoxin of the present invention agent is added by 0.1~0.4 kilogram/100 kilograms batches, can reduce the poisoning symptom of degradation mycotoxin under swinery false heat, watery stools, expiratory dyspnea, food refusal or feed intake.
Therefore, the invention provides composite mold toxin detoxifying agent of the present invention improving organa parenchymatosum damage (liver, kidney), immunosupress, breeding difficulty, feed intake decline, the stagnation of growing, become thin, the purposes aspect the illness such as feed conversion rate reduction.
In a preferred embodiment of the invention, the pig that described animal is each age level.Addition in feed is 0.15 kilogram/100 kilograms complete diet pellets.
On the other hand, the invention provides the preparation method of a kind of organic composite type detoxicating mycotoxin agent.The method comprises the following steps:
(1) extraction of montmorillonite and organic modification
Purify and accurately to take 100g days bentonites and 4g sodium carbonate, be placed in 2000ml beaker, add 900ml distilled water and 3~l0ml, 10% sodium hexametaphosphate solution dispersant, make 10% suspension; Proceed in high speed dispersor, 3000r/min constantly stirs 1h; Be placed in the centrifugal l~10min of centrifuge 3000r/min, remove the thicker sediment in bottom; Be diluted with water l and be doubly placed in high-speed homogenization machine and disperse 30~60min, centrifugal 1~5min, removes top particulate and clear liquid, then centrifugal dehydration 30~60min, gets dry 24h at l05 ℃ of bottom precipitation thing, and grinding is sieved, and screenings is standby, makes na-montmorillonite.
In 500ml flask, add a certain amount of na-montmorillonite, softex kw, appropriate distilled water, and dripping a small amount of dilute sulfuric acid, to adjust pH value be 3, be put in and in 60X2 water bath with thermostatic control, heat stir about 2h, it is fully exchanged, will be after the montmorillonite slip centrifugation at normal temperatures after intercalation with distilled water washing more than 3 times, can't detect Br-to eluate; Drying under reduced pressure at 40 ℃, 105 ℃ of activation 30min, are crushed to 200 orders with polishing, are placed in drier and preserve, and make organically modified montmorillonite clay.
(2) preparation of purslane micro mist: get purslane herb, impurity elimination, grind into powder.Collect by the micro mist of 100 mesh sieve sheets.
(3) preparation that Chinese prickly ash Ultramicro-powder minces: get Chinese prickly ash dry mature fruit, impurity elimination, grind into powder.Collect by the micro mist of 100 mesh sieve sheets.
(4) preparation that Chinese cassia tree Ultramicro-powder minces: get the dry bark of Chinese cassia tree, impurity elimination, grind into powder.Collect by the powder of 100 mesh sieve sheets.
(5) preparation that Ligusticum wallichii Ultramicro-powder minces: get the dry rhizome of Ligusticum wallichii, impurity elimination, grind into powder.Collect by the micro mist of 100 mesh sieve sheets.
(6) preparation that turmeric Ultramicro-powder minces: get the dry rhizome of Ligusticum wallichii, impurity elimination, grind into powder.Collect by the micro mist of 100 mesh sieve sheets.
(7) dandelion herb extract: commercial, require general flavone content to be greater than 5%.
(8) Flos Caryophylli extract: commercial, require containing volatile oil 16~19%.
(9) galangal rhizome extract: commercial, require extract powder, 10:1.
(10) material above-mentioned steps being obtained by weight ratio mixes.
The montmorillonite 35~45 of organic modification
Dandelion extract 5~10
Flos Caryophylli extract 5~10
Galangal rhizome extract 5~15
Purslane herb Ultramicro-powder minces 15~20
Chinese prickly ash Ultramicro-powder minces 10~15
Chinese cassia tree Ultramicro-powder minces 20~30
Ligusticum wallichii Ultramicro-powder minces 15~25
Turmeric Ultramicro-powder minces 20~25
In a preferred embodiment of the invention, wherein the ratio by weight described in step (10) is mixed into:
The montmorillonite 40 of organic modification
Dandelion extract 10
Flos Caryophylli extract 10
Galangal rhizome extract 10
Purslane herb Ultramicro-powder minces 20
Chinese prickly ash Ultramicro-powder minces 10
Chinese cassia tree Ultramicro-powder minces 20
Ligusticum wallichii Ultramicro-powder minces 15
Turmeric Ultramicro-powder minces 20
advantage of the present invention
Composite mold toxin detoxifying agent of the present invention is the floristics of selecting the integration of drinking and medicinal herbs that the Ministry of Public Health issues, through Crushing of Ultrafine or extraction, the processes such as concentrated, form, have that raw material sources are extensive, bioavailability is high, the feature of nontoxic, noresidue, the organic modification of montmorillonite has improved adsorption capacity and has widened absorption spectrum.These organic substances composite, provide a kind of can mould fungus inhibition breeding from source, composite mold toxin detoxifying agent that again can absorbing mycotoxin, has improved pig growth performance, production performance, health status.
Below in conjunction with embodiment, the specific embodiment of the present invention is described further.Following examples are only for the present invention is described, but are not used for limiting the scope of the invention.
Embodiment
preparation and the organic modification of embodiment 1 montmorillonite
Purify and accurately to take 100g days bentonites and 4g sodium carbonate, be placed in 2000ml beaker, add 900ml distilled water and 3~l0ml, 10% sodium hexametaphosphate solution dispersant, make 10% suspension; Proceed in high speed dispersor, 3000r/min constantly stirs 1h; Be placed in the centrifugal l~10min of centrifuge 3000r/min, remove the thicker sediment in bottom; Be diluted with water l and be doubly placed in high-speed homogenization machine and disperse 30~60min, centrifugal 1~5min, removes top particulate and clear liquid, then centrifugal dehydration 30~60min, gets dry 24h at l05 ℃ of bottom precipitation thing, and grinding is sieved, and screenings is standby, makes na-montmorillonite.
In 500ml flask, add a certain amount of na-montmorillonite, softex kw, appropriate distilled water, and dripping a small amount of dilute sulfuric acid, to adjust pH value be 3, be put in and in 60X2 water bath with thermostatic control, heat stir about 2h, it is fully exchanged, will be after the montmorillonite slip centrifugation at normal temperatures after intercalation with distilled water washing more than 3 times, can't detect Br-to eluate; Drying under reduced pressure at 40 ℃, 105 ℃ of activation 30min, are crushed to 200 orders with polishing, are placed in drier and preserve, and make organically modified montmorillonite clay.
the preparation that embodiment 2 Plant Powders mince
The preparation of purslane micro mist: get purslane herb, impurity elimination, grind into powder.Collect by the micro mist of 100 mesh sieve sheets.
The preparation that Chinese prickly ash Ultramicro-powder minces: get Chinese prickly ash dry mature fruit, impurity elimination, grind into powder.Collect by the micro mist of 100 mesh sieve sheets.
The preparation that Chinese cassia tree Ultramicro-powder minces: get the dry bark of Chinese cassia tree, impurity elimination, grind into powder.Collect by the powder of 100 mesh sieve sheets.
The preparation that Ligusticum wallichii Ultramicro-powder minces: get the dry rhizome of Ligusticum wallichii, impurity elimination, grind into powder.Collect by the micro mist of 100 mesh sieve sheets.
The preparation that turmeric Ultramicro-powder minces: get the dry rhizome of turmeric, impurity elimination, grind into powder.Collect by the micro mist of 100 mesh sieve sheets.
acquisition and the standard of embodiment 3 plant extracts
Dandelion herb extract: be purchased from Xi'an grass roots bioengineering Co., Ltd, general flavone content is greater than 5%.
Flos Caryophylli extract: be purchased from the three-dimensional Bioisystech Co., Ltd in Xi'an, containing volatile oil 16~19%.
Galangal rhizome extract: be purchased from Rui Yuan bio tech ltd, Xi'an, 10:1 extract.
embodiment 4 composite mold toxin detoxifying agent preparations
The plant plant extracts of the montmorillonite of the organic modification of embodiment 1 preparation and embodiment 3 acquisitions is pressed to following weight portion proportioning:
The montmorillonite 40 of organic modification
Dandelion extract 10
Flos Caryophylli extract 10
Galangal rhizome extract 10
Above-mentioned each component fully mixes, and obtains mixture A.
The plant micro mist of embodiment 2 preparations is pressed to following weight portion proportioning:
Purslane herb Ultramicro-powder minces 20
Chinese prickly ash Ultramicro-powder minces 10
Chinese cassia tree Ultramicro-powder minces 20
Ligusticum wallichii Ultramicro-powder minces 15
Turmeric Ultramicro-powder minces 20
Each component is fully mixed, obtain mixture B
Mixture A and mixture B are fully mixed, obtain this composite mold toxin detoxifying agent
the adsorbing extracorporeal simulating experiment of embodiment 5 composite mold toxin detoxifying agent
1. experimentation
(1) get 3 sizes, internal diameter, the highly consistent transparent water tumbler of white, be filled with water to 4/5 place; Be numbered respectively 1,2,3
(2) with suction pipe, to being numbered, in 1 water tumbler, drip three Parker inks (simulation mycotoxin).
(3) get two vitamin B2s (people's use, represents nutriment), fully after grinding, put into the cup that is numbered 2, stir and make aqueous solution yellowly.
(4) No. 3 cup does not add anything cup in contrast.
(5) to the composite mold toxin detoxifying agent that adds respectively 2g to detect in three cups.
(6) each beaker stirs 5 minutes with glass bar, after standing 40 minutes, sees result.2. experimental result and conclusion
It is colourless that No. 1 water tumbler solvent presents, and a cup end solute is black, and No. 2 cup remains yellow, and No. 3 cup nondiscolourings, illustrate that this composite mold toxin detoxifying agent does not adsorb nutrition in absorbing mycotoxin.
the inhibitory action of embodiment 6 composite mold toxin detoxifying agents to mould
With aspergillus flavus, for representing mould, observe the inhibitory action of composite mold toxin detoxifying agent of the present invention to mould.
(1) on Czapek's medium (CA) basis, add composite mold toxin detoxifying agent of the present invention.
Concrete steps are: take sodium nitrate 3g, dipotassium hydrogen phosphate 1g, magnesium sulfate (MgSO47H2O) 0.5g, potassium chloride 0.5g, ferrous sulfate 0.01g, sucrose 30g, agar 20g, 2 grams of composite mold toxin detoxifying agents, distilled water 1000mL, heating for dissolving is poured the mycotoxin liquid 2ml of new preparation into when being cooled to 60 ℃ of left and right, then pours mixed liquor into glass culture dish.
(2) in 25~28 ℃ of cultivations, observed result after 3-5 days.
Result and conclusion
Do not see and have fungus growth, illustrate that organic composite type detoxicating mycotoxin of the present invention agent has good mould fungus inhibition effect.
embodiment 7 organic composite type detoxicating mycotoxin agent are measured mycotoxin adsorption ability
This experiment is gastrointestinal tract environment in analogue body, the absorption situation of this organic composite type detoxicating mycotoxin agent to AFB1, zearalenone, vomitoxin, FT and T-2 toxin that adopted two-phase method system evaluation.
1. the preparation of mycotoxin titer and adsorption test
The preparation of (1) 0.1 mol/L PBS (pH 3.0 and pH 6.8)
Precision takes in the distilled water of 35.814 g disodium hydrogen phosphate dodecahydrate to 900 mL, then uses phosphorus acid for adjusting pH value to 3.0 and 6.8, standby after constant volume.
(2) standard mycotoxin working solution (pH 3.0)
According to Mycotoxins in Feed country limit standard, and the 2-3 that pig amount of drinking water is feed intake doubly.The content of determining mycotoxin in working solution is as follows respectively:
AFB1 is 150ppb, and zearalenone is 50ppb, and vomitoxin is 5ppm, and T-2 toxin is 0.5ppm, and FT is 50ppb.
(3) adsorption test in adsorption test 1(simulation stomach, PH=3.0)
Take 0.75mg mold toxin sorbent sample in test tube, add l L pH3.0 mycotoxin working solution, in system, the addition of mold toxin sorbent is 0.75 mg/L.Blank is not for adding the 1L pH3.0 mycotoxin working solution of any mold toxin sorbent.Get above-mentioned working solution 10ml, put into respectively a test tube, test tube is placed on 37 ℃ of constant temperature oscillators, shake 1 h.After reaction finishes, in centrifugal 10 min of 3 500 r/m, ELISA method is measured the mycotoxin content in supernatant.
(4) adsorption test in adsorption test 2(simulation small intestine, PH=6.8)
After above-mentioned experiment finishes, supernatant in the centrifuge tube that inclines adds 10 mL pH6.8 phosphate buffers in residue, 37 ℃ of isothermal vibration 1 h.After reaction finishes, in centrifugal 10 min of 3 500 r/m, ELISA method is measured remaining mycotoxin content in supernatant.
(5), according to the content that detects toxin, by following formula, calculate the adsorption efficiency of mold toxin sorbent.
Y=100×(A-B-C)/A
In above formula: Y represents the adsorption efficiency of mold toxin sorbent to mycotoxin; A represents blank group (being not add adsorbent in mycotoxin working solution) content; B represents in adsorption test 1 content of mycotoxin in supernatant; C represents in desorption test 2 content of mycotoxin in supernatant.
2. result of the test and conclusion
The agent of this organic composite type detoxicating mycotoxin is to aflatoxin, zearalenone, vomitoxin, and the adsorption rate of T-2 toxin, FT is respectively 92.3%, 86.7%, 85.4%, 72.3%.78.4%, illustrate that composite mold toxin detoxifying agent of the present invention can effectively adsorb above-mentioned mycotoxin.
embodiment 8 detoxification experiments
The preparation of test daily ration and experimental animal
(1) preparation corn-soybean meal complete feed, corn-soybean meal is without mildew phenomena, as daily ration 1
(2) preparation Corn-soybean-peanut meal type complete feed, corn, dregs of beans, peanut meal have obvious mildew phenomena, as daily ration 2.
(3) get part daily ration 2, add wherein composite mold toxin detoxifying agent of the present invention, dosage is 0.15 kilogram/100 kilograms complete feeds, as daily ration 3.
Every nutritive index of above-mentioned three daily rations is identical.
(4) get 60 sizes and the identical pig of genetic background, be equally divided into three groups, 20 every group, to compile respectively as tri-groups of A, B, C, the weight average of A group is 60.5 kilograms, and the average weight of B group is 61.8 kilograms, and the average weight of C group is 61.5 kilograms.The A group daily ration 1 of feeding, the B group daily ration 2 of feeding, the C group daily ration 3 of feeding, feed period 20 days.
2. result of the test and conclusion
It is 78.8 kilograms that A group feed period finishes rear average weight, and any symptom does not appear in swinery; B group feed and finish after average weight be 78.5 kilograms, have two pig to occur diarrhoea phenomenon; C group feed and finish after average weight be 70.3 kilograms, the pig uniformity is poor, there is severe diarrhea phenomenon in 65% pig, ight soil is water sample, spurting; There is severe dyspnea in 45% pig, 35% pig occurs that vulva is red and swollen, and all pig occurs that feed intake obviously reduces by the in disorder phenomenon of hair.
Result of the test explanation detoxifying agent of the present invention is absorbing mycotoxin effectively, reduces immunosupress, false heat, feed intake that mycotoxin brings slow, the clinical symptoms such as become thin that decline, grow.