CN103461677A - Mold removal agent for animal feed as well as preparation method and application of mold removal agent - Google Patents
Mold removal agent for animal feed as well as preparation method and application of mold removal agent Download PDFInfo
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Abstract
The invention discloses a mold removal agent for animal feed as well as a preparation method and an application of a mold removal agent. The mold removal agent comprises the following components in parts by mass: 50-150 parts of bacillus subtilis, 5-15 parts of lactobacillus acidophilus freeze-dried powder, 0.1-1 part of sodium selenite, 25-55 parts of vitamin E, 100-300 parts of feed active dry yeast, 5-15 parts of glucomannan, 50-150 parts of montmorillonite and 350-700 parts of modified hydration aluminosilicate. The mold removal agent prepared by the method is environment-friendly and pollution-free, has extremely strong adsorption capacity for various common mycotoxin, forms a stable compound, is not absorbed by intestinal tracts and is excreted from a body along with faeces. The mold removal agent disclosed by the invention can be used for detoxicating, inhibiting the growth of mildews, improving the immunity of animals and promoting the growth of the animals.
Description
Technical field:
The present invention relates to the development of a kind of green, the de-removing mildew of safe microorganisms, be specifically related to de-mould dose of a kind of animal feed and its preparation method and application, mould dose be should take off and improvement hydrated aluminosilicate, vitamin E, bacillus subtilis, lactobacillus acidophilus, glucomannans and feeding active dry yeasr etc. comprised, spice is used, be applicable to various poultry and livestocks, and called after " Mei Tuokang "
Technical background:
Mycotoxin is a series of toxic metabolite products that mould produces in growth course, mainly contain AFB1 (AFB1), zearalenone (ZON), scirpentriol (DON), ochratoxin A (OTA) and fumonisins B1 (FMB1) etc., extensively pollute all feeds and raw material, endanger the development of cultivation, bringing huge economic loss.According to FAO (Food and Agriculture Organization of the United Nation), estimate, the annual whole world has 25% cereal crops to be subject to the pollution of toxin, and the economic loss caused thus reaches hundreds billion of dollars every year.According to incomplete statistics in 2010, only in China, mycotoxin just caused the economic loss up to 5,000,000,000 yuan to pig industry.Jiangsu Province is located in the middle and lower reach of Yangtze River, and the annual plum rain season time is long, temperature is high, and mould losing all easily occurs for the feed of storage and feedstuff.
Animal eat after the feed that contains mycotoxin or food can cause poisoning, can cause lassitude, grow slowly, become thin, appetite subtracts greatly and like drink, discharge feces frequent, rare soft, even suffer from diarrhoea, dewater, incoordination, muscular tremor and even shock and dead, and the death rate is higher.Wherein harm is maximum is the immunosupress of mycotoxin and the immuning failure that causes, causes the popular and great economic loss of disease.Two kinds of toxin of following article are for the impact of animal.
Aflatoxin is a class carcinogenic, the mutagenic matter by force of aspergillus fungi secretion, by aspergillus flavus and aspergillus parasiticus, A.nomius and tetra-kinds of fungies of A.pseudotamarii, is produced.Aflatoxin has separated at present and identifies more than 20 and plant isomers, wherein separated identify the kinds more than 10 such as B1, B2, G1, G2, M1, M2, P1, Q, H1, aflatoxicol are arranged.Common be mainly first 5 kinds, the putting in order as B1 of its toxicity size > M1 > G1 > B2 > G2.Produce malicious aspergillus flavus and only produce B1, two kinds of toxin of B2, but most of aspergillus parasiticus can produce above 6 kinds of toxin.Aflatoxin has induced mutation, Immunosuppression and carcinogenesis, what wherein harm was maximum is AFB1, its toxicity is 10 times of potassium cyanide, 68 times of arsenic, and aflatoxin very easily exists at animal fodders such as corn, peanut, cotton seed and grouts thereof.Aflatoxin can suppress the synthetic of cell DNA, mRNA and protein, can also cause chromosome abnormality, unordered synthetic, the chromosome chain break of the generation of micronucleus, the exchange of sister chromatid, DNA, and form adduct in the mankind and rodent cell.
Because the main target organ of aflatoxin is liver, animal take in after in liver concentration the highest, can cause the acute and chronic hepatotoxicity of livestock and poultry or bring out primary tumor, liver cancer especially, and cause other organ and produce canceration as kidney, lung, stomach, intestines etc.The impact that aflatoxin causes domestic animal is growth retardation, the decline of feed rate, jaundice,, Hypoproteinemia coarse by hair, depression, apocleisis, acute liver disease, liver cancer, immunosupress etc.The impact that poultry is caused is the bursa of farbricius and atrophy of thymus gland, subcutaneous hemorrhage, immune response is poor, resistance descends, the vaccine failure, to the disease competence improve, egg diminishes, egg yolk weight reduction, rate of fertilization, incubation rate reduce, embryonic death increases.Because of aflatoxin toxicity extremely strong, survivable, so the aflatoxin of removing in feed more and more causes people's concern.
Ochratoxin is attack kidney, immune system and hemopoietic system on the impact of domestic animal, the fragility that liver becomes, slight renal lesions, weightening finish decline, anadiosia, growth retardation, azotemia, diuresis, diarrhea, glycosuria disease.The impact that poultry is caused is the inhibition kidney, immunity and hemopoietic system, and Calcium and phosphorous absorption is incomplete, the bone fragility, the eggshell hypocalcification, rate of broken eggs is high, and subcutaneous hemorrhage is easily dampened.
Yet the pollution problem that solves mycotoxin will be set about from many aspects: be one, the hay varieties of cultivating antimycotic toxin; Two, carry out results and storage work, in the General Requirements feed, moisture is lower than 10%; Three, be to adopt physics, chemical or biological method is carried out detoxification to the feed polluted, and improves the utilization rate of feed.No matter whether feed goes mouldy, all to add certain detoxifying agent in actual production.
The domestic research of the poison-removing method to mycotoxin is more late, and systematic understanding is not yet universal.Obtained certain achievement through development for many years, method comprises physical method, chemical method, biological method etc.The method of physics: manually pick musty grain, ultraviolet inactivation mycotoxin, absorption detoxicity method and hot deactivation etc.; The method of chemistry: ammonification deactivation etc.; Biological method: reduce toxicity, microorganism deactivated mycotoxin and enzyme preparation detoxification etc.Although method has a lot, consider large production and the simplicity problem of practical operation, available have physical absorption detoxification and a biological detoxication.
The absorption detoxicity method is both economical, effective and has the method for realistic meaning.The absorption detoxicity method is to attract the polar functional group of mycotoxin by adsorbent, by their absorption, catch and fix, the absorption of prevention body contratoxin, thus the reduction toxin to the harm of body and toxin residual in agricultural product.The absorption detoxicity method is promoted in a big way in production practices, various natural or artificial synthetic mycotoxin adsorbents occurred in the market.In prior art, adsorbent commonly used has hydration ca aluminosilicate sodium salt (HSCAS), montmorillonite, bentonite, clay, zeolite and active carbon etc.But adopt existing de-mould dose to have following technical problem: conventional de-mould dose just is conceived to deviating from of mycotoxin, and has ignored the inhibition to fungus growth, de-mould dose of absorption has saturated and halfway the time, so cures the symptoms, not the disease.
Summary of the invention:
The object of the present invention is to provide de-mould dose of a kind of animal feed.
Another object of the present invention is to provide the preparation method of de-mould dose of a kind of animal feed.
Another purpose of the present invention is to provide the application of de-mould dose of a kind of animal feed.
Purpose of the present invention is achieved through the following technical solutions:
De-mould dose of a kind of animal feed, should take off mould dose and comprise according to the mass fraction following component: 50～150 parts of bacillus subtilises (CICC20872), 5～15 parts, lactobacillus acidophilus freeze-drying powder (CICC6074), 0.1～1 part of sodium selenite, 5～55 parts of vitamin E2s, 100～300 parts of feeding active dry yeasts, 5～15 parts of glucomannans, 350～700 parts of 50～150 parts of montmorillonites and improvement hydrated aluminosilicates.
Preferably: in this de-mould dose, the content of each component is: 70～130 parts of bacillus subtilises (CICC20872), 7～13 parts, lactobacillus acidophilus freeze-drying powder (CICC6074), 0.2～0.8 part of sodium selenite, 30～50 parts of vitamin Es, 140～260 parts of feeding active dry yeasts, 7～13 parts of glucomannans, 400～650 parts of 70～130 parts of montmorillonites and improvement hydrated aluminosilicates.
Further preferred, in should taking off mould dose, the content of each component was: 100 parts of bacillus subtilises (CICC20872), 10 parts, lactobacillus acidophilus freeze-drying powder (CICC6074), 0.55 part of sodium selenite, 40 parts of vitamin Es, 200 parts of feeding active dry yeasts, 10 parts of glucomannans, 540 parts of 100 parts of montmorillonites and improvement hydrated aluminosilicates.
Described improvement hydrated aluminosilicate adopts the following methods preparation: by the hydrated aluminosilicate mineral, the process fine crusher is broken and sieve to such an extent that particle diameter is 3～10mm mineral, then on agate jar, ground, load volume is 1:1.5～3 with the tank volume ratio, adopt the mode of dry-wet ball mill, front 25～35h adopts the dry type ball-milling method to grind, and rear 25～35h adds absolute ethyl alcohol to adopt the wet ball-milling method to grind, and after grinding, sample is dried and obtained the improvement hydrated aluminosilicate.
The addition of above-mentioned absolute ethyl alcohol is: every 50g mineral add the absolute ethyl alcohol of 10～20ml.
Described improvement hydrated aluminosilicate is preferably improvement hydration ca aluminosilicate sodium salt.
The preparation method that above-mentioned animal feed is de-mould dose comprises the following steps:
(1) preparation improvement hydrated aluminosilicate: by the hydrated aluminosilicate mineral, the process fine crusher is broken and sieve to such an extent that particle diameter is 3～10mm mineral, then on agate jar, ground, load volume is 1:1.5～3 with the tank volume ratio, adopt the mode of dry-wet ball mill, front 25～35h adopts the dry type ball-milling method to grind, and rear 25～35h adds absolute ethyl alcohol to adopt the wet ball-milling method to grind, and after grinding, sample is dried and obtained the improvement hydrated aluminosilicate;
(2) by bacillus subtilis (CICC20872), lactobacillus acidophilus freeze-drying powder (CICC6074), sodium selenite, vitamin E, feeding active dry yeast, glucomannans, improvement hydrated aluminosilicate prepared by montmorillonite and step (1) mixes in proportion.
Above-mentioned preparation method, its addition that is described absolute ethyl alcohol is: every 50g mineral add the absolute ethyl alcohol of 10～20ml.
Above-mentioned animal feed takes off the mould dose of application in animal feed.
The content of bacillus subtilis (CICC20872) in above-mentioned de-mould dose is 1 * 10
10cfu/g, the content of lactobacillus acidophilus freeze-drying powder (CICC6074) is 2 * 10
10cfu/g, vitamin E is 500IU/g.
What the present invention developed is that a kind of green, safe microorganism take off mould dose.Should the de-mould dose of addition in feed be 0.05%～0.2%, in the feed of 1 ton, add de-mould dose of 0.5～2kg.
The formula that the present invention is de-mould dose mainly contains two large class components and forms, and the one, the composition that can directly absorb mycotoxin comprises glucomannans, montmorillonite, improvement hydrated aluminosilicate; Equations of The Second Kind be can mould fungus inhibition growth and flora and the trace element of regulating animal intestinal comprise bacillus subtilis, lactobacillus acidophilus, sodium selenite, vitamin E.Wherein the feeding active dry yeasr in the formula has absorption concurrently and regulates animal intestinal and promotes the growth double action.
Each material in glucomannans, montmorillonite and improvement hydrated aluminosilicate all has the effect of absorbing mycotoxin, but the kind of its adsorption effect and absorbing mycotoxin is limited, but the inventor combines the above four kinds compositions with de-mould function, can produce synergistic function, the adsorbable overwhelming majority's mycotoxin and its adsorption effect significantly increase.
For the better effect of performance hydrated aluminosilicate, the In Natural Silicate salt material is carried out to improved treatment, not only make hole increase, increased the area of absorption, also increased the speed of its absorption.
Adsorbent can take off mould, but growth that can not mould fungus inhibition can not suppress the generation of toxin, and in order to control from source, we have added the adaptable microorganism of feed.These two kinds of bacteriums enter ramp afterwards in body, change intestinal environment, suppress the growth of various pathogens, produce various enzymes, acid and vitamin simultaneously and help animal digestion and absorption.Add feeding active dry yeasr, sodium selenite and vitamin E in the prescription simultaneously, for alleviating the bad reaction of animal, improved its immunity.
This prescription is an organic whole, can not see the function of certain single component, removes any component in prescription, all can destroy integral body.This prescription not only can remove various mycotoxins, can also promote the growth of animal.Each component in this prescription has the effect of Synergistic.The growth of its detoxification, mould fungus inhibition and raising animal immunizing power promote the resultant effect of growth of animal all to be significantly higher than the effect sum that each component is used separately.
The various common mycotoxins of glucomannans, montmorillonite and improvement aluminium hydrosilicate salt pair that add in the present invention's prescription all have extremely strong absorption affinity as aflatoxin, brown aspertoxin, FT, excellent aspertoxin, zearalenone, vomiting mycin, T-2 toxin and rotten Ma Su etc., and form stable compound, not by intestinal absorption, with ight soil, excrete.
The aluminium silicate minerals such as montmorillonite, bentonite, clay are as non-nutritive additive, by a large amount of absorption for the feed mycotoxin.Mechanism about aluminosilicate absorption mycotoxin toxicity, be commonly considered as because aluminosilicate has adsorbed mycotoxin and formed stable compound, stoped the absorption of mycotoxin in animal alimentary canal, thereby reduced the toxin amount entered in animal body, made its toxicity alleviate or eliminate.These aluminium silicate mineral materials have that hole is many, surface area is large and the characteristic such as ion-exchange capacity is strong, and mycotoxin is had to certain suction-operated.Yet these common aluminosilicate material adsorption capacities are limited, and selectively poor.As the hydration ca aluminosilicate sodium salt (HSCAS) extracted from zeolite has selective adsorption capacity preferably to AFB1, but not very good to other several main mycotoxin adsorption capacities, as not good as the adsorption effect to ZON, OTA, T-2 and DONN.Another shortcoming of HSCAS adsorbent is that it has certain adsorption capacity to vitamin and mineral salt.
In order to overcome above shortcoming, we find after the experimental study, by the applying nano technology, HSCAS are carried out can improving suction-operated and the selective adsorption capacity of HSCAS to nonpolar toxin after modification.HSCAS nano-complex aperture after modification increases and distribution rule, and specific area increases, and has greatly improved its adsorptive selectivity to adsorbed material, selectivity and high efficiency.Mycotoxin in this novel nano compound energy efficient adsorption feed, stop it to enter in body by the absorption approach, and excrete from ight soil, thereby reduce residual in the animal body tissue of toxin, alleviates the harmful effect of toxin to growth of animal.
Iturin is the large class lipopeptide compound produced by hay bacillus, and the family member comprises Iturin A, B, C, D, E, gemma rhzomorph (Bacillomycin) D, F, L and mycosubtilin (Mycosubtilin) etc.The peptide ring that they all are comprised of 7 amino acid residues, and the amphoteric compound of beta-amino aliphatic acid side chain composition, they have strong antibacterial ability to most pathogenic yeast and mould.Bacillus subtilis can produce gemma mycin D, suppresses Aspergillus flavus and produces aflatoxin.The antibacterial substance that producing bacillus subtilis is given birth to has heat resistance preferably, if the active material of its secretion can be carried out to Purification and Characterization, and the control for aflatoxin contamination by its active material, will bring huge benefit to the production of crops.
Exist with spore form with bacillus subtilis at storage, in the period of dormancy, do not consume the nutritional labeling of feed, do not affect feed quality, quality of the fodder is guaranteed.Bacillus subtilis not only can the mould fungus inhibition toxin generation, also growth of animal is had to a lot of facilitations.
Bacillus sprouts and changes the auxotype thalline with metabolism into rapidly in enteron aisle, consumes a large amount of oxygen, maintains the tight oxygen environment of enteron aisle.Bacillus can produce pepsin, lipase, amylase activity, energy degradation of xylan, CMC, polygalacturonase and some other complicated vegetality carbohydrates, the secretion organic acid promotes the breeding of beneficial bacterium etc., so bacillus is a kind of feed addictive of pre-anti-diarrhea.Can also reduce the discharge of foul smell in addition.
Yeast also can adsorb mycotoxin, and under acid condition, yeast is better to the assimilation effect of ochracin.The absorption affinity of yeast cell wall is especially obvious, because abundant peptide glycan, protein and lipid in cell membrane provides for absorbing mycotoxin the adsorption site that kind is various, mechanism is different.P450 in saccharomyces cerevisiae can, in conjunction with aflatoxin, can reduce the content of B1, B2, G1, G2 effectively.
Lactobacillus acidophilus produces lactic acid and acetic acid, and the energy extracellular proteinase, promote digesting and assimilating of protein.The predigestion of food in stomach be can improve, calcium, phosphorus, the utilization rate of iron and the absorption of vitamin D improved; Also can produce antibiotic, as acidophilin, acidolin and lactocidin.The a large amount of Cobastab group energys that produce promote the growth of nerve cell.
Therefore the present invention utilizes above several composition to make de-mould dose of a kind of green non-pollution.
The characteristics that the present invention is de-mould dose are exactly that the multiple component with the various toxin functions of absorption forms, and can adsorb common toxin.Another characteristics that the present invention is de-mould dose have added the various active bacterium exactly, and the growth that these bacteriums not only can mould fungus inhibition, the more important thing is that its secretion can promote the growth of animal, regulates functions of intestines and stomach, strengthens the immunity of animal.These bacteriums are all to buy from industrial microorganism preservation center, can be applied directly in feed.
Beneficial effect of the present invention:
The de-mould dose of green non-pollution that the present invention is prepared, all have extremely strong absorption affinity to various common mycotoxins, and form stable compound, not by intestinal absorption, with ight soil, excretes.Of the present invention de-mould dose not only can detoxification, growth that can also mould fungus inhibition, and improve animal immunizing power and promote growth of animal.
The specific embodiment:
Embodiment 1 prepares de-mould dose of animal feed
Preparation improvement hydration ca aluminosilicate sodium salt: at first by hydration ca aluminosilicate sodium salt mineral (purchased from the white Co., Ltd in U.S. Mill, effective content is more than 95%) then sieving obtains the mineral of 3～10mm to carry out fragmentation (the fertile powerful jaw crusher PEW180 of Shanghai dimension * 1300) through fine crusher, then on agate jar (Nanjing Sang Li Electronic Equipment Factory produces the QM-4H ball mill), ground, loading is 1:2 with the tank volume ratio, adopt the mode of dry-wet ball mill, front 30h adopts dry type ball-milling method (rotating speed is 200r/min) to grind, rear 30h adopts the wet ball-milling method to grind, method is with the dry type ball-milling method, the absolute ethyl alcohol (amount that every 50g mineral add) that adds 12～15ml while just grinding, after grinding, sample is dried.Adopt the 2000F of NEC type transmission microscopy to observe the particle ground, what the particle of discovery≤100nm accounted for 78%, 100～150nm accounts for 7%, and>150nm accounts for 15%.This method can make the particle that reaches Nano grade account for more than 85%, so the method can prepare nano level particle, improves hydration ca aluminosilicate sodium salt.
Take each material and mixed by following consumption: bacillus subtilis (CICC20872) 100g(content is 1 * 10
10cfu/g), lactobacillus acidophilus freeze-drying powder (CICC6074) 10g(content is 2 * 10
10cfu/g), sodium selenite 0.55g, vitamin E 40g(500IU/g), feeding active dry yeast 200g(Angel Yeast Co.,Ltd), glucomannans 10g, montmorillonite 100g, improvement hydration ca aluminosilicate sodium salt 540g.
The main component of table 1 0.1% feed addictive forms
0.1% feed addictive, 1kg adds in the feed of 1 ton.
Comparative example 1
Take each material and mixed by following consumption: bacillus subtilis (CICC20872) 100g(content is 1 * 10
10cfu/g), lactobacillus acidophilus freeze-drying powder (CICC6074) 10g(content is 2 * 10
10cfu/g), sodium selenite 0.55g, vitamin E 40g(500IU/g), feeding active dry yeast 200g(Angel Yeast Co.,Ltd), glucomannans 10g, montmorillonite 100g, natural hydration ca aluminosilicate sodium salt 540g.
Comparative example 2
Take each material and mixed by following consumption: bacillus subtilis (CICC20872) 100g(content is 1 * 10
10cfu/g), lactobacillus acidophilus freeze-drying powder (CICC6074) 10g(content is 2 * 10
10cfu/g), sodium selenite 0.55g, vitamin E 40g(500IU/g), feeding active dry yeast 200g(Angel Yeast Co.,Ltd), montmorillonite 100g.
Test one
1 materials and methods
1.1 test period and place
This test on June 7th, 1 2011 on April 20th, 2011 in Shandong Province certain chicken house carry out.
1.2 experimental design
Select the AA+ meat kind chicken in 12300 70 week ages, be equally divided at random test group, control group one, control group two, control group three and blank group, every group of 3 repetitions, free choice feeding, drinking-water are raised in identical environment.
Test group: basal diet (in Table 2), add mould dose of 0.1% Mei Tuokangtuo (start to add on May 8th, 2011, add 4 weeks) prepared by embodiment 1;
Control group one: basal diet, add mould complete mould de-mould dose (Beijing Nong Bolier science and technology limited Company lot number 20110304) by 0.1% amount, on May 8th, 2011, start to add, add 4 weeks;
Control group two: basal diet, 0.1% de-mould dose (start to add on May 8th, 2011, add 4 weeks) of adding comparative example 1 preparation;
Control group three: basal diet, 0.1% de-mould dose (start to add on May 8th, 2011, add 4 weeks) of adding comparative example 2 preparations;
Blank group: basal diet (not adding de-mould dose).
Mould complete mould de-mould dose be Beijing Nong Bolier science and technology limited Company production, and the product main component is Meng Tuo city, imported France original producton location, nanometer montomorillonite, lactic acid second fat, sweet poly oligosaccharide, potassium sorbate, ablastins, flavor enhancement etc.
1.3 test index is measured
Duration of test records and measures the dead number of washing in a pan of each group kind of chicken every day, the number of laying eggs, defective egg number (broken, abnormal, soft without shell), select egg by kind of an egg standard, qualified hatching of breeding eggs, mensuration with and every production performance index and reproductive performance, comprise egg number, qualified kind of egg number, rate of fertilization, incubation rate, strong young rate, still birth rate etc., and analyze its variation tendency.
Fertility rate of hatching egg=fertile egg number/enter incubation number * 100% hatchability of fertile eggs=chickling-surviving number/fertile egg number * 100%
Strong young number/chickling-surviving number * 100% still birth rate=stillborn foetus egg number/fertile egg number * 100% of strong young rate=always
Blood sampling before adding de-mould dose is simultaneously taken a blood sample weekly 1 time later, totally 4 times, by the HI method, censorship serum is carried out the detection of newcastle epidemic disease antibody level, and then analyzes and infer improving the impact of immunity of organisms aspect.
2 result of the tests
2.1. add the de-mould dose of different impacts on meat kind chicken production performance
As shown in Table 3, the average egg number of test group is 1632.60 pieces, and the average egg number of control group one, control group two and control group three is respectively 1612.74,1607.2,1600.3 pieces, and it is 1592.50 that the blank group is on average laid eggs.With respect to the blank group, test group and control group one, control group two, average increase every day kind of the egg number of control group three are 40.1 and 20.24,14.7,7.8 pieces, have improved respectively 2.52% and 1.27%, 0.92%, 0.49%.Plant the disqualification rate of egg with respect to the blank group, test group and control group one, control group two and control group three have descended respectively 21.4% and 12.76%, 8.65%, 6.12%.
From strong young data, with respect to the blank group, test group and control group one, control group two and control group three have risen respectively 13.37% and 8.9%, 7.5%, 3.1%.The stillborn foetus number is with respect to the blank group, and test group and control group one, control group two and control group three have descended respectively 21.6% and 15.1%, 13.4%, 12.3%.Therefore from above data, use de-mould dose of ratio do not use de-mould dose egg number, qualified egg number, healthy chicken or aspect survival rate all tool improve a lot.From data, use the effect of mould dose of the Mei Tuokangtuo of embodiment 1 preparation to significantly improve than the effect of using mould complete mould de-mould dose and comparative example 1, comparative example 2.
Table 2 basal diet component list
Table 3 takes off the mould dose of impact on meat chicken production performance
2.2 add the de-mould dose of impact on meat kind chicken immune performance.
By table 4, can find out, add de-mould dose after the 3rd week, compare the blank group, the antibody titer of ND of test group and control group one, control group two and control group three meat kind chickens has improved respectively 1.4 and 1.1,0.7,0.3.
Table 4 is used difference to take off mould dose for kind of the impact of antibody IgY against chicken Newcastle Disease level
3 conclusions
Can find out that from this experiment de-mould dose is laid eggs for kind of a chicken, the health of chick has important impact, therefore no matter be whether mycotoxin is arranged in feed, add the de-mould dose of growth for kind of chicken, lay eggs and all there is positive effect, from above experimental result, also can find out, the effect of de-mould dose that the effect that the Mei Tuokangtuo that we research and develop is mould dose will be compared to contrast is significantly increased.The effect of de-mould dose that adopts improvement hydration ca aluminosilicate sodium salt to make is significantly increased than the effect of de-mould dose that adopts natural hydration ca aluminosilicate sodium salt to make.The effect of glucomannans, montmorillonite and three kinds of adsorbent Integrated usings of improvement hydrated aluminosilicate is significantly higher than any two kinds of a kind of adsorbent wherein and is used in combination or the effect of single use.
Test two
1. test material and method
1.1 test period and place
Test is carried out on one pig farm in Shandong, and the time is in May, 2012
1.2 experimental design
The random selection is in a good state of health, and body condition is standard type, 120 of the assorted sows of the long white Yorkshire binary of the multiparity of parity between 3-5 time.Be divided at random 3 groups, be respectively test group, control group and blank group, each group is respectively 40 repetitions, and each repeats 1 sow.
The sow of test group is pressed mould dose of the Mei Tuokangtuo that 1kg/ ton addition adds embodiment 1 preparation;
Control group adds except de-mould dose of mould growth encourage expert (the excellent profit in Beijing is protected biotechnology Co., Ltd lot number 20120304);
The blank group is not added de-mould dose.
The sow of test group, control group and blank group is all processed since the gestational period, to next tire weaning period end.Sow all adopts position limiting fence to raise, and breeding wony adopts test-tube mode to carry out.Feed sow nonpregnant every day in stage the 2.25-2.5kg feed, feed the 2-2.25kg feed after breeding in 20d, the scale of feeding that after breeding, 2ld returns to 2.25-2.5kg is until conceived 80d, and conceived 8ld starts scale of feeding and brings up to 3-3.5kg.
1.3 comprise for the sow reproductive performance index observing in test:
The endometritis incidence of disease: the sow that still has lochia discharge and secretion to be muddy shape over 5d is recorded as endometritis, and carries out administration in uterine irrigation and uterus and treated.Aborning, there is the part sow that obvious endometritis symptom is arranged, but Repeat breeding, and usually in oestrus, in vagina, flow out the muddy liquid frowzy of being with, also be defined as endometritis for these sows; Oestrus of sow rate after wean: occur in 15d that from weaning the sow that oestruses and breed accounts for the number of total sow; The postweaning estrus time interval: refer to sow from the wean to the time interval of oestrusing; The Farrowing rate: the parturient sow number accounts for the percentage of total breeding sow number.Litter size: whole piglet numbers that the sow brood is given birth to comprise stillborn foetus and mummy tire.The number of live birth: sow gives birth to a brood of piglet number whole alive.Survival rate: the number of live birth accounts for the percentage of nest litter size.The average weaning weight of nest piglet: piglet is weaned during 28 age in days after birth, whole nest piglet body weight/piglet number.
2 experimental results
2.1 the de-mould dose of impact on the Gilt Uterus intimitis incidence of disease
Add in 40 sows of test group of mould dose of Mei Tuokangtuo, the sow number that endometritis occurs is 2, and the incidence of disease is 5%.A sow number of control group generation endometritis is 5, and the incidence of disease is 12.5%.And the pig's head number of blank group generation endometritis is 6, the incidence of disease is 15%, can find out and add the de-mould dose of incidence of disease that can significantly reduce endometritis from these group data, and U.S. holder Kombi will be got well except de-mould dose of mould growth encourage expert's effect.
Table 5 sow basal diet proportioning
Compound additive provides for every Kg daily ration: vitamin A, 5512IU; Cholecalciferol, 1100IU; Vitamin E, 48IU; Prokeyvit, 2.2mg; Cobalamin, 27.6ug; Riboflavin, 5.5mg; D-VB5,13.8mg; Nicotinic acid, 30.3mg
Table 6 takes off the mould dose of impact on the Gilt Uterus intimitis incidence of disease
2.2 de-mould dose on sow postweaning estrus rate and estrus time interval impact
40 sows of test group of adding mould dose of Mei Tuokangtuo have 35 and oestrus in 15d, and the rate of oestrusing is 87.5%; And 40 sows of control group of adding except de-mould dose of mould growth encourage expert have 32 oestrus of sow, the rate of oestrusing is 80%.40 sows of blank group only have 31 hair feelings, and the rate of oestrusing is only 77.5%.
Table 7 takes off mould dose to oestrus of sow rate and the impact in the postweaning estrus time interval
2.3 the de-mould dose of impact on Farrowing rate and nest litter size
In process of the test, to oestrus undesired and breed unsuccessfully because selected sow exists, each sow number of organizing the actual participation statistics is respectively that test group is 35,32 of control groups, the blank group is 31.Wherein test group sow totally 32 childbirths, parturition rate is 91.4%, control group totally 27 smoothly deliver, parturition rate is 84.3%, blank group parturition rate is 81.2%.Therefore from following table, can find out, no matter be from the nest litter size or from the survival rate aspect, a group of adding de-mould dose is all effective than one group that does not add de-mould dose, and the ratio that adds Mei Tuokang adds effective except de-mould dose of mould growth encourage expert
Table 8 takes off the mould dose of impact on Farrowing rate and nest litter size
3 conclusions
Take off as can be seen from the above data mould dose for sow, the piglet tool has a great impact, and therefore, in actual production, no matter whether there is mycotoxin in feed, all will add the de-mould dose of generation that not only can prevent above pathology, can also increase economic efficiency.
Embodiment 2
Take each material and mixed by following consumption: bacillus subtilis (CICC20872) 70g(content is 1 * 10
10cfu/g), lactobacillus acidophilus freeze-drying powder (CICC6074) 7g(content is 2 * 10
10cfu/g), sodium selenite 0.2g, vitamin E 30g(500IU/g), feeding active dry yeast 140g(Angel Yeast Co.,Ltd), glucomannans 7g, montmorillonite 70g, the improvement hydration ca aluminosilicate sodium salt 400g prepared according to the described step of embodiment 1.
Embodiment 3
Take each material and mixed by following consumption: bacillus subtilis (CICC20872) 130g(content is 1 * 10
10cfu/g), lactobacillus acidophilus freeze-drying powder (CICC6074) 13g(content is 2 * 10
10cfu/g), sodium selenite 0.8g, vitamin E 50g(500IU/g), feeding active dry yeast 260g(Angel Yeast Co.,Ltd), glucomannans 13g, montmorillonite 130g, the improvement hydration ca aluminosilicate sodium salt 650g prepared according to the described step of embodiment 1.
Embodiment 4
Take each material and mixed by following consumption: bacillus subtilis (CICC20872) 50g(content is 1 * 10
10cfu/g), lactobacillus acidophilus freeze-drying powder (CICC6074) 5g(content is 2 * 10
10cfu/g), sodium selenite 0.1g, vitamin E2 5g(500IU/g), feeding active dry yeast 100g(Angel Yeast Co.,Ltd), glucomannans 5g, montmorillonite 50g, the improvement hydration ca aluminosilicate sodium salt 350g prepared according to the described step of embodiment 1.
Embodiment 5
Take each material and mixed by following consumption: bacillus subtilis (CICC20872) 150g(content is 1 * 10
10cfu/g), lactobacillus acidophilus freeze-drying powder (CICC6074) 15g(content is 2 * 10
10cfu/g), sodium selenite 1g, vitamin E 55g(500IU/g), feeding active dry yeast 300g(Angel Yeast Co.,Ltd), glucomannans 15g, montmorillonite 150g, the improvement hydration ca aluminosilicate sodium salt 700g prepared according to the described step of embodiment 1.
Claims (9)
1. an animal feed takes off mould dose, it is characterized in that: this de-mould dose comprises following component according to the mass fraction: 50～150 parts of bacillus subtilises (CICC20872), 5～15 parts, lactobacillus acidophilus freeze-drying powder (CICC6074), 0.1～1 part of sodium selenite, 5～55 parts of vitamin E2s, 100～300 parts of feeding active dry yeasts, 5～15 parts of glucomannans, 350～700 parts of 50～150 parts of montmorillonites and improvement hydrated aluminosilicates.
2. animal feed according to claim 1 is de-mould dose, it is characterized in that: in this de-mould dose, the content of each component is: 70～130 parts of bacillus subtilises (CICC20872), 7～13 parts, lactobacillus acidophilus freeze-drying powder (CICC6074), 0.2～0.8 part of sodium selenite, 30～50 parts of vitamin Es, 140～260 parts of feeding active dry yeasts, 7～13 parts of glucomannans, 400～650 parts of 70～130 parts of montmorillonites and improvement hydrated aluminosilicates.
3. animal feed according to claim 2 is de-mould dose, it is characterized in that: in this de-mould dose, the content of each component is: 100 parts of bacillus subtilises (CICC20872), 10 parts, lactobacillus acidophilus freeze-drying powder (CICC6074), 0.55 part of sodium selenite, 40 parts of vitamin Es, 200 parts of feeding active dry yeasts, 10 parts of glucomannans, 540 parts of 100 parts of montmorillonites and improvement hydrated aluminosilicates.
4. animal feed according to claim 1 is de-mould dose, it is characterized in that described improvement hydrated aluminosilicate adopts the following methods preparation:
By the hydrated aluminosilicate mineral, the process fine crusher is broken and sieve to such an extent that particle diameter is 3～10mm mineral, then on agate jar, ground, load volume is 1:1.5～3 with the tank volume ratio, adopt the mode of dry-wet ball mill, front 25～35h adopts the dry type ball-milling method to grind, and rear 25～35h adds absolute ethyl alcohol to adopt the wet ball-milling method to grind, and after grinding, sample is dried and obtained the improvement hydrated aluminosilicate.
5. animal feed according to claim 4 is de-mould dose, and it is characterized in that the addition of described absolute ethyl alcohol is: every 50g mineral add the absolute ethyl alcohol of 10～20ml.
6. according to de-mould dose of the described animal feed of any one in claim 1～4, it is characterized in that described improvement hydrated aluminosilicate is for improvement hydration ca aluminosilicate sodium salt.
7. in claim 1～3, the described animal feed of any one takes off the preparation method of mould dose, it is characterized in that comprising the following steps:
(1) preparation improvement hydrated aluminosilicate: by the hydrated aluminosilicate mineral, the process fine crusher is broken and sieve to such an extent that particle diameter is 3～10mm mineral, then on agate jar, ground, load volume is 1:1.5～3 with the tank volume ratio, adopt the mode of dry-wet ball mill, front 25～35h adopts the dry type ball-milling method to grind, and rear 25～35h adds absolute ethyl alcohol to adopt the wet ball-milling method to grind, and after grinding, sample is dried and obtained the improvement hydrated aluminosilicate;
(2) by bacillus subtilis (CICC20872), lactobacillus acidophilus freeze-drying powder (CICC6074), sodium selenite, vitamin E, feeding active dry yeast, glucomannans, improvement hydrated aluminosilicate prepared by montmorillonite and step (1) mixes in proportion.
8. preparation method according to claim 7, it is characterized in that the addition of described absolute ethyl alcohol is: every 50g mineral add the absolute ethyl alcohol of 10～20ml.
9. the de-mould dose of application in animal feed of the described animal feed of any one in claim 1～4.
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