CN104068217B - A kind of ox antifungi toxin feed addictive - Google Patents
A kind of ox antifungi toxin feed addictive Download PDFInfo
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- CN104068217B CN104068217B CN201410259666.9A CN201410259666A CN104068217B CN 104068217 B CN104068217 B CN 104068217B CN 201410259666 A CN201410259666 A CN 201410259666A CN 104068217 B CN104068217 B CN 104068217B
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Abstract
The invention discloses a kind of ox antifungi toxin feed addictive, it is made up of the raw material of following parts by weight: the root of large-flowered skullcap 10 ~ 14 parts, cloves 8 ~ 12 parts, peppermint 8 ~ 12 parts, 12 ~ 18 parts, the leaf of bamboo, Tea Polyphenols 1 ~ 5 part, sodium Diacetate 3 ~ 8 parts, nano montmorillonite 30 ~ 50 parts, active saccharomyces cerevisiae 8 ~ 25 parts.The present invention can effectively absorbing mycotoxin, prevent ox feed mold, effectively promote that body resists the absorption to mycotoxin, strengthen body immunity, regulate beef cattle gastrointestinal function function, promote the absorption of nutriment, there is effect of stomach strengthening and digestion promoting, penetration enhancement growth.
Description
Technical field
The present invention a kind of feed addictive, particularly a kind of ox antifungi toxin feed addictive.
Background technology
Mycotoxin is the toxic metabolic products that crops or animal protein produce because of mould, and it has been found that hundreds of mycotoxin at present, these toxin have different toxicity, metabolic pathway and target organ in animal body.The harm that mycotoxin causes is a global problem, is present in nearly all feedstuff and human food's raw material.The pollution of Mycotoxins in Feed and the harm caused thereof are still the problem that culturist is easy to ignore at present, and easily and Other diseases produce and obscure.Estimate according to FAO (Food and Agriculture Organization of the United Nation), the whole world every year nearly 5% ~ 7% the agricultural product such as grain, feed suffer the erosion of mould.
Mould is a kind of many cells microorganism, and its procreation lower generation is by seed or the form of embracing son.Mould lake is prevalent in soil and some rotted plant materials.Mould in soil embraces son via air, water and entomochory on plant, once lake touches the seed broken, armful son is front rapidly to be sent out, and just can obviously see mouldy phenomenon.The procreation of these moulds is got up can produce more son of embracing and is gone to infect other seed.In field, plant is a lot of by the factor of mycotic infection, comprising: the moisture of soil, sowing time, harvest time.The kind of crop rotation phase, fertilising, plant, plant the generation of disease.Weeds, birds and insect etc.When after crop harvesting, usually can with some mould, in the process of drying, mould can be damaged, so not easily discover, but many mould lakes can survive, and start germination and growth in the process of lay up period, Fodder making.
Mycotoxin mainly produced by 4 kinds of mould Pseudomonas: Eurotium (Major Secretory aflatoxin, ochratoxin etc.), Penicillium (Major Secretory citrinin etc.).Claviceps (Major Secretory ergotoxin).Fusobacterium (Major Secretory vomitoxin, zearalenone, Fumonisin toxin etc.).Had up to now and be isolated and identified out more than 300 kinds of mycotoxins, above-mentioned several toxin are the 8 kinds of primary toxins be generally familiar with now.
Generally mould is divided into warehousing mould and two kinds, field mould by its life habit.Warehousing mould mainly refers to feed or the raw material of storage, and the mould produced under the suitable condition such as temperature, humidity, based on Eurotium, such mould optimum growth temperature is 25-30 DEG C, and relative humidity is 80%-90%, and road aspergillus makes an exception, can infect in field, also can breed under low temperature; Field mould then refers to Penicillium, Claviceps and Fusarium (Fusarium), this type of mould Pseudomonas field bacterial strain, usual cereal just infects before not gathering, optimum growth temperature is 5-25 DEG C, such mould also can breed in low temperature environment, that is this type of mould still can grow in the winter time, and the weather of cool, damp is easier to these fungus growths.
Mycotoxin is the poisonous secondary metabolic product that some mould produces in growth and breeding process on cereal or feed, and toxin all can produce in the manufacture of the production process of cereal, feed, storage and transportation.What cause very large harm to livestock and poultry is the mycotoxin produced by mould.
The method solving mould and mycotoxin has a variety of, and conventional method has: radiation sterilization, interpolation mould inhibitor, and breaks through on mouldproof packaging, material.And the main method of adding mould inhibitor has: 1, physisorphtion, as montmorillonite is processed, mould and mycotoxin can be adsorbed in mass efficient ground, the adsorption capacity of physical absorbent usually with adsorbent relevant with the distribution of total charge dosage, electric charge, the size of hole and available surface area (specific area), and be decided by by physical features such as the polarity of the lps molecule adsorbed, solubility, molecular size, molecular shape, distribution of charges; Therefore there is certain defect in physical absorbent: show as that absorption affinity is limited, efficiency is low, can not guarantee all have adsorption effect to all moulds.2, chemical ablation method, as added propionic acid or sorbic acid salt, can destroy mould function, hinders mould metabolism, reduces the toxic and side effect of the multiple moulds such as aspergillus flavus, Aspergillus ochraceus, aspergillus fumigatus, zeranol; Different chemical substances often only effectively can not be degraded to other toxin to certain toxin, and the chemical substance of use remains and produces new poisonous and harmful effect in feed.3, biological degradation method, mainly contains enzymatic isolation method and microbe fermentation method, but the biological detoxication method cost used at present is higher and effect unstable, thus in production practices still difficulty apply, etc.
At present, on market, to take off mould dose of kind a lot of for feed, are mostly clay class adsorbent, and it is to the adsorption capacity of mycotoxin difference distinguishing to some extent because of clay kind and source.This type of clay class adsorbent is owing to having multiple stratiform space structure, while absorbing mycotoxin, also nutriment can be adsorbed, and it is adsorbing selective stronger, different types of clay only has suction-operated to several or single mycotoxin, so in use there is some defects and drawback.
Summary of the invention
In order to solve above-mentioned Problems existing, the present invention has prepared a kind of ox feed addictive with antifungi and toxin function thereof, can effectively, in quick solution and alleviation crop and feed because of negative effect that mould and mycotoxin bring.
The object of the invention is to a kind of ox antifungi toxin feed addictive.
The technical solution used in the present invention is:
A kind of ox antifungi toxin feed addictive, it is made up of the raw material of following parts by weight: the root of large-flowered skullcap 10 ~ 14 parts, cloves 8 ~ 12 parts, peppermint 8 ~ 12 parts, 12 ~ 18 parts, the leaf of bamboo, Tea Polyphenols 1 ~ 5 part, sodium Diacetate 3 ~ 8 parts, nano montmorillonite 30 ~ 50 parts, active saccharomyces cerevisiae 8 ~ 25 parts.
Further, a kind of ox antifungi toxin feed addictive, it is made up of the raw material of following weight portion array: the root of large-flowered skullcap 12 parts, cloves 10 parts, peppermint 10 parts, 15 parts, the leaf of bamboo, Tea Polyphenols 1.5 parts, sodium Diacetate 5.5 parts, nano montmorillonite 45 parts, active saccharomyces cerevisiae 20 parts.
Further, the viable bacteria concentration of above-mentioned active saccharomyces cerevisiae is no less than 2 × 10
10individual/gram.
The invention has the beneficial effects as follows:
1) additive of the present invention is based on Chinese herbal and crude drugs preparations, and be aided with the necessary salinity of animal body and useful active saccharomyces cerevisiae preparation, Long-Time Service drug residue free and toxic and side effect, meet the requirement of national green food production.Especially can effectively absorb and remove the harm that mould and mycotoxin bring to animal, improve beef cattle feed intake, promote growth of animal, improve animal immune ability, reduce disease and occur, especially to animal alimentary canal diseases, there is obvious prevention and corntrol effect.
2) additive of the present invention can effectively absorbing mycotoxin, prevent ox feed mold, effectively promote that body resists the absorption to mycotoxin.
3) additive raw material of the present invention can obtain in a large number, suitable mass industrialized production.
Detailed description of the invention
The ox of the present invention action principle of antifungi toxin feed addictive:
Although prescription component of the present invention is few, but forgive material resources absorption principle, chemical ablation principle, biodegradation principle, and the traditional Chinese medical science is nursed one's health and Principles, make these four kinds to act on and remove in mould and mycotoxin mechanism the effect playing collaborative promotion, make mould and mycotoxin be controlled effectively to links in body from external and remove, and promote the reparation to animal and health-care effect.Montmorillonite described in the present invention's formula, having another name called montmorillonite, is a kind of alumino-silicate, has very strong suction-operated, especially nano montmorillonite, is the first-selection of mould adsorbent, and is a kind of safe green product; A kind of edible chemicals of sodium Diacetate, the fungusproof anti-corrosion agent of feed and food, has the effect increasing feed intake and weightening finish; Tea Polyphenols has very strong antioxidation, can be used for food fresh keeping anticorrosive mildewproof, has no side effect, edible safety; Active saccharomyces cerevisiae can increase profitable strain quantity in livestock and poultry body, reduces harmful mould living space, by its toxicity of enzyme preparation degrades; Baikal skullcap root has heat-clearing and damp-drying drug, purging intense heat and detonicating, hemostasis, antiabortive, hypotensive, and for damp-warm syndrome, heat temperature evil of vomitting uncomfortable in chest, damp and hot ruffian is full, rushes down dysentery, jaundice etc.; Cloves has in temperature, warms up kidney, falls inverse effect, all has curative effect to hiccup, vomiting, gastric disorder causing nausea, dysentery, ringworm etc.; Peppermint has dispelling wind, and heat radiation, wards off dirty, detoxicating functions; The leaf of bamboo is very common in south, has effect that is clearing heat and detoxicating, anti-inflammation.The described root of large-flowered skullcap, cloves, peppermint, the leaf of bamboo, Tea Polyphenols, sodium Diacetate, nano montmorillonite, active saccharomyces cerevisiae meet the relevant regulations in " Chinese veterinary pharmacopoeia ".The Bile duct injury that above-mentioned Chinese traditional medicine composition can cause mycotoxin, liver renal toxicity play protection and physiotherapy function, can protect the liver and protect courage, nest is protected in enriching yin, promote immunity, repair the impaired internal organs of animal, reply organ physiological function.
Below in conjunction with specific embodiment, the present invention is further illustrated, but be not limited thereto.
embodiment 1:
A kind of beef cattle anti-heat stress feed additive, takes the root of large-flowered skullcap 12 parts, cloves 10 parts, peppermint 10 parts, 15 parts, the leaf of bamboo, Tea Polyphenols 1.5 parts, sodium Diacetate 5.5 parts, nano montmorillonite 35 parts, active saccharomyces cerevisiae 10 parts by weight.Ultramicro grinding is carried out in the root of large-flowered skullcap, cloves, peppermint, leaf of bamboo mixing, crosses 60 order shaking screens, then stir with Tea Polyphenols, sodium Diacetate, nano montmorillonite, 60 DEG C of oven dry, sterilizing, cooling, then mix with active saccharomyces cerevisiae, pack.
embodiment 2:
A kind of beef cattle anti-heat stress feed additive, takes the root of large-flowered skullcap 12 parts, cloves 10 parts, peppermint 10 parts, 15 parts, the leaf of bamboo, Tea Polyphenols 1.5 parts, sodium Diacetate 5.5 parts, nano montmorillonite 45 parts, active saccharomyces cerevisiae 20 parts by weight.Ultramicro grinding is carried out in the root of large-flowered skullcap, cloves, peppermint, leaf of bamboo mixing, crosses 60 order shaking screens, then stir with Tea Polyphenols, sodium Diacetate, nano montmorillonite, 60 DEG C of oven dry, sterilizing, cooling, then mix with active saccharomyces cerevisiae, pack.
embodiment 3:
A kind of beef cattle anti-heat stress feed additive, takes the root of large-flowered skullcap 10 parts, cloves 8 parts, peppermint 12 parts, 18 parts, the leaf of bamboo, Tea Polyphenols 5 parts, sodium Diacetate 8 parts, nano montmorillonite 30 parts, active saccharomyces cerevisiae 25 parts by weight.Ultramicro grinding is carried out in the root of large-flowered skullcap, cloves, peppermint, leaf of bamboo mixing, crosses 60 order shaking screens, then stir with Tea Polyphenols, sodium Diacetate, nano montmorillonite, 60 DEG C of oven dry, sterilizing, cooling, then mix with active saccharomyces cerevisiae, pack.
embodiment 4:
A kind of beef cattle anti-heat stress feed additive, takes the root of large-flowered skullcap 14 parts, cloves 12 parts, peppermint 8 parts, 12 parts, the leaf of bamboo, Tea Polyphenols 1 part, sodium Diacetate 3 parts, nano montmorillonite 50 parts, active saccharomyces cerevisiae 8 parts by weight.Ultramicro grinding is carried out in the root of large-flowered skullcap, cloves, peppermint, leaf of bamboo mixing, crosses 60 order shaking screens, then stir with Tea Polyphenols, sodium Diacetate, nano montmorillonite, 60 DEG C of oven dry, sterilizing, cooling, then mix with active saccharomyces cerevisiae, pack.
Further effect detection can be made with anti-heat stress feed additive below to beef cattle prepared in embodiment.
one, antifungi toxin feed addictive of the present invention is to the detoxification efficiency of multiple mycotoxin
Carry out the embodiment of the present invention 2 antifungi toxin feed addictive to test the detoxification efficiency of mycotoxin in laboratory.Prepare zearalenone (2 μ g/mL) respectively, vomitoxin (5 μ g/mL), AFB1 (AFB1) (0.1 μ g/mL), FT be (0.05 μ g/mL), fumonisin B1(1 μ g/mL) standard liquid, get centrifuge tube and add 10mg antifungi toxin of the present invention feed addictive, add more than 10ml mycotoxin standard liquid again, make antifungi toxin feed addictive concentration in the solution be 2.5mg/ml(embodiment 2 groups).Arrange blank group (not adding detoxifying agent) and comparative example group (adding modified montmorillonite used), all samples establishes 3 repetitions simultaneously.All samples adsorbs 2h to vibrate in 37 DEG C of water bath with thermostatic control oscillators.Then, with collected by centrifugation supernatant, measure the content of each mycotoxin in supernatant and calculate adsorption rate Y (%)=100 × (C of each mycotoxin
0-C
1)/C
0, C
0for adsorbing the concentration of front each mycotoxin; C
1for adsorbing the concentration of rear each mycotoxin.
By above-mentioned centrifugal after precipitation add the PBS cushioning liquid of 10mlpH7.0, vibrate desorb 30min in the constant temperature oscillation water-bath of 37 DEG C, collected by centrifugation supernatant, measures the content of each mycotoxin in supernatant, and calculate desorption efficiency R (%)=C
2/ (C
0-C
1) × 100%, C
2for the concentration of mycotoxin each after desorb.
Testing result is as shown in table 1, antifungi toxin feed addictive of the present invention to zearalenone (2 μ g/mL), vomitoxin (5 μ g/mL), AFB1 (AFB1) (0.1 μ g/mL), FT be (0.05 μ g/mL), fumonisin B1 adsorption rate is respectively 93.25%, 95.84%, 97.60%, 97.29% and 96.75%, illustrate that antifungi toxin feed addictive of the present invention can effectively adsorb above-mentioned mycotoxin and be combined with toxin closely, detoxification is more thorough, and it is modified montmorillonite used that suction-operated is obviously better than commonly using.
Table 1 embodiment of the present invention 2 antifungi toxin feed addictive is to the detoxification result of the test of each mycotoxin
two, antifungi toxin feed addictive is on the impact of beef cattle production
Consumption and usage: the feed addictive embodiment of the present invention 2 prepared evenly is mixed in fine fodder according to percentage by weight 5 ‰ and carries out free choice feeding.
Animal used as test and experimental design: choose the hybridization finished cattle 60 introduced from Jiangxi from Xinfeng, Shaoguan De Feng Farming Ltd., be divided into 4 groups at random, often organize 15.
Experimental period: 10 ~ July 10 May
Feeding time and mode: twice daily, morning 8:00 and afternoon 5:00, blue or green herbage material of first being fed by above-mentioned 4 groups of fertile oxen, then feeds the compound of additive prepared by embodiment 1,2 and concentrated feed respectively by embodiment 1 and 2 groups; Blank group is only fed concentrated feed; Comparative example group is fed the compound of the modified montmorillonite used and concentrated feed of the interpolation of equivalent weight.Fine fodder is mainly fed corn flour, wheat bran, dregs of beans etc., is aided with a small amount of sodium acid carbonate, salt.
Measurement parameter: feed intake, weightening finish;
Measuring method: feed intake, then record the concentrated feed mean value that in 1-20,21-40,41-60 day, every consumes.Weighing measurement, the 1st, 21,41,61 day morning 7 empty stomaches weigh, calculate daily gain (see table 2).
Other: morbidity and health condition.
Table 2 antifungi toxin feed addictive is on the impact of beef cattle production
Upper as can be seen from table, embodiment 1 and embodiment group are all better than control group and comparative example group in feed intake and daily gain, wherein embodiment 2 best results in all prescriptions, and daily gain improves about 55%, in pole significant difference (P ﹤ 0.01) than control group.
In addition, control group has 3 ox performances symptom of diarrhea in various degree; Have a head of cattle to show more serious symptom of diarrhea in comparative example, embodiment 1 group has 1 ox mild diarrhea, and embodiment 2 groups all shows in good health.
Above-mentioned experimental result illustrates feeds that feed addictive of the present invention can effectively the present invention can effectively absorbing mycotoxin, the harm that prevents ox feed mold, absorption and releasing mould and mycotoxin from bringing to animal, improve beef cattle feed intake, promote growth of animal, improve animal immune ability, reduce disease to occur, especially to animal alimentary canal diseases, there is obvious prevention and corntrol effect.
Claims (3)
1. an ox antifungi toxin feed addictive, is characterized in that: it is made up of the raw material of following parts by weight: the root of large-flowered skullcap 10 ~ 14 parts, cloves 8 ~ 12 parts, peppermint 8 ~ 12 parts, 12 ~ 18 parts, the leaf of bamboo, Tea Polyphenols 1 ~ 5 part, sodium Diacetate 3 ~ 8 parts, nano montmorillonite 30 ~ 50 parts, active saccharomyces cerevisiae 8 ~ 25 parts.
2. a kind of ox according to claim 1 antifungi toxin feed addictive, is characterized in that: it is made up of the raw material of following parts by weight: the root of large-flowered skullcap 12 parts, cloves 10 parts, peppermint 10 parts, 15 parts, the leaf of bamboo, Tea Polyphenols 1.5 parts, sodium Diacetate 5.5 parts, nano montmorillonite 45 parts, active saccharomyces cerevisiae 20 parts.
3. a kind of ox according to claim 1 and 2 antifungi toxin feed addictive, is characterized in that: the viable bacteria concentration of described active saccharomyces cerevisiae is no less than 2 × 10
10individual/gram.
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| CN111298776B (en) * | 2020-03-03 | 2022-04-26 | 武汉轻工大学 | Magnetic chitin carbonized microsphere adsorbent and preparation method and application thereof |
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| CN112841433A (en) * | 2020-12-31 | 2021-05-28 | 江西正邦动物保健品有限公司 | Feeding composition for improving immunity of swinery and purifying vivotoxin and preparation method thereof |
| CN115428885A (en) * | 2021-06-02 | 2022-12-06 | 菲吉乐科(南京)生物科技有限公司 | Broad-spectrum mildew removing agent capable of simultaneously adsorbing mycotoxin, endotoxin and enterotoxin |
| CN114052130A (en) * | 2021-12-02 | 2022-02-18 | 吉林大学 | Composition for promoting growth of yaks and preparation method thereof |
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