CN114145388A - Biological mildew removing agent for livestock feed and preparation method thereof - Google Patents
Biological mildew removing agent for livestock feed and preparation method thereof Download PDFInfo
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- CN114145388A CN114145388A CN202111460191.6A CN202111460191A CN114145388A CN 114145388 A CN114145388 A CN 114145388A CN 202111460191 A CN202111460191 A CN 202111460191A CN 114145388 A CN114145388 A CN 114145388A
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- 244000144972 livestock Species 0.000 title claims abstract description 39
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 19
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 19
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 19
- 108010015776 Glucose oxidase Proteins 0.000 claims abstract description 19
- 239000004366 Glucose oxidase Substances 0.000 claims abstract description 19
- 229940116332 glucose oxidase Drugs 0.000 claims abstract description 19
- 235000019420 glucose oxidase Nutrition 0.000 claims abstract description 19
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 15
- 238000005303 weighing Methods 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 5
- 229910021641 deionized water Inorganic materials 0.000 claims description 5
- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical compound O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 239000011259 mixed solution Substances 0.000 claims description 5
- 229910052901 montmorillonite Inorganic materials 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 238000010025 steaming Methods 0.000 claims description 5
- 239000011148 porous material Substances 0.000 claims description 4
- 239000002636 mycotoxin Substances 0.000 abstract description 9
- 231100000678 Mycotoxin Toxicity 0.000 abstract description 8
- 230000004060 metabolic process Effects 0.000 abstract description 5
- 108090000790 Enzymes Proteins 0.000 abstract description 3
- 102000004190 Enzymes Human genes 0.000 abstract description 3
- 229940088598 enzyme Drugs 0.000 abstract description 3
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract description 2
- 239000002068 microbial inoculum Substances 0.000 abstract description 2
- 238000009825 accumulation Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- OQIQSTLJSLGHID-WNWIJWBNSA-N aflatoxin B1 Chemical compound C=1([C@@H]2C=CO[C@@H]2OC=1C=C(C1=2)OC)C=2OC(=O)C2=C1CCC2=O OQIQSTLJSLGHID-WNWIJWBNSA-N 0.000 description 2
- 229930020125 aflatoxin-B1 Natural products 0.000 description 2
- LINOMUASTDIRTM-QGRHZQQGSA-N deoxynivalenol Chemical compound C([C@@]12[C@@]3(C[C@@H](O)[C@H]1O[C@@H]1C=C(C([C@@H](O)[C@@]13CO)=O)C)C)O2 LINOMUASTDIRTM-QGRHZQQGSA-N 0.000 description 2
- 229930002954 deoxynivalenol Natural products 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- MBMQEIFVQACCCH-UHFFFAOYSA-N trans-Zearalenon Natural products O=C1OC(C)CCCC(=O)CCCC=CC2=CC(O)=CC(O)=C21 MBMQEIFVQACCCH-UHFFFAOYSA-N 0.000 description 2
- LINOMUASTDIRTM-UHFFFAOYSA-N vomitoxin hydrate Natural products OCC12C(O)C(=O)C(C)=CC1OC1C(O)CC2(C)C11CO1 LINOMUASTDIRTM-UHFFFAOYSA-N 0.000 description 2
- MBMQEIFVQACCCH-QBODLPLBSA-N zearalenone Chemical compound O=C1O[C@@H](C)CCCC(=O)CCC\C=C\C2=CC(O)=CC(O)=C21 MBMQEIFVQACCCH-QBODLPLBSA-N 0.000 description 2
- 101100449517 Arabidopsis thaliana GRH1 gene Proteins 0.000 description 1
- 206010003591 Ataxia Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 206010006895 Cachexia Diseases 0.000 description 1
- 208000005156 Dehydration Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 206010062016 Immunosuppression Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 101100434479 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) AFB1 gene Proteins 0.000 description 1
- 206010044565 Tremor Diseases 0.000 description 1
- 239000002115 aflatoxin B1 Substances 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000013872 defecation Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 208000026500 emaciation Diseases 0.000 description 1
- 206010016256 fatigue Diseases 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- UVBUBMSSQKOIBE-DSLOAKGESA-N fumonisin B1 Chemical compound OC(=O)C[C@@H](C(O)=O)CC(=O)O[C@H]([C@H](C)CCCC)[C@@H](OC(=O)C[C@@H](CC(O)=O)C(O)=O)C[C@@H](C)C[C@H](O)CCCC[C@@H](O)C[C@H](O)[C@H](C)N UVBUBMSSQKOIBE-DSLOAKGESA-N 0.000 description 1
- QZIADBYRQILELJ-UHFFFAOYSA-N fumonisin B1 Natural products CCCCC(C)C(OC(=O)CC(CC(=O)O)C(=O)O)C(C)(CC(C)CC(O)CCCCC(O)CC(O)C(C)N)OC(=O)CC(CC(=O)O)C(=O)O QZIADBYRQILELJ-UHFFFAOYSA-N 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000001506 immunosuppresive effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- RWQKHEORZBHNRI-BMIGLBTASA-N ochratoxin A Chemical compound C([C@H](NC(=O)C1=CC(Cl)=C2C[C@H](OC(=O)C2=C1O)C)C(O)=O)C1=CC=CC=C1 RWQKHEORZBHNRI-BMIGLBTASA-N 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Inorganic Chemistry (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a livestock feed biological mildew removing agent and a preparation method thereof, wherein the livestock feed biological mildew removing agent comprises 5-10 parts by weight of bacillus subtilis, 8-15 parts by weight of bacillus licheniformis, 7-13 parts by weight of glucose oxidase and 50-80 parts by weight of a carrier. The preparation process can be completed by directly mixing. The invention provides a livestock feed biological mold remover and a preparation method thereof, which adopt the matching of a complex microbial inoculum and enzyme to promote the metabolism of mold in the digestive tract, a carrier can absorb and destroy mycotoxin and is discharged along with physiological metabolism, the accumulation of mold in the livestock is reduced, and the health of the livestock is promoted.
Description
Technical Field
The invention relates to the technical field of livestock breeding, in particular to a livestock feed biological mildew removing agent and a preparation method thereof.
Background
Mycotoxins are a series of toxic metabolites produced by mold in the growth process, mainly comprise aflatoxin B1(AFB1), Zearalenone (ZON), Deoxynivalenol (DON), ochratoxin A (OTA) and fumonisin B1(FMB1) and the like, and widely pollute various feeds and raw materials, endanger the development of cultivation and bring huge economic loss.
Animals ingest mycotoxin-containing feeds or foods and become poisoned, and die from lassitude, slow growth and development, emaciation, appetite reduction and drinking preference, frequent and soft defecation, even diarrhea, dehydration, ataxia, muscle tremor, and even shock, and have a high mortality rate. Among them, the most harmful is immune failure caused by immunosuppression of mycotoxins, resulting in epidemic diseases and significant economic loss.
The problem of mycotoxin contamination is solved from multiple aspects: firstly, culturing an anti-mycotoxin feed variety; secondly, well harvesting and storing, wherein the water content in the feed is generally required to be lower than 10%; thirdly, the contaminated feed is detoxified by adopting a physical, chemical or biological method, so that the utilization rate of the feed is improved. In the actual production, whether the feed is mildewed or not, a certain detoxifying agent is added. Most of the prior mold removers are methods such as physical adsorption or chemical killing, the physical adsorption effect is limited, and a large amount of chemical substances added in the chemical killing have influence on the health of livestock and are difficult to meet the feeding requirement.
Therefore, the problem to be solved by the technical personnel in the field is how to provide a high-efficiency livestock feed biological mold remover without toxic and side effects.
Disclosure of Invention
In view of the above, the invention provides a livestock feed biological mold removal agent and a preparation method thereof, and biological compound bacteria and enzymes are adopted to cooperate to promote mycotoxin metabolism of livestock so as to achieve a good detoxification effect.
In order to achieve the purpose, the invention adopts the following technical scheme:
a biological mildew remover for livestock feed comprises the following components in parts by weight:
5-10 parts of bacillus subtilis, 8-15 parts of bacillus licheniformis and 7-13 parts of glucose oxidase.
Preferably, the carrier also comprises 50-80 parts of the carrier.
Preferably, the support is a porous material.
Preferably, the porous material is montmorillonite powder.
The invention provides a preparation method of the livestock feed biological mildew removing agent, which comprises the following steps:
weighing bacillus subtilis, bacillus licheniformis and glucose oxidase according to the parts by weight, and then uniformly mixing to obtain the biological mildew remover for the livestock feed.
The invention provides another preparation method of the livestock feed biological mold removal agent, which comprises the following steps:
(1) weighing bacillus subtilis, bacillus licheniformis and glucose oxidase according to parts by weight, and then uniformly mixing and dispersing in deionized water;
(2) dispersing the carrier in the mixed solution obtained in the step (1), and then carrying out low-temperature rotary steaming and drying to obtain the livestock feed biological mildew remover.
Through the technical scheme, compared with the prior art, the invention has the beneficial effects that:
the biological mold remover for livestock feed is prepared through compounding composite microbial inoculum and enzyme to promote the metabolism of mold in digestive tract, adsorbing and destroying mycotoxin with carrier, and discharging the mycotoxin with physiological metabolism.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A biological mildew remover for livestock feed comprises the following components in parts by weight:
5 parts of bacillus subtilis, 15 parts of bacillus licheniformis and 7 parts of glucose oxidase.
The preparation method of the biological mildew removing agent comprises the following steps:
weighing bacillus subtilis, bacillus licheniformis and glucose oxidase according to the parts by weight, and then uniformly mixing to obtain the biological mildew remover for the livestock feed.
Example 2
A biological mildew remover for livestock feed comprises the following components in parts by weight:
8 parts of bacillus subtilis, 12 parts of bacillus licheniformis and 10 parts of glucose oxidase.
The preparation method of the biological mildew removing agent comprises the following steps:
weighing bacillus subtilis, bacillus licheniformis and glucose oxidase according to the parts by weight, and then uniformly mixing to obtain the biological mildew remover for the livestock feed.
Example 3
A biological mildew remover for livestock feed comprises the following components in parts by weight:
10 parts of bacillus subtilis, 8 parts of bacillus licheniformis and 13 parts of glucose oxidase.
The preparation method of the biological mildew removing agent comprises the following steps:
weighing bacillus subtilis, bacillus licheniformis and glucose oxidase according to the parts by weight, and then uniformly mixing to obtain the biological mildew remover for the livestock feed.
Example 4
A biological mildew remover for livestock feed comprises the following components in parts by weight:
5 parts of bacillus subtilis, 15 parts of bacillus licheniformis, 7 parts of glucose oxidase and 50 parts of montmorillonite powder.
The preparation method of the biological mildew removing agent comprises the following steps:
(1) weighing bacillus subtilis, bacillus licheniformis and glucose oxidase according to parts by weight, and then uniformly mixing and dispersing in deionized water;
(2) dispersing the carrier in the mixed solution obtained in the step (1), and then carrying out low-temperature rotary steaming and drying to obtain the livestock feed biological mildew remover.
Example 5
A biological mildew remover for livestock feed comprises the following components in parts by weight:
8 parts of bacillus subtilis, 12 parts of bacillus licheniformis, 10 parts of glucose oxidase and 65 parts of montmorillonite powder.
The preparation method of the biological mildew removing agent comprises the following steps:
(1) weighing bacillus subtilis, bacillus licheniformis and glucose oxidase according to parts by weight, and then uniformly mixing and dispersing in deionized water;
(2) dispersing the carrier in the mixed solution obtained in the step (1), and then carrying out low-temperature rotary steaming and drying to obtain the livestock feed biological mildew remover.
Example 6
A biological mildew remover for livestock feed comprises the following components in parts by weight:
10 parts of bacillus subtilis, 8 parts of bacillus licheniformis, 13 parts of glucose oxidase and 80 parts of montmorillonite powder.
The preparation method of the biological mildew removing agent comprises the following steps:
(1) weighing bacillus subtilis, bacillus licheniformis and glucose oxidase according to parts by weight, and then uniformly mixing and dispersing in deionized water;
(2) dispersing the carrier in the mixed solution obtained in the step (1), and then carrying out low-temperature rotary steaming and drying to obtain the livestock feed biological mildew remover.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (6)
1. The biological mold remover for the livestock feed is characterized by comprising the following components in parts by weight:
5-10 parts of bacillus subtilis, 8-15 parts of bacillus licheniformis and 7-13 parts of glucose oxidase.
2. The livestock feed biological mold remover according to claim 1, further comprising 50-80 parts of a carrier.
3. The livestock feed biological mold remover according to claim 2, wherein the carrier is a porous material.
4. The livestock feed biological mold removal agent as claimed in claim 3, wherein said porous material is montmorillonite powder.
5. The preparation method of the livestock feed biological mold remover as claimed in claim 1, which comprises the following steps:
weighing bacillus subtilis, bacillus licheniformis and glucose oxidase according to the parts by weight, and then uniformly mixing to obtain the biological mildew remover for the livestock feed.
6. A method for preparing the livestock feed biological mold remover as claimed in claim 2, which comprises the following steps:
(1) weighing bacillus subtilis, bacillus licheniformis and glucose oxidase according to parts by weight, and then uniformly mixing and dispersing in deionized water;
(2) dispersing the carrier in the mixed solution obtained in the step (1), and then carrying out low-temperature rotary steaming and drying to obtain the livestock feed biological mildew remover.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111460191.6A CN114145388A (en) | 2021-12-02 | 2021-12-02 | Biological mildew removing agent for livestock feed and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111460191.6A CN114145388A (en) | 2021-12-02 | 2021-12-02 | Biological mildew removing agent for livestock feed and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114145388A true CN114145388A (en) | 2022-03-08 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111460191.6A Pending CN114145388A (en) | 2021-12-02 | 2021-12-02 | Biological mildew removing agent for livestock feed and preparation method thereof |
Country Status (1)
| Country | Link |
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| CN (1) | CN114145388A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103461677A (en) * | 2013-09-25 | 2013-12-25 | 南京日升昌生物技术有限公司 | Mold removal agent for animal feed as well as preparation method and application of mold removal agent |
| CN103535524A (en) * | 2013-09-30 | 2014-01-29 | 宝鸡市星星协力生物有限公司 | Compound type disinfecting mold-removal additive for feed |
| CN107772195A (en) * | 2016-08-27 | 2018-03-09 | 瑞普（天津）生物药业有限公司 | One kind poultry poultry fodder takes off mould dose and preparation method thereof |
-
2021
- 2021-12-02 CN CN202111460191.6A patent/CN114145388A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103461677A (en) * | 2013-09-25 | 2013-12-25 | 南京日升昌生物技术有限公司 | Mold removal agent for animal feed as well as preparation method and application of mold removal agent |
| CN103535524A (en) * | 2013-09-30 | 2014-01-29 | 宝鸡市星星协力生物有限公司 | Compound type disinfecting mold-removal additive for feed |
| CN107772195A (en) * | 2016-08-27 | 2018-03-09 | 瑞普（天津）生物药业有限公司 | One kind poultry poultry fodder takes off mould dose and preparation method thereof |
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| PB01 | Publication | ||
| PB01 | Publication | ||
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| RJ01 | Rejection of invention patent application after publication | ||
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Application publication date: 20220308 |