CN103461677B - Mold removal agent for animal feed as well as preparation method and application of mold removal agent - Google Patents
Mold removal agent for animal feed as well as preparation method and application of mold removal agent Download PDFInfo
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Abstract
The invention discloses a mold removal agent for animal feed as well as a preparation method and an application of a mold removal agent. The mold removal agent comprises the following components in parts by mass: 50-150 parts of bacillus subtilis, 5-15 parts of lactobacillus acidophilus freeze-dried powder, 0.1-1 part of sodium selenite, 25-55 parts of vitamin E, 100-300 parts of feed active dry yeast, 5-15 parts of glucomannan, 50-150 parts of montmorillonite and 350-700 parts of modified hydration aluminosilicate. The mold removal agent prepared by the method is environment-friendly and pollution-free, has extremely strong adsorption capacity for various common mycotoxin, forms a stable compound, is not absorbed by intestinal tracts and is excreted from a body along with faeces. The mold removal agent disclosed by the invention can be used for detoxicating, inhibiting the growth of mildews, improving the immunity of animals and promoting the growth of the animals.
Description
Technical field:
The present invention relates to a kind of green, development that safe microorganisms takes off removing mildew, be specifically related to a kind of animal feed and take off mould dose and its preparation method and application, this de-mould dose comprises improvement hydrated aluminosilicate, vitamin E, bacillus subtilis, lactobacillus acidophilus, glucomannans and feeding active dry yeasr etc., spice uses, be applicable to various poultry and livestock, and called after " Mei Tuokang "
Technical background:
Mycotoxin is a series of toxic metabolic products that mould produces in growth course, mainly contain AFB1 (AFB1), zearalenone (ZON), scirpentriol (DON), ochratoxin A (OTA) and fumonisins B1 (FMB1) etc., extensive pollution all feeds and raw material, endanger the development of cultivation, bring huge economic loss.Estimate according to FAO (Food and Agriculture Organization of the United Nation), the annual whole world has the cereal crops of 25% to be subject to the pollution of toxin, and the economic loss caused thus reaches hundreds billion of dollar every year.According to incomplete statistics in 2010, only in China, mycotoxin just causes the economic loss up to 5,000,000,000 yuan to pig industry.Jiangsu Province is located in the middle and lower reach of Yangtze River, and the annual plum rain season time is long, temperature is high, and mould losing all easily occurs for the feed of storage and feedstuff.
Animal can cause poisoning after eating feed containing mycotoxin or food, lassitude can be caused, grow slowly, become thin, appetite subtract greatly and like drink, discharge feces frequent, rare soft, even suffer from diarrhoea, dewater, incoordination, muscular tremor and even shock and dead, and the death rate is higher.Wherein endanger and maximum be the immunosupress of mycotoxin and the immuning failure caused, cause the popular and great economic loss of disease.Mainly introduce the impact of two kinds of toxin for animal below.
Aflatoxin is a class carcinogenic, the mutagenic matter by force of aspergillus fungi secretion, is produced by aspergillus flavus and aspergillus parasiticus, A.nomius and A.pseudotamarii tetra-kinds of fungies.Aflatoxin has been separated at present and has identified more than 20 and plant isomers, be wherein separated identify have the kinds more than 10 such as B1, B2, G1, G2, M1, M2, P1, Q, H1, aflatoxicol.Common be mainly first 5 kinds, putting in order as B1>M1>G1>B2Gre atT.GreaT.GTG2 of its toxicity size.Produce malicious aspergillus flavus and only produce B1, B2 two kinds of toxin, but most of aspergillus parasiticus can produce above 6 kinds of toxin.Aflatoxin has induced mutation, Immunosuppression and carcinogenesis, wherein endangering maximum is AFB1, its toxicity is 10 times of potassium cyanide, 68 times of arsenic, and aflatoxin very easily exists at animal fodders such as corn, peanut, cotton seed and grouts thereof.The synthesis of aflatoxin energy T suppression cell DNA, mRNA and protein, chromosome abnormality can also be caused, the unordered synthesis of the generation of micronucleus, the exchange of sister chromatid, DNA, chromosome chain break, and adduct is formed in the mankind and rodent cell.
First target organs due to aflatoxin is liver, and it is the highest that animal takes in concentration in rear liver, can cause the acute and chronic hepatotoxicity of livestock and poultry or bring out primary tumor, especially liver cancer, and cause other organ as generation cancerations such as kidney, lung, stomach, intestines.Aflatoxin is growth retardation, the decline of feed rate, jaundice, Hypoproteinemia, depression, apocleisis, acute liver disease, liver cancer coarse by hair, immunosupress etc. on the impact that domestic animal is caused.The impact caused poultry is the bursa of farbricius and atrophy of thymus gland, subcutaneous hemorrhage, immune response is poor, resistance declines, vaccine is failed, improve disease competence, egg diminishes, egg yolk weight reduces, rate of fertilization, incubation rate reduce, embryonic death increases.Because aflatoxin toxicity is extremely strong, survivable, so the aflatoxin removed in feed more and more causes the concern of people.
Ochratoxin is attack kidney, immune system and hemopoietic system on the impact of domestic animal, the fragility that liver becomes, slight renal lesions, weightening finish decline, anadiosia, growth retardation, azotemia, diuresis, diarrhea, glycosuria disease.The impact caused poultry is suppression kidney, and immunity and hemopoietic system, Calcium and phosphorous absorption is complete, and bone is fragile, and eggshell hypocalcification, rate of broken eggs is high, subcutaneous hemorrhage, easily dampens.
But the pollution problem that will solve mycotoxin will be set about from many aspects: one, be the hay varieties of cultivating antimycotic toxin; Two, carry out results and storage work, in General Requirements feed, moisture is lower than 10%; Three, be adopt physics, chemical or biological method carries out detoxification to the feed polluted, and improves the utilization rate of feed.In actual production, no matter whether feed goes mouldy, and all will add certain detoxifying agent.
The domestic research of the poison-removing method to mycotoxin is more late, and systematic understanding is not yet universal.Development through for many years achieves certain achievement, and method comprises physical method, chemical method, biological method etc.The method of physics: manually pick musty grain, ultraviolet inactivation mycotoxin, absorption detoxicity method and hot deactivation etc.; The method of chemistry: ammonification deactivation etc.; Biological method: fermentation detoxification, microorganism deactivated mycotoxin and enzyme preparation detoxification etc.Although method has a lot, consider the simplicity problem of large production and practical operation, available has physical absorption detoxification and biological detoxication.
Absorption detoxicity method is both economical, effective and has the method for realistic meaning.Absorption detoxicity method attracts the polar functional group of mycotoxin by adsorbent, their adsorbed, catch and fix, stop the absorption of body contratoxin, thus reduce toxin to the harm of body and toxin remaining in agricultural product.Absorption detoxicity method is promoted in a big way in production practices, to occur the mycotoxin absorbant of various natural or Prof. Du Yucang in the market.Adsorbent conventional in prior art has hydration ca aluminosilicate sodium salt (HSCAS), montmorillonite, bentonite, clay, zeolite and active carbon etc.But adopt existing de-mould dose to there is following technical problem: conventional de-mould dose is just conceived to deviating from of mycotoxin, and have ignored the suppression to fungus growth, and de-mould dose is adsorbed with saturated with time halfway, therefore cures the symptoms, not the disease.
Summary of the invention:
A kind of animal feed is the object of the present invention is to provide to take off mould dose.
Another object of the present invention is to the preparation method providing a kind of animal feed to take off mould dose.
Another object of the present invention is the application providing a kind of animal feed to take off mould dose.
Object of the present invention is achieved through the following technical solutions:
A kind of animal feed takes off mould dose, this de-mould dose comprises following component according to the mass fraction: bacillus subtilis (CICC20872) 50 ~ 150 parts, 5 ~ 15 parts, lactobacillus acidophilus freeze-drying powder (CICC6074), sodium selenite 0.1 ~ 1 part, vitamin E2 5 ~ 55 parts, feeding active dry yeast 100 ~ 300 parts, glucomannans 5 ~ 15 parts, montmorillonite 50 ~ 150 parts and improvement hydrated aluminosilicate 350 ~ 700 parts.
Preferred: in this de-mould dose, the content of each component is: bacillus subtilis (CICC20872) 70 ~ 130 parts, 7 ~ 13 parts, lactobacillus acidophilus freeze-drying powder (CICC6074), sodium selenite 0.2 ~ 0.8 part, vitamin E 30 ~ 50 parts, feeding active dry yeast 140 ~ 260 parts, glucomannans 7 ~ 13 parts, montmorillonite 70 ~ 130 parts and improvement hydrated aluminosilicate 400 ~ 650 parts.
Further preferred, in this de-mould dose, the content of each component is: bacillus subtilis (CICC20872) 100 parts, 10 parts, lactobacillus acidophilus freeze-drying powder (CICC6074), sodium selenite 0.55 part, vitamin E 40 parts, feeding active dry yeast 200 parts, glucomannans 10 parts, montmorillonite 100 parts and improvement hydrated aluminosilicate 540 parts.
Described improvement hydrated aluminosilicate adopts following methods preparation: by hydrated aluminosilicate mineral, broken and sieve particle diameter is 3 ~ 10mm mineral through fine crusher, then grind on agate jar, load volume and tank volume are than being 1:1.5 ~ 3, adopt the mode of dry-wet ball mill, front 25 ~ 35h adopts the grinding of dry type ball-milling method, and rear 25 ~ 35h adds absolute ethyl alcohol and adopts the grinding of wet ball-milling method, is dried by sample and obtain improvement hydrated aluminosilicate after grinding.
The addition of above-mentioned absolute ethyl alcohol is: every 50g mineral add the absolute ethyl alcohol of 10 ~ 20ml.
Described improvement hydrated aluminosilicate is preferably improvement hydration ca aluminosilicate sodium salt.
Above-mentioned animal feed takes off the preparation method of mould dose, comprises the following steps:
(1) preparation improvement hydrated aluminosilicate: by hydrated aluminosilicate mineral, broken and sieve particle diameter is 3 ~ 10mm mineral through fine crusher, then grind on agate jar, load volume and tank volume are than being 1:1.5 ~ 3, adopt the mode of dry-wet ball mill, front 25 ~ 35h adopts the grinding of dry type ball-milling method, and rear 25 ~ 35h adds absolute ethyl alcohol and adopts the grinding of wet ball-milling method, is dried by sample and obtain improvement hydrated aluminosilicate after grinding;
(2) by bacillus subtilis (CICC20872), lactobacillus acidophilus freeze-drying powder (CICC6074), sodium selenite, vitamin E, feeding active dry yeast, glucomannans, improvement hydrated aluminosilicate prepared by montmorillonite and step (1) mixes in proportion.
Above-mentioned preparation method, it is that the addition of described absolute ethyl alcohol is: every 50g mineral add the absolute ethyl alcohol of 10 ~ 20ml.
Above-mentioned animal feed takes off the mould dose of application in animal feed.
The content of the bacillus subtilis (CICC20872) in above-mentioned de-mould dose is 1 × 10
10cfu/g, the content of lactobacillus acidophilus freeze-drying powder (CICC6074) is 2 × 10
10cfu/g, vitamin E is 500IU/g.
The present invention development be a kind of green, safety microorganism take off mould dose.This de-mould dose of addition in feed is 0.05% ~ 0.2%, namely adds 0.5 ~ 2kg in the feed of 1 ton and takes off mould dose.
The formula that the present invention takes off mould dose mainly contains two large class components and forms, and one is that the composition that directly can absorb mycotoxin comprises glucomannans, montmorillonite, improvement hydrated aluminosilicate; Equations of The Second Kind can grow and regulate the flora of animal intestinal and trace element to comprise bacillus subtilis, lactobacillus acidophilus, sodium selenite, vitamin E by mould fungus inhibition.Feeding active dry yeasr wherein in formula has absorption concurrently and regulates animal intestinal growth promoting effects double action.
Each material in glucomannans, montmorillonite and improvement hydrated aluminosilicate all has the effect of absorbing mycotoxin, but the limitednumber of its adsorption effect and absorbing mycotoxin, but the composition that above four kinds have de-mould function is combined by inventor, can produce synergistic function, the mycotoxin of the adsorbable overwhelming majority and its adsorption effect significantly increase.
In order to better play the effect of hydrated aluminosilicate, improved treatment being carried out to In Natural Silicate salt material, not only making hole increase, add the area of absorption, also add the speed of its absorption.
Adsorbent can take off mould, but can not the growth of mould fungus inhibition, can not suppress the generation of toxin, and in order to from Sources controlling, we add the adaptable microorganism of feed.These two kinds of bacteriums enter ramp afterwards in body, change intestinal environment, suppress the growth of various pathogen, produce various enzyme, acid and vitamin simultaneously and help animal digestion and absorption.Adding feeding active dry yeasr, sodium selenite and vitamin E in prescription simultaneously, for alleviating the bad reaction of animal, improving its immunity.
This prescription is an organic whole, can not see the function of certain single component, removes any one component in prescription, all can destroy entirety.This prescription can not only remove various mycotoxin, can also promote the growth of animal.Each component in this prescription has the effect of Synergistic.The growth of its detoxification, mould fungus inhibition and the resultant effect of raising animal immunizing power promotion growth of animal are all significantly higher than the effect sum that each component is used alone.
The various common mycotoxin of the glucomannans added in prescription of the present invention, montmorillonite and improvement aluminium hydrosilicate salt pair such as aflatoxin, brown aspertoxin, FT, clavacin toxin, zearalenone, vomiting mycin, T-2 toxin and rotten Ma Su etc. all have extremely strong absorption affinity, and form stable compound, not by intestinal absorption, excrete with ight soil.
The aluminium silicate minerals such as montmorillonite, bentonite, clay as non-nutritive additive, by a large amount of absorption for mycotoxin in feed.About the mechanism of aluminosilicate absorption mycotoxin toxicity, be commonly considered as having adsorbed mycotoxin due to aluminosilicate and having defined stable compound, prevent the absorption of mycotoxin in animal alimentary canal, thus decrease the toxin amount entered in animal body, make its toxicity alleviate or eliminate.These aluminium silicate mineral materials have that hole is many, surface area is large and the characteristic such as ion-exchange capacity is strong, have certain suction-operated to mycotoxin.But these common aluminosilicate material limited sorption capacity, and selective poor.There is good selective adsorption capacity from the hydration ca aluminosilicate sodium salt (HSCAS) of zeolite to AFB1 as extracted, but be not very good to other several main mycotoxin absorbant ability, as not good to the adsorption effect of ZON, OTA, T-2 and DONN.Another shortcoming of HSCAS adsorbent is that it has certain adsorption capacity to vitamin and mineral salt.
In order to overcome above shortcoming, we find after experimental study, carry out the modified HSCAS that can improve to the suction-operated of nonpolar toxin and selective adsorption capacity by applying nano technology to HSCAS.Increase and distribution rule through modified HSCAS nano-complex aperture, specific area increases, and substantially increases its adsorptive selectivity to adsorbed material, selectivity and high efficiency.Mycotoxin in this novel nano compound energy efficient adsorption feed, is stoped it to be entered in body by absorption features, and excretes from ight soil, thus reduces toxin remaining in animal body tissue, alleviates the harmful effect of toxin to growth of animal.
Iturin is the large class lipopeptide compound produced by hay bacillus, family member comprises Iturin A, B, C, D, E, gemma rhzomorph (Bacillomycin) D, F, L and mycosubtilin (Mycosubtilin) etc.The peptide ring that they are all made up of 7 amino acid residues, and the amphoteric compound of beta-amino aliphatic acid side chain composition, they have strong antibacterial ability to most pathogenic yeast and mould.Bacillus subtilis can produce gemma mycin D, suppresses Aspergillus flavus to produce aflatoxin.The antibacterial substance of producing bacillus subtilis life has good heat resistance, if the active material that can be secreted carries out Purification and Characterization, and its active material is used for the control of aflatoxin contamination, brings huge benefit by giving the production of crops.
Exist with spore form with bacillus subtilis at storage, be in the period of dormancy, do not consume the nutritional labeling of feed, do not affect feed quality, quality of the fodder is guaranteed.Bacillus subtilis not only can the generation of mould fungus inhibition toxin, also has a lot of facilitation to growth of animal.
Bacillus sprouts rapidly in enteron aisle changes the auxotype thalline with metabolism into, consumes a large amount of oxygen, maintains the tight oxygen environment of enteron aisle.Bacillus can produce the vegetality carbohydrate of pepsin, lipase, amylase activity, energy degradation of xylan, CMC, polygalacturonase and some other complexity, secretion organic acid promotes the breeding etc. of beneficial bacterium, so bacillus is a kind of feed addictive of pre-anti-diarrhea.In addition the discharge of foul smell can also be reduced.
Yeast also can adsorb mycotoxin, and the assimilation effect of yeast to ochracin is better in acid condition.The absorption affinity of yeast cell wall is especially obvious, because peptide glycan, protein and lipid abundant in cell membrane, provides wide variety, machine-processed different adsorption site for absorbing mycotoxin.P450 in saccharomyces cerevisiae in conjunction with aflatoxin, can reduce the content of B1, B2, G1, G2 effectively.
Lactobacillus acidophilus produces lactic acid and acetic acid, and energy extracellular proteinase, promotes digesting and assimilating of protein.The predigestion of food in stomach can be improved, improve calcium, phosphorus, the utilization rate of iron and the absorption of vitamin D; Also antibiotic can be produced, as acidophilin, acidolin and lactocidin.The a large amount of Cobastab group energys produced promote the growth of nerve cell.
Therefore the present invention has utilized above several composition to make de-mould dose of a kind of green non-pollution.
The feature that the present invention takes off mould dose is exactly multiplely have the component of adsorbing various toxin function and form, and can adsorb common toxin.Another feature that the present invention takes off mould dose adds various active bacterium exactly, and these bacteriums can not only the growth of mould fungus inhibition, the more important thing is that its secretion can promote the growth of animal, regulates functions of intestines and stomach, strengthen the immunity of animal.These bacteriums are all buy from industrial microorganism preservation center, can be applied directly in feed.
Beneficial effect of the present invention:
De-mould dose of green non-pollution prepared by the present invention, all has extremely strong absorption affinity to various common mycotoxin, and forms stable compound, not by intestinal absorption, excrete with ight soil.Of the present invention de-mould dose not only can detoxification, can also the growth of mould fungus inhibition, and improve animal immunizing power and promote growth of animal.
Detailed description of the invention:
Embodiment 1 is prepared animal feed and is taken off mould dose
Preparation improvement hydration ca aluminosilicate sodium salt: first by hydration ca aluminosilicate sodium salt mineral (the white Co., Ltd in purchased from American Mill, effective content more than 95%) then sieving obtains the mineral of 3 ~ 10mm to carry out fragmentation (fertile powerful jaw crusher PEW180 × 1300 of Shanghai dimension) through fine crusher, then grind on agate jar (Nanjing Sang Li Electronic Equipment Factory produces QM-4H ball mill), load with tank volume than being 1:2, adopt the mode of dry-wet ball mill, front 30h adopts dry type ball-milling method (rotating speed is 200r/min) grinding, rear 30h adopts the grinding of wet ball-milling method, method is with dry type ball-milling method, just add the absolute ethyl alcohol (amount that every 50g mineral add) of 12 ~ 15ml during grinding, after grinding, sample is dried.Adopt NEC 2000F type transmission microscopy to observe the particle of grinding, the particle of discovery≤100nm accounts for 78%, 100 ~ 150nm account for 7%, > 150nm account for 15%.This method can make the particle reaching Nano grade account for more than 85%, and therefore the method can prepare nano level particle, namely improves hydration ca aluminosilicate sodium salt.
Take each material by following consumption and mix: bacillus subtilis (CICC20872) 100g(content is 1 × 10
10cfu/g), lactobacillus acidophilus freeze-drying powder (CICC6074) 10g(content is 2 × 10
10cfu/g), sodium selenite 0.55g, vitamin E 40g(500IU/g), feeding active dry yeast 200g(Angel Yeast Co., Ltd) and, glucomannans 10g, montmorillonite 100g, improvement hydration ca aluminosilicate sodium salt 540g.
The main component composition of table 1 0.1% feed addictive
0.1% feed addictive, 1kg adds in the feed of 1 ton.
Comparative example 1
Take each material by following consumption and mix: bacillus subtilis (CICC20872) 100g(content is 1 × 10
10cfu/g), lactobacillus acidophilus freeze-drying powder (CICC6074) 10g(content is 2 × 10
10cfu/g), sodium selenite 0.55g, vitamin E 40g(500IU/g), feeding active dry yeast 200g(Angel Yeast Co., Ltd) and, glucomannans 10g, montmorillonite 100g, natural hydration ca aluminosilicate sodium salt 540g.
Comparative example 2
Take each material by following consumption and mix: bacillus subtilis (CICC20872) 100g(content is 1 × 10
10cfu/g), lactobacillus acidophilus freeze-drying powder (CICC6074) 10g(content is 2 × 10
10cfu/g), sodium selenite 0.55g, vitamin E 40g(500IU/g), feeding active dry yeast 200g(Angel Yeast Co., Ltd) and, montmorillonite 100g.
Test one
1 materials and methods
1.1 test periods and place
This test on June 7 ,-2011 years on the 20th April in 2011 in Shandong Province certain chicken house carry out.
1.2 experimental design
Select the AA+ Breeder hens in 12300 70 week ages, stochastic averagina is divided into test group, control group one, control group two, control group three and blank group, and often organize 3 repetitions, free choice feeding, drinking-water, raise in identical environment.
Test group: basal diet (see table 2), adds mould dose of 0.1% Mei Tuokangtuo (start to add on May 8th, 2011, add 4 weeks) prepared by embodiment 1;
Control group one: basal diet, the amount by 0.1% is added mould complete mould de-mould dose (Beijing Nong Bolier science and technology limited Company lot number 20110304), starts to add, add 4 weeks on May 8th, 2011;
Control group two: basal diet, adds 0.1% de-mould dose (start to add on May 8th, 2011, add 4 weeks) prepared by comparative example 1;
Control group three: basal diet, adds 0.1% de-mould dose (start to add on May 8th, 2011, add 4 weeks) prepared by comparative example 2;
Blank group: basal diet (not adding de-mould dose).
Mould complete mould de-mould dose be Beijing Nong Bolier science and technology limited Company production, and product main component is Meng Tuo city, imported France original producton location, nanometer montomorillonite, lactic acid second fat, sweet poly oligosaccharide, potassium sorbate, ablastins, flavor enhancement etc.
1.3 test indexes measure
Duration of test records and measures the dead naughty number of each group of kind chicken every day, to lay eggs number, defective egg number (broken, abnormal, soft without shell), egg is selected by kind of an egg standard, qualified hatching of breeding eggs, measure and its every production performance index and reproductive performance, comprise egg number, qualified kind of egg number, rate of fertilization, incubation rate, strong young rate, still birth rate etc., and analyze its variation tendency.
Fertility rate of hatching egg=fertile egg number/enter incubation number × 100% hatchability of fertile eggs=chickling-surviving number/fertile egg number × 100%
Strong young rate=total strong young number/chickling-surviving number × 100% still birth rate=stillborn foetus egg number/fertile egg number × 100%
Blood sampling before de-mould dose of interpolation, is taken a blood sample 1 time weekly later, totally 4 times, by HI method, censorship serum is carried out to the detection of newcastle epidemic disease antibody level simultaneously, and then analyzes the impact of inferring improving immunity of organisms aspect.
2 result of the tests
2.1. the de-mould dose of different impacts on Breeder hens production performance is added
As shown in Table 3, the average egg number of test group is 1632.60 pieces, and the average egg number of control group one, control group two and control group three is respectively 1612.74,1607.2,1600.3 pieces, and it is 1592.50 that blank group is on average laid eggs.Relative to blank group, it is 40.1 and 20.24,14.7,7.8 pieces that test group and control group one, control group two, control group three on average increase kind of an egg number every day, improves 2.52% and 1.27%, 0.92%, 0.49% respectively.Plant the disqualification rate of egg relative to blank group, test group and control group one, control group two and control group three have dropped 21.4% and 12.76%, 8.65%, 6.12% respectively.
From strong young data, relative to blank group, test group and control group one, control group two and control group three rise 13.37% and 8.9%, 7.5%, 3.1% respectively.Stillborn foetus number is relative to blank group, and test group and control group one, control group two and control group three have dropped 21.6% and 15.1%, 13.4%, 12.3% respectively.Therefore from above data, use de-mould dose of ratio do not use de-mould dose egg number, qualified egg number, healthy chicken or in survival rate all tool improve a lot.From data, the effect of mould dose of the Mei Tuokangtuo using embodiment 1 to prepare than using mould complete mould de-mould dose and comparative example 1, the Be very effective of comparative example 2 improves.
Table 2 basal diet component list
Table 3 takes off the mould dose of impact on meat chicken production performance
2.2 add the de-mould dose of impact on Breeder hens immune performance.
Can be found out by table 4, after adding de-mould dose the 3rd week, compare blank group, the antibody titer of ND of test group and control group one, control group two and control group three Breeder hens improves 1.4 and 1.1,0.7,0.3 respectively.
Table 4 uses difference to take off mould dose for kind of an impact for antibody IgY against chicken Newcastle Disease level
3 conclusions
Can find out that de-mould dose is laid eggs for kind of a chicken from this experiment, the health of chick has important impact, therefore no matter be whether have mycotoxin in feed, add the de-mould dose of growth for kind of chicken, lay eggs and all there is positive effect, as can be seen from above experimental result also, the effect of mould dose of the Mei Tuokangtuo of our research and development is significantly increased than the effect of de-mould dose in contrast.The effect of de-mould dose adopting improvement hydration ca aluminosilicate sodium salt to make is significantly increased than the effect of de-mould dose adopting natural hydration ca aluminosilicate sodium salt to make.The Be very effective that glucomannans, montmorillonite and improvement hydrated aluminosilicate three kinds of adsorbents comprehensively use combinationally uses or the effect of single use higher than any two kinds of wherein a kind of adsorbent.
Test two
1. test material and method
1.1 test periods and place
Test is carried out on pig farm, Shandong one, and the time is in May, 2012
1.2 experimental design
Stochastic choice is in a good state of health, and body condition is standard type, and the long white Yorkshire binary of the multiparity of parity between 3-5 time is mixed sow 120.Be divided into 3 groups at random, be respectively test group, control group and blank group, each group is respectively 40 repetitions, each repetition 1 sow.
The sow of test group presses mould dose of the Mei Tuokangtuo that 1kg/ ton addition adds embodiment 1 preparation;
Control group adds except mould growth encourage expert takes off mould dose (Beijing You Libao biotechnology Co., Ltd lot number 20120304);
Blank group does not add de-mould dose.
The sow of test group, control group and blank group all processes from the gestational period, terminates to next tire weaning period.Sow all adopts position limiting fence to raise, and breeding wony adopts test-tube mode to carry out.Sow feeds 2.25-2.5kg feed nonpregnant every day in stage, feeds 2-2.25kg feed after breeding in 20d, and after breeding, 2ld returns to the scale of feeding of 2.25-2.5kg until conceived 80d, and conceived 8ld starts scale of feeding and brings up to 3-3.5kg.
In 1.3 tests, sow reproductive performance index observing is comprised:
The endometritis incidence of disease: still have lochia to discharge more than 5d and the sow that secretion is muddy shape is recorded as endometritis, and carry out uterine irrigation and intrauterine administration is treated.Aborning, part sow is had to have obvious endometritis symptom, but Repeat breeding, and usually flow out muddy band liquid frowzy in vagina in oestrus, also endometritis is defined as these sows; Sow Oestrus rate after wean: occur oestrusing in 15d and the sow bred accounts for the head number of total sow after wean; The postweaning estrus time interval: refer to sow after wean to the time interval of oestrusing; Farrowing rate: parturient sow number accounts for the percentage of total breeding sow number.Litter size: whole piglet numbers that sow brood is given birth to, comprises stillborn foetus and mummy tire.The number of live birth: sow give birth to a brood of piglet number whole alive.Survival rate: the number of live birth accounts for the percentage of nest litter size.The average weaning weight of nest piglet: piglet after birth 28 age in days time wean, whole nest pig weight/piglet number.
2 experimental results
The 2.1 de-mould dose of impacts on the Gilt Uterus intimitis incidence of disease
Add in test group 40 sows of mould dose of Mei Tuokangtuo, the sow head number that endometritis occurs is 2, and the incidence of disease is 5%.The sow head number of control group generation endometritis is 5, and the incidence of disease is 12.5%.And the pig's head number of blank group generation endometritis is 6, the incidence of disease is 15%, can find out to add to take off the incidence of disease that mould dose significantly can be reduced endometritis from these group data, and the effect that U.S. holder Kombi takes off mould dose except mould growth encourage expert will be got well.
Table 5 sow basal diet proportioning
Compound additive provides for every Kg daily ration: vitamin A, 5512IU; Vitamine D3,1100IU; Vitamin E, 48IU; Vitamin K3,2.2mg; Vitamin B12,27.6ug; Riboflavin, 5.5mg; D-VB5,13.8mg; Nicotinic acid, 30.3mg
Table 6 takes off the mould dose of impact on the Gilt Uterus intimitis incidence of disease
2.2 de-mould dose on sow postweaning estrus rate and estrus time interval impact
Test group 40 sows of adding mould dose of Mei Tuokangtuo have 35 and oestrus in 15d, and Oestrus rate is 87.5%; And add control group 40 sows taking off mould dose except mould growth encourage expert and have 32 oestrus of sow, Oestrus rate is 80%.40 sows of blank group only have 31 hair feelings, and Oestrus rate is only 77.5%.
Table 7 takes off mould dose to oestrus of sow rate and the impact in the postweaning estrus time interval
The 2.3 de-mould dose of impacts on Farrowing rate and nest litter size
In process of the test, oestrus abnormal because selected sow exists and breed unsuccessful, the sow number of each group actual participation statistics is test group is respectively 35, and control group 32, blank group is 31.Wherein test group sow totally 32 childbirths, parturition rate is 91.4%, control group totally 27 smoothly deliver, parturition rate is 84.3%, and blank group parturition rate is 81.2%.Therefore as can be seen from following table, no matter be from the viewpoint of nest litter size or from survival rate, one group that adds de-mould dose all effective than a group of not adding de-mould dose, and the ratio adding Mei Tuokang adds except mould growth encourage expert takes off mould dose effective
Table 8 takes off the mould dose of impact on Farrowing rate and nest litter size
3 conclusions
As can be seen from the above data de-mould dose for sow, piglet tool has a great impact, and therefore in actual production, no matter whether there is mycotoxin in feed, all will add the de-mould dose of generation that not only can prevent above pathology, can also increase economic efficiency.
Embodiment 2
Take each material by following consumption and mix: bacillus subtilis (CICC20872) 70g(content is 1 × 10
10cfu/g), lactobacillus acidophilus freeze-drying powder (CICC6074) 7g(content is 2 × 10
10cfu/g), sodium selenite 0.2g, vitamin E 30g(500IU/g), feeding active dry yeast 140g(Angel Yeast Co., Ltd), glucomannans 7g, montmorillonite 70g, the improvement hydration ca aluminosilicate sodium salt 400g prepared according to the step described in embodiment 1.
Embodiment 3
Take each material by following consumption and mix: bacillus subtilis (CICC20872) 130g(content is 1 × 10
10cfu/g), lactobacillus acidophilus freeze-drying powder (CICC6074) 13g(content is 2 × 10
10cfu/g), sodium selenite 0.8g, vitamin E 50g(500IU/g), feeding active dry yeast 260g(Angel Yeast Co., Ltd), glucomannans 13g, montmorillonite 130g, the improvement hydration ca aluminosilicate sodium salt 650g prepared according to the step described in embodiment 1.
Embodiment 4
Take each material by following consumption and mix: bacillus subtilis (CICC20872) 50g(content is 1 × 10
10cfu/g), lactobacillus acidophilus freeze-drying powder (CICC6074) 5g(content is 2 × 10
10cfu/g), sodium selenite 0.1g, vitamin E2 5g(500IU/g), feeding active dry yeast 100g(Angel Yeast Co., Ltd), glucomannans 5g, montmorillonite 50g, the improvement hydration ca aluminosilicate sodium salt 350g prepared according to the step described in embodiment 1.
Embodiment 5
Take each material by following consumption and mix: bacillus subtilis (CICC20872) 150g(content is 1 × 10
10cfu/g), lactobacillus acidophilus freeze-drying powder (CICC6074) 15g(content is 2 × 10
10cfu/g), sodium selenite 1g, vitamin E 55g(500IU/g), feeding active dry yeast 300g(Angel Yeast Co., Ltd), glucomannans 15g, montmorillonite 150g, the improvement hydration ca aluminosilicate sodium salt 700g prepared according to the step described in embodiment 1.
Claims (8)
1. an animal feed takes off mould dose, it is characterized in that: this de-mould dose comprises following component according to the mass fraction: bacillus subtilis CICC 20,872 50 ~ 150 parts, lactobacillus acidophilus freeze-drying powder CICC 6,074 5 ~ 15 parts, sodium selenite 0.1 ~ 1 part, vitamin E 25 ~ 55 parts, feeding active dry yeast 100 ~ 300 parts, glucomannans 5 ~ 15 parts, montmorillonite 50 ~ 150 parts and improvement hydrated aluminosilicate 350 ~ 700 parts;
Described improvement hydrated aluminosilicate adopts following methods preparation: by hydrated aluminosilicate mineral, broken and sieve particle diameter is 3 ~ 10mm mineral through fine crusher, then grind on agate jar, load volume and tank volume are than being 1:1.5 ~ 3, adopt the mode of dry-wet ball mill, front 25 ~ 35h adopts the grinding of dry type ball-milling method, and rear 25 ~ 35h adds absolute ethyl alcohol and adopts the grinding of wet ball-milling method, is dried by sample and obtain improvement hydrated aluminosilicate after grinding.
2. animal feed according to claim 1 takes off mould dose, it is characterized in that: in this de-mould dose, the content of each component is: bacillus subtilis CICC 20,872 70 ~ 130 parts, lactobacillus acidophilus freeze-drying powder CICC 6,074 7 ~ 13 parts, sodium selenite 0.2 ~ 0.8 part, vitamin E 30 ~ 50 parts, feeding active dry yeast 140 ~ 260 parts, glucomannans 7 ~ 13 parts, montmorillonite 70 ~ 130 parts and improvement hydrated aluminosilicate 400 ~ 650 parts.
3. animal feed according to claim 2 takes off mould dose, it is characterized in that: in this de-mould dose, the content of each component is: bacillus subtilis CICC 20,872 100 parts, lactobacillus acidophilus freeze-drying powder CICC 6,074 10 parts, sodium selenite 0.55 part, vitamin E 40 parts, feeding active dry yeast 200 parts, glucomannans 10 parts, montmorillonite 100 parts and improvement hydrated aluminosilicate 540 parts.
4. animal feed according to claim 1 takes off mould dose, it is characterized in that the addition of described absolute ethyl alcohol is: every 50g mineral add the absolute ethyl alcohol of 10 ~ 20ml.
5. take off mould dose according to the animal feed in claims 1 to 3 described in any one, it is characterized in that described improvement hydrated aluminosilicate is for improvement hydration ca aluminosilicate sodium salt.
6. the animal feed in claims 1 to 3 described in any one takes off the preparation method of mould dose, it is characterized in that comprising the following steps:
(1) preparation improvement hydrated aluminosilicate: by hydrated aluminosilicate mineral, broken and sieve particle diameter is 3 ~ 10mm mineral through fine crusher, then grind on agate jar, load volume and tank volume are than being 1:1.5 ~ 3, adopt the mode of dry-wet ball mill, front 25 ~ 35h adopts the grinding of dry type ball-milling method, and rear 25 ~ 35h adds absolute ethyl alcohol and adopts the grinding of wet ball-milling method, is dried by sample and obtain improvement hydrated aluminosilicate after grinding;
(2) by bacillus subtilis CICC 20872, lactobacillus acidophilus freeze-drying powder CICC 6074, sodium selenite, vitamin E, feeding active dry yeast, glucomannans, improvement hydrated aluminosilicate prepared by montmorillonite and step (1) mixes in proportion.
7. preparation method according to claim 6, is characterized in that the addition of described absolute ethyl alcohol is: every 50g mineral add the absolute ethyl alcohol of 10 ~ 20ml.
8. the animal feed in claims 1 to 3 described in any one takes off the mould dose of application in animal feed.
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